Rmu_sc0000908.1_g000021

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000908.1
OG0000085
Physical Location & Seq
Reverse (-)
86546 .. 98135
11590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000908.1_g000021.1.cds

Sequence Viewer

Length: 4851 bp
atgaagatgattattgtgattagtgcggtaatggtgcaatggttcggtgtcatagcctgtcttaagtggatatggaagaattggttccttattgtggtgattgtgacgtggaacgtaatagccagtcttgggtggatatggaagagtaggttcagttacatggttgaaaccccagagccaatatcagaggataatcaagaaccagttgacgtcgatgtctctgcatctcttccttcatcatcagctgaattaacagctcctacgtggaagcatgatgtgtttttgagcttcaggggtgtagacactcgtaagaatattacattctttttatacaatcggctgcaagaaagggaaatcaaaacattcatggatgaacgagacattgaagtaggggatgttatttctcccactctcctaacggcaattgaagaatcaaggttggcgattattgttctctctcaaaactatgcctcttcctcttggtgtttggaggaactaggacacatttgtaaaagcatgaaaggagacgagtcagacgacaatagaattctgccacttttttataatgtgcatcctagtgatgtacgacatcagacgagcaggttcggagatgctttcactaagcttaagaaatctgggcgacacgaatcagcgaaggtggagcagtggatagatactttaacaaaagttgcacaaatctccgggtggcattcactggaatttaaaaatgacggagaacttttgaataacattgtggaagctgtgtgcaagaaattactaccgactgaatctgtgttaaaaatacccctcggagattttgaagaatttgcagcaacaaaagaagccattcatgaggtcatgaaggcgctaaaagatggtgaggccactaccattggagtctacggcatggggggcgttggaaagacaacaatggtgaaacatgtcggtgtacaagcccaagaagaaaagctttttaataaagtgattatggctgtcgtatcccaaaaacccgacttgaaggaaattcaacaggaatttgcagaaacgctgggctttgaactcaaggagaagacggaaatggtaagagcccgtaaattgaagatgaaaataatgagcgaaaccaggatcctcataatcttggatgacatttggaagagaatagacttttcaagaattggaattccgagcgacaacgaacttcaaaaatgcaattccaaagtcctattgaccaccagaagtttgaaactttgtaggatgatggggacccatgcaaaaattcccctcaatatcttatcagaagaagattcttggcgcttatttgtgagggaagcggggatgtcttctgacaaatcttccaatttctctgatgtagcgagtaaggtggcaagagaatgcgccggtctaccagttgctttgatagcagttgcgagggcccttcgagatgaagatttggacagatgggaagaagctgcacgacaactaaaagcgtctcaacctcccatccgtgaagatgagggagatgtgtttgcatgcataaagttgagctatgatcacttgaaatctgatgattccaaatcatgcttcttgctttgctgcttgttcccagaagattataatatcccaattgaatacttggtcatgtatggaattgggaaaggaatgtttcaagatttaaccatgcaacgagcccgagactcaacatattcagtggtgcaggaccttaaagattctagcttgcttttaggcggtagagatgacggatgtgtaaggatgcatgatgtcattcgggatacggcaatacgaatttcctcttctgaagatggccagcagtccttggtgaaagttggctgtgaattgacgaattggccaaagattgatgcacataaaggctactctgcaatctcactaatgaagaacaaaatttgcaagctaccaaaaaagttggtatgtccaaatctccagattttattactacgacagaatgcttctttatgtcatatcccagaatcttttttccaaagtcagaatgaattaagagtcttggatcttagcgacactcgtatttcattactacaatcaatcggtttcctaaccaaccttcaagctttgtatttagattattgcagaaacataattgacacttctgtaatcggaaaacttcacaagcttgagattcttagtatgagaggaactgctctgaaagaattgtcaagagaaatagaacaactgaccaatctaaggatgctcgatataagtgctgcagctttatccttgagaggaagtatttgcaaaattccatctaaagtgatatcaaagttgcataaattagaagagctgtacatgcaatatgcattttgggactgggggaggaaaattgatggaaaaggagaagaaactaatattggctttgatgaattagctggtttatcatatttaagaattttgaaagtttgcatattagatgcaaattgcatccctaaaagtgttgaggtcgaaccgaattgggattactttgatattagtatttgtagcgccaagagtggacagggaatctataacatccttcgatctggtaataattatagatccttgagtcttgatagtactgacacaaccatgggaacctttccggactggtttgtcaacgctgtggtaaagaatactgagaggctgcgttatgaagaatgcaaatgcttaagtaacattcttgtggaatatgaccgtgggaagttacatggactgaaagttctctctgtaaaaggtcgctgtgagaacttgaaagaattgatgaatgcaataacatgtgttccagatccagatatgcctgtgtttgagaagttggaagagttgtatctggaatacctggatgacctgaagcagttatgtgttggtgagttaccacctcgatctctctgcaatctgaagttattgaaagtcgcttgctgtcataacttggggaatttgctgttgccatcaaaattgttgcagaaactaccaaatttggaaatactaaactgtgaggaaagtaaagtggatcatgtgtttgaatgtaaaggatttgagccagatcaaacaaatctgagagagatggagttgcgagatctagacgtaataagaagcatatgtaatggtcctgctccacgcagaatgttccaggctctaaagcaattgggattttatcattctccaacttccggcgaagcccccgcgccacagaagctgaagaaagaacctaccgatgacgaaaagagaaggatgaaaatagtggccggaagcttgatgaaagcagtgatcagacctggtggaggtgattcaacacctgaagatggtcatcaggccatatatcattgcactgttagaacattggatggagttattgttgaatcttcaagatcagaatttggtgcagccagtgaattgtacacagagctgatctacaaaacccacatggccatactacggggagctagccaacagctgctgacgggttccgcctttcttgactctgaattttgttctaggtataattcctttggatttaagcacggaatcatcgctgcatcatattcctttgtgggtgttcgtggtatctccgatataatcacgattccggggctggcaaatgtcgactttgctgatgtaagggctataatggtgaatgcgggttcttcattgatgttgataggaactgcaactgggaagacaagggcaagagatgctgctttaaatgccattcaatcatctttattagatattgttatagagagggctactggaattgtctggaacataactggtggaactgatttgacacactatgaggtaaatgctgcagcagaggttatatatgatcttgttgatccaacagcaaatttaatatttggagcagtgacagatccatcactcagtggtcaagttagcatcactctaattgctactggattcaaacgccaagaagaaagtgatgggaggccactccaggcacaaggagatattacccttggaatcaatcaaagagcttcctccttgtcacaaggtagttcagtcgagattcccgatttcttgaagaagaaaaggatgctcacgtcacaggttactggaggcctagcttgtggaactcctcatatgatcagtgctacgattgaaggccttgcttgttggcgtaagtttgatctgagtttggatcaggctcaagctagtagtggtatacttcctctgtgggtgagagctgtggccttggctgtctcgttggtgagaagtgatggtgatggtgatgctgaggatgaggatgagctcatttctttaaagccacggaggagggcaatggttctcaatttgggtgcaattgctggtagtatgaatggcagcggtttggctgaccagatcgacctcatcaaaatacgatttcagtccacagcaaaatcagactcaaatccgaagaagcgcaaacccgaaatctgcagccggcatcgaatcaagccgatcgcagccccaagaacgagaccagcaactattgcccccaaccaccgtgaccaccatcccctaacacctccgcctttcttccacttgttacagatcgagacgtcccaccagtctacacagaccaccgtcgtcgaggtcaccatcctcagaaccacagccctaccactccaaacaaaccatacaacccctgggacaccccaccagaacctggccagagatcgatctgacctcagatcaccaagaaactccaagccaaaggcagagcacccagacaagcccgatccgaacacacaaacaccggatcggggagaacaaccccatccacacacatctccaagcccgatctggaccctccctcgccgacaatga

Protein Analysis

1616

Amino Acids

180.46

Weight (kDa)

