Rw5G048400

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
84170025 .. 84178099
8075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048400.1

Sequence Viewer

Length: 5250 bp
ATGAAATCTATGTGGAGTAAAGTAAAACAACGGGTTGTGGAAATCAACACGGGTGGTTTTGAAGTATTTGAAGCAACAAGACAAGCCATGGACGAGGTTATGAATGCGCTAAATGATGACAAGGTCACTGCTGTTGGGGTCTACGGCATGGGAGGCGTTGGAAAGACAATCATGGTGGAACATGTCTGTCAACAAGCTCAGAGTAATGGCCTTTTTGATCATGTGATTATGGTTGTTGTTACCCAAAGCCCTGACTTGAGAAAAATTCAAGGCATGTTGGCAGATCAGTTGGGCGTCGGATTGCAGGAAACTACACTGAGAAAAATTCAAGGCACATTGGCAAATCAGTTTGGCACATTTGCAGATCAGTTCAGCATCAAATTGGAGGAAAGTACACAAAGTTCAAGGGCAGCAAGACTGAGTAAGGAGATAATGAGGAGAAATAAGATCCTTATAATCTTAGACGACATTTGGGAGAGAATCGATTTGTCAAGAATAGGAATACCAAGCTACAAGGAACTTCAAAATTGTAATTCCAAAGTCCTACTCACCACAAGGATAGGGAATGTCTGTCATGTCATGAGGTGCCAAGAAAATATCACCCTCAGCACCCTATCAGAAGAAGATTCTTGGACCTTATTTGTGAGAAATGCAGGAAGGTTTTCTGAATCTACAAAATTCGAGGATGTAGCGAGGAAGGTGGCTAGAGAATGTGCTGGTCTCCCAATTGCGGTGATAAAAGTTGCAAGGGCACTCGGAAATAAATTCCTGGCGGAATGGAAAGAAGCAGCTCAACGACTAGAGAAGTCACAAACTGCCAACCCTGATCATGGAGAAGATGCATTCAGATGTATAAAGTTGAGCTATGATTACCTGAAAGAAGAAGATTCCAAGTCATGCTTCTTGCTTTGTTGCCTATTTCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCAAGTATGCAATCGGGAAAGGATTCTTTCGACATGCCAAAACAATTGAAGAAGCCAGAGGAACAGCAGATTCAGTGGTCAGGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGTGAAAAAAAGGGATGTGTAAGGATGCATGATGTCATCCGGGATACGGCCCTGAATATTGCAAAATCTGAAGATGGGCATGGGTTTTTGGTAAAAGCTGGCTGTGGTTTACAGGATTGGCCACGTCAATTACATGAAGGTTACTCTGCAGTCTCACTAATGGAGAATGATATTAGCAAGCTACCCGAAGAAGAGTTGGTATGTCCAAAACTCAAGATTTTATTACTACAGGGGAATAATCATCTAGAAGAGATTCCAGAAGCTTTTTTCCAAAGTAGTAATGCACTGAGGGTCTTAGACCTTAGCCACACTAGTATTACATCACTTCCCCAATCATTCAGTCTCCTAACAAACCTTCAAGCTTTGTATTTTGAATCTTGCCCCATGATTGACTTTTCTAACCTGAGGACACTGAAGAAGCTTGAGATTCTGAGTATGAGAGGAAATGTGATCAGAGAATTGTCGAGAGAAATTGGGAATTTGACCAATCTAAGGATGTTGGACATTACCGGTGGACTTATTGGCAAAATTCCACCTAAAGTAATCTCAGAGTTGCATAGATTAGAAGAACTGTACATGGATTGTGGATTTTGGGAGTGGGGCAGTAAAGTTGAGGAAGAAGGAGAAGAAACTATTGGCTTTCATGAGAGCAGGAACCAAGTGGAACTCAATGATAAGTTATATTACTTTGGTCTAATACGCAAAAGTACTGAAAGAGAACTTGTTGAAGCCATTGCGGAATCTGCGTACTGTAAAGTAGAACCTATTGAAATCAATATGGGAGATTTTGAAGCATTTGAAGCAACAGAGCAAGACACTATGGAAATTGAGCCTACAATGCCAAAGTCGAGCAAAGATTTTGAAACAGTTGAAGCAACAACACAAGCCACCATTGGAAACTCTGAACCAGCTCAGTGGAAGCATGATGTGTTTTTGAGTTTTAGGGGTGAAGACACTCGCAAGGGTTTTCTATCCCATTTATACCATGAATTGCAAAACACGAAAGTAATTAGAACATTTAAGGATGATGAACAACTTGAGAAAGGGAAGGATATTTCTCAAAGTCTACTGACAGCAATTGAAGAATCAAGGTTTGCGATCATTGTCCTGTCAGAAAACTATGCTTCTTCTGCATGGTGTTTAGATGAACTCACGAAGATTTTTCAATGCATGGAAGGCAAGGATACAATTCTACCAATTTTTTATAATGTGGATCCTTCTGATATACGATATCAGAAGGGTCAATTTGAAGTGGCCTTTACAAAGCATGAAAGCAAGGCAAGGTATGACACAGAGAAGGTGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGAAATCTCTCTGGCTGGCATTCAAAGAACCATAAATCTGAAAGGGAACTGGTTGAAGACATTGTGAAATTTGTGCTCAAAAAAGTACCTCAAGTACCAAGGGGAGATTTTGAAGAATTTGAATCAACAAGAGAAGCCATGGATGAGGTTAAGAAGATGCTGAAAGGTGATAAGATCACCGTCATTGGGGTCTATGGAAGAGGAGGCGTTGGCAAGACAGCCATGGTGAAACATGTCAGTGCACAAGCCCAAAGAATTGGGCTTTTTGATCATGTTGTTATGGCTGTTATATCCCGAAACCCTGATTTGCAAAAAATTCAAGGCGCATTGGTAAATCAGCTGGGAATGGAATTGGTGGAGGATGAAGAAATAGAAAGAGCCAGTAGATTGAAGGAGAGGATCATGGGAGGAAAGGGGATCCTTATTATCTTGGATGACATTTGGGAGGTAATAAATTTGTCAAGCATTGGAATTCCTAGCTATGACGAGCTCCAAGGATGCAATTCCAAGGTCCTATTCACCACAAGGATCAGGAATCTTTGTCATTCTATGGGGTGCCAAGCACATATTCCTCTCAATGTCCTATCAGAGGAAGATTCTTTGAAGTTATTCATGAAGAAGTTGAGAAAGCCTTTTCATGAATCTACCATTTCCTATGATGAAGAGAGGAACATAGTTAGAGAATGTGCTGGTCTCCCAAATGTACTGGAAGAAGCTGCAAGGGCAATTGGACATAAATACGTGGAAGAATGGAATGAAGCAGTTTCAAAGGCATTTGGAGATAATGACAATGAATTGCAAAAAGCAGCTCAAAAACTAAATGCATCTCAACTTGTCCACACTAAAGATGAGACAGATACATTTAAAAGAATAGAGTTAAGCTATCATACCTTGAGGTTTGATGATGCCAAGTCATTGTTCTTGCTTTGCTGCATGTTTCCAGAAGGTTATGATATCCCAGTTGAAGAATTGTTGAAGTATGGGATTGGAAAGGGTTTGCTTCAATATTCTGACACATTGCAAGAAGCTAGAGACAGAGCAAATTCTATGGTTGCATACCTTAAAGCTTCGGGGTTGCTTTTGGATGGCAAGAAGGGATGCATAAGGATGCATTGTGGCATCCGAAATGTGGCCATGTCTATTGCTTTGTCTGAAGAAGGTCATGCTTTTTTTGTAAAAGCTGGTTGTGAATTGAAGGATTGGCCAATGGATGCTCATGAAGGCTACTCAGCAATCTCACTAATGAGAAACAAACTTAGCAAGGTTCCCGAATCTTTGGTATGTTCTGAACTCCAAATTTTACTGCTGCAAAGTAATGCTGGTATATGTGACATTCCAAAAACTTTTTTTCAATGTCCAAATGCACTAAGGGTCTTTGATCTTAGCTATACCGGTATTTCAGTACTACCCTCTTCATTCAATCTCCTCACCAACCTCCAAGCCTTGTATTTGGATTGTTGCAATAAAATAACCGACATCTCCATACTTGGGAAGCTGAAGAAAGTTGAGATTCTTAGCTTGAAAAAATTTCCTCATAAGGAGTTGCCAAAAGAGATAGGAAATTTGACCAGTCTAAGGATGCTGGACGTCACTGGTGGTTGTGTTCACAAAATTCCATCTAAAGTGCTATCGAGGTTGTATAGACTGGAAGAATTGTACATGAAATGTGAATTTGGGGACTGGGGAAGTAAAGTCGAGGAAGCAGTAGGACAAACAAATGCCGACTTTGATGAGATAATTAGCTTGTTCCGTTTAAACATTTTGAAGGTTTACATATCTGATGCAGCTTGCTTACCCAAAGAAATTGGGTACTTTGAGCCGACTTGGGTAAAATTTGACATATGCATCAGCAGAGATCTCTCTTCGGAAATGATGCTCGCTCCGGATGATCAACCTTCAAGAACCTTGACTCTTGACAGAACAATCAATGGCTTATCGGATTGGTTTATCAATGTGGTGACACAGAAAGCAGAAAAACTAGAGTACAGAGAGTGTAAGGGATTAAACAACATTCTTAAGGAATATGAGCATGGGAGATTACATGGACTCAAGCATCTCTCTGTAATTGGGTGCCATGAAAACTTGGAAGAGTTGATAAATGCAACAACATGGGATCCAAGTAAACCTGTGTTCGAGAGCTTGGAAGAGCTGCATCTCCTTCACTTGCAATGCCTCAAGGAGTTATGTGTTGGCGAATTACCTCTGGGATCTCTGAGCAATCTCAAGTTATTGAAAGTGCAAACTTGTTGTAACTTGGTGAATGCACTTTTACCACCAAAATTGTTGCAGAGACTAACAAATCTAGAAACAGTATTTTGTGTAAATATGGAACACATGAAATATTTGTTTGGCTCTGAAGGGCTGGAGCTTGGAACGACTCTCTTGACAAAATTGAGCGAGGTGAGACTCTGCGCTCTCTTCGAACTAATAAGCATCTGGAATGGTCCGGCTCCACATGCAATCTTTGGGAATCTTAAAGTTTTGGCAGTTTGCAAGTGTACAAAACTGAAAAATCTATTCCCATCCGATGTAGCTCGATCTCTTGTGCAGTTGGAAGACCTCTCTGTAGAGCGCTGCCCTAACTTGGGCATAGTTATTGAAGCATGCACCAGCTTGAAAAAGATATATCTGTTTCCAGAATTGAAGAAATTAGCTTTGATAAATCTTCCAGAGCTCACATGGTTCTGCACCGAAAATGGTACCGTTCATACCGAGTGCCCTTTATTGGAATACTTGTATGTGGATGAGTGCCCCCAGTTTGTAAACTCTGCTTCTGCTTCTGTTAAACTCAACGATCAACAACATTTGAATTCTCTAGCGAAAAGATGGGAGGACATGTATAAGCTGTAA

Protein Analysis

1749

Amino Acids

197.88

Weight (kDa)

5.62

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 28 - 219 6.7e-27 NB-ARC domain
LRR_8 PF13855 425 - 483 1.4e-10 Leucine rich repeat
LRR_14 PF23598 427 - 526 1.3e-10 Leucine-rich repeat region
TIR PF01582 663 - 832 3e-53 TIR domain
TIR_2 PF13676 666 - 763 1.9e-15 TIR domain
NB-ARC PF00931 845 - 1007 9.3e-28 NB-ARC domain
LRR_8 PF13855 1230 - 1286 3e-06 Leucine rich repeat
LRR_14 PF23598 1292 - 1355 2.3e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 1514 - 1659 9.4e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 455, 2274
AatII GACGTC 1 cut(s) 3990
Acc65I GGTACC 2 cut(s) 1038, 5099
AccB1I GGYRCC 5 cut(s) 585, 1038, 2955, 4470, 5099
AccI GTMKAC 2 cut(s) 141, 2134
AccIII TCCGGA 1 cut(s) 4282
AciI CCGC 3 cut(s) 731, 773, 1805
AcoI YGGCCR 3 cut(s) 1187, 3531, 3602
AcuI CTGAAG 5 cut(s) 1158, 1502, 3573, 3917, 4776
AcyI GRCGYC 2 cut(s) 294, 3987
AfeI AGCGCT 1 cut(s) 4973
AflII CTTAAG 1 cut(s) 4415
AflIII ACRYGT 3 cut(s) 181, 2632, 5235
AgeI ACCGGT 2 cut(s) 1577, 3791
AhlI ACTAGT 1 cut(s) 1379
AjiI CACGTC 1 cut(s) 1193
AjnI CCWGG 1 cut(s) 768
Alw21I GWGCWC 4 cut(s) 2478, 2644, 2892, 5076
Alw26I GTCTC 8 cut(s) 725, 1225, 1415, 3098, 3245, 3426, 4684, 4798
Alw44I GTGCAC 1 cut(s) 2640
Aor13HI TCCGGA 1 cut(s) 4282
Aor51HI AGCGCT 1 cut(s) 4973
AoxI GGCC 6 cut(s) 208, 1116, 1187, 2322, 3531, 3602
ApaLI GTGCAC 1 cut(s) 2640
ApeKI GCWGC 9 cut(s) 410, 788, 3116, 3206, 3330, 3706, 4184, 4549, 4974
AsiGI ACCGGT 2 cut(s) 1577, 3791
Asp700I GAANNNNTTC 4 cut(s) 661, 977, 1320, 3008
Asp718I GGTACC 2 cut(s) 1038, 5099
AspLEI GCGC 4 cut(s) 109, 2726, 4814, 4974
AspS9I GGNCC 4 cut(s) 633, 1117, 2911, 4844
AsuC2I CCSGG 1 cut(s) 1109
AsuII TTCGAA 1 cut(s) 4821
AvaII GGWCC 3 cut(s) 633, 2911, 4844
AxyI CCTNAGG 1 cut(s) 1472
BaeGI GKGCMC 4 cut(s) 754, 2644, 5120, 5153
BalI TGGCCA 3 cut(s) 1189, 3533, 3604
