Rh5CG438200

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000085
Physical Location & Seq
Reverse (-)
60714133 .. 60761820
47688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG438200.1

Sequence Viewer

Length: 4818 bp
ATGGCCTTGGAATCCCCAACGGCCTCTGCATCAATTCCTACATCAGTTGAAGCATCGGCTCCTGGGTGGAAGTATGAGGTGTTTTTGAGTTTCAGGAGTGAAGACACTCGTTTGTACAATGAATTGTACAATGAATTGCAAAACAGAGAAATCAAGACATTCATGGATATCGACGGAACTAGTTTGGATAGAGGAGAGGGGATTTCTCAAAGTATCGTAGAGGCCATTCAAGAATCAAGATTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCCAGTTGGTGTTTGGATGAACTCACACAGATTATGCAGTGCATGGAAAATAAGGAAACAATTCTGCCAGTTTTCTATCACGTGGATCCCTCTGAAGTGCGCTCTCAAAGGGGGGTGTGCGCAGAGGCCTTGGCTGCACATGAAGAGCAGTTTAAAGATCAAAAAGAGAGGCTGATGAAATGGAGGGACGCCTTGAACAAAATCAGCAGCATCTTTGGCTGGGTATGTGGGAGTTCTGAGCCAGAAGGACAGATTGTCCAACAAATCGCGGAATTTGTCTGCAGCAAACCTATTGAAATTAAGTCGACGGAAGATATTGAAACTCATGGAACTTCTCCTCGCTGGAAGTATGATGTGTTTATAAATTTCAGGGGTGAGGACACTTGTAAGGGTTTTCTGTCCCATTTACTAGCTGAATTGCAAAAGAAACCAGCACACCTGAACACAGTAAAGAATGATGAACCACTTCCAAAAGGGCGGAATATATCTTCGTATCTCCTAAACGCCATAGAAGAGGTGAGGTTTGCCATTGTTGTTCTTTCGCAAGACTTCGCTTCTTCATCATGGTGCCTAGAGGAACTAGCAAAGATTTTTGAATGCATGGGAAACCAAAATAGAATTCTGCCAATCTTTTATCATGTGGATCCCTCTGAGGTGCGACATCAAAGTGGGAGTTTTGGAGATGCCTTCGCTGTACATGAAGAAAGATTTCGGGATAATCATGAGAAGGTCAGGCGGTGGAGACGTGTTTTATCAATGGTAGCCAACATCCCTGGATGGACTTCAGAAAAATTTGAGTATGAAGGAGAACTTATCAAAGACGTTGCGCAAGCTATGTGCAGTAGACTAGTTCAGTCTACAGAGTCGACAGAAGATTTTGAAGCAACAGGAGATGTCATGGACCGAACTCCTCCCCAGCCTGACCCAGCTCCTGTGTGGAAGAATGATGTGCTTTCGGGTTTCGGGATGGAAGACACTCGCAGGGGTTTTGTAGCCCATTCAGACCATGAATTGCCAAACACAGGACTAAGTAAAACATTCAAGGATGAAGACCAGCTTGAAAAAGGGGTAGCCCTCCCCTCTGCATCTCTTTCCTCAACTGAACCAAGTCCTCAACCAGCTGTTGGGCAGAAGAATGATGAGTTTTTGGGTTTCAGGAGAAACGACAATCATAGGGGTTTTGTCACCCATTCAAGTCATGAAATACAAAAGACCAGGGTGACTAAGACATTCACCGATGAAGAACATCTTGAAAAAGGGATAGCTGCGTCTCCGCCTTCATCAGCTGAACGAGATCCTCGGCCGAAGTACGATGTGTTTTTGAGTTTCAGGGGGGAAGACACTCGTAGGGGTTTTATAGCCCATTTATACCGTGAATTGCAAAACACCAGAGTGATTAAAACTTTCAAGGATGACAGGCAACTAGAAAAAGGGACGGTCATTGCTTCAAATCTCCTAACGGCAATCGAAGAATCAAGATTTGCGATCATTGTGTTGTCAAAAAATTATGCTTCTTCTTCATGGTGTTTGGATGAACTTGCAAAGATTTTCCAATGCATGAAAGATAGGAACACAATTTTCCCAATTTTTTATGAGGTGGAACCCACTGATGTACGCCATCAAAAGACAGAGACGAGGTTTGGAAAGGCTTTCATTAAGCAGCACGAAACTCATGGACAAGACAACGAGAAGATGAAGCAATGGAGTGCTGCTTTAGAAGGAGTGGCTGGAATCGCTGGGTTCCCGTCAACTAAGTATCAGACGGACATAGAACTAATCGAAGACGTTGTGAAACATTTGTTGAGCAAAGTACTTCAAGCACCCACCGGAAATTTTGAAACATTTAAAGCGACAAGGCGAGCAATGGATGACGTCATGGAGGTATTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGGTGGCGTTGGAAAGACGACGATGGTCAAACATGTTAGTGCACAAGCCCAGAAAGATGGGATTTTTGATCATGTGATTATGGTTGTCGTATCCAAAAGCCCTGACTTGCAAGAAATTCGAGGCACATTGGCAGGTTCGCTGGCGATCATGAGGGGAAATAGGATGCTTATAATATTGGACGACATTTGGGAGAGTTTTGAACTCTCGAGCATTGGAATTCCCAGTCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTCACCACAAGGAAAAGCAATGTCTGCGATTCTATGGGGTGCCATGCCCAGATCACTCTCAATGTTCTGTCAGAGGAAGACTCTTGGAAGTTATTCATGGAGACGTCGGGGAAGTCCTTTCATGAATCTACCATTTCCTATGATGAAGCGAGAAAGGTAGCTCGAGAATGCTGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACATGGACGAATGGAATGAAGCAGTTGTAACAGCATTTGGAGATGACTGCTTGAACAAATGGAATGAAACTGCTCCACCTGTCAACTTCGAAGCTGAGGAAGACGCATTTCATTCATTGGCATCATCAGATGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCCGAAAATTATGATATCCCAATTGAAGATCTGCTCAAGTATGGGATTGGAAAAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTCAAGTACCTCAAAGTTTCTAGATTGCTTTTAGACGGTAGAAAGAAGGGATGCGTAAGGATGACTGATGGCATTCGAAATCTGGCCATGTCCATTGCGTTGTCCAAAGAAGGCGATGGGTTTTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGTGCAAGAAATCACTTCCAGGAACTTGAATTGCCCAATAGATGCCCATGAAGCCTACTCAACAATCTCACTCATGAGAAACAAGCTTCACAAGCTTCCCGAATATTTGGTATGTTCAAAACTCCAAATTTTATTACTACAAAGTAATGCCGGAATATGTGACATCCCAGAAACCTTTTTGCAAAGTCCTAATCCGCTAAGGGTCTTTGATCTTAGCTTCACTGGTGTTTCGTCACTACCTTCATCATTCAGTTTCCTAACCAACCTCCAAACTTTGAATTTGGATTCTTGCAAGAATTTAAGTAACATATCCGTTCTAGGAAAACTTAATAAGAAACTTAAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAACTTTCCAAAACAAATAGGAGATTTGACCAGTCTAAGGATGTTGGACGTCACTGGTAGATATCTTGAAACTATCCCATCTAAAGTGATATCAAGGTTGTATAGATTAGAAGAACTGCACATGAAATTTCAGTTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGATGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGAAGTTTACATATCTGATGCCGCATGCTTGCCTAAAAGTGTTGAGTACATGCCGAATTGGGCAGAATTTGATATATGCATCAGCAGACACGTCCCTTGCATGGCGCTCCCTCAACATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAGAAGCTTACCGTATTGGTTTATCAGCGCTGTGACAGAGAAGGTGGAGAAGCTAGAGTACACAGATTGCGAAGACTTGAATGACATCGTTAGGGAATATGACCATGGCAGATTACTTGCACTGAAATATCTCTCTGTGAACGGGAACCGTAAGAACTTGAAAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTTTTTGAGAACTTGGAAGAGTTGCATCTGCACCGCTTATATTCCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGATCTCTACGCAAGCTCAGGTTCTTGAAAGTGCAAACTTGTCGTAGCTTGGTGAATGCACTTTTACCATCAACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTGTAAATCTGGAAGGAATGAAATATGTATTTGGATCTGATGAAGGGCTTGATCCAGAAAATATTTTCTTGAGGAAATTGAGGGAGATGAGATTGTCTGCTCTATACGAACTAGTAAGCATATGTAATGGTCCTGCTCCGCGTGTAATCTTCCAGAATCTTAAAGTTTTGGCAATTTGCGAGTGCAAGGAATTGAAAAATCTCTTCACAATAGATGTAGTTGAATGTCTTTGTCAGTTGGAAGACCTTTCCATAGAGCACTGTCCTAGCTTGGACAGAGTCATTGAAGCAAGCAATAGCTTGAACAGCAAGATTAAACTTCTGAAGTTGAAGAAGTTAGCTTTGATAGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAGGTGGTACTATTGAAACTGAGTGCCCTTCATTGGAATACTTGTATGTGGAGAAGTGCCTCCAGTTTTCGTATTTTGCTTACCACTTAGACAACAAGAACCAAGTCCACTTCAATGATAAACAACATTTCAGTTCTTTAGTGAAAAGGTGGGAGGAAGTGCATAATCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1605