6.51

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 1614
AasI GACNNNNNNGTC 2 cut(s) 215, 2639
AatII GACGTC 2 cut(s) 213, 4586
Acc36I ACCTGC 1 cut(s) 591
AccB7I CCANNNNNTGG 1 cut(s) 4690
AccI GTMKAC 6 cut(s) 300, 900, 1402, 3627, 4207, 4595
AccII CGCG 1 cut(s) 3209
AccIII TCCGGA 1 cut(s) 2629
AciI CCGC 8 cut(s) 26, 1331, 1746, 3207, 3492, 3662, 4368, 4553
AcoI YGGCCR 5 cut(s) 1822, 1865, 3267, 3450, 4692
AcuI CTGAAG 6 cut(s) 274, 1836, 2894, 2942, 3242, 3342
AcyI GRCGYC 2 cut(s) 210, 4583
AdeI CACNNNGTG 1 cut(s) 3908
AfaI GTAC 5 cut(s) 585, 951, 2339, 2605, 3422
AfiI CCNNNNNNNGG 9 cut(s) 94, 129, 2238, 3194, 3305, 3326, 3459, 4690, 4787
AflII CTTAAG 3 cut(s) 62, 626, 2695
AflIII ACRYGT 2 cut(s) 940, 2801
AjiI CACGTC 2 cut(s) 108, 4086
AjnI CCWGG 7 cut(s) 1121, 2862, 3153, 3298, 3978, 4669, 4689
AjuI GAANNNNNNNTTGG 4 cut(s) 1565, 1597, 2385, 2417
AloI GAACNNNNNNTCC 2 cut(s) 2730, 2762
Alw21I GWGCWC 2 cut(s) 4296, 4749
Alw26I GTCTC 8 cut(s) 223, 372, 519, 1494, 1686, 4249, 4495, 4574
AlwNI CAGNNNCTG 3 cut(s) 3220, 3481, 4690
Ama87I CYCGRG 1 cut(s) 1689
Aor13HI TCCGGA 1 cut(s) 2629
ApaI GGGCCC 1 cut(s) 1435
Asp700I GAANNNNTTC 2 cut(s) 83, 846
AspLEI GCGC 6 cut(s) 868, 1314, 1397, 2534, 3211, 4446
AspS9I GGNCC 6 cut(s) 1263, 1431, 1432, 1717, 3131, 4830
AsuC2I CCSGG 2 cut(s) 703, 3612
AsuNHI GCTAGC 1 cut(s) 3467
AvaI CYCGRG 1 cut(s) 1689
AvaII GGWCC 4 cut(s) 1263, 1717, 3131, 4830
BaeGI GKGCMC 1 cut(s) 1435
BalI TGGCCA 4 cut(s) 1824, 1867, 3452, 4694
BamHI GGATCC 1 cut(s) 1125
BanII GRGCYC 4 cut(s) 1090, 1435, 1690, 4296
BbsI GAAGAC 3 cut(s) 1076, 1332, 3707
Bbv12I GWGCWC 2 cut(s) 4296, 4749
BbvCI CCTCAGC 1 cut(s) 4278
BceAI ACGGC 3 cut(s) 435, 919, 1809
BcgI CGANNNNNNTGC 6 cut(s) 203, 237, 2237, 2271, 2895, 2929
BciT130I CCWGG 7 cut(s) 1123, 2864, 3155, 3300, 3980, 4671, 4691
BciVI GTATCC 2 cut(s) 1009, 1783
BclI TGATCA 3 cut(s) 1549, 3291, 4128
BcnI CCSGG 2 cut(s) 703, 3612
BcoDI GTCTC 8 cut(s) 223, 372, 519, 1494, 1686, 4249, 4495, 4574
BfaI CTAG 8 cut(s) 497, 576, 1731, 3104, 3468, 3519, 4106, 4197
BfmI CTRYAG 3 cut(s) 2259, 3831, 4459
BfoI RGCGCY 3 cut(s) 869, 1315, 2535
BfrI CTTAAG 3 cut(s) 62, 626, 2695
BfuAI ACCTGC 1 cut(s) 591
BfuI GTATCC 2 cut(s) 1009, 1783
BglII AGATCT 1 cut(s) 3100
BmcAI AGTACT 1 cut(s) 2605
Bme1390I CCNGG 9 cut(s) 703, 1123, 2864, 3155, 3300, 3612, 3980, 4671, 4691
Bme18I GGWCC 4 cut(s) 1263, 1717, 3131, 4830
BmeT110I CYCGRG 1 cut(s) 1689
BmgBI CACGTC 2 cut(s) 108, 4086
BmgT120I GGNCC 6 cut(s) 1263, 1431, 1432, 1717, 3131, 4830
BmiI GGNNCC 8 cut(s) 86, 1127, 1264, 1265, 1433, 2623, 3490, 4832