BamHI GGATCC 3 cut(s) 2281, 2817, 4513
BanI GGYRCC 5 cut(s) 585, 1038, 2955, 4470, 5099
BanII GRGCYC 2 cut(s) 2892, 5076
BbsI GAAGAC 5 cut(s) 933, 954, 2025, 2463, 4962
Bbv12I GWGCWC 4 cut(s) 2478, 2644, 2892, 5076
BbvCI CCTCAGC 1 cut(s) 605
BbvI GCAGC 9 cut(s) 422, 800, 3103, 3218, 3317, 3693, 4196, 4536, 4961
BccI CCATC 5 cut(s) 1136, 3479, 4024, 4930, 5220
BceAI ACGGC 2 cut(s) 160, 1131
BciT130I CCWGG 1 cut(s) 770
BciVI GTATCC 2 cut(s) 1105, 2245
BclI TGATCA 5 cut(s) 217, 826, 1518, 2668, 4288
BcnI CCSGG 1 cut(s) 1109
BcoDI GTCTC 8 cut(s) 725, 1225, 1415, 3098, 3245, 3426, 4684, 4798
BcuI ACTAGT 1 cut(s) 1379
BfmI CTRYAG 3 cut(s) 1215, 1295, 4965
BfoI RGCGCY 1 cut(s) 4975
BfrI CTTAAG 1 cut(s) 4415
BfuI GTATCC 2 cut(s) 1105, 2245
BglII AGATCT 1 cut(s) 4255
BisI GCNGC 9 cut(s) 411, 789, 3117, 3207, 3331, 3707, 4185, 4550, 4975
BlsI GCNGC 9 cut(s) 412, 790, 3118, 3208, 3332, 3708, 4186, 4551, 4976
BmcAI AGTACT 2 cut(s) 1777, 3804
Bme1390I CCNGG 2 cut(s) 770, 1109
Bme18I GGWCC 3 cut(s) 633, 2911, 4844
BmgBI CACGTC 1 cut(s) 1193
BmgT120I GGNCC 4 cut(s) 633, 1117, 2911, 4844
BmrFI CCNGG 2 cut(s) 770, 1109
BmrI ACTGGG 3 cut(s) 3353, 4090, 5149
BmuI ACTGGG 3 cut(s) 3353, 4090, 5149
BpiI GAAGAC 5 cut(s) 933, 954, 2025, 2463, 4962
BpmI CTGGAG 1 cut(s) 4784
Bpu10I CCTNAGC 2 cut(s) 605, 1370
Bpu14I TTCGAA 1 cut(s) 4821
BpuEI CTTGAG 9 cut(s) 277, 1265, 1511, 2126, 2475, 3313, 4433, 4559, 4607
BpuMI CCSGG 1 cut(s) 1109
Bsa29I ATCGAT 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 3142
BsaHI GRCGYC 2 cut(s) 294, 3987
BsaI GGTCTC 2 cut(s) 725, 3098
BsaJI CCNNGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
BsaWI WCCGGW 3 cut(s) 1577, 3791, 4282
BsaXI ACNNNNNCTCC 1 cut(s) 5222
Bse118I RCCGGY 2 cut(s) 1577, 3791
Bse1I ACTGG 9 cut(s) 2454, 2781, 3111, 3359, 3969, 3997, 4050, 4085, 5155
Bse21I CCTNAGG 1 cut(s) 1472
Bse3DI GCAATG 3 cut(s) 1800, 3416, 4574
BseAI TCCGGA 1 cut(s) 4282
BseBI CCWGG 1 cut(s) 770
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
BseMI GCAATG 3 cut(s) 1800, 3416, 4574
BseNI ACTGG 9 cut(s) 2454, 2781, 3111, 3359, 3969, 3997, 4050, 4085, 5155
BseRI GAGGAG 3 cut(s) 451, 2616, 3815
BseSI GKGCMC 4 cut(s) 754, 2644, 5120, 5153
BseXI GCAGC 9 cut(s) 422, 800, 3103, 3218, 3317, 3693, 4196, 4536, 4961
BseYI CCCAGC 1 cut(s) 2740
BsgI GTGCAG 2 cut(s) 4967, 5071
BshFI GGCC 6 cut(s) 210, 1118, 1189, 2324, 3533, 3604
BshNI GGYRCC 5 cut(s) 585, 1038, 2955, 4470, 5099
BshTI ACCGGT 2 cut(s) 1577, 3791
BshVI ATCGAT 1 cut(s) 483
