Rmu_ssc0000170.1_g000026

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000170.1
OG0000085
Physical Location & Seq
Reverse (-)
112568 .. 116332
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000170.1_g000026.1.cds

Sequence Viewer

Length: 3453 bp
atggtcacattgtcctacgacctcttttcttttcagtgtggtgtcactggtgtggtttctgcaatctgcttagtttgcttcatcatgactctgtctttccttttttgttcttctcgtcctcgatcaaagccttcgagcaatgcaagtgcttctgcatctcatcccttacctgttagaccagatcctcagctcttggaagattctgaatcaaagcaacctaaggatgatctagtacgcttccatttcttgaagcaacccaagccatggatcacctacacaagcagttcctggaagcatgatgtgtttttgagctttaggggtgaagacacccgccatggtttcgtatctcatttatacgatgaattggaaagcaccggaagaattaaaacattcaaggatgaccaagggctgcaagcagggatgtcaatttctccaagtctcctcagggcgattgaagaatcaaagctggcaattgttgttttgtcgccaaactacgcttcttctccatggtgtttggatgagcttacacatatatttcaattcatggaaggcagagacagaattctgccaattttttatcatgtggatccagctgatgtaagacatcaaagggggagttttgcaacagctttcactaattatgaaagcaagtccaggcaaagcgaagaaaagttgtatcagtggagagctgccttaagacaagtggccaatatctctgggtggaatacaaagaactttgggtccgagagtgagcttgtcaaacgcattgcgaaagatgtcagcagtaaaacaacacttgaagttcaacaacggcccgtcgaaattgatattaccatcccgtccacccgagagtttagaatatttgaatcaacaagacaggccatggatgagctgattaaggcgctgatagatgatgaggtcaccatcgctggggtttatggcatgggaggtgtgggcaagacaaacatggtcagaaatgttgctctacaagcacgaaaaattgggctctttgatcatgtgattgtggctgtcgtatcccaagaccccaactttagaaggattcaacagcaattggcagagcagctcgacttgagattggaggggacggaatctggaacagctgatagattgaaggagaggataatggaaggaaagaggatccttataatcttggacgacatttgggaaaccatagacttgtcacttataggaattcccaaccccgatgagcttaagcgatgcaactctaaagtcctaatcaccacaagaagattggatgtttgccgctccatgaagagccaagcaagttctcttctcagtatactatctaaagaagattcttggaaactttttgtggattgttcgagaatatcttttgatgaatctaccaatatctatcgagcgagggcgatagttagaaaatgtggtggtctgcccgttgcattgaaagcaattgcacgggcacttgaagataaagacatggctgaatggaatatagcagttcaacaattgagggcatctcaaattgccaaagactatgatatccctatcaaagacttgttcaagtatgctatcgggaacggatttcttcgagaagcagccacaatccaagaagccagagatagagcaaattcggtgatcaagtacctcaaagcttccagcttacttttggacagtccaaaggagggatgcgtaaagatgcatgatgttgttcgggatacagccaagtcaattccaaggtatgaggatgatcatggattttgcgttaaagctggttgtggattaaaggagtttcgagggaccactgggcatggagactgctctgccatctcactaatgatgaatgatattgatcggcttcctgaagagttggtatgtccaaaactccagattttgttactacaaaacaatgttcatatagatgagatcccagaatctttttttcgaagttcgaaggcactaagggtcttagatattagcttctctagaatttcatcactacccaggtcatttagtttcctaaccaacctccgagctttgtttttagaccattgcaatgagttggttgacatatcaatattcggaaacttgaagagacttgagattcttagtatgatagaatttcctctcaaggagttaccaaaagagatgggaaatttgaccaatctaaggatgctggacttaagtgggagttacaatattggtatatttccatatagtgtgctgtcaagattgtgtaaattagaagaactgtacatgcaatgtaattctgggaactggggaagcaaaattcatggaggagaagaaactaatgttggtcctgatgagttgattggcttgccacttttaaacaccgtgaagcttcgcatatctgatgcagaatgcttgcctaaaaatcttttctgggattggcatttggttacctttgatatatgtatcagcagagaaccaccatctagagactactcttcagaaccaattttctcacacgatcattcaaggaccctgactcttaacacaacaatcaacagcttaccagattggtttatcaacacagtgacagagaaagcgctgaaactacagtacctaaagtgcaggggattaaagaacattcttgtggaacataacagaggcagattacatggactgaagaatcttgctgtaattggtccacatgagaacttggtagtgttgatggaaacaataacactggtgccaaataaacctgtgtttgagaagttggaagagttgcatctccttggtgtgagttgcctgaaggaattatgtgttggtgaattacccgttgggtctctatgcaatctcaagttattcaaggtagaatattgccatgacttggtaaagacactcttaccatcaaatttgtggcagagactacacaatatggaaaaactaatttgtgaagatatgaatcaaatggagtatgtatttggatccaaagggatggagtcagaagatattatcttgataaaattgagagagatgatattgtggaatctagagaatctaataggcatatgcaatggtcctgctccaaatgcagttttccatagtcttaaaagtttggcagtatatgggtgcaagaaactgaaacatctgttcacatacgatatagctcactgtcttttgcatttggaagacctttcggtgactcagtgttctagcttggacagggttatcgaacctagcaaggaaacagtcaacaagaagatggtttttcctgaattgaaaaacttagctttgtcatatcttccacagctcacaaggttctgcagtagtgaaagtgctaccattgagtgcccttcattggaacacttgcatgtgcaggactgcccccattttacaacctctatttctgacttccacagcgggaatcaattcatccaagtcaatgatctacgacatttgaggtataataaacttattagtcggtaa

Protein Analysis

1150

Amino Acids

130.43

Weight (kDa)

6.65

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1166
AccB1I GGYRCC 1 cut(s) 2713
AccB7I CCANNNNNTGG 2 cut(s) 264, 2854
AccBSI CCGCTC 1 cut(s) 1287
AccI GTMKAC 1 cut(s) 1321
AciI CCGC 3 cut(s) 331, 1285, 3387
AclWI GGATC 9 cut(s) 176, 275, 581, 594, 1153, 1166, 1921, 2946, 2959
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 8 cut(s) 561, 1212, 1630, 1989, 2120, 2155, 2289, 2878
AcuI CTGAAG 4 cut(s) 1884, 2454, 2669, 2795
AfaI GTAC 4 cut(s) 234, 1646, 2255, 2585
AfeI AGCGCT 1 cut(s) 2571
AfiI CCNNNNNNNGG 6 cut(s) 264, 930, 1685, 2169, 2854, 3325
AflII CTTAAG 3 cut(s) 694, 1232, 2182
AjnI CCWGG 3 cut(s) 287, 653, 2003
AjuI GAANNNNNNNTTGG 8 cut(s) 481, 513, 2298, 2330, 2772, 2787, 2804, 2819
AleI CACNNNNGTG 1 cut(s) 50
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
Alw26I GTCTC 7 cut(s) 443, 549, 1809, 2089, 2454, 2814, 2884
AlwI GGATC 9 cut(s) 176, 275, 581, 594, 1153, 1166, 1921, 2946, 2959
AlwNI CAGNNNCTG 1 cut(s) 288
Ama87I CYCGRG 1 cut(s) 846
Aor51HI AGCGCT 1 cut(s) 2571
AoxI GGCC 3 cut(s) 705, 812, 879