BmrFI CCNGG 9 cut(s) 703, 1123, 2864, 3155, 3300, 3612, 3980, 4671, 4691
BmrI ACTGGG 2 cut(s) 2371, 3705
BmtI GCTAGC 1 cut(s) 3471
BmuI ACTGGG 2 cut(s) 2371, 3705
BoxI GACNNNNGTC 1 cut(s) 4607
BpiI GAAGAC 3 cut(s) 1076, 1332, 3707
BpmI CTGGAG 3 cut(s) 1943, 3962, 4119
Bpu10I CCTNAGC 1 cut(s) 4278
BpuEI CTTGAG 5 cut(s) 1046, 2189, 2293, 2611, 4176
BpuMI CCSGG 2 cut(s) 703, 3612
Bsa29I ATCGAT 1 cut(s) 4702
BsaAI YACGTR 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 3330
BsaHI GRCGYC 2 cut(s) 210, 4583
BsaI GGTCTC 1 cut(s) 4495
BsaWI WCCGGW 2 cut(s) 2629, 4780
BsaXI ACNNNNNCTCC 2 cut(s) 3363, 3393
Bsc4I CCNNNNNNNGG 9 cut(s) 94, 129, 2238, 3194, 3305, 3326, 3459, 4690, 4787
Bse118I RCCGGY 2 cut(s) 1397, 4464
Bse3DI GCAATG 3 cut(s) 44, 3346, 4329
Bse8I GATNNNNATC 1 cut(s) 3330
BseAI TCCGGA 1 cut(s) 2629
BseBI CCWGG 7 cut(s) 1123, 2864, 3155, 3300, 3980, 4671, 4691
BseCI ATCGAT 1 cut(s) 4702
BseJI GATNNNNATC 1 cut(s) 3330
BseLI CCNNNNNNNGG 9 cut(s) 94, 129, 2238, 3194, 3305, 3326, 3459, 4690, 4787
BseMI GCAATG 3 cut(s) 44, 3346, 4329
BseMII CTCAG 7 cut(s) 2655, 3071, 3919, 4166, 4269, 4642, 4726
BseRI GAGGAG 2 cut(s) 4110, 4330
BseSI GKGCMC 1 cut(s) 1435
BseYI CCCAGC 1 cut(s) 1048
BsgI GTGCAG 3 cut(s) 1455, 1733, 3426
Bsh1236I CGCG 1 cut(s) 3209
Bsh1285I CGRYCG 1 cut(s) 4485
BshVI ATCGAT 1 cut(s) 4702
BsiEI CGRYCG 1 cut(s) 4485
BsiHKAI GWGCWC 2 cut(s) 4296, 4749
BsiHKCI CYCGRG 1 cut(s) 1689
BsiSI CCGG 8 cut(s) 702, 1398, 2630, 3195, 3270, 3611, 4465, 4781
BslFI GGGAC 4 cut(s) 1276, 2372, 4570, 4687
BslI CCNNNNNNNGG 9 cut(s) 94, 129, 2238, 3194, 3305, 3326, 3459, 4690, 4787
BsmAI GTCTC 8 cut(s) 223, 372, 519, 1494, 1686, 4249, 4495, 4574
BsmBI CGTCTC 3 cut(s) 519, 1494, 4574
BsmFI GGGAC 4 cut(s) 1276, 2372, 4570, 4687
BsmI GAATGC 6 cut(s) 709, 1397, 1987, 2690, 2797, 3664
Bso31I GGTCTC 1 cut(s) 4495
BsoBI CYCGRG 1 cut(s) 1689
Bsp120I GGGCCC 1 cut(s) 1431
Bsp1286I GDGCHC 5 cut(s) 1090, 1435, 1690, 4296, 4749
Bsp13I TCCGGA 1 cut(s) 2629
Bsp1407I TGTACA 3 cut(s) 949, 2337, 3420
Bsp19I CCATGG 1 cut(s) 2616
BspACI CCGC 8 cut(s) 26, 1331, 1746, 3207, 3492, 3662, 4368, 4553
BspCNI CTCAG 7 cut(s) 2656, 3072, 3918, 4167, 4270, 4641, 4725
BspDI ATCGAT 1 cut(s) 4702
BspEI TCCGGA 1 cut(s) 2629
BspFNI CGCG 1 cut(s) 3209
BspHI TCATGA 2 cut(s) 850, 858
BspLI GGNNCC 8 cut(s) 86, 1127, 1264, 1265, 1433, 2623, 3490, 4832
BspMAI CTGCAG 3 cut(s) 2263, 3835, 4463
BspMI ACCTGC 1 cut(s) 591
BspOI GCTAGC 1 cut(s) 3471
BspQI GCTCTTC 1 cut(s) 2325
BspTI CTTAAG 3 cut(s) 62, 626, 2695
BspTNI GGTCTC 1 cut(s) 4495
BsrDI GCAATG 3 cut(s) 44, 3346, 4329
BsrFI RCCGGY 2 cut(s) 1397, 4464
