FvH4_5g01271

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000085
Physical Location & Seq
Forward (+)
785317 .. 790315
4999 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g01271.t1

Sequence Viewer

Length: 4041 bp
ATGGAAGGTACCCGAGATACTGAAATAGCAATGGGGGCTTATCAGCCTCATCATACTTGGGCAAGAAAACTCTCAAGTCCACATGGAAAGGTATATGGATATAGAATGTCACTGTGGGCAGAGCATATAGGACTTGTTGAAAAATGTTTCAAGCAGCCAGAGAGTCTTGAATGTGTCCAAAGAGTAAGGACTTTGAGTGAGCAGAACTGGAGTCAATACATAGCCGATGAAGTCACAGACATGAAGGGTCACCTCCTGAAATATCCGGTTGAAGTTAGTCCAACCGGCAAGGTGGAGCCTCTTCCGGGATGCGCAGCATTTCCAGATGTTGGCGGCTACATATTGGGGAGTTCTGACGTTCAGTTTACAGAGTTGACAGGAGATTTTGAAACAACAGGAGATGTTCGGTCCATGGCTTCTCCCCAGCCTGAGCCAGCTCCTGTGAAGAATGATGTGCTTTCAGGTTTCGGGATGGAAGACGCTCGCAGGGGTATTGTAGCCCATTCAGACCATGAATTGGAAAGCACAGGAATAAGTAGAACATTCAGGGATGAAGACCACTTTGAAAAAGGGGTAGCCCTCCCATCTACATCACCAGTTATTGGGCAAAAAAATGATGAGTTTTTGAATACCAGGGGAGAGGACAATCATAGGGGTTTTGTCACCCATTTGGGTCACGAAACACAAAACACCGATGAAAATCATCTTGAAGAAGAGATACCTGTCCCCTCTACGTCTCCACCTTCACCAGCTGTGGGAGATCATCGGGTGAAGTATGATGTGTTTTTGAGTTTCAGGGGGGAAGATACTCGAAAGGGTTTTATCGCCTATTTATACCGTGAATTGCAAAAAACCAGAGCGATTAAAACTTTCAAGGATGACGCGCAACTGAAAAAAGGGACTGTTATTGCTTCAAATCTCCTAAGGGCAATCGAAGAATCAAGGTTTGCGATTATTGTGTTTTCCGAGAAGTATGCTGATTCTGCATGGTGTTTGGATGAACTTACAAAGATTTTTCAATGCATGGAGGGTAAGGACACAATTCTCCCAATTTTTTATCAGTTGGATCCCGCTGTTGTACGATATCAAAAGAAAGAGACCAGTTTTGGTGAAGCTTTCGTCAAGCATGAAGCTAGACAAGACATTGAGAAGACGAACCAGTGGAGAGCTGCATTAAAAGGAGTGGCAGGAATCTCTGGGTGGAATTCAAACACTTTTCCGACGGAGGTAGAACTAATCGATGACATTGTGAAACATTTGTTGGAAAAAGTACTTCAAGCACCCACCAGAACTTTTGGAACATTTGAAGCGACAGGGCAAGTCATGGATGAGGTCATGAAGGTGTTAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTAGCTGGCGTTGGAAAGACGACTATGGTCAAACATGTTGGTGCACAAGCTGAGAAAGATGGGCTTTTTGATCGTGTGATTATGGTTGTCATATCCAAAAGCCCTGACTTGCAAGAAATTCAAGGCACGCTAGCAGATTCATTGGGCCTGAAGGAGGGGATCAAGAGAGGAGATAGGATGCTTATAATCTTGGACGACATTTGGGAGAGTTTTGAACTCTCAGGCATTGGAATTCCCGGTCACCATGAGCTTCAAAACTGTTACTCTAAAGTTGTAATCATCACAAGGAACAGCAACGTCTGCCATTCTATGGGGTGTCATGCCCAGATCGCTCTCAATATTCTATCAGATGAAGATTCTTGGAAGTTACTCAAAGAGACCTCGGGGAAGTCTTTTGATGAATCTGCAAATTCCTATGATGAAGAGAGGAAGGTAGCTCAAGAATGCAGAGGTCTCCCAAGAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACCGGGATGAATGGAATGAAGCAGTTGCAAGGGAATTTGGAGGCTTGGACGAATGGAATGAAGCAGCTCAAGCCTTCAACCTTGAAGCTGAGAAGGGCTCTTTTCATTCATTGGAGGATACATATCACTCACTGGAATCTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCTTATTCCCAGAAAATTATGATATCCCAATTGAGAAGTTGCTCAAGTATGGGATTGGAATAGGTTTGTTTCAATATGATTCAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTTAAGTACCTCAAAGCTTCTAGATTACTTCTGGAGGGTACAAAGGAGGGATGTGTAAGGACGACCGATGACATTCGAAATCTGGCATTGTCCGAGGAAGGCGATGGGCTTTTAGTAAAAGCTGGTTGTGAGCTAGAGGAGTGGCCAGACGCAGAGGCAGTATGGTCGCAAGGATTGACTTGGACCCATTCCTGGCACTGGGATTGTCCAATTGATACTCATGAAGGCTACTCAATAATCTCACTAATGAGAAACAAGCTTCACAAGCTTCCTGAATATTTGGTATGTTCAAAGCTCCAAATTTTGTTACTACAAAGTAATGCTGGTATATGTGACATTCCAGAAACCTTTCTTCAAAGTCCTAGTGCGCTAAGGGTCTTTGATCTTAGCTTCACCAGTATTTCGTCACTACCTCAGTCATTCAGTTTCCTAACCAGCCTCCAAGCTTTGAATTTGGATTTTTGCAGCAATTTAAGTGAGATATCTGTCTTAGGAAATCTTAAGAAACTTGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAATTTACCAAAACAAATAGGAAATTTGACCAGTCTGAGGAAGTTGGACGTCACTGGTAGATATCTTGAAACAATCCCATCTAAAGTGTTATCGAAGTTGTGTAGATTGGAAGAACTGCACATGAAGTTTCAATTTGGGAAGTGGGGAAGTAAAATTGAGGATGCATCTGAAGAAACCAACGCTAACTTTGATGAGTTGACTAGTTTGCTGGATTTAAACATTTTGGAAGTTTACATATCTGATGCTACATGCTTGCCTCAAAGTGTTGAGTACTTCAACCCGAATTGGGTAGAATTTGATATATGCATCAGCAGGGACGTGTTGCTCCCTCGATATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAATAGCTTACCGGATTGGTTTATCAGCGTTGTGACAGCGAAAGTGGAGCGGCTAGAGTACACAAATTGCGATGATTTGAATGACCTTGTTGGGGAATATGACCATGGCAGATTATTTGTGCTGAAATATCTCTCTGTGAACGGGCACCATAAGAACTTGAAAGAGATAATCAACACAATGAGACGGGTTTCAAATAAACCGGTATTTGAGAACTTGGAAGATTTGCATCTGCACTGCTTATATTCCCTCGAGGAGTTGTGTGTTGGTGAGCTACCACCTGGGTCCCTACGCAAGCTCAAGTTATTGAAAGTGCAAACATGTCATAGCTTGGTGAATGCACTTTTACCATTGACATTGTTGCCGAGATTACAAAATCTGGAAAAAATCATTTGTGTACATCTGGACAGAATGAAATATGTTTTTGGATCTGATGAAGGGGGTGTTGATTCAGAGAAGATTTTCTTGAGGAAATTGAGGGAGATGAGATTGTGTGCTCTAGAAGAACTAGTAAGAATATGCAACGGTCCTGCTCCGCATGCAATCTTCCAGAATCTAAAAGTTTTGGCAATCTGCGAGTGCAAGGAACTGAAAAGCCTCTTCACAATAGACGTTGCTAAATGTCTTTTTCAGTTGGAAGACCTTTGCATAGAGCAGTGTCCTCGCTTGGACAGAGTCATTGAAGCAAGCAATAGCTTGAACAGCGAGATTACACTTCCGGCGTTGAAGAGATTAGCTTTGATTGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAAGTGGTAATATTGAAACTCGGTGTCGGTGCCCTTCATTGGAATACTTGTACGTGGAGAACTGCATCAAGTTTTCATATTATGCAAACTTCCACTTCAGTAAGAAGCAAATTCACTTCAATGATCAACACCATTTGAGTTCTCTAGTGAAAAGGTGGAAGGAAATGCATAATCAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1347

Amino Acids

152.23

Weight (kDa)

5.43

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLD_C PF12357 47 - 117 7.3e-30 Phospholipase D C terminal
TIR PF01582 258 - 430 2.3e-47 TIR domain
TIR_2 PF13676 261 - 357 4.3e-13 TIR domain
NB-ARC PF00931 441 - 590 1.8e-23 NB-ARC domain
LRR_14 PF23598 805 - 993 6.7e-12 Leucine-rich repeat region
LRR_8 PF13855 821 - 878 7.9e-08 Leucine rich repeat
