Rorug05G0322400

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000085
Physical Location & Seq
Reverse (-)
38579447 .. 38586128
6682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0322400.1

Sequence Viewer

Length: 2883 bp
ATGGCTGAAGTTGTGGGTGGAGCTTTTCTCTCTTCTGCTCTTGCTGTTCTGTTTGATAGGATGGCTTCCCGTCCGGTTCTTGACTTCATCGGAGGAAAGAAGACCACCGCCGGGCTGCTCCACAAGTTGAAGATGAAGTTGAAGTTGGTTAATAACTTTCTCGATGATGCAGAGGAGAAGCAACTCAGAAAATCTGGAGTCAGGGAATGGCTTAATGAGCTTAAAGATGTTCACTATCTTGCCGATGACCTGTTGGATGACATCGAGACTGAAGCTCTGCGTTGCAAGATGGAACAAGAAGATTCTGGAAGCAGCAGCATAAATCAGGTGTTAAGGAGACTCAAGTTTAGTTCTATTTCCATTCATAAATTTGACAAAAGTCTGGAACCCAGGATACGGTATATTCTTGAAAGACTGCAATCTGTTGTCGACGGAAAAGATGTGCTTGATTTGAAAGAAGGTGTTAAGGAGAGATCACAAGAAAGACTGCCTACAACTTCTTTGGTAGAAGAGTCTAGCACTGTATATGGAAGGGATGAAGAAAAGGAGGCCATTATCAAGTTATTGTTAGCGGATGGAATGACTGGAACTAAGACAGAAGTGCTTCCAATTGTGGGCATGGGTGGGCTTGGAAAGACCACTCTTACTCAACTCGTATACAACGATGACAGAGTCGAGCAACATTTTAACCATCGGTCATGGTTTTGTGTTTCTGAAGGATTTGATGTTACCAAAATAACACAGTACATTTACGGGTCGGTGACTTCAGCAGTTTGTAATATCACAGACCTAGACATTCTTCAAGTTAAACTAAAGGAGATGTTGTCAGGGAAAAAATTTCTCTTTGTTCTTGATGATGTTTGGACTGTGACTAGTGAGCAGTGGGGTCTCTTAAGCCTACCCTTTGAGAGTGGAGATCATGGAAGTAAGATCATTGTCACAACACGTGATGAAGGTGTCGCAGACCAGATGGATAAAGGTACCGCATGCAGGATAGGTACCCTTCCAAGTAAACGTCTAGTGCAACTATCTGAGGAAAACTGCTGGTTGTTGTTCTCAAAACATGCCTTCAAGAATGCAGGTGTTACTGCAGATCCATCGCTTGAATTGATTGGAAGACAGATTTCTCAAAAGTGTAAAGGACTCCCGTTAGCTGCAAAATCACTTGGGGGTCTTTTACGTTCTGAACCAAATGTTGAGAAATGGGAAGAAGTATTGAACACTGACATATGGGAGTTGCTAGATAAAAAGAATGGCATTTTGCCAGCTCTATGGTTGAGCTACCATAATCTACCTCCGCATCTGAAGCGTTGTTTTGCTTACTGTTCCATATTTCCTCAAGATTATGAGTTTCACAAACCGGATCTGGTTTTGTTGTGGATGGCTGAAGATCTCTTACCGTCGAATTCGAAGACTACATTTGAACAAGTTGGAGAGAACTACTTTGATGATCTTGTGTCAAGGTCATTTTTTCAACATGTACCGGATGAATTTTCTGGTAACGCACTTTTCACAATGCATGACCTTATCCATGACTTGGCAAACTTTGTCTCTGGCGAGTTTTGTGTTAGGTTTGAGAACAATGACTCAGTACTGGCAAATGTCAGCAAGAGTCGCCACTTTTCGTGTCGAGAAACTGTCTGGTTTGATCAATGGCACAGTTTGTATGAAGCCAAGCATTTGAGGACCTTGATAATGTCAAATTGTTACCTCATGAAAAAATTTGATATATTCCTGAAGCTTCGGTGTTTAAGAGTGCTCAATTTATCAAGTTACAATATTGGTGAGCTGCCTAGTTCAATTAGCAAGTTGAAGCATCTGAGGCACTTTGACTTGTCTCATTCATCAATTAAAAAGTTACCTGATGCAATGTGTACTTTATATAATTTACAGACGTTATTGTTGCGGTTTTGTAGAAATCTAGTTAAGTTGCCAACTGATTTTGGAAGATTAATCAACTTGAGTCATCTTGATATTGAAGGCACATATATAAAAAAGATGCCACCAAGGATGGGCAAAATGAAAGATCTCCAGACATTAAGAGGTCCGTTTGTCCTAGACAAATACACTGGTGTTAACATTGTCGAGATTAAAGAGCTTCAGCAGTTGCGCGGAACACTTCATATCTCGGGGCTTCGCAATATTGTGCATGTCAAAGATGCCTTGGAGACCAATATCAGGAAGAAGAAGTATCTGAATGAATTAGTTTTGGGATGGGGAGGCAAGTCTGGTGACACCGACGATAAAGAAAAAGAGAGAGATGTGCTGGAGAACCTCCAACCTCATACAAATCTAAAAGAACTCACCATTCGATCTTACGGGGGCACAAGATTTCCAGGTTGGTTGGAAGATCATTCTAGTTTATCTAATCTGAATATGCAAAAGAGAAAATCAGGTGACAAGTCGTCACAGAAGGCTGCTATTAAGGCTTCCAAAAAATTAAAGAATTCTGATGCTGCACATCAGGAATTGGAATCATTTCCTGAAGGGGGCTTGCCGTCTAAGTTAGAATACTTGAGCATCTGGCGTTGCAAGAAACTCCTCATTGGAAACCGTATGGAGTGGGGTCTACCAACACTCATCTTTCTCAGATTCTTGGGTGTCGACTTTGAGGGATGTGAACAAGTAGGAGATTCATTTCCAGACAAGGGGCTGCTGCCCACCACTCTCGGATCACCCGACAAGGGGTTTAAACAACTCGCCTCTCTTAACCAGTTGATAATTCGCGATTGCCCTGAACTCCGGTGCTTGCCAGATGAAGGGCTACCCACTTCTCTTTCTCATCTGGACATCGATCATTGTCCTTTACTAGAACAACGTTGCCAGAGAGAGACGGGAAGATTGGCCCAAGATTGCTCACATCAAGAGCATTTATATCAATAA

Protein Analysis

960

Amino Acids

109.17

Weight (kDa)

6.76

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 9 - 101 1.2e-16 Rx N-terminal domain
NB-ARC PF00931 180 - 359 6.3e-34 NB-ARC domain
WHD_DRP PF23559 444 - 514 3e-25 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 636 2.7e-11 Leucine-rich repeat region
LRR_8 PF13855 584 - 638 2.3e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 694 - 792 1.6e-28 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1070
Acc36I ACCTGC 1 cut(s) 1070
Acc65I GGTACC 2 cut(s) 980, 998
AccB1I GGYRCC 2 cut(s) 980, 998
AccB7I CCANNNNNTGG 1 cut(s) 1537
AccI GTMKAC 4 cut(s) 429, 657, 2569, 2604
AccII CGCG 2 cut(s) 2112, 2727
AciI CCGC 6 cut(s) 108, 572, 984, 1298, 1906, 2112
AclI AACGTT 1 cut(s) 2818
AclWI GGATC 3 cut(s) 1088, 1371, 2680
AcsI RAATTY 6 cut(s) 368, 836, 1405, 1490, 1721, 2446
AcuI CTGAAG 9 cut(s) 27, 291, 735, 750, 1325, 1407, 1757, 2084, 2505
AcvI CACGTG 1 cut(s) 947
AfaI GTAC 6 cut(s) 746, 982, 1000, 1482, 1593, 1876
AflII CTTAAG 1 cut(s) 892
AflIII ACRYGT 2 cut(s) 944, 1477
AhdI GACNNNNNGTC 1 cut(s) 2404
AhlI ACTAGT 1 cut(s) 872
AjnI CCWGG 2 cut(s) 389, 2335
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 9 cut(s) 23, 220, 275, 1154, 1268, 1281, 1741, 1789, 2098
AluI AGCT 9 cut(s) 23, 220, 275, 1154, 1268, 1281, 1741, 1789, 2098
Alw21I GWGCWC 1 cut(s) 1761
Alw26I GTCTC 7 cut(s) 260, 331, 893, 1555, 1842, 2162, 2825
AlwI GGATC 3 cut(s) 1088, 1371, 2680
Ama87I CYCGRG 1 cut(s) 2128
AoxI GGCC 2 cut(s) 549, 2844
ApeKI GCWGC 9 cut(s) 115, 312, 315, 1154, 1789, 2417, 2456, 2653, 2656
