Prupe.1G542800_v2.0.a1
WRKY Family

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000085
Physical Location & Seq
Forward (+)
44348548 .. 44353725
5178 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G542800.1

Sequence Viewer

Length: 3126 bp
ATGGCAGAAATTATTTTGGGAGTTATTGGTCCCGTAATGCAACTTGGTCAGTGGTTATGGAGTCCAGCAAAGCGTGGATTGGGTTACATGGTTCATTACAAGAGAAACGCAGAGTCTCTCAACCTTCAAATTGAAGAGCTTAAAGTTAAAAAAAAAGGTAATCAAAATTTAGTTGATGCGTTTCAACTAAATGGAGAAGAGCCTGAGATTAAAAAGTGGTTTGAAGATGCGAATAAGGCCATAGCAGATGCTGCACAATTAACTGGTGAAGTTGCAGCAAGTAAAAATTGCATTAGCGGGATGTGCCCGGATTTGAGATGGCGTTACAATTTAGGAAAGAAAGCAATGGAGGAGAAAGAAGCAGTAAACAAGCTACTAGAAAAGGGAGATTTTCAAACAATTTCTGTTCAAGTTCCACATCCCATTGAAATTGAGTCCACAATGTCGACTGGTGGTTTTCAAGCATTTGGATCAACAACAAAAGCCATGGATCAAGTCATGACAGCGCTGAAAGATGATAAGGTCACTGTGATTGGGGTCTATGGAATGGGAGGTGTCGGCAAAACAACCATGGTGAAACAGGTTGGTGCACAAGCCCGTAAAGATGGACTCTTTGATCATGTGATTATGGCTGTCTTTAGCCAAAACCCCGACATGATGAAGATTCAAGGCCAACTTTCAGATATGTTGGGCTTGAAATTGCAGGAGGAAACAGAATTGGGAAGAGCAGGTAGATTGAAGGAGAGGATACTAAGGGGAAACAAGACCCTTATAATCTTGGACGACATTTGGAATGCATCACAAACTTTGACTAGCATTGGAATTCCCAGCCCCATTGAACTGCAAGGATGCAATTCCAAAGTCCTGATAACCACAAGGAGATTGAATGTTTGTCACGGCATGGAGAGCAATGTAAAAGTACATCTCAATGTCCTATCTGAAGAAGATTCCTGGACCTTGTTTGCGAAAAGAGCAAGAAAGTCTTTTGAATCAGTCCCTTTCTATGAAGTGGCGAGAAAGGTAGCTCGTGAATGTGCTGGTCTTCCAATTGCATTGGTAGCAGTTGCAGGGGCACTTGGAGATAAAGACTTTGATGAGTGGAAAGAAGCAGCTCGACGACTGGAGATGTCCCAGCCTGCCAACCTTGAAGACGACGGAGATGTGTTCAAATGTATAAAGTTAAGCTATGAGTACTTGAAAGGCGAGGATGCCAAGTCATGCTTCTTGCTTTGCTCCCTATTCGCAGAAGACAGTCATATCGCCATACAAGACTTGTTTAGTTACGGGTTTGGGTATGGATTGTTTCGAGATGGCAATACATTGGAAGGAGCCAGAGCCAAAGCACGTACAGTGACCAAGTACCTTAAAGCTTCCAGCTTGCTTTTGGACGGGGAAAGCGAAAAATATTTGCGCATGCATGACGTCATACGAGATATGGCCATATTAATTGCATCATCAGAAGAACATGGCCATCGGTTTTTGGTAAAAGCCGGTTGGGAATTAAATGTCTGGCCAAATGATACAGATGAAGGCTGCTCTGCAATCTCGCTACAGGATAACTGTATAAGAAAGCTTCCTGATGAGTTGGTATGCCCAAAACTCCAAATTTTACTTCTAAATGATAATTCTACACTAGAGGAGATCCCAGAAGCTTTTTTCCAGAGTCTGAACGCATTAAGGGTCTTAGACCTTGCCGAAACTTCTATTTCAATACTACCCTCATCGTTCAATTTCCTAATAAACCTCCAAACATTGCATCTAGATGGATGTCCGGACTTAAAGGACATTTCCGTACTCGGAAAACTAAAGAAACTTGAGATTCTTAGTCTGAGATACTCTGGATTTAAAAAATTTCCAGAAGAAATAGGAAACTTGGCCAATCTGAGGTTGTTGGATTTGAGTTGGAATTCAGAGATAAACATAATTCCATCGAAAGTGATATCTAGGTTGTCTAGGTTAGAGGAACTACTCATGGAAGGTAGTTTTGGAGACTGGGGGGGTAAAGTTGAAGGGGCAGGGGAAAGAATTAATGCTGGCTTCGATGAGTTAACTTGCTTGTCGTACTTAAACATTTTGTTTGTTGGAATTTGTAATGTAGAACGCATTCCTAAAGACGTTGAGTTCCTTCCAAATTGGGAAAAATTCTTCATATGTATCAAGAGGGAATCACTCCCGATGGATTATTTTACGCATTCAGATTCAGATTGTTCAAGACTATTGGTCCTCGAGACAACAATCGATACCTTGCCGAGTTGGTTTAATAGTGTGGTGATCGAGAGAACAGAGGAAATATTTTATAGCGAGTGCAGGGGATTAAAAAACTTTCTTGTGGAATATGCCACGAAGAGATTACATGGATTGATTTCTCTTCGTGTCGAACGGTGCCATCACATGCCAAGTTTGATGAATACAACCACAACATTGGTTCCGAAAAGACCTGTGTTGGAGAAGTTGGAAGAGTTGCATCTCAATTGGTTGAATAACTTAAAAGCGTTATGCGTTGGTGACTTACCGCATGGATCTCTAGTCAATCTCAAGTTATTGGAGGTGACAGGTTGTGAAGCCTTGGAGGGCACACTTCTCCAACCGAATTTGTGGCAGAAACTTCAAAATCTGGAAGCACTTAATATTCAAGAAATGTCTCGTATGGAATATGTGTTTGAGTCTGAAGGGCCAAAACAAGAACAGGCTGCTTTTAGAAATTGGAGAGAGATGACATTGGCATATCTTGGAGAACTGAAAAGCATATGGAATGGCCCAGCTCAATATGCAATTTTCCATAATCTTAAGGTTTTGACAGTATCTGAGTGTAGGAAATTGAAGACCATTTTCACAACAGACGCATCTCACTGTCTTATGCAACTGGAACTGGAAGAGCTACGAGTATATAATTGCTCTAGTTTGGAGACAATTATTGGAGCAAACGAGGGAACATTAGAGGACAAGATCATTTTTCCGCAACTGAGATACATATCTTTGCGGAGGCTTCCAGAATTGAAAAGCTTTTATAGTGGTGGTGTTGAGTGCCCTTCATTGGAATACTTGTGTGTGCACGTGTGCCATAGCCAATTTTCTTTCTCTGCTTCTGACTTCCATAGCCAGAAGCAAGTCCGAGTAGTCCGATGA

Protein Analysis

1042

Amino Acids

117.36

Weight (kDa)

5.64

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AatII GACGTC 1 cut(s) 1425
Acc16I TGCGCA 1 cut(s) 1412
Acc36I ACCTGC 1 cut(s) 719
AccB1I GGYRCC 1 cut(s) 2382
AccI GTMKAC 1 cut(s) 446
AccIII TCCGGA 1 cut(s) 1771
AciI CCGC 4 cut(s) 297, 2513, 2957, 2980
AclWI GGATC 4 cut(s) 478, 498, 1636, 2527
AcoI YGGCCR 4 cut(s) 1437, 1468, 1511, 1875
AcsI RAATTY 8 cut(s) 166, 822, 1605, 1850, 1906, 2085, 2141, 2590
AcuI CTGAAG 2 cut(s) 960, 2688
AcvI CACGTG 1 cut(s) 3055
AcyI GRCGYC 1 cut(s) 1422
AfaI GTAC 6 cut(s) 921, 1193, 1348, 1361, 1794, 2063
AfeI AGCGCT 1 cut(s) 507
AfiI CCNNNNNNNGG 2 cut(s) 1884, 3034
AflII CTTAAG 1 cut(s) 2786
AflIII ACRYGT 1 cut(s) 3054
AhdI GACNNNNNGTC 1 cut(s) 2219
AjnI CCWGG 1 cut(s) 950
AjuI GAANNNNNNNTTGG 6 cut(s) 1205, 1237, 1596, 1628, 1968, 2000
Alw21I GWGCWC 2 cut(s) 592, 3054
Alw26I GTCTC 5 cut(s) 120, 1983, 2222, 2646, 2900
Alw44I GTGCAC 2 cut(s) 588, 3050
AlwI GGATC 4 cut(s) 478, 498, 1636, 2527
AlwNI CAGNNNCTG 3 cut(s) 251, 1666, 2804
Ama87I CYCGRG 1 cut(s) 2225
Aor13HI TCCGGA 1 cut(s) 1771