LRR_RPS2 PF23247 1101 - 1248 1.2e-23 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1568
AatII GACGTC 1 cut(s) 2763
AbsI CCTCGAGG 1 cut(s) 3335
Acc16I TGCGCA 1 cut(s) 313
Acc65I GGTACC 1 cut(s) 8
AccB1I GGYRCC 3 cut(s) 8, 3231, 3888
AccB7I CCANNNNNTGG 4 cut(s) 329, 517, 602, 1693
AccBSI CCGCTC 1 cut(s) 3136
AccII CGCG 1 cut(s) 884
AciI CCGC 4 cut(s) 333, 1071, 3136, 3620
AclWI GGATC 4 cut(s) 1061, 1074, 1550, 3520
AcoI YGGCCR 1 cut(s) 2312
AcuI CTGAAG 3 cut(s) 1553, 2901, 3940
AcyI GRCGYC 1 cut(s) 2760
AfaI GTAC 9 cut(s) 10, 1080, 1272, 2177, 2209, 2984, 3146, 3483, 3911
AflII CTTAAG 2 cut(s) 2669, 2708
AflIII ACRYGT 3 cut(s) 1417, 3030, 3404
AgeI ACCGGT 1 cut(s) 3286
AhlI ACTAGT 2 cut(s) 2912, 3592
AjiI CACGTC 1 cut(s) 3031
AjnI CCWGG 3 cut(s) 632, 2360, 3364
AjuI GAANNNNNNNTTGG 4 cut(s) 1244, 1276, 2024, 2056
Alw21I GWGCWC 4 cut(s) 1429, 2161, 3583, 3843
Alw26I GTCTC 5 cut(s) 741, 1091, 1754, 1841, 3262
Alw44I GTGCAC 1 cut(s) 1425
AlwI GGATC 4 cut(s) 1061, 1074, 1550, 3520
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 3 cut(s) 12, 1765, 3335
AoxI GGCC 2 cut(s) 1528, 2312
ApaLI GTGCAC 1 cut(s) 1425
ApeKI GCWGC 8 cut(s) 154, 314, 1169, 1856, 1859, 1946, 2052, 2634
AsiGI ACCGGT 1 cut(s) 3286
Asp700I GAANNNNTTC 2 cut(s) 2517, 3545
Asp718I GGTACC 1 cut(s) 8
AspLEI GCGC 3 cut(s) 314, 886, 2539
AspS9I GGNCC 5 cut(s) 408, 1528, 2352, 3369, 3611
AsuC2I CCSGG 3 cut(s) 306, 1620, 1886
AsuII TTCGAA 1 cut(s) 2245
AsuNHI GCTAGC 1 cut(s) 1513
AvaI CYCGRG 3 cut(s) 12, 1765, 3335
AvaII GGWCC 4 cut(s) 408, 2352, 3369, 3611
AxyI CCTNAGG 1 cut(s) 923
BaeGI GKGCMC 3 cut(s) 1429, 3234, 3893
BalI TGGCCA 1 cut(s) 2314
BamHI GGATCC 1 cut(s) 1066
BanI GGYRCC 3 cut(s) 8, 3231, 3888
BanII GRGCYC 1 cut(s) 1982
BarI GAAGNNNNNNTAC 2 cut(s) 702, 734
BbsI GAAGAC 4 cut(s) 483, 561, 1157, 3729
Bbv12I GWGCWC 4 cut(s) 1429, 2161, 3583, 3843
BbvI GCAGC 8 cut(s) 166, 326, 1156, 1846, 1868, 1958, 2039, 2646
BccI CCATC 6 cut(s) 466, 592, 1436, 2267, 2797, 3044
BcgI CGANNNNNNTGC 6 cut(s) 956, 990, 2316, 2350, 3728, 3762
BciT130I CCWGG 3 cut(s) 634, 2362, 3366
BciVI GTATCC 1 cut(s) 1993
BclI TGATCA 2 cut(s) 1354, 3982
BcnI CCSGG 3 cut(s) 306, 1620, 1886
BcoDI GTCTC 5 cut(s) 741, 1091, 1754, 1841, 3262
BcuI ACTAGT 2 cut(s) 2912, 3592
BfrI CTTAAG 2 cut(s) 2669, 2708
BfuI GTATCC 1 cut(s) 1993
BmcAI AGTACT 2 cut(s) 1272, 2984
Bme1390I CCNGG 6 cut(s) 306, 634, 1620, 1886, 2362, 3366
