Rw7G023150

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000085
Physical Location & Seq
Forward (+)
27743227 .. 27752499
9273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G023150.1

Sequence Viewer

Length: 4755 bp
ATGGATCTGAAGTTGGTTCGAGATGATCAGGATTGGGCGCGGACTTGGGTGTCCTTCGATCTTGGAGATGGTACAGTATCGTCGGCGGTGAACCACGGTAACGGCGGCTATGGGGATGGCGGTTTCCAGGGCGACGGAGCTCCTACTTGGGCTCTATGGGCTGGTGATCGTTCTGGGGATGGGGATGCTCAACGTGGTGGCTTTTTTGGCTATGCTGGTGATGGCCTGAAGGTGATCAGTAGGGGAGGCGGCGTCTTTGCCGATGACGGCGACGGCGACATGCAACTTGGGAGCGGCCATGTTGTGGAGCAGCTGGTTGTTAGGGTTGCAGAAAATTTGGGCTGTTGGGCTCAAAAAAATATGCCCACTACCAAACAAGTATACCGTGGAGAAAAATATGTCCACTACCAAACAAGAGATCTTTACCTTAGAATACCGACCGACAGAGAACTTGTTGATGCCATTGTGGACGATGTCTGCACTAGAGCACCACCTACAATTGAGTCCACCGAAGATATCATAGTATTTGAAGCAACAAGACGAGCCATGGATCAGCTTATGAAGGCATTAGAAGATAACAACGTCACTGCCGTTGGGGTGTACGGCATGGGGGGCGTTGGAAAGACGACCCTTGTGAAACATGTCGGTCAAGAAGCTCGGAAGCAAGGCCTTTTTGATCATGTCATTATGGGTAGAGTTAATCAAATCCCTGATTTGAAAAATATCCAAGGCATGTTGGCAGATGGGCTAGGCATCCAGTTGACGTACGAGACAGTGGATGGAAGAGCTTCTAGATTGTCTTCCGAGATAAGGAGAAGAAACAAAACCCTTATAATCTTGGACAACGTTTGGGCTAAAATAGAGCTGTCAGACATAGGAATTCCCACCTATGACATTCTTCAGAGTTGCAACTCCAAAGTCCTACTTACCACAAGGAGACAGGAAGTCTGTCATGTCATGAGGAGCCAAAAAGATATCGCCCTCAACTGCCTATCGAGAGAAGATTCATGGACATTGTTTGTGAAACATGCAAGCAAGTCTTTTGAATCAACCGATTCCGAGACTGTCGCAAGGAAGGTAGCTCGAGAATGTGGTGGTCTACCAATTGCATTGATAGCAGTTGCAAGGGAACACGCAGATGACCGGCTAGAGGAGTCGCTCGCTGCCAACCATGACCATGACAGACGTGCATACGAATGCATAAAATTAAGCTATGATTTCTTGAAAGATGAGGACTCCAAGTCATGCTTCTTGCTTTGTTGCCTATTCCCAGAAGGCTATGACATCCAAATAGAAGACTTGTTCAAGTATGGGATCGGGACAGGATTGTTTAAAAATGCTGCCACAATCCAACTAGCCAGACGAGAGGCTCGTTCAGTGGCCAAGAACCTTATACATTCTAGCTTGCTTTTGGATAGTGAATATGATGGATGTGTTAGGATGCATAATGTCATCCGGGATACAGCCATTCAAATTACACAATCTGAAGGTGAGCGCAGATTTTTCGTAGAAGCTGGCTGTCGTTTGGAGAATTGGCCACTTCAATTGCATGAAGACCACTCTGCAATTTCACTCATGAAGAACGACATTTGCAAGCTACCTGAAGAGTTGGTATGTCCGAAACTCCAAATTTTATTGCTACAAGAGAATCATGATTTAGATCAGATCCCTGATACTTTTTTTGAAACTACTAGTTCACTAAGAGTCGTAGATCTTAGTTACACTAGTATATCATCCCTATCTAAGTCATTTACTCTCCTAAGCAATCTCAAAGGTTTGTATTTGGATTATTGCCGGAACATAGCTGACATTTCCATACTGAAAAAATGTAAGAAGCTTGAGATTCTCAGTATGAAAGAATACCCTCTAAAGGAATTGTCAACAGAAATAGGAAATTTGACCAATTTAAGGGTGTTGGATGTTAGTGGTCCAGTCGGTGGAGGTATCATCAGAATTCCATCTGGGGTGATATCAAAATTGCTTAAGTTAGAAGAAATATGCCTGCACTGTGGATTTTGGGACTGGGGGAGTAGAGTTGAGGGAGAAGGAGAAACAACTAATGCTAGCTTTGATGAGGTAACTAGCTTATCATATTTGAACAGCTTGAAGGTTTGCATATCTGATGTGAATTGCATCCCTAGAAATGTTAAAAGGGTTTGGGACAAGATGAGTTGGACTTGCTTTGATATATGTATTGGTAGGGACTTGAACGCCAGTACTATTATCAGAACTGGTAGAGGTAGAGACAGGGACCCCAAAAATATTCTCAGAGACCTTGGTAGATGTGTTGATAGAGAGAAGAACACCAGAAATATTATCAGAGACCTGCGCACTCAATTCAGGAGAGGTGATCATAAATATTCAAGATCTTTGATTCTTGATACAACCATTAGTATGTTACCATCTTGGTTTGTCAAGGTGGTGACAGAGAAGACAGAGAGGCTAGTGTATGTGAATTTCACAGGGTTAAATAGCATCTTCATGGAATATCACCATGGGAGGTTACATAGTCTGAAGCATCTCTCTGTTATTGGTCCCGATGAGAGCTTGAAAGCATTGATCCTTGTGGACATAACAGGAAAGGATCAAAATAAAGCTGTGTTCGAGACATTGGAAGAGTTGCATGTGGGCGGTCTGGATTCCTTGAAGGAGTTATGTTCTGGTGAATTACCATCTGGGTCTCTCTTCAATCTGAAAGTATTGGAAGTACGTGATTGTCCTGACTTGGAAAATGTCCTGTTACCATCAAAATTGTTACAGAGACTGGCAAATCTGGAGAACCTACTTTGTAAGTGTATGTCTGGAATGGAATATGTGTTTGGATGTGAAGGATTTGAGCTAGAAGAATCAAAACTGAAAAAGATGGAGTTGTTTCAGCTAGAAGCAGTCAAGAGCATATGTAATGGTCCTGTTCCACCCGCAATGTTCCAAGCACTTCAGAGTTTGTCCATTTACGATTGCAACTTTCTAGGAGGTCTCTTCACATTTGATGTAGCTCAATGTCTTTTTCAAGTGGAAAACATTTCAGTAGTTAGTTGCCGTAACTTGGAAAGAGTAATCGAAGCAAGCAAGGAATCAGAGCTCAGAAAGAAGACCGTTCTTCCAAAATTGAAGTCCCTAGTCTTGGTGAATCTTCCAATGTTGTACAGTGGTGGTGCTGCTATTGATATGGAGTGTCCTTCATTGCAACACTTGTATGTGAGAGACTGCCCTCATTTTTCAGCCTCTGCTGATGACTTCGGGAGCAATAACAAAGTCGTAGTCAACGATGTACTGCATTACTGTACTCAAACCACAAGACTCCAAAAACCAGGGGATTATTCCGACTCATATCTCGAGGCCGAGGAAAAGATCCGTACAATGATGGAGAGTCAGTTGGAGCCGACTATGCCTAACTTGGCAAAGGGGCTTGCTGAAATCCTCCTGAGTTCACCTCAAGGCATGCAGAATATGCCCAAATTTGAGGACGGTAAATGCTTAGAGAACAAGATGAAGGTTGCTGGATTGACGTGGAAGACGCCCGGAGCGATGGCGTATGTAATTGGAGCGTTCGTGAAGATAAACCCTGAAGCGTTGAATCAAATCCCTGAATTAATGGATGAGCTGCAATCGATGGATGGACTGCTGGACGACAATCCGCCGGAGGAGGAAAAGCCAGACGACCTATTCGCTGATGATATGCCCATGCCCGAGTTTAGAAGGGTAATGGAGCCTGTAGCTGAGATTAAATATGGAAGTGCACTTGACGGTGAATCTGAGAGTGCATTTGACATTGACAGTCAATCTGACAGGGCACTTCTGAGTGAATTGATAGGGCACTTCTGGAGTCGAAAACCAGGGGATTGTTCCGACTCATATCTCGAGGCCGAGGAAAAGATCCGTACATTGATGGAGAATCAATTGGAGCCGACTATGCATGACTGGAGAAAGGGGTTTGCTGAATTTTTGTATAACACACCTCAAGCCAAGCAGAATATGCCCAAATTTGAGGACGGTAAATGGTTAGAGAACAAGATGAAGCTATTTGAATTGATCAGGAAGACGCCCGGAGCGATGGAGTATATAATTGGAGTGTTCGTGAAAATGAATCCTGAAGCGTTGAATCATGTCCCTGAATACATGGACATGATGCAATCGATGGAGGGACTGCTGGACGACAATCCGCCTGAGGATAAGCCAGACAACCTATCCGCTGATAAGACGTTATTTCCCGAGTTTAAAAGGGTAATGGAGCGTGTAGCTGAGACTAAATATGGAAGTGCAAGTGCATTTGACATTGACAGTGAATCTGACAGGGCACTTCTGAGTGAATTAATCAGTGCATCTCAGAGTTTATCCATCCGAGACCGTGAATCTGGGAGTGCATCGTCAAGTGCATTTGACAGTGAATTTATCAGTGTATTGCCAAAGGCATTTCAGATTGCCAAGTGCATTGAAGAGTGCTTCCCTCGACACAGTGAATCTGAGAGTGCATTTGACAGTGAATCTATCAGTGCATTTCAGAGTTCATCCATCCGAGACAGTCAATCTAAGAGTGCATCGTCAAGTGCATTCGACATTGATTCATTGTCAAGTGCAATTCAGAGTTTATCCATGCAAGACCGTGAATCTGAGAGTGCATCGTCAAGTGCATTTGATAGTGAATTTATCAGTGCAATGTCAGGTGGATTTCAGCTTATCAGTGGATTGGAAAGTGACAGTAAATTTTGCAGTGGAATGAAAAGGGCATTTCAGAGTTCATCCAGCAAGAAATGA

