Rw5G039550

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69763386 .. 69777858
14473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039550.1

Sequence Viewer

Length: 3387 bp
ATGGCCTCAAGCAGCCAAACAGCATCCTCATCTCATCCTTCATCATCTGCTCCTCGGAGGAAGTACGATGTTTTTCTGAATTTCCGGGGTGAAGATACTCGCAGGACTATTGTCCCCTTTTTATACCGGGAATTGCAAAACAGAGAAATTGAAACATTCATGGATGACACAGGGCTTAGCAGAGGGAGGTCTATTGCTCCAAGTCTGCTAACAGCAATCCAAGAATCCAGGTCAGCGATCGTTGTTCTTTCACCAAACTATGCGTATTCTAGATGGTGTTTGGACGAATTGACAATGATCATTCAGTGCATGGAAGATAAGAGCACAACAGTTATCCCAATCTTTTATGATGTGGATCCCTCTGATGTGCGACATCAGAGAGGGAGTTTTGCAACTGCCTTCACTAGGCACCAAGAAAGGCTTGCGCAAGACGATGACTACCCAAATAAGTTGAACCAGTGGAGAGTTGCTTTAACAAAAGTTGCAGGTCTCTCTGGCTTGGAATCAAACAAATATAGTTCTGACCTACAACTTGTCCAAGACATTGTGGAAACCGTGTGCAGTAAATTACGACCTATTGATATTGAGTGGGCATTGTCCTCTACCGAAGATTATGTAGCATTTGAAGCTACAAGACGAGCCATGGACAAGGTAATCAGGGCTCTTCAAGATGATGAGATCACTGCCATTGGGGTCTATGGTATGGGAGGCGTTGGCAAGACGAGTATGGTGAAACATGTCGGTGCACAAGCCCGGAAAATTGGGCTTTTTAATCATGTGATTATGGCTGTCGTATCCCAAACACCTAATTTGAGAAATATTCAAGGCACACTGGCTGATCTGCTTGGCTTAAAGTTGCAGGAGGAGACAGATATTGGAAGAGCCGCTAGAATAGTGGAGAAGATATTAAGAGGATATAAGATCCTTATAATCTTGGACGACATTTGGAAGACTATAGATTTGTCTAGTATCGGAATACCGAACCGTACAGAGCTTCAAAGGCGCAATTCAAAGATCCTACTCACTACAAGGAGGGTGAATGTCTGTCATTCCATGGGGAGCCAAGCAAGCATTCCTCTCCGAGTTTTGTCAGAAGATGATGCTTGGGACCTACCAAAAAAAAAAAAATATGAATCACCCAACTTTTATGACGTAGCGAGGACTGTAGCTAAAGAATGTGCTGGTCTCCCGGTTGCATTGGTAGCAGTTGCAAGGGCACTTGGAGATAAGGACTTGGATGATTGGACAGAAGCAGCTCGACAACTAAAGACTGCTCAACCTGCCAACTATGAAGATGAGAGAGATGTGTTCAGATGTATAAAGTTAAGCTATGATTACTTGAAACCTGATGATGACAACATTGCCAAGTTATTCTTCTTGCTTTGTTGTCTATTCCCTGAAGATTTTGATATCGAGGTCGAAGACTTGTTGGATGCCCACACAATACAAGACGCCAGAGCCAAATCCCATTCGGTGGTTAAGTACCTGAAAGCTTCTAGCTTGCTTTTGGACAGTGCATACCCCGGATTCGTGAAGATGCATGATGTCATTCGAGATGTGGCCATAGAAATTTCTTCATCGAAAGATGGCCCACGGTTTTTGGTTAGAGCTGGTTGTGAATTAAGGGATTGGCCAGCGATTAATATTGCACATGATGGCTACTCTGCAATCTCGCTAATGAGGAATGAAATTCACCAGCTTCCCAAAACGTTGAGAAATAGTATAAATGAGATTCCAAACCCTTTCTTTCAAAGTCCCAATGAATTGAGGGTCTTAGATCTCAGCGAGACTAGTATTTCGTTTCTACCCTCATCATCAAGCCTTCTAACCAACCTCCAGGCTTTGTATTTAGATGAATGCAAGAGAATAGTTGATATGTCCATACTCGGAAAACTGAAGAACCTTCAGATTCTTAGTATGAAGAACCTTGACATTATTAATACCGGAGGAACTCGTCAAAAAGCATTGCCGAAAGAAATAGGACGCTTAACCAAGCTTATGATGTTGGATGTTACGGATGCATGTTTTGAAACAGTTCCATCTAATGTGATATCAAACTTGAATGGATTAGAAGAATTGTACATGAAATGTGACTTTTCTGACTGGGGCAGTAGAATAGTTGAGGGCAGCAGTTTATTTTCTTCTCTTTTTGGCACTGCCAAGAATGCTAGCTTTGATGAGTTAACAAGCTTATCACGCTTAAGCAGTTTGAAAGTTCACATATCTGACGTAGAATGCTTGCCCCGAAAAGTTAGATTCAATCCCAATTGGTTCAACTTTGATATATGTATCAGCAGAAAAGAATTTATGAGACAGTCCTATTACGGACGCTTTTTCGAGAGGAAACTAACTGTCAAGTCGGATCCACGCCAACATCTCTTTCGGTATGTGACTCTAAACACAAGCACGGAGAGTTTACCAGATTGGTTTATCGAGGTGGTGACAGAGAAAGCAGAGATGCTGGAGTACATAGGGTACAGTGGCTTAAAGGACATTCTTGTGGAATACAACCTTGGGAGATTACATGTGCTGAAGTGTCTTGCTGTAGTTGAGTGCCATAATGTGTGTGGAGGGTTGATGAACTCAGGAAGAAGGGTTCTAAATAAACCTGTGTTTGAGAGCTTGGAAGAGTTTTATCTAGAAGGGGTCCATGGCCTGCGGTGCTTATGTGTTGCTGAGTTACCGCCTGGGTCTCTGCGCAATCTAAAGTTGTTAAATGTGAGAAATTGCCTGGAATTGGTGGGTGCACTTTTACGATCAAATTTGTTGCAAAGGCTACAAAATCTGGAAATTCTACATTGTGAGTACACGGAGGATGCAAAAGAAACCGAATTGGAATCTGTGTTTGGATTTGGATGGCTGGAAACAGAACAAATTGTCTTACCAAAATTGAGAGAGATCACATTATGTTATCTTGTGAACCTAAAAGCCATATGCCATGGTCATCCTCCCAATGGAGTCTTCCGTAATCTTAACAGTCTGGCAATTCGTGGCTGCGACAAACTGAAATATCTGTTCGAGGACAAAGTAGCTCAGTCTCTTCCCCAATTGGAACTAGTTTGGGTGGAGAAGTGCTCTGACTTGGTAACGCTTATTAAACCAAGCCAGGAACTCCTTTTATTTCCAAAATTGAAGGAAATATATTTAAATAGTCTTCCAAATCTCACATCGATCTACACAAACGATGGGGTTATGTGCTCTTCATTGGAACACGTGTACGTGCACAGTTGTCCCCGACTTTCAAAGTTTGTTGCTTCTGACCTTCTCAGCGGGAACCGGGTCCAAGTCAATGATGAAGGACATTTTGCGATTATATTTGAAAGATTGTTCCACCGCCAATTCAATCCAGAAGAGTTTCCTCAGTTCTTCTCCAAAGAAGGAAAGTGA

Protein Analysis

1128

Amino Acids

127.92

Weight (kDa)

6.16

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 193 6.4e-50 TIR domain
TIR_2 PF13676 24 - 121 1.8e-14 TIR domain
NB-ARC PF00931 214 - 377 5.7e-30 NB-ARC domain
LRR_14 PF23598 588 - 643 8e-06 Leucine-rich repeat region
LRR_14 PF23598 650 - 944 3.8e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 890 - 1025 2e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 929
Acc16I TGCGCA 2 cut(s) 426, 2699
Acc36I ACCTGC 2 cut(s) 476, 1288
AccB1I GGYRCC 1 cut(s) 408
AccB7I CCANNNNNTGG 1 cut(s) 1474
AciI CCGC 5 cut(s) 885, 2659, 2684, 3270, 3334
AclI AACGTT 1 cut(s) 1709
AclWI GGATC 6 cut(s) 350, 363, 916, 1009, 2357, 2370
AcoI YGGCCR 2 cut(s) 1560, 1631
AcsI RAATTY 6 cut(s) 79, 1569, 1689, 2303, 2761, 2790
AcuI CTGAAG 4 cut(s) 1419, 1889, 1916, 2552
AcvI CACGTG 1 cut(s) 3214
AcyI GRCGYC 1 cut(s) 1452
AfaI GTAC 8 cut(s) 65, 988, 1484, 2081, 2468, 2477, 2807, 3218
