RLG00000036825

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000085
Physical Location & Seq
Reverse (-)
85246927 .. 85251916
4990 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036825

Sequence Viewer

Length: 4026 bp
ATGCTAGTTTTTGAATCTCTTCCTTCATCAAATGAAGCATCATCAACGGCTCGTCGCTGGAAGGATGATGTGTTTTTGAGTTACAAGAGTGGTGACACTCCAAAGGGTTTTATATTCCATTTATACGACCGGCTGCAACAGAGGAAAATGACAACCTTCATGGATCATTCAGATTTTCAAGTAGGGGATGCTATCTCTCCCTCTCTCCTAAAGGCAATTGAAGAATCAAAGTTCGCAGTTGTTGTTCTGTCCCAAAATTATGCTTCTTCTACTTGTTGTTTGGAGGAACTTACAAAGATTTGTCAGTTCACAGAGGACGGCTTTACAATTCTTCCAGTTTTTTATGAGTTCGACCCTATTAATGTAAGAAATCAAAAGGGGAGTTTTCAGGATGCTTTCGCCAAGCACGAAAGGTCTGGGCGACACCAACCGCAGAAGGTCCAGCAATGGAGAGATGCTTTAACGAAAGTGGGCTGTTTGTCTGGGTTGAATACACAGGATTACAGGACTGACAATGATCTTGTCCAAGCCATTATTGAATCTATGTGTAGTCAAATGAAGACTATTGAACTTGTGCATACAATTTCGGAGTCAGATGAAGATTGCATCGTGGAATCGGAGTCAGATGAAGATTCGACTGTGGAACCTGTGTGCAGTCAAATGGAGACTATTGAACTTAAGCCCACAATGCCAAAGGATTTTGAAGCAGTTGAAGCAACAACACAAGCCACCAATGGAAACACTGAACCAGCTCCTCAGTGGAAGCATGATGTGTTTTTGAGCTTTAGGGGTGATGACACTCGCAAGGGTTTTCTATCCCATTTATACCATGAATTACAAAACACGAAAGTAATTAGAACATTTAAGGATGATGAACAACTTAAGAAAGGGAAGGATATTTCTCAAAGTCTGCTGACTGCAATTGAAGAATCAAGGTTTGCGATCATCGTTCTGTCAGAAGACTATGCTTCTTCTGCATGGTGTTTAGATGAACTTTCAAAGATTTTTCAGTGCATGGAAGGCAAGGATACAATTCTGCCAATTTTTTATCATGTGGATCCCTCTGATGTACGATATCAAAACAATAAACTCGAAACGGCCTTTACAGAGCATTCAAGCAAGGAAAGGTATGACATAGAGAAGATGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGAAATCTCTCTGGGTGGGATTCAAAGAACCACAAATCTGAAAGGGAACTCGTTGAAGACATAGTGAAATTTGTGCTCAAAGAAGTACTTCAAGAACCAAGGGGAGATTTTGAAGAATTTGAAGCAACAACAGAAGCCATGGATGAGGTTAAAAAGATGCTGAAAGATGATAAGATCACCGCCATTGGGGTCTATGGAAGAGGAGGCGTTGGCAAGACAGCCATGGTGAAACATGTCGGTGCACAAGCCAAAAGAATTGGGCTTTTTGATCATGTTGTTATGGCTGTTATATCCCGAAGCCCTGATTTGCAAAAAATTCAAGGCGCATTGGTAAATCAGCTGGGATTGGAATTGCTGGAGAATGAACAAACAGAAAGAGCCAGTAGATTGAAGGAGAGGATCATGGGAGGAAAGAGGATCCTTATTATCTTGGACGACATTTGGGAGATGATAGATTTGTCAAGCATTGGAATTCCTAGCTATGACGAGCTTCAAAGATGCGATGCCAAGGTCCTACTCACCACAAGGATCAGGAATCTTTGTCATTCTATGGGATGCCAAGCACATATTCCTCTCAATATCCTATCAGAGCAAGATTCTTTGAAGTTATTCATGAAGAAGTCGAGAAAGCCTTTTCATGAATCTACTGACTCCTATGATGAAGAGAGGAACATAGTTAGAGAATGCGCTGGTCTCCCAAATGTATTGGAAGAAGCTGCAAGGGCACTTGGACATAAATACGTGGAAGAATGGAACGAAGCAGTTTCAAAGGCATTTGGAGATGATGACGATGAATTGCAAAAAGCAGCTCAAGAACTAAATGCATCTCAACTTGTCCACACTCAAGATGAGAGAGATACATTTAGAAGAATAAAGTTAAGCTATCATACCTTGAGGTTTGATGATGCCAAGTCATTTTTCTTGCTTTGCTGCATGTTTCCAGAAGATTATGATATCCCAATTGAAGAACTTTTGAAGTATGGAATTGGAAAAGGTTTGCTTCAATATTCAGACACATTGCAAGAAGCTAGAGACAGAGCACATTCTTTGGTCGCATACCTTAAAGCTTCGGGGTTGCTTTTGGATGGCAAGAAGGGATGCATAAGGATGCATTGTGACATCCGAAATGTGGCCATGTCTATTGCATTGTCTGAAGAAGGTCAGGCTATTTACGTAAAAGCTGGTTGTGAATTGAAGGATTGGCCAATGGATGCTCTTGAGGGCTACTCAGCAATCTCACTAATGAGGAACAAACTTAACAAGCTTCCCGAAACTTTGGTATGTTCTGAACTCCAAATCTTACTGCTGCAAAGTAATGCTGGTATATGTGACATTCCAAAAACTTTTTTTCAAAGTCCAAATGCACTAAGGGTCATTGATCTCAGCTGTACCAGTATTTCAGTACTACCCTCTTCATTCAATGTCCTCACCGACCTCCAAGCCTTGTTTTTGGATTGTTGCAATAAAATAACTGACATTTCCGTACTTAGAAAGCTGAAGAAAGTTGAGATTCTCAGTTTGAGAAAATTTCCTCATAAGGAGTTGCCGAAAGAGATAGGAAATTTGACCAGTCTAAGGATGCTGGACGTCACTGGTGGATGTGTTCACAAAATTCCATCTGAAGTGATATCGAGGTTGTATAGACTCGAAGAATTGTACATGAAATGTGAATTTGGGAACTGGGGAAGTAAAGTCGAAGAAGCAGTAGGACAAACAAATGCGGACTTTGATGAGATAATTGGCTTGTCCCGTTTAAACATTTTGAAGGTTTACATATCTGATGCAGCTTGCTTACCCAAAGATATTGGGTACTTTGAGCCGAGTTGGGTAAAATTTGACATATGCATCAGCAGAGATCTCTCGTCCGAGATGATGCTTTCTCCGAATGATCAACCTTCAAGAACCTTGACTCTTGACAGAACAATTAATGGCTTATCGGATTGGTTTATCAATGTGGTGACGGAGAAAGCAATAAAACTAGAGTACAGAGAGTGTAAGGGATTAAAAAACATTCTTAAGGAATATGAGCATGGGAGATTACATGGGCTCAAGCATCTCTCTGTAATTGGCTGCCATGAGAACTTGGAAGAGCTGATAAATGTAACAACATGGGATCCAAGTAAACCTGTGTTCGAGAGCTTGGAAGTGTTGCATCTCCTTCACTTGCAAGGCCTCAAGGAGTTATGTGTTGGCGAATTACCACTTGGGTCTCTATGCAATCTCAAGTTATTGAAAGTGCAAACTTGTTGCAACTTGGTGAATGCACTTTTACCACCAAAATTGTTGCAGAGACTACCAAATCTAGAAACAATATTTTGTGTGAATATGGAACACATGAAATATGTGTTTGGATCTGAAGGGCTTGAGCCTGGAATGATTCTCTTGACAAAATTGAGCGAGATGAGACTGTGCGCTCTCTTTGAACTAATCAGCATCTGGAATGGTCCTGCTCCACATGCAGTCTTTCGTAATCTTAAAGTTTTGGCAGTCTGCATGTGTACAAAACTGAAATATCTATTCCCGTTTGATGTAGCTCGATCTCTTGTGCAATTGGAAGACCTCTCTGTAGAGCATTGCCTTAACTTGGGCAGAGTTATTGAAGCAAGCAACAGCTTGAACAGATATATCGTTTTTCTAGAATTGAAGAAATTAGTTTTGATAACTCTTCCAGAGCTCACATGGTTCTGCACCGAAAATGGTACTGTTGATATCGAGTGCCCTTTGTTGGAATACTTGTATGTGGATAAGTGCCCCCAGTTTCAAAACTCTGCTGCTGCTTCTGTTAAACTCAATGATCAACAACATTTGAATTCGTTAGTGAAAAGATGGGAGGATATATATAAGCTATAA

