Rw5G039660

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69961194 .. 69973853
12660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039660.1

Sequence Viewer

Length: 3492 bp
ATGGCCTCGAGCACCCAAACAGCATCCTCATCTCATCCTTCGTCATCTGCTCCTCGGAGGAAGTACGATGTTTTTCTGAGTTTCAGAGGTGAAGATACTCGCAGGACTATTGTCCCCTTTATATACCGGGAATTGCAAAACAGAGAAATTGAAACATTCATGGATGACACAGGGCTTAGCAGAGGGACATTTATTGCTCCGAGTCTGCTAACAGCAATCCAAGAATCCAGGTTAGCGATCGTTGTTCTCTCACCAAACTATGCGTATTCTAGATGGTGTTTAGACGAATTGACAATGATCATTCAGTGCATGGAAGATAAGAGCACAACAGTTATCCCAGTCTTTTATGATGTGGTTCCCTCTGATGTGCGACATCAGAAAGGGAGTTTTGCAAGAGCCTTCACTAAGCACCAAGAAAGGCTTGCGCAGGACGATGACAACCCATTCAAGTTGAACCAGTGGGGAGTTGCTTTAACAAAAGTTGCTGGTCTCTCTGGCTTGGAATCAAACAAATATAGTTCTGACTTAGAACTTGTGAAAGGCATTGTGGAAACCGTGTGCAGCAAATTACGACCTATTGATGTTGAGTGGGCATTGTCATCTACCGGGGATTTTGTAGCATTTGAAGCAACAACACGAGCCATGGACAAGGTAATGAGGGCACTTCTAGATGATGAGATCACTGCCATTGGGGTCTTTGGTATGGGAGGCGTTGGCAAGACAAGTATGGTGAAACATGTCGGTGCACAAGCCCGGAAAATTGGGCTCTTTAATTATGTGATTATGGCTGTCGTATCCCAAACACCTAATTTGAGAAATATTCAAGGCACACTGGCCGATTTGCTGGGACTAAAATTGTTGGAGGAGACAGAATTTGGAAGAGCGGTTAGATTGATGGAGAAAATAATGAAAGGAAATCGGATCCTTATAATCTTGGACGACATTTGGGAGAGAATAGATTTGTCTACTATTGGAATACCGTGCCATGATGAGCTTCAAAAGTGCAGTTCCAAAATCTTATTCACTACAAGGAGATTGAATGTTTGCCATTCGATGGAGAGCCAAGCAAGCATTCCTCTTCCAGTCTTATCAGAAGATGATGCTTGGAACCTGTTTGTGAAAAAAGCAAGAAAGTCTTTTCATGAATCTACCAGCTTCTATGATGTAGCGAGGACGGTAGCTAAAGAATGTGCTGGTCTCCCAGTTGCATTGGTAGCGGTTGCAAGGGCACTTGGAGATAAAGACTTAGATGATTGGACGGAAGCAGCTCGACGACTAAAGGCTGCTCAACCTGTCAACTATGAAGATGAGAGAGATGTGTTCAGATGTATAAATTTGAGCTATGATTACCTGAGACCTGATGATAACAACATTGCCAAGTCATTCTTCTTGCTTTGCTGTCTATTCCCTGAAGATTTTAATATCCAGGTGGAAAACTTGTTGGTGTATGCAATTGGGAAAGGAATATTTCAAGATGCCCACACAATACACGAAGCCAGAGCCAAAGCCCATTCGGTGGTCAAGTACCTGAAAGCTTCTAGCTTGCTTTTGGACAGTGCATACCCCGGATTCGTGAAGATGCATGATGTCATTCGAGATGTGGCCATATCAATTTCATCATCCAAAGATGGCCCACGGTTTTTGGTTAAAGCTGGTTGTGAATTAAGGGTTTGGCCAACGATTGACATTGCTCATGAAGGCTACTCTGCAATCTCGCTAATGAGGAATGAAATTCACGAGCTTCCCAAAAAGTTGGTATGTTCAAAACTCCAGATTTTATTACTACAGAGCAATATTATAAATGAGATTCCAAAACCTTTCTTTCAAAGTCCCAATGAATTGAGGGTCTTAGATCTCAGCGAGACTAGTATTTCGTCTCTGCCCTCATCATCAAGTCTTCTAACCAACCTCCAGGCTTTGTATTTAGATGAATGCAAGAGAATAGTTGAATTATCCATAATCGGAAAACTGAAGAACCTTCAGATTCTTAGTATGCAGAACCTTGATATTATTAACATGGGAACTCGTGAAAAAGCATTGCCGAAAGAAATAGGACGCTTAACCAAGCTTAAGATGTTGGATGTTACGGATGCATGTTTTGAAACAGTTCCATCTAAAGTGATATCAAACTTGAATGGATTAGAAGAATTGTACATGAAATGTGACTTTTCTGACTGGGGCAGTAGAATAGCTGAGGGCAGCAGTTTATTTTCTTCTGTTTTTGGCACTGCCAAGAATGCCAGCTTTGATGAGTTAACAAGATTATCACGCTTAAACAGTTTGAGAGTTCACATATCTGATGTAGAATGCTTGCCCCGAAAAGTTAGATTCAATCCCAATTGGGTCAACTTTGATATATGTATCAGCAGAAAAGATTTTATGAAGCAGTCCTATTACAGACGCTTTTTCGAGAGGAAACTAACCGTCAAGTTGGATCCATGTCAACATCTCTTTCGGTATGTGACTCTAAATACAAGTACGGGGAGTTTACCAGAATGGTTTATCAAGGTGGTGACAGTGAAGGCAGAGATACTAGAGTACATAGGGTACAGAGGCTTAGGGAACCTTCTTGTGGAATATCACCATGGGAGATTACAGGGGCTGAAGTACCTTGGTGTAATTGAGTGCTTTGCTGTGGATGTAGTGTTGATGAGCTCGATAACAACAAGGGTTCCAAATAAACCTGTGCTTGAGAGCTTGATAGAATTGTATCTGGAAGAGGTCCATGGCCTGCGGGGGTTATGTTATGGTGAGTTACCACGTGGGTCTCTGTGCAATCTAAAGTTGTTAAATGTGAGAAATTGCCTGGAATTGATGGGTGCACTTTTACAGCCAAATTTGTTGCGAAGGCTACACAATCTGGAAATACTACATTGTGAGTACACGGAGGATGCGAAAGAAACCGAATTGGAATATGTGTTTGGATTTGGATGGCTAGAAACAGAACAAACTGCCTTACCAAAATTGAGAGAGATAACATTATGTAATATTGAGAACCTCTTAAGCATATGCATTGATCATCCTCCCAAGGGGGTCTTCCGTAATCTTAACAGTCTGGCAATTCGTGGCTGCCACAAACTGAAATATCTGTTCGAGGACAATGTAGCTCAGTCTCTTTTCCAATTGGAACTCCTTTGGGTGGACGGTTGCTCTGACTTGGTAACAGTTATTGTTCCAAGATGGGAAACAGAGATCAACAACCTAGTTTTTCCAAAATTGAAGGAAATATATTTAAATAGTCTTCCACATCTCACAGGGATCTACACACAAAAGAGGGTTGTGTGCCCTTCATTGGAACACTTGTACGTGTACAGTTGCCCCGGACTTTCAAACCTTGCCGCTTCTGACTTCCAAAGCAAGAAACACGTCCAAGCCAATAATGAAGGACATTTTGCGATTATATTTGAAAGATTGTTCCACCGCCAATTCAATCCAGAAGAGTTTCCTCAGTTCTTCTCCAAAAAAGAAAGAGATTTCTCAATTGGCTCTTAA

Protein Analysis

1163

Amino Acids

131.82

Weight (kDa)

6.64

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 193 1.4e-48 TIR domain
TIR_2 PF13676 24 - 120 8.6e-15 TIR domain
NB-ARC PF00931 213 - 377 2.3e-30 NB-ARC domain
LRR_14 PF23598 589 - 668 3.6e-06 Leucine-rich repeat region
LRR_8 PF13855 589 - 645 1.9e-08 Leucine rich repeat
LRR_14 PF23598 675 - 768 5.3e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 901 - 1050 6.6e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 929, 1799
Acc16I TGCGCA 1 cut(s) 426
AccB7I CCANNNNNTGG 2 cut(s) 1054, 1516
AccBSI CCGCTC 1 cut(s) 884
AccI GTMKAC 1 cut(s) 965
AciI CCGC 5 cut(s) 884, 1217, 2734, 3339, 3421
AclWI GGATC 5 cut(s) 916, 929, 2429, 2442, 3266
AcoI YGGCCR 3 cut(s) 834, 1602, 1673
AcsI RAATTY 4 cut(s) 872, 1333, 1731, 2836
AcuI CTGAAG 4 cut(s) 1431, 1964, 1991, 2624
AcvI CACGTG 1 cut(s) 2762
