Rw7G030950

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000085
Physical Location & Seq
Forward (+)
44019226 .. 44032675
13450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030950.1

Sequence Viewer

Length: 3417 bp
ATGGTGATCATGGCGATCATCAAGTGGATATGGAATACAATGAAGCAGGGTCAAAATACGCTAGAAGAGGATAATGATGCCACTATGGAATCTTCATCAACAGCTCGGTCGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAAGGTATCACATCTGAATTATATTATCAACTGCAAAATGAAGGAATTCAAACATTCATGGATGACCAAGACCTTGAAGTAGGGGATAGTATTTCTCCTGAACTCCTAAAAGCAATTGAAGATTCAAGTTTCGCTATCATTGTTCTCTCGCAAAACTATGCTGTTTCAACATGGTGTTTAGAGGAACTCACCAAGATTTGTGAATGTATGAAAGACAACAAAAAAATTTTGCCACTTTTTTATTATGTCGAGCCTACTGATGTACGATATCAGAAGAAGAGTTTCAAAGATGCTTTCACTAATCATATAAACTCTGGGCGATACAAATCAAAGACCATTCTGCAGTGGAGAGATGCTTTAAACAAAGTGGCCAATTTCACCGGGTGGACAACGCAAGAATTTAGGACTGAAAGAGAACTTGTCGAACGCATTGTTGAAGATGTGTATGGTAAAGTAGCATCAATTGAAATCAACAGGAGAGATTTTCAAGAACTTGAAACAAGAAAAGAAGCCATGGAGAAGATTATGGGGGCGCTAGAAGATGATGAGGTCACTGCCGTTGGGGTCTACGGAATGGGCGGCATTGGAAAGACAACCATGGTAAAACACGTCAGTGATGAAGCCCGAAGAAAAGGGACTTTTCGTCACGTGATTATGGCAGGCATATCCCATAGCCCTGACTTGAAAAAAATTCAAGGCATATTAGCAGATCGGTTGGCCATCCAATTAAAGGACCAGACAGAAACTGGAAGAGACAATAAATTGAGCAAGGCGATAAAGAGAAAAAATAAGATGCTTATAATCTTGGATGACATATGGGAGGCAATAGATTTGTCAATCATAGGAATTCCTAGCTACAAGGAGCTTCAAAATTGCAATTCCAAAATCCTATTCACCACAAGGAGTTTGGATGTTTGCAAATCCATGGAGAGCCAACAAAACATTCTCCTCGATGTGTTATCAGATCAAGATTCTTGGAAGTTCTTTGTGACGATAACAAAATTGTCTTTTGAAATACCATCCCCTTTCTATAACGTAGCAAGGGATGTGGCAAGAGGATGTGCTGGTCTCCCAATCGCATTGGAAGCAGTTGCAAAGGCACTTAAAGAGAAAGAAATGAATGAATGGAATGAAGCAGCTCGGCGGCTAAAGGTTTCTCAACCGGCCACCCATGATGATGTGAGAGTGTTCAACTGTGTAAAGTTCAGCTATGATTGCTTGAGATCAGAAGATGCCAAGTCTTGCTTCTTGCTTTGCTGCCTTTTCCCTCAAGAATATGATATCCAAATTGAAGACTTGCTAACGTATGGGTTTGGGAAAGGACTGTTTCAAAACTGCCTAATTTGCAACACATTGAAAGACGCTAGAGACATTGTCCATTCGGAGGTCCGGTACCTTAAATCTTCTGGCTTGCTTTTGGGCGGTAAACATGATGGACAGACAAGGATGCATGATGTCATTCGACAGATGGCCATATTAATTTCATTTTCTGAAGATGGCCAACGTGCTTTGGTAAAAGCTGGCTGCAAATTACAGAAGTGGCCAAAGATTAATCCACAGGCAGGCTACTACGCAATTTCACTAATGATGAACAAAATTCCCAAGCTTCCCCAAAAGTTTGTCTGTCCAAAACTCCAGATATTATTACTGCAACACAATCCTGACGTAAAGGAGATCCCAGATTCTTTTCTGGAAAGTCTGAATGAATTAAGGGTGTTGGATCTTAGCAAAACTAGTATTTCTTCACTACCCCAAAATTTTGAGCTCCTAAACAACCTCCACACATTGTATTTAGATTCTTGTGAGCCAAGTTTTAACATATCTGTACTCGGAAAACTTGAGAAGCTTGAGATTCTTAGTCTGAGAGAATGTTGTCATGAGGGAGTTCCAAAAGAAATAGGAGACTTGGCCAATCTAAGGATGCTGGACATCACTGAATCGCGGATTGTTAAAATTGCATCTAACGTGATATCAAGGTTAAGAGAACTAGAGGAACTGTACTTGCAATGTAATTTTGAAGACTGGGGGTGTAAAGTTGAGGAAGCCGGAGAAGAAACTAATGTTCACTTTGATGAGTTGGTTGGATTGCCATATTTAACCAGTCTCAAGGTTCACATATCTCACGCAAAATTCTTGCCTACAGCTGTTGAGGTCCATCCAGATTGGGAACGCTTTGATATATGTATCAGCAACGAGGACGTGGAAGTGATAGAATCCACTTGGAAACCGCGCTTCTCTCGTGATGATTCAAGAACCTTGACTCTTAGCACAACAATTCATACCTTACCGGAGTGGTTTATCAATGTGGTGACAAAGAAAGCAGAGAAACTACAGTACAGAAAATGCAAGGCATTAAAAAACATTCTTGTGGAACATGACCGTGGGGGATTACAAGAGCTAAGACATCTCTCTATAATTGGGCGCCATGAGAATTTGAAAGAGGTTATGAATGCAGAAACATGGGATTCAAAGGAACGTGTATTTGAGAATTTGGAAGAGTTGCATCTCTTTGACGTGGACAACATCGAAAAGTTATGTGTTGGTGAATTACCATCTGGGTCGCTGTGCAATCTCATGTTATTGGATGTGAGATGGTGTCATAACCTGGTGAATGCACTTTTACAATCAAGTTTGTTGGAGAGACTGCAGTATCTGGAAAAACTTCTCTGTGCACATATGGATGGAGTGGAATTTGTGTTTGAATATGGTAAAACTACTATCTGCGAAAAGTTGCAAGAGGTGAGATTGTGCTATCTACCAAAACTAATAAAGATATGGAATGGTCATGCTCCACATTCAAGCTTCCATAATCTTAAAATTTTGGCGGTCAGCAGGTGCAAGAAGCTGACAAGTCTCTTCACATCTGATGTAACTCATTGTCTTTTGCAATTGGAAGATCTTTGGGTGTATTCTTGCCCTAACTTAAACAGAGTTGTTGAAGCAAGCGACGAAACAGAGCACAGGAGTATCATTTTTCCAAAATTGAAGAACTTGGTCTTGGTAGAACTAAAGAGGCTCAGTTCGTTCTATACTAACAGTAGTGGAAGTGCTACTTCATTGGCTGTAGAGTTCCCTTCATTGGAACACTTCCATATGCGGGGCTGCCACGATAAGTTTTCGCCCTTTGCTTCTGCCATCTGCAGGCAGAAGCAAGTCCAATTCAATATTTCTGGGGTCCTGACTCTCGCCGTATCACATATGAACTATACGATCGACTGCAGAGGAGGGGAATTAAAACGTTCAAGGATGATGGAGATCCTGTAG

Protein Analysis

1138

Amino Acids

130.38

Weight (kDa)

6.09

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 38 - 216 1e-47 TIR domain
TIR_2 PF13676 41 - 134 2.5e-16 TIR domain
NB-ARC PF00931 220 - 382 7.7e-29 NB-ARC domain
LRR_14 PF23598 580 - 857 4.5e-11 Leucine-rich repeat region
LRR_8 PF13855 594 - 652 4.4e-08 Leucine rich repeat
LRR_RPS2 PF23247 882 - 1023 1.9e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 953
AarI CACCTGC 1 cut(s) 2976
Acc36I ACCTGC 1 cut(s) 2976
Acc65I GGTACC 1 cut(s) 1545
AccB1I GGYRCC 2 cut(s) 1545, 2574
AccI GTMKAC 1 cut(s) 720
AccII CGCG 2 cut(s) 2095, 2383
AciI CCGC 7 cut(s) 732, 1297, 1575, 2095, 2381, 2978, 3250
AclI AACGTT 1 cut(s) 3391