Protein Analysis

1584

Amino Acids

177.09

Weight (kDa)

5.06

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 182 - 345 6.1e-27 NB-ARC domain
LRR_8 PF13855 540 - 599 2.7e-09 Leucine rich repeat
LRR_14 PF23598 553 - 643 1.9e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 872 - 1014 2.1e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 967 - 1073 1.3e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 833
AasI GACNNNNNNGTC 1 cut(s) 49
Acc16I TGCGCA 1 cut(s) 2330
Acc36I ACCTGC 1 cut(s) 2334
AccB7I CCANNNNNTGG 2 cut(s) 304, 1937
AccBSI CCGCTC 1 cut(s) 294
AccI GTMKAC 2 cut(s) 381, 1099
AccII CGCG 1 cut(s) 40
AclI AACGTT 1 cut(s) 846
AclWI GGATC 8 cut(s) 12, 558, 1322, 1660, 2554, 2592, 3346, 3871
AcoI YGGCCR 3 cut(s) 295, 1380, 1535
AcuI CTGAAG 9 cut(s) 29, 248, 884, 1506, 1623, 2534, 2921, 3588, 4113
AcyI GRCGYC 3 cut(s) 252, 3518, 4043
AfiI CCNNNNNNNGG 8 cut(s) 304, 810, 1150, 1870, 1908, 1937, 3046, 3124
AflII CTTAAG 1 cut(s) 1982
AflIII ACRYGT 1 cut(s) 640
AhdI GACNNNNNGTC 2 cut(s) 944, 1240
AhlI ACTAGT 2 cut(s) 1691, 1724
AjiI CACGTC 2 cut(s) 1187, 3510
AjnI CCWGG 3 cut(s) 126, 3310, 3835
AjuI GAANNNNNNNTTGG 4 cut(s) 1232, 1264, 2802, 2834
Alw21I GWGCWC 4 cut(s) 142, 490, 3084, 3742
Alw44I GTGCAC 1 cut(s) 3738
AlwI GGATC 8 cut(s) 12, 558, 1322, 1660, 2554, 2592, 3346, 3871
AlwNI CAGNNNCTG 2 cut(s) 2764, 3227
Ama87I CYCGRG 5 cut(s) 1083, 3335, 3689, 3860, 4211
AoxI GGCC 7 cut(s) 223, 295, 667, 1380, 1535, 3339, 3864
ApaLI GTGCAC 1 cut(s) 3738
ApeKI GCWGC 5 cut(s) 310, 1163, 1340, 3158, 3604
ArsI GACNNNNNNTTYG 6 cut(s) 3098, 3130, 3246, 3278, 3650, 3682
AseI ATTAAT 2 cut(s) 3593, 4313
Asp700I GAANNNNTTC 1 cut(s) 787
AspLEI GCGC 3 cut(s) 40, 1497, 2331
AspS9I GGNCC 4 cut(s) 1928, 2251, 2534, 2906
AsuC2I CCSGG 3 cut(s) 1457, 3522, 4047
AsuNHI GCTAGC 1 cut(s) 2063
AvaI CYCGRG 5 cut(s) 1083, 3335, 3689, 3860, 4211
AvaII GGWCC 4 cut(s) 1928, 2251, 2534, 2906
AxyI CCTNAGG 1 cut(s) 4167
BaeGI GKGCMC 4 cut(s) 3742, 3796, 3819, 4300
BalI TGGCCA 2 cut(s) 1382, 1537
BanII GRGCYC 4 cut(s) 142, 154, 352, 3084
BbsI GAAGAC 7 cut(s) 792, 1302, 1560, 2438, 3098, 3521, 4046
Bbv12I GWGCWC 4 cut(s) 142, 490, 3084, 3742
BbvI GCAGC 5 cut(s) 322, 1150, 1327, 3145, 3591
BceAI ACGGC 6 cut(s) 118, 283, 289, 575, 619, 3024
BcgI CGANNNNNNTGC 2 cut(s) 1486, 1520
BciT130I CCWGG 3 cut(s) 128, 3312, 3837
BciVI GTATCC 1 cut(s) 1453
BclI TGATCA 5 cut(s) 25, 234, 676, 2350, 4032
BcnI CCSGG 3 cut(s) 1457, 3522, 4047
