RchiOBHm_Chr5g0064081

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Forward (+)
69862758 .. 69867981
5224 bp
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UTR
Exon/CDS
Intron
PRQ34015

Sequence Viewer

Length: 3513 bp
ATGGCTTTGAGCATCCCAAGTGCTTCTGCTCGTCAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTGAAACTCGCCATGGTTTTGTATCCCATTTATATGATGAATTGGAAAGCACAGGAATGATTAGCACATTCATGGATGAGGAAAAGCTGGAAGTAGGGAAGGCTATTTCTCCAAATCTTGTAATGGCAATTGAAGAATCAAGGTTTGCTATAGTTGTTCTGTCGCCAAACTATGCTTCTTCTCCTTGGTGTTTGGATGAACTTGCAAAGGTTCTTCAATCCATGGAAGGGAGGGACAGAATCCTGCCAATTTTCTATCATGTGGATCCTTCTCATGTACGACATCAACTGGGGAGTTTTGCAGAAGCCTTTACTAAGCATGAAGAAAATTCTAGGCACAGCAAAGAGAAGTTGGACCAGTGGAGACTTGCTTTAAAAAATGTGGCCGATCTCTCTGGATGGGATGCAAAGAATTTGCGGTCTGAAAGAGAACTTGTTAAACGCATTGTGAAATTTGTCTGCAATAAGGCACTTCAAGTATCACCACGGCCTATTCCAATTGAGTTCAAAGCGCAGCCTACCGAATATATTGAATCAACAACACGGGCCAGGGATAAGCTTACTCAGCTGCTGAAAGATAATGAAATTACTGTCATCGGGGTTTTTGGCAAGGGAGGTGTTGGCAAGAGAACTATGGTGAAACATGTCGGCGCGCAAGCCCGAGAACTTAGGCTTGTTCATTATGTGATTAGGGCTGTTGTAGCCCAGAGCCCTGATTTGAGAAATATTCAAAGCACATTGGCCAATCAGCTGGGCTTAAAATTCGAGGAGGAGGAGACAGAAATTGGAAGAGCCGCTAGATTGAAGGAGAGGATAATGGGAGGAAATAAAATCCTCATAGTCTTAGTCGACATTTGGCGGAGAATAGATTTGTCAAGCATAGGAATTCCCAATTACAACGAGCTTCAAAGACACAATTCCAAACTCATACTAACCTCAAGGAGCAGAAGTGTTTGCTATTCCATGCAGTGCCAAGCAAGCATTCCTCTCCCTATCTTATCAGAAGAAGATTCTTGGAACCTTTTTGTGGAGAAATCGGGAAAGTCTTTTCATGAATCTACCGAGCTGTATGATGTAGCAAGGACCGTAGCTAGAGAATGTGGTGGTCTCCCACTTGCACTGATAACAGTTGCTGGGGTACTTGGAGATAAAGACATAGAAGACTGGAAAGAAGCAGCTCAACGACTAGACAGGTCGCAAACAGTCAATCTTGAGGATGAGGGAGATGTATACAAATGCATAAAGTTCAGCTATGATTACTTGAGATCTGATGATGCCAAATCATTCTTCTTGCTATGCTGTCTATTCCCAGAAGACTATGATATCCCATTCGAAGACTTGCTCAAGTATGCAATTGGGAAAGGGTTGCTTCAAGATGCCAACACAATCCAAGAAGCCAGAGCAACAGCAAATTTGGTGATCAAGTCCCTGAAAGCTTTCAGCCTACTTTTGGAAGGTACAAAGGAGGGATGTGTAAGGATGCATGATGTAGTGCGGGATGTAGCCCTGTCGATTTCGTTTTCTGAAGATGGTCATAAATTTTTGGTAAAAGCTGGTTGTGAATTACAGCATTGGCCAAGGATTAAAGCGCATGGAGGCTATTCTGCAATCTCACTAATGAGGAACAAAATTTGCAAGCTTCCCAAAGAAGAATCACCATCTAGAGACTACTCTTCAAAACCTTATTGCTCACATGATCTTTCAAGAACCTTGACTCTTAGCACAACAATCAACACCTTACCAGATTGGTTTATCAAAGTGGTAGCAGAGAAAGCAGAGAAACTACAGTACCTAAAGTGCAAAGGCCTAAAGAACATTCTTGTGGAACATGACAGAGGGAGGTTACATGGACTGAAGTATCTAGCTGTAATTGGGCCCCATGAGTACTTGGCAGTGTTGATGGAAACAATAACACGGGTGACAAATGTATCTGTGTTCAAGAACTTGGAAGAGCTGCATCTCCTTGGTGTGAGTTGTCTGAAGGAGTTATGTGTGGGTGAATTACCACTTGGGTCTCTATTCAATCTCAAGTCATTAAAGGTGGAATATTGCCATGACTTGGTGAATGCACTTTTGTCATCAAATTTGTTGCAAAGACTACAAAATCTGGAAAAACTTCATTGTGAAGACATGAATGTTATGGAGTATGTGTTTGGATTTGAAGGGCTGGAGCCAGAAGACATTATCTTGCCAAAATTGAGGGAGATGATATTGTGGGATCTAGAAAAACTAATTAGCATATGGAGTGGTCCTGCTCCAAATGCAGTCTTCCATAATCTTAAAAGTTTGGCAGTCTCAGGTTGCAAAAAACTTGAAAGTCTCTTTACATATGAGGTAGCTAAATGTCTTTTGCAATTGGAAGACCTTTCGGTAACTCAATGTCCTAGCTTGGACATGGTTATTAAATCAAGCAAGGAAGTAGTGGACAAGAAGATCATTTTCCCAGAATTGAGGAACTTAGCTTTGTCGTATCTTCCACGGATCACTAGGTTCTGCAGTAGTGGAATTGCTACCATGGAGTGCCCTTCATTGGAACATTTACATGTGCAGGACTGCCCCCATTTTACAACCTCTACTTCTGACTTCCACTGCTTGAAGCAATTTCAAGTCAATGATCTACGACATTTGAGAATGAGCAAAGACATGGATAACATCAATTTAGCTGGCCAGCATACCAAGAACATCCTTGAGAGCCTACCTCAGAACGTCAGAAAGCGGGTTGAGGTCATTAGAGAAATTGAGAACCAACATCATGAATTAGAGGCAGAGTTTTTCAAGGAGAGCGCTTTGCTTGAAGCTAAGTATCATAAATTATATCAACCTCTCTACAGCAAGCGATACTTGATTGTGAATGGTGTTGTTGAAGCTGAAGGAGTTGCCACTGAAGCAGTAGCAGATCAAGAGGACAAAACTGCAAAAGAGAGAGGAGTGCCTGATTTCTGGCTCAATGCATTGAAGAACAATGAATTGCTAGCCAAGGAGATTACTGAGCATGATGAAGGAGCTCTTAAATATCTGAGAGATATCAAGTGGTTTAAGATACATAATTCCAAGGGGTTCAAACTTCAGTTCTACTTTGACACCAATCCATTTTTCAAAAACTCTGTTTTGACAAAGACATATCACATGATTGATGAAGATAAACCAAATAACGTAGCAGGGACGGAGATTGAATGGTATCCAGCAAAATGCCCGACACAGAAGATTCTTAAGAAGAAGCGTAAGAAGGGATCGAAGAATTCTAAGCCCCTGACTAAAACTGAAAATTGTAAAAGTTTCTTCAATTTGTTTAGTCCACCTCAAGTCTCCAAGGACGATGAAGTTATTGATGAATTTGCTGCCGAGGAACTCAAAAACCAGAAGAAACATGACTATGATGTCGGGTCAACCATTTGTTATAAGATCATCCCACATGCAGTTTCTTGGTTTACTGGAGAAGCTTTTTAG

Protein Analysis

1170

Amino Acids

133.42

Weight (kDa)

6.87

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 183 1.3e-49 TIR domain
TIR_2 PF13676 17 - 112 1.4e-17 TIR domain
NB-ARC PF00931 209 - 369 2.3e-22 NB-ARC domain
LRR_RPS2 PF23247 671 - 816 6.3e-31 Plant disease resistance protein RPS2-like, leucine-rich repeats
NAP PF00956 927 - 1168 5.5e-74 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3465