Aor51HI AGCGCT 1 cut(s) 507
AoxI GGCC 8 cut(s) 237, 670, 1437, 1468, 1511, 1875, 2672, 2755
ApaLI GTGCAC 2 cut(s) 588, 3050
ApeKI GCWGC 5 cut(s) 251, 275, 1109, 1533, 2690
ApoI RAATTY 8 cut(s) 166, 822, 1605, 1850, 1906, 2085, 2141, 2590
AseI ATTAAT 2 cut(s) 1445, 2028
Asp700I GAANNNNTTC 1 cut(s) 2103
AspLEI GCGC 2 cut(s) 508, 1413
AspS9I GGNCC 5 cut(s) 29, 954, 2221, 2672, 2756
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 5 cut(s) 278, 586, 2281, 2516, 2560
AvaI CYCGRG 1 cut(s) 2225
AvaII GGWCC 3 cut(s) 29, 954, 2221
BaeGI GKGCMC 6 cut(s) 308, 592, 1075, 2576, 3029, 3054
BalI TGGCCA 4 cut(s) 1439, 1470, 1513, 1877
BanI GGYRCC 1 cut(s) 2382
BauI CACGAG 1 cut(s) 1026
BbrPI CACGTG 1 cut(s) 3055
BbsI GAAGAC 4 cut(s) 1034, 1155, 1254, 2828
Bbv12I GWGCWC 2 cut(s) 592, 3054
BbvI GCAGC 5 cut(s) 238, 287, 1121, 1520, 2677
BccI CCATC 8 cut(s) 312, 599, 1304, 1479, 1757, 1936, 2170, 2394
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 1 cut(s) 952
BciVI GTATCC 1 cut(s) 741
BclI TGATCA 1 cut(s) 616
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 5 cut(s) 120, 1983, 2222, 2646, 2900
BfaI CTAG 8 cut(s) 377, 813, 1634, 1760, 1944, 1953, 2525, 2898
BfmI CTRYAG 1 cut(s) 1550
BfoI RGCGCY 1 cut(s) 509
BfrI CTTAAG 1 cut(s) 2786
BfuAI ACCTGC 1 cut(s) 719
BfuI GTATCC 1 cut(s) 741
BisI GCNGC 5 cut(s) 252, 276, 1110, 1534, 2691
BlsI GCNGC 5 cut(s) 253, 277, 1111, 1535, 2692
BmcAI AGTACT 1 cut(s) 1193
Bme1390I CCNGG 2 cut(s) 308, 952
Bme18I GGWCC 3 cut(s) 29, 954, 2221
BmeRI GACNNNNNGTC 1 cut(s) 2219
BmeT110I CYCGRG 1 cut(s) 2225
BmgT120I GGNCC 5 cut(s) 29, 954, 2221, 2672, 2756
BmiI GGNNCC 4 cut(s) 31, 1330, 2384, 2427
BmrFI CCNGG 2 cut(s) 308, 952
BmrI ACTGGG 1 cut(s) 2002
BmuI ACTGGG 1 cut(s) 2002
BpiI GAAGAC 4 cut(s) 1034, 1155, 1254, 2828
BpmI CTGGAG 1 cut(s) 1142
BpuEI CTTGAG 2 cut(s) 1835, 2519
BpuMI CCSGG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 2238
BsaAI YACGTR 2 cut(s) 1346, 3055
BsaBI GATNNNNATC 1 cut(s) 2971
BsaHI GRCGYC 1 cut(s) 1422
BsaJI CCNNGG 3 cut(s) 486, 570, 2565
BsaWI WCCGGW 1 cut(s) 1771
Bsc4I CCNNNNNNNGG 2 cut(s) 1884, 3034
Bse118I RCCGGY 1 cut(s) 1490
Bse1I ACTGG 6 cut(s) 268, 454, 1125, 1997, 2868, 2874
Bse3DI GCAATG 3 cut(s) 351, 916, 1751
Bse8I GATNNNNATC 1 cut(s) 2971