AfiI CCNNNNNNNGG 4 cut(s) 405, 1474, 2737, 2954
AflII CTTAAG 1 cut(s) 2200
AflIII ACRYGT 4 cut(s) 736, 2524, 3211, 3213
AhlI ACTAGT 2 cut(s) 1790, 3055
AjnI CCWGG 5 cut(s) 227, 1836, 2686, 2730, 3105
AloI GAACNNNNNNTCC 2 cut(s) 2580, 2612
Alw21I GWGCWC 6 cut(s) 326, 748, 2749, 3077, 3200, 3225
Alw26I GTCTC 7 cut(s) 494, 860, 1190, 1781, 2305, 2697, 3042
Alw44I GTGCAC 3 cut(s) 744, 2745, 3221
AlwI GGATC 6 cut(s) 350, 363, 916, 1009, 2357, 2370
AoxI GGCC 5 cut(s) 3, 1560, 1588, 1631, 2653
ApaLI GTGCAC 3 cut(s) 744, 2745, 3221
ApeKI GCWGC 4 cut(s) 12, 1253, 2127, 2994
ApoI RAATTY 6 cut(s) 79, 1569, 1689, 2303, 2761, 2790
ArsI GACNNNNNNTTYG 4 cut(s) 943, 975, 1780, 1812
AseI ATTAAT 2 cut(s) 1641, 1938
Asp700I GAANNNNTTC 2 cut(s) 2034, 3354
AspLEI GCGC 3 cut(s) 427, 1005, 2700
AspS9I GGNCC 4 cut(s) 1108, 1589, 2647, 3280
AsuC2I CCSGG 6 cut(s) 86, 128, 754, 1190, 1524, 3278
AsuHPI GGTGA 7 cut(s) 101, 243, 742, 1048, 1128, 1685, 2452
AsuNHI GCTAGC 1 cut(s) 2168
AvaII GGWCC 3 cut(s) 1108, 2647, 3280
BaeGI GKGCMC 4 cut(s) 748, 1219, 2749, 3225
BalI TGGCCA 2 cut(s) 1562, 1633
BamHI GGATCC 2 cut(s) 355, 2362
BanI GGYRCC 1 cut(s) 408
BanII GRGCYC 1 cut(s) 664
BbrPI CACGTG 1 cut(s) 3214
BbsI GAAGAC 4 cut(s) 956, 1428, 2953, 3146
Bbv12I GWGCWC 6 cut(s) 326, 748, 2749, 3077, 3200, 3225
BbvI GCAGC 4 cut(s) 24, 1265, 2139, 2981
BccI CCATC 6 cut(s) 267, 1580, 1649, 2047, 2850, 3179
BciT130I CCWGG 5 cut(s) 229, 1838, 2688, 2732, 3107
BciVI GTATCC 1 cut(s) 805
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 6 cut(s) 86, 128, 754, 1190, 1524, 3278
BcoDI GTCTC 7 cut(s) 494, 860, 1190, 1781, 2305, 2697, 3042
BcuI ACTAGT 2 cut(s) 1790, 3055
BfaI CTAG 9 cut(s) 270, 405, 888, 966, 1497, 1791, 2169, 2639, 3056
BfmI CTRYAG 3 cut(s) 954, 1164, 2544
BfrI CTTAAG 1 cut(s) 2200
BfuAI ACCTGC 2 cut(s) 476, 1288
BfuI GTATCC 1 cut(s) 805
BglII AGATCT 1 cut(s) 1777
BisI GCNGC 5 cut(s) 13, 885, 1254, 2128, 2995
BlpI GCTNAGC 1 cut(s) 176
BlsI GCNGC 5 cut(s) 14, 886, 1255, 2129, 2996
Bme18I GGWCC 3 cut(s) 1108, 2647, 3280
BmgT120I GGNCC 4 cut(s) 1108, 1589, 2647, 3280
BmiI GGNNCC 8 cut(s) 357, 410, 1061, 1109, 2364, 2648, 3275, 3281
BmrI ACTGGG 1 cut(s) 2113
BmsI GCATC 7 cut(s) 32, 1090, 1423, 1527, 2008, 2448, 2806