Amino Acids

182.11

Weight (kDa)

5.57

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 191 1.3e-43 TIR domain
TIR_2 PF13676 27 - 122 7.3e-17 TIR domain
TIR PF01582 208 - 386 2.7e-47 TIR domain
TIR_2 PF13676 211 - 308 3.5e-09 TIR domain
TIR PF01582 528 - 702 7.3e-48 TIR domain
TIR_2 PF13676 531 - 627 4.1e-14 TIR domain
NB-ARC PF00931 713 - 777 2.3e-09 NB-ARC domain
NB-ARC PF00931 783 - 857 9.2e-16 NB-ARC domain
LRR_14 PF23598 1114 - 1205 2.7e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 1363 - 1509 4.6e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1495 - 1570 4.6e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 638, 2372
AatII GACGTC 3 cut(s) 2151, 2570, 3540
Acc16I TGCGCA 2 cut(s) 397, 1104
Acc36I ACCTGC 1 cut(s) 2324
AccB1I GGYRCC 2 cut(s) 843, 2502
AccB7I CCANNNNNTGG 1 cut(s) 1400
AccI GTMKAC 4 cut(s) 581, 1120, 1133, 1142
AccII CGCG 2 cut(s) 545, 4405
AciI CCGC 8 cut(s) 545, 754, 1012, 1550, 3311, 3738, 4108, 4403
AclWI GGATC 8 cut(s) 356, 369, 914, 927, 1565, 4162, 4306, 4310
AcoI YGGCCR 3 cut(s) 1577, 3042, 3113
AcuI CTGAAG 4 cut(s) 390, 1044, 4607, 4662
AcvI CACGTG 1 cut(s) 358
AcyI GRCGYC 4 cut(s) 465, 2148, 2567, 3537
AfeI AGCGCT 1 cut(s) 3901
AfiI CCNNNNNNNGG 5 cut(s) 1298, 1400, 3525, 3817, 4675
AflII CTTAAG 2 cut(s) 3455, 3485
AflIII ACRYGT 3 cut(s) 1021, 2233, 3805
AhdI GACNNNNNGTC 1 cut(s) 530
AhlI ACTAGT 3 cut(s) 179, 1123, 4375
AjiI CACGTC 2 cut(s) 1022, 3808
AjnI CCWGG 5 cut(s) 61, 1048, 1490, 3134, 4150
AjuI GAANNNNNNNTTGG 4 cut(s) 879, 911, 2813, 2845
AleI CACNNNNGTG 1 cut(s) 1667
Alw21I GWGCWC 4 cut(s) 2245, 2950, 4524, 4626
Alw26I GTCTC 6 cut(s) 1012, 1551, 1901, 2558, 2645, 4231
Alw44I GTGCAC 1 cut(s) 2241
AlwI GGATC 8 cut(s) 356, 369, 914, 927, 1565, 4162, 4306, 4310
AlwNI CAGNNNCTG 1 cut(s) 1208
Ama87I CYCGRG 2 cut(s) 2407, 2625
Aor51HI AGCGCT 1 cut(s) 3901
AoxI GGCC 7 cut(s) 3, 21, 222, 402, 1577, 3042, 3113
ApaLI GTGCAC 1 cut(s) 2241
ApeKI GCWGC 9 cut(s) 410, 483, 558, 1541, 1936, 1985, 2660, 2663, 2841
Asp700I GAANNNNTTC 4 cut(s) 336, 741, 984, 2555
AspLEI GCGC 5 cut(s) 378, 398, 1105, 3823, 3902
AspS9I GGNCC 2 cut(s) 1177, 4394
AsuHPI GGTGA 8 cut(s) 662, 805, 1453, 1501, 1507, 2458, 4151, 4216
AsuII TTCGAA 2 cut(s) 2775, 3034
AvaI CYCGRG 2 cut(s) 2407, 2625
AvaII GGWCC 2 cut(s) 1177, 4394
BaeGI GKGCMC 2 cut(s) 2245, 4670
BalI TGGCCA 2 cut(s) 3044, 3115
BamHI GGATCC 2 cut(s) 361, 919
BanI GGYRCC 2 cut(s) 843, 2502
BarI GAAGNNNNNNTAC 2 cut(s) 3914, 3946
BbrPI CACGTG 1 cut(s) 358
BbsI GAAGAC 9 cut(s) 108, 1254, 1332, 1620, 2064, 2547, 2793, 3951, 4512