BseAI TCCGGA 1 cut(s) 1771
BseBI CCWGG 1 cut(s) 952
BseCI ATCGAT 1 cut(s) 2238
BseDI CCNNGG 3 cut(s) 486, 570, 2565
BseGI GGATG 5 cut(s) 306, 418, 854, 1213, 1772
BseJI GATNNNNATC 1 cut(s) 2971
BseLI CCNNNNNNNGG 2 cut(s) 1884, 3034
BseMI GCAATG 3 cut(s) 351, 916, 1751
BseMII CTCAG 5 cut(s) 195, 1820, 1874, 2796, 2954
BseNI ACTGG 6 cut(s) 268, 454, 1125, 1997, 2868, 2874
BseRI GAGGAG 2 cut(s) 365, 1652
BseSI GKGCMC 6 cut(s) 308, 592, 1075, 2576, 3029, 3054
BseXI GCAGC 5 cut(s) 238, 287, 1121, 1520, 2677
BseYI CCCAGC 3 cut(s) 827, 1131, 2758
BsgI GTGCAG 2 cut(s) 237, 2326
BshFI GGCC 8 cut(s) 239, 672, 1439, 1470, 1513, 1877, 2674, 2757
BshNI GGYRCC 1 cut(s) 2382
BshVI ATCGAT 1 cut(s) 2238
BsiHKAI GWGCWC 2 cut(s) 592, 3054
BsiHKCI CYCGRG 1 cut(s) 2225
BsiSI CCGG 3 cut(s) 308, 1491, 1772
BslFI GGGAC 3 cut(s) 15, 980, 1114
BslI CCNNNNNNNGG 2 cut(s) 1884, 3034
BsmAI GTCTC 5 cut(s) 120, 1983, 2222, 2646, 2900
BsmFI GGGAC 3 cut(s) 15, 980, 1114
BsmI GAATGC 3 cut(s) 799, 2103, 2191
BsnI GGCC 8 cut(s) 239, 672, 1439, 1470, 1513, 1877, 2674, 2757
BsoBI CYCGRG 1 cut(s) 2225
Bsp1286I GDGCHC 6 cut(s) 308, 592, 1075, 2576, 3029, 3054
Bsp13I TCCGGA 1 cut(s) 1771
Bsp143I GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
Bsp19I CCATGG 2 cut(s) 486, 570
BspACI CCGC 4 cut(s) 297, 2513, 2957, 2980
BspANI GGCC 8 cut(s) 239, 672, 1439, 1470, 1513, 1877, 2674, 2757
BspCNI CTCAG 5 cut(s) 196, 1821, 1875, 2797, 2955
BspDI ATCGAT 1 cut(s) 2238
BspEI TCCGGA 1 cut(s) 1771
BspHI TCATGA 1 cut(s) 498
BspLI GGNNCC 4 cut(s) 31, 1330, 2384, 2427
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 4 cut(s) 478, 498, 1636, 2527
BspQI GCTCTTC 4 cut(s) 129, 192, 718, 2868
BspT107I GGYRCC 1 cut(s) 2382
BspTI CTTAAG 1 cut(s) 2786
BsrDI GCAATG 3 cut(s) 351, 916, 1751
BsrFI RCCGGY 1 cut(s) 1490
BsrI ACTGG 6 cut(s) 268, 454, 1125, 1997, 2868, 2874
BssAI RCCGGY 1 cut(s) 1490
BssECI CCNNGG 3 cut(s) 486, 570, 2565
BssMI GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
BssNI GRCGYC 1 cut(s) 1422
BssSI CACGAG 1 cut(s) 1026
BssT1I CCWWGG 3 cut(s) 486, 570, 2565
Bst2BI CACGAG 1 cut(s) 1026
Bst2UI CCWGG 1 cut(s) 952
Bst4CI ACNGT 7 cut(s) 529, 1253, 1351, 1562, 2382, 2800, 2852
Bst6I CTCTTC 7 cut(s) 129, 192, 718, 2339, 2373, 2451, 2868