AclWI GGATC 3 cut(s) 1822, 1881, 3403
AcoI YGGCCR 7 cut(s) 522, 870, 1317, 1623, 1651, 1694, 2061
AcuI CTGAAG 1 cut(s) 1665
AcvI CACGTG 1 cut(s) 802
AcyI GRCGYC 1 cut(s) 2575
AdeI CACNNNGTG 1 cut(s) 537
AfaI GTAC 5 cut(s) 417, 1547, 1980, 2153, 2489
AfiI CCNNNNNNNGG 7 cut(s) 883, 1537, 1715, 2070, 3232, 3250, 3294
AflIII ACRYGT 2 cut(s) 760, 2629
AhdI GACNNNNNGTC 1 cut(s) 795
AhlI ACTAGT 1 cut(s) 1886
AjiI CACGTC 3 cut(s) 763, 2353, 2668
AjnI CCWGG 1 cut(s) 2757
AjuI GAANNNNNNNTTGG 8 cut(s) 1028, 1060, 1080, 1112, 1382, 1414, 3134, 3166
AleI CACNNNNGTG 1 cut(s) 765
Alw21I GWGCWC 3 cut(s) 1920, 2827, 3114
Alw26I GTCTC 7 cut(s) 900, 1226, 1515, 2049, 2261, 2788, 3011
Alw44I GTGCAC 1 cut(s) 2823
AlwI GGATC 3 cut(s) 1822, 1881, 3403
AlwNI CAGNNNCTG 2 cut(s) 899, 2806
AoxI GGCC 7 cut(s) 522, 870, 1317, 1623, 1651, 1694, 2061
ApaLI GTGCAC 1 cut(s) 2823
ApeKI GCWGC 4 cut(s) 1289, 1410, 1677, 3255
AseI ATTAAT 2 cut(s) 1631, 1704
Asp700I GAANNNNTTC 3 cut(s) 198, 434, 2814
Asp718I GGTACC 1 cut(s) 1545
AspLEI GCGC 3 cut(s) 688, 2385, 2577
AspS9I GGNCC 5 cut(s) 140, 886, 1540, 2305, 3328
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 8 cut(s) 16, 334, 523, 1039, 2473, 2708, 2773, 2905
AvaII GGWCC 5 cut(s) 140, 886, 1540, 2305, 3328
BaeGI GKGCMC 1 cut(s) 2827
BaeI ACNNNNGTAYC 4 cut(s) 2786, 2819, 3103, 3136
BalI TGGCCA 6 cut(s) 524, 872, 1625, 1653, 1696, 2063
BanI GGYRCC 2 cut(s) 1545, 2574
BanII GRGCYC 1 cut(s) 1920
BauI CACGAG 1 cut(s) 2391
BbrPI CACGTG 1 cut(s) 802
BbsI GAAGAC 2 cut(s) 1452, 2178
Bbv12I GWGCWC 3 cut(s) 1920, 2827, 3114
BbvI GCAGC 4 cut(s) 1301, 1397, 1664, 3242
BceAI ACGGC 2 cut(s) 695, 3326
BciT130I CCWGG 1 cut(s) 2759
BclI TGATCA 1 cut(s) 6
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 7 cut(s) 900, 1226, 1515, 2049, 2261, 2788, 3011
BcuI ACTAGT 1 cut(s) 1886
BfaI CTAG 6 cut(s) 62, 689, 1005, 1518, 1887, 2141
BfmI CTRYAG 8 cut(s) 494, 2292, 2483, 2798, 3216, 3292, 3370, 3413
BfoI RGCGCY 2 cut(s) 689, 2578
BfuAI ACCTGC 1 cut(s) 2976
BglII AGATCT 1 cut(s) 3049
BisI GCNGC 6 cut(s) 733, 1290, 1298, 1411, 1678, 3256
BlsI GCNGC 6 cut(s) 734, 1291, 1299, 1412, 1679, 3257
Bme1390I CCNGG 2 cut(s) 535, 2759
