Rmu_sc0004482.1_g000022

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004482.1
OG0000085
Physical Location & Seq
Reverse (-)
87420 .. 94907
7488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004482.1_g000022.1.cds

Sequence Viewer

Length: 4356 bp
atggccttggaatccccaacggcctctgcatcacttcctacatcagttgaatcatcggctcctgggtggaagtatgaggtgtttttgagtttcaggagtgaagacactcggcggggttttacttcttatttgtacaatgaattgcaaaacagagaaatcaagacattcatggatatcgacgaaactagtttggatagaggagaggggatttctcaaagtatcctagaggccattcaagaatcaagatttgcaatcgttgttctctcgcaaaattatgcttcttccagttggtgtttggatgaactcacacagattatgcagtgcatggaaaataaggaaacaattctgccagttttctatcacgtggatccctctgaagtgcgctctcaaaggggggtgtgcggagaggccttggctgcacatgaaaagattatgcagtgcatggaaaataaggaaacaattctgccagttttctatcacgtggatccctctgaagtgcgctctcaaaggggggtgtgcggagcggccttggctgcacatgaagagcggtttaaagatcaaaaagagaggctgatgaaatggagggacgccttgaacaaaatcagcagcatctttggctggcctgacccagctcctgtgtggaagaatgatgtgctttcgggtttcgggatggaagacactcgcaggggttttgtagcccattcagaccatgatttgccaaacacaggactacgtaaaacattcaaggatgaagaccagcttgaaaaaggggtagccctcccctctgcatctctttcctcaactgaaccaagtcttcaaccagctgttgggcagaagaatgatgagtttttgggtttcaggagaaacgacaatcataggggttttgtcacccattcaagtcatgaaatacaaaagaccaggatgactaagacattcaccgatgaagaacatcttgaaaaagggatagctgcgtctccgccttcatcagctgaacgagatcctcggccgaagtatgatgtgtttttgagtttcaggggggaagacactcgtaggggttttatagcccatttataccgtgaattgcaaaacaccagagtgattaaaactttcaaggatgacaggcaactagaaaaagggacggtcattgcttcaaatctcctaatggcaatcgaagaatcaagatttgcgatcattgtgttgtcaacaaattatgcttcttcttcatggtgtttggatgaacttgcaaagattttccaatgcatgaaagataggaacacaattttcccaattttttatgaggtggaacccactgatgtacgccatcaaaagacagagacgaggtttggaaaggctttcattaagcagcacgaaactcatggacaagacaacgagaagatgaagcaatggagtgctgctttagaaggagtggctggaatcgctgggttcccgtcaactaagtatcagacggacgtagaactaatcgaagacgttgtgaaacatttgttgagcaaagtacttgaagcacccaccggaaactttgaaacatttaaagcgacaaggcgagcaatggatgacgtgatggaggtattaaaagatgatcaagtcactgtccttggggtgcatggagtgggtggcgttggaaagacgacgatgatcaaacatgttagtgcacaagcccagaaagatgggatttttgatcatgtgattatggttgtcgtatccaaaagccctgacttgcaagaaattcaaagcacattggcacgttcgctggggatcatgagaggaaataggatgcttataatattggacgacatttgggagagttttgaactctcgagcattggaattcccagtcacaatgagcttcaaaggtgcaattctaaagttatactcaccacaaggaaaagcaatgtctgcgattctatggggtgccatgcccagatcactctcaatgttctgtcagaggaagactcttggaagttattcatggagacgtcggggaagtcctttcatgaatctaccatttcctatgatgaagcgagaaaggtagctcgagaatgctgtggtctcccaaaagcaataaaagcagctgcaaggtcacttggagataaatacatggacgaatggaatgaagcagttgtaacagcatttggagatgactgcttggacaaatggactgaaacagctccacctgtcaacttcgaagctgaggaagacgcatttcattcattggcatctgatgatgccaagtcatgcttcttgctatgctgcctattccccgaaaattatgatatcccaattgaagatctgctcaagtatgcgattggaaaaggtttgtttcaatatgatacaatgcaagaagccagagatagagcacattctgtggtcaagtacctcaaagtttctagattgcttttagacggtagaaagaagggatgcgtaaggatgactgatggcattcgaaatctggccatgtccattgcgttgcccaaagaaggcgatgggtttttagtaaaagctggttgtgaactagaggagtggccagaggcaggatggctgcaagaaatcacttccaggaacttgaactgcccaatagatgcccatgaagcctactcaacaatctcactcatgagaaacaagcttcacaagcttcccgaatatttggtatgttcaaaactccaaattttattactacaaagtaatgccggaatatgtgacatcccagaaacctttttgcaaagtcctaatccgctaagggtctttgatcttagcttcactggtgtttcgtcactaccttcatcattcagtttcctaaccaacctccaaactttgaatttggattcttgcaagaatttaagtaacatatccgttctaggaaaacttaataagaaacttaagattctgagtatgagagaacttcctcttaagaaatttccaaaacaaataggagatttgaccagtctaaggatgttggacgtcactggtagatatcttgaaactatcccatctaaagtgatatcaaggttgtatagattggaagaactgcacatgaaatttcagtttgggaagtggggaagtaaaattgaggatgcagttgatgaagaaaccaatgctgactttgatgagttgactggcttgttggatttaaacattttggaagtttacatatctgatgccgcatgcttgcctaaaagtgttgagtacatgccgaattgggcagaatttgatatatgcatcagcagacacgtcccttgcgtggctctccctcagcatgatggtttttcaagaaccttgactcttgacacaacaatcagaagcttaccgtattggtttatcagcgctgtgacagagaaggtggagaagctagagtacacagattgcgaagacttgaatgacatcgttagggaatatgaccatgggagattacttgcactgaaatatctctctgtgaacgggaaccgtaagaacttgaaagagataatcaacacaatgacacgggtttcaaataaaccggtttttgagaacttggaagagttgcatctgcaccgcttatattccctcaaggagttatgtgttggtgagctaccaactgggtctctacgcaagctcaagttcttgaaagtgcaaacttgtcgtagcttggtgaatgcacttttaccatcaacattgttgcagagactacaaaatctggaagaaatcatttgtgtaaatctggaaggaatgaaatatgtatttggatctgatgaagggcttgatccagaaaatattttcttgaggaaattgagggagatgagattgtctgctctatacgaactagtaagcatatgtaatggtcctgctccgcgtgtaatcttccagaatcttaaagttttggcaatttgcgagtgcaaggaattgaaaaatctcttcacaatagatgtagttgaatgtctttgtcagttggaagacctttccatagagcactgtcctagcttggacagagtcattgaagcaagcaatagcttgaacagcaagattaaacttctgaataagttgaagaagttagctttgatagatcttccagtgctcacaaggttctgcactgaaggtggtactattgaaactgagtgcccttcattggaatacttgtatgtggagaagtgcctccagttttcgtattttgcttaccactcggacaacaagaaccaagtccacttcaatgataaacaacatttcagttctttagtgaaaagaggtgatgtggcagtaccggagctaccgtggcagtgtcggggtgacatggtagatccgaaggtgacgtggcagtacttagctgatgtggcagtgccgaagctaatgtggctgtgggtcacctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1451

Amino Acids

164.58

Weight (kDa)

5.62

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1799
AatII GACGTC 2 cut(s) 1997, 2979
AccB1I GGYRCC 1 cut(s) 1929
AccB7I CCANNNNNTGG 1 cut(s) 827
AccBSI CCGCTC 2 cut(s) 524, 547
AccII CGCG 1 cut(s) 3844
AclWI GGATC 9 cut(s) 362, 375, 479, 492, 992, 1781, 3745, 3749, 4280
AcoI YGGCCR 3 cut(s) 1004, 2469, 2540
AcsI RAATTY 8 cut(s) 1743, 1845, 2682, 2833, 2851, 2930, 3053, 3221
AcuI CTGAAG 3 cut(s) 396, 513, 4104
AcvI CACGTG 2 cut(s) 364, 481
AcyI GRCGYC 3 cut(s) 588, 1994, 2976
AfaI GTAC 9 cut(s) 134, 1317, 1515, 2393, 3203, 3371, 4093, 4248, 4307
AfeI AGCGCT 1 cut(s) 3340
AfiI CCNNNNNNNGG 7 cut(s) 725, 827, 2495, 2549, 2964, 3256, 4117
AflII CTTAAG 2 cut(s) 2894, 2924
AflIII ACRYGT 2 cut(s) 1660, 3244
AgeI ACCGGT 1 cut(s) 3511
AhlI ACTAGT 2 cut(s) 185, 3814
AjiI CACGTC 3 cut(s) 1576, 3247, 4299
AjnI CCWGG 3 cut(s) 61, 917, 2573
AjuI GAANNNNNNNTTGG 2 cut(s) 2240, 2272
AleI CACNNNNGTG 1 cut(s) 1094
Alw21I GWGCWC 4 cut(s) 1672, 2377, 3963, 4068
Alw26I GTCTC 6 cut(s) 978, 1328, 1985, 2072, 3600, 3670
Alw44I GTGCAC 1 cut(s) 1668
AlwI GGATC 9 cut(s) 362, 375, 479, 492, 992, 1781, 3745, 3749, 4280
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 2 cut(s) 1834, 2052
Aor51HI AGCGCT 1 cut(s) 3340
AoxI GGCC 9 cut(s) 3, 21, 228, 408, 525, 620, 1004, 2469, 2540
ApaLI GTGCAC 1 cut(s) 1668
ApoI RAATTY 8 cut(s) 1743, 1845, 2682, 2833, 2851, 2930, 3053, 3221
AsiGI ACCGGT 1 cut(s) 3511
Asp700I GAANNNNTTC 3 cut(s) 342, 459, 1982
AspLEI GCGC 3 cut(s) 384, 501, 3341
AspS9I GGNCC 1 cut(s) 3833
AsuHPI GGTGA 9 cut(s) 880, 928, 1885, 3590, 3655, 4247, 4286, 4306, 4342
AsuII TTCGAA 2 cut(s) 2202, 2461
AvaI CYCGRG 2 cut(s) 1834, 2052
AvaII GGWCC 1 cut(s) 3833
BaeGI GKGCMC 2 cut(s) 1672, 4112
BalI TGGCCA 2 cut(s) 2471, 2542
BamHI GGATCC 2 cut(s) 367, 484
BanI GGYRCC 1 cut(s) 1929
BarI GAAGNNNNNNTAC 2 cut(s) 3353, 3385
BbrPI CACGTG 2 cut(s) 364, 481
Bbv12I GWGCWC 4 cut(s) 1672, 2377, 3963, 4068
BbvCI CCTCAGC 2 cut(s) 2208, 3267
BceAI ACGGC 1 cut(s) 36
BcgI CGANNNNNNTGC 2 cut(s) 3614, 3648
BciT130I CCWGG 3 cut(s) 63, 919, 2575
BciVI GTATCC 2 cut(s) 230, 1729