AasI GACNNNNNNGTC 1 cut(s) 3443
AccB7I CCANNNNNTGG 1 cut(s) 2125
AccI GTMKAC 2 cut(s) 915, 1296
AccII CGCG 1 cut(s) 719
AciI CCGC 5 cut(s) 484, 861, 925, 1561, 2782
AclWI GGATC 5 cut(s) 326, 339, 2292, 2555, 3304
AcoI YGGCCR 4 cut(s) 450, 807, 1640, 2731
AcuI CTGAAG 6 cut(s) 1611, 1940, 2066, 2955, 2970, 3116
AfaI GTAC 5 cut(s) 345, 1206, 1525, 1856, 1952
AfeI AGCGCT 1 cut(s) 2851
AfiI CCNNNNNNNGG 5 cut(s) 294, 1093, 1516, 2125, 2596
AflII CTTAAG 1 cut(s) 3275
AflIII ACRYGT 2 cut(s) 709, 2608
AjnI CCWGG 1 cut(s) 614
Alw21I GWGCWC 1 cut(s) 3073
Alw26I GTCTC 8 cut(s) 424, 836, 1178, 1725, 2085, 2365, 2390, 3376
AlwI GGATC 5 cut(s) 326, 339, 2292, 2555, 3304
AlwNI CAGNNNCTG 2 cut(s) 637, 1199
Ama87I CYCGRG 1 cut(s) 726
Aor51HI AGCGCT 1 cut(s) 2851
AoxI GGCC 8 cut(s) 450, 554, 612, 807, 1640, 1870, 1940, 2731
ApaI GGGCCC 1 cut(s) 1944
ApeKI GCWGC 5 cut(s) 580, 634, 1241, 2020, 3404
Asp700I GAANNNNTTC 2 cut(s) 1502, 2181
AspLEI GCGC 5 cut(s) 580, 719, 721, 1657, 2852
AspS9I GGNCC 6 cut(s) 421, 612, 1149, 1940, 1941, 2315
AsuHPI GGTGA 8 cut(s) 80, 540, 715, 1495, 1713, 1996, 2075, 2140
AsuII TTCGAA 1 cut(s) 1398
AsuNHI GCTAGC 1 cut(s) 3037
AvaI CYCGRG 1 cut(s) 726
AvaII GGWCC 3 cut(s) 421, 1149, 2315
BaeGI GKGCMC 2 cut(s) 1944, 2591
BaeI ACNNNNGTAYC 6 cut(s) 1838, 1871, 1907, 1940, 1977, 2010
BalI TGGCCA 3 cut(s) 809, 1642, 2733
BamHI GGATCC 1 cut(s) 331
BanII GRGCYC 3 cut(s) 779, 1944, 3073
BbsI GAAGAC 7 cut(s) 1233, 1386, 1407, 2199, 2250, 2326, 2433
Bbv12I GWGCWC 1 cut(s) 3073
BbvI GCAGC 5 cut(s) 592, 621, 1253, 2007, 3391
BccI CCATC 4 cut(s) 459, 1589, 1732, 1960
BceAI ACGGC 1 cut(s) 569
BciT130I CCWGG 1 cut(s) 616
BciVI GTATCC 2 cut(s) 100, 3255
BclI TGATCA 1 cut(s) 1485
BcoDI GTCTC 8 cut(s) 424, 836, 1178, 1725, 2085, 2365, 2390, 3376
BfmI CTRYAG 4 cut(s) 216, 1850, 2560, 2893
BfoI RGCGCY 1 cut(s) 2853
BfrI CTTAAG 1 cut(s) 3275
BfuI GTATCC 2 cut(s) 100, 3255
BglII AGATCT 1 cut(s) 1331
BisI GCNGC 6 cut(s) 581, 635, 861, 1242, 2021, 3405
BlsI GCNGC 6 cut(s) 582, 636, 862, 1243, 2022, 3406
BmcAI AGTACT 1 cut(s) 1952
Bme1390I CCNGG 1 cut(s) 616
Bme18I GGWCC 3 cut(s) 421, 1149, 2315
