FvH4_3g43650

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000085
Physical Location & Seq
Reverse (-)
36355878 .. 36360186
4309 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43650.t2

Sequence Viewer

Length: 3222 bp
ATGGCTTCCAGCTCCCAAGTAGCCTCTGCATCTCCTTCAGCAGATCCATGTTGGAAGTATGATGTCTTTTTGAGTTTCAGGGGTGCAGACACTCGCAAAGGGATCACAGCCGACATATACGATGGACTCGATCGAAGAGGCATTAAAACATTCATGGATGATCCAGATCTTAAAGTCGGTGATGTCATTTCTCCCACTCTACAAGAGGCAATTAAGAAATCAAGGTTTGCAATTGTTGTTCTCTCGAAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTGAGTGCATGAAAGACCAGAACAGGATTTTGCCACTTTTTTACCATGTTGATCCTTGTGATGTTCGATATCAGAAGAGGAGTTTTGAAGAAGCTTTCTCTAACCATGAAACCTCAGGCAGACATGAATCAGAGAAGGTGAAGCAATGGAGAGATGCTTTAAACAAAGTGGCCAATTTCTCCGGGTGGAGCACCCAACAATATAAGACTCACAAAGAACTTGTTAACGTCATTGTGGAATTTGTCAGCACGAAAGTAGTACCTGACTCGATTGAGTCCACAGGAGATTTCGAAGTGTTTGAAGTAACAAGACGAGCCATTTATGAGGTTATGGAGGCCTTTAAAGATGATGAGATCACTGCCATTGGTGTCTACGGCATGGGAGGCGTTGGCAAGACAACCTTAGTGGAACATATCGGTACACAAGCCCGGAAAAGTGGGATTCTTGATTATGTGACAAAATGTGTAATATCCCAAAACCCTGACTTCGAAAGAATTCAAGATACATTGGCAGAGCAGCTGGGCTTTGAATTACATGAGAAGACAGAAATTGGAAGAGCAGCTAGATTGGGAAAGGAGATAAAGAAAAGGGAAAAAATCCTTGTAATCTTGGACGACATTTGGGAGAGAACGGACATATCAAGAATTGGAATTCCAAGCTACAAGGAACTTCACAAGTGCAATTCCAAAGTCCTGCTCACCACAAGGATAAGGAATGTTTGTCATGCCATGGAGTGCCAAAGAAAGATCACCCTCAACGTTCTATCTGAACAAGATTCTTGGGCCTTGTTTGTCAGAAAAGCAAGAAGGTATTTTGAATCCCCCAGTTTTGAGGGTGTGGCAAAGAAGGTAGCTAGAGAATGTAGGGGCTTACCTATTGCATTGATAACAGTTGCAAGGGCACTCGGAGACAAAAACTTGGTGGAGTGGAGGAGAGCAGCTCAGCGACTAGAGAAGTCACAAACTGCCAACCCTGACGATAAGGGAGAAGCATCCAAATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAGTCATACTTCTTGCTGTGTTGCTTATTCCCAGAAGACTATGACATCCCTATAGAAGACTTGTTCAGGTATGGGATCGGGAAAGGATTGTTTCAAGATGCCGAAACACTCGGAGAAGCGAGAGACAGAGCTGATTCCCTGGCCATGCACCTTAGATATTGTAGCTTGCTGTTGGATGGCAGTGACGAGACATTTGTAAGGATGCATGATGTTGTCCGGGATGCAGCCCTTCAAATTTCGCAATCTGAACATGGGTTTTTGGTGAAAGCTGGTTGTGGTTTAAAAGATTGGCCACGTCAATTACATGAAGACTGCTCTGCAATCTCTCTCGTGGACAATCATATCAGCAAGCTACCTGAAAATTTGGTATGTCCAAGACTCCAGATTTTGCTACTAAAAGGTAATTCATGTATAGAAGAAATATCAGAAACATTTTTTCAGAGTCCAAATGAATTAAGGGTCTTAGATCTTAGCTGGACTAGCCTTTCATTACTTCCCCAATCATCCAGTCTCCTAACCAACCTCCAAGCTTTGTATTTAGGTAGTTGTGACATAACTGACACTTCCATACTAGGAAAACTTAAACAACTTGAGATTCTTAGTATAAGAAATTCACGTTTAAAGGAATTGTCGATAAAAGTAAGAGGCTTGACCAATCTAAGGATGTTTGATAACACTGGTGGAAATATTGGGAGAATTCCACCCAAAGTGATGTCAAAGTTGCGTAATTTAGAAGAATTATACATAGAAGGATTTAAGGGGTGGGGGAGTAAAGTTGAGGGAGCAGGAGAAGAAACCAATGCTGGCTTTGACGAGGTAACTAGCTTGTCAAAATTAAGAGTTTTGAAGGTTAGTATGTTAGATGTAGAATGTATCCCCAAGTATGTTGAGTCGATACCAGCTGGGGTTGAGTTTCATATACGTATAGGTAGGTACGAGCCATACGAGATGGAACTCTATGATCTACATCGTGGACATCGTCGTAACACAATATGCTTGAAGCTTGACTTGATCAGTCGCTTACCGAAGTGGTTTTGCAAGGAGATAATGAAGATAGCAGAGAGTCTATGTTGGATAAAGGCCCGAAGGTTAATTGACATATTTGTGGAACATGAGGATGGGAGATTACAGGGACTGAAGCATCTACAAATAGTGGGTTCAAATCGAGGCATAACGGAGGTAATTCCCACAATAACATTGGTACCAAAAAGATCTGTGTTTGAGAATTTGGAAGAGTTCCACTTGGTTCGGCTGGATTGCGCGGGGTTGTGCGCCATTGAATTTCTACCACCTGGGTCTCTATTCAATTTGAAGGTCTTGAAGGTAGAAACTTGTTATAATTGGGGGATTATACTTTTACCATCTCCATTGTTACAGAGACTACCAAATTTGGAAGAAATGCTTGTTTATGGCACGGACGGGAGTAAATATATGTTTGGGTATGAGGCATTGCTAGTGTTACGGCAATTAAAATTGAGAAAGATAGATTTGTTCAATCTAGCTACAGTAAGCATATGTGATGGACCTACTCCACCTGCGACGTTTCAGAATCTGCAGAGTTTTTCCATTACATACTGCAAGTTTCAGGGAAGTCTCTTCAGCTATGATGTTGCTCAGTGTCTTTCTCAGTTGAACTCCTTAGAATTGGAGAATTGCCTTTATTTGGAAAGGATAGTAGAAGCAAGCAACAAGAAGATCATCCTTCCAAAATTGAAGCAATTATGTTTGAGTGAACTTCCAGTCTTGTACTACGACACCGCTACTTTTGATATGGAGTGCCCTTCGTTGGAAGACTTATTTTTGCGGAACTGCCCCAAATTTTCAGCCTCTTCTTCTGACTTCCACAGCAAGAAACAAGTTCAACTCAGATGGTGGACAGAGGACTAA

Protein Analysis

1074

Amino Acids

122.28

Weight (kDa)

6.22

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 134 1.7e-35 TIR domain
TIR_2 PF13676 2 - 67 3e-09 TIR domain
NB-ARC PF00931 158 - 315 2.1e-29 NB-ARC domain
LRR_8 PF13855 519 - 579 5.3e-08 Leucine rich repeat
LRR_14 PF23598 544 - 837 3.8e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 826 - 946 3.3e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2673
AarI CACCTGC 1 cut(s) 2878
Acc36I ACCTGC 1 cut(s) 2878
Acc65I GGTACC 1 cut(s) 2536
AccB1I GGYRCC 1 cut(s) 2536
AccI GTMKAC 1 cut(s) 666
AccII CGCG 1 cut(s) 2597
AciI CCGC 3 cut(s) 2597, 3093, 3139
AclI AACGTT 1 cut(s) 1053
AclWI GGATC 5 cut(s) 38, 110, 155, 341, 1418
AcoI YGGCCR 3 cut(s) 465, 1476, 1625
AcuI CTGAAG 3 cut(s) 21, 2492, 2917
AfaI GTAC 5 cut(s) 555, 715, 2270, 2538, 3083
AfiI CCNNNNNNNGG 3 cut(s) 1995, 2998, 3121
AhdI GACNNNNNGTC 1 cut(s) 1336
AjiI CACGTC 1 cut(s) 1631
AjnI CCWGG 2 cut(s) 1473, 2626
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 7 cut(s) 1197, 1452, 1517, 1850, 2637, 2707, 2933