BmtI GCTAGC 1 cut(s) 2172
BmuI ACTGGG 1 cut(s) 2113
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 4 cut(s) 956, 1428, 2953, 3146
BplI GAGNNNNNCTC 2 cut(s) 3059, 3091
BpmI CTGGAG 2 cut(s) 1820, 2483
Bpu1102I GCTNAGC 1 cut(s) 176
BpuMI CCSGG 6 cut(s) 86, 128, 754, 1190, 1524, 3278
Bsa29I ATCGAT 1 cut(s) 3170
BsaAI YACGTR 2 cut(s) 3214, 3220
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 1452
BsaI GGTCTC 3 cut(s) 494, 1190, 2697
BsaWI WCCGGW 1 cut(s) 1943
Bsc4I CCNNNNNNNGG 4 cut(s) 405, 1474, 2737, 2954
Bse1I ACTGG 3 cut(s) 457, 837, 2108
Bse3DI GCAATG 2 cut(s) 1359, 1964
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 5 cut(s) 229, 1838, 2688, 2732, 3107
BseCI ATCGAT 1 cut(s) 3170
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 4 cut(s) 405, 1474, 2737, 2954
BseMI GCAATG 2 cut(s) 1359, 1964
BseMII CTCAG 6 cut(s) 1795, 2598, 2667, 3047, 3280, 3374
BseNI ACTGG 3 cut(s) 457, 837, 2108
BseRI GAGGAG 2 cut(s) 42, 878
BseSI GKGCMC 4 cut(s) 748, 1219, 2749, 3225
BseXI GCAGC 4 cut(s) 24, 1265, 2139, 2981
BsgI GTGCAG 1 cut(s) 580
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 5 cut(s) 5, 1562, 1590, 1633, 2655
BshNI GGYRCC 1 cut(s) 408
BshVI ATCGAT 1 cut(s) 3170
BsiEI CGRYCG 1 cut(s) 240
BsiHKAI GWGCWC 6 cut(s) 326, 748, 2749, 3077, 3200, 3225
BsiSI CCGG 7 cut(s) 85, 127, 754, 1190, 1524, 1944, 3277
BslFI GGGAC 4 cut(s) 98, 1121, 1740, 3216
BslI CCNNNNNNNGG 4 cut(s) 405, 1474, 2737, 2954
BsmAI GTCTC 7 cut(s) 494, 860, 1190, 1781, 2305, 2697, 3042
BsmFI GGGAC 4 cut(s) 98, 1121, 1740, 3216
BsmI GAATGC 4 cut(s) 1071, 1862, 2170, 2240
BsnI GGCC 5 cut(s) 5, 1562, 1590, 1633, 2655
Bso31I GGTCTC 3 cut(s) 494, 1190, 2697
Bsp1286I GDGCHC 8 cut(s) 326, 664, 748, 1219, 2749, 3077, 3200, 3225
Bsp1407I TGTACA 1 cut(s) 2079
Bsp1720I GCTNAGC 1 cut(s) 176
Bsp19I CCATGG 4 cut(s) 642, 1053, 2650, 2938
BspACI CCGC 5 cut(s) 885, 2659, 2684, 3270, 3334
BspANI GGCC 5 cut(s) 5, 1562, 1590, 1633, 2655
BspCNI CTCAG 6 cut(s) 1794, 2597, 2668, 3046, 3279, 3373
BspDI ATCGAT 1 cut(s) 3170
BspLI GGNNCC 8 cut(s) 357, 410, 1061, 1109, 2364, 2648, 3275, 3281
BspMI ACCTGC 2 cut(s) 476, 1288
BspOI GCTAGC 1 cut(s) 2172
BspPI GGATC 6 cut(s) 350, 363, 916, 1009, 2357, 2370
BspQI GCTCTTC 3 cut(s) 669, 874, 3205