BstACI GRCGYC 1 cut(s) 1422
BstAFI CTTAAG 1 cut(s) 2786
BstAPI GCANNNNNTGC 1 cut(s) 251
BstBAI YACGTR 2 cut(s) 1346, 3055
BstC8I GCNNGC 4 cut(s) 1137, 1379, 1415, 2035
BstDEI CTNAG 8 cut(s) 204, 752, 1684, 1823, 1829, 1883, 2805, 2963
BstDSI CCRYGG 2 cut(s) 486, 570
BstF5I GGATG 5 cut(s) 306, 418, 854, 1213, 1772
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 508, 1413
BstKTI GATC 7 cut(s) 473, 493, 619, 1644, 2274, 2522, 2949
BstMAI GTCTC 5 cut(s) 120, 1983, 2222, 2646, 2900
BstMBI GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
BstMWI GCNNNNNNNGC 7 cut(s) 236, 251, 303, 906, 971, 1058, 2768
BstNI CCWGG 1 cut(s) 952
BstNSI RCATGY 2 cut(s) 1417, 2395
BstSCI CCNGG 2 cut(s) 306, 950
BstSFI CTRYAG 1 cut(s) 1550
BstSLI GKGCMC 6 cut(s) 308, 592, 1075, 2576, 3029, 3054
BstV1I GCAGC 5 cut(s) 238, 287, 1121, 1520, 2677
BstV2I GAAGAC 4 cut(s) 1034, 1155, 1254, 2828
BstX2I RGATCY 2 cut(s) 1641, 2519
BstXI CCANNNNNNTGG 1 cut(s) 2422
BstYI RGATCY 2 cut(s) 1641, 2519
Bsu15I ATCGAT 1 cut(s) 2238
BsuI GTATCC 1 cut(s) 741
BsuRI GGCC 8 cut(s) 239, 672, 1439, 1470, 1513, 1877, 2674, 2757
BsuTUI ATCGAT 1 cut(s) 2238
BtgI CCRYGG 2 cut(s) 486, 570
BtsCI GGATG 5 cut(s) 306, 418, 854, 1213, 1772
BtsIMutI CAGTG 4 cut(s) 56, 525, 1356, 2848
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 4 cut(s) 1137, 1379, 1415, 2035
CaiI CAGNNNCTG 3 cut(s) 251, 1666, 2804
CciI TCATGA 1 cut(s) 498
CfoI GCGC 2 cut(s) 508, 1413
Cfr10I RCCGGY 1 cut(s) 1490
Cfr13I GGNCC 5 cut(s) 29, 954, 2221, 2672, 2756
ClaI ATCGAT 1 cut(s) 2238
CseI GACGC 1 cut(s) 2849
Csp6I GTAC 6 cut(s) 920, 1192, 1347, 1360, 1793, 2062
CviQI GTAC 6 cut(s) 920, 1192, 1347, 1360, 1793, 2062
DdeI CTNAG 8 cut(s) 204, 752, 1684, 1823, 1829, 1883, 2805, 2963
DpnI GATC 7 cut(s) 472, 492, 618, 1643, 2273, 2521, 2948
DpnII GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
DraI TTTAAA 1 cut(s) 1846
DriI GACNNNNNGTC 1 cut(s) 2219
EaeI YGGCCR 4 cut(s) 1437, 1468, 1511, 1875
Eam1104I CTCTTC 7 cut(s) 129, 192, 718, 2339, 2373, 2451, 2868
Eam1105I GACNNNNNGTC 1 cut(s) 2219
EarI CTCTTC 7 cut(s) 129, 192, 718, 2339, 2373, 2451, 2868
Eco130I CCWWGG 3 cut(s) 486, 570, 2565
Eco32I GATATC 1 cut(s) 1941
Eco47I GGWCC 3 cut(s) 29, 954, 2221
Eco47III AGCGCT 1 cut(s) 507