BmeT110I CYCGRG 1 cut(s) 726
BmgT120I GGNCC 6 cut(s) 421, 612, 1149, 1940, 1941, 2315
BmiI GGNNCC 5 cut(s) 333, 1085, 1942, 1943, 2238
BmrFI CCNGG 1 cut(s) 616
BmrI ACTGGG 1 cut(s) 365
BmsI GCATC 6 cut(s) 21, 460, 1330, 1432, 1536, 2032
BmtI GCTAGC 1 cut(s) 3041
BmuI ACTGGG 1 cut(s) 365
BpiI GAAGAC 7 cut(s) 1233, 1386, 1407, 2199, 2250, 2326, 2433
BplI GAGNNNNNCTC 2 cut(s) 2749, 2781
BpmI CTGGAG 1 cut(s) 2255
Bpu14I TTCGAA 1 cut(s) 1398
BpuEI CTTGAG 7 cut(s) 988, 1298, 1348, 1394, 2078, 2774, 3351
BsaI GGTCTC 2 cut(s) 1178, 2085
BsaXI ACNNNNNCTCC 4 cut(s) 1000, 1030, 2201, 2231
Bsc4I CCNNNNNNNGG 5 cut(s) 294, 1093, 1516, 2125, 2596
Bse1I ACTGG 4 cut(s) 360, 424, 1235, 3502
BseBI CCWGG 1 cut(s) 616
BseLI CCNNNNNNNGG 5 cut(s) 294, 1093, 1516, 2125, 2596
BseMII CTCAG 5 cut(s) 644, 2376, 2780, 3045, 3074
BseNI ACTGG 4 cut(s) 360, 424, 1235, 3502
BsePI GCGCGC 1 cut(s) 717
BseRI GAGGAG 4 cut(s) 848, 851, 854, 3006
BseSI GKGCMC 2 cut(s) 1944, 2591
BseXI GCAGC 5 cut(s) 592, 621, 1253, 2007, 3391
BseYI CCCAGC 2 cut(s) 817, 1199
BsgI GTGCAG 1 cut(s) 2633
Bsh1236I CGCG 1 cut(s) 719
BshFI GGCC 8 cut(s) 452, 556, 614, 809, 1642, 1872, 1942, 2733
BsiHKAI GWGCWC 1 cut(s) 3073
BsiHKCI CYCGRG 1 cut(s) 726
BslFI GGGAC 3 cut(s) 314, 1477, 3241
BslI CCNNNNNNNGG 5 cut(s) 294, 1093, 1516, 2125, 2596
BsmAI GTCTC 8 cut(s) 424, 836, 1178, 1725, 2085, 2365, 2390, 3376
BsmFI GGGAC 3 cut(s) 314, 1477, 3241
BsmI GAATGC 2 cut(s) 1047, 2137
BsnI GGCC 8 cut(s) 452, 556, 614, 809, 1642, 1872, 1942, 2733
Bso31I GGTCTC 2 cut(s) 1178, 2085
BsoBI CYCGRG 1 cut(s) 726
Bsp119I TTCGAA 1 cut(s) 1398
Bsp120I GGGCCC 1 cut(s) 1940
Bsp1286I GDGCHC 4 cut(s) 779, 1944, 2591, 3073
Bsp19I CCATGG 3 cut(s) 79, 288, 2580
BspACI CCGC 5 cut(s) 484, 861, 925, 1561, 2782
BspANI GGCC 8 cut(s) 452, 556, 614, 809, 1642, 1872, 1942, 2733
BspCNI CTCAG 5 cut(s) 643, 2375, 2779, 3046, 3075
BspFNI CGCG 1 cut(s) 719
BspHI TCATGA 2 cut(s) 1117, 2818
BspLI GGNNCC 5 cut(s) 333, 1085, 1942, 1943, 2238
BspMAI CTGCAG 1 cut(s) 2564
BspOI GCTAGC 1 cut(s) 3041
BspPI GGATC 5 cut(s) 326, 339, 2292, 2555, 3304
BspQI GCTCTTC 2 cut(s) 850, 2010