Bbv12I GWGCWC 4 cut(s) 2245, 2950, 4524, 4626
BbvCI CCTCAGC 1 cut(s) 2781
BbvI GCAGC 9 cut(s) 397, 495, 570, 1528, 1948, 1972, 2650, 2672, 2828
BceAI ACGGC 2 cut(s) 36, 1752
BcgI CGANNNNNNTGC 4 cut(s) 2300, 2334, 4175, 4209
BciT130I CCWGG 5 cut(s) 63, 1050, 1492, 3136, 4152
BciVI GTATCC 1 cut(s) 2302
BclI TGATCA 2 cut(s) 2170, 2269
BcoDI GTCTC 6 cut(s) 1012, 1551, 1901, 2558, 2645, 4231
BcuI ACTAGT 3 cut(s) 179, 1123, 4375
BfmI CTRYAG 2 cut(s) 556, 1134
BfoI RGCGCY 2 cut(s) 3824, 3903
BfrI CTTAAG 2 cut(s) 3455, 3485
BfuAI ACCTGC 1 cut(s) 2324
BfuI GTATCC 1 cut(s) 2302
BglII AGATCT 2 cut(s) 2878, 4612
BmcAI AGTACT 1 cut(s) 2088
Bme1390I CCNGG 5 cut(s) 63, 1050, 1492, 3136, 4152
Bme18I GGWCC 2 cut(s) 1177, 4394
BmeRI GACNNNNNGTC 1 cut(s) 530
BmeT110I CYCGRG 2 cut(s) 2407, 2625
BmgBI CACGTC 2 cut(s) 1022, 3808
BmgT120I GGNCC 2 cut(s) 1177, 4394
BmiI GGNNCC 8 cut(s) 60, 363, 845, 921, 1878, 2018, 2504, 4019
BmrFI CCNGG 5 cut(s) 63, 1050, 1492, 3136, 4152
BmrI ACTGGG 1 cut(s) 2418
BmuI ACTGGG 1 cut(s) 2418
BoxI GACNNNNGTC 1 cut(s) 2225
BpiI GAAGAC 9 cut(s) 108, 1254, 1332, 1620, 2064, 2547, 2793, 3951, 4512
BplI GAGNNNNNCTC 2 cut(s) 2528, 2560
BpmI CTGGAG 1 cut(s) 4688
Bpu10I CCTNAGC 3 cut(s) 2781, 3314, 4169
Bpu14I TTCGAA 2 cut(s) 2775, 3034
BpuEI CTTGAG 3 cut(s) 2870, 4106, 4354
BsaAI YACGTR 1 cut(s) 358
BsaHI GRCGYC 4 cut(s) 465, 2148, 2567, 3537
BsaI GGTCTC 1 cut(s) 2645
BsaJI CCNNGG 8 cut(s) 6, 62, 405, 1048, 1491, 1574, 2185, 3976
BsaWI WCCGGW 2 cut(s) 2102, 4618
Bsc4I CCNNNNNNNGG 5 cut(s) 1298, 1400, 3525, 3817, 4675
Bse3DI GCAATG 5 cut(s) 1716, 1982, 2145, 2488, 3051
BseBI CCWGG 5 cut(s) 63, 1050, 1492, 3136, 4152
BseDI CCNNGG 8 cut(s) 6, 62, 405, 1048, 1491, 1574, 2185, 3976
BseLI CCNNNNNNNGG 5 cut(s) 1298, 1400, 3525, 3817, 4675
BseMI GCAATG 5 cut(s) 1716, 1982, 2145, 2488, 3051
BseMII CTCAG 5 cut(s) 504, 918, 2772, 4183, 4653
BseRI GAGGAG 4 cut(s) 207, 603, 1176, 3122
BseSI GKGCMC 2 cut(s) 2245, 4670
BseX3I CGGCCG 1 cut(s) 1577
BseXI GCAGC 9 cut(s) 397, 495, 570, 1528, 1948, 1972, 2650, 2672, 2828
BseYI CCCAGC 4 cut(s) 495, 1191, 1201, 2012
BsgI GTGCAG 5 cut(s) 396, 1135, 3590, 4088, 4621
Bsh1236I CGCG 2 cut(s) 545, 4405
Bsh1285I CGRYCG 1 cut(s) 1580
BshFI GGCC 7 cut(s) 5, 23, 224, 404, 1579, 3044, 3115
BshNI GGYRCC 2 cut(s) 843, 2502
BsiEI CGRYCG 1 cut(s) 1580
BsiHKAI GWGCWC 4 cut(s) 2245, 2950, 4524, 4626
BsiHKCI CYCGRG 2 cut(s) 2407, 2625
BsiSI CCGG 3 cut(s) 2103, 3267, 4619
BslFI GGGAC 4 cut(s) 476, 661, 1723, 3794
BslI CCNNNNNNNGG 5 cut(s) 1298, 1400, 3525, 3817, 4675
BsmAI GTCTC 6 cut(s) 1012, 1551, 1901, 2558, 2645, 4231