Eco57I CTGAAG 2 cut(s) 960, 2688
Eco72I CACGTG 1 cut(s) 3055
Eco88I CYCGRG 1 cut(s) 2225
EcoRI GAATTC 2 cut(s) 822, 1906
EcoRII CCWGG 1 cut(s) 950
EcoRV GATATC 1 cut(s) 1941
EcoT14I CCWWGG 3 cut(s) 486, 570, 2565
EcoT22I ATGCAT 2 cut(s) 799, 1419
ErhI CCWWGG 3 cut(s) 486, 570, 2565
FalI AAGNNNNNCTT 6 cut(s) 660, 692, 967, 999, 1205, 1237
FaqI GGGAC 3 cut(s) 15, 980, 1114
FauI CCCGC 1 cut(s) 290
FauNDI CATATG 2 cut(s) 2150, 2747
FbaI TGATCA 1 cut(s) 616
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 5 cut(s) 252, 276, 1110, 1534, 2691
FokI GGATG 5 cut(s) 313, 405, 861, 1220, 1779
Fsp4HI GCNGC 5 cut(s) 252, 276, 1110, 1534, 2691
FspBI CTAG 8 cut(s) 377, 813, 1634, 1760, 1944, 1953, 2525, 2898
FspI TGCGCA 1 cut(s) 1412
GlaI GCGC 2 cut(s) 507, 1412
GluI GCNGC 5 cut(s) 252, 276, 1110, 1534, 2691
GsaI CCCAGC 3 cut(s) 831, 1135, 2762
GsuI CTGGAG 1 cut(s) 1142
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 8 cut(s) 239, 672, 1439, 1470, 1513, 1877, 2674, 2757
HapII CCGG 3 cut(s) 308, 1491, 1772
HgaI GACGC 1 cut(s) 2849
HhaI GCGC 2 cut(s) 508, 1413
Hin1I GRCGYC 1 cut(s) 1422
Hin6I GCGC 2 cut(s) 506, 1411
HinP1I GCGC 2 cut(s) 506, 1411
HincII GTYRAC 2 cut(s) 447, 2049
HindII GTYRAC 2 cut(s) 447, 2049
HindIII AAGCTT 4 cut(s) 1368, 1571, 1650, 3001
HpaI GTTAAC 1 cut(s) 2049
HpaII CCGG 3 cut(s) 308, 1491, 1772
HphI GGTGA 5 cut(s) 278, 586, 2281, 2516, 2560
Hpy166II GTNNAC 6 cut(s) 367, 438, 447, 590, 2049, 3052
Hpy8I GTNNAC 6 cut(s) 367, 438, 447, 590, 2049, 3052
Hpy99I CGWCG 2 cut(s) 1119, 1157
HpyAV CCTTC 9 cut(s) 134, 733, 1319, 1523, 1970, 2003, 2135, 2663, 3039
HpyCH4III ACNGT 7 cut(s) 529, 1253, 1351, 1562, 2382, 2800, 2852
HpyCH4IV ACGT 4 cut(s) 1345, 1422, 2115, 3054
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 251, 303, 906, 971, 1058, 2768
HpyF3I CTNAG 8 cut(s) 204, 752, 1684, 1823, 1829, 1883, 2805, 2963
HpySE526I ACGT 4 cut(s) 1345, 1422, 2115, 3054
Hsp92I GRCGYC 1 cut(s) 1422
HspAI GCGC 2 cut(s) 506, 1411
Kpn2I TCCGGA 1 cut(s) 1771
Ksp22I TGATCA 1 cut(s) 616
KspAI GTTAAC 1 cut(s) 2049
Kzo9I GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
LguI GCTCTTC 4 cut(s) 129, 192, 718, 2868
LmnI GCTCC 3 cut(s) 1238, 1328, 2918
Lsp1109I GCAGC 5 cut(s) 238, 287, 1121, 1520, 2677