Bme18I GGWCC 5 cut(s) 140, 886, 1540, 2305, 3328
BmeRI GACNNNNNGTC 1 cut(s) 795
BmgBI CACGTC 3 cut(s) 763, 2353, 2668
BmgT120I GGNCC 5 cut(s) 140, 886, 1540, 2305, 3328
BmiI GGNNCC 4 cut(s) 141, 1547, 2576, 3329
BmrFI CCNGG 2 cut(s) 535, 2759
BmrI ACTGGG 1 cut(s) 2185
BmuI ACTGGG 1 cut(s) 2185
BpiI GAAGAC 2 cut(s) 1452, 2178
BpmI CTGGAG 1 cut(s) 1772
BpuEI CTTGAG 5 cut(s) 1393, 1407, 2012, 2021, 2243
BpuMI CCSGG 1 cut(s) 535
BsaAI YACGTR 1 cut(s) 802
BsaBI GATNNNNATC 2 cut(s) 478, 3407
BsaHI GRCGYC 1 cut(s) 2575
BsaI GGTCTC 1 cut(s) 1226
BsaJI CCNNGG 4 cut(s) 666, 750, 1077, 2533
BsaWI WCCGGW 2 cut(s) 1542, 2440
BsaXI ACNNNNNCTCC 3 cut(s) 1529, 1559, 3398
Bsc4I CCNNNNNNNGG 7 cut(s) 883, 1537, 1715, 2070, 3232, 3250, 3294
Bse118I RCCGGY 1 cut(s) 1315
Bse1I ACTGG 3 cut(s) 904, 2180, 2253
Bse3DI GCAATG 1 cut(s) 2165
Bse8I GATNNNNATC 2 cut(s) 478, 3407
BseBI CCWGG 1 cut(s) 2759
BseDI CCNNGG 4 cut(s) 666, 750, 1077, 2533
BseJI GATNNNNATC 2 cut(s) 478, 3407
BseLI CCNNNNNNNGG 7 cut(s) 883, 1537, 1715, 2070, 3232, 3250, 3294
BseMI GCAATG 1 cut(s) 2165
BseMII CTCAG 2 cut(s) 2006, 3184
BseNI ACTGG 3 cut(s) 904, 2180, 2253
BseRI GAGGAG 2 cut(s) 1091, 3390
BseSI GKGCMC 1 cut(s) 2827
BseXI GCAGC 4 cut(s) 1301, 1397, 1664, 3242
Bsh1236I CGCG 2 cut(s) 2095, 2383
Bsh1285I CGRYCG 2 cut(s) 110, 3366
BshFI GGCC 7 cut(s) 524, 872, 1319, 1625, 1653, 1696, 2063
BshNI GGYRCC 2 cut(s) 1545, 2574
BsiEI CGRYCG 2 cut(s) 110, 3366
BsiHKAI GWGCWC 3 cut(s) 1920, 2827, 3114
BsiSI CCGG 5 cut(s) 534, 1316, 1543, 2199, 2441
BslFI GGGAC 1 cut(s) 802
BslI CCNNNNNNNGG 7 cut(s) 883, 1537, 1715, 2070, 3232, 3250, 3294
BsmAI GTCTC 7 cut(s) 900, 1226, 1515, 2049, 2261, 2788, 3011
BsmFI GGGAC 1 cut(s) 802
BsmI GAATGC 2 cut(s) 2608, 2770
BsnI GGCC 7 cut(s) 524, 872, 1319, 1625, 1653, 1696, 2063
Bso31I GGTCTC 1 cut(s) 1226
Bsp1286I GDGCHC 3 cut(s) 1920, 2827, 3114
Bsp19I CCATGG 3 cut(s) 666, 750, 1077
BspACI CCGC 7 cut(s) 732, 1297, 1575, 2095, 2381, 2978, 3250
BspANI GGCC 7 cut(s) 524, 872, 1319, 1625, 1653, 1696, 2063
BspCNI CTCAG 2 cut(s) 2007, 3183
BspFNI CGCG 2 cut(s) 2095, 2383
BspHI TCATGA 1 cut(s) 2029
BspLI GGNNCC 4 cut(s) 141, 1547, 2576, 3329
BspMAI CTGCAG 4 cut(s) 498, 2802, 3296, 3374