AlwI GGATC 5 cut(s) 38, 110, 155, 341, 1418
AlwNI CAGNNNCTG 2 cut(s) 2470, 2887
AoxI GGCC 6 cut(s) 465, 630, 1077, 1476, 1625, 2415
ApeKI GCWGC 4 cut(s) 811, 854, 1232, 1559
ArsI GACNNNNNNTTYG 2 cut(s) 1511, 1543
Asp700I GAANNNNTTC 1 cut(s) 789
Asp718I GGTACC 1 cut(s) 2536
AspLEI GCGC 2 cut(s) 2597, 2609
AspS9I GGNCC 3 cut(s) 1077, 2416, 2858
AsuC2I CCSGG 3 cut(s) 478, 724, 1553
AsuHPI GGTGA 5 cut(s) 191, 445, 985, 1036, 1609
AsuII TTCGAA 2 cut(s) 585, 783
AvaII GGWCC 1 cut(s) 2858
AxyI CCTNAGG 1 cut(s) 409
BaeGI GKGCMC 2 cut(s) 1198, 3116
BaeI ACNNNNGTAYC 2 cut(s) 2520, 2553
BalI TGGCCA 3 cut(s) 467, 1478, 1627
BanI GGYRCC 1 cut(s) 2536
BauI CACGAG 1 cut(s) 1664
BbsI GAAGAC 5 cut(s) 842, 1377, 1398, 1650, 3132
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 4 cut(s) 823, 866, 1244, 1571
BccI CCATC 7 cut(s) 116, 1505, 2278, 2447, 2704, 2849, 3198
BceAI ACGGC 2 cut(s) 685, 2813
BciT130I CCWGG 2 cut(s) 1475, 2628
BciVI GTATCC 1 cut(s) 2219
BclI TGATCA 1 cut(s) 2346
BcnI CCSGG 3 cut(s) 478, 724, 1553
BcoDI GTCTC 7 cut(s) 1197, 1452, 1517, 1850, 2637, 2707, 2933
BfaI CTAG 8 cut(s) 858, 1149, 1244, 1815, 1907, 2157, 2789, 2834
BfmI CTRYAG 3 cut(s) 1386, 2838, 2888
BfuAI ACCTGC 1 cut(s) 2878
BfuI GTATCC 1 cut(s) 2219
BglII AGATCT 3 cut(s) 166, 1801, 2546
BisI GCNGC 4 cut(s) 812, 855, 1233, 1560
BlpI GCTNAGC 1 cut(s) 1236
BlsI GCNGC 4 cut(s) 813, 856, 1234, 1561
Bme1390I CCNGG 5 cut(s) 478, 724, 1475, 1553, 2628
Bme18I GGWCC 1 cut(s) 2858
BmeRI GACNNNNNGTC 1 cut(s) 1336
BmgBI CACGTC 1 cut(s) 1631
BmgT120I GGNCC 3 cut(s) 1077, 2416, 2858
BmiI GGNNCC 1 cut(s) 2538
BmrFI CCNGG 5 cut(s) 478, 724, 1475, 1553, 2628
BmrI ACTGGG 1 cut(s) 1113
BmsI GCATC 7 cut(s) 38, 439, 1295, 1423, 1527, 1546, 2485
BmuI ACTGGG 1 cut(s) 1113
BpiI GAAGAC 5 cut(s) 842, 1377, 1398, 1650, 3132
BplI GAGNNNNNCTC 2 cut(s) 1219, 1251
BpmI CTGGAG 1 cut(s) 1700
Bpu1102I GCTNAGC 1 cut(s) 1236
Bpu14I TTCGAA 2 cut(s) 585, 783
BpuEI CTTGAG 1 cut(s) 1946
BpuMI CCSGG 3 cut(s) 478, 724, 1553
BsaAI YACGTR 1 cut(s) 2258
BsaBI GATNNNNATC 2 cut(s) 165, 2301
BsaI GGTCTC 1 cut(s) 2637
BsaJI CCNNGG 3 cut(s) 1023, 1473, 2627
Bsc4I CCNNNNNNNGG 3 cut(s) 1995, 2998, 3121
Bse1I ACTGG 4 cut(s) 1119, 1842, 2017, 3074
Bse21I CCTNAGG 1 cut(s) 409
Bse3DI GCAATG 2 cut(s) 446, 2783
Bse8I GATNNNNATC 2 cut(s) 165, 2301