ApoI RAATTY 6 cut(s) 368, 836, 1405, 1490, 1721, 2446
AseI ATTAAT 1 cut(s) 1952
Asp700I GAANNNNTTC 2 cut(s) 603, 2478
Asp718I GGTACC 2 cut(s) 980, 998
AspLEI GCGC 1 cut(s) 2112
AspS9I GGNCC 3 cut(s) 1686, 2045, 2845
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 6 cut(s) 772, 1796, 2243, 2296, 2408, 2667
AsuII TTCGAA 1 cut(s) 1409
AvaI CYCGRG 1 cut(s) 2128
AvaII GGWCC 2 cut(s) 1686, 2045
BaeGI GKGCMC 1 cut(s) 2327
BanI GGYRCC 2 cut(s) 980, 998
BarI GAAGNNNNNNTAC 2 cut(s) 1380, 1412
BbrPI CACGTG 1 cut(s) 947
BbsI GAAGAC 3 cut(s) 107, 1123, 1418
Bbv12I GWGCWC 1 cut(s) 1761
BbvI GCAGC 9 cut(s) 102, 324, 327, 1141, 1776, 2404, 2443, 2640, 2643
BccI CCATC 9 cut(s) 55, 283, 569, 699, 964, 1105, 1375, 2005, 2208
BceAI ACGGC 1 cut(s) 2482
BcgI CGANNNNNNTGC 2 cut(s) 1080, 1114
BciT130I CCWGG 2 cut(s) 391, 2337
BciVI GTATCC 1 cut(s) 387
BclI TGATCA 1 cut(s) 1648
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 7 cut(s) 260, 331, 893, 1555, 1842, 2162, 2825
BcuI ACTAGT 1 cut(s) 872
BfmI CTRYAG 1 cut(s) 1089
BfrI CTTAAG 1 cut(s) 892
BfuAI ACCTGC 1 cut(s) 1070
BfuI GTATCC 1 cut(s) 387
BglII AGATCT 2 cut(s) 1390, 2026
BisI GCNGC 9 cut(s) 116, 313, 316, 1155, 1790, 2418, 2457, 2654, 2657
BlsI GCNGC 9 cut(s) 117, 314, 317, 1156, 1791, 2419, 2458, 2655, 2658
BmcAI AGTACT 1 cut(s) 1593
Bme1390I CCNGG 3 cut(s) 112, 391, 2337
Bme18I GGWCC 2 cut(s) 1686, 2045
BmeRI GACNNNNNGTC 1 cut(s) 2404
BmeT110I CYCGRG 1 cut(s) 2128
BmgT120I GGNCC 3 cut(s) 1686, 2045, 2845
BmiI GGNNCC 3 cut(s) 387, 982, 1000
BmrFI CCNGG 3 cut(s) 112, 391, 2337
BmsI GCATC 8 cut(s) 157, 1309, 1825, 1855, 1989, 2149, 2443, 2529
BoxI GACNNNNGTC 1 cut(s) 378
BpiI GAAGAC 3 cut(s) 107, 1123, 1418
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpmI CTGGAG 3 cut(s) 216, 2015, 2288
Bpu14I TTCGAA 1 cut(s) 1409
BpuEI CTTGAG 4 cut(s) 326, 1323, 1981, 2536
BpuMI CCSGG 1 cut(s) 112
Bsa29I ATCGAT 1 cut(s) 2793
BsaAI YACGTR 1 cut(s) 947
BsaI GGTCTC 2 cut(s) 893, 2162
BsaJI CCNNGG 3 cut(s) 389, 2006, 2163
BsaWI WCCGGW 4 cut(s) 73, 1360, 1483, 2742
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bse1I ACTGG 4 cut(s) 589, 1599, 2074, 2713
Bse3DI GCAATG 1 cut(s) 1875
BseBI CCWGG 2 cut(s) 391, 2337
BseCI ATCGAT 1 cut(s) 2793
BseDI CCNNGG 3 cut(s) 389, 2006, 2163
BseGI GGATG 9 cut(s) 66, 262, 541, 580, 1386, 1492, 2016, 2219, 2621