BsrGI TGTACA 3 cut(s) 949, 2337, 3420
BssAI RCCGGY 2 cut(s) 1397, 4464
BssNAI GTATAC 1 cut(s) 4208
BssNI GRCGYC 2 cut(s) 210, 4583
BssT1I CCWWGG 4 cut(s) 1833, 2616, 4000, 4236
Bst1107I GTATAC 1 cut(s) 4208
Bst2UI CCWGG 7 cut(s) 1123, 2864, 3155, 3300, 3980, 4671, 4691
Bst4CI ACNGT 5 cut(s) 2723, 3017, 3355, 4529, 4609
Bst6I CTCTTC 7 cut(s) 137, 234, 478, 1148, 1816, 2325, 2838
BstACI GRCGYC 2 cut(s) 210, 4583
BstAFI CTTAAG 3 cut(s) 62, 626, 2695
BstAPI GCANNNNNTGC 2 cut(s) 3716, 4514
BstAUI TGTACA 3 cut(s) 949, 2337, 3420
BstBAI YACGTR 1 cut(s) 264
BstC8I GCNNGC 8 cut(s) 1531, 1736, 1826, 1928, 2941, 3469, 3618, 4466
BstDSI CCRYGG 3 cut(s) 2616, 2722, 4310
BstEII GGTNACC 1 cut(s) 4618
BstENI CCTNNNNNAGG 1 cut(s) 3303
BstFNI CGCG 1 cut(s) 3209
BstH2I RGCGCY 3 cut(s) 869, 1315, 2535
BstHHI GCGC 6 cut(s) 868, 1314, 1397, 2534, 3211, 4446
BstMAI GTCTC 8 cut(s) 223, 372, 519, 1494, 1686, 4249, 4495, 4574
BstMCI CGRYCG 1 cut(s) 4485
BstMWI GCNNNNNNNGC 7 cut(s) 912, 1418, 2341, 3217, 3716, 3728, 4514
BstNI CCWGG 7 cut(s) 1123, 2864, 3155, 3300, 3980, 4671, 4691
BstNSI RCATGY 4 cut(s) 944, 1533, 2344, 2805
BstPAI GACNNNNGTC 1 cut(s) 4607
BstPI GGTNACC 1 cut(s) 4618
BstSCI CCNGG 9 cut(s) 701, 1121, 2862, 3153, 3298, 3610, 3978, 4669, 4689
BstSFI CTRYAG 3 cut(s) 2259, 3831, 4459
BstSLI GKGCMC 1 cut(s) 1435
BstUI CGCG 1 cut(s) 3209
BstV2I GAAGAC 3 cut(s) 1076, 1332, 3707
BstX2I RGATCY 6 cut(s) 1125, 2044, 2585, 2812, 3100, 3895
BstYI RGATCY 6 cut(s) 1125, 2044, 2585, 2812, 3100, 3895
BstZ17I GTATAC 1 cut(s) 4208
Bsu15I ATCGAT 1 cut(s) 4702
BsuI GTATCC 2 cut(s) 1009, 1783
BsuTUI ATCGAT 1 cut(s) 4702
BtgI CCRYGG 3 cut(s) 2616, 2722, 4310
BtgZI GCGATG 1 cut(s) 3538
BtrI CACGTC 2 cut(s) 108, 4086
BtsI GCAGTG 3 cut(s) 671, 3294, 3894
BtsIMutI CAGTG 9 cut(s) 671, 713, 1713, 3294, 3351, 3418, 3894, 3913, 4138
BveI ACCTGC 1 cut(s) 591
Cac8I GCNNGC 8 cut(s) 1531, 1736, 1826, 1928, 2941, 3469, 3618, 4466
CaiI CAGNNNCTG 3 cut(s) 3220, 3481, 4690
CciI TCATGA 2 cut(s) 850, 858
CfoI GCGC 6 cut(s) 868, 1314, 1397, 2534, 3211, 4446
Cfr10I RCCGGY 2 cut(s) 1397, 4464
Cfr13I GGNCC 6 cut(s) 1263, 1431, 1432, 1717, 3131, 4830
ClaI ATCGAT 1 cut(s) 4702
CseI GACGC 1 cut(s) 1476
CsiI ACCWGGT 1 cut(s) 3298
Csp6I GTAC 5 cut(s) 584, 950, 2338, 2604, 3421
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviQI GTAC 5 cut(s) 584, 950, 2338, 2604, 3421
DraI TTTAAA 3 cut(s) 724, 3726, 4305
DraIII CACNNNGTG 1 cut(s) 3908
DrdI GACNNNNNNGTC 2 cut(s) 215, 2639
DseDI GACNNNNNNGTC 2 cut(s) 215, 2639
EaeI YGGCCR 5 cut(s) 1822, 1865, 3267, 3450, 4692