BseBI CCWGG 2 cut(s) 1475, 2628
BseDI CCNNGG 3 cut(s) 1023, 1473, 2627
BseJI GATNNNNATC 2 cut(s) 165, 2301
BseLI CCNNNNNNNGG 3 cut(s) 1995, 2998, 3121
BseMI GCAATG 2 cut(s) 446, 2783
BseMII CTCAG 5 cut(s) 423, 1250, 2963, 2975, 3214
BseNI ACTGG 4 cut(s) 1119, 1842, 2017, 3074
BseRI GAGGAG 2 cut(s) 388, 1240
BseSI GKGCMC 2 cut(s) 1198, 3116
BseXI GCAGC 4 cut(s) 823, 866, 1244, 1571
BseYI CCCAGC 2 cut(s) 814, 2237
BsgI GTGCAG 1 cut(s) 105
Bsh1236I CGCG 1 cut(s) 2597
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 6 cut(s) 467, 632, 1079, 1478, 1627, 2417
BshNI GGYRCC 1 cut(s) 2536
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 488
BsiSI CCGG 3 cut(s) 477, 724, 1552
BslFI GGGAC 1 cut(s) 2481
BslI CCNNNNNNNGG 3 cut(s) 1995, 2998, 3121
BsmAI GTCTC 7 cut(s) 1197, 1452, 1517, 1850, 2637, 2707, 2933
BsmFI GGGAC 1 cut(s) 2481
BsnI GGCC 6 cut(s) 467, 632, 1079, 1478, 1627, 2417
Bso31I GGTCTC 1 cut(s) 2637
Bsp119I TTCGAA 2 cut(s) 585, 783
Bsp1286I GDGCHC 3 cut(s) 488, 1198, 3116
Bsp1720I GCTNAGC 1 cut(s) 1236
Bsp19I CCATGG 1 cut(s) 1023
BspACI CCGC 3 cut(s) 2597, 3093, 3139
BspANI GGCC 6 cut(s) 467, 632, 1079, 1478, 1627, 2417
BspCNI CTCAG 5 cut(s) 422, 1249, 2962, 2974, 3213
BspFNI CGCG 1 cut(s) 2597
BspLI GGNNCC 1 cut(s) 2538
BspMAI CTGCAG 1 cut(s) 2892
BspMI ACCTGC 1 cut(s) 2878
BspPI GGATC 5 cut(s) 38, 110, 155, 341, 1418
BspQI GCTCTTC 1 cut(s) 844
BspT104I TTCGAA 2 cut(s) 585, 783
BspT107I GGYRCC 1 cut(s) 2536
BspTNI GGTCTC 1 cut(s) 2637
BsrDI GCAATG 2 cut(s) 446, 2783
BsrI ACTGG 4 cut(s) 1119, 1842, 2017, 3074
BssECI CCNNGG 3 cut(s) 1023, 1473, 2627
BssSI CACGAG 1 cut(s) 1664
BssT1I CCWWGG 1 cut(s) 1023
Bst2BI CACGAG 1 cut(s) 1664
Bst2UI CCWGG 2 cut(s) 1475, 2628
Bst4CI ACNGT 2 cut(s) 1186, 2842
Bst6I CTCTTC 6 cut(s) 130, 365, 844, 2562, 2936, 3169
BstBAI YACGTR 1 cut(s) 2258
BstBI TTCGAA 2 cut(s) 585, 783
BstC8I GCNNGC 4 cut(s) 1502, 1685, 2140, 3019
BstDSI CCRYGG 1 cut(s) 1023
BstFNI CGCG 1 cut(s) 2597
BstHHI GCGC 2 cut(s) 2597, 2609
BstMAI GTCTC 7 cut(s) 1197, 1452, 1517, 1850, 2637, 2707, 2933
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 2 cut(s) 678, 1815
BstNI CCWGG 2 cut(s) 1475, 2628
BstSCI CCNGG 5 cut(s) 476, 722, 1473, 1551, 2626
BstSFI CTRYAG 3 cut(s) 1386, 2838, 2888
BstSLI GKGCMC 2 cut(s) 1198, 3116