BseMI GCAATG 1 cut(s) 1875
BseMII CTCAG 5 cut(s) 199, 1023, 1602, 1811, 2602
BseNI ACTGG 4 cut(s) 589, 1599, 2074, 2713
BseRI GAGGAG 2 cut(s) 188, 2531
BseSI GKGCMC 1 cut(s) 2327
BseXI GCAGC 9 cut(s) 102, 324, 327, 1141, 1776, 2404, 2443, 2640, 2643
BsgI GTGCAG 1 cut(s) 2442
Bsh1236I CGCG 2 cut(s) 2112, 2727
BshFI GGCC 2 cut(s) 551, 2846
BshNI GGYRCC 2 cut(s) 980, 998
BshVI ATCGAT 1 cut(s) 2793
BsiHKAI GWGCWC 1 cut(s) 1761
BsiHKCI CYCGRG 1 cut(s) 2128
BsiSI CCGG 5 cut(s) 74, 111, 1361, 1484, 2743
BsmAI GTCTC 7 cut(s) 260, 331, 893, 1555, 1842, 2162, 2825
BsmBI CGTCTC 1 cut(s) 2825
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 2 cut(s) 551, 2846
Bso31I GGTCTC 2 cut(s) 893, 2162
BsoBI CYCGRG 1 cut(s) 2128
Bsp119I TTCGAA 1 cut(s) 1409
Bsp1286I GDGCHC 2 cut(s) 1761, 2327
Bsp68I TCGCGA 1 cut(s) 2727
BspACI CCGC 6 cut(s) 108, 572, 984, 1298, 1906, 2112
BspANI GGCC 2 cut(s) 551, 2846
BspCNI CTCAG 5 cut(s) 198, 1024, 1601, 1812, 2601
BspDI ATCGAT 1 cut(s) 2793
BspFNI CGCG 2 cut(s) 2112, 2727
BspHI TCATGA 1 cut(s) 1713
BspLI GGNNCC 3 cut(s) 387, 982, 1000
BspMAI CTGCAG 1 cut(s) 1093
BspMI ACCTGC 1 cut(s) 1070
BspPI GGATC 3 cut(s) 1088, 1371, 2680
BspT104I TTCGAA 1 cut(s) 1409
BspT107I GGYRCC 2 cut(s) 980, 998
BspTI CTTAAG 1 cut(s) 892
BspTNI GGTCTC 2 cut(s) 893, 2162
BsrDI GCAATG 1 cut(s) 1875
BsrI ACTGG 4 cut(s) 589, 1599, 2074, 2713
BssECI CCNNGG 3 cut(s) 389, 2006, 2163
BssNAI GTATAC 1 cut(s) 658
BssT1I CCWWGG 2 cut(s) 2006, 2163
Bst1107I GTATAC 1 cut(s) 658
Bst2UI CCWGG 2 cut(s) 391, 2337
Bst4CI ACNGT 9 cut(s) 399, 523, 744, 868, 1325, 1401, 1639, 1661, 2555
Bst6I CTCTTC 2 cut(s) 37, 504
BstAFI CTTAAG 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 947
BstBI TTCGAA 1 cut(s) 1409
BstC8I GCNNGC 4 cut(s) 988, 1266, 2495, 2750
BstDEI CTNAG 7 cut(s) 185, 591, 1032, 1588, 1820, 2502, 2588
BstF5I GGATG 9 cut(s) 66, 262, 541, 580, 1386, 1492, 2016, 2219, 2621
BstFNI CGCG 2 cut(s) 2112, 2727
BstHHI GCGC 1 cut(s) 2112
BstMAI GTCTC 7 cut(s) 260, 331, 893, 1555, 1842, 2162, 2825
BstMWI GCNNNNNNNGC 5 cut(s) 217, 1306, 1614, 1822, 2426
BstNI CCWGG 2 cut(s) 391, 2337
BstNSI RCATGY 4 cut(s) 990, 1067, 1481, 2153
BstPAI GACNNNNGTC 1 cut(s) 378
BstSCI CCNGG 3 cut(s) 110, 389, 2335
BstSFI CTRYAG 1 cut(s) 1089
BstSLI GKGCMC 1 cut(s) 2327
BstUI CGCG 2 cut(s) 2112, 2727