BsmBI CGTCTC 4 cut(s) 1012, 1551, 1901, 2558
BsmFI GGGAC 4 cut(s) 476, 661, 1723, 3794
BsmI GAATGC 4 cut(s) 878, 2636, 3030, 4213
BsnI GGCC 7 cut(s) 5, 23, 224, 404, 1579, 3044, 3115
Bso31I GGTCTC 1 cut(s) 2645
BsoBI CYCGRG 2 cut(s) 2407, 2625
Bsp119I TTCGAA 2 cut(s) 2775, 3034
Bsp1286I GDGCHC 5 cut(s) 2245, 2950, 4524, 4626, 4670
Bsp1407I TGTACA 3 cut(s) 114, 126, 970
Bsp19I CCATGG 1 cut(s) 3976
BspACI CCGC 8 cut(s) 545, 754, 1012, 1550, 3311, 3738, 4108, 4403
BspANI GGCC 7 cut(s) 5, 23, 224, 404, 1579, 3044, 3115
BspCNI CTCAG 5 cut(s) 505, 919, 2773, 4182, 4654
BspFNI CGCG 2 cut(s) 545, 4405
BspHI TCATGA 5 cut(s) 997, 1474, 2349, 2584, 3189
BspLI GGNNCC 8 cut(s) 60, 363, 845, 921, 1878, 2018, 2504, 4019
BspMAI CTGCAG 1 cut(s) 560
BspMI ACCTGC 1 cut(s) 2324
BspPI GGATC 8 cut(s) 356, 369, 914, 927, 1565, 4162, 4306, 4310
BspQI GCTCTTC 1 cut(s) 414
BspT104I TTCGAA 2 cut(s) 2775, 3034
BspT107I GGYRCC 2 cut(s) 843, 2502
BspTI CTTAAG 2 cut(s) 3455, 3485
BspTNI GGTCTC 1 cut(s) 2645
BsrDI GCAATG 5 cut(s) 1716, 1982, 2145, 2488, 3051
BsrGI TGTACA 3 cut(s) 114, 126, 970
BssECI CCNNGG 8 cut(s) 6, 62, 405, 1048, 1491, 1574, 2185, 3976
BssNI GRCGYC 4 cut(s) 465, 2148, 2567, 3537
BssT1I CCWWGG 4 cut(s) 6, 405, 2185, 3976
Bst2UI CCWGG 5 cut(s) 63, 1050, 1492, 3136, 4152
Bst4CI ACNGT 8 cut(s) 724, 1649, 1714, 2182, 2996, 3885, 4022, 4526
Bst6I CTCTTC 4 cut(s) 414, 783, 4086, 4472
BstACI GRCGYC 4 cut(s) 465, 2148, 2567, 3537
BstAFI CTTAAG 2 cut(s) 3455, 3485
BstAPI GCANNNNNTGC 1 cut(s) 2487
BstAUI TGTACA 3 cut(s) 114, 126, 970
BstBAI YACGTR 1 cut(s) 358
BstBI TTCGAA 2 cut(s) 2775, 3034
BstC8I GCNNGC 7 cut(s) 1107, 2136, 2343, 3742, 3746, 4166, 4555
BstDSI CCRYGG 1 cut(s) 3976
BstFNI CGCG 2 cut(s) 545, 4405
BstH2I RGCGCY 2 cut(s) 3824, 3903
BstHHI GCGC 5 cut(s) 378, 398, 1105, 3823, 3902
BstMAI GTCTC 6 cut(s) 1012, 1551, 1901, 2558, 2645, 4231
BstMCI CGRYCG 1 cut(s) 1580
BstMWI GCNNNNNNNGC 9 cut(s) 410, 492, 2009, 2487, 2657, 3167, 3208, 4569, 4810
BstNI CCWGG 5 cut(s) 63, 1050, 1492, 3136, 4152
BstNSI RCATGY 3 cut(s) 2237, 3744, 3769
BstPAI GACNNNNGTC 1 cut(s) 2225
BstSCI CCNGG 5 cut(s) 61, 1048, 1490, 3134, 4150
BstSFI CTRYAG 2 cut(s) 556, 1134
BstSLI GKGCMC 2 cut(s) 2245, 4670
BstUI CGCG 2 cut(s) 545, 4405
BstV1I GCAGC 9 cut(s) 397, 495, 570, 1528, 1948, 1972, 2650, 2672, 2828
BstV2I GAAGAC 9 cut(s) 108, 1254, 1332, 1620, 2064, 2547, 2793, 3951, 4512
BstX2I RGATCY 7 cut(s) 361, 919, 1570, 2878, 4154, 4298, 4612
BstXI CCANNNNNNTGG 1 cut(s) 2258
BstYI RGATCY 7 cut(s) 361, 919, 1570, 2878, 4154, 4298, 4612
BstZI CGGCCG 1 cut(s) 1577
BsuI GTATCC 1 cut(s) 2302