Protein Analysis

1342

Amino Acids

152.47

Weight (kDa)

5.4

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 187 1e-40 TIR domain
TIR_2 PF13676 24 - 119 4.4e-14 TIR domain
TIR PF01582 255 - 425 1.3e-49 TIR domain
TIR_2 PF13676 258 - 354 4.8e-15 TIR domain
NB-ARC PF00931 437 - 600 1.7e-28 NB-ARC domain
LRR_8 PF13855 822 - 878 3.9e-07 Leucine rich repeat
LRR_14 PF23598 884 - 947 1.6e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 1108 - 1251 2.4e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2766
AciI CCGC 3 cut(s) 431, 1356, 2897
AcoI YGGCCR 2 cut(s) 2307, 2378
AcuI CTGAAG 4 cut(s) 2349, 2693, 2817, 3552
AcyI GRCGYC 1 cut(s) 2763
AfiI CCNNNNNNNGG 5 cut(s) 1362, 2305, 2751, 3760, 3901
AflII CTTAAG 3 cut(s) 677, 881, 3191
AflIII ACRYGT 1 cut(s) 1408
AjnI CCWGG 1 cut(s) 3544
AjuI GAANNNNNNNTTGG 6 cut(s) 1728, 1760, 2078, 2110, 3363, 3395
Alw21I GWGCWC 4 cut(s) 1254, 1420, 2218, 3852
Alw26I GTCTC 6 cut(s) 659, 1874, 2202, 3390, 3460, 3574
Alw44I GTGCAC 1 cut(s) 1416
AlwNI CAGNNNCTG 1 cut(s) 3612
AoxI GGCC 4 cut(s) 1098, 2307, 2378, 3346
ApaLI GTGCAC 1 cut(s) 1416
ApeKI GCWGC 9 cut(s) 133, 1892, 1982, 2106, 2482, 2960, 3246, 3947, 3950
AseI ATTAAT 2 cut(s) 360, 3102
Asp700I GAANNNNTTC 3 cut(s) 18, 1263, 1784
AspLEI GCGC 3 cut(s) 1502, 1865, 3590
AspS9I GGNCC 3 cut(s) 439, 1687, 3620
AsuHPI GGTGA 8 cut(s) 104, 803, 1345, 1414, 1687, 2596, 3145, 3445
AvaII GGWCC 3 cut(s) 439, 1687, 3620
BaeGI GKGCMC 4 cut(s) 1420, 1903, 3896, 3929
BalI TGGCCA 2 cut(s) 2309, 2380
BamHI GGATCC 3 cut(s) 1057, 1593, 3289
BanII GRGCYC 2 cut(s) 3225, 3852
BbsI GAAGAC 4 cut(s) 566, 966, 1239, 3738
Bbv12I GWGCWC 4 cut(s) 1254, 1420, 2218, 3852
BbvI GCAGC 9 cut(s) 120, 1879, 1994, 2093, 2469, 2972, 3233, 3934, 3937
BccI CCATC 3 cut(s) 2255, 2800, 3996
BceAI ACGGC 3 cut(s) 63, 334, 1113
BciT130I CCWGG 1 cut(s) 3546
BciVI GTATCC 1 cut(s) 1021
BclI TGATCA 3 cut(s) 1444, 3064, 3970
BcoDI GTCTC 6 cut(s) 659, 1874, 2202, 3390, 3460, 3574
BfaI CTAG 6 cut(s) 5, 1653, 2205, 3155, 3479, 3812