Eam1104I CTCTTC 7 cut(s) 137, 234, 478, 1148, 1816, 2325, 2838
EarI CTCTTC 7 cut(s) 137, 234, 478, 1148, 1816, 2325, 2838
EciI GGCGGA 2 cut(s) 3481, 4542
Ecl136II GAGCTC 1 cut(s) 4294
Eco130I CCWWGG 4 cut(s) 1833, 2616, 4000, 4236
Eco147I AGGCCT 2 cut(s) 4104, 4149
Eco24I GRGCYC 4 cut(s) 1090, 1435, 1690, 4296
Eco31I GGTCTC 1 cut(s) 4495
Eco32I GATATC 1 cut(s) 2310
Eco47I GGWCC 4 cut(s) 1263, 1717, 3131, 4830
Eco53kI GAGCTC 1 cut(s) 4294
Eco57I CTGAAG 6 cut(s) 274, 1836, 2894, 2942, 3242, 3342
Eco88I CYCGRG 1 cut(s) 1689
Eco91I GGTNACC 1 cut(s) 4618
EcoICRI GAGCTC 1 cut(s) 4294
EcoNI CCTNNNNNAGG 1 cut(s) 3303
EcoO109I RGGNCCY 4 cut(s) 1263, 1431, 1432, 1717
EcoO65I GGTNACC 1 cut(s) 4618
EcoRI GAATTC 2 cut(s) 546, 1179
EcoRII CCWGG 7 cut(s) 1121, 2862, 3153, 3298, 3978, 4669, 4689
EcoRV GATATC 1 cut(s) 2310
EcoT14I CCWWGG 4 cut(s) 1833, 2616, 4000, 4236
EcoT22I ATGCAT 3 cut(s) 1535, 1776, 2353
EcoT38I GRGCYC 4 cut(s) 1090, 1435, 1690, 4296
ErhI CCWWGG 4 cut(s) 1833, 2616, 4000, 4236
Esp3I CGTCTC 3 cut(s) 519, 1494, 4574
FalI AAGNNNNNCTT 2 cut(s) 954, 986
FaqI GGGAC 4 cut(s) 1276, 2372, 4570, 4687
FauI CCCGC 3 cut(s) 1324, 3214, 3655
FauNDI CATATG 2 cut(s) 3122, 4125
FbaI TGATCA 3 cut(s) 1549, 3291, 4128
FblI GTMKAC 6 cut(s) 300, 900, 1402, 3627, 4207, 4595
FriOI GRGCYC 4 cut(s) 1090, 1435, 1690, 4296
FspBI CTAG 8 cut(s) 497, 576, 1731, 3104, 3468, 3519, 4106, 4197
GlaI GCGC 6 cut(s) 867, 1313, 1396, 2533, 3210, 4445
GsaI CCCAGC 1 cut(s) 1052
GsuI CTGGAG 3 cut(s) 1943, 3962, 4119
HaeII RGCGCY 3 cut(s) 869, 1315, 2535
HapII CCGG 8 cut(s) 702, 1398, 2630, 3195, 3270, 3611, 4465, 4781
HgaI GACGC 1 cut(s) 1476
HhaI GCGC 6 cut(s) 868, 1314, 1397, 2534, 3211, 4446
Hin1I GRCGYC 2 cut(s) 210, 4583
Hin6I GCGC 6 cut(s) 866, 1312, 1395, 2532, 3209, 4444
HinP1I GCGC 6 cut(s) 866, 1312, 1395, 2532, 3209, 4444
HincII GTYRAC 3 cut(s) 208, 2644, 3628
HindII GTYRAC 3 cut(s) 208, 2644, 3628
HindIII AAGCTT 5 cut(s) 623, 968, 2103, 2165, 3274
HpaII CCGG 8 cut(s) 702, 1398, 2630, 3195, 3270, 3611, 4465, 4781
Hpy99I CGWCG 3 cut(s) 215, 4613, 4616
HpyAV CCTTC 9 cut(s) 243, 649, 856, 1012, 1445, 2108, 2573, 3246, 4139
HpyCH4III ACNGT 5 cut(s) 2723, 3017, 3355, 4529, 4609
HpyCH4IV ACGT 7 cut(s) 107, 114, 210, 263, 3108, 4085, 4583
HpyF10VI GCNNNNNNNGC 7 cut(s) 912, 1418, 2341, 3217, 3716, 3728, 4514
HpySE526I ACGT 7 cut(s) 107, 114, 210, 263, 3108, 4085, 4583
Hsp92I GRCGYC 2 cut(s) 210, 4583
HspAI GCGC 6 cut(s) 866, 1312, 1395, 2532, 3209, 4444
KflI GGGWCCC 1 cut(s) 1263
Kpn2I TCCGGA 1 cut(s) 2629
KroI GCCGGC 1 cut(s) 4464
KroNI GCCGGC 1 cut(s) 4466
Ksp22I TGATCA 3 cut(s) 1549, 3291, 4128