BspT104I TTCGAA 1 cut(s) 1398
BspTI CTTAAG 1 cut(s) 3275
BspTNI GGTCTC 2 cut(s) 1178, 2085
BsrI ACTGG 4 cut(s) 360, 424, 1235, 3502
BssHII GCGCGC 1 cut(s) 717
BssNAI GTATAC 1 cut(s) 1297
BssT1I CCWWGG 9 cut(s) 79, 251, 288, 1643, 2029, 2580, 3042, 3117, 3375
Bst1107I GTATAC 1 cut(s) 1297
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 5 cut(s) 658, 1153, 1195, 1270, 1854
Bst6I CTCTTC 3 cut(s) 850, 1744, 2010
BstAFI CTTAAG 1 cut(s) 3275
BstBI TTCGAA 1 cut(s) 1398
BstC8I GCNNGC 8 cut(s) 719, 723, 1045, 1703, 2731, 2735, 2900, 3039
BstDSI CCRYGG 5 cut(s) 79, 288, 551, 2543, 2580
BstFNI CGCG 1 cut(s) 719
BstH2I RGCGCY 1 cut(s) 2853
BstHHI GCGC 5 cut(s) 580, 719, 721, 1657, 2852
BstMAI GTCTC 8 cut(s) 424, 836, 1178, 1725, 2085, 2365, 2390, 3376
BstMWI GCNNNNNNNGC 6 cut(s) 631, 767, 1044, 1838, 2327, 2951
BstNI CCWGG 1 cut(s) 616
BstNSI RCATGY 3 cut(s) 713, 2612, 3482
BstSCI CCNGG 1 cut(s) 614
BstSFI CTRYAG 4 cut(s) 216, 1850, 2560, 2893
BstSLI GKGCMC 2 cut(s) 1944, 2591
BstUI CGCG 1 cut(s) 719
BstV1I GCAGC 5 cut(s) 592, 621, 1253, 2007, 3391
BstV2I GAAGAC 7 cut(s) 1233, 1386, 1407, 2199, 2250, 2326, 2433
BstX2I RGATCY 3 cut(s) 331, 1331, 2284
BstXI CCANNNNNNTGG 1 cut(s) 817
BstYI RGATCY 3 cut(s) 331, 1331, 2284
BstZ17I GTATAC 1 cut(s) 1297
BsuI GTATCC 2 cut(s) 100, 3255
BsuRI GGCC 8 cut(s) 452, 556, 614, 809, 1642, 1872, 1942, 2733
BtgI CCRYGG 5 cut(s) 79, 288, 551, 2543, 2580
BtsI GCAGTG 3 cut(s) 1040, 1965, 2653
BtsIMutI CAGTG 7 cut(s) 41, 431, 1040, 1184, 1965, 2653, 2946
Cac8I GCNNGC 8 cut(s) 719, 723, 1045, 1703, 2731, 2735, 2900, 3039
CaiI CAGNNNCTG 2 cut(s) 637, 1199
CciI TCATGA 2 cut(s) 1117, 2818
CfoI GCGC 5 cut(s) 580, 719, 721, 1657, 2852
Cfr13I GGNCC 6 cut(s) 421, 612, 1149, 1940, 1941, 2315
Csp6I GTAC 5 cut(s) 344, 1205, 1524, 1855, 1951
CviQI GTAC 5 cut(s) 344, 1205, 1524, 1855, 1951
DraI TTTAAA 1 cut(s) 441
DrdI GACNNNNNNGTC 1 cut(s) 3443
DseDI GACNNNNNNGTC 1 cut(s) 3443
EaeI YGGCCR 4 cut(s) 450, 807, 1640, 2731
Eam1104I CTCTTC 3 cut(s) 850, 1744, 2010
EarI CTCTTC 3 cut(s) 850, 1744, 2010
EciI GGCGGA 1 cut(s) 940
Ecl136II GAGCTC 1 cut(s) 3071