BclI TGATCA 3 cut(s) 1597, 1653, 1696
BcoDI GTCTC 6 cut(s) 978, 1328, 1985, 2072, 3600, 3670
BcuI ACTAGT 2 cut(s) 185, 3814
BfaI CTAG 9 cut(s) 186, 224, 1127, 2406, 2531, 2873, 3365, 3815, 3969
BfoI RGCGCY 1 cut(s) 3342
BfrI CTTAAG 2 cut(s) 2894, 2924
BfuI GTATCC 2 cut(s) 230, 1729
BglII AGATCT 2 cut(s) 2305, 4054
BmcAI AGTACT 2 cut(s) 1515, 4307
Bme1390I CCNGG 3 cut(s) 63, 919, 2575
Bme18I GGWCC 1 cut(s) 3833
BmeT110I CYCGRG 2 cut(s) 1834, 2052
BmgBI CACGTC 3 cut(s) 1576, 3247, 4299
BmgT120I GGNCC 1 cut(s) 3833
BmiI GGNNCC 7 cut(s) 60, 369, 486, 1305, 1445, 1931, 3458
BmrFI CCNGG 3 cut(s) 63, 919, 2575
BmrI ACTGGG 2 cut(s) 1845, 3600
BmuI ACTGGG 2 cut(s) 1845, 3600
BplI GAGNNNNNCTC 2 cut(s) 1955, 1987
BpmI CTGGAG 1 cut(s) 4130
Bpu10I CCTNAGC 3 cut(s) 2208, 2753, 3267
Bpu14I TTCGAA 2 cut(s) 2202, 2461
BpuEI CTTGAG 4 cut(s) 2297, 3545, 3593, 3793
BsaAI YACGTR 3 cut(s) 364, 481, 734
BsaHI GRCGYC 3 cut(s) 588, 1994, 2976
BsaI GGTCTC 2 cut(s) 2072, 3600
BsaJI CCNNGG 8 cut(s) 6, 62, 411, 528, 1001, 1612, 3415, 4259
BsaWI WCCGGW 3 cut(s) 1529, 3511, 4249
BsaXI ACNNNNNCTCC 2 cut(s) 4244, 4274
Bsc4I CCNNNNNNNGG 7 cut(s) 725, 827, 2495, 2549, 2964, 3256, 4117
Bse118I RCCGGY 1 cut(s) 3511
Bse3DI GCAATG 5 cut(s) 1143, 1409, 1572, 1915, 2478
BseBI CCWGG 3 cut(s) 63, 919, 2575
BseDI CCNNGG 8 cut(s) 6, 62, 411, 528, 1001, 1612, 3415, 4259
BseLI CCNNNNNNNGG 7 cut(s) 725, 827, 2495, 2549, 2964, 3256, 4117
BseMI GCAATG 5 cut(s) 1143, 1409, 1572, 1915, 2478
BseMII CTCAG 4 cut(s) 2199, 2894, 3281, 4095
BseRI GAGGAG 2 cut(s) 213, 2549
BseSI GKGCMC 2 cut(s) 1672, 4112
BseX3I CGGCCG 1 cut(s) 1004
BseYI CCCAGC 3 cut(s) 628, 1439, 1768
BsgI GTGCAG 5 cut(s) 402, 519, 3029, 3527, 4063
Bsh1236I CGCG 1 cut(s) 3844
Bsh1285I CGRYCG 1 cut(s) 1007
BshFI GGCC 9 cut(s) 5, 23, 230, 410, 527, 622, 1006, 2471, 2542
BshNI GGYRCC 1 cut(s) 1929
BshTI ACCGGT 1 cut(s) 3511
BsiEI CGRYCG 1 cut(s) 1007
BsiHKAI GWGCWC 4 cut(s) 1672, 2377, 3963, 4068
BsiHKCI CYCGRG 2 cut(s) 1834, 2052
BsiSI CCGG 4 cut(s) 1530, 2706, 3512, 4250
BslFI GGGAC 3 cut(s) 599, 1150, 3233
BslI CCNNNNNNNGG 7 cut(s) 725, 827, 2495, 2549, 2964, 3256, 4117
BsmAI GTCTC 6 cut(s) 978, 1328, 1985, 2072, 3600, 3670
BsmBI CGTCTC 3 cut(s) 978, 1328, 1985
BsmFI GGGAC 3 cut(s) 599, 1150, 3233
BsmI GAATGC 3 cut(s) 2063, 2457, 3652