LguI GCTCTTC 1 cut(s) 2325
LmnI GCTCC 5 cut(s) 262, 661, 3142, 3464, 3884
MabI ACCWGGT 1 cut(s) 3298
MaeI CTAG 8 cut(s) 497, 576, 1731, 3104, 3468, 3519, 4106, 4197
MaeII ACGT 7 cut(s) 107, 114, 210, 263, 3108, 4085, 4583
MfeI CAATTG 4 cut(s) 423, 1623, 3167, 4344
MflI RGATCY 6 cut(s) 1125, 2044, 2585, 2812, 3100, 3895
MhlI GDGCHC 5 cut(s) 1090, 1435, 1690, 4296, 4749
MlsI TGGCCA 4 cut(s) 1824, 1867, 3452, 4694
MluNI TGGCCA 4 cut(s) 1824, 1867, 3452, 4694
MlyI GAGTC 7 cut(s) 539, 906, 1688, 2046, 2602, 3497, 4421
MmeI TCCRAC 4 cut(s) 898, 2820, 3212, 3887
Mox20I TGGCCA 4 cut(s) 1824, 1867, 3452, 4694
Mph1103I ATGCAT 3 cut(s) 1535, 1776, 2353
MroI TCCGGA 1 cut(s) 2629
MroNI GCCGGC 1 cut(s) 4464
MroXI GAANNNNTTC 2 cut(s) 83, 846
MscI TGGCCA 4 cut(s) 1824, 1867, 3452, 4694
MslI CAYNNNNRTG 4 cut(s) 576, 945, 2615, 2708
Msp20I TGGCCA 4 cut(s) 1824, 1867, 3452, 4694
MspA1I CMGCKG 3 cut(s) 245, 3478, 4368
MspCI CTTAAG 3 cut(s) 62, 626, 2695
MspI CCGG 8 cut(s) 702, 1398, 2630, 3195, 3270, 3611, 4465, 4781
MspR9I CCNGG 9 cut(s) 703, 1123, 2864, 3155, 3300, 3612, 3980, 4671, 4691
MunI CAATTG 4 cut(s) 423, 1623, 3167, 4344
Mva1269I GAATGC 6 cut(s) 709, 1397, 1987, 2690, 2797, 3664
MvaI CCWGG 7 cut(s) 1123, 2864, 3155, 3300, 3980, 4671, 4691
MvnI CGCG 1 cut(s) 3209
MwoI GCNNNNNNNGC 7 cut(s) 912, 1418, 2341, 3217, 3716, 3728, 4514
NaeI GCCGGC 1 cut(s) 4466
NciI CCSGG 2 cut(s) 703, 3612
NcoI CCATGG 1 cut(s) 2616
NdeI CATATG 2 cut(s) 3122, 4125
NgoMIV GCCGGC 1 cut(s) 4464
NheI GCTAGC 1 cut(s) 3467
NlaIV GGNNCC 8 cut(s) 86, 1127, 1264, 1265, 1433, 2623, 3490, 4832
NmuCI GTSAC 6 cut(s) 103, 3889, 4029, 4086, 4529, 4618
NsiI ATGCAT 3 cut(s) 1535, 1776, 2353
NspI RCATGY 4 cut(s) 944, 1533, 2344, 2805
PaeI GCATGC 1 cut(s) 1533
PagI TCATGA 2 cut(s) 850, 858
PasI CCCWGGG 1 cut(s) 4670
PceI AGGCCT 2 cut(s) 4104, 4149
PciI ACATGT 2 cut(s) 940, 2801
PciSI GCTCTTC 1 cut(s) 2325
PcsI WCGNNNNNNNCGW 2 cut(s) 534, 4053
PctI GAATGC 6 cut(s) 709, 1397, 1987, 2690, 2797, 3664
PdiI GCCGGC 1 cut(s) 4466
PdmI GAANNNNTTC 2 cut(s) 83, 846
PflMI CCANNNNNTGG 1 cut(s) 4690
Ple19I CGATCG 1 cut(s) 4485
PleI GAGTC 7 cut(s) 538, 905, 1688, 2045, 2601, 3497, 4421
PpsI GAGTC 7 cut(s) 538, 905, 1688, 2045, 2601, 3497, 4421
Ppu21I YACGTR 1 cut(s) 264
PpuMI RGGWCCY 2 cut(s) 1263, 1717
PscI ACATGT 2 cut(s) 940, 2801
PshAI GACNNNNGTC 1 cut(s) 4607
PsiI TTATAA 2 cut(s) 564, 1614
Psp124BI GAGCTC 1 cut(s) 4296
Psp5II RGGWCCY 2 cut(s) 1263, 1717
Psp6I CCWGG 7 cut(s) 1121, 2862, 3153, 3298, 3978, 4669, 4689
PspEI GGTNACC 1 cut(s) 4618
PspFI CCCAGC 1 cut(s) 1048
PspGI CCWGG 7 cut(s) 1121, 2862, 3153, 3298, 3978, 4669, 4689