Eco130I CCWWGG 9 cut(s) 79, 251, 288, 1643, 2029, 2580, 3042, 3117, 3375
Eco147I AGGCCT 1 cut(s) 1872
Eco24I GRGCYC 3 cut(s) 779, 1944, 3073
Eco31I GGTCTC 2 cut(s) 1178, 2085
Eco32I GATATC 2 cut(s) 1390, 3091
Eco47I GGWCC 3 cut(s) 421, 1149, 2315
Eco47III AGCGCT 1 cut(s) 2851
Eco53kI GAGCTC 1 cut(s) 3071
Eco57I CTGAAG 6 cut(s) 1611, 1940, 2066, 2955, 2970, 3116
Eco88I CYCGRG 1 cut(s) 726
EcoICRI GAGCTC 1 cut(s) 3071
EcoO109I RGGNCCY 1 cut(s) 1941
EcoRI GAATTC 2 cut(s) 951, 3304
EcoRII CCWGG 1 cut(s) 614
EcoRV GATATC 2 cut(s) 1390, 3091
EcoT14I CCWWGG 9 cut(s) 79, 251, 288, 1643, 2029, 2580, 3042, 3117, 3375
EcoT22I ATGCAT 3 cut(s) 1307, 1551, 3019
EcoT38I GRGCYC 3 cut(s) 779, 1944, 3073
ErhI CCWWGG 9 cut(s) 79, 251, 288, 1643, 2029, 2580, 3042, 3117, 3375
FalI AAGNNNNNCTT 6 cut(s) 1419, 1451, 2891, 2923, 3057, 3089
FaqI GGGAC 3 cut(s) 314, 1477, 3241
FauI CCCGC 2 cut(s) 1554, 2775
FauNDI CATATG 2 cut(s) 2306, 2395
FbaI TGATCA 1 cut(s) 1485
FblI GTMKAC 2 cut(s) 915, 1296
Fnu4HI GCNGC 6 cut(s) 581, 635, 861, 1242, 2021, 3405
FriOI GRGCYC 3 cut(s) 779, 1944, 3073
Fsp4HI GCNGC 6 cut(s) 581, 635, 861, 1242, 2021, 3405
GlaI GCGC 5 cut(s) 579, 718, 720, 1656, 2851
GluI GCNGC 6 cut(s) 581, 635, 861, 1242, 2021, 3405
GsaI CCCAGC 2 cut(s) 821, 1203
GsuI CTGGAG 1 cut(s) 2255
HaeII RGCGCY 1 cut(s) 2853
HaeIII GGCC 8 cut(s) 452, 556, 614, 809, 1642, 1872, 1942, 2733
HhaI GCGC 5 cut(s) 580, 719, 721, 1657, 2852
Hin6I GCGC 5 cut(s) 578, 717, 719, 1655, 2850
HinP1I GCGC 5 cut(s) 578, 717, 719, 1655, 2850
HincII GTYRAC 2 cut(s) 916, 3453
HindII GTYRAC 2 cut(s) 916, 3453
HindIII AAGCTT 4 cut(s) 623, 1500, 1703, 3504
HinfI GANTC 8 cut(s) 203, 306, 599, 1076, 1121, 1718, 1780, 3271
HphI GGTGA 8 cut(s) 80, 540, 715, 1495, 1713, 1996, 2075, 2140
Hpy166II GTNNAC 6 cut(s) 916, 1297, 2491, 3362, 3453, 3495
Hpy188I TCNGA 9 cut(s) 490, 1069, 1336, 1591, 2046, 2647, 2769, 2777, 3084
Hpy8I GTNNAC 6 cut(s) 916, 1297, 2491, 3362, 3453, 3495
HpyCH4III ACNGT 5 cut(s) 658, 1153, 1195, 1270, 1854
HpyCH4IV ACGT 2 cut(s) 2772, 3219
HpyF10VI GCNNNNNNNGC 6 cut(s) 631, 767, 1044, 1838, 2327, 2951