BsnI GGCC 9 cut(s) 5, 23, 230, 410, 527, 622, 1006, 2471, 2542
Bso31I GGTCTC 2 cut(s) 2072, 3600
BsoBI CYCGRG 2 cut(s) 1834, 2052
Bsp119I TTCGAA 2 cut(s) 2202, 2461
Bsp1286I GDGCHC 5 cut(s) 1672, 2377, 3963, 4068, 4112
Bsp1407I TGTACA 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 3415
BspANI GGCC 9 cut(s) 5, 23, 230, 410, 527, 622, 1006, 2471, 2542
BspCNI CTCAG 4 cut(s) 2200, 2895, 3280, 4096
BspFNI CGCG 1 cut(s) 3844
BspHI TCATGA 4 cut(s) 901, 1776, 2011, 2628
BspLI GGNNCC 7 cut(s) 60, 369, 486, 1305, 1445, 1931, 3458
BspPI GGATC 9 cut(s) 362, 375, 479, 492, 992, 1781, 3745, 3749, 4280
BspQI GCTCTTC 1 cut(s) 537
BspT104I TTCGAA 2 cut(s) 2202, 2461
BspT107I GGYRCC 1 cut(s) 1929
BspTI CTTAAG 2 cut(s) 2894, 2924
BspTNI GGTCTC 2 cut(s) 2072, 3600
BsrBI CCGCTC 2 cut(s) 524, 547
BsrDI GCAATG 5 cut(s) 1143, 1409, 1572, 1915, 2478
BsrFI RCCGGY 1 cut(s) 3511
BsrGI TGTACA 1 cut(s) 132
BssAI RCCGGY 1 cut(s) 3511
BssECI CCNNGG 8 cut(s) 6, 62, 411, 528, 1001, 1612, 3415, 4259
BssNI GRCGYC 3 cut(s) 588, 1994, 2976
BssT1I CCWWGG 5 cut(s) 6, 411, 528, 1612, 3415
Bst2UI CCWGG 3 cut(s) 63, 919, 2575
Bst4CI ACNGT 8 cut(s) 1076, 1141, 1609, 2423, 3324, 3461, 3965, 4260
Bst6I CTCTTC 3 cut(s) 537, 3525, 3911
BstACI GRCGYC 3 cut(s) 588, 1994, 2976
BstAFI CTTAAG 2 cut(s) 2894, 2924
BstAPI GCANNNNNTGC 1 cut(s) 1914
BstAUI TGTACA 1 cut(s) 132
BstBAI YACGTR 3 cut(s) 364, 481, 734
BstBI TTCGAA 2 cut(s) 2202, 2461
BstC8I GCNNGC 6 cut(s) 620, 1563, 3181, 3185, 3605, 3994
BstDSI CCRYGG 2 cut(s) 3415, 4259
BstEII GGTNACC 1 cut(s) 4348
BstFNI CGCG 1 cut(s) 3844
BstH2I RGCGCY 1 cut(s) 3342
BstHHI GCGC 3 cut(s) 384, 501, 3341
BstMAI GTCTC 6 cut(s) 978, 1328, 1985, 2072, 3600, 3670
BstMCI CGRYCG 1 cut(s) 1007
BstNI CCWGG 3 cut(s) 63, 919, 2575
BstNSI RCATGY 3 cut(s) 1664, 3183, 3208
BstPI GGTNACC 1 cut(s) 4348
BstSCI CCNGG 3 cut(s) 61, 917, 2573
BstSLI GKGCMC 2 cut(s) 1672, 4112
BstSNI TACGTA 1 cut(s) 734
BstUI CGCG 1 cut(s) 3844
BstX2I RGATCY 7 cut(s) 367, 484, 997, 2305, 3737, 4054, 4285
BstXI CCANNNNNNTGG 1 cut(s) 1685
BstYI RGATCY 7 cut(s) 367, 484, 997, 2305, 3737, 4054, 4285
BstZI CGGCCG 1 cut(s) 1004
BsuI GTATCC 2 cut(s) 230, 1729
BsuRI GGCC 9 cut(s) 5, 23, 230, 410, 527, 622, 1006, 2471, 2542
BtgI CCRYGG 2 cut(s) 3415, 4259