AfiI CCNNNNNNNGG 6 cut(s) 1054, 1516, 2572, 3029, 3139, 3292
AflII CTTAAG 2 cut(s) 2069, 3001
AflIII ACRYGT 3 cut(s) 736, 3306, 3364
AhlI ACTAGT 1 cut(s) 1865
AjiI CACGTC 1 cut(s) 3367
AjnI CCWGG 4 cut(s) 227, 1425, 1911, 2805
Alw21I GWGCWC 5 cut(s) 14, 326, 748, 2657, 2824
Alw26I GTCTC 8 cut(s) 494, 860, 1202, 1348, 1856, 1881, 2772, 3117
Alw44I GTGCAC 2 cut(s) 744, 2820
AlwI GGATC 5 cut(s) 916, 929, 2429, 2442, 3266
AlwNI CAGNNNCTG 1 cut(s) 2602
Ama87I CYCGRG 1 cut(s) 7
AoxI GGCC 6 cut(s) 3, 834, 1602, 1630, 1673, 2728
ApaLI GTGCAC 2 cut(s) 744, 2820
ApeKI GCWGC 5 cut(s) 561, 1265, 1283, 2199, 3069
ApoI RAATTY 4 cut(s) 872, 1333, 1731, 2836
ArsI GACNNNNNNTTYG 2 cut(s) 1855, 1887
Asp700I GAANNNNTTC 2 cut(s) 2106, 3441
AspLEI GCGC 1 cut(s) 427
AspS9I GGNCC 2 cut(s) 1631, 2722
AsuC2I CCSGG 5 cut(s) 128, 607, 754, 1566, 3321
AsuHPI GGTGA 6 cut(s) 101, 243, 742, 2524, 2573, 2762
AvaI CYCGRG 1 cut(s) 7
AvaII GGWCC 1 cut(s) 2722
BaeGI GKGCMC 5 cut(s) 664, 748, 1231, 2824, 3287
BaeI ACNNNNGTAYC 2 cut(s) 2522, 2555
BalI TGGCCA 2 cut(s) 1604, 1675
BamHI GGATCC 2 cut(s) 921, 2434
BanII GRGCYC 2 cut(s) 768, 2657
BauI CACGAG 3 cut(s) 636, 1736, 2025
BbrPI CACGTG 1 cut(s) 2762
BbsI GAAGAC 3 cut(s) 1889, 3028, 3233
Bbv12I GWGCWC 5 cut(s) 14, 326, 748, 2657, 2824
BbvCI CCTCAGC 1 cut(s) 2193
BbvI GCAGC 5 cut(s) 573, 1270, 1277, 2211, 3056
BccI CCATC 8 cut(s) 267, 889, 1048, 1622, 2119, 2809, 2925, 3174
BciT130I CCWGG 4 cut(s) 229, 1427, 1913, 2807
BciVI GTATCC 1 cut(s) 805
BclI TGATCA 2 cut(s) 297, 3016
BcnI CCSGG 5 cut(s) 128, 607, 754, 1566, 3321
BcoDI GTCTC 8 cut(s) 494, 860, 1202, 1348, 1856, 1881, 2772, 3117
BcuI ACTAGT 1 cut(s) 1865
BfaI CTAG 7 cut(s) 270, 668, 1539, 1866, 2534, 2936, 3203
BfmI CTRYAG 1 cut(s) 1784
BfrI CTTAAG 2 cut(s) 2069, 3001
BfuI GTATCC 1 cut(s) 805
BglII AGATCT 1 cut(s) 1852
BisI GCNGC 6 cut(s) 562, 1266, 1284, 2200, 3070, 3339
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 6 cut(s) 563, 1267, 1285, 2201, 3071, 3340
Bme1390I CCNGG 9 cut(s) 128, 229, 607, 754, 1427, 1566, 1913, 2807, 3321
Bme18I GGWCC 1 cut(s) 2722
BmeT110I CYCGRG 1 cut(s) 7
BmgBI CACGTC 1 cut(s) 3367
BmgT120I GGNCC 2 cut(s) 1631, 2722
BmiI GGNNCC 6 cut(s) 357, 923, 1109, 2436, 2564, 2673
BmrFI CCNGG 9 cut(s) 128, 229, 607, 754, 1427, 1566, 1913, 2807, 3321