BcuI ACTAGT 2 cut(s) 1691, 1724
BfmI CTRYAG 1 cut(s) 3714
BfrI CTTAAG 1 cut(s) 1982
BfuAI ACCTGC 1 cut(s) 2334
BfuI GTATCC 1 cut(s) 1453
BglII AGATCT 3 cut(s) 418, 1711, 2366
BisI GCNGC 8 cut(s) 106, 250, 295, 311, 1164, 1341, 3159, 3605
BlsI GCNGC 8 cut(s) 107, 251, 296, 312, 1165, 1342, 3160, 3606
BmcAI AGTACT 1 cut(s) 2218
Bme1390I CCNGG 6 cut(s) 128, 1457, 3312, 3522, 3837, 4047
Bme18I GGWCC 4 cut(s) 1928, 2251, 2534, 2906
BmeRI GACNNNNNGTC 2 cut(s) 944, 1240
BmeT110I CYCGRG 5 cut(s) 1083, 3335, 3689, 3860, 4211
BmgBI CACGTC 2 cut(s) 1187, 3510
BmgT120I GGNCC 4 cut(s) 1928, 2251, 2534, 2906
BmiI GGNNCC 7 cut(s) 965, 2252, 2253, 2536, 3381, 3711, 3906
BmrFI CCNGG 6 cut(s) 128, 1457, 3312, 3522, 3837, 4047
BmrI ACTGGG 1 cut(s) 2032
BmtI GCTAGC 1 cut(s) 2067
BmuI ACTGGG 1 cut(s) 2032
BpiI GAAGAC 7 cut(s) 792, 1302, 1560, 2438, 3098, 3521, 4046
BplI GAGNNNNNCTC 2 cut(s) 3418, 3450
BpmI CTGGAG 3 cut(s) 2795, 3844, 3943
Bpu10I CCTNAGC 1 cut(s) 1760
BpuEI CTTGAG 3 cut(s) 1859, 3420, 3945
BpuMI CCSGG 3 cut(s) 1457, 3522, 4047
Bsa29I ATCGAT 2 cut(s) 3611, 4136
BsaAI YACGTR 1 cut(s) 2711
BsaBI GATNNNNATC 1 cut(s) 1659
BsaHI GRCGYC 3 cut(s) 252, 3518, 4043
BsaI GGTCTC 5 cut(s) 2265, 2316, 2685, 2981, 4338
BsaXI ACNNNNNCTCC 8 cut(s) 237, 267, 3359, 3389, 3537, 3567, 4062, 4092
Bsc4I CCNNNNNNNGG 8 cut(s) 304, 810, 1150, 1870, 1908, 1937, 3046, 3124
Bse118I RCCGGY 1 cut(s) 1143
Bse1I ACTGG 7 cut(s) 757, 1931, 2027, 2214, 2236, 2769, 3926
Bse21I CCTNAGG 1 cut(s) 4167
Bse3DI GCAATG 3 cut(s) 2928, 3182, 4662
Bse8I GATNNNNATC 1 cut(s) 1659
BseBI CCWGG 3 cut(s) 128, 3312, 3837
BseCI ATCGAT 2 cut(s) 3611, 4136
BseJI GATNNNNATC 1 cut(s) 1659
BseLI CCNNNNNNNGG 8 cut(s) 304, 810, 1150, 1870, 1908, 1937, 3046, 3124
BseMI GCAATG 3 cut(s) 2928, 3182, 4662
BseNI ACTGG 7 cut(s) 757, 1931, 2027, 2214, 2236, 2769, 3926
BseRI GAGGAG 3 cut(s) 976, 1166, 3659
BseSI GKGCMC 4 cut(s) 3742, 3796, 3819, 4300
BseXI GCAGC 5 cut(s) 322, 1150, 1327, 3145, 3591
BsgI GTGCAG 2 cut(s) 463, 1988
Bsh1236I CGCG 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 7 cut(s) 225, 297, 669, 1382, 1537, 3341, 3866
BshVI ATCGAT 2 cut(s) 3611, 4136
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 4 cut(s) 142, 490, 3084, 3742
BsiHKCI CYCGRG 5 cut(s) 1083, 3335, 3689, 3860, 4211
BsiSI CCGG 6 cut(s) 1144, 1456, 1795, 3522, 3641, 4047
BsiWI CGTACG 1 cut(s) 765
BslFI GGGAC 9 cut(s) 1333, 2033, 2174, 2216, 2264, 2520, 3100, 4094, 4158
BslI CCNNNNNNNGG 8 cut(s) 304, 810, 1150, 1870, 1908, 1937, 3046, 3124