BspT107I GGYRCC 1 cut(s) 408
BspTI CTTAAG 1 cut(s) 2200
BspTNI GGTCTC 3 cut(s) 494, 1190, 2697
BsrDI GCAATG 2 cut(s) 1359, 1964
BsrGI TGTACA 1 cut(s) 2079
BsrI ACTGG 3 cut(s) 457, 837, 2108
BssNI GRCGYC 1 cut(s) 1452
BssT1I CCWWGG 5 cut(s) 642, 1053, 2512, 2650, 2938
Bst2UI CCWGG 5 cut(s) 229, 1838, 2688, 2732, 3107
Bst6I CTCTTC 6 cut(s) 669, 874, 2622, 3045, 3205, 3345
BstACI GRCGYC 1 cut(s) 1452
BstAFI CTTAAG 1 cut(s) 2200
BstAUI TGTACA 1 cut(s) 2079
BstBAI YACGTR 2 cut(s) 3214, 3220
BstC8I GCNNGC 7 cut(s) 423, 1069, 1502, 1635, 2170, 2240, 2657
BstDEI CTNAG 9 cut(s) 176, 1774, 1781, 1913, 2584, 2676, 3033, 3266, 3360
BstDSI CCRYGG 5 cut(s) 642, 1053, 1592, 2650, 2938
BstENI CCTNNNNNAGG 1 cut(s) 403
BstHHI GCGC 3 cut(s) 427, 1005, 2700
BstMAI GTCTC 7 cut(s) 494, 860, 1190, 1781, 2305, 2697, 3042
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 8 cut(s) 626, 1000, 1068, 1202, 1280, 2165, 2196, 2661
BstNI CCWGG 5 cut(s) 229, 1838, 2688, 2732, 3107
BstNSI RCATGY 3 cut(s) 740, 2025, 2528
BstPAI GACNNNNGTC 1 cut(s) 110
BstSFI CTRYAG 3 cut(s) 954, 1164, 2544
BstSLI GKGCMC 4 cut(s) 748, 1219, 2749, 3225
BstV1I GCAGC 4 cut(s) 24, 1265, 2139, 2981
BstV2I GAAGAC 4 cut(s) 956, 1428, 2953, 3146
BstX2I RGATCY 5 cut(s) 355, 921, 1014, 1777, 2362
BstYI RGATCY 5 cut(s) 355, 921, 1014, 1777, 2362
Bsu15I ATCGAT 1 cut(s) 3170
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 5 cut(s) 5, 1562, 1590, 1633, 2655
BsuTUI ATCGAT 1 cut(s) 3170
BtgI CCRYGG 5 cut(s) 642, 1053, 1592, 2650, 2938
BtsI GCAGTG 2 cut(s) 681, 2154
BtsIMutI CAGTG 7 cut(s) 311, 464, 681, 830, 1519, 2154, 2485
BveI ACCTGC 2 cut(s) 476, 1288
Cac8I GCNNGC 7 cut(s) 423, 1069, 1502, 1635, 2170, 2240, 2657
CfoI GCGC 3 cut(s) 427, 1005, 2700
Cfr13I GGNCC 4 cut(s) 1108, 1589, 2647, 3280
ClaI ATCGAT 1 cut(s) 3170
CseI GACGC 3 cut(s) 1460, 1992, 2337
Csp6I GTAC 8 cut(s) 64, 987, 1483, 2080, 2467, 2476, 2806, 3217
CspCI CAANNNNNGTGG 2 cut(s) 1581, 1616
CviQI GTAC 8 cut(s) 64, 987, 1483, 2080, 2467, 2476, 2806, 3217
DdeI CTNAG 9 cut(s) 176, 1774, 1781, 1913, 2584, 2676, 3033, 3266, 3360
DraI TTTAAA 1 cut(s) 3147
EaeI YGGCCR 2 cut(s) 1560, 1631
Eam1104I CTCTTC 6 cut(s) 669, 874, 2622, 3045, 3205, 3345
EarI CTCTTC 6 cut(s) 669, 874, 2622, 3045, 3205, 3345