BmrI ACTGGG 3 cut(s) 332, 1196, 2185
BmsI GCATC 6 cut(s) 32, 1090, 1465, 1569, 2080, 2881
BmuI ACTGGG 3 cut(s) 332, 1196, 2185
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 3 cut(s) 1889, 3028, 3233
BpmI CTGGAG 2 cut(s) 1754, 1895
Bpu10I CCTNAGC 2 cut(s) 2193, 2557
Bpu1102I GCTNAGC 1 cut(s) 176
BpuEI CTTGAG 1 cut(s) 2711
BpuMI CCSGG 5 cut(s) 128, 607, 754, 1566, 3321
BsaAI YACGTR 2 cut(s) 2762, 3307
BsaI GGTCTC 4 cut(s) 494, 1202, 1348, 2772
Bsc4I CCNNNNNNNGG 6 cut(s) 1054, 1516, 2572, 3029, 3139, 3292
Bse1I ACTGG 6 cut(s) 338, 457, 837, 1082, 1202, 2180
Bse3DI GCAATG 3 cut(s) 1371, 1686, 2036
BseBI CCWGG 4 cut(s) 229, 1427, 1913, 2807
BseLI CCNNNNNNNGG 6 cut(s) 1054, 1516, 2572, 3029, 3139, 3292
BseMI GCAATG 3 cut(s) 1371, 1686, 2036
BseMII CTCAG 6 cut(s) 68, 1343, 1870, 2184, 3122, 3461
BseNI ACTGG 6 cut(s) 338, 457, 837, 1082, 1202, 2180
BseRI GAGGAG 2 cut(s) 42, 878
BseSI GKGCMC 5 cut(s) 664, 748, 1231, 2824, 3287
BseXI GCAGC 5 cut(s) 573, 1270, 1277, 2211, 3056
BseYI CCCAGC 1 cut(s) 844
BsgI GTGCAG 2 cut(s) 580, 1024
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 6 cut(s) 5, 836, 1604, 1632, 1675, 2730
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 5 cut(s) 14, 326, 748, 2657, 2824
BsiHKCI CYCGRG 1 cut(s) 7
BsiSI CCGG 5 cut(s) 127, 606, 754, 1566, 3321
BslFI GGGAC 4 cut(s) 98, 199, 861, 1815
BslI CCNNNNNNNGG 6 cut(s) 1054, 1516, 2572, 3029, 3139, 3292
BsmAI GTCTC 8 cut(s) 494, 860, 1202, 1348, 1856, 1881, 2772, 3117
BsmBI CGTCTC 1 cut(s) 1881
BsmFI GGGAC 4 cut(s) 98, 199, 861, 1815
BsmI GAATGC 4 cut(s) 1071, 1937, 2242, 2312
BsnI GGCC 6 cut(s) 5, 836, 1604, 1632, 1675, 2730
Bso31I GGTCTC 4 cut(s) 494, 1202, 1348, 2772
BsoBI CYCGRG 1 cut(s) 7
Bsp1286I GDGCHC 9 cut(s) 14, 326, 664, 748, 768, 1231, 2657, 2824, 3287
Bsp1407I TGTACA 2 cut(s) 2151, 3309
Bsp143I GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
Bsp1720I GCTNAGC 1 cut(s) 176
Bsp19I CCATGG 3 cut(s) 642, 2584, 2725
BspACI CCGC 5 cut(s) 884, 1217, 2734, 3339, 3421
BspANI GGCC 6 cut(s) 5, 836, 1604, 1632, 1675, 2730
BspCNI CTCAG 6 cut(s) 69, 1344, 1869, 2185, 3121, 3460
BspHI TCATGA 2 cut(s) 1141, 1693
BspLI GGNNCC 6 cut(s) 357, 923, 1109, 2436, 2564, 2673
BspPI GGATC 5 cut(s) 916, 929, 2429, 2442, 3266
BspQI GCTCTTC 1 cut(s) 874