BsiHKAI GWGCWC 4 cut(s) 2478, 2644, 2892, 5076
BsiSI CCGG 5 cut(s) 1108, 1578, 3792, 4283, 4847
BslFI GGGAC 1 cut(s) 4091
BsmAI GTCTC 8 cut(s) 725, 1225, 1415, 3098, 3245, 3426, 4684, 4798
BsmFI GGGAC 1 cut(s) 4091
BsmI GAATGC 4 cut(s) 109, 842, 2419, 4666
BsnI GGCC 6 cut(s) 210, 1118, 1189, 2324, 3533, 3604
Bso31I GGTCTC 2 cut(s) 725, 3098
Bsp119I TTCGAA 1 cut(s) 4821
Bsp1286I GDGCHC 7 cut(s) 754, 2478, 2644, 2892, 5076, 5120, 5153
Bsp13I TCCGGA 1 cut(s) 4282
Bsp1407I TGTACA 3 cut(s) 1641, 4056, 4898
Bsp19I CCATGG 3 cut(s) 87, 2538, 2622
BspACI CCGC 3 cut(s) 731, 773, 1805
BspANI GGCC 6 cut(s) 210, 1118, 1189, 2324, 3533, 3604
BspDI ATCGAT 1 cut(s) 483
BspEI TCCGGA 1 cut(s) 4282
BspHI TCATGA 5 cut(s) 579, 1711, 3012, 3037, 3616
BspMAI CTGCAG 1 cut(s) 1219
BspQI GCTCTTC 1 cut(s) 4539
BspT104I TTCGAA 1 cut(s) 4821
BspT107I GGYRCC 5 cut(s) 585, 1038, 2955, 4470, 5099
BspTI CTTAAG 1 cut(s) 4415
BspTNI GGTCTC 2 cut(s) 725, 3098
BsrDI GCAATG 3 cut(s) 1800, 3416, 4574
BsrFI RCCGGY 2 cut(s) 1577, 3791
BsrGI TGTACA 3 cut(s) 1641, 4056, 4898
BsrI ACTGG 9 cut(s) 2454, 2781, 3111, 3359, 3969, 3997, 4050, 4085, 5155
BssAI RCCGGY 2 cut(s) 1577, 3791
BssECI CCNNGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
BssNI GRCGYC 2 cut(s) 294, 3987
BssT1I CCWWGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 6 cut(s) 1641, 1820, 1936, 2581, 4711, 5104
Bst6I CTCTTC 9 cut(s) 1254, 1311, 2593, 3057, 3817, 4267, 4482, 4539, 4823
BstACI GRCGYC 2 cut(s) 294, 3987
BstAFI CTTAAG 1 cut(s) 4415
BstAUI TGTACA 3 cut(s) 1641, 4056, 4898
BstBAI YACGTR 1 cut(s) 3142
BstBI TTCGAA 1 cut(s) 4821
BstC8I GCNNGC 7 cut(s) 1058, 1168, 1247, 2417, 4189, 4278, 5005
BstDSI CCRYGG 3 cut(s) 87, 2538, 2622
BstENI CCTNNNNNAGG 1 cut(s) 2987
BstH2I RGCGCY 1 cut(s) 4975
BstHHI GCGC 4 cut(s) 109, 2726, 4814, 4974
BstMAI GTCTC 8 cut(s) 725, 1225, 1415, 3098, 3245, 3426, 4684, 4798
BstMWI GCNNNNNNNGC 8 cut(s) 153, 1811, 1868, 1906, 2198, 2244, 3122, 4856
BstNI CCWGG 1 cut(s) 770
BstNSI RCATGY 8 cut(s) 185, 277, 992, 2636, 3337, 4859, 5007, 5239
BstSCI CCNGG 2 cut(s) 768, 1107
BstSFI CTRYAG 3 cut(s) 1215, 1295, 4965
BstSLI GKGCMC 4 cut(s) 754, 2644, 5120, 5153
BstV1I GCAGC 9 cut(s) 422, 800, 3103, 3218, 3317, 3693, 4196, 4536, 4961
BstV2I GAAGAC 5 cut(s) 933, 954, 2025, 2463, 4962
BstX2I RGATCY 6 cut(s) 447, 2281, 2817, 4255, 4513, 4607
BstXI CCANNNNNNTGG 2 cut(s) 1983, 2657
BstYI RGATCY 6 cut(s) 447, 2281, 2817, 4255, 4513, 4607
Bsu15I ATCGAT 1 cut(s) 483
Bsu36I CCTNAGG 1 cut(s) 1472