BstSNI TACGTA 1 cut(s) 2258
BstUI CGCG 1 cut(s) 2597
BstV1I GCAGC 4 cut(s) 823, 866, 1244, 1571
BstV2I GAAGAC 5 cut(s) 842, 1377, 1398, 1650, 3132
BstX2I RGATCY 4 cut(s) 43, 166, 1801, 2546
BstYI RGATCY 4 cut(s) 43, 166, 1801, 2546
Bsu36I CCTNAGG 1 cut(s) 409
BsuI GTATCC 1 cut(s) 2219
BsuRI GGCC 6 cut(s) 467, 632, 1079, 1478, 1627, 2417
BtgI CCRYGG 1 cut(s) 1023
BtrI CACGTC 1 cut(s) 1631
BtsI GCAGTG 2 cut(s) 651, 1522
BtsIMutI CAGTG 4 cut(s) 651, 1522, 2010, 2957
BveI ACCTGC 1 cut(s) 2878
Cac8I GCNNGC 4 cut(s) 1502, 1685, 2140, 3019
CaiI CAGNNNCTG 2 cut(s) 2470, 2887
CfoI GCGC 2 cut(s) 2597, 2609
Cfr13I GGNCC 3 cut(s) 1077, 2416, 2858
Csp6I GTAC 5 cut(s) 554, 714, 2269, 2537, 3082
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviQI GTAC 5 cut(s) 554, 714, 2269, 2537, 3082
DraI TTTAAA 4 cut(s) 456, 637, 1617, 1956
DriI GACNNNNNGTC 1 cut(s) 1336
EaeI YGGCCR 3 cut(s) 465, 1476, 1625
Eam1104I CTCTTC 6 cut(s) 130, 365, 844, 2562, 2936, 3169
Eam1105I GACNNNNNGTC 1 cut(s) 1336
EarI CTCTTC 6 cut(s) 130, 365, 844, 2562, 2936, 3169
Eco105I TACGTA 1 cut(s) 2258
Eco130I CCWWGG 1 cut(s) 1023
Eco147I AGGCCT 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 2637
Eco32I GATATC 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 2858
Eco57I CTGAAG 3 cut(s) 21, 2492, 2917
Eco81I CCTNAGG 1 cut(s) 409
EcoRI GAATTC 3 cut(s) 789, 945, 2031
EcoRII CCWGG 2 cut(s) 1473, 2626
EcoRV GATATC 1 cut(s) 365
EcoT14I CCWWGG 1 cut(s) 1023
EcoT22I ATGCAT 1 cut(s) 1542
ErhI CCWWGG 1 cut(s) 1023
FalI AAGNNNNNCTT 8 cut(s) 680, 712, 1328, 1360, 2327, 2359, 2721, 2753
FaqI GGGAC 1 cut(s) 2481
FauI CCCGC 1 cut(s) 2590
FauNDI CATATG 1 cut(s) 2849
FbaI TGATCA 1 cut(s) 2346
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 4 cut(s) 812, 855, 1233, 1560
Fsp4HI GCNGC 4 cut(s) 812, 855, 1233, 1560
FspBI CTAG 8 cut(s) 858, 1149, 1244, 1815, 1907, 2157, 2789, 2834
GlaI GCGC 2 cut(s) 2596, 2608
GluI GCNGC 4 cut(s) 812, 855, 1233, 1560
GsaI CCCAGC 2 cut(s) 818, 2241
GsuI CTGGAG 1 cut(s) 1700
HaeIII GGCC 6 cut(s) 467, 632, 1079, 1478, 1627, 2417
HapII CCGG 3 cut(s) 477, 724, 1552
HhaI GCGC 2 cut(s) 2597, 2609
Hin6I GCGC 2 cut(s) 2595, 2607
HinP1I GCGC 2 cut(s) 2595, 2607
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 3 cut(s) 387, 1863, 2336