BspTI CTTAAG 2 cut(s) 2069, 3001
BspTNI GGTCTC 4 cut(s) 494, 1202, 1348, 2772
BsrBI CCGCTC 1 cut(s) 884
BsrDI GCAATG 3 cut(s) 1371, 1686, 2036
BsrGI TGTACA 2 cut(s) 2151, 3309
BsrI ACTGG 6 cut(s) 338, 457, 837, 1082, 1202, 2180
BssMI GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
BssSI CACGAG 3 cut(s) 636, 1736, 2025
BssT1I CCWWGG 5 cut(s) 642, 2584, 2611, 2725, 3027
Bst2BI CACGAG 3 cut(s) 636, 1736, 2025
Bst2UI CCWGG 4 cut(s) 229, 1427, 1913, 2807
Bst6I CTCTTC 4 cut(s) 874, 1083, 2712, 3432
BstAFI CTTAAG 2 cut(s) 2069, 3001
BstAUI TGTACA 2 cut(s) 2151, 3309
BstBAI YACGTR 2 cut(s) 2762, 3307
BstC8I GCNNGC 6 cut(s) 423, 1069, 1544, 2242, 2312, 2732
BstDSI CCRYGG 4 cut(s) 642, 1634, 2584, 2725
BstHHI GCGC 1 cut(s) 427
BstKTI GATC 9 cut(s) 240, 300, 681, 924, 1855, 2437, 3019, 3195, 3261
BstMAI GTCTC 8 cut(s) 494, 860, 1202, 1348, 1856, 1881, 2772, 3117
BstMBI GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 4 cut(s) 626, 1068, 1214, 2237
BstNI CCWGG 4 cut(s) 229, 1427, 1913, 2807
BstNSI RCATGY 2 cut(s) 740, 2097
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 9 cut(s) 126, 227, 605, 752, 1425, 1564, 1911, 2805, 3319
BstSFI CTRYAG 1 cut(s) 1784
BstSLI GKGCMC 5 cut(s) 664, 748, 1231, 2824, 3287
BstV1I GCAGC 5 cut(s) 573, 1270, 1277, 2211, 3056
BstV2I GAAGAC 3 cut(s) 1889, 3028, 3233
BstX2I RGATCY 4 cut(s) 921, 1852, 2434, 3258
BstXI CCANNNNNNTGG 1 cut(s) 1753
BstYI RGATCY 4 cut(s) 921, 1852, 2434, 3258
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 6 cut(s) 5, 836, 1604, 1632, 1675, 2730
BtgI CCRYGG 4 cut(s) 642, 1634, 2584, 2725
BtrI CACGTC 1 cut(s) 3367
BtsI GCAGTG 2 cut(s) 681, 2226
BtsIMutI CAGTG 7 cut(s) 311, 464, 681, 830, 1561, 2226, 2523
Cac8I GCNNGC 6 cut(s) 423, 1069, 1544, 2242, 2312, 2732
CaiI CAGNNNCTG 1 cut(s) 2602
CciI TCATGA 2 cut(s) 1141, 1693
CfoI GCGC 1 cut(s) 427
Cfr13I GGNCC 2 cut(s) 1631, 2722
CseI GACGC 2 cut(s) 2064, 2409
CspCI CAANNNNNGTGG 2 cut(s) 1623, 1658
DpnI GATC 9 cut(s) 239, 299, 680, 923, 1854, 2436, 3018, 3194, 3260
DpnII GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
DraI TTTAAA 1 cut(s) 3234
EaeI YGGCCR 3 cut(s) 834, 1602, 1673
Eam1104I CTCTTC 4 cut(s) 874, 1083, 2712, 3432
EarI CTCTTC 4 cut(s) 874, 1083, 2712, 3432
Ecl136II GAGCTC 1 cut(s) 2655
Eco130I CCWWGG 5 cut(s) 642, 2584, 2611, 2725, 3027