Eco130I CCWWGG 5 cut(s) 642, 1053, 2512, 2650, 2938
Eco24I GRGCYC 1 cut(s) 664
Eco31I GGTCTC 3 cut(s) 494, 1190, 2697
Eco32I GATATC 2 cut(s) 1411, 2052
Eco47I GGWCC 3 cut(s) 1108, 2647, 3280
Eco57I CTGAAG 4 cut(s) 1419, 1889, 1916, 2552
Eco72I CACGTG 1 cut(s) 3214
EcoNI CCTNNNNNAGG 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRII CCWGG 5 cut(s) 227, 1836, 2686, 2730, 3105
EcoRV GATATC 2 cut(s) 1411, 2052
EcoT14I CCWWGG 5 cut(s) 642, 1053, 2512, 2650, 2938
EcoT22I ATGCAT 2 cut(s) 1542, 2023
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 5 cut(s) 642, 1053, 2512, 2650, 2938
FalI AAGNNNNNCTT 4 cut(s) 405, 437, 1358, 1390
FaqI GGGAC 4 cut(s) 98, 1121, 1740, 3216
FauI CCCGC 1 cut(s) 3263
FauNDI CATATG 1 cut(s) 2933
FbaI TGATCA 1 cut(s) 297
Fnu4HI GCNGC 5 cut(s) 13, 885, 1254, 2128, 2995
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 5 cut(s) 13, 885, 1254, 2128, 2995
FspBI CTAG 9 cut(s) 270, 405, 888, 966, 1497, 1791, 2169, 2639, 3056
FspI TGCGCA 2 cut(s) 426, 2699
GlaI GCGC 3 cut(s) 426, 1004, 2699
GluI GCNGC 5 cut(s) 13, 885, 1254, 2128, 2995
GsuI CTGGAG 2 cut(s) 1820, 2483
HaeIII GGCC 5 cut(s) 5, 1562, 1590, 1633, 2655
HapII CCGG 7 cut(s) 85, 127, 754, 1190, 1524, 1944, 3277
HgaI GACGC 3 cut(s) 1460, 1992, 2337
HhaI GCGC 3 cut(s) 427, 1005, 2700
Hin1I GRCGYC 1 cut(s) 1452
Hin6I GCGC 3 cut(s) 425, 1003, 2698
HinP1I GCGC 3 cut(s) 425, 1003, 2698
HincII GTYRAC 1 cut(s) 2184
HindII GTYRAC 1 cut(s) 2184
HindIII AAGCTT 3 cut(s) 1491, 1994, 2188
HpaI GTTAAC 1 cut(s) 2184
HpaII CCGG 7 cut(s) 85, 127, 754, 1190, 1524, 1944, 3277
HphI GGTGA 7 cut(s) 101, 243, 742, 1048, 1128, 1685, 2452
Hpy166II GTNNAC 9 cut(s) 746, 2184, 2218, 2417, 2747, 2808, 2920, 3217, 3223
Hpy188III TCNNGA 9 cut(s) 270, 668, 1531, 1553, 2338, 2586, 2639, 2786, 3347
Hpy8I GTNNAC 9 cut(s) 746, 2184, 2218, 2417, 2747, 2808, 2920, 3217, 3223
HpyCH4IV ACGT 5 cut(s) 1152, 1709, 2229, 3213, 3219
HpyF10VI GCNNNNNNNGC 8 cut(s) 626, 1000, 1068, 1202, 1280, 2165, 2196, 2661
HpyF3I CTNAG 9 cut(s) 176, 1774, 1781, 1913, 2584, 2676, 3033, 3266, 3360
HpySE526I ACGT 5 cut(s) 1152, 1709, 2229, 3213, 3219
Hsp92I GRCGYC 1 cut(s) 1452
HspAI GCGC 3 cut(s) 425, 1003, 2698
Ksp22I TGATCA 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 2184