BsuRI GGCC 7 cut(s) 5, 23, 224, 404, 1579, 3044, 3115
BtgI CCRYGG 1 cut(s) 3976
BtgZI GCGATG 1 cut(s) 3087
BtrI CACGTC 2 cut(s) 1022, 3808
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 9 cut(s) 320, 1881, 2178, 3123, 3336, 3540, 3992, 4522, 4638
BveI ACCTGC 1 cut(s) 2324
Cac8I GCNNGC 7 cut(s) 1107, 2136, 2343, 3742, 3746, 4166, 4555
CaiI CAGNNNCTG 1 cut(s) 1208
CciI TCATGA 5 cut(s) 997, 1474, 2349, 2584, 3189
CfoI GCGC 5 cut(s) 378, 398, 1105, 3823, 3902
Cfr13I GGNCC 2 cut(s) 1177, 4394
CseI GACGC 3 cut(s) 473, 1533, 2798
DraI TTTAAA 3 cut(s) 430, 2122, 3708
DriI GACNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 3 cut(s) 1577, 3042, 3113
EagI CGGCCG 1 cut(s) 1577
Eam1104I CTCTTC 4 cut(s) 414, 783, 4086, 4472
Eam1105I GACNNNNNGTC 1 cut(s) 530
EarI CTCTTC 4 cut(s) 414, 783, 4086, 4472
EciI GGCGGA 2 cut(s) 769, 1539
EclXI CGGCCG 1 cut(s) 1577
Eco130I CCWWGG 4 cut(s) 6, 405, 2185, 3976
Eco147I AGGCCT 1 cut(s) 404
Eco31I GGTCTC 1 cut(s) 2645
Eco32I GATATC 4 cut(s) 169, 2866, 3551, 3579
Eco47I GGWCC 2 cut(s) 1177, 4394
Eco47III AGCGCT 1 cut(s) 3901
Eco52I CGGCCG 1 cut(s) 1577
Eco57I CTGAAG 4 cut(s) 390, 1044, 4607, 4662
Eco72I CACGTG 1 cut(s) 358
Eco88I CYCGRG 2 cut(s) 2407, 2625
EcoRI GAATTC 2 cut(s) 894, 2418
EcoRII CCWGG 5 cut(s) 61, 1048, 1490, 3134, 4150
EcoRV GATATC 4 cut(s) 169, 2866, 3551, 3579
EcoT14I CCWWGG 4 cut(s) 6, 405, 2185, 3976
EcoT22I ATGCAT 3 cut(s) 878, 1835, 3797
ErhI CCWWGG 4 cut(s) 6, 405, 2185, 3976
Esp3I CGTCTC 4 cut(s) 1012, 1551, 1901, 2558
FalI AAGNNNNNCTT 8 cut(s) 1071, 1103, 1317, 1349, 1509, 1541, 2813, 2845
FaqI GGGAC 4 cut(s) 476, 661, 1723, 3794
FauNDI CATATG 1 cut(s) 4385
FbaI TGATCA 2 cut(s) 2170, 2269
FblI GTMKAC 4 cut(s) 581, 1120, 1133, 1142
FspI TGCGCA 2 cut(s) 397, 1104
GlaI GCGC 5 cut(s) 377, 397, 1104, 3822, 3901
GsaI CCCAGC 4 cut(s) 499, 1195, 1205, 2016
GsuI CTGGAG 1 cut(s) 4688
HaeII RGCGCY 2 cut(s) 3824, 3903
HaeIII GGCC 7 cut(s) 5, 23, 224, 404, 1579, 3044, 3115
HapII CCGG 3 cut(s) 2103, 3267, 4619
HgaI GACGC 3 cut(s) 473, 1533, 2798
HhaI GCGC 5 cut(s) 378, 398, 1105, 3823, 3902
Hin1I GRCGYC 4 cut(s) 465, 2148, 2567, 3537
Hin6I GCGC 5 cut(s) 376, 396, 1103, 3821, 3900
HinP1I GCGC 5 cut(s) 376, 396, 1103, 3821, 3900
HincII GTYRAC 5 cut(s) 582, 1143, 2025, 2770, 3690
HindII GTYRAC 5 cut(s) 582, 1143, 2025, 2770, 3690
HindIII AAGCTT 3 cut(s) 3200, 3209, 3877
HpaII CCGG 3 cut(s) 2103, 3267, 4619
HphI GGTGA 8 cut(s) 662, 805, 1453, 1501, 1507, 2458, 4151, 4216
Hpy99I CGWCG 4 cut(s) 176, 586, 2224, 2572
HpyCH4III ACNGT 8 cut(s) 724, 1649, 1714, 2182, 2996, 3885, 4022, 4526
HpyCH4IV ACGT 8 cut(s) 357, 1021, 1098, 2061, 2148, 2567, 3537, 3807