BspMI ACCTGC 1 cut(s) 2976
BspPI GGATC 3 cut(s) 1822, 1881, 3403
BspT107I GGYRCC 2 cut(s) 1545, 2574
BspTNI GGTCTC 1 cut(s) 1226
BsrDI GCAATG 1 cut(s) 2165
BsrFI RCCGGY 1 cut(s) 1315
BsrI ACTGG 3 cut(s) 904, 2180, 2253
BssAI RCCGGY 1 cut(s) 1315
BssECI CCNNGG 4 cut(s) 666, 750, 1077, 2533
BssNI GRCGYC 1 cut(s) 2575
BssSI CACGAG 1 cut(s) 2391
BssT1I CCWWGG 3 cut(s) 666, 750, 1077
Bst2BI CACGAG 1 cut(s) 2391
Bst2UI CCWGG 1 cut(s) 2759
Bst4CI ACNGT 6 cut(s) 1349, 1479, 2151, 2487, 2534, 3191
Bst6I CTCTTC 5 cut(s) 60, 425, 898, 2643, 3014
BstACI GRCGYC 1 cut(s) 2575
BstBAI YACGTR 1 cut(s) 802
BstC8I GCNNGC 6 cut(s) 814, 1565, 1675, 1717, 3097, 3296
BstDEI CTNAG 7 cut(s) 1877, 2009, 2015, 2069, 2417, 2552, 3170
BstDSI CCRYGG 4 cut(s) 666, 750, 1077, 2533
BstFNI CGCG 2 cut(s) 2095, 2383
BstH2I RGCGCY 2 cut(s) 689, 2578
BstHHI GCGC 3 cut(s) 688, 2385, 2577
BstMAI GTCTC 7 cut(s) 900, 1226, 1515, 2049, 2261, 2788, 3011
BstMCI CGRYCG 2 cut(s) 110, 3366
BstMWI GCNNNNNNNGC 3 cut(s) 1238, 1368, 1497
BstNI CCWGG 1 cut(s) 2759
BstSCI CCNGG 2 cut(s) 533, 2757
BstSFI CTRYAG 8 cut(s) 494, 2292, 2483, 2798, 3216, 3292, 3370, 3413
BstSLI GKGCMC 1 cut(s) 2827
BstUI CGCG 2 cut(s) 2095, 2383
BstV1I GCAGC 4 cut(s) 1301, 1397, 1664, 3242
BstV2I GAAGAC 2 cut(s) 1452, 2178
BstX2I RGATCY 4 cut(s) 1827, 1873, 3049, 3408
BstYI RGATCY 4 cut(s) 1827, 1873, 3049, 3408
BsuRI GGCC 7 cut(s) 524, 872, 1319, 1625, 1653, 1696, 2063
BtgI CCRYGG 4 cut(s) 666, 750, 1077, 2533
BtrI CACGTC 3 cut(s) 763, 2353, 2668
BtsI GCAGTG 2 cut(s) 503, 705
BtsIMutI CAGTG 4 cut(s) 503, 705, 772, 2085
BveI ACCTGC 1 cut(s) 2976
Cac8I GCNNGC 6 cut(s) 814, 1565, 1675, 1717, 3097, 3296
CaiI CAGNNNCTG 2 cut(s) 899, 2806
CciI TCATGA 1 cut(s) 2029
CfoI GCGC 3 cut(s) 688, 2385, 2577
Cfr10I RCCGGY 1 cut(s) 1315
Cfr13I GGNCC 5 cut(s) 140, 886, 1540, 2305, 3328
CseI GACGC 1 cut(s) 1523
CsiI ACCWGGT 1 cut(s) 2757
Csp6I GTAC 5 cut(s) 416, 1546, 1979, 2152, 2488
CspCI CAANNNNNGTGG 2 cut(s) 1182, 1217
CviQI GTAC 5 cut(s) 416, 1546, 1979, 2152, 2488
DdeI CTNAG 7 cut(s) 1877, 2009, 2015, 2069, 2417, 2552, 3170
DinI GGCGCC 1 cut(s) 2576
DraI TTTAAA 1 cut(s) 513
DraIII CACNNNGTG 1 cut(s) 537
DriI GACNNNNNGTC 1 cut(s) 795