BsmFI GGGAC 9 cut(s) 1333, 2033, 2174, 2216, 2264, 2520, 3100, 4094, 4158
BsmI GAATGC 2 cut(s) 1202, 4550
BsnI GGCC 7 cut(s) 225, 297, 669, 1382, 1537, 3341, 3866
Bso31I GGTCTC 5 cut(s) 2265, 2316, 2685, 2981, 4338
BsoBI CYCGRG 5 cut(s) 1083, 3335, 3689, 3860, 4211
Bsp1286I GDGCHC 9 cut(s) 142, 154, 352, 490, 3084, 3742, 3796, 3819, 4300
Bsp1407I TGTACA 1 cut(s) 3144
Bsp19I CCATGG 2 cut(s) 546, 2494
BspANI GGCC 7 cut(s) 225, 297, 669, 1382, 1537, 3341, 3866
BspDI ATCGAT 2 cut(s) 3611, 4136
BspFNI CGCG 1 cut(s) 40
BspHI TCATGA 3 cut(s) 957, 1575, 1651
BspLI GGNNCC 7 cut(s) 965, 2252, 2253, 2536, 3381, 3711, 3906
BspMI ACCTGC 1 cut(s) 2334
BspOI GCTAGC 1 cut(s) 2067
BspPI GGATC 8 cut(s) 12, 558, 1322, 1660, 2554, 2592, 3346, 3871
BspQI GCTCTTC 1 cut(s) 778
BspTI CTTAAG 1 cut(s) 1982
BspTNI GGTCTC 5 cut(s) 2265, 2316, 2685, 2981, 4338
BsrBI CCGCTC 1 cut(s) 294
BsrDI GCAATG 3 cut(s) 2928, 3182, 4662
BsrFI RCCGGY 1 cut(s) 1143
BsrGI TGTACA 1 cut(s) 3144
BsrI ACTGG 7 cut(s) 757, 1931, 2027, 2214, 2236, 2769, 3926
BssAI RCCGGY 1 cut(s) 1143
BssNAI GTATAC 1 cut(s) 382
BssNI GRCGYC 3 cut(s) 252, 3518, 4043
BssT1I CCWWGG 4 cut(s) 546, 727, 2275, 2494
Bst1107I GTATAC 1 cut(s) 382
Bst2UI CCWGG 3 cut(s) 128, 3312, 3837
Bst6I CTCTTC 6 cut(s) 778, 1599, 2610, 2690, 2984, 4431
BstACI GRCGYC 3 cut(s) 252, 3518, 4043
BstAFI CTTAAG 1 cut(s) 1982
BstAPI GCANNNNNTGC 2 cut(s) 3451, 3976
BstAUI TGTACA 1 cut(s) 3144
BstBAI YACGTR 1 cut(s) 2711
BstDSI CCRYGG 4 cut(s) 94, 385, 546, 2494
BstFNI CGCG 1 cut(s) 40
BstHHI GCGC 3 cut(s) 40, 1497, 2331
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 8 cut(s) 158, 207, 612, 1115, 3388, 3451, 3913, 3976
BstNI CCWGG 3 cut(s) 128, 3312, 3837
BstNSI RCATGY 6 cut(s) 283, 644, 736, 1031, 2627, 3445
BstSCI CCNGG 6 cut(s) 126, 1455, 3310, 3520, 3835, 4045
BstSFI CTRYAG 1 cut(s) 3714
BstSLI GKGCMC 4 cut(s) 3742, 3796, 3819, 4300
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 5 cut(s) 322, 1150, 1327, 3145, 3591
BstV2I GAAGAC 7 cut(s) 792, 1302, 1560, 2438, 3098, 3521, 4046
BstX2I RGATCY 7 cut(s) 4, 418, 1665, 1711, 2366, 3351, 3876
BstYI RGATCY 7 cut(s) 4, 418, 1665, 1711, 2366, 3351, 3876
BstZ17I GTATAC 1 cut(s) 382
Bsu15I ATCGAT 2 cut(s) 3611, 4136
Bsu36I CCTNAGG 1 cut(s) 4167
BsuI GTATCC 1 cut(s) 1453
BsuRI GGCC 7 cut(s) 225, 297, 669, 1382, 1537, 3341, 3866
BsuTUI ATCGAT 2 cut(s) 3611, 4136
BtgI CCRYGG 4 cut(s) 94, 385, 546, 2494
BtgZI GCGATG 2 cut(s) 3542, 4067