LguI GCTCTTC 3 cut(s) 669, 874, 3205
LmnI GCTCC 3 cut(s) 55, 202, 1059
Lsp1109I GCAGC 4 cut(s) 24, 1265, 2139, 2981
LweI GCATC 7 cut(s) 32, 1090, 1423, 1527, 2008, 2448, 2806
MaeI CTAG 9 cut(s) 270, 405, 888, 966, 1497, 1791, 2169, 2639, 3056
MaeII ACGT 5 cut(s) 1152, 1709, 2229, 3213, 3219
MaeIII GTNAC 6 cut(s) 2011, 2090, 2389, 2440, 2679, 3085
MfeI CAATTG 2 cut(s) 2266, 3047
MflI RGATCY 5 cut(s) 355, 921, 1014, 1777, 2362
MhlI GDGCHC 8 cut(s) 326, 664, 748, 1219, 2749, 3077, 3200, 3225
MlsI TGGCCA 2 cut(s) 1562, 1633
MluNI TGGCCA 2 cut(s) 1562, 1633
MlyI GAGTC 2 cut(s) 2386, 2967
MmeI TCCRAC 3 cut(s) 1410, 1986, 2340
Mox20I TGGCCA 2 cut(s) 1562, 1633
Mph1103I ATGCAT 2 cut(s) 1542, 2023
MroXI GAANNNNTTC 2 cut(s) 2034, 3354
MscI TGGCCA 2 cut(s) 1562, 1633
MslI CAYNNNNRTG 2 cut(s) 741, 2498
Msp20I TGGCCA 2 cut(s) 1562, 1633
MspA1I CMGCKG 1 cut(s) 3270
MspCI CTTAAG 1 cut(s) 2200
MspI CCGG 7 cut(s) 85, 127, 754, 1190, 1524, 1944, 3277
MunI CAATTG 2 cut(s) 2266, 3047
Mva1269I GAATGC 4 cut(s) 1071, 1862, 2170, 2240
MvaI CCWGG 5 cut(s) 229, 1838, 2688, 2732, 3107
MwoI GCNNNNNNNGC 8 cut(s) 626, 1000, 1068, 1202, 1280, 2165, 2196, 2661
NciI CCSGG 6 cut(s) 86, 128, 754, 1190, 1524, 3278
NcoI CCATGG 4 cut(s) 642, 1053, 2650, 2938
NdeI CATATG 1 cut(s) 2933
NheI GCTAGC 1 cut(s) 2168
NlaIV GGNNCC 8 cut(s) 357, 410, 1061, 1109, 2364, 2648, 3275, 3281
NmuCI GTSAC 3 cut(s) 2090, 2389, 2440
NsbI TGCGCA 2 cut(s) 426, 2699
NsiI ATGCAT 2 cut(s) 1542, 2023
NspI RCATGY 3 cut(s) 740, 2025, 2528
PciI ACATGT 2 cut(s) 736, 2524
PciSI GCTCTTC 3 cut(s) 669, 874, 3205
PctI GAATGC 4 cut(s) 1071, 1862, 2170, 2240
PdmI GAANNNNTTC 2 cut(s) 2034, 3354
PfeI GAWTC 8 cut(s) 224, 503, 1133, 1527, 1732, 1909, 2256, 2837
PflMI CCANNNNNTGG 1 cut(s) 1474
PkrI GCNGC 5 cut(s) 14, 886, 1255, 2129, 2996
Ple19I CGATCG 1 cut(s) 240
PleI GAGTC 2 cut(s) 2386, 2966
PmaCI CACGTG 1 cut(s) 3214
PmlI CACGTG 1 cut(s) 3214
PpsI GAGTC 2 cut(s) 2386, 2966
Ppu21I YACGTR 2 cut(s) 3214, 3220
PpuMI RGGWCCY 1 cut(s) 1108
PscI ACATGT 2 cut(s) 736, 2524
PshAI GACNNNNGTC 1 cut(s) 110
PshBI ATTAAT 2 cut(s) 1641, 1938
PsiI TTATAA 1 cut(s) 929
Psp1406I AACGTT 1 cut(s) 1709
Psp5II RGGWCCY 1 cut(s) 1108
Psp6I CCWGG 5 cut(s) 227, 1836, 2686, 2730, 3105
PspCI CACGTG 1 cut(s) 3214