ApeKI GCWGC 4 cut(s) 409, 689, 1081, 1598
ApoI RAATTY 8 cut(s) 561, 1212, 1630, 1989, 2120, 2155, 2289, 2878
Asp700I GAANNNNTTC 1 cut(s) 3395
AspLEI GCGC 2 cut(s) 904, 2572
AspS9I GGNCC 7 cut(s) 741, 813, 1800, 2318, 2502, 2669, 3044
AsuHPI GGTGA 7 cut(s) 262, 332, 913, 1252, 1648, 2804, 3178
AsuII TTCGAA 2 cut(s) 1945, 1952
AvaI CYCGRG 1 cut(s) 846
AvaII GGWCC 6 cut(s) 741, 1800, 2318, 2502, 2669, 3044
AxyI CCTNAGG 2 cut(s) 219, 443
BaeGI GKGCMC 2 cut(s) 1465, 3320
BaeI ACNNNNGTAYC 2 cut(s) 2567, 2600
BalI TGGCCA 1 cut(s) 707
BamHI GGATCC 3 cut(s) 586, 1158, 2951
BanI GGYRCC 1 cut(s) 2713
BanII GRGCYC 1 cut(s) 1008
BbsI GAAGAC 2 cut(s) 330, 3162
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 4 cut(s) 396, 676, 1093, 1610
BccI CCATC 9 cut(s) 842, 932, 1835, 2143, 2461, 2689, 2881, 2956, 3223
BceAI ACGGC 1 cut(s) 827
BciT130I CCWGG 3 cut(s) 289, 655, 2005
BciVI GTATCC 2 cut(s) 1045, 1711
BclI TGATCA 3 cut(s) 1012, 1638, 1750
BcoDI GTCTC 7 cut(s) 443, 549, 1809, 2089, 2454, 2814, 2884
BfaI CTAG 6 cut(s) 230, 1986, 2457, 3017, 3180, 3204
BfmI CTRYAG 2 cut(s) 2579, 3289
BfoI RGCGCY 2 cut(s) 905, 2573
BfrI CTTAAG 3 cut(s) 694, 1232, 2182
BfuI GTATCC 2 cut(s) 1045, 1711
BisI GCNGC 5 cut(s) 410, 690, 1082, 1285, 1599
BlsI GCNGC 5 cut(s) 411, 691, 1083, 1286, 1600
Bme1390I CCNGG 3 cut(s) 289, 655, 2005
Bme18I GGWCC 6 cut(s) 741, 1800, 2318, 2502, 2669, 3044
BmeT110I CYCGRG 1 cut(s) 846
BmgT120I GGNCC 7 cut(s) 741, 813, 1800, 2318, 2502, 2669, 3044
BmiI GGNNCC 7 cut(s) 588, 742, 1160, 1801, 2504, 2715, 2953
BmrFI CCNGG 3 cut(s) 289, 655, 2005
BmrI ACTGGG 2 cut(s) 1815, 2287
BmsI GCATC 8 cut(s) 164, 1229, 1526, 1679, 1689, 2163, 2365, 2761
BmuI ACTGGG 2 cut(s) 1815, 2287
BpiI GAAGAC 2 cut(s) 330, 3162
BplI GAGNNNNNCTC 2 cut(s) 1504, 1536
BpmI CTGGAG 1 cut(s) 1871
Bpu10I CCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 2 cut(s) 1945, 1952
BpuEI CTTGAG 4 cut(s) 1111, 2114, 2120, 2807
BsaBI GATNNNNATC 2 cut(s) 1851, 2928
BsaI GGTCTC 1 cut(s) 2814
BsaJI CCNNGG 8 cut(s) 263, 334, 403, 506, 882, 1736, 2003, 2758
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 6 cut(s) 264, 930, 1685, 2169, 2854, 3325
Bse1I ACTGG 4 cut(s) 52, 1810, 2282, 2715
Bse21I CCTNAGG 2 cut(s) 219, 443
Bse3DI GCAATG 6 cut(s) 145, 765, 2050, 2062, 2267, 3046
Bse8I GATNNNNATC 2 cut(s) 1851, 2928
BseBI CCWGG 3 cut(s) 289, 655, 2005
BseDI CCNNGG 8 cut(s) 263, 334, 403, 506, 882, 1736, 2003, 2758