BtgZI GCGATG 1 cut(s) 2514
BtrI CACGTC 3 cut(s) 1576, 3247, 4299
BtsI GCAGTG 4 cut(s) 326, 443, 4271, 4329
Cac8I GCNNGC 6 cut(s) 620, 1563, 3181, 3185, 3605, 3994
CaiI CAGNNNCTG 1 cut(s) 635
CciI TCATGA 4 cut(s) 901, 1776, 2011, 2628
CfoI GCGC 3 cut(s) 384, 501, 3341
Cfr10I RCCGGY 1 cut(s) 3511
Cfr13I GGNCC 1 cut(s) 3833
CseI GACGC 3 cut(s) 596, 960, 2225
Csp6I GTAC 9 cut(s) 133, 1316, 1514, 2392, 3202, 3370, 4092, 4247, 4306
CspAI ACCGGT 1 cut(s) 3511
CviQI GTAC 9 cut(s) 133, 1316, 1514, 2392, 3202, 3370, 4092, 4247, 4306
DraI TTTAAA 3 cut(s) 553, 1549, 3147
EaeI YGGCCR 3 cut(s) 1004, 2469, 2540
EagI CGGCCG 1 cut(s) 1004
Eam1104I CTCTTC 3 cut(s) 537, 3525, 3911
EarI CTCTTC 3 cut(s) 537, 3525, 3911
EciI GGCGGA 1 cut(s) 966
EclXI CGGCCG 1 cut(s) 1004
Eco105I TACGTA 1 cut(s) 734
Eco130I CCWWGG 5 cut(s) 6, 411, 528, 1612, 3415
Eco147I AGGCCT 1 cut(s) 410
Eco31I GGTCTC 2 cut(s) 2072, 3600
Eco32I GATATC 4 cut(s) 175, 2293, 2990, 3018
Eco47I GGWCC 1 cut(s) 3833
Eco47III AGCGCT 1 cut(s) 3340
Eco52I CGGCCG 1 cut(s) 1004
Eco57I CTGAAG 3 cut(s) 396, 513, 4104
Eco72I CACGTG 2 cut(s) 364, 481
Eco88I CYCGRG 2 cut(s) 1834, 2052
Eco91I GGTNACC 1 cut(s) 4348
EcoO65I GGTNACC 1 cut(s) 4348
EcoRI GAATTC 1 cut(s) 1845
EcoRII CCWGG 3 cut(s) 61, 917, 2573
EcoRV GATATC 4 cut(s) 175, 2293, 2990, 3018
EcoT14I CCWWGG 5 cut(s) 6, 411, 528, 1612, 3415
EcoT22I ATGCAT 2 cut(s) 1262, 3236
ErhI CCWWGG 5 cut(s) 6, 411, 528, 1612, 3415
Esp3I CGTCTC 3 cut(s) 978, 1328, 1985
FalI AAGNNNNNCTT 6 cut(s) 744, 776, 936, 968, 2240, 2272
FaqI GGGAC 3 cut(s) 599, 1150, 3233
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 1 cut(s) 3824
FbaI TGATCA 3 cut(s) 1597, 1653, 1696
FspBI CTAG 9 cut(s) 186, 224, 1127, 2406, 2531, 2873, 3365, 3815, 3969
GlaI GCGC 3 cut(s) 383, 500, 3340
GsaI CCCAGC 3 cut(s) 632, 1443, 1772
GsuI CTGGAG 1 cut(s) 4130
HaeII RGCGCY 1 cut(s) 3342
HaeIII GGCC 9 cut(s) 5, 23, 230, 410, 527, 622, 1006, 2471, 2542
HapII CCGG 4 cut(s) 1530, 2706, 3512, 4250
HgaI GACGC 3 cut(s) 596, 960, 2225
HhaI GCGC 3 cut(s) 384, 501, 3341
Hin1I GRCGYC 3 cut(s) 588, 1994, 2976
Hin6I GCGC 3 cut(s) 382, 499, 3339
HinP1I GCGC 3 cut(s) 382, 499, 3339
HincII GTYRAC 4 cut(s) 1203, 1452, 2197, 3129
HindII GTYRAC 4 cut(s) 1203, 1452, 2197, 3129
HindIII AAGCTT 3 cut(s) 2639, 2648, 3316