BtrI CACGTC 2 cut(s) 1187, 3510
BtsI GCAGTG 2 cut(s) 585, 4717
BveI ACCTGC 1 cut(s) 2334
CaiI CAGNNNCTG 2 cut(s) 2764, 3227
CciI TCATGA 3 cut(s) 957, 1575, 1651
CfoI GCGC 3 cut(s) 40, 1497, 2331
Cfr10I RCCGGY 1 cut(s) 1143
Cfr13I GGNCC 4 cut(s) 1928, 2251, 2534, 2906
ClaI ATCGAT 2 cut(s) 3611, 4136
CseI GACGC 3 cut(s) 241, 3526, 4051
CspCI CAANNNNNGTGG 2 cut(s) 1527, 1562
DraI TTTAAA 2 cut(s) 1333, 4219
DrdI GACNNNNNNGTC 1 cut(s) 49
DriI GACNNNNNGTC 2 cut(s) 944, 1240
DseDI GACNNNNNNGTC 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 295, 1380, 1535
Eam1104I CTCTTC 6 cut(s) 778, 1599, 2610, 2690, 2984, 4431
Eam1105I GACNNNNNGTC 2 cut(s) 944, 1240
EarI CTCTTC 6 cut(s) 778, 1599, 2610, 2690, 2984, 4431
EciI GGCGGA 2 cut(s) 3627, 4152
Ecl136II GAGCTC 2 cut(s) 140, 3082
Eco130I CCWWGG 4 cut(s) 546, 727, 2275, 2494
Eco147I AGGCCT 1 cut(s) 669
Eco24I GRGCYC 4 cut(s) 142, 154, 352, 3084
Eco31I GGTCTC 5 cut(s) 2265, 2316, 2685, 2981, 4338
Eco32I GATATC 3 cut(s) 517, 976, 1971
Eco47I GGWCC 4 cut(s) 1928, 2251, 2534, 2906
Eco53kI GAGCTC 2 cut(s) 140, 3082
Eco57I CTGAAG 9 cut(s) 29, 248, 884, 1506, 1623, 2534, 2921, 3588, 4113
Eco81I CCTNAGG 1 cut(s) 4167
Eco88I CYCGRG 5 cut(s) 1083, 3335, 3689, 3860, 4211
EcoICRI GAGCTC 2 cut(s) 140, 3082
EcoO109I RGGNCCY 1 cut(s) 2251
EcoRI GAATTC 2 cut(s) 879, 1953
EcoRII CCWGG 3 cut(s) 126, 3310, 3835
EcoRV GATATC 3 cut(s) 517, 976, 1971
EcoT14I CCWWGG 4 cut(s) 546, 727, 2275, 2494
EcoT22I ATGCAT 3 cut(s) 1202, 1446, 3918
EcoT38I GRGCYC 4 cut(s) 142, 154, 352, 3084
ErhI CCWWGG 4 cut(s) 546, 727, 2275, 2494
FalI AAGNNNNNCTT 6 cut(s) 909, 941, 1024, 1056, 1232, 1264
FaqI GGGAC 9 cut(s) 1333, 2033, 2174, 2216, 2264, 2520, 3100, 4094, 4158
FauI CCCGC 1 cut(s) 2926
FauNDI CATATG 1 cut(s) 2897
FbaI TGATCA 5 cut(s) 25, 234, 676, 2350, 4032
FblI GTMKAC 2 cut(s) 381, 1099
Fnu4HI GCNGC 8 cut(s) 106, 250, 295, 311, 1164, 1341, 3159, 3605
FriOI GRGCYC 4 cut(s) 142, 154, 352, 3084
Fsp4HI GCNGC 8 cut(s) 106, 250, 295, 311, 1164, 1341, 3159, 3605
FspI TGCGCA 1 cut(s) 2330
GlaI GCGC 3 cut(s) 39, 1496, 2330
GluI GCNGC 8 cut(s) 106, 250, 295, 311, 1164, 1341, 3159, 3605
GsuI CTGGAG 3 cut(s) 2795, 3844, 3943
HaeIII GGCC 7 cut(s) 225, 297, 669, 1382, 1537, 3341, 3866
HapII CCGG 6 cut(s) 1144, 1456, 1795, 3522, 3641, 4047
HgaI GACGC 3 cut(s) 241, 3526, 4051
HhaI GCGC 3 cut(s) 40, 1497, 2331
Hin1I GRCGYC 3 cut(s) 252, 3518, 4043
Hin6I GCGC 3 cut(s) 38, 1495, 2329
HinP1I GCGC 3 cut(s) 38, 1495, 2329
HincII GTYRAC 3 cut(s) 762, 1881, 3265