EaeI YGGCCR 7 cut(s) 522, 870, 1317, 1623, 1651, 1694, 2061
Eam1104I CTCTTC 5 cut(s) 60, 425, 898, 2643, 3014
Eam1105I GACNNNNNGTC 1 cut(s) 795
EarI CTCTTC 5 cut(s) 60, 425, 898, 2643, 3014
Ecl136II GAGCTC 1 cut(s) 1918
Eco130I CCWWGG 3 cut(s) 666, 750, 1077
Eco24I GRGCYC 1 cut(s) 1920
Eco31I GGTCTC 1 cut(s) 1226
Eco32I GATATC 3 cut(s) 422, 1435, 2124
Eco47I GGWCC 5 cut(s) 140, 886, 1540, 2305, 3328
Eco53kI GAGCTC 1 cut(s) 1918
Eco57I CTGAAG 1 cut(s) 1665
Eco72I CACGTG 1 cut(s) 802
EcoICRI GAGCTC 1 cut(s) 1918
EcoO109I RGGNCCY 2 cut(s) 140, 3328
EcoRI GAATTC 2 cut(s) 198, 999
EcoRII CCWGG 1 cut(s) 2757
EcoRV GATATC 3 cut(s) 422, 1435, 2124
EcoT14I CCWWGG 3 cut(s) 666, 750, 1077
EcoT22I ATGCAT 1 cut(s) 1605
EcoT38I GRGCYC 1 cut(s) 1920
EgeI GGCGCC 1 cut(s) 2576
EheI GGCGCC 1 cut(s) 2576
ErhI CCWWGG 3 cut(s) 666, 750, 1077
FalI AAGNNNNNCTT 4 cut(s) 1382, 1414, 3190, 3222
FaqI GGGAC 1 cut(s) 802
FauI CCCGC 1 cut(s) 3243
FauNDI CATATG 4 cut(s) 968, 2829, 3246, 3351
FbaI TGATCA 1 cut(s) 6
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 6 cut(s) 733, 1290, 1298, 1411, 1678, 3256
FriOI GRGCYC 1 cut(s) 1920
Fsp4HI GCNGC 6 cut(s) 733, 1290, 1298, 1411, 1678, 3256
FspBI CTAG 6 cut(s) 62, 689, 1005, 1518, 1887, 2141
GlaI GCGC 3 cut(s) 687, 2384, 2576
GluI GCNGC 6 cut(s) 733, 1290, 1298, 1411, 1678, 3256
GsuI CTGGAG 1 cut(s) 1772
HaeII RGCGCY 2 cut(s) 689, 2578
HaeIII GGCC 7 cut(s) 524, 872, 1319, 1625, 1653, 1696, 2063
HapII CCGG 5 cut(s) 534, 1316, 1543, 2199, 2441
HgaI GACGC 1 cut(s) 1523
HhaI GCGC 3 cut(s) 688, 2385, 2577
Hin1I GRCGYC 1 cut(s) 2575
Hin6I GCGC 3 cut(s) 686, 2383, 2575
HinP1I GCGC 3 cut(s) 686, 2383, 2575
HindIII AAGCTT 3 cut(s) 1757, 1997, 2953
HpaII CCGG 5 cut(s) 534, 1316, 1543, 2199, 2441
HphI GGTGA 8 cut(s) 16, 334, 523, 1039, 2473, 2708, 2773, 2905
Hpy166II GTNNAC 7 cut(s) 540, 721, 1580, 2218, 2266, 2671, 2825
Hpy8I GTNNAC 7 cut(s) 540, 721, 1580, 2218, 2266, 2671, 2825
Hpy99I CGWCG 1 cut(s) 3104
HpyAV CCTTC 2 cut(s) 188, 3237
HpyCH4III ACNGT 6 cut(s) 1349, 1479, 2151, 2487, 2534, 3191
HpyF10VI GCNNNNNNNGC 3 cut(s) 1238, 1368, 1497
HpyF3I CTNAG 7 cut(s) 1877, 2009, 2015, 2069, 2417, 2552, 3170
Hsp92I GRCGYC 1 cut(s) 2575
