Rw0G008170

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00301
OG0000085
Physical Location & Seq
Forward (+)
61706 .. 85165
23460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008170.1

Sequence Viewer

Length: 3102 bp
ATGCTGGCTTCAGAAGTAGCTTACTTAGCATTGTATCCTTCTCCTGCTTGCTCAGTCTTTGAGCCTAGGAACAAGGGTAAGATCTACGTGATTGCTGCTAAGATGTCTTCAGGGAAGGTGGTGACTACGAAAAGAACAAAATTCAGCGGCTTCAACGCAATCTCGAACAGAACAGAAAGAGAACTTTTGAATGCCATTGAGGAATCTGTGTGGAACAAATTACGACCTACTGAACATGTGTTTAAAATGTCCATAGGAGATTTTCAAGAATTCGCAGCAACAAAAGAAGCCATTGATGAGGTCATGAATGCGCTAAAAAATGATGATGCCGCCACTACTATTGGAGTCTACGGCATGGGGGGCGTTGGAAAAACGACAATGGTGAAATATGTTGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATCATGGCTATCGTATCCCAAAACCCCGACTTGATGAAAATTCAACAGACATTTGCAGAACTGCTGGGCTTTGAATTGGTGGAGAAGACAGAAATTGGAAGAGCCATTAAATTGAAGGAGAAGATAATGAGAGGAACTGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCGGACATTGGAATTCCAAGCCACAACGAACTTCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAATTGAGTGTTTGCCGTTCCATGGATTGCCATGCAAACATACATCTCAATATCTTATCAGAAGAAGATTCTCGGAGCTTATTTGTGAAGGAAGCAAGGAAGTACCCTCTTTACAAATCATCCAATTTCTATGATGTAGCGAGTGAGGTGGCTAGAGAATGCGCTGGTCTACCTATTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGGTGAAGGTTTGGATAGATGGAAGGAAGCTGCTCGACGACTAAAAGCGTCTCAACCTCCCATCCTTGAAGACGAGGAAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAAACTGATGTTTCGAAATCATGCTTCTTGCTTTGTTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTATGGAATTGGCAAAGGAATGTTTCAAGATTCCGACATGCAAGAAGCCCGCGCCACAACAGATTTAGCGGTGGAGGCCCTTAAAGATTCTAGCTTGCTTTTGGACGCTAGAGTTGACGGATGCATAAAGATGCATGATGTCATTCGGGATATGGCAATGTTAATTTCGTCTTCTGAAGGCCAGCGGTTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACTGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCAAGCTACCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAAGACAATGCTTCATTAAATGATATCCCAAACTCTTTCTTCCAAAGTCAGAAAGAATTAAGAGTCTTAGATCTTAGCCGCACTGAAATTTCATTACTGCCGCAATCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATGACTGCTGTCATTGGGAAATAATTGACATTTCTGTAATTGGGAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGAAAGAATTTCCCAGAGAAATAGGACAGTTGACCAATCTAAGGATGTTGGATGTCAGTGATGAACCTGATCCACTGAGAATGAGCTTGCATCAACGTCTTGCCACAATTCCATCTAAAGTGATATCGAAGTTGCATAAATTAGAAGAACTGTACATGCAATATTGTGGCTTTTGGGACTGGGGGAGTAAAATTGATCGAGAAGGAGAAGAAACTAATATTGGTTTTGATGAATTAGCTGGTTTATCATATTTGAGAATTTTGAAAGTTTGCATGTCAGATGCAAATTGCATTCCTAAAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTCGATATTAGTATTTGCAGCAGCAGTAATAGACTGATAGATGATATGAACTTTCAATCTGGTCATAATTGTAGAACCTTGAGTCTTAATAGTACTGAAACAACCATAGGAACCTTTCCGAACTGGTTTGTCAACGCTGGGGTAAAGAATACTGAGAGGCTGTGTTATAAAAATTGCAATGGGTTAAGTAACATCCTTGTGGAGTATGACCGTGGGAGGTTACATGGACTGAAAGTTCTCTCTGTAAGAAGTTGCTGTGAGAACTTGAAAGAGTTGATGAATGCAATAACATGTGTTCCTGATAAGCCTGTGTTTGAAAACTTGGAACAGTTGTATCTGTTTTGCCTGGATGAACTGAAGCAGTTATGTGTTGGTGAGTTACCGCCTGGATCTCTCTGCAGTCTGAAATTATTGGAGGTCCGTTGGTGTCGTAACTTGGGGAATGTTCTGTTGCCATCAAAATTGTTGCAGAAACTATCAAATCTAGAAAAACTATTCTGTGATACAAGTGGAGTAGAATATGTGTTTGGATGTGAAGGATTTGAGTTGGATCAAACAAACCTGAGAGAGATGGAGTTGTTGGATCTACAAGTAGTAAGAAGCATGTGTAATGGTCCTGCTCCACGTGGAATGTTCCAGGCTCTAAAGGAATTGAACATTCATAATTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAGACCTTTTAGTAACCTACTGCCCTGTTTTGGAAAGAGTAATCGAAGCAAGGCCGGAAACAGTGAACAACAAGAAGACCGTTCTTCCAAAATTGAAGAACTTACGGTTGCTACATCTTCCAATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAACCTTGAGTCTAAAGGGCTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCCTTTTCAATGATGATCGGCTGGTTCCCAAATTGCAAGTGAAAGAACTTCTCCGATCATTTTATGGCCCATTCGACGATCTAAAATGCGAGAGGAAGGTAACTTCGTTAAGGTACGCCTCTGTGTCCTATACTTACTTCTATTAA

Protein Analysis

1033

Amino Acids

117.1

Weight (kDa)

6.16

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 95 - 260 8.4e-28 NB-ARC domain
LRR_8 PF13855 467 - 525 1.5e-09 Leucine rich repeat
LRR_14 PF23598 520 - 606 4.5e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 778 - 910 4.8e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 850 - 975 1.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2151
AccB7I CCANNNNNTGG 1 cut(s) 1390
AccI GTMKAC 2 cut(s) 348, 853
AccII CGCG 1 cut(s) 1143
AciI CCGC 8 cut(s) 147, 330, 1141, 1160, 1276, 1501, 1523, 2366
AclWI GGATC 6 cut(s) 568, 581, 1718, 2380, 2541, 2574
AcoI YGGCCR 1 cut(s) 1313
AcsI RAATTY 8 cut(s) 140, 269, 471, 627, 1368, 1509, 1661, 1911
AcuI CTGAAG 3 cut(s) 93, 1287, 2360
AcvI CACGTG 1 cut(s) 2609
AfaI GTAC 5 cut(s) 788, 1808, 2077, 2837, 3071
AfiI CCNNNNNNNGG 4 cut(s) 706, 1390, 1695, 2737
AflIII ACRYGT 2 cut(s) 235, 2273
AjnI CCWGG 4 cut(s) 2328, 2368, 2619, 2658
AjuI GAANNNNNNNTTGG 4 cut(s) 1857, 1889, 2493, 2525
AloI GAACNNNNNNTCC 4 cut(s) 959, 991, 2202, 2234
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 1 cut(s) 945
AlwI GGATC 6 cut(s) 568, 581, 1718, 2380, 2541, 2574
AoxI GGCC 6 cut(s) 882, 1167, 1270, 1313, 2759, 3022
ApaI GGGCCC 1 cut(s) 886
ApeKI GCWGC 6 cut(s) 95, 275, 920, 2001, 2004, 2898
ApoI RAATTY 8 cut(s) 140, 269, 471, 627, 1368, 1509, 1661, 1911
AspA2I CCTAGG 1 cut(s) 65
AspLEI GCGC 3 cut(s) 313, 848, 1145
AspS9I GGNCC 6 cut(s) 882, 883, 1168, 2401, 2597, 3023
AsuHPI GGTGA 5 cut(s) 133, 394, 905, 1297, 2369
AsuII TTCGAA 1 cut(s) 1022
AvaII GGWCC 2 cut(s) 2401, 2597