HpyF10VI GCNNNNNNNGC 9 cut(s) 410, 492, 2009, 2487, 2657, 3167, 3208, 4569, 4810
HpySE526I ACGT 8 cut(s) 357, 1021, 1098, 2061, 2148, 2567, 3537, 3807
Hsp92I GRCGYC 4 cut(s) 465, 2148, 2567, 3537
HspAI GCGC 5 cut(s) 376, 396, 1103, 3821, 3900
Ksp22I TGATCA 2 cut(s) 2170, 2269
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 5 cut(s) 64, 1210, 2764, 3828, 4405
Lsp1109I GCAGC 9 cut(s) 397, 495, 570, 1528, 1948, 1972, 2650, 2672, 2828
MaeII ACGT 8 cut(s) 357, 1021, 1098, 2061, 2148, 2567, 3537, 3807
MfeI CAATTG 1 cut(s) 2871
MflI RGATCY 7 cut(s) 361, 919, 1570, 2878, 4154, 4298, 4612
MhlI GDGCHC 5 cut(s) 2245, 2950, 4524, 4626, 4670
MlsI TGGCCA 2 cut(s) 3044, 3115
MluNI TGGCCA 2 cut(s) 3044, 3115
MlyI GAGTC 4 cut(s) 1148, 2537, 3850, 4551
MmeI TCCRAC 5 cut(s) 559, 2191, 3513, 3681, 4482
Mox20I TGGCCA 2 cut(s) 3044, 3115
Mph1103I ATGCAT 3 cut(s) 878, 1835, 3797
MroXI GAANNNNTTC 4 cut(s) 336, 741, 984, 2555
MscI TGGCCA 2 cut(s) 3044, 3115
MslI CAYNNNNRTG 5 cut(s) 841, 942, 1667, 2238, 4755
Msp20I TGGCCA 2 cut(s) 3044, 3115
MspA1I CMGCKG 3 cut(s) 1397, 1562, 2663
MspCI CTTAAG 2 cut(s) 3455, 3485
MspI CCGG 3 cut(s) 2103, 3267, 4619
MspR9I CCNGG 5 cut(s) 63, 1050, 1492, 3136, 4152
MunI CAATTG 1 cut(s) 2871
Mva1269I GAATGC 4 cut(s) 878, 2636, 3030, 4213
MvaI CCWGG 5 cut(s) 63, 1050, 1492, 3136, 4152
MvnI CGCG 2 cut(s) 545, 4405
MwoI GCNNNNNNNGC 9 cut(s) 410, 492, 2009, 2487, 2657, 3167, 3208, 4569, 4810
NcoI CCATGG 1 cut(s) 3976
NdeI CATATG 1 cut(s) 4385
NlaIV GGNNCC 8 cut(s) 60, 363, 845, 921, 1878, 2018, 2504, 4019
NmeAIII GCCGAG 1 cut(s) 1555
NmuCI GTSAC 9 cut(s) 1459, 1495, 2176, 2426, 2670, 3275, 3348, 3538, 3904
NsbI TGCGCA 2 cut(s) 397, 1104
NsiI ATGCAT 3 cut(s) 878, 1835, 3797
NspI RCATGY 3 cut(s) 2237, 3744, 3769
NspV TTCGAA 2 cut(s) 2775, 3034
OliI CACNNNNGTG 1 cut(s) 1667
PaeI GCATGC 1 cut(s) 3744
PaeR7I CTCGAG 2 cut(s) 2407, 2625
PagI TCATGA 5 cut(s) 997, 1474, 2349, 2584, 3189
PceI AGGCCT 1 cut(s) 404
PciI ACATGT 1 cut(s) 2233
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 4 cut(s) 878, 2636, 3030, 4213
PdmI GAANNNNTTC 4 cut(s) 336, 741, 984, 2555
PfeI GAWTC 9 cut(s) 11, 233, 1748, 2007, 2492, 2588, 3401, 3460, 4420
PflFI GACNNNGTC 1 cut(s) 4541
PflMI CCANNNNNTGG 1 cut(s) 1400
PfoI TCCNGGA 1 cut(s) 3134
PleI GAGTC 4 cut(s) 1147, 2537, 3850, 4550
PmaCI CACGTG 1 cut(s) 358
PmlI CACGTG 1 cut(s) 358
PpsI GAGTC 4 cut(s) 1147, 2537, 3850, 4550
Ppu21I YACGTR 1 cut(s) 358
PscI ACATGT 1 cut(s) 2233
PshAI GACNNNNGTC 1 cut(s) 2225
PsiI TTATAA 2 cut(s) 638, 2372
Psp6I CCWGG 5 cut(s) 61, 1048, 1490, 3134, 4150
PspCI CACGTG 1 cut(s) 358