HspAI GCGC 3 cut(s) 686, 2383, 2575
KasI GGCGCC 1 cut(s) 2574
KpnI GGTACC 1 cut(s) 1549
Ksp22I TGATCA 1 cut(s) 6
LmnI GCTCC 3 cut(s) 1015, 1923, 2947
Lsp1109I GCAGC 4 cut(s) 1301, 1397, 1664, 3242
MabI ACCWGGT 1 cut(s) 2757
MaeI CTAG 6 cut(s) 62, 689, 1005, 1518, 1887, 2141
MaeIII GTNAC 5 cut(s) 703, 797, 1141, 2461, 3022
MfeI CAATTG 3 cut(s) 267, 615, 3041
MflI RGATCY 4 cut(s) 1827, 1873, 3049, 3408
MhlI GDGCHC 3 cut(s) 1920, 2827, 3114
MlsI TGGCCA 6 cut(s) 524, 872, 1625, 1653, 1696, 2063
MluNI TGGCCA 6 cut(s) 524, 872, 1625, 1653, 1696, 2063
Mly113I GGCGCC 1 cut(s) 2575
MlyI GAGTC 2 cut(s) 2407, 3328
MmeI TCCRAC 3 cut(s) 1851, 2215, 2769
Mox20I TGGCCA 6 cut(s) 524, 872, 1625, 1653, 1696, 2063
Mph1103I ATGCAT 1 cut(s) 1605
MroXI GAANNNNTTC 3 cut(s) 198, 434, 2814
MscI TGGCCA 6 cut(s) 524, 872, 1625, 1653, 1696, 2063
MslI CAYNNNNRTG 6 cut(s) 765, 1329, 2223, 2519, 2532, 2832
Msp20I TGGCCA 6 cut(s) 524, 872, 1625, 1653, 1696, 2063
MspA1I CMGCKG 1 cut(s) 2297
MspI CCGG 5 cut(s) 534, 1316, 1543, 2199, 2441
MspR9I CCNGG 2 cut(s) 535, 2759
MunI CAATTG 3 cut(s) 267, 615, 3041
Mva1269I GAATGC 2 cut(s) 2608, 2770
MvaI CCWGG 1 cut(s) 2759
MvnI CGCG 2 cut(s) 2095, 2383
MwoI GCNNNNNNNGC 3 cut(s) 1238, 1368, 1497
NarI GGCGCC 1 cut(s) 2575
NciI CCSGG 1 cut(s) 535
NcoI CCATGG 3 cut(s) 666, 750, 1077
NdeI CATATG 4 cut(s) 968, 2829, 3246, 3351
NlaIV GGNNCC 4 cut(s) 141, 1547, 2576, 3329
NmeAIII GCCGAG 1 cut(s) 1273
NmuCI GTSAC 4 cut(s) 703, 797, 1141, 2461
NsiI ATGCAT 1 cut(s) 1605
OliI CACNNNNGTG 1 cut(s) 765
PagI TCATGA 1 cut(s) 2029
PaqCI CACCTGC 1 cut(s) 2976
PctI GAATGC 2 cut(s) 2608, 2770
PdmI GAANNNNTTC 3 cut(s) 198, 434, 2814
PflFI GACNNNGTC 1 cut(s) 1526
PkrI GCNGC 6 cut(s) 734, 1291, 1299, 1412, 1679, 3257
Ple19I CGATCG 1 cut(s) 3366
PleI GAGTC 2 cut(s) 2407, 3328
PluTI GGCGCC 1 cut(s) 2578
PmaCI CACGTG 1 cut(s) 802
PmlI CACGTG 1 cut(s) 802
PpsI GAGTC 2 cut(s) 2407, 3328
Ppu21I YACGTR 1 cut(s) 802
PpuMI RGGWCCY 2 cut(s) 140, 3328
PshBI ATTAAT 2 cut(s) 1631, 1704
PsiI TTATAA 1 cut(s) 953
Psp124BI GAGCTC 1 cut(s) 1920
Psp1406I AACGTT 1 cut(s) 3391
Psp5II RGGWCCY 2 cut(s) 140, 3328
Psp6I CCWGG 1 cut(s) 2757
PspCI CACGTG 1 cut(s) 802