HpaII CCGG 4 cut(s) 1530, 2706, 3512, 4250
HphI GGTGA 9 cut(s) 880, 928, 1885, 3590, 3655, 4247, 4286, 4306, 4342
Hpy99I CGWCG 3 cut(s) 182, 1651, 1999
HpyCH4III ACNGT 8 cut(s) 1076, 1141, 1609, 2423, 3324, 3461, 3965, 4260
Hsp92I GRCGYC 3 cut(s) 588, 1994, 2976
HspAI GCGC 3 cut(s) 382, 499, 3339
Ksp22I TGATCA 3 cut(s) 1597, 1653, 1696
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 6 cut(s) 64, 521, 637, 2191, 3844, 4252
MaeI CTAG 9 cut(s) 186, 224, 1127, 2406, 2531, 2873, 3365, 3815, 3969
MbiI CCGCTC 2 cut(s) 524, 547
MfeI CAATTG 1 cut(s) 2298
MflI RGATCY 7 cut(s) 367, 484, 997, 2305, 3737, 4054, 4285
MhlI GDGCHC 5 cut(s) 1672, 2377, 3963, 4068, 4112
MlsI TGGCCA 2 cut(s) 2471, 2542
MluNI TGGCCA 2 cut(s) 2471, 2542
MlyI GAGTC 3 cut(s) 1964, 3289, 3990
MmeI TCCRAC 4 cut(s) 1618, 2952, 3120, 3921
Mox20I TGGCCA 2 cut(s) 2471, 2542
Mph1103I ATGCAT 2 cut(s) 1262, 3236
MroXI GAANNNNTTC 3 cut(s) 342, 459, 1982
MscI TGGCCA 2 cut(s) 2471, 2542
MslI CAYNNNNRTG 3 cut(s) 1094, 1665, 4197
Msp20I TGGCCA 2 cut(s) 2471, 2542
MspA1I CMGCKG 3 cut(s) 824, 989, 2090
MspCI CTTAAG 2 cut(s) 2894, 2924
MspI CCGG 4 cut(s) 1530, 2706, 3512, 4250
MspR9I CCNGG 3 cut(s) 63, 919, 2575
MunI CAATTG 1 cut(s) 2298
Mva1269I GAATGC 3 cut(s) 2063, 2457, 3652
MvaI CCWGG 3 cut(s) 63, 919, 2575
MvnI CGCG 1 cut(s) 3844
NcoI CCATGG 1 cut(s) 3415
NdeI CATATG 1 cut(s) 3824
NlaIV GGNNCC 7 cut(s) 60, 369, 486, 1305, 1445, 1931, 3458
NmeAIII GCCGAG 2 cut(s) 88, 982
NsiI ATGCAT 2 cut(s) 1262, 3236
NspI RCATGY 3 cut(s) 1664, 3183, 3208
NspV TTCGAA 2 cut(s) 2202, 2461
OliI CACNNNNGTG 1 cut(s) 1094
PaeI GCATGC 1 cut(s) 3183
PaeR7I CTCGAG 2 cut(s) 1834, 2052
PagI TCATGA 4 cut(s) 901, 1776, 2011, 2628
PceI AGGCCT 1 cut(s) 410
PciI ACATGT 1 cut(s) 1660
PciSI GCTCTTC 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 3252
PctI GAATGC 3 cut(s) 2063, 2457, 3652
PdmI GAANNNNTTC 3 cut(s) 342, 459, 1982
PflFI GACNNNGTC 1 cut(s) 3980
PflMI CCANNNNNTGG 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 2573
PinAI ACCGGT 1 cut(s) 3511
PleI GAGTC 3 cut(s) 1964, 3289, 3989
PmaCI CACGTG 2 cut(s) 364, 481
PmlI CACGTG 2 cut(s) 364, 481
PpsI GAGTC 3 cut(s) 1964, 3289, 3989
Ppu21I YACGTR 3 cut(s) 364, 481, 734
PscI ACATGT 1 cut(s) 1660
PsiI TTATAA 1 cut(s) 1799
Psp6I CCWGG 3 cut(s) 61, 917, 2573