MaeI CTAG 8 cut(s) 377, 813, 1634, 1760, 1944, 1953, 2525, 2898
MaeII ACGT 4 cut(s) 1345, 1422, 2115, 3054
MaeIII GTNAC 8 cut(s) 83, 323, 523, 893, 1280, 1351, 2504, 2548
MalI GATC 7 cut(s) 472, 492, 618, 1643, 2273, 2521, 2948
MboI GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
MfeI CAATTG 2 cut(s) 1047, 2470
MflI RGATCY 2 cut(s) 1641, 2519
MhlI GDGCHC 6 cut(s) 308, 592, 1075, 2576, 3029, 3054
MlsI TGGCCA 4 cut(s) 1439, 1470, 1513, 1877
MluNI TGGCCA 4 cut(s) 1439, 1470, 1513, 1877
MlyI GAGTC 6 cut(s) 70, 122, 443, 603, 1672, 2672
MmeI TCCRAC 6 cut(s) 1872, 1883, 2062, 2424, 2433, 2608
Mox20I TGGCCA 4 cut(s) 1439, 1470, 1513, 1877
Mph1103I ATGCAT 2 cut(s) 799, 1419
MroI TCCGGA 1 cut(s) 1771
MroXI GAANNNNTTC 1 cut(s) 2103
MscI TGGCCA 4 cut(s) 1439, 1470, 1513, 1877
MslI CAYNNNNRTG 2 cut(s) 927, 1761
Msp20I TGGCCA 4 cut(s) 1439, 1470, 1513, 1877
MspCI CTTAAG 1 cut(s) 2786
MspI CCGG 3 cut(s) 308, 1491, 1772
MspR9I CCNGG 2 cut(s) 308, 952
MunI CAATTG 2 cut(s) 1047, 2470
Mva1269I GAATGC 3 cut(s) 799, 2103, 2191
MvaI CCWGG 1 cut(s) 952
MwoI GCNNNNNNNGC 7 cut(s) 236, 251, 303, 906, 971, 1058, 2768
NciI CCSGG 1 cut(s) 308
NcoI CCATGG 2 cut(s) 486, 570
NdeI CATATG 2 cut(s) 2150, 2747
NdeII GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
NlaIV GGNNCC 4 cut(s) 31, 1330, 2384, 2427
NmeAIII GCCGAG 1 cut(s) 2274
NmuCI GTSAC 5 cut(s) 523, 893, 1351, 2504, 2548
NsbI TGCGCA 1 cut(s) 1412
NsiI ATGCAT 2 cut(s) 799, 1419
NspI RCATGY 2 cut(s) 1417, 2395
PaeI GCATGC 1 cut(s) 1417
PaeR7I CTCGAG 1 cut(s) 2225
PagI TCATGA 1 cut(s) 498
PciSI GCTCTTC 4 cut(s) 129, 192, 718, 2868
PcsI WCGNNNNNNNCGW 1 cut(s) 1395
PctI GAATGC 3 cut(s) 799, 2103, 2191
PdmI GAANNNNTTC 1 cut(s) 2103
PfeI GAWTC 6 cut(s) 664, 947, 989, 1819, 2165, 2198
PfoI TCCNGGA 1 cut(s) 950
PkrI GCNGC 5 cut(s) 253, 277, 1111, 1535, 2692
PleI GAGTC 6 cut(s) 69, 121, 442, 603, 1671, 2671
PmaCI CACGTG 1 cut(s) 3055
PmlI CACGTG 1 cut(s) 3055
PpsI GAGTC 6 cut(s) 69, 121, 442, 603, 1671, 2671
Ppu21I YACGTR 2 cut(s) 1346, 3055
PshBI ATTAAT 2 cut(s) 1445, 2028
PsiI TTATAA 1 cut(s) 773
Psp6I CCWGG 1 cut(s) 950
PspCI CACGTG 1 cut(s) 3055
PspFI CCCAGC 3 cut(s) 827, 1131, 2758
PspGI CCWGG 1 cut(s) 950
PspN4I GGNNCC 4 cut(s) 31, 1330, 2384, 2427