BseJI GATNNNNATC 2 cut(s) 1851, 2928
BseLI CCNNNNNNNGG 6 cut(s) 264, 930, 1685, 2169, 2854, 3325
BseMI GCAATG 6 cut(s) 145, 765, 2050, 2062, 2267, 3046
BseMII CTCAG 4 cut(s) 200, 457, 1330, 3185
BseNI ACTGG 4 cut(s) 52, 1810, 2282, 2715
BseRI GAGGAG 2 cut(s) 431, 2313
BseSI GKGCMC 2 cut(s) 1465, 3320
BseXI GCAGC 4 cut(s) 396, 676, 1093, 1610
BseYI CCCAGC 1 cut(s) 929
BsgI GTGCAG 2 cut(s) 2614, 3362
BshFI GGCC 3 cut(s) 707, 814, 881
BshNI GGYRCC 1 cut(s) 2713
BsiHKCI CYCGRG 1 cut(s) 846
BsiSI CCGG 1 cut(s) 375
BslFI GGGAC 2 cut(s) 1117, 1813
BslI CCNNNNNNNGG 6 cut(s) 264, 930, 1685, 2169, 2854, 3325
BsmAI GTCTC 7 cut(s) 443, 549, 1809, 2089, 2454, 2814, 2884
BsmFI GGGAC 2 cut(s) 1117, 1813
BsmI GAATGC 1 cut(s) 2387
BsnI GGCC 3 cut(s) 707, 814, 881
Bso31I GGTCTC 1 cut(s) 2814
BsoBI CYCGRG 1 cut(s) 846
Bsp119I TTCGAA 2 cut(s) 1945, 1952
Bsp1286I GDGCHC 3 cut(s) 1008, 1465, 3320
Bsp1407I TGTACA 1 cut(s) 2253
Bsp19I CCATGG 4 cut(s) 263, 334, 506, 882
BspACI CCGC 3 cut(s) 331, 1285, 3387
BspANI GGCC 3 cut(s) 707, 814, 881
BspCNI CTCAG 4 cut(s) 199, 456, 1329, 3184
BspHI TCATGA 1 cut(s) 84
BspLI GGNNCC 7 cut(s) 588, 742, 1160, 1801, 2504, 2715, 2953
BspMAI CTGCAG 1 cut(s) 3293
BspPI GGATC 9 cut(s) 176, 275, 581, 594, 1153, 1166, 1921, 2946, 2959
BspQI GCTCTTC 1 cut(s) 1289
BspT104I TTCGAA 2 cut(s) 1945, 1952
BspT107I GGYRCC 1 cut(s) 2713
BspTI CTTAAG 3 cut(s) 694, 1232, 2182
BspTNI GGTCTC 1 cut(s) 2814
BsrBI CCGCTC 1 cut(s) 1287
BsrDI GCAATG 6 cut(s) 145, 765, 2050, 2062, 2267, 3046
BsrGI TGTACA 1 cut(s) 2253
BsrI ACTGG 4 cut(s) 52, 1810, 2282, 2715
BssECI CCNNGG 8 cut(s) 263, 334, 403, 506, 882, 1736, 2003, 2758
BssNAI GTATAC 1 cut(s) 1322
BssT1I CCWWGG 7 cut(s) 263, 334, 403, 506, 882, 1736, 2758
Bst1107I GTATAC 1 cut(s) 1322
Bst2UI CCWGG 3 cut(s) 289, 655, 2005
Bst4CI ACNGT 7 cut(s) 1676, 2253, 2356, 2557, 2583, 3140, 3217
Bst6I CTCTTC 6 cut(s) 1289, 1317, 1860, 2087, 2473, 2739
BstAFI CTTAAG 3 cut(s) 694, 1232, 2182
BstAUI TGTACA 1 cut(s) 2253
BstBI TTCGAA 2 cut(s) 1945, 1952
BstC8I GCNNGC 4 cut(s) 414, 468, 2339, 2387
BstDSI CCRYGG 4 cut(s) 263, 334, 506, 882
BstEII GGTNACC 2 cut(s) 919, 2419
BstH2I RGCGCY 2 cut(s) 905, 2573
BstHHI GCGC 2 cut(s) 904, 2572
BstMAI GTCTC 7 cut(s) 443, 549, 1809, 2089, 2454, 2814, 2884
BstMWI GCNNNNNNNGC 5 cut(s) 75, 259, 989, 1448, 3056
BstNI CCWGG 3 cut(s) 289, 655, 2005
BstNSI RCATGY 2 cut(s) 2260, 3341