Eco24I GRGCYC 2 cut(s) 768, 2657
Eco31I GGTCTC 4 cut(s) 494, 1202, 1348, 2772
Eco32I GATATC 1 cut(s) 2124
Eco47I GGWCC 1 cut(s) 2722
Eco53kI GAGCTC 1 cut(s) 2655
Eco57I CTGAAG 4 cut(s) 1431, 1964, 1991, 2624
Eco72I CACGTG 1 cut(s) 2762
Eco88I CYCGRG 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 2655
EcoRII CCWGG 4 cut(s) 227, 1425, 1911, 2805
EcoRV GATATC 1 cut(s) 2124
EcoT14I CCWWGG 5 cut(s) 642, 2584, 2611, 2725, 3027
EcoT22I ATGCAT 3 cut(s) 1584, 2095, 3014
EcoT38I GRGCYC 2 cut(s) 768, 2657
ErhI CCWWGG 5 cut(s) 642, 2584, 2611, 2725, 3027
Esp3I CGTCTC 1 cut(s) 1881
FalI AAGNNNNNCTT 8 cut(s) 405, 437, 1120, 1152, 1370, 1402, 3020, 3052
FaqI GGGAC 4 cut(s) 98, 199, 861, 1815
FauI CCCGC 1 cut(s) 2727
FauNDI CATATG 1 cut(s) 3008
FbaI TGATCA 2 cut(s) 297, 3016
FblI GTMKAC 1 cut(s) 965
Fnu4HI GCNGC 6 cut(s) 562, 1266, 1284, 2200, 3070, 3339
FriOI GRGCYC 2 cut(s) 768, 2657
Fsp4HI GCNGC 6 cut(s) 562, 1266, 1284, 2200, 3070, 3339
FspBI CTAG 7 cut(s) 270, 668, 1539, 1866, 2534, 2936, 3203
FspI TGCGCA 1 cut(s) 426
GlaI GCGC 1 cut(s) 426
GluI GCNGC 6 cut(s) 562, 1266, 1284, 2200, 3070, 3339
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 2 cut(s) 1754, 1895
HaeIII GGCC 6 cut(s) 5, 836, 1604, 1632, 1675, 2730
HapII CCGG 5 cut(s) 127, 606, 754, 1566, 3321
HgaI GACGC 2 cut(s) 2064, 2409
HhaI GCGC 1 cut(s) 427
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HincII GTYRAC 4 cut(s) 1297, 2256, 2347, 2444
HindII GTYRAC 4 cut(s) 1297, 2256, 2347, 2444
HindIII AAGCTT 2 cut(s) 1533, 2066
HinfI GANTC 9 cut(s) 202, 224, 503, 1145, 1569, 1807, 1984, 2328, 2464
HpaI GTTAAC 1 cut(s) 2256
HpaII CCGG 5 cut(s) 127, 606, 754, 1566, 3321
HphI GGTGA 6 cut(s) 101, 243, 742, 2524, 2573, 2762
Hpy99I CGWCG 1 cut(s) 1275
HpyCH4IV ACGT 3 cut(s) 2761, 3306, 3366
HpyF10VI GCNNNNNNNGC 4 cut(s) 626, 1068, 1214, 2237
HpySE526I ACGT 3 cut(s) 2761, 3306, 3366
HspAI GCGC 1 cut(s) 425
Ksp22I TGATCA 2 cut(s) 297, 3016
KspAI GTTAAC 1 cut(s) 2256
Kzo9I GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
LguI GCTCTTC 1 cut(s) 874
LmnI GCTCC 2 cut(s) 55, 202
Lsp1109I GCAGC 5 cut(s) 573, 1270, 1277, 2211, 3056
LweI GCATC 6 cut(s) 32, 1090, 1465, 1569, 2080, 2881
MaeI CTAG 7 cut(s) 270, 668, 1539, 1866, 2534, 2936, 3203
MaeII ACGT 3 cut(s) 2761, 3306, 3366