PspCI CACGTG 2 cut(s) 364, 481
PspEI GGTNACC 1 cut(s) 4348
PspFI CCCAGC 3 cut(s) 628, 1439, 1768
PspGI CCWGG 3 cut(s) 61, 917, 2573
PspN4I GGNNCC 7 cut(s) 60, 369, 486, 1305, 1445, 1931, 3458
PspPI GGNCC 1 cut(s) 3833
PstNI CAGNNNCTG 1 cut(s) 635
PsuI RGATCY 7 cut(s) 367, 484, 997, 2305, 3737, 4054, 4285
PsyI GACNNNGTC 1 cut(s) 3980
PvuII CAGCTG 3 cut(s) 824, 989, 2090
RsaI GTAC 9 cut(s) 134, 1317, 1515, 2393, 3203, 3371, 4093, 4248, 4307
RsaNI GTAC 9 cut(s) 133, 1316, 1514, 2392, 3202, 3370, 4092, 4247, 4306
RseI CAYNNNNRTG 3 cut(s) 1094, 1665, 4197
SapI GCTCTTC 1 cut(s) 537
Sau96I GGNCC 1 cut(s) 3833
ScaI AGTACT 2 cut(s) 1515, 4307
SchI GAGTC 3 cut(s) 1964, 3289, 3990
ScrFI CCNGG 3 cut(s) 63, 919, 2575
SduI GDGCHC 5 cut(s) 1672, 2377, 3963, 4068, 4112
Sfr274I CTCGAG 2 cut(s) 1834, 2052
SfuI TTCGAA 2 cut(s) 2202, 2461
SinI GGWCC 1 cut(s) 3833
SlaI CTCGAG 2 cut(s) 1834, 2052
SmiMI CAYNNNNRTG 3 cut(s) 1094, 1665, 4197
SmlI CTYRAG 8 cut(s) 1834, 2052, 2312, 2894, 2924, 3560, 3608, 3772
SmoI CTYRAG 8 cut(s) 1834, 2052, 2312, 2894, 2924, 3560, 3608, 3772
SnaBI TACGTA 1 cut(s) 734
SpeI ACTAGT 2 cut(s) 185, 3814
SphI GCATGC 1 cut(s) 3183
SseBI AGGCCT 1 cut(s) 410
SspI AATATT 3 cut(s) 1803, 2660, 3766
SspMI CTAG 9 cut(s) 186, 224, 1127, 2406, 2531, 2873, 3365, 3815, 3969
StuI AGGCCT 1 cut(s) 410
StyD4I CCNGG 3 cut(s) 61, 917, 2573
StyI CCWWGG 5 cut(s) 6, 411, 528, 1612, 3415
TaaI ACNGT 8 cut(s) 1076, 1141, 1609, 2423, 3324, 3461, 3965, 4260
TaqI TCGA 7 cut(s) 177, 1170, 1482, 1835, 2053, 2202, 2461
TatI WGTACW 5 cut(s) 132, 1513, 3201, 3369, 4305
TauI GCSGC 2 cut(s) 527, 3179
TspGWI ACGGA 2 cut(s) 1481, 2857
Tth111I GACNNNGTC 1 cut(s) 3980
Van91I CCANNNNNTGG 1 cut(s) 827
Vha464I CTTAAG 2 cut(s) 2894, 2924
VneI GTGCAC 1 cut(s) 1668
VpaK11BI GGWCC 1 cut(s) 3833
XapI RAATTY 8 cut(s) 1743, 1845, 2682, 2833, 2851, 2930, 3053, 3221
XbaI TCTAGA 1 cut(s) 2405
XceI RCATGY 3 cut(s) 1664, 3183, 3208
XcmI CCANNNNNNNNNTGG 3 cut(s) 292, 636, 2550
XhoI CTCGAG 2 cut(s) 1834, 2052
XmnI GAANNNNTTC 3 cut(s) 342, 459, 1982
XspI CTAG 9 cut(s) 186, 224, 1127, 2406, 2531, 2873, 3365, 3815, 3969
ZraI GACGTC 2 cut(s) 1995, 2977
ZrmI AGTACT 2 cut(s) 1515, 4307
Zsp2I ATGCAT 2 cut(s) 1262, 3236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.