BstV1I GCAGC 9 cut(s) 102, 324, 327, 1141, 1776, 2404, 2443, 2640, 2643
BstV2I GAAGAC 3 cut(s) 107, 1123, 1418
BstX2I RGATCY 4 cut(s) 1093, 1363, 1390, 2026
BstXI CCANNNNNNTGG 1 cut(s) 1272
BstYI RGATCY 4 cut(s) 1093, 1363, 1390, 2026
BstZ17I GTATAC 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 2793
BsuI GTATCC 1 cut(s) 387
BsuRI GGCC 2 cut(s) 551, 2846
BsuTUI ATCGAT 1 cut(s) 2793
BtgZI GCGATG 1 cut(s) 1083
BtsCI GGATG 9 cut(s) 66, 262, 541, 580, 1386, 1492, 2016, 2219, 2621
BtsI GCAGTG 1 cut(s) 887
BtsIMutI CAGTG 4 cut(s) 519, 887, 1221, 2067
BtuMI TCGCGA 1 cut(s) 2727
BveI ACCTGC 1 cut(s) 1070
Cac8I GCNNGC 4 cut(s) 988, 1266, 2495, 2750
CciI TCATGA 1 cut(s) 1713
CfoI GCGC 1 cut(s) 2112
Cfr13I GGNCC 3 cut(s) 1686, 2045, 2845
ClaI ATCGAT 1 cut(s) 2793
Csp6I GTAC 6 cut(s) 745, 981, 999, 1481, 1592, 1875
CviQI GTAC 6 cut(s) 745, 981, 999, 1481, 1592, 1875
DdeI CTNAG 7 cut(s) 185, 591, 1032, 1588, 1820, 2502, 2588
DraI TTTAAA 1 cut(s) 2692
DriI GACNNNNNGTC 1 cut(s) 2404
Eam1104I CTCTTC 2 cut(s) 37, 504
Eam1105I GACNNNNNGTC 1 cut(s) 2404
EarI CTCTTC 2 cut(s) 37, 504
Eco130I CCWWGG 2 cut(s) 2006, 2163
Eco31I GGTCTC 2 cut(s) 893, 2162
Eco47I GGWCC 2 cut(s) 1686, 2045
Eco57I CTGAAG 9 cut(s) 27, 291, 735, 750, 1325, 1407, 1757, 2084, 2505
Eco72I CACGTG 1 cut(s) 947
Eco88I CYCGRG 1 cut(s) 2128
EcoO109I RGGNCCY 1 cut(s) 1686
EcoRI GAATTC 2 cut(s) 1405, 2446
EcoRII CCWGG 2 cut(s) 389, 2335
EcoT14I CCWWGG 2 cut(s) 2006, 2163
EcoT22I ATGCAT 1 cut(s) 1521
ErhI CCWWGG 2 cut(s) 2006, 2163
Esp3I CGTCTC 1 cut(s) 2825
FalI AAGNNNNNCTT 2 cut(s) 429, 461
FauNDI CATATG 1 cut(s) 1229
FbaI TGATCA 1 cut(s) 1648
FblI GTMKAC 4 cut(s) 429, 657, 2569, 2604
Fnu4HI GCNGC 9 cut(s) 116, 313, 316, 1155, 1790, 2418, 2457, 2654, 2657
FokI GGATG 9 cut(s) 73, 269, 548, 587, 1393, 1499, 2023, 2226, 2628
Fsp4HI GCNGC 9 cut(s) 116, 313, 316, 1155, 1790, 2418, 2457, 2654, 2657
GlaI GCGC 1 cut(s) 2111
GluI GCNGC 9 cut(s) 116, 313, 316, 1155, 1790, 2418, 2457, 2654, 2657
GsuI CTGGAG 3 cut(s) 216, 2015, 2288
HaeIII GGCC 2 cut(s) 551, 2846
HapII CCGG 5 cut(s) 74, 111, 1361, 1484, 2743
HhaI GCGC 1 cut(s) 2112
Hin6I GCGC 1 cut(s) 2110
HinP1I GCGC 1 cut(s) 2110
HincII GTYRAC 3 cut(s) 430, 2077, 2605
HindII GTYRAC 3 cut(s) 430, 2077, 2605
HindIII AAGCTT 1 cut(s) 1739
HpaI GTTAAC 1 cut(s) 2077
HpaII CCGG 5 cut(s) 74, 111, 1361, 1484, 2743