MaeIII GTNAC 6 cut(s) 2083, 2162, 2461, 2512, 2754, 3160
MalI GATC 9 cut(s) 239, 299, 680, 923, 1854, 2436, 3018, 3194, 3260
MbiI CCGCTC 1 cut(s) 884
MboI GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
MfeI CAATTG 4 cut(s) 1452, 2338, 3122, 3480
MflI RGATCY 4 cut(s) 921, 1852, 2434, 3258
MhlI GDGCHC 9 cut(s) 14, 326, 664, 748, 768, 1231, 2657, 2824, 3287
MlsI TGGCCA 2 cut(s) 1604, 1675
MluNI TGGCCA 2 cut(s) 1604, 1675
MlyI GAGTC 2 cut(s) 211, 2458
MmeI TCCRAC 3 cut(s) 840, 2058, 2412
Mox20I TGGCCA 2 cut(s) 1604, 1675
Mph1103I ATGCAT 3 cut(s) 1584, 2095, 3014
MroXI GAANNNNTTC 2 cut(s) 2106, 3441
MscI TGGCCA 2 cut(s) 1604, 1675
MslI CAYNNNNRTG 1 cut(s) 741
Msp20I TGGCCA 2 cut(s) 1604, 1675
MspCI CTTAAG 2 cut(s) 2069, 3001
MspI CCGG 5 cut(s) 127, 606, 754, 1566, 3321
MspR9I CCNGG 9 cut(s) 128, 229, 607, 754, 1427, 1566, 1913, 2807, 3321
MunI CAATTG 4 cut(s) 1452, 2338, 3122, 3480
Mva1269I GAATGC 4 cut(s) 1071, 1937, 2242, 2312
MvaI CCWGG 4 cut(s) 229, 1427, 1913, 2807
MwoI GCNNNNNNNGC 4 cut(s) 626, 1068, 1214, 2237
NciI CCSGG 5 cut(s) 128, 607, 754, 1566, 3321
NcoI CCATGG 3 cut(s) 642, 2584, 2725
NdeI CATATG 1 cut(s) 3008
NdeII GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
NlaIV GGNNCC 6 cut(s) 357, 923, 1109, 2436, 2564, 2673
NmuCI GTSAC 3 cut(s) 2162, 2461, 2512
NsbI TGCGCA 1 cut(s) 426
NsiI ATGCAT 3 cut(s) 1584, 2095, 3014
NspI RCATGY 2 cut(s) 740, 2097
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 2 cut(s) 1141, 1693
PciI ACATGT 1 cut(s) 736
PciSI GCTCTTC 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 2891
PctI GAATGC 4 cut(s) 1071, 1937, 2242, 2312
PdmI GAANNNNTTC 2 cut(s) 2106, 3441
PfeI GAWTC 7 cut(s) 224, 503, 1145, 1569, 1807, 1984, 2328
PflMI CCANNNNNTGG 2 cut(s) 1054, 1516
PkrI GCNGC 6 cut(s) 563, 1267, 1285, 2201, 3071, 3340
Ple19I CGATCG 1 cut(s) 240
PleI GAGTC 2 cut(s) 210, 2458
PmaCI CACGTG 1 cut(s) 2762
PmlI CACGTG 1 cut(s) 2762
PpsI GAGTC 2 cut(s) 210, 2458
Ppu21I YACGTR 2 cut(s) 2762, 3307
PscI ACATGT 1 cut(s) 736
PshAI GACNNNNGTC 1 cut(s) 110
PsiI TTATAA 2 cut(s) 929, 1799
Psp124BI GAGCTC 1 cut(s) 2657
Psp6I CCWGG 4 cut(s) 227, 1425, 1911, 2805
PspCI CACGTG 1 cut(s) 2762
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 4 cut(s) 227, 1425, 1911, 2805