BfmI CTRYAG 1 cut(s) 3741
BfrI CTTAAG 3 cut(s) 677, 881, 3191
BfuI GTATCC 1 cut(s) 1021
BglII AGATCT 1 cut(s) 3031
BisI GCNGC 9 cut(s) 134, 1893, 1983, 2107, 2483, 2961, 3247, 3948, 3951
BlsI GCNGC 9 cut(s) 135, 1894, 1984, 2108, 2484, 2962, 3248, 3949, 3952
BmcAI AGTACT 2 cut(s) 1263, 2580
Bme1390I CCNGG 1 cut(s) 3546
Bme18I GGWCC 3 cut(s) 439, 1687, 3620
BmgT120I GGNCC 3 cut(s) 439, 1687, 3620
BmiI GGNNCC 4 cut(s) 645, 1059, 1595, 3291
BmrFI CCNGG 1 cut(s) 3546
BmrI ACTGGG 2 cut(s) 2866, 3925
BmuI ACTGGG 2 cut(s) 2866, 3925
BpiI GAAGAC 4 cut(s) 566, 966, 1239, 3738
BplI GAGNNNNNCTC 2 cut(s) 2387, 2419
BpmI CTGGAG 1 cut(s) 1553
BpuEI CTTGAG 8 cut(s) 1971, 2004, 2089, 2414, 3209, 3335, 3383, 3560
BsaAI YACGTR 2 cut(s) 1918, 2350
BsaHI GRCGYC 1 cut(s) 2763
BsaI GGTCTC 2 cut(s) 1874, 3390
BsaJI CCNNGG 4 cut(s) 1274, 1314, 1398, 1683
Bsc4I CCNNNNNNNGG 5 cut(s) 1362, 2305, 2751, 3760, 3901
Bse118I RCCGGY 1 cut(s) 129
Bse1I ACTGG 7 cut(s) 335, 1557, 2568, 2745, 2773, 2861, 3931
Bse3DI GCAATG 3 cut(s) 452, 2192, 3748
BseBI CCWGG 1 cut(s) 3546
BseDI CCNNGG 4 cut(s) 1274, 1314, 1398, 1683
BseLI CCNNNNNNNGG 5 cut(s) 1362, 2305, 2751, 3760, 3901
BseMI GCAATG 3 cut(s) 452, 2192, 3748
BseMII CTCAG 4 cut(s) 770, 2418, 2572, 2704
BseNI ACTGG 7 cut(s) 335, 1557, 2568, 2745, 2773, 2861, 3931
BseRI GAGGAG 2 cut(s) 744, 1392
BseSI GKGCMC 4 cut(s) 1420, 1903, 3896, 3929
BseXI GCAGC 9 cut(s) 120, 1879, 1994, 2093, 2469, 2972, 3233, 3934, 3937
BseYI CCCAGC 1 cut(s) 1516
BsgI GTGCAG 2 cut(s) 673, 3847
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 4 cut(s) 1100, 2309, 2380, 3348
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 4 cut(s) 1254, 1420, 2218, 3852
BsiSI CCGG 1 cut(s) 130
BslFI GGGAC 2 cut(s) 235, 2908
BslI CCNNNNNNNGG 5 cut(s) 1362, 2305, 2751, 3760, 3901
BsmAI GTCTC 6 cut(s) 659, 1874, 2202, 3390, 3460, 3574
BsmFI GGGAC 2 cut(s) 235, 2908
BsmI GAATGC 3 cut(s) 1111, 1865, 3442
BsnI GGCC 4 cut(s) 1100, 2309, 2380, 3348
Bso31I GGTCTC 2 cut(s) 1874, 3390
Bsp1286I GDGCHC 8 cut(s) 1254, 1420, 1903, 2218, 3225, 3852, 3896, 3929
Bsp1407I TGTACA 2 cut(s) 2832, 3674