Bme18I GGWCC 4 cut(s) 408, 2352, 3369, 3611
BmeT110I CYCGRG 3 cut(s) 12, 1765, 3335
BmgBI CACGTC 1 cut(s) 3031
BmgT120I GGNCC 5 cut(s) 408, 1528, 2352, 3369, 3611
BmiI GGNNCC 8 cut(s) 10, 297, 1068, 2354, 3233, 3370, 3371, 3890
BmrFI CCNGG 6 cut(s) 306, 634, 1620, 1886, 2362, 3366
BmrI ACTGGG 1 cut(s) 2377
BmsI GCATC 9 cut(s) 299, 1551, 2017, 2863, 2885, 2944, 3027, 3322, 3933
BmtI GCTAGC 1 cut(s) 1517
BmuI ACTGGG 1 cut(s) 2377
BoxI GACNNNNGTC 1 cut(s) 1409
BpiI GAAGAC 4 cut(s) 483, 561, 1157, 3729
BplI GAGNNNNNCTC 2 cut(s) 1964, 1996
BpmI CTGGAG 2 cut(s) 229, 2222
Bpu10I CCTNAGC 2 cut(s) 429, 2540
Bpu14I TTCGAA 1 cut(s) 2245
BpuEI CTTGAG 7 cut(s) 58, 1806, 1935, 2081, 2699, 3368, 3571
BpuMI CCSGG 3 cut(s) 306, 1620, 1886
Bsa29I ATCGAT 1 cut(s) 1239
BsaAI YACGTR 1 cut(s) 3913
BsaBI GATNNNNATC 3 cut(s) 699, 2004, 3312
BsaHI GRCGYC 1 cut(s) 2760
BsaI GGTCTC 3 cut(s) 1091, 1754, 1841
BsaJI CCNNGG 7 cut(s) 411, 633, 1369, 1764, 2262, 3190, 3365
BsaWI WCCGGW 4 cut(s) 265, 3097, 3286, 3835
Bse118I RCCGGY 2 cut(s) 284, 3286
Bse1I ACTGG 9 cut(s) 212, 596, 1101, 1159, 2019, 2372, 2565, 2742, 2770
Bse21I CCTNAGG 1 cut(s) 923
Bse3DI GCAATG 1 cut(s) 36
Bse8I GATNNNNATC 3 cut(s) 699, 2004, 3312
BseBI CCWGG 3 cut(s) 634, 2362, 3366
BseCI ATCGAT 1 cut(s) 1239
BseDI CCNNGG 7 cut(s) 411, 633, 1369, 1764, 2262, 3190, 3365
BseJI GATNNNNATC 3 cut(s) 699, 2004, 3312
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 6 cut(s) 420, 1425, 1617, 1962, 2597, 2738
BseNI ACTGG 9 cut(s) 212, 596, 1101, 1159, 2019, 2372, 2565, 2742, 2770
BseRI GAGGAG 3 cut(s) 1566, 2321, 3353
BseSI GKGCMC 3 cut(s) 1429, 3234, 3893
BseXI GCAGC 8 cut(s) 166, 326, 1156, 1846, 1868, 1958, 2039, 2646
BseYI CCCAGC 1 cut(s) 423
BsgI GTGCAG 3 cut(s) 2813, 3302, 3838
Bsh1236I CGCG 1 cut(s) 884
Bsh1285I CGRYCG 1 cut(s) 2235
BshFI GGCC 2 cut(s) 1530, 2314
BshNI GGYRCC 3 cut(s) 8, 3231, 3888
BshTI ACCGGT 1 cut(s) 3286
BshVI ATCGAT 1 cut(s) 1239
BsiEI CGRYCG 1 cut(s) 2235
BsiHKAI GWGCWC 4 cut(s) 1429, 2161, 3583, 3843
BsiHKCI CYCGRG 3 cut(s) 12, 1765, 3335
BsiSI CCGG 9 cut(s) 266, 285, 305, 1620, 1885, 3098, 3287, 3803, 3836
BslFI GGGAC 4 cut(s) 710, 913, 3041, 3355
BsmAI GTCTC 5 cut(s) 741, 1091, 1754, 1841, 3262
BsmBI CGTCTC 2 cut(s) 741, 3262
BsmFI GGGAC 4 cut(s) 710, 913, 3041, 3355
BsmI GAATGC 2 cut(s) 1832, 3427
BsnI GGCC 2 cut(s) 1530, 2314
Bso31I GGTCTC 3 cut(s) 1091, 1754, 1841
BsoBI CYCGRG 3 cut(s) 12, 1765, 3335
Bsp119I TTCGAA 1 cut(s) 2245