BsuI GTATCC 2 cut(s) 1105, 2245
BsuRI GGCC 6 cut(s) 210, 1118, 1189, 2324, 3533, 3604
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 3 cut(s) 87, 2538, 2622
BtrI CACGTC 1 cut(s) 1193
BtsI GCAGTG 2 cut(s) 126, 2381
BtsIMutI CAGTG 9 cut(s) 126, 314, 1035, 1352, 1478, 1988, 2381, 2644, 3990
Cac8I GCNNGC 7 cut(s) 1058, 1168, 1247, 2417, 4189, 4278, 5005
CciI TCATGA 5 cut(s) 579, 1711, 3012, 3037, 3616
CfoI GCGC 4 cut(s) 109, 2726, 4814, 4974
Cfr10I RCCGGY 2 cut(s) 1577, 3791
Cfr13I GGNCC 4 cut(s) 633, 1117, 2911, 4844
ClaI ATCGAT 1 cut(s) 483
CseI GACGC 1 cut(s) 283
CspAI ACCGGT 2 cut(s) 1577, 3791
CspCI CAANNNNNGTGG 6 cut(s) 34, 69, 156, 191, 4662, 4697
DraI TTTAAA 2 cut(s) 3265, 4155
EaeI YGGCCR 3 cut(s) 1187, 3531, 3602
Eam1104I CTCTTC 9 cut(s) 1254, 1311, 2593, 3057, 3817, 4267, 4482, 4539, 4823
EarI CTCTTC 9 cut(s) 1254, 1311, 2593, 3057, 3817, 4267, 4482, 4539, 4823
EciI GGCGGA 1 cut(s) 788
Ecl136II GAGCTC 2 cut(s) 2890, 5074
Eco130I CCWWGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
Eco24I GRGCYC 2 cut(s) 2892, 5076
Eco31I GGTCTC 2 cut(s) 725, 3098
Eco32I GATATC 2 cut(s) 2300, 3355
Eco47I GGWCC 3 cut(s) 633, 2911, 4844
Eco47III AGCGCT 1 cut(s) 4973
Eco53kI GAGCTC 2 cut(s) 2890, 5074
Eco57I CTGAAG 5 cut(s) 1158, 1502, 3573, 3917, 4776
Eco81I CCTNAGG 1 cut(s) 1472
EcoICRI GAGCTC 2 cut(s) 2890, 5074
EcoNI CCTNNNNNAGG 1 cut(s) 2987
EcoO109I RGGNCCY 1 cut(s) 2911
EcoRI GAATTC 2 cut(s) 2871, 5209
EcoRII CCWGG 1 cut(s) 768
EcoRV GATATC 2 cut(s) 2300, 3355
EcoT14I CCWWGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
EcoT22I ATGCAT 7 cut(s) 844, 1098, 2240, 3226, 3503, 3513, 4247
EcoT38I GRGCYC 2 cut(s) 2892, 5076
ErhI CCWWGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 1 cut(s) 4091
FauNDI CATATG 1 cut(s) 4241
FbaI TGATCA 5 cut(s) 217, 826, 1518, 2668, 4288
FblI GTMKAC 2 cut(s) 141, 2134
Fnu4HI GCNGC 9 cut(s) 411, 789, 3117, 3207, 3331, 3707, 4185, 4550, 4975
FriOI GRGCYC 2 cut(s) 2892, 5076
Fsp4HI GCNGC 9 cut(s) 411, 789, 3117, 3207, 3331, 3707, 4185, 4550, 4975
GlaI GCGC 4 cut(s) 108, 2725, 4813, 4973
GluI GCNGC 9 cut(s) 411, 789, 3117, 3207, 3331, 3707, 4185, 4550, 4975
GsaI CCCAGC 1 cut(s) 2744
GsuI CTGGAG 1 cut(s) 4784
HaeII RGCGCY 1 cut(s) 4975
HaeIII GGCC 6 cut(s) 210, 1118, 1189, 2324, 3533, 3604
HapII CCGG 5 cut(s) 1108, 1578, 3792, 4283, 4847
HgaI GACGC 1 cut(s) 283
HhaI GCGC 4 cut(s) 109, 2726, 4814, 4974
Hin1I GRCGYC 2 cut(s) 294, 3987
Hin6I GCGC 4 cut(s) 107, 2724, 4812, 4972