Bsp19I CCATGG 2 cut(s) 1314, 1398
BspACI CCGC 3 cut(s) 431, 1356, 2897
BspANI GGCC 4 cut(s) 1100, 2309, 2380, 3348
BspCNI CTCAG 4 cut(s) 769, 2417, 2571, 2703
BspHI TCATGA 2 cut(s) 1788, 1813
BspLI GGNNCC 4 cut(s) 645, 1059, 1595, 3291
BspQI GCTCTTC 1 cut(s) 3258
BspTI CTTAAG 3 cut(s) 677, 881, 3191
BspTNI GGTCTC 2 cut(s) 1874, 3390
BsrDI GCAATG 3 cut(s) 452, 2192, 3748
BsrFI RCCGGY 1 cut(s) 129
BsrGI TGTACA 2 cut(s) 2832, 3674
BsrI ACTGG 7 cut(s) 335, 1557, 2568, 2745, 2773, 2861, 3931
BssAI RCCGGY 1 cut(s) 129
BssECI CCNNGG 4 cut(s) 1274, 1314, 1398, 1683
BssNI GRCGYC 1 cut(s) 2763
BssT1I CCWWGG 4 cut(s) 1274, 1314, 1398, 1683
Bst2UI CCWGG 1 cut(s) 3546
Bst4CI ACNGT 3 cut(s) 640, 3585, 3880
Bst6I CTCTTC 6 cut(s) 24, 1369, 1833, 2593, 3258, 3846
BstACI GRCGYC 1 cut(s) 2763
BstAFI CTTAAG 3 cut(s) 677, 881, 3191
BstAUI TGTACA 2 cut(s) 2832, 3674
BstBAI YACGTR 2 cut(s) 1918, 2350
BstC8I GCNNGC 2 cut(s) 2965, 3781
BstDEI CTNAG 7 cut(s) 756, 2404, 2543, 2558, 2663, 2690, 2750
BstDSI CCRYGG 2 cut(s) 1314, 1398
BstHHI GCGC 3 cut(s) 1502, 1865, 3590
BstMAI GTCTC 6 cut(s) 659, 1874, 2202, 3390, 3460, 3574
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 6 cut(s) 688, 713, 974, 1020, 1898, 3632
BstNI CCWGG 1 cut(s) 3546
BstNSI RCATGY 4 cut(s) 1412, 2113, 3635, 3673
BstSCI CCNGG 1 cut(s) 3544
BstSFI CTRYAG 1 cut(s) 3741
BstSLI GKGCMC 4 cut(s) 1420, 1903, 3896, 3929
BstSNI TACGTA 1 cut(s) 2350
BstV1I GCAGC 9 cut(s) 120, 1879, 1994, 2093, 2469, 2972, 3233, 3934, 3937
BstV2I GAAGAC 4 cut(s) 566, 966, 1239, 3738
BstX2I RGATCY 5 cut(s) 1057, 1593, 3031, 3289, 3527
BstYI RGATCY 5 cut(s) 1057, 1593, 3031, 3289, 3527
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 4 cut(s) 1100, 2309, 2380, 3348
BtgI CCRYGG 2 cut(s) 1314, 1398
BtgZI GCGATG 1 cut(s) 1692
BtsI GCAGTG 1 cut(s) 1157
BtsIMutI CAGTG 5 cut(s) 741, 764, 1016, 1157, 2766
Cac8I GCNNGC 2 cut(s) 2965, 3781
CaiI CAGNNNCTG 1 cut(s) 3612
CciI TCATGA 2 cut(s) 1788, 1813
CfoI GCGC 3 cut(s) 1502, 1865, 3590
Cfr10I RCCGGY 1 cut(s) 129
Cfr13I GGNCC 3 cut(s) 439, 1687, 3620
CspCI CAANNNNNGTGG 2 cut(s) 3438, 3473