PspN4I GGNNCC 6 cut(s) 357, 923, 1109, 2436, 2564, 2673
PspPI GGNCC 2 cut(s) 1631, 2722
PspXI VCTCGAGB 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 2602
PsuI RGATCY 4 cut(s) 921, 1852, 2434, 3258
PvuI CGATCG 1 cut(s) 240
RseI CAYNNNNRTG 1 cut(s) 741
SacI GAGCTC 1 cut(s) 2657
SapI GCTCTTC 1 cut(s) 874
SatI GCNGC 6 cut(s) 562, 1266, 1284, 2200, 3070, 3339
Sau3AI GATC 9 cut(s) 237, 297, 678, 921, 1852, 2434, 3016, 3192, 3258
Sau96I GGNCC 2 cut(s) 1631, 2722
SchI GAGTC 2 cut(s) 211, 2458
ScrFI CCNGG 9 cut(s) 128, 229, 607, 754, 1427, 1566, 1913, 2807, 3321
SduI GDGCHC 9 cut(s) 14, 326, 664, 748, 768, 1231, 2657, 2824, 3287
SfaNI GCATC 6 cut(s) 32, 1090, 1465, 1569, 2080, 2881
SfcI CTRYAG 1 cut(s) 1784
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 1 cut(s) 2722
SlaI CTCGAG 1 cut(s) 7
SmiI ATTTAAAT 1 cut(s) 3234
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 4 cut(s) 7, 2069, 2690, 3001
SmoI CTYRAG 4 cut(s) 7, 2069, 2690, 3001
SpeI ACTAGT 1 cut(s) 1865
SsiI CCGC 5 cut(s) 884, 1217, 2734, 3339, 3421
SspI AATATT 4 cut(s) 820, 1467, 1795, 2989
SspMI CTAG 7 cut(s) 270, 668, 1539, 1866, 2534, 2936, 3203
SstI GAGCTC 1 cut(s) 2657
StyD4I CCNGG 9 cut(s) 126, 227, 605, 752, 1425, 1564, 1911, 2805, 3319
StyI CCWWGG 5 cut(s) 642, 2584, 2611, 2725, 3027
SwaI ATTTAAAT 1 cut(s) 3234
TaiI ACGT 3 cut(s) 2764, 3309, 3369
TaqI TCGA 7 cut(s) 8, 1052, 1270, 1594, 2409, 2657, 3093
TatI WGTACW 4 cut(s) 2151, 2538, 2880, 3309
TauI GCSGC 1 cut(s) 3341
TfiI GAWTC 7 cut(s) 224, 503, 1145, 1569, 1807, 1984, 2328
TscAI CASTG 7 cut(s) 311, 464, 688, 837, 1561, 2233, 2523
TseFI GTSAC 3 cut(s) 2162, 2461, 2512
TseI GCWGC 5 cut(s) 561, 1265, 1283, 2199, 3069
Tsp45I GTSAC 3 cut(s) 2162, 2461, 2512
TspGWI ACGGA 4 cut(s) 1274, 2102, 2900, 3029
TspRI CASTG 7 cut(s) 311, 464, 688, 837, 1561, 2233, 2523
Van91I CCANNNNNTGG 2 cut(s) 1054, 1516
Vha464I CTTAAG 2 cut(s) 2069, 3001
VneI GTGCAC 2 cut(s) 744, 2820
VpaK11BI GGWCC 1 cut(s) 2722
XapI RAATTY 4 cut(s) 872, 1333, 1731, 2836
XbaI TCTAGA 2 cut(s) 269, 667
XceI RCATGY 2 cut(s) 740, 2097
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 965
XmnI GAANNNNTTC 2 cut(s) 2106, 3441
XspI CTAG 7 cut(s) 270, 668, 1539, 1866, 2534, 2936, 3203
Zsp2I ATGCAT 3 cut(s) 1584, 2095, 3014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.