AvrII CCTAGG 1 cut(s) 65
BaeGI GKGCMC 1 cut(s) 886
BaeI ACNNNNGTAYC 2 cut(s) 2300, 2333
BalI TGGCCA 1 cut(s) 1315
BamHI GGATCC 1 cut(s) 573
BanII GRGCYC 1 cut(s) 886
BbrPI CACGTG 1 cut(s) 2609
BbsI GAAGAC 6 cut(s) 99, 524, 966, 1254, 2715, 2789
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 6 cut(s) 82, 287, 907, 2013, 2016, 2885
BccI CCATC 6 cut(s) 903, 959, 1774, 2446, 2548, 2926
BceAI ACGGC 2 cut(s) 367, 684
BciT130I CCWGG 4 cut(s) 2330, 2370, 2621, 2660
BciVI GTATCC 2 cut(s) 45, 457
BclI TGATCA 1 cut(s) 432
BcoDI GTCTC 1 cut(s) 945
BfaI CTAG 5 cut(s) 66, 837, 1182, 1200, 2468
BfmI CTRYAG 1 cut(s) 2380
BfuI GTATCC 2 cut(s) 45, 457
BglII AGATCT 2 cut(s) 81, 1492
BlnI CCTAGG 1 cut(s) 65
BmcAI AGTACT 1 cut(s) 2077
Bme1390I CCNGG 4 cut(s) 2330, 2370, 2621, 2660
Bme18I GGWCC 2 cut(s) 2401, 2597
BmgT120I GGNCC 6 cut(s) 882, 883, 1168, 2401, 2597, 3023
BmiI GGNNCC 5 cut(s) 575, 884, 2095, 2951, 2982
BmrFI CCNGG 4 cut(s) 2330, 2370, 2621, 2660
BmrI ACTGGG 2 cut(s) 579, 1843
BmsI GCATC 7 cut(s) 316, 644, 1202, 1212, 1315, 1753, 1924
BmuI ACTGGG 2 cut(s) 579, 1843
BoxI GACNNNNGTC 1 cut(s) 1575
BpiI GAAGAC 6 cut(s) 99, 524, 966, 1254, 2715, 2789
BpmI CTGGAG 1 cut(s) 1391
Bpu14I TTCGAA 1 cut(s) 1022
BpuEI CTTGAG 3 cut(s) 1646, 2083, 2905
BsaAI YACGTR 2 cut(s) 88, 2609
BsaJI CCNNGG 4 cut(s) 65, 705, 2194, 2659
Bsc4I CCNNNNNNNGG 4 cut(s) 706, 1390, 1695, 2737
Bse1I ACTGG 3 cut(s) 574, 1838, 2111
Bse3DI GCAATG 2 cut(s) 1254, 2167
BseBI CCWGG 4 cut(s) 2330, 2370, 2621, 2660
BseDI CCNNGG 4 cut(s) 65, 705, 2194, 2659
BseLI CCNNNNNNNGG 4 cut(s) 706, 1390, 1695, 2737
BseMI GCAATG 2 cut(s) 1254, 2167
BseMII CTCAG 5 cut(s) 66, 1721, 2127, 2537, 2702
BseNI ACTGG 3 cut(s) 574, 1838, 2111
BseSI GKGCMC 1 cut(s) 886
BseXI GCAGC 6 cut(s) 82, 287, 907, 2013, 2016, 2885
BseYI CCCAGC 2 cut(s) 496, 2120
Bsh1236I CGCG 1 cut(s) 1143
BshFI GGCC 6 cut(s) 884, 1169, 1272, 1315, 2761, 3024
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 2762
BslFI GGGAC 1 cut(s) 1844
BslI CCNNNNNNNGG 4 cut(s) 706, 1390, 1695, 2737
BsmAI GTCTC 1 cut(s) 945
BsmBI CGTCTC 1 cut(s) 945
BsmFI GGGAC 1 cut(s) 1844
BsmI GAATGC 5 cut(s) 196, 313, 848, 1944, 2269
BsnI GGCC 6 cut(s) 884, 1169, 1272, 1315, 2761, 3024
Bsp119I TTCGAA 1 cut(s) 1022
Bsp120I GGGCCC 1 cut(s) 882
Bsp1286I GDGCHC 2 cut(s) 400, 886
Bsp1407I TGTACA 2 cut(s) 1806, 2835
Bsp19I CCATGG 1 cut(s) 705
BspACI CCGC 8 cut(s) 147, 330, 1141, 1160, 1276, 1501, 1523, 2366
BspANI GGCC 6 cut(s) 884, 1169, 1272, 1315, 2761, 3024
BspCNI CTCAG 5 cut(s) 65, 1722, 2128, 2538, 2701