HpySE526I ACGT 2 cut(s) 2772, 3219
HspAI GCGC 5 cut(s) 578, 717, 719, 1655, 2850
Ksp22I TGATCA 1 cut(s) 1485
LguI GCTCTTC 2 cut(s) 850, 2010
LmnI GCTCC 4 cut(s) 1008, 2236, 2326, 3068
Lsp1109I GCAGC 5 cut(s) 592, 621, 1253, 2007, 3391
LweI GCATC 6 cut(s) 21, 460, 1330, 1432, 1536, 2032
MaeII ACGT 2 cut(s) 2772, 3219
MaeIII GTNAC 3 cut(s) 1908, 1984, 2437
MfeI CAATTG 4 cut(s) 195, 564, 1419, 2420
MflI RGATCY 3 cut(s) 331, 1331, 2284
MhlI GDGCHC 4 cut(s) 779, 1944, 2591, 3073
MlsI TGGCCA 3 cut(s) 809, 1642, 2733
MluNI TGGCCA 3 cut(s) 809, 1642, 2733
MlyI GAGTC 1 cut(s) 1774
MmeI TCCRAC 1 cut(s) 399
Mox20I TGGCCA 3 cut(s) 809, 1642, 2733
Mph1103I ATGCAT 3 cut(s) 1307, 1551, 3019
MroXI GAANNNNTTC 2 cut(s) 1502, 2181
MscI TGGCCA 3 cut(s) 809, 1642, 2733
MslI CAYNNNNRTG 6 cut(s) 99, 122, 137, 1886, 2607, 3438
Msp20I TGGCCA 3 cut(s) 809, 1642, 2733
MspA1I CMGCKG 2 cut(s) 634, 817
MspCI CTTAAG 1 cut(s) 3275
MspR9I CCNGG 1 cut(s) 616
MunI CAATTG 4 cut(s) 195, 564, 1419, 2420
Mva1269I GAATGC 2 cut(s) 1047, 2137
MvaI CCWGG 1 cut(s) 616
MvnI CGCG 1 cut(s) 719
MwoI GCNNNNNNNGC 6 cut(s) 631, 767, 1044, 1838, 2327, 2951
NcoI CCATGG 3 cut(s) 79, 288, 2580
NdeI CATATG 2 cut(s) 2306, 2395
NheI GCTAGC 1 cut(s) 3037
NlaIV GGNNCC 5 cut(s) 333, 1085, 1942, 1943, 2238
NmeAIII GCCGAG 1 cut(s) 3433
NmuCI GTSAC 1 cut(s) 1984
NsiI ATGCAT 3 cut(s) 1307, 1551, 3019
NspI RCATGY 3 cut(s) 713, 2612, 3482
NspV TTCGAA 1 cut(s) 1398
PagI TCATGA 2 cut(s) 1117, 2818
PauI GCGCGC 1 cut(s) 717
PceI AGGCCT 1 cut(s) 1872
PciI ACATGT 2 cut(s) 709, 2608
PciSI GCTCTTC 2 cut(s) 850, 2010
PctI GAATGC 2 cut(s) 1047, 2137
PdmI GAANNNNTTC 2 cut(s) 1502, 2181
PfeI GAWTC 7 cut(s) 203, 306, 599, 1076, 1121, 1718, 3271
PflMI CCANNNNNTGG 1 cut(s) 2125
PkrI GCNGC 6 cut(s) 582, 636, 862, 1243, 2022, 3406
PleI GAGTC 1 cut(s) 1774
PpsI GAGTC 1 cut(s) 1774
PscI ACATGT 2 cut(s) 709, 2608
PsiI TTATAA 1 cut(s) 3465
Psp124BI GAGCTC 1 cut(s) 3073
Psp6I CCWGG 1 cut(s) 614
PspFI CCCAGC 2 cut(s) 817, 1199
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 5 cut(s) 333, 1085, 1942, 1943, 2238
PspOMI GGGCCC 1 cut(s) 1940