HindII GTYRAC 3 cut(s) 762, 1881, 3265
HindIII AAGCTT 1 cut(s) 1835
HpaII CCGG 6 cut(s) 1144, 1456, 1795, 3522, 3641, 4047
Hpy99I CGWCG 3 cut(s) 85, 137, 275
HpyCH4IV ACGT 8 cut(s) 193, 582, 764, 846, 1186, 2710, 3509, 4202
HpyF10VI GCNNNNNNNGC 8 cut(s) 158, 207, 612, 1115, 3388, 3451, 3913, 3976
HpySE526I ACGT 8 cut(s) 193, 582, 764, 846, 1186, 2710, 3509, 4202
Hsp92I GRCGYC 3 cut(s) 252, 3518, 4043
HspAI GCGC 3 cut(s) 38, 1495, 2329
KflI GGGWCCC 1 cut(s) 2251
Ksp22I TGATCA 5 cut(s) 25, 234, 676, 2350, 4032
LguI GCTCTTC 1 cut(s) 778
Lsp1109I GCAGC 5 cut(s) 322, 1150, 1327, 3145, 3591
MaeII ACGT 8 cut(s) 193, 582, 764, 846, 1186, 2710, 3509, 4202
MbiI CCGCTC 1 cut(s) 294
MfeI CAATTG 4 cut(s) 498, 1104, 1544, 3899
MflI RGATCY 7 cut(s) 4, 418, 1665, 1711, 2366, 3351, 3876
MhlI GDGCHC 9 cut(s) 142, 154, 352, 490, 3084, 3742, 3796, 3819, 4300
MlsI TGGCCA 2 cut(s) 1382, 1537
MluNI TGGCCA 2 cut(s) 1382, 1537
MlyI GAGTC 9 cut(s) 512, 1163, 1229, 1713, 3294, 3320, 3379, 3835, 3845
MmeI TCCRAC 7 cut(s) 598, 1375, 1896, 2153, 3348, 3357, 3873
Mox20I TGGCCA 2 cut(s) 1382, 1537
Mph1103I ATGCAT 3 cut(s) 1202, 1446, 3918
MroXI GAANNNNTTC 1 cut(s) 787
MscI TGGCCA 2 cut(s) 1382, 1537
MslI CAYNNNNRTG 7 cut(s) 1137, 1176, 1195, 2393, 2480, 3195, 4124
Msp20I TGGCCA 2 cut(s) 1382, 1537
MspA1I CMGCKG 2 cut(s) 313, 4193
MspCI CTTAAG 1 cut(s) 1982
MspI CCGG 6 cut(s) 1144, 1456, 1795, 3522, 3641, 4047
MspR9I CCNGG 6 cut(s) 128, 1457, 3312, 3522, 3837, 4047
MunI CAATTG 4 cut(s) 498, 1104, 1544, 3899
Mva1269I GAATGC 2 cut(s) 1202, 4550
MvaI CCWGG 3 cut(s) 128, 3312, 3837
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 8 cut(s) 158, 207, 612, 1115, 3388, 3451, 3913, 3976
NciI CCSGG 3 cut(s) 1457, 3522, 4047
NcoI CCATGG 2 cut(s) 546, 2494
NdeI CATATG 1 cut(s) 2897
NheI GCTAGC 1 cut(s) 2063
NlaIV GGNNCC 7 cut(s) 965, 2252, 2253, 2536, 3381, 3711, 3906
NmeAIII GCCGAG 2 cut(s) 3367, 3892
NmuCI GTSAC 3 cut(s) 583, 2422, 4694
NsbI TGCGCA 1 cut(s) 2330
NsiI ATGCAT 3 cut(s) 1202, 1446, 3918
NspI RCATGY 6 cut(s) 283, 644, 736, 1031, 2627, 3445
PaeI GCATGC 1 cut(s) 3445
PaeR7I CTCGAG 3 cut(s) 1083, 3335, 3860
PagI TCATGA 3 cut(s) 957, 1575, 1651
PceI AGGCCT 1 cut(s) 669
PciI ACATGT 1 cut(s) 640
PciSI GCTCTTC 1 cut(s) 778
PcsI WCGNNNNNNNCGW 6 cut(s) 273, 588, 1369, 3264, 3524, 4049
PctI GAATGC 2 cut(s) 1202, 4550
PdmI GAANNNNTTC 1 cut(s) 787
Pfl23II CGTACG 1 cut(s) 765
PflFI GACNNNGTC 1 cut(s) 473
PflMI CCANNNNNTGG 2 cut(s) 304, 1937
PfoI TCCNGGA 1 cut(s) 1455