DdeI CTNAG 7 cut(s) 756, 2404, 2543, 2558, 2663, 2690, 2750
DraI TTTAAA 1 cut(s) 2931
EaeI YGGCCR 2 cut(s) 2307, 2378
Eam1104I CTCTTC 6 cut(s) 24, 1369, 1833, 2593, 3258, 3846
EarI CTCTTC 6 cut(s) 24, 1369, 1833, 2593, 3258, 3846
Ecl136II GAGCTC 1 cut(s) 3850
Eco105I TACGTA 1 cut(s) 2350
Eco130I CCWWGG 4 cut(s) 1274, 1314, 1398, 1683
Eco147I AGGCCT 1 cut(s) 3348
Eco24I GRGCYC 2 cut(s) 3225, 3852
Eco31I GGTCTC 2 cut(s) 1874, 3390
Eco32I GATATC 4 cut(s) 1076, 2131, 2805, 3886
Eco47I GGWCC 3 cut(s) 439, 1687, 3620
Eco53kI GAGCTC 1 cut(s) 3850
Eco57I CTGAAG 4 cut(s) 2349, 2693, 2817, 3552
EcoICRI GAGCTC 1 cut(s) 3850
EcoO109I RGGNCCY 1 cut(s) 1687
EcoRI GAATTC 2 cut(s) 1647, 3985
EcoRII CCWGG 1 cut(s) 3544
EcoRV GATATC 4 cut(s) 1076, 2131, 2805, 3886
EcoT14I CCWWGG 4 cut(s) 1274, 1314, 1398, 1683
EcoT22I ATGCAT 4 cut(s) 2002, 2279, 2289, 3023
EcoT38I GRGCYC 2 cut(s) 3225, 3852
ErhI CCWWGG 4 cut(s) 1274, 1314, 1398, 1683
FalI AAGNNNNNCTT 4 cut(s) 1248, 1280, 2160, 2192
FaqI GGGAC 2 cut(s) 235, 2908
FauNDI CATATG 1 cut(s) 3017
FbaI TGATCA 3 cut(s) 1444, 3064, 3970
Fnu4HI GCNGC 9 cut(s) 134, 1893, 1983, 2107, 2483, 2961, 3247, 3948, 3951
FriOI GRGCYC 2 cut(s) 3225, 3852
Fsp4HI GCNGC 9 cut(s) 134, 1893, 1983, 2107, 2483, 2961, 3247, 3948, 3951
FspBI CTAG 6 cut(s) 5, 1653, 2205, 3155, 3479, 3812
GlaI GCGC 3 cut(s) 1501, 1864, 3589
GluI GCNGC 9 cut(s) 134, 1893, 1983, 2107, 2483, 2961, 3247, 3948, 3951
GsaI CCCAGC 1 cut(s) 1520
GsuI CTGGAG 1 cut(s) 1553
HaeIII GGCC 4 cut(s) 1100, 2309, 2380, 3348
HapII CCGG 1 cut(s) 130
HhaI GCGC 3 cut(s) 1502, 1865, 3590
Hin1I GRCGYC 1 cut(s) 2763
Hin6I GCGC 3 cut(s) 1500, 1863, 3588
HinP1I GCGC 3 cut(s) 1500, 1863, 3588
HindIII AAGCTT 2 cut(s) 2241, 2438
HpaII CCGG 1 cut(s) 130
HphI GGTGA 8 cut(s) 104, 803, 1345, 1414, 1687, 2596, 3145, 3445
Hpy166II GTNNAC 7 cut(s) 309, 1418, 2014, 2782, 2947, 3299, 3675
Hpy8I GTNNAC 7 cut(s) 309, 1418, 2014, 2782, 2947, 3299, 3675
Hpy99I CGWCG 1 cut(s) 57
HpyCH4III ACNGT 3 cut(s) 640, 3585, 3880
HpyCH4IV ACGT 3 cut(s) 1917, 2349, 2763
HpyF10VI GCNNNNNNNGC 6 cut(s) 688, 713, 974, 1020, 1898, 3632