Bsp1286I GDGCHC 7 cut(s) 1429, 1982, 2161, 3234, 3583, 3843, 3893
Bsp1407I TGTACA 1 cut(s) 3481
Bsp19I CCATGG 2 cut(s) 411, 3190
BspACI CCGC 4 cut(s) 333, 1071, 3136, 3620
BspANI GGCC 2 cut(s) 1530, 2314
BspCNI CTCAG 6 cut(s) 421, 1426, 1616, 1963, 2596, 2739
BspDI ATCGAT 1 cut(s) 1239
BspFNI CGCG 1 cut(s) 884
BspHI TCATGA 2 cut(s) 1335, 2389
BspLI GGNNCC 8 cut(s) 10, 297, 1068, 2354, 3233, 3370, 3371, 3890
BspOI GCTAGC 1 cut(s) 1517
BspPI GGATC 4 cut(s) 1061, 1074, 1550, 3520
BspT104I TTCGAA 1 cut(s) 2245
BspT107I GGYRCC 3 cut(s) 8, 3231, 3888
BspTI CTTAAG 2 cut(s) 2669, 2708
BspTNI GGTCTC 3 cut(s) 1091, 1754, 1841
BsrBI CCGCTC 1 cut(s) 3136
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 2 cut(s) 284, 3286
BsrGI TGTACA 1 cut(s) 3481
BsrI ACTGG 9 cut(s) 212, 596, 1101, 1159, 2019, 2372, 2565, 2742, 2770
BssAI RCCGGY 2 cut(s) 284, 3286
BssECI CCNNGG 7 cut(s) 411, 633, 1369, 1764, 2262, 3190, 3365
BssNI GRCGYC 1 cut(s) 2760
BssT1I CCWWGG 3 cut(s) 411, 1369, 3190
Bst2UI CCWGG 3 cut(s) 634, 2362, 3366
Bst4CI ACNGT 6 cut(s) 114, 839, 904, 1366, 1643, 3611
Bst6I CTCTTC 5 cut(s) 306, 708, 1800, 3689, 3806
BstACI GRCGYC 1 cut(s) 2760
BstAFI CTTAAG 2 cut(s) 2669, 2708
BstAPI GCANNNNNTGC 1 cut(s) 1683
BstAUI TGTACA 1 cut(s) 3481
BstBAI YACGTR 1 cut(s) 3913
BstBI TTCGAA 1 cut(s) 2245
BstC8I GCNNGC 9 cut(s) 435, 484, 1390, 1511, 1515, 2966, 3380, 3624, 3772
BstDSI CCRYGG 2 cut(s) 411, 3190
BstEII GGTNACC 2 cut(s) 248, 1622
BstENI CCTNNNNNAGG 1 cut(s) 1535
BstFNI CGCG 1 cut(s) 884
BstHHI GCGC 3 cut(s) 314, 886, 2539
BstMAI GTCTC 5 cut(s) 741, 1091, 1754, 1841, 3262
BstMCI CGRYCG 1 cut(s) 2235
BstNI CCWGG 3 cut(s) 634, 2362, 3366
BstNSI RCATGY 4 cut(s) 1421, 2964, 3408, 3626
BstPAI GACNNNNGTC 1 cut(s) 1409
BstPI GGTNACC 2 cut(s) 248, 1622
BstSCI CCNGG 6 cut(s) 304, 632, 1618, 1884, 2360, 3364
BstSLI GKGCMC 3 cut(s) 1429, 3234, 3893
BstUI CGCG 1 cut(s) 884
BstV1I GCAGC 8 cut(s) 166, 326, 1156, 1846, 1868, 1958, 2039, 2646
BstV2I GAAGAC 4 cut(s) 483, 561, 1157, 3729
BstX2I RGATCY 2 cut(s) 1066, 3512
BstYI RGATCY 2 cut(s) 1066, 3512
Bsu15I ATCGAT 1 cut(s) 1239
Bsu36I CCTNAGG 1 cut(s) 923
BsuI GTATCC 1 cut(s) 1993
BsuRI GGCC 2 cut(s) 1530, 2314
BsuTUI ATCGAT 1 cut(s) 1239
BtgI CCRYGG 2 cut(s) 411, 3190
BtgZI GCGATG 2 cut(s) 2286, 3171
BtrI CACGTC 1 cut(s) 3031
BtsI GCAGTG 2 cut(s) 3319, 3746
BtsIMutI CAGTG 9 cut(s) 110, 1166, 1362, 2012, 2365, 2763, 3319, 3746, 3855