BspFNI CGCG 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 5 cut(s) 575, 884, 2095, 2951, 2982
BspMAI CTGCAG 1 cut(s) 2384
BspPI GGATC 6 cut(s) 568, 581, 1718, 2380, 2541, 2574
BspQI GCTCTTC 1 cut(s) 526
BspT104I TTCGAA 1 cut(s) 1022
BsrDI GCAATG 2 cut(s) 1254, 2167
BsrGI TGTACA 2 cut(s) 1806, 2835
BsrI ACTGG 3 cut(s) 574, 1838, 2111
BssECI CCNNGG 4 cut(s) 65, 705, 2194, 2659
BssT1I CCWWGG 2 cut(s) 65, 705
Bst2UI CCWGG 4 cut(s) 2330, 2370, 2621, 2660
Bst4CI ACNGT 7 cut(s) 1683, 1806, 2195, 2313, 2770, 2788, 2814
Bst6I CTCTTC 3 cut(s) 526, 596, 2675
BstAUI TGTACA 2 cut(s) 1806, 2835
BstBAI YACGTR 2 cut(s) 88, 2609
BstBI TTCGAA 1 cut(s) 1022
BstC8I GCNNGC 8 cut(s) 6, 49, 1141, 1187, 1274, 1294, 1376, 1742
BstDSI CCRYGG 2 cut(s) 705, 2194
BstFNI CGCG 1 cut(s) 1143
BstHHI GCGC 3 cut(s) 313, 848, 1145
BstMAI GTCTC 1 cut(s) 945
BstMWI GCNNNNNNNGC 4 cut(s) 26, 360, 869, 1166
BstNI CCWGG 4 cut(s) 2330, 2370, 2621, 2660
BstNSI RCATGY 6 cut(s) 239, 1132, 1813, 1930, 2277, 2590
BstPAI GACNNNNGTC 1 cut(s) 1575
BstSCI CCNGG 4 cut(s) 2328, 2368, 2619, 2658
BstSFI CTRYAG 1 cut(s) 2380
BstSLI GKGCMC 1 cut(s) 886
BstUI CGCG 1 cut(s) 1143
BstV1I GCAGC 6 cut(s) 82, 287, 907, 2013, 2016, 2885
BstV2I GAAGAC 6 cut(s) 99, 524, 966, 1254, 2715, 2789
BstX2I RGATCY 5 cut(s) 81, 573, 1492, 2372, 2566
BstYI RGATCY 5 cut(s) 81, 573, 1492, 2372, 2566
BsuI GTATCC 2 cut(s) 45, 457
BsuRI GGCC 6 cut(s) 884, 1169, 1272, 1315, 2761, 3024
BtgI CCRYGG 2 cut(s) 705, 2194
BtsIMutI CAGTG 6 cut(s) 1316, 1503, 1717, 1727, 2696, 2775
Cac8I GCNNGC 8 cut(s) 6, 49, 1141, 1187, 1274, 1294, 1376, 1742
CciI TCATGA 1 cut(s) 303
CfoI GCGC 3 cut(s) 313, 848, 1145
Cfr13I GGNCC 6 cut(s) 882, 883, 1168, 2401, 2597, 3023
CseI GACGC 2 cut(s) 927, 1205
Csp6I GTAC 5 cut(s) 787, 1807, 2076, 2836, 3070
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviQI GTAC 5 cut(s) 787, 1807, 2076, 2836, 3070
DraI TTTAAA 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 1313
Eam1104I CTCTTC 3 cut(s) 526, 596, 2675
EarI CTCTTC 3 cut(s) 526, 596, 2675
Eco130I CCWWGG 2 cut(s) 65, 705
Eco24I GRGCYC 1 cut(s) 886
Eco32I GATATC 3 cut(s) 1069, 1447, 1779
Eco47I GGWCC 2 cut(s) 2401, 2597
Eco57I CTGAAG 3 cut(s) 93, 1287, 2360
Eco72I CACGTG 1 cut(s) 2609
EcoO109I RGGNCCY 3 cut(s) 882, 883, 1168
EcoRI GAATTC 2 cut(s) 269, 627
EcoRII CCWGG 4 cut(s) 2328, 2368, 2619, 2658
EcoRV GATATC 3 cut(s) 1069, 1447, 1779
EcoT14I CCWWGG 2 cut(s) 65, 705
EcoT22I ATGCAT 3 cut(s) 986, 1217, 1227
EcoT38I GRGCYC 1 cut(s) 886
ErhI CCWWGG 2 cut(s) 65, 705