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 3 cut(s) 477, 724, 1552
HphI GGTGA 5 cut(s) 191, 445, 985, 1036, 1609
Hpy166II GTNNAC 8 cut(s) 520, 573, 667, 716, 1669, 2309, 3068, 3210
Hpy188III TCNNGA 9 cut(s) 164, 244, 740, 794, 936, 1414, 1430, 1717, 2653
Hpy8I GTNNAC 8 cut(s) 520, 573, 667, 716, 1669, 2309, 3068, 3210
Hpy99I CGWCG 2 cut(s) 2319, 2878
HpyCH4III ACNGT 2 cut(s) 1186, 2842
HpyCH4IV ACGT 6 cut(s) 522, 1053, 1630, 1951, 2257, 2876
HpyF10VI GCNNNNNNNGC 2 cut(s) 678, 1815
HpySE526I ACGT 6 cut(s) 522, 1053, 1630, 1951, 2257, 2876
HspAI GCGC 2 cut(s) 2595, 2607
KpnI GGTACC 1 cut(s) 2540
Ksp22I TGATCA 1 cut(s) 2346
KspAI GTTAAC 1 cut(s) 520
LguI GCTCTTC 1 cut(s) 844
LmnI GCTCC 3 cut(s) 17, 483, 2117
Lsp1109I GCAGC 4 cut(s) 823, 866, 1244, 1571
LweI GCATC 7 cut(s) 38, 439, 1295, 1423, 1527, 1546, 2485
MaeI CTAG 8 cut(s) 858, 1149, 1244, 1815, 1907, 2157, 2789, 2834
MaeII ACGT 6 cut(s) 522, 1053, 1630, 1951, 2257, 2876
MaeIII GTNAC 9 cut(s) 598, 748, 1251, 1517, 1883, 2152, 2318, 2706, 2793
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 4 cut(s) 43, 166, 1801, 2546
MhlI GDGCHC 3 cut(s) 488, 1198, 3116
MlsI TGGCCA 3 cut(s) 467, 1478, 1627
MluNI TGGCCA 3 cut(s) 467, 1478, 1627
MlyI GAGTC 8 cut(s) 120, 496, 554, 578, 1707, 1786, 2234, 2407
MmeI TCCRAC 4 cut(s) 32, 1488, 2387, 3102
Mox20I TGGCCA 3 cut(s) 467, 1478, 1627
Mph1103I ATGCAT 1 cut(s) 1542
MroXI GAANNNNTTC 1 cut(s) 789
MscI TGGCCA 3 cut(s) 467, 1478, 1627
MslI CAYNNNNRTG 3 cut(s) 1291, 2438, 2451
Msp20I TGGCCA 3 cut(s) 467, 1478, 1627
MspA1I CMGCKG 2 cut(s) 814, 2237
MspI CCGG 3 cut(s) 477, 724, 1552
MspR9I CCNGG 5 cut(s) 478, 724, 1475, 1553, 2628
MunI CAATTG 1 cut(s) 231
MvaI CCWGG 2 cut(s) 1475, 2628
MvnI CGCG 1 cut(s) 2597
MwoI GCNNNNNNNGC 2 cut(s) 678, 1815
NciI CCSGG 3 cut(s) 478, 724, 1553
NcoI CCATGG 1 cut(s) 1023
NdeI CATATG 1 cut(s) 2849
NlaIV GGNNCC 1 cut(s) 2538
NmuCI GTSAC 4 cut(s) 748, 1251, 1517, 1883
NsiI ATGCAT 1 cut(s) 1542
NspV TTCGAA 2 cut(s) 585, 783
PaqCI CACCTGC 1 cut(s) 2878
PceI AGGCCT 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 844
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 2277, 2311
PdmI GAANNNNTTC 1 cut(s) 789
PfeI GAWTC 7 cut(s) 422, 736, 1070, 1112, 1469, 1930, 2884
PflFI GACNNNGTC 1 cut(s) 2313
PfoI TCCNGGA 1 cut(s) 1551