Cac8I GCNNGC 9 cut(s) 435, 484, 1390, 1511, 1515, 2966, 3380, 3624, 3772
CaiI CAGNNNCTG 1 cut(s) 440
CciI TCATGA 2 cut(s) 1335, 2389
CfoI GCGC 3 cut(s) 314, 886, 2539
Cfr10I RCCGGY 2 cut(s) 284, 3286
Cfr13I GGNCC 5 cut(s) 408, 1528, 2352, 3369, 3611
ClaI ATCGAT 1 cut(s) 1239
CseI GACGC 3 cut(s) 488, 890, 2327
Csp6I GTAC 9 cut(s) 9, 1079, 1271, 2176, 2208, 2983, 3145, 3482, 3910
CspAI ACCGGT 1 cut(s) 3286
CviQI GTAC 9 cut(s) 9, 1079, 1271, 2176, 2208, 2983, 3145, 3482, 3910
DraI TTTAAA 1 cut(s) 2928
EaeI YGGCCR 1 cut(s) 2312
Eam1104I CTCTTC 5 cut(s) 306, 708, 1800, 3689, 3806
EarI CTCTTC 5 cut(s) 306, 708, 1800, 3689, 3806
Eco130I CCWWGG 3 cut(s) 411, 1369, 3190
Eco24I GRGCYC 1 cut(s) 1982
Eco31I GGTCTC 3 cut(s) 1091, 1754, 1841
Eco32I GATATC 4 cut(s) 1085, 2077, 2652, 2774
Eco47I GGWCC 4 cut(s) 408, 2352, 3369, 3611
Eco57I CTGAAG 3 cut(s) 1553, 2901, 3940
Eco81I CCTNAGG 1 cut(s) 923
Eco88I CYCGRG 3 cut(s) 12, 1765, 3335
Eco91I GGTNACC 2 cut(s) 248, 1622
EcoNI CCTNNNNNAGG 1 cut(s) 1535
EcoO109I RGGNCCY 1 cut(s) 3369
EcoO65I GGTNACC 2 cut(s) 248, 1622
EcoRI GAATTC 2 cut(s) 1204, 1614
EcoRII CCWGG 3 cut(s) 632, 2360, 3364
EcoRV GATATC 4 cut(s) 1085, 2077, 2652, 2774
EcoT14I CCWWGG 3 cut(s) 411, 1369, 3190
EcoT22I ATGCAT 4 cut(s) 1025, 2878, 3020, 4029
EcoT38I GRGCYC 1 cut(s) 1982
ErhI CCWWGG 3 cut(s) 411, 1369, 3190
Esp3I CGTCTC 2 cut(s) 741, 3262
FalI AAGNNNNNCTT 6 cut(s) 1431, 1463, 2024, 2056, 3533, 3565
FaqI GGGAC 4 cut(s) 710, 913, 3041, 3355
FauI CCCGC 1 cut(s) 1078
FbaI TGATCA 2 cut(s) 1354, 3982
FriOI GRGCYC 1 cut(s) 1982
FspI TGCGCA 1 cut(s) 313
GlaI GCGC 3 cut(s) 313, 885, 2538
GsaI CCCAGC 1 cut(s) 427
GsuI CTGGAG 2 cut(s) 229, 2222
HaeIII GGCC 2 cut(s) 1530, 2314
HapII CCGG 9 cut(s) 266, 285, 305, 1620, 1885, 3098, 3287, 3803, 3836
HgaI GACGC 3 cut(s) 488, 890, 2327
HhaI GCGC 3 cut(s) 314, 886, 2539
Hin1I GRCGYC 1 cut(s) 2760
Hin6I GCGC 3 cut(s) 312, 884, 2537
HinP1I GCGC 3 cut(s) 312, 884, 2537
HincII GTYRAC 2 cut(s) 375, 2910
HindII GTYRAC 2 cut(s) 375, 2910
HindIII AAGCTT 5 cut(s) 1113, 2184, 2426, 2435, 2613
HpaII CCGG 9 cut(s) 266, 285, 305, 1620, 1885, 3098, 3287, 3803, 3836
Hpy166II GTNNAC 9 cut(s) 80, 366, 375, 1427, 2910, 2944, 3147, 3226, 3482
Hpy8I GTNNAC 9 cut(s) 80, 366, 375, 1427, 2910, 2944, 3147, 3226, 3482
Hpy99I CGWCG 1 cut(s) 1225
HpyCH4III ACNGT 6 cut(s) 114, 839, 904, 1366, 1643, 3611
HpyCH4IV ACGT 7 cut(s) 357, 734, 1680, 2760, 3030, 3696, 3912