HinP1I GCGC 4 cut(s) 107, 2724, 4812, 4972
HincII GTYRAC 1 cut(s) 191
HindII GTYRAC 1 cut(s) 191
HindIII AAGCTT 4 cut(s) 1329, 1428, 1487, 3465
HpaII CCGG 5 cut(s) 1108, 1578, 3792, 4283, 4847
Hpy99I CGWCG 1 cut(s) 299
HpyCH4III ACNGT 6 cut(s) 1641, 1820, 1936, 2581, 4711, 5104
HpyCH4IV ACGT 3 cut(s) 1192, 3141, 3987
HpyF10VI GCNNNNNNNGC 8 cut(s) 153, 1811, 1868, 1906, 2198, 2244, 3122, 4856
HpySE526I ACGT 3 cut(s) 1192, 3141, 3987
Hsp92I GRCGYC 2 cut(s) 294, 3987
HspAI GCGC 4 cut(s) 107, 2724, 4812, 4972
Kpn2I TCCGGA 1 cut(s) 4282
KpnI GGTACC 2 cut(s) 1042, 5103
Ksp22I TGATCA 5 cut(s) 217, 826, 1518, 2668, 4288
LguI GCTCTTC 1 cut(s) 4539
LmnI GCTCC 4 cut(s) 2895, 4285, 4765, 4855
Lsp1109I GCAGC 9 cut(s) 422, 800, 3103, 3218, 3317, 3693, 4196, 4536, 4961
MaeII ACGT 3 cut(s) 1192, 3141, 3987
MaeIII GTNAC 8 cut(s) 124, 238, 807, 1208, 3728, 3988, 4357, 4649
MfeI CAATTG 4 cut(s) 726, 999, 2145, 3126
MflI RGATCY 6 cut(s) 447, 2281, 2817, 4255, 4513, 4607
MhlI GDGCHC 7 cut(s) 754, 2478, 2644, 2892, 5076, 5120, 5153
MlsI TGGCCA 3 cut(s) 1189, 3533, 3604
MluNI TGGCCA 3 cut(s) 1189, 3533, 3604
MlyI GAGTC 4 cut(s) 4303, 4440, 4771, 4800
MmeI TCCRAC 4 cut(s) 139, 277, 1548, 4932
Mox20I TGGCCA 3 cut(s) 1189, 3533, 3604
Mph1103I ATGCAT 7 cut(s) 844, 1098, 2240, 3226, 3503, 3513, 4247
MroI TCCGGA 1 cut(s) 4282
MroXI GAANNNNTTC 4 cut(s) 661, 977, 1320, 3008
MscI TGGCCA 3 cut(s) 1189, 3533, 3604
MslI CAYNNNNRTG 3 cut(s) 847, 2637, 3506
Msp20I TGGCCA 3 cut(s) 1189, 3533, 3604
MspA1I CMGCKG 1 cut(s) 2740
MspCI CTTAAG 1 cut(s) 4415
MspI CCGG 5 cut(s) 1108, 1578, 3792, 4283, 4847
MspR9I CCNGG 2 cut(s) 770, 1109
MssI GTTTAAAC 1 cut(s) 4155
MunI CAATTG 4 cut(s) 726, 999, 2145, 3126
Mva1269I GAATGC 4 cut(s) 109, 842, 2419, 4666
MvaI CCWGG 1 cut(s) 770
MwoI GCNNNNNNNGC 8 cut(s) 153, 1811, 1868, 1906, 2198, 2244, 3122, 4856
NciI CCSGG 1 cut(s) 1109
NcoI CCATGG 3 cut(s) 87, 2538, 2622
NdeI CATATG 1 cut(s) 4241
NmuCI GTSAC 5 cut(s) 124, 807, 3728, 3988, 4357
NsiI ATGCAT 7 cut(s) 844, 1098, 2240, 3226, 3503, 3513, 4247
NspI RCATGY 8 cut(s) 185, 277, 992, 2636, 3337, 4859, 5007, 5239
NspV TTCGAA 1 cut(s) 4821
PaeI GCATGC 1 cut(s) 5007
PagI TCATGA 5 cut(s) 579, 1711, 3012, 3037, 3616
PciI ACATGT 3 cut(s) 181, 2632, 5235
PciSI GCTCTTC 1 cut(s) 4539
PctI GAATGC 4 cut(s) 109, 842, 2419, 4666
PdmI GAANNNNTTC 4 cut(s) 661, 977, 1320, 3008
PflFI GACNNNGTC 1 cut(s) 122
PfoI TCCNGGA 1 cut(s) 1107
PinAI ACCGGT 2 cut(s) 1577, 3791
PkrI GCNGC 9 cut(s) 412, 790, 3118, 3208, 3332, 3708, 4186, 4551, 4976