Esp3I CGTCTC 1 cut(s) 945
FaqI GGGAC 1 cut(s) 1844
FauI CCCGC 1 cut(s) 1148
FbaI TGATCA 1 cut(s) 432
FblI GTMKAC 2 cut(s) 348, 853
FriOI GRGCYC 1 cut(s) 886
FspBI CTAG 5 cut(s) 66, 837, 1182, 1200, 2468
GlaI GCGC 3 cut(s) 312, 847, 1144
GsaI CCCAGC 2 cut(s) 500, 2124
GsuI CTGGAG 1 cut(s) 1391
HaeIII GGCC 6 cut(s) 884, 1169, 1272, 1315, 2761, 3024
HapII CCGG 1 cut(s) 2762
HgaI GACGC 2 cut(s) 927, 1205
HhaI GCGC 3 cut(s) 313, 848, 1145
Hin6I GCGC 3 cut(s) 311, 846, 1143
HinP1I GCGC 3 cut(s) 311, 846, 1143
HincII GTYRAC 3 cut(s) 1207, 1686, 2116
HindII GTYRAC 3 cut(s) 1207, 1686, 2116
HindIII AAGCTT 2 cut(s) 1554, 1622
HinfI GANTC 9 cut(s) 203, 345, 752, 1121, 1178, 1485, 1630, 2065, 2887
HpaII CCGG 1 cut(s) 2762
HphI GGTGA 5 cut(s) 133, 394, 905, 1297, 2369
Hpy166II GTNNAC 7 cut(s) 349, 854, 1207, 1686, 1981, 2116, 2773
Hpy188III TCNNGA 9 cut(s) 163, 266, 304, 1118, 1238, 1408, 1853, 2282, 2468
Hpy8I GTNNAC 7 cut(s) 349, 854, 1207, 1686, 1981, 2116, 2773
Hpy99I CGWCG 2 cut(s) 930, 3035
HpyCH4III ACNGT 7 cut(s) 1683, 1806, 2195, 2313, 2770, 2788, 2814
HpyCH4IV ACGT 3 cut(s) 87, 1750, 2608
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 360, 869, 1166
HpySE526I ACGT 3 cut(s) 87, 1750, 2608
HspAI GCGC 3 cut(s) 311, 846, 1143
Ksp22I TGATCA 1 cut(s) 432
LguI GCTCTTC 1 cut(s) 526
LmnI GCTCC 3 cut(s) 395, 759, 2608
Lsp1109I GCAGC 6 cut(s) 82, 287, 907, 2013, 2016, 2885
LweI GCATC 7 cut(s) 316, 644, 1202, 1212, 1315, 1753, 1924
MaeI CTAG 5 cut(s) 66, 837, 1182, 1200, 2468
MaeII ACGT 3 cut(s) 87, 1750, 2608
MaeIII GTNAC 7 cut(s) 121, 2171, 2202, 2361, 2414, 2719, 3055
MfeI CAATTG 2 cut(s) 1074, 2702
MflI RGATCY 5 cut(s) 81, 573, 1492, 2372, 2566
MhlI GDGCHC 2 cut(s) 400, 886
MlsI TGGCCA 1 cut(s) 1315
MluNI TGGCCA 1 cut(s) 1315
MlyI GAGTC 4 cut(s) 354, 1494, 2074, 2896
MmeI TCCRAC 6 cut(s) 346, 373, 1149, 1683, 2511, 2544
Mox20I TGGCCA 1 cut(s) 1315
Mph1103I ATGCAT 3 cut(s) 986, 1217, 1227
MscI TGGCCA 1 cut(s) 1315
MslI CAYNNNNRTG 2 cut(s) 1220, 2180
Msp20I TGGCCA 1 cut(s) 1315
MspA1I CMGCKG 2 cut(s) 147, 1276
MspI CCGG 1 cut(s) 2762
MspR9I CCNGG 4 cut(s) 2330, 2370, 2621, 2660
MunI CAATTG 2 cut(s) 1074, 2702
Mva1269I GAATGC 5 cut(s) 196, 313, 848, 1944, 2269
MvaI CCWGG 4 cut(s) 2330, 2370, 2621, 2660
MvnI CGCG 1 cut(s) 1143
MwoI GCNNNNNNNGC 4 cut(s) 26, 360, 869, 1166
NcoI CCATGG 1 cut(s) 705
NlaIV GGNNCC 5 cut(s) 575, 884, 2095, 2951, 2982
NmuCI GTSAC 1 cut(s) 121
NsiI ATGCAT 3 cut(s) 986, 1217, 1227
NspI RCATGY 6 cut(s) 239, 1132, 1813, 1930, 2277, 2590
NspV TTCGAA 1 cut(s) 1022
PagI TCATGA 1 cut(s) 303