PspGI CCWGG 1 cut(s) 2757
PspN4I GGNNCC 4 cut(s) 141, 1547, 2576, 3329
PspPI GGNCC 5 cut(s) 140, 886, 1540, 2305, 3328
PspPPI RGGWCCY 2 cut(s) 140, 3328
PstI CTGCAG 4 cut(s) 498, 2802, 3296, 3374
PstNI CAGNNNCTG 2 cut(s) 899, 2806
PsuI RGATCY 4 cut(s) 1827, 1873, 3049, 3408
PsyI GACNNNGTC 1 cut(s) 1526
PvuI CGATCG 1 cut(s) 3366
PvuII CAGCTG 1 cut(s) 2297
RsaI GTAC 5 cut(s) 417, 1547, 1980, 2153, 2489
RsaNI GTAC 5 cut(s) 416, 1546, 1979, 2152, 2488
RseI CAYNNNNRTG 6 cut(s) 765, 1329, 2223, 2519, 2532, 2832
SacI GAGCTC 1 cut(s) 1920
SatI GCNGC 6 cut(s) 733, 1290, 1298, 1411, 1678, 3256
Sau96I GGNCC 5 cut(s) 140, 886, 1540, 2305, 3328
SchI GAGTC 2 cut(s) 2407, 3328
ScrFI CCNGG 2 cut(s) 535, 2759
SduI GDGCHC 3 cut(s) 1920, 2827, 3114
SexAI ACCWGGT 1 cut(s) 2757
SfcI CTRYAG 8 cut(s) 494, 2292, 2483, 2798, 3216, 3292, 3370, 3413
SfoI GGCGCC 1 cut(s) 2576
SinI GGWCC 5 cut(s) 140, 886, 1540, 2305, 3328
SmiMI CAYNNNNRTG 6 cut(s) 765, 1329, 2223, 2519, 2532, 2832
SmlI CTYRAG 5 cut(s) 1372, 1422, 1991, 2000, 2258
SmoI CTYRAG 5 cut(s) 1372, 1422, 1991, 2000, 2258
SpeI ACTAGT 1 cut(s) 1886
SsiI CCGC 7 cut(s) 732, 1297, 1575, 2095, 2381, 2978, 3250
SspDI GGCGCC 1 cut(s) 2574
SspI AATATT 1 cut(s) 3319
SspMI CTAG 6 cut(s) 62, 689, 1005, 1518, 1887, 2141
SstI GAGCTC 1 cut(s) 1920
StyD4I CCNGG 2 cut(s) 533, 2757
StyI CCWWGG 3 cut(s) 666, 750, 1077
TaaI ACNGT 6 cut(s) 1349, 1479, 2151, 2487, 2534, 3191
TaqI TCGA 7 cut(s) 110, 402, 576, 1104, 1615, 2679, 3366
TaqII GACCGA 1 cut(s) 96
TatI WGTACW 3 cut(s) 1978, 2151, 2487
TauI GCSGC 2 cut(s) 735, 1300
TscAI CASTG 4 cut(s) 503, 712, 772, 2092
TseFI GTSAC 4 cut(s) 703, 797, 1141, 2461
TseI GCWGC 4 cut(s) 1289, 1410, 1677, 3255
Tsp45I GTSAC 4 cut(s) 703, 797, 1141, 2461
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 4 cut(s) 503, 712, 772, 2092
Tth111I GACNNNGTC 1 cut(s) 1526
VneI GTGCAC 1 cut(s) 2823
VpaK11BI GGWCC 5 cut(s) 140, 886, 1540, 2305, 3328
VspI ATTAAT 2 cut(s) 1631, 1704
XcmI CCANNNNNNNNNTGG 2 cut(s) 896, 1057
XmiI GTMKAC 1 cut(s) 720
XmnI GAANNNNTTC 3 cut(s) 198, 434, 2814
XspI CTAG 6 cut(s) 62, 689, 1005, 1518, 1887, 2141
Zsp2I ATGCAT 1 cut(s) 1605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.