PspPI GGNCC 6 cut(s) 421, 612, 1149, 1940, 1941, 2315
PstI CTGCAG 1 cut(s) 2564
PstNI CAGNNNCTG 2 cut(s) 637, 1199
PsuI RGATCY 3 cut(s) 331, 1331, 2284
PteI GCGCGC 1 cut(s) 717
PvuII CAGCTG 2 cut(s) 634, 817
RsaI GTAC 5 cut(s) 345, 1206, 1525, 1856, 1952
RsaNI GTAC 5 cut(s) 344, 1205, 1524, 1855, 1951
RseI CAYNNNNRTG 6 cut(s) 99, 122, 137, 1886, 2607, 3438
SacI GAGCTC 1 cut(s) 3073
SalI GTCGAC 1 cut(s) 914
SapI GCTCTTC 2 cut(s) 850, 2010
SatI GCNGC 6 cut(s) 581, 635, 861, 1242, 2021, 3405
Sau96I GGNCC 6 cut(s) 421, 612, 1149, 1940, 1941, 2315
ScaI AGTACT 1 cut(s) 1952
SchI GAGTC 1 cut(s) 1774
ScrFI CCNGG 1 cut(s) 616
SduI GDGCHC 4 cut(s) 779, 1944, 2591, 3073
SfaNI GCATC 6 cut(s) 21, 460, 1330, 1432, 1536, 2032
SfcI CTRYAG 4 cut(s) 216, 1850, 2560, 2893
SfuI TTCGAA 1 cut(s) 1398
SinI GGWCC 3 cut(s) 421, 1149, 2315
SmiMI CAYNNNNRTG 6 cut(s) 99, 122, 137, 1886, 2607, 3438
SmlI CTYRAG 8 cut(s) 1003, 1277, 1327, 1409, 2093, 2753, 3275, 3366
SmoI CTYRAG 8 cut(s) 1003, 1277, 1327, 1409, 2093, 2753, 3275, 3366
SseBI AGGCCT 1 cut(s) 1872
SsiI CCGC 5 cut(s) 484, 861, 925, 1561, 2782
SspI AATATT 2 cut(s) 793, 2114
SstI GAGCTC 1 cut(s) 3073
StuI AGGCCT 1 cut(s) 1872
StyD4I CCNGG 1 cut(s) 614
StyI CCWWGG 9 cut(s) 79, 251, 288, 1643, 2029, 2580, 3042, 3117, 3375
TaaI ACNGT 5 cut(s) 658, 1153, 1195, 1270, 1854
TaiI ACGT 2 cut(s) 2775, 3222
TaqI TCGA 5 cut(s) 831, 915, 1398, 1577, 3299
TatI WGTACW 1 cut(s) 1950
TauI GCSGC 1 cut(s) 863
TfiI GAWTC 7 cut(s) 203, 306, 599, 1076, 1121, 1718, 3271
TscAI CASTG 7 cut(s) 41, 431, 1040, 1191, 1965, 2660, 2953
TseFI GTSAC 1 cut(s) 1984
TseI GCWGC 5 cut(s) 580, 634, 1241, 2020, 3404
Tsp45I GTSAC 1 cut(s) 1984
TspGWI ACGGA 2 cut(s) 2560, 3245
TspRI CASTG 7 cut(s) 41, 431, 1040, 1191, 1965, 2660, 2953
Van91I CCANNNNNTGG 1 cut(s) 2125
Vha464I CTTAAG 1 cut(s) 3275
VpaK11BI GGWCC 3 cut(s) 421, 1149, 2315
XbaI TCTAGA 2 cut(s) 1727, 2287
XceI RCATGY 3 cut(s) 713, 2612, 3482
XmiI GTMKAC 2 cut(s) 915, 1296
XmnI GAANNNNTTC 2 cut(s) 1502, 2181
ZrmI AGTACT 1 cut(s) 1952
Zsp2I ATGCAT 3 cut(s) 1307, 1551, 3019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.