BstPI GGTNACC 2 cut(s) 919, 2419
BstSCI CCNGG 3 cut(s) 287, 653, 2003
BstSFI CTRYAG 2 cut(s) 2579, 3289
BstSLI GKGCMC 2 cut(s) 1465, 3320
BstV1I GCAGC 4 cut(s) 396, 676, 1093, 1610
BstV2I GAAGAC 2 cut(s) 330, 3162
BstX2I RGATCY 5 cut(s) 181, 586, 1158, 1926, 2951
BstXI CCANNNNNNTGG 1 cut(s) 2962
BstYI RGATCY 5 cut(s) 181, 586, 1158, 1926, 2951
BstZ17I GTATAC 1 cut(s) 1322
Bsu36I CCTNAGG 2 cut(s) 219, 443
BsuI GTATCC 2 cut(s) 1045, 1711
BsuRI GGCC 3 cut(s) 707, 814, 881
BtgI CCRYGG 4 cut(s) 263, 334, 506, 882
BtgZI GCGATG 2 cut(s) 910, 1252
BtsIMutI CAGTG 8 cut(s) 41, 45, 686, 1803, 2562, 2708, 3136, 3179
Cac8I GCNNGC 4 cut(s) 414, 468, 2339, 2387
CaiI CAGNNNCTG 1 cut(s) 288
CciI TCATGA 1 cut(s) 84
CfoI GCGC 2 cut(s) 904, 2572
Cfr13I GGNCC 7 cut(s) 741, 813, 1800, 2318, 2502, 2669, 3044
Csp6I GTAC 4 cut(s) 233, 1645, 2254, 2584
CviQI GTAC 4 cut(s) 233, 1645, 2254, 2584
DraI TTTAAA 1 cut(s) 2349
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 6 cut(s) 1289, 1317, 1860, 2087, 2473, 2739
EarI CTCTTC 6 cut(s) 1289, 1317, 1860, 2087, 2473, 2739
Eco130I CCWWGG 7 cut(s) 263, 334, 403, 506, 882, 1736, 2758
Eco24I GRGCYC 1 cut(s) 1008
Eco31I GGTCTC 1 cut(s) 2814
Eco32I GATATC 1 cut(s) 1543
Eco47I GGWCC 6 cut(s) 741, 1800, 2318, 2502, 2669, 3044
Eco47III AGCGCT 1 cut(s) 2571
Eco57I CTGAAG 4 cut(s) 1884, 2454, 2669, 2795
Eco81I CCTNAGG 2 cut(s) 219, 443
Eco88I CYCGRG 1 cut(s) 846
Eco91I GGTNACC 2 cut(s) 919, 2419
EcoO109I RGGNCCY 1 cut(s) 2502
EcoO65I GGTNACC 2 cut(s) 919, 2419
EcoRI GAATTC 2 cut(s) 561, 1212
EcoRII CCWGG 3 cut(s) 287, 653, 2003
EcoRV GATATC 1 cut(s) 1543
EcoT14I CCWWGG 7 cut(s) 263, 334, 403, 506, 882, 1736, 2758
EcoT22I ATGCAT 1 cut(s) 1704
EcoT38I GRGCYC 1 cut(s) 1008
ErhI CCWWGG 7 cut(s) 263, 334, 403, 506, 882, 1736, 2758
FalI AAGNNNNNCTT 2 cut(s) 2852, 2884
FaqI GGGAC 2 cut(s) 1117, 1813
FauI CCCGC 2 cut(s) 338, 3380
FauNDI CATATG 1 cut(s) 3035
FbaI TGATCA 3 cut(s) 1012, 1638, 1750
FblI GTMKAC 1 cut(s) 1321
Fnu4HI GCNGC 5 cut(s) 410, 690, 1082, 1285, 1599
FriOI GRGCYC 1 cut(s) 1008
Fsp4HI GCNGC 5 cut(s) 410, 690, 1082, 1285, 1599
FspBI CTAG 6 cut(s) 230, 1986, 2457, 3017, 3180, 3204
GlaI GCGC 2 cut(s) 903, 2571
GluI GCNGC 5 cut(s) 410, 690, 1082, 1285, 1599
GsaI CCCAGC 1 cut(s) 933
GsuI CTGGAG 1 cut(s) 1871
HaeII RGCGCY 2 cut(s) 905, 2573
HaeIII GGCC 3 cut(s) 707, 814, 881
HapII CCGG 1 cut(s) 375
HhaI GCGC 2 cut(s) 904, 2572
Hin6I GCGC 2 cut(s) 902, 2570