PspGI CCWGG 5 cut(s) 227, 1836, 2686, 2730, 3105
PspN4I GGNNCC 8 cut(s) 357, 410, 1061, 1109, 2364, 2648, 3275, 3281
PspPI GGNCC 4 cut(s) 1108, 1589, 2647, 3280
PspPPI RGGWCCY 1 cut(s) 1108
PsuI RGATCY 5 cut(s) 355, 921, 1014, 1777, 2362
PvuI CGATCG 1 cut(s) 240
RsaI GTAC 8 cut(s) 65, 988, 1484, 2081, 2468, 2477, 2807, 3218
RsaNI GTAC 8 cut(s) 64, 987, 1483, 2080, 2467, 2476, 2806, 3217
RseI CAYNNNNRTG 2 cut(s) 741, 2498
SapI GCTCTTC 3 cut(s) 669, 874, 3205
SatI GCNGC 5 cut(s) 13, 885, 1254, 2128, 2995
Sau96I GGNCC 4 cut(s) 1108, 1589, 2647, 3280
SchI GAGTC 2 cut(s) 2386, 2967
SduI GDGCHC 8 cut(s) 326, 664, 748, 1219, 2749, 3077, 3200, 3225
SfaNI GCATC 7 cut(s) 32, 1090, 1423, 1527, 2008, 2448, 2806
SfcI CTRYAG 3 cut(s) 954, 1164, 2544
SinI GGWCC 3 cut(s) 1108, 2647, 3280
SmiI ATTTAAAT 1 cut(s) 3147
SmiMI CAYNNNNRTG 2 cut(s) 741, 2498
SmlI CTYRAG 2 cut(s) 7, 2200
SmoI CTYRAG 2 cut(s) 7, 2200
SpeI ACTAGT 2 cut(s) 1790, 3055
SsiI CCGC 5 cut(s) 885, 2659, 2684, 3270, 3334
SspI AATATT 2 cut(s) 820, 1645
SspMI CTAG 9 cut(s) 270, 405, 888, 966, 1497, 1791, 2169, 2639, 3056
StyI CCWWGG 5 cut(s) 642, 1053, 2512, 2650, 2938
SwaI ATTTAAAT 1 cut(s) 3147
TaiI ACGT 5 cut(s) 1155, 1712, 2232, 3216, 3222
TaqI TCGA 9 cut(s) 1258, 1413, 1419, 1552, 1580, 2337, 2433, 3018, 3170
TatI WGTACW 3 cut(s) 2079, 2466, 2805
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 8 cut(s) 224, 503, 1133, 1527, 1732, 1909, 2256, 2837
TscAI CASTG 7 cut(s) 311, 464, 688, 837, 1519, 2161, 2485
TseFI GTSAC 3 cut(s) 2090, 2389, 2440
TseI GCWGC 4 cut(s) 12, 1253, 2127, 2994
Tsp45I GTSAC 3 cut(s) 2090, 2389, 2440
TspGWI ACGGA 5 cut(s) 2030, 2340, 2423, 2825, 2954
TspRI CASTG 7 cut(s) 311, 464, 688, 837, 1519, 2161, 2485
Van91I CCANNNNNTGG 1 cut(s) 1474
Vha464I CTTAAG 1 cut(s) 2200
VneI GTGCAC 3 cut(s) 744, 2745, 3221
VpaK11BI GGWCC 3 cut(s) 1108, 2647, 3280
VspI ATTAAT 2 cut(s) 1641, 1938
XagI CCTNNNNNAGG 1 cut(s) 403
XapI RAATTY 6 cut(s) 79, 1569, 1689, 2303, 2761, 2790
XbaI TCTAGA 2 cut(s) 269, 2638
XceI RCATGY 3 cut(s) 740, 2025, 2528
XcmI CCANNNNNNNNNTGG 1 cut(s) 2564
XmnI GAANNNNTTC 2 cut(s) 2034, 3354
XspI CTAG 9 cut(s) 270, 405, 888, 966, 1497, 1791, 2169, 2639, 3056
Zsp2I ATGCAT 2 cut(s) 1542, 2023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.