PspN4I GGNNCC 8 cut(s) 86, 1127, 1264, 1265, 1433, 2623, 3490, 4832
PspOMI GGGCCC 1 cut(s) 1431
PspPI GGNCC 6 cut(s) 1263, 1431, 1432, 1717, 3131, 4830
PspPPI RGGWCCY 2 cut(s) 1263, 1717
PstI CTGCAG 3 cut(s) 2263, 3835, 4463
PstNI CAGNNNCTG 3 cut(s) 3220, 3481, 4690
PsuI RGATCY 6 cut(s) 1125, 2044, 2585, 2812, 3100, 3895
PvuI CGATCG 1 cut(s) 4485
PvuII CAGCTG 2 cut(s) 245, 3478
RsaI GTAC 5 cut(s) 585, 951, 2339, 2605, 3422
RsaNI GTAC 5 cut(s) 584, 950, 2338, 2604, 3421
RseI CAYNNNNRTG 4 cut(s) 576, 945, 2615, 2708
SacI GAGCTC 1 cut(s) 4296
SalI GTCGAC 1 cut(s) 3626
SapI GCTCTTC 1 cut(s) 2325
Sau96I GGNCC 6 cut(s) 1263, 1431, 1432, 1717, 3131, 4830
ScaI AGTACT 1 cut(s) 2605
SchI GAGTC 7 cut(s) 539, 906, 1688, 2046, 2602, 3497, 4421
ScrFI CCNGG 9 cut(s) 703, 1123, 2864, 3155, 3300, 3612, 3980, 4671, 4691
SduI GDGCHC 5 cut(s) 1090, 1435, 1690, 4296, 4749
SexAI ACCWGGT 1 cut(s) 3298
SfcI CTRYAG 3 cut(s) 2259, 3831, 4459
SinI GGWCC 4 cut(s) 1263, 1717, 3131, 4830
SmiMI CAYNNNNRTG 4 cut(s) 576, 945, 2615, 2708
SmlI CTYRAG 8 cut(s) 62, 626, 1061, 2168, 2272, 2590, 2695, 4191
SmoI CTYRAG 8 cut(s) 62, 626, 1061, 2168, 2272, 2590, 2695, 4191
SphI GCATGC 1 cut(s) 1533
SseBI AGGCCT 2 cut(s) 4104, 4149
SsiI CCGC 8 cut(s) 26, 1331, 1746, 3207, 3492, 3662, 4368, 4553
SspI AATATT 3 cut(s) 316, 2401, 3879
SspMI CTAG 8 cut(s) 497, 576, 1731, 3104, 3468, 3519, 4106, 4197
SstI GAGCTC 1 cut(s) 4296
StuI AGGCCT 2 cut(s) 4104, 4149
StyD4I CCNGG 9 cut(s) 701, 1121, 2862, 3153, 3298, 3610, 3978, 4669, 4689
StyI CCWWGG 4 cut(s) 1833, 2616, 4000, 4236
TaaI ACNGT 5 cut(s) 2723, 3017, 3355, 4529, 4609
TaiI ACGT 7 cut(s) 110, 117, 213, 266, 3111, 4088, 4586
TatI WGTACW 4 cut(s) 949, 2337, 2603, 3420
TscAI CASTG 9 cut(s) 671, 720, 1713, 3294, 3358, 3418, 3894, 3913, 4138
TseFI GTSAC 6 cut(s) 103, 3889, 4029, 4086, 4529, 4618
Tsp45I GTSAC 6 cut(s) 103, 3889, 4029, 4086, 4529, 4618
TspGWI ACGGA 6 cut(s) 747, 1088, 1493, 1773, 3561, 4327
TspRI CASTG 9 cut(s) 671, 720, 1713, 3294, 3358, 3418, 3894, 3913, 4138
Van91I CCANNNNNTGG 1 cut(s) 4690
Vha464I CTTAAG 3 cut(s) 62, 626, 2695
VpaK11BI GGWCC 4 cut(s) 1263, 1717, 3131, 4830
XagI CCTNNNNNAGG 1 cut(s) 3303
XbaI TCTAGA 1 cut(s) 3103
XceI RCATGY 4 cut(s) 944, 1533, 2344, 2805
XcmI CCANNNNNNNNNTGG 2 cut(s) 4667, 4824
XmiI GTMKAC 6 cut(s) 300, 900, 1402, 3627, 4207, 4595
XmnI GAANNNNTTC 2 cut(s) 83, 846
XspI CTAG 8 cut(s) 497, 576, 1731, 3104, 3468, 3519, 4106, 4197
ZraI GACGTC 2 cut(s) 211, 4584
ZrmI AGTACT 1 cut(s) 2605
Zsp2I ATGCAT 3 cut(s) 1535, 1776, 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.