HinP1I GCGC 2 cut(s) 902, 2570
HincII GTYRAC 2 cut(s) 2068, 3220
HindII GTYRAC 2 cut(s) 2068, 3220
HindIII AAGCTT 2 cut(s) 1653, 2360
HpaII CCGG 1 cut(s) 375
HphI GGTGA 7 cut(s) 262, 332, 913, 1252, 1648, 2804, 3178
Hpy166II GTNNAC 6 cut(s) 843, 1322, 2068, 2672, 3120, 3220
Hpy188I TCNGA 9 cut(s) 205, 745, 974, 2033, 2084, 2374, 2473, 2971, 3376
Hpy8I GTNNAC 6 cut(s) 843, 1322, 2068, 2672, 3120, 3220
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 8 cut(s) 141, 542, 1050, 1126, 1142, 1948, 2770, 3330
HpyCH4III ACNGT 7 cut(s) 1676, 2253, 2356, 2557, 2583, 3140, 3217
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 259, 989, 1448, 3056
HspAI GCGC 2 cut(s) 902, 2570
Ksp22I TGATCA 3 cut(s) 1012, 1638, 1750
LguI GCTCTTC 1 cut(s) 1289
LmnI GCTCC 2 cut(s) 1292, 3055
Lsp1109I GCAGC 4 cut(s) 396, 676, 1093, 1610
LweI GCATC 8 cut(s) 164, 1229, 1526, 1679, 1689, 2163, 2365, 2761
MaeI CTAG 6 cut(s) 230, 1986, 2457, 3017, 3180, 3204
MbiI CCGCTC 1 cut(s) 1287
MfeI CAATTG 4 cut(s) 471, 1070, 1452, 1508
MflI RGATCY 5 cut(s) 181, 586, 1158, 1926, 2951
MhlI GDGCHC 3 cut(s) 1008, 1465, 3320
MlsI TGGCCA 1 cut(s) 707
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 4 cut(s) 82, 2503, 2975, 3163
MmeI TCCRAC 1 cut(s) 2721
Mox20I TGGCCA 1 cut(s) 707
Mph1103I ATGCAT 1 cut(s) 1704
MroXI GAANNNNTTC 1 cut(s) 3395
MscI TGGCCA 1 cut(s) 707
MslI CAYNNNNRTG 5 cut(s) 50, 1920, 2055, 2615, 3336
Msp20I TGGCCA 1 cut(s) 707
MspA1I CMGCKG 3 cut(s) 593, 1121, 3387
MspCI CTTAAG 3 cut(s) 694, 1232, 2182
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 3 cut(s) 289, 655, 2005
MunI CAATTG 4 cut(s) 471, 1070, 1452, 1508
Mva1269I GAATGC 1 cut(s) 2387
MvaI CCWGG 3 cut(s) 289, 655, 2005
MwoI GCNNNNNNNGC 5 cut(s) 75, 259, 989, 1448, 3056
NcoI CCATGG 4 cut(s) 263, 334, 506, 882
NdeI CATATG 1 cut(s) 3035
NlaIV GGNNCC 7 cut(s) 588, 742, 1160, 1801, 2504, 2715, 2953
NmuCI GTSAC 6 cut(s) 4, 43, 919, 1200, 2557, 3166
NsiI ATGCAT 1 cut(s) 1704
NspI RCATGY 2 cut(s) 2260, 3341
NspV TTCGAA 2 cut(s) 1945, 1952
OliI CACNNNNGTG 1 cut(s) 50
PagI TCATGA 1 cut(s) 84
PciSI GCTCTTC 1 cut(s) 1289
PctI GAATGC 1 cut(s) 2387
PdmI GAANNNNTTC 1 cut(s) 3395
PflFI GACNNNGTC 1 cut(s) 91
PflMI CCANNNNNTGG 2 cut(s) 264, 2854
PfoI TCCNGGA 1 cut(s) 287
PkrI GCNGC 5 cut(s) 411, 691, 1083, 1286, 1600
PleI GAGTC 4 cut(s) 82, 2503, 2974, 3163
PpsI GAGTC 4 cut(s) 82, 2503, 2974, 3163
PpuMI RGGWCCY 1 cut(s) 2502
PsiI TTATAA 1 cut(s) 1166