PciI ACATGT 2 cut(s) 235, 2273
PciSI GCTCTTC 1 cut(s) 526
PctI GAATGC 5 cut(s) 196, 313, 848, 1944, 2269
PfeI GAWTC 5 cut(s) 203, 752, 1121, 1178, 1630
PflMI CCANNNNNTGG 1 cut(s) 1390
PleI GAGTC 4 cut(s) 353, 1493, 2073, 2895
PmaCI CACGTG 1 cut(s) 2609
PmlI CACGTG 1 cut(s) 2609
PpsI GAGTC 4 cut(s) 353, 1493, 2073, 2895
Ppu21I YACGTR 2 cut(s) 88, 2609
PscI ACATGT 2 cut(s) 235, 2273
PshAI GACNNNNGTC 1 cut(s) 1575
PsiI TTATAA 1 cut(s) 2151
Psp6I CCWGG 4 cut(s) 2328, 2368, 2619, 2658
PspCI CACGTG 1 cut(s) 2609
PspFI CCCAGC 2 cut(s) 496, 2120
PspGI CCWGG 4 cut(s) 2328, 2368, 2619, 2658
PspN4I GGNNCC 5 cut(s) 575, 884, 2095, 2951, 2982
PspOMI GGGCCC 1 cut(s) 882
PspPI GGNCC 6 cut(s) 882, 883, 1168, 2401, 2597, 3023
PstI CTGCAG 1 cut(s) 2384
PsuI RGATCY 5 cut(s) 81, 573, 1492, 2372, 2566
RsaI GTAC 5 cut(s) 788, 1808, 2077, 2837, 3071
RsaNI GTAC 5 cut(s) 787, 1807, 2076, 2836, 3070
RseI CAYNNNNRTG 2 cut(s) 1220, 2180
SapI GCTCTTC 1 cut(s) 526
Sau96I GGNCC 6 cut(s) 882, 883, 1168, 2401, 2597, 3023
ScaI AGTACT 1 cut(s) 2077
SchI GAGTC 4 cut(s) 354, 1494, 2074, 2896
ScrFI CCNGG 4 cut(s) 2330, 2370, 2621, 2660
SduI GDGCHC 2 cut(s) 400, 886
SfaNI GCATC 7 cut(s) 316, 644, 1202, 1212, 1315, 1753, 1924
SfcI CTRYAG 1 cut(s) 2380
SfuI TTCGAA 1 cut(s) 1022
SinI GGWCC 2 cut(s) 2401, 2597
SmiMI CAYNNNNRTG 2 cut(s) 1220, 2180
SmlI CTYRAG 3 cut(s) 1625, 2062, 2884
SmoI CTYRAG 3 cut(s) 1625, 2062, 2884
SsiI CCGC 8 cut(s) 147, 330, 1141, 1160, 1276, 1501, 1523, 2366
SspI AATATT 2 cut(s) 1817, 1873
SspMI CTAG 5 cut(s) 66, 837, 1182, 1200, 2468
StyD4I CCNGG 4 cut(s) 2328, 2368, 2619, 2658
StyI CCWWGG 2 cut(s) 65, 705
TaaI ACNGT 7 cut(s) 1683, 1806, 2195, 2313, 2770, 2788, 2814
TaiI ACGT 3 cut(s) 90, 1753, 2611
TatI WGTACW 3 cut(s) 1806, 2075, 2835
TauI GCSGC 4 cut(s) 150, 332, 1503, 1525
TfiI GAWTC 5 cut(s) 203, 752, 1121, 1178, 1630
TscAI CASTG 6 cut(s) 1323, 1510, 1717, 1734, 2696, 2775
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 6 cut(s) 95, 275, 920, 2001, 2004, 2898
Tsp45I GTSAC 1 cut(s) 121
TspGWI ACGGA 2 cut(s) 1224, 2393
TspRI CASTG 6 cut(s) 1323, 1510, 1717, 1734, 2696, 2775
Van91I CCANNNNNTGG 1 cut(s) 1390
VpaK11BI GGWCC 2 cut(s) 2401, 2597
XapI RAATTY 8 cut(s) 140, 269, 471, 627, 1368, 1509, 1661, 1911
XbaI TCTAGA 1 cut(s) 2467
XceI RCATGY 6 cut(s) 239, 1132, 1813, 1930, 2277, 2590
XmaJI CCTAGG 1 cut(s) 65
XmiI GTMKAC 2 cut(s) 348, 853
XspI CTAG 5 cut(s) 66, 837, 1182, 1200, 2468
ZrmI AGTACT 1 cut(s) 2077
Zsp2I ATGCAT 3 cut(s) 986, 1217, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.