PleI GAGTC 4 cut(s) 4303, 4440, 4771, 4800
PmeI GTTTAAAC 1 cut(s) 4155
PpsI GAGTC 4 cut(s) 4303, 4440, 4771, 4800
Ppu21I YACGTR 1 cut(s) 3142
PpuMI RGGWCCY 1 cut(s) 2911
PscI ACATGT 3 cut(s) 181, 2632, 5235
PsiI TTATAA 2 cut(s) 455, 2274
Psp124BI GAGCTC 2 cut(s) 2892, 5076
Psp5II RGGWCCY 1 cut(s) 2911
Psp6I CCWGG 1 cut(s) 768
PspFI CCCAGC 1 cut(s) 2740
PspGI CCWGG 1 cut(s) 768
PspPI GGNCC 4 cut(s) 633, 1117, 2911, 4844
PspPPI RGGWCCY 1 cut(s) 2911
PsrI GAACNNNNNNTAC 2 cut(s) 385, 417
PstI CTGCAG 1 cut(s) 1219
PsuI RGATCY 6 cut(s) 447, 2281, 2817, 4255, 4513, 4607
PsyI GACNNNGTC 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 2740
RseI CAYNNNNRTG 3 cut(s) 847, 2637, 3506
SacI GAGCTC 2 cut(s) 2892, 5076
SapI GCTCTTC 1 cut(s) 4539
SatI GCNGC 9 cut(s) 411, 789, 3117, 3207, 3331, 3707, 4185, 4550, 4975
Sau96I GGNCC 4 cut(s) 633, 1117, 2911, 4844
ScaI AGTACT 2 cut(s) 1777, 3804
SchI GAGTC 4 cut(s) 4303, 4440, 4771, 4800
ScrFI CCNGG 2 cut(s) 770, 1109
SduI GDGCHC 7 cut(s) 754, 2478, 2644, 2892, 5076, 5120, 5153
SfcI CTRYAG 3 cut(s) 1215, 1295, 4965
SfuI TTCGAA 1 cut(s) 4821
SinI GGWCC 3 cut(s) 633, 2911, 4844
SmiMI CAYNNNNRTG 3 cut(s) 847, 2637, 3506
SpeI ACTAGT 1 cut(s) 1379
SphI GCATGC 1 cut(s) 5007
SsiI CCGC 3 cut(s) 731, 773, 1805
SspI AATATT 3 cut(s) 1126, 3407, 4742
SstI GAGCTC 2 cut(s) 2892, 5076
StyD4I CCNGG 2 cut(s) 768, 1107
StyI CCWWGG 6 cut(s) 87, 2498, 2538, 2622, 2893, 2907
TaaI ACNGT 6 cut(s) 1641, 1820, 1936, 2581, 4711, 5104
TaiI ACGT 3 cut(s) 1195, 3144, 3990
TatI WGTACW 8 cut(s) 392, 1641, 1775, 3103, 3802, 4056, 4383, 4898
TscAI CASTG 9 cut(s) 133, 321, 1035, 1359, 1485, 1988, 2381, 2644, 3997
TseFI GTSAC 5 cut(s) 124, 807, 3728, 3988, 4357
TseI GCWGC 9 cut(s) 410, 788, 3116, 3206, 3330, 3706, 4184, 4549, 4974
Tsp45I GTSAC 5 cut(s) 124, 807, 3728, 3988, 4357
TspGWI ACGGA 1 cut(s) 4139
TspRI CASTG 9 cut(s) 133, 321, 1035, 1359, 1485, 1988, 2381, 2644, 3997
Tth111I GACNNNGTC 1 cut(s) 122
Vha464I CTTAAG 1 cut(s) 4415
VneI GTGCAC 1 cut(s) 2640
VpaK11BI GGWCC 3 cut(s) 633, 2911, 4844
XagI CCTNNNNNAGG 1 cut(s) 2987
XbaI TCTAGA 2 cut(s) 1312, 4702
XceI RCATGY 8 cut(s) 185, 277, 992, 2636, 3337, 4859, 5007, 5239
XcmI CCANNNNNNNNNTGG 1 cut(s) 5076
XmiI GTMKAC 2 cut(s) 141, 2134
XmnI GAANNNNTTC 4 cut(s) 661, 977, 1320, 3008
ZraI GACGTC 1 cut(s) 3988
ZrmI AGTACT 2 cut(s) 1777, 3804
Zsp2I ATGCAT 7 cut(s) 844, 1098, 2240, 3226, 3503, 3513, 4247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.