HpyF3I CTNAG 7 cut(s) 756, 2404, 2543, 2558, 2663, 2690, 2750
HpySE526I ACGT 3 cut(s) 1917, 2349, 2763
Hsp92I GRCGYC 1 cut(s) 2763
HspAI GCGC 3 cut(s) 1500, 1863, 3588
Ksp22I TGATCA 3 cut(s) 1444, 3064, 3970
LguI GCTCTTC 1 cut(s) 3258
LmnI GCTCC 2 cut(s) 757, 3631
Lsp1109I GCAGC 9 cut(s) 120, 1879, 1994, 2093, 2469, 2972, 3233, 3934, 3937
MaeI CTAG 6 cut(s) 5, 1653, 2205, 3155, 3479, 3812
MaeII ACGT 3 cut(s) 1917, 2349, 2763
MaeIII GTNAC 7 cut(s) 80, 92, 2291, 2504, 2764, 3133, 3277
MfeI CAATTG 4 cut(s) 216, 921, 2136, 3725
MflI RGATCY 5 cut(s) 1057, 1593, 3031, 3289, 3527
MhlI GDGCHC 8 cut(s) 1254, 1420, 1903, 2218, 3225, 3852, 3896, 3929
MlsI TGGCCA 2 cut(s) 2309, 2380
MluNI TGGCCA 2 cut(s) 2309, 2380
MlyI GAGTC 5 cut(s) 599, 629, 1820, 2814, 3079
MmeI TCCRAC 1 cut(s) 3882
Mox20I TGGCCA 2 cut(s) 2309, 2380
Mph1103I ATGCAT 4 cut(s) 2002, 2279, 2289, 3023
MroXI GAANNNNTTC 3 cut(s) 18, 1263, 1784
MscI TGGCCA 2 cut(s) 2309, 2380
MslI CAYNNNNRTG 2 cut(s) 1413, 2282
Msp20I TGGCCA 2 cut(s) 2309, 2380
MspA1I CMGCKG 2 cut(s) 1516, 2562
MspCI CTTAAG 3 cut(s) 677, 881, 3191
MspI CCGG 1 cut(s) 130
MspR9I CCNGG 1 cut(s) 3546
MssI GTTTAAAC 1 cut(s) 2931
MunI CAATTG 4 cut(s) 216, 921, 2136, 3725
Mva1269I GAATGC 3 cut(s) 1111, 1865, 3442
MvaI CCWGG 1 cut(s) 3546
MwoI GCNNNNNNNGC 6 cut(s) 688, 713, 974, 1020, 1898, 3632
NcoI CCATGG 2 cut(s) 1314, 1398
NdeI CATATG 1 cut(s) 3017
NlaIV GGNNCC 4 cut(s) 645, 1059, 1595, 3291
NmeAIII GCCGAG 1 cut(s) 3021
NmuCI GTSAC 5 cut(s) 92, 2291, 2504, 2764, 3133
NsiI ATGCAT 4 cut(s) 2002, 2279, 2289, 3023
NspI RCATGY 4 cut(s) 1412, 2113, 3635, 3673
PagI TCATGA 2 cut(s) 1788, 1813
PceI AGGCCT 1 cut(s) 3348
PciI ACATGT 1 cut(s) 1408
PciSI GCTCTTC 1 cut(s) 3258
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PctI GAATGC 3 cut(s) 1111, 1865, 3442
PdmI GAANNNNTTC 3 cut(s) 18, 1263, 1784
PkrI GCNGC 9 cut(s) 135, 1894, 1984, 2108, 2484, 2962, 3248, 3949, 3952
PleI GAGTC 5 cut(s) 598, 628, 1820, 2814, 3079
PmeI GTTTAAAC 1 cut(s) 2931
PpsI GAGTC 5 cut(s) 598, 628, 1820, 2814, 3079
Ppu21I YACGTR 2 cut(s) 1918, 2350
PpuMI RGGWCCY 1 cut(s) 1687