PkrI GCNGC 4 cut(s) 813, 856, 1234, 1561
Ple19I CGATCG 1 cut(s) 133
PleI GAGTC 8 cut(s) 120, 496, 554, 577, 1707, 1785, 2233, 2406
PpsI GAGTC 8 cut(s) 120, 496, 554, 577, 1707, 1785, 2233, 2406
Ppu21I YACGTR 1 cut(s) 2258
PsiI TTATAA 1 cut(s) 2673
Psp1406I AACGTT 1 cut(s) 1053
Psp6I CCWGG 2 cut(s) 1473, 2626
PspFI CCCAGC 2 cut(s) 814, 2237
PspGI CCWGG 2 cut(s) 1473, 2626
PspN4I GGNNCC 1 cut(s) 2538
PspPI GGNCC 3 cut(s) 1077, 2416, 2858
PstI CTGCAG 1 cut(s) 2892
PstNI CAGNNNCTG 2 cut(s) 2470, 2887
PsuI RGATCY 4 cut(s) 43, 166, 1801, 2546
PsyI GACNNNGTC 1 cut(s) 2313
PvuI CGATCG 1 cut(s) 133
PvuII CAGCTG 2 cut(s) 814, 2237
RsaI GTAC 5 cut(s) 555, 715, 2270, 2538, 3083
RsaNI GTAC 5 cut(s) 554, 714, 2269, 2537, 3082
RseI CAYNNNNRTG 3 cut(s) 1291, 2438, 2451
SapI GCTCTTC 1 cut(s) 844
SatI GCNGC 4 cut(s) 812, 855, 1233, 1560
Sau96I GGNCC 3 cut(s) 1077, 2416, 2858
SchI GAGTC 8 cut(s) 120, 496, 554, 578, 1707, 1786, 2234, 2407
ScrFI CCNGG 5 cut(s) 478, 724, 1475, 1553, 2628
SduI GDGCHC 3 cut(s) 488, 1198, 3116
SfaNI GCATC 7 cut(s) 38, 439, 1295, 1423, 1527, 1546, 2485
SfcI CTRYAG 3 cut(s) 1386, 2838, 2888
SfuI TTCGAA 2 cut(s) 585, 783
SinI GGWCC 1 cut(s) 2858
SmiMI CAYNNNNRTG 3 cut(s) 1291, 2438, 2451
SmlI CTYRAG 1 cut(s) 1925
SmoI CTYRAG 1 cut(s) 1925
SnaBI TACGTA 1 cut(s) 2258
SseBI AGGCCT 1 cut(s) 632
SsiI CCGC 3 cut(s) 2597, 3093, 3139
SspI AATATT 1 cut(s) 2023
SspMI CTAG 8 cut(s) 858, 1149, 1244, 1815, 1907, 2157, 2789, 2834
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 5 cut(s) 476, 722, 1473, 1551, 2626
StyI CCWWGG 1 cut(s) 1023
TaaI ACNGT 2 cut(s) 1186, 2842
TaiI ACGT 6 cut(s) 525, 1056, 1633, 1954, 2260, 2879
TatI WGTACW 1 cut(s) 3081
TfiI GAWTC 7 cut(s) 422, 736, 1070, 1112, 1469, 1930, 2884
TscAI CASTG 4 cut(s) 658, 1522, 2017, 2957
TseFI GTSAC 4 cut(s) 748, 1251, 1517, 1883
TseI GCWGC 4 cut(s) 811, 854, 1232, 1559
Tsp45I GTSAC 4 cut(s) 748, 1251, 1517, 1883
TspGWI ACGGA 3 cut(s) 941, 2525, 2765
TspRI CASTG 4 cut(s) 658, 1522, 2017, 2957
Tth111I GACNNNGTC 1 cut(s) 2313
VpaK11BI GGWCC 1 cut(s) 2858
XcmI CCANNNNNNNNNTGG 1 cut(s) 2530
XmiI GTMKAC 1 cut(s) 666
XmnI GAANNNNTTC 1 cut(s) 789
XspI CTAG 8 cut(s) 858, 1149, 1244, 1815, 1907, 2157, 2789, 2834
Zsp2I ATGCAT 1 cut(s) 1542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.