PspPI GGNCC 5 cut(s) 29, 954, 2221, 2672, 2756
PstNI CAGNNNCTG 3 cut(s) 251, 1666, 2804
PsuI RGATCY 2 cut(s) 1641, 2519
RsaI GTAC 6 cut(s) 921, 1193, 1348, 1361, 1794, 2063
RsaNI GTAC 6 cut(s) 920, 1192, 1347, 1360, 1793, 2062
RseI CAYNNNNRTG 2 cut(s) 927, 1761
SalI GTCGAC 1 cut(s) 445
SapI GCTCTTC 4 cut(s) 129, 192, 718, 2868
SatI GCNGC 5 cut(s) 252, 276, 1110, 1534, 2691
Sau3AI GATC 7 cut(s) 470, 490, 616, 1641, 2271, 2519, 2946
Sau96I GGNCC 5 cut(s) 29, 954, 2221, 2672, 2756
ScaI AGTACT 1 cut(s) 1193
SchI GAGTC 6 cut(s) 70, 122, 443, 603, 1672, 2672
ScrFI CCNGG 2 cut(s) 308, 952
SduI GDGCHC 6 cut(s) 308, 592, 1075, 2576, 3029, 3054
SfcI CTRYAG 1 cut(s) 1550
Sfr274I CTCGAG 1 cut(s) 2225
SinI GGWCC 3 cut(s) 29, 954, 2221
SlaI CTCGAG 1 cut(s) 2225
SmiMI CAYNNNNRTG 2 cut(s) 927, 1761
SmlI CTYRAG 4 cut(s) 1814, 2225, 2534, 2786
SmoI CTYRAG 4 cut(s) 1814, 2225, 2534, 2786
SphI GCATGC 1 cut(s) 1417
SsiI CCGC 4 cut(s) 297, 2513, 2957, 2980
SspI AATATT 3 cut(s) 1406, 2292, 2629
SspMI CTAG 8 cut(s) 377, 813, 1634, 1760, 1944, 1953, 2525, 2898
StyD4I CCNGG 2 cut(s) 306, 950
StyI CCWWGG 3 cut(s) 486, 570, 2565
TaaI ACNGT 7 cut(s) 529, 1253, 1351, 1562, 2382, 2800, 2852
TaiI ACGT 4 cut(s) 1348, 1425, 2118, 3057
TaqI TCGA 9 cut(s) 446, 1114, 1306, 1931, 2040, 2226, 2238, 2274, 2376
TatI WGTACW 2 cut(s) 919, 1191
TfiI GAWTC 6 cut(s) 664, 947, 989, 1819, 2165, 2198
TscAI CASTG 4 cut(s) 56, 532, 1356, 2855
TseFI GTSAC 5 cut(s) 523, 893, 1351, 2504, 2548
TseI GCWGC 5 cut(s) 251, 275, 1109, 1533, 2690
Tsp45I GTSAC 5 cut(s) 523, 893, 1351, 2504, 2548
TspDTI ATGAA 7 cut(s) 83, 674, 1020, 1542, 2137, 2420, 3021
TspGWI ACGGA 2 cut(s) 1170, 1780
TspRI CASTG 4 cut(s) 56, 532, 1356, 2855
Vha464I CTTAAG 1 cut(s) 2786
VneI GTGCAC 2 cut(s) 588, 3050
VpaK11BI GGWCC 3 cut(s) 29, 954, 2221
VspI ATTAAT 2 cut(s) 1445, 2028
XapI RAATTY 8 cut(s) 166, 822, 1605, 1850, 1906, 2085, 2141, 2590
XbaI TCTAGA 1 cut(s) 1759
XceI RCATGY 2 cut(s) 1417, 2395
XcmI CCANNNNNNNNNTGG 1 cut(s) 1381
XhoI CTCGAG 1 cut(s) 2225
XmiI GTMKAC 1 cut(s) 446
XmnI GAANNNNTTC 1 cut(s) 2103
XspI CTAG 8 cut(s) 377, 813, 1634, 1760, 1944, 1953, 2525, 2898
ZraI GACGTC 1 cut(s) 1423
ZrmI AGTACT 1 cut(s) 1193
Zsp2I ATGCAT 2 cut(s) 799, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.