HpySE526I ACGT 7 cut(s) 357, 734, 1680, 2760, 3030, 3696, 3912
Hsp92I GRCGYC 1 cut(s) 2760
HspAI GCGC 3 cut(s) 312, 884, 2537
KflI GGGWCCC 1 cut(s) 3369
KpnI GGTACC 1 cut(s) 12
Ksp22I TGATCA 2 cut(s) 1354, 3982
LmnI GCTCC 6 cut(s) 295, 442, 2469, 3042, 3133, 3622
Lsp1109I GCAGC 8 cut(s) 166, 326, 1156, 1846, 1868, 1958, 2039, 2646
LweI GCATC 9 cut(s) 299, 1551, 2017, 2863, 2885, 2944, 3027, 3322, 3933
MaeII ACGT 7 cut(s) 357, 734, 1680, 2760, 3030, 3696, 3912
MbiI CCGCTC 1 cut(s) 3136
MfeI CAATTG 2 cut(s) 2082, 2379
MflI RGATCY 2 cut(s) 1066, 3512
MhlI GDGCHC 7 cut(s) 1429, 1982, 2161, 3234, 3583, 3843, 3893
MlsI TGGCCA 1 cut(s) 2314
MluNI TGGCCA 1 cut(s) 2314
MlyI GAGTC 4 cut(s) 172, 220, 3064, 3768
MmeI TCCRAC 7 cut(s) 305, 1044, 1242, 1244, 1375, 2736, 3699
Mox20I TGGCCA 1 cut(s) 2314
Mph1103I ATGCAT 4 cut(s) 1025, 2878, 3020, 4029
MroXI GAANNNNTTC 2 cut(s) 2517, 3545
MscI TGGCCA 1 cut(s) 2314
MseI TTAA 8 cut(s) 864, 1175, 1346, 2172, 2642, 2670, 2709, 2927
MslI CAYNNNNRTG 4 cut(s) 239, 1340, 1422, 3978
Msp20I TGGCCA 1 cut(s) 2314
MspA1I CMGCKG 3 cut(s) 752, 1073, 1859
MspCI CTTAAG 2 cut(s) 2669, 2708
MspI CCGG 9 cut(s) 266, 285, 305, 1620, 1885, 3098, 3287, 3803, 3836
MspR9I CCNGG 6 cut(s) 306, 634, 1620, 1886, 2362, 3366
MunI CAATTG 2 cut(s) 2082, 2379
Mva1269I GAATGC 2 cut(s) 1832, 3427
MvaI CCWGG 3 cut(s) 634, 2362, 3366
MvnI CGCG 1 cut(s) 884
NciI CCSGG 3 cut(s) 306, 1620, 1886
NcoI CCATGG 2 cut(s) 411, 3190
NheI GCTAGC 1 cut(s) 1513
NlaIV GGNNCC 8 cut(s) 10, 297, 1068, 2354, 3233, 3370, 3371, 3890
NmeAIII GCCGAG 1 cut(s) 3474
NsbI TGCGCA 1 cut(s) 313
NsiI ATGCAT 4 cut(s) 1025, 2878, 3020, 4029
NspI RCATGY 4 cut(s) 1421, 2964, 3408, 3626
NspV TTCGAA 1 cut(s) 2245
PaeI GCATGC 1 cut(s) 3626
PaeR7I CTCGAG 1 cut(s) 3335
PagI TCATGA 2 cut(s) 1335, 2389
PciI ACATGT 2 cut(s) 1417, 3404
PctI GAATGC 2 cut(s) 1832, 3427
PdmI GAANNNNTTC 2 cut(s) 2517, 3545
PflFI GACNNNGTC 1 cut(s) 3758
PflMI CCANNNNNTGG 4 cut(s) 329, 517, 602, 1693
PfoI TCCNGGA 1 cut(s) 304
PinAI ACCGGT 1 cut(s) 3286
PleI GAGTC 4 cut(s) 171, 219, 3064, 3767
PpsI GAGTC 4 cut(s) 171, 219, 3064, 3767
Ppu21I YACGTR 1 cut(s) 3913
PpuMI RGGWCCY 1 cut(s) 3369
PscI ACATGT 2 cut(s) 1417, 3404
PshAI GACNNNNGTC 1 cut(s) 1409
PsiI TTATAA 1 cut(s) 1568
Psp5II RGGWCCY 1 cut(s) 3369
Psp6I CCWGG 3 cut(s) 632, 2360, 3364
PspEI GGTNACC 2 cut(s) 248, 1622
PspFI CCCAGC 1 cut(s) 423
PspGI CCWGG 3 cut(s) 632, 2360, 3364