PspFI CCCAGC 4 cut(s) 495, 1191, 1201, 2012
PspGI CCWGG 5 cut(s) 61, 1048, 1490, 3134, 4150
PspN4I GGNNCC 8 cut(s) 60, 363, 845, 921, 1878, 2018, 2504, 4019
PspPI GGNCC 2 cut(s) 1177, 4394
PstI CTGCAG 1 cut(s) 560
PstNI CAGNNNCTG 1 cut(s) 1208
PsuI RGATCY 7 cut(s) 361, 919, 1570, 2878, 4154, 4298, 4612
PsyI GACNNNGTC 1 cut(s) 4541
PvuII CAGCTG 3 cut(s) 1397, 1562, 2663
RseI CAYNNNNRTG 5 cut(s) 841, 942, 1667, 2238, 4755
SalI GTCGAC 2 cut(s) 580, 1141
SapI GCTCTTC 1 cut(s) 414
Sau96I GGNCC 2 cut(s) 1177, 4394
ScaI AGTACT 1 cut(s) 2088
SchI GAGTC 4 cut(s) 1148, 2537, 3850, 4551
ScrFI CCNGG 5 cut(s) 63, 1050, 1492, 3136, 4152
SduI GDGCHC 5 cut(s) 2245, 2950, 4524, 4626, 4670
SfcI CTRYAG 2 cut(s) 556, 1134
Sfr274I CTCGAG 2 cut(s) 2407, 2625
SfuI TTCGAA 2 cut(s) 2775, 3034
SinI GGWCC 2 cut(s) 1177, 4394
SlaI CTCGAG 2 cut(s) 2407, 2625
SmiMI CAYNNNNRTG 5 cut(s) 841, 942, 1667, 2238, 4755
SmlI CTYRAG 7 cut(s) 2407, 2625, 2885, 3455, 3485, 4121, 4333
SmoI CTYRAG 7 cut(s) 2407, 2625, 2885, 3455, 3485, 4121, 4333
SpeI ACTAGT 3 cut(s) 179, 1123, 4375
SphI GCATGC 1 cut(s) 3744
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 8 cut(s) 545, 754, 1012, 1550, 3311, 3738, 4108, 4403
SspI AATATT 3 cut(s) 2376, 3221, 4327
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 5 cut(s) 61, 1048, 1490, 3134, 4150
StyI CCWWGG 4 cut(s) 6, 405, 2185, 3976
TaaI ACNGT 8 cut(s) 724, 1649, 1714, 2182, 2996, 3885, 4022, 4526
TaiI ACGT 8 cut(s) 360, 1024, 1101, 2064, 2151, 2570, 3540, 3810
TaqII GACCGA 1 cut(s) 1194
TatI WGTACW 6 cut(s) 114, 126, 970, 2086, 3762, 3930
TauI GCSGC 1 cut(s) 3740
TfiI GAWTC 9 cut(s) 11, 233, 1748, 2007, 2492, 2588, 3401, 3460, 4420
TscAI CASTG 9 cut(s) 320, 1888, 2185, 3123, 3343, 3547, 3999, 4529, 4645
TseFI GTSAC 9 cut(s) 1459, 1495, 2176, 2426, 2670, 3275, 3348, 3538, 3904
TseI GCWGC 9 cut(s) 410, 483, 558, 1541, 1936, 1985, 2660, 2663, 2841
Tsp45I GTSAC 9 cut(s) 1459, 1495, 2176, 2426, 2670, 3275, 3348, 3538, 3904
TspGWI ACGGA 4 cut(s) 189, 599, 2054, 3418
TspRI CASTG 9 cut(s) 320, 1888, 2185, 3123, 3343, 3547, 3999, 4529, 4645
Tth111I GACNNNGTC 1 cut(s) 4541
Van91I CCANNNNNTGG 1 cut(s) 1400
Vha464I CTTAAG 2 cut(s) 3455, 3485
VneI GTGCAC 1 cut(s) 2241
VpaK11BI GGWCC 2 cut(s) 1177, 4394
XbaI TCTAGA 1 cut(s) 2978
XceI RCATGY 3 cut(s) 2237, 3744, 3769
XcmI CCANNNNNNNNNTGG 2 cut(s) 286, 1209
XhoI CTCGAG 2 cut(s) 2407, 2625
XmiI GTMKAC 4 cut(s) 581, 1120, 1133, 1142
XmnI GAANNNNTTC 4 cut(s) 336, 741, 984, 2555
ZraI GACGTC 3 cut(s) 2149, 2568, 3538
ZrmI AGTACT 1 cut(s) 2088
Zsp2I ATGCAT 3 cut(s) 878, 1835, 3797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.