PscI ACATGT 1 cut(s) 1408
PshBI ATTAAT 2 cut(s) 360, 3102
Psp124BI GAGCTC 1 cut(s) 3852
Psp5II RGGWCCY 1 cut(s) 1687
Psp6I CCWGG 1 cut(s) 3544
PspFI CCCAGC 1 cut(s) 1516
PspGI CCWGG 1 cut(s) 3544
PspN4I GGNNCC 4 cut(s) 645, 1059, 1595, 3291
PspPI GGNCC 3 cut(s) 439, 1687, 3620
PspPPI RGGWCCY 1 cut(s) 1687
PstNI CAGNNNCTG 1 cut(s) 3612
PsuI RGATCY 5 cut(s) 1057, 1593, 3031, 3289, 3527
PvuII CAGCTG 2 cut(s) 1516, 2562
RseI CAYNNNNRTG 2 cut(s) 1413, 2282
SacI GAGCTC 1 cut(s) 3852
SapI GCTCTTC 1 cut(s) 3258
SatI GCNGC 9 cut(s) 134, 1893, 1983, 2107, 2483, 2961, 3247, 3948, 3951
Sau96I GGNCC 3 cut(s) 439, 1687, 3620
ScaI AGTACT 2 cut(s) 1263, 2580
SchI GAGTC 5 cut(s) 599, 629, 1820, 2814, 3079
ScrFI CCNGG 1 cut(s) 3546
SduI GDGCHC 8 cut(s) 1254, 1420, 1903, 2218, 3225, 3852, 3896, 3929
SfcI CTRYAG 1 cut(s) 3741
SinI GGWCC 3 cut(s) 439, 1687, 3620
SmiMI CAYNNNNRTG 2 cut(s) 1413, 2282
SnaBI TACGTA 1 cut(s) 2350
SseBI AGGCCT 1 cut(s) 3348
SsiI CCGC 3 cut(s) 431, 1356, 2897
SspI AATATT 2 cut(s) 2183, 3489
SspMI CTAG 6 cut(s) 5, 1653, 2205, 3155, 3479, 3812
SstI GAGCTC 1 cut(s) 3852
StuI AGGCCT 1 cut(s) 3348
StyD4I CCNGG 1 cut(s) 3544
StyI CCWWGG 4 cut(s) 1274, 1314, 1398, 1683
TaaI ACNGT 3 cut(s) 640, 3585, 3880
TaiI ACGT 3 cut(s) 1920, 2352, 2766
TatI WGTACW 5 cut(s) 1261, 2578, 2832, 3159, 3674
TscAI CASTG 5 cut(s) 748, 764, 1016, 1157, 2773
TseFI GTSAC 5 cut(s) 92, 2291, 2504, 2764, 3133
TseI GCWGC 9 cut(s) 133, 1892, 1982, 2106, 2482, 2960, 3246, 3947, 3950
Tsp45I GTSAC 5 cut(s) 92, 2291, 2504, 2764, 3133
TspGWI ACGGA 2 cut(s) 2647, 3152
TspRI CASTG 5 cut(s) 748, 764, 1016, 1157, 2773
Vha464I CTTAAG 3 cut(s) 677, 881, 3191
VneI GTGCAC 1 cut(s) 1416
VpaK11BI GGWCC 3 cut(s) 439, 1687, 3620
VspI ATTAAT 2 cut(s) 360, 3102
XbaI TCTAGA 2 cut(s) 3478, 3811
XceI RCATGY 4 cut(s) 1412, 2113, 3635, 3673
XcmI CCANNNNNNNNNTGG 2 cut(s) 756, 3852
XmnI GAANNNNTTC 3 cut(s) 18, 1263, 1784
XspI CTAG 6 cut(s) 5, 1653, 2205, 3155, 3479, 3812
ZraI GACGTC 1 cut(s) 2764
ZrmI AGTACT 2 cut(s) 1263, 2580
Zsp2I ATGCAT 4 cut(s) 2002, 2279, 2289, 3023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.