PspN4I GGNNCC 8 cut(s) 10, 297, 1068, 2354, 3233, 3370, 3371, 3890
PspPI GGNCC 5 cut(s) 408, 1528, 2352, 3369, 3611
PspPPI RGGWCCY 1 cut(s) 3369
PspXI VCTCGAGB 1 cut(s) 3335
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 2 cut(s) 1066, 3512
PsyI GACNNNGTC 1 cut(s) 3758
PvuII CAGCTG 2 cut(s) 752, 1859
RsaI GTAC 9 cut(s) 10, 1080, 1272, 2177, 2209, 2984, 3146, 3483, 3911
RsaNI GTAC 9 cut(s) 9, 1079, 1271, 2176, 2208, 2983, 3145, 3482, 3910
RseI CAYNNNNRTG 4 cut(s) 239, 1340, 1422, 3978
SaqAI TTAA 8 cut(s) 864, 1175, 1346, 2172, 2642, 2670, 2709, 2927
Sau96I GGNCC 5 cut(s) 408, 1528, 2352, 3369, 3611
ScaI AGTACT 2 cut(s) 1272, 2984
SchI GAGTC 4 cut(s) 172, 220, 3064, 3768
ScrFI CCNGG 6 cut(s) 306, 634, 1620, 1886, 2362, 3366
SduI GDGCHC 7 cut(s) 1429, 1982, 2161, 3234, 3583, 3843, 3893
SfaNI GCATC 9 cut(s) 299, 1551, 2017, 2863, 2885, 2944, 3027, 3322, 3933
Sfr274I CTCGAG 1 cut(s) 3335
SfuI TTCGAA 1 cut(s) 2245
SinI GGWCC 4 cut(s) 408, 2352, 3369, 3611
SlaI CTCGAG 1 cut(s) 3335
SmiMI CAYNNNNRTG 4 cut(s) 239, 1340, 1422, 3978
SpeI ACTAGT 2 cut(s) 2912, 3592
SphI GCATGC 1 cut(s) 3626
SsiI CCGC 4 cut(s) 333, 1071, 3136, 3620
SspI AATATT 3 cut(s) 1723, 2447, 3871
StyD4I CCNGG 6 cut(s) 304, 632, 1618, 1884, 2360, 3364
StyI CCWWGG 3 cut(s) 411, 1369, 3190
TaaI ACNGT 6 cut(s) 114, 839, 904, 1366, 1643, 3611
TaiI ACGT 7 cut(s) 360, 737, 1683, 2763, 3033, 3699, 3915
TaqI TCGA 7 cut(s) 811, 933, 1239, 2245, 2804, 3043, 3336
TaqII GACCGA 2 cut(s) 396, 2249
TatI WGTACW 4 cut(s) 1270, 2982, 3144, 3481
TauI GCSGC 2 cut(s) 336, 3139
Tru1I TTAA 8 cut(s) 864, 1175, 1346, 2172, 2642, 2670, 2709, 2927
Tru9I TTAA 8 cut(s) 864, 1175, 1346, 2172, 2642, 2670, 2709, 2927
TscAI CASTG 9 cut(s) 117, 1166, 1369, 2019, 2372, 2770, 3326, 3746, 3862
TseI GCWGC 8 cut(s) 154, 314, 1169, 1856, 1859, 1946, 2052, 2634
TspGWI ACGGA 1 cut(s) 1238
TspRI CASTG 9 cut(s) 117, 1166, 1369, 2019, 2372, 2770, 3326, 3746, 3862
Tth111I GACNNNGTC 1 cut(s) 3758
Van91I CCANNNNNTGG 4 cut(s) 329, 517, 602, 1693
Vha464I CTTAAG 2 cut(s) 2669, 2708
VneI GTGCAC 1 cut(s) 1425
VpaK11BI GGWCC 4 cut(s) 408, 2352, 3369, 3611
XagI CCTNNNNNAGG 1 cut(s) 1535
XbaI TCTAGA 2 cut(s) 2189, 3583
XceI RCATGY 4 cut(s) 1421, 2964, 3408, 3626
XhoI CTCGAG 1 cut(s) 3335
XmnI GAANNNNTTC 2 cut(s) 2517, 3545
ZraI GACGTC 1 cut(s) 2761
ZrmI AGTACT 2 cut(s) 1272, 2984
Zsp2I ATGCAT 4 cut(s) 1025, 2878, 3020, 4029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.