HphI GGTGA 6 cut(s) 772, 1796, 2243, 2296, 2408, 2667
Hpy166II GTNNAC 9 cut(s) 232, 430, 658, 1013, 1875, 2077, 2570, 2605, 2621
Hpy8I GTNNAC 9 cut(s) 232, 430, 658, 1013, 1875, 2077, 2570, 2605, 2621
Hpy99I CGWCG 3 cut(s) 434, 1405, 2243
HpyCH4III ACNGT 9 cut(s) 399, 523, 744, 868, 1325, 1401, 1639, 1661, 2555
HpyCH4IV ACGT 5 cut(s) 946, 1015, 1180, 1895, 2818
HpyF10VI GCNNNNNNNGC 5 cut(s) 217, 1306, 1614, 1822, 2426
HpyF3I CTNAG 7 cut(s) 185, 591, 1032, 1588, 1820, 2502, 2588
HpySE526I ACGT 5 cut(s) 946, 1015, 1180, 1895, 2818
HspAI GCGC 1 cut(s) 2110
KpnI GGTACC 2 cut(s) 984, 1002
Ksp22I TGATCA 1 cut(s) 1648
KspAI GTTAAC 1 cut(s) 2077
LmnI GCTCC 2 cut(s) 20, 123
Lsp1109I GCAGC 9 cut(s) 102, 324, 327, 1141, 1776, 2404, 2443, 2640, 2643
LweI GCATC 8 cut(s) 157, 1309, 1825, 1855, 1989, 2149, 2443, 2529
MaeII ACGT 5 cut(s) 946, 1015, 1180, 1895, 2818
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 4 cut(s) 1093, 1363, 1390, 2026
MhlI GDGCHC 2 cut(s) 1761, 2327
MlyI GAGTC 8 cut(s) 207, 333, 521, 681, 1137, 1580, 1621, 1972
MmeI TCCRAC 4 cut(s) 234, 1411, 2302, 2325
Mph1103I ATGCAT 1 cut(s) 1521
MroXI GAANNNNTTC 2 cut(s) 603, 2478
MspCI CTTAAG 1 cut(s) 892
MspI CCGG 5 cut(s) 74, 111, 1361, 1484, 2743
MspR9I CCNGG 3 cut(s) 112, 391, 2337
MssI GTTTAAAC 1 cut(s) 2692
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 1 cut(s) 1081
MvaI CCWGG 2 cut(s) 391, 2337
MvnI CGCG 2 cut(s) 2112, 2727
MwoI GCNNNNNNNGC 5 cut(s) 217, 1306, 1614, 1822, 2426
NciI CCSGG 1 cut(s) 112
NdeI CATATG 1 cut(s) 1229
NlaIV GGNNCC 3 cut(s) 387, 982, 1000
NmuCI GTSAC 6 cut(s) 760, 868, 937, 2231, 2396, 2406
NruI TCGCGA 1 cut(s) 2727
NsiI ATGCAT 1 cut(s) 1521
NspI RCATGY 4 cut(s) 990, 1067, 1481, 2153
NspV TTCGAA 1 cut(s) 1409
PaeI GCATGC 1 cut(s) 990
PagI TCATGA 1 cut(s) 1713
PaqCI CACCTGC 1 cut(s) 1070
PciI ACATGT 1 cut(s) 1477
PcsI WCGNNNNNNNCGW 2 cut(s) 660, 2676
PctI GAATGC 1 cut(s) 1081
PdmI GAANNNNTTC 2 cut(s) 603, 2478
PfeI GAWTC 4 cut(s) 302, 2474, 2592, 2633
PflFI GACNNNGTC 1 cut(s) 671
PflMI CCANNNNNTGG 1 cut(s) 1537
PkrI GCNGC 9 cut(s) 117, 314, 317, 1156, 1791, 2419, 2458, 2655, 2658
PleI GAGTC 8 cut(s) 206, 333, 520, 680, 1137, 1580, 1620, 1971
PmaCI CACGTG 1 cut(s) 947
PmeI GTTTAAAC 1 cut(s) 2692
PmlI CACGTG 1 cut(s) 947
PpsI GAGTC 8 cut(s) 206, 333, 520, 680, 1137, 1580, 1620, 1971
Ppu21I YACGTR 1 cut(s) 947