Psp5II RGGWCCY 1 cut(s) 2502
Psp6I CCWGG 3 cut(s) 287, 653, 2003
PspEI GGTNACC 2 cut(s) 919, 2419
PspFI CCCAGC 1 cut(s) 929
PspGI CCWGG 3 cut(s) 287, 653, 2003
PspN4I GGNNCC 7 cut(s) 588, 742, 1160, 1801, 2504, 2715, 2953
PspPI GGNCC 7 cut(s) 741, 813, 1800, 2318, 2502, 2669, 3044
PspPPI RGGWCCY 1 cut(s) 2502
PstI CTGCAG 1 cut(s) 3293
PstNI CAGNNNCTG 1 cut(s) 288
PsuI RGATCY 5 cut(s) 181, 586, 1158, 1926, 2951
PsyI GACNNNGTC 1 cut(s) 91
PvuII CAGCTG 2 cut(s) 593, 1121
RsaI GTAC 4 cut(s) 234, 1646, 2255, 2585
RsaNI GTAC 4 cut(s) 233, 1645, 2254, 2584
RseI CAYNNNNRTG 5 cut(s) 50, 1920, 2055, 2615, 3336
SapI GCTCTTC 1 cut(s) 1289
SatI GCNGC 5 cut(s) 410, 690, 1082, 1285, 1599
Sau96I GGNCC 7 cut(s) 741, 813, 1800, 2318, 2502, 2669, 3044
SchI GAGTC 4 cut(s) 82, 2503, 2975, 3163
ScrFI CCNGG 3 cut(s) 289, 655, 2005
SduI GDGCHC 3 cut(s) 1008, 1465, 3320
SfaNI GCATC 8 cut(s) 164, 1229, 1526, 1679, 1689, 2163, 2365, 2761
SfcI CTRYAG 2 cut(s) 2579, 3289
SfuI TTCGAA 2 cut(s) 1945, 1952
SinI GGWCC 6 cut(s) 741, 1800, 2318, 2502, 2669, 3044
SmiMI CAYNNNNRTG 5 cut(s) 50, 1920, 2055, 2615, 3336
SmlI CTYRAG 7 cut(s) 694, 1090, 1232, 2099, 2129, 2182, 2822
SmoI CTYRAG 7 cut(s) 694, 1090, 1232, 2099, 2129, 2182, 2822
SsiI CCGC 3 cut(s) 331, 1285, 3387
SspI AATATT 4 cut(s) 861, 2079, 2200, 2843
SspMI CTAG 6 cut(s) 230, 1986, 2457, 3017, 3180, 3204
StyD4I CCNGG 3 cut(s) 287, 653, 2003
StyI CCWWGG 7 cut(s) 263, 334, 403, 506, 882, 1736, 2758
TaaI ACNGT 7 cut(s) 1676, 2253, 2356, 2557, 2583, 3140, 3217
TatI WGTACW 1 cut(s) 2253
TauI GCSGC 1 cut(s) 1287
TscAI CASTG 8 cut(s) 41, 52, 686, 1810, 2562, 2715, 3143, 3179
TseFI GTSAC 6 cut(s) 4, 43, 919, 1200, 2557, 3166
TseI GCWGC 4 cut(s) 409, 689, 1081, 1598
Tsp45I GTSAC 6 cut(s) 4, 43, 919, 1200, 2557, 3166
TspGWI ACGGA 2 cut(s) 1121, 1596
TspRI CASTG 8 cut(s) 41, 52, 686, 1810, 2562, 2715, 3143, 3179
Tth111I GACNNNGTC 1 cut(s) 91
Van91I CCANNNNNTGG 2 cut(s) 264, 2854
Vha464I CTTAAG 3 cut(s) 694, 1232, 2182
VpaK11BI GGWCC 6 cut(s) 741, 1800, 2318, 2502, 2669, 3044
XapI RAATTY 8 cut(s) 561, 1212, 1630, 1989, 2120, 2155, 2289, 2878
XbaI TCTAGA 3 cut(s) 1985, 2456, 3016
XceI RCATGY 2 cut(s) 2260, 3341
XcmI CCANNNNNNNNNTGG 3 cut(s) 1270, 1666, 2880
XmiI GTMKAC 1 cut(s) 1321
XmnI GAANNNNTTC 1 cut(s) 3395
XspI CTAG 6 cut(s) 230, 1986, 2457, 3017, 3180, 3204
Zsp2I ATGCAT 1 cut(s) 1704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.