PpuMI RGGWCCY 1 cut(s) 1686
PscI ACATGT 1 cut(s) 1477
PshAI GACNNNNGTC 1 cut(s) 378
PshBI ATTAAT 1 cut(s) 1952
Psp1406I AACGTT 1 cut(s) 2818
Psp5II RGGWCCY 1 cut(s) 1686
Psp6I CCWGG 2 cut(s) 389, 2335
PspCI CACGTG 1 cut(s) 947
PspGI CCWGG 2 cut(s) 389, 2335
PspN4I GGNNCC 3 cut(s) 387, 982, 1000
PspPI GGNCC 3 cut(s) 1686, 2045, 2845
PspPPI RGGWCCY 1 cut(s) 1686
PsrI GAACNNNNNNTAC 2 cut(s) 378, 410
PstI CTGCAG 1 cut(s) 1093
PsuI RGATCY 4 cut(s) 1093, 1363, 1390, 2026
PsyI GACNNNGTC 1 cut(s) 671
RruI TCGCGA 1 cut(s) 2727
RsaI GTAC 6 cut(s) 746, 982, 1000, 1482, 1593, 1876
RsaNI GTAC 6 cut(s) 745, 981, 999, 1481, 1592, 1875
SalI GTCGAC 2 cut(s) 428, 2603
SatI GCNGC 9 cut(s) 116, 313, 316, 1155, 1790, 2418, 2457, 2654, 2657
Sau96I GGNCC 3 cut(s) 1686, 2045, 2845
ScaI AGTACT 1 cut(s) 1593
SchI GAGTC 8 cut(s) 207, 333, 521, 681, 1137, 1580, 1621, 1972
ScrFI CCNGG 3 cut(s) 112, 391, 2337
SduI GDGCHC 2 cut(s) 1761, 2327
SfaNI GCATC 8 cut(s) 157, 1309, 1825, 1855, 1989, 2149, 2443, 2529
SfcI CTRYAG 1 cut(s) 1089
SfuI TTCGAA 1 cut(s) 1409
SinI GGWCC 2 cut(s) 1686, 2045
SmlI CTYRAG 5 cut(s) 341, 892, 1338, 1960, 2515
SmoI CTYRAG 5 cut(s) 341, 892, 1338, 1960, 2515
SpeI ACTAGT 1 cut(s) 872
SphI GCATGC 1 cut(s) 990
SsiI CCGC 6 cut(s) 108, 572, 984, 1298, 1906, 2112
SspI AATATT 2 cut(s) 1780, 2143
StyD4I CCNGG 3 cut(s) 110, 389, 2335
StyI CCWWGG 2 cut(s) 2006, 2163
TaaI ACNGT 9 cut(s) 399, 523, 744, 868, 1325, 1401, 1639, 1661, 2555
TaiI ACGT 5 cut(s) 949, 1018, 1183, 1898, 2821
TaqII GACCGA 1 cut(s) 684
TatI WGTACW 3 cut(s) 744, 1591, 1874
TfiI GAWTC 4 cut(s) 302, 2474, 2592, 2633
TscAI CASTG 4 cut(s) 526, 887, 1228, 2074
TseFI GTSAC 6 cut(s) 760, 868, 937, 2231, 2396, 2406
TseI GCWGC 9 cut(s) 115, 312, 315, 1154, 1789, 2417, 2456, 2653, 2656
Tsp45I GTSAC 6 cut(s) 760, 868, 937, 2231, 2396, 2406
TspGWI ACGGA 2 cut(s) 447, 2037
TspRI CASTG 4 cut(s) 526, 887, 1228, 2074
Tth111I GACNNNGTC 1 cut(s) 671
Van91I CCANNNNNTGG 1 cut(s) 1537
Vha464I CTTAAG 1 cut(s) 892
VpaK11BI GGWCC 2 cut(s) 1686, 2045
VspI ATTAAT 1 cut(s) 1952
XapI RAATTY 6 cut(s) 368, 836, 1405, 1490, 1721, 2446
XceI RCATGY 4 cut(s) 990, 1067, 1481, 2153
XmiI GTMKAC 4 cut(s) 429, 657, 2569, 2604
XmnI GAANNNNTTC 2 cut(s) 603, 2478
ZrmI AGTACT 1 cut(s) 1593
Zsp2I ATGCAT 1 cut(s) 1521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.