PkrI GCNGC 8 cut(s) 107, 251, 296, 312, 1165, 1342, 3160, 3606
PleI GAGTC 9 cut(s) 511, 1162, 1229, 1712, 3294, 3320, 3378, 3834, 3845
PpsI GAGTC 9 cut(s) 511, 1162, 1229, 1712, 3294, 3320, 3378, 3834, 3845
Ppu21I YACGTR 1 cut(s) 2711
PpuMI RGGWCCY 1 cut(s) 2251
PscI ACATGT 1 cut(s) 640
PshBI ATTAAT 2 cut(s) 3593, 4313
PsiI TTATAA 1 cut(s) 833
Psp124BI GAGCTC 2 cut(s) 142, 3084
Psp1406I AACGTT 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 2251
Psp6I CCWGG 3 cut(s) 126, 3310, 3835
PspGI CCWGG 3 cut(s) 126, 3310, 3835
PspLI CGTACG 1 cut(s) 765
PspN4I GGNNCC 7 cut(s) 965, 2252, 2253, 2536, 3381, 3711, 3906
PspPI GGNCC 4 cut(s) 1928, 2251, 2534, 2906
PspPPI RGGWCCY 1 cut(s) 2251
PstNI CAGNNNCTG 2 cut(s) 2764, 3227
PsuI RGATCY 7 cut(s) 4, 418, 1665, 1711, 2366, 3351, 3876
PsyI GACNNNGTC 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 313
RseI CAYNNNNRTG 7 cut(s) 1137, 1176, 1195, 2393, 2480, 3195, 4124
SacI GAGCTC 2 cut(s) 142, 3084
SapI GCTCTTC 1 cut(s) 778
SatI GCNGC 8 cut(s) 106, 250, 295, 311, 1164, 1341, 3159, 3605
Sau96I GGNCC 4 cut(s) 1928, 2251, 2534, 2906
ScaI AGTACT 1 cut(s) 2218
SchI GAGTC 9 cut(s) 512, 1163, 1229, 1713, 3294, 3320, 3379, 3835, 3845
ScrFI CCNGG 6 cut(s) 128, 1457, 3312, 3522, 3837, 4047
SduI GDGCHC 9 cut(s) 142, 154, 352, 490, 3084, 3742, 3796, 3819, 4300
SfcI CTRYAG 1 cut(s) 3714
Sfr274I CTCGAG 3 cut(s) 1083, 3335, 3860
SinI GGWCC 4 cut(s) 1928, 2251, 2534, 2906
SlaI CTCGAG 3 cut(s) 1083, 3335, 3860
SmiMI CAYNNNNRTG 7 cut(s) 1137, 1176, 1195, 2393, 2480, 3195, 4124
SmlI CTYRAG 7 cut(s) 1083, 1838, 1982, 3335, 3435, 3860, 3960
SmoI CTYRAG 7 cut(s) 1083, 1838, 1982, 3335, 3435, 3860, 3960
SpeI ACTAGT 2 cut(s) 1691, 1724
SphI GCATGC 1 cut(s) 3445
SseBI AGGCCT 1 cut(s) 669
SspI AATATT 3 cut(s) 2263, 2314, 2360
SstI GAGCTC 2 cut(s) 142, 3084
StuI AGGCCT 1 cut(s) 669
StyD4I CCNGG 6 cut(s) 126, 1455, 3310, 3520, 3835, 4045
StyI CCWWGG 4 cut(s) 546, 727, 2275, 2494
TaiI ACGT 8 cut(s) 196, 585, 767, 849, 1189, 2713, 3512, 4205
TaqII GACCGA 2 cut(s) 455, 635
TatI WGTACW 4 cut(s) 2216, 3144, 3271, 3284
TauI GCSGC 3 cut(s) 108, 252, 297
TseFI GTSAC 3 cut(s) 583, 2422, 4694
TseI GCWGC 5 cut(s) 310, 1163, 1340, 3158, 3604
Tsp45I GTSAC 3 cut(s) 583, 2422, 4694
TspGWI ACGGA 3 cut(s) 150, 3344, 3869
Tth111I GACNNNGTC 1 cut(s) 473
Van91I CCANNNNNTGG 2 cut(s) 304, 1937
Vha464I CTTAAG 1 cut(s) 1982
VneI GTGCAC 1 cut(s) 3738
VpaK11BI GGWCC 4 cut(s) 1928, 2251, 2534, 2906
VspI ATTAAT 2 cut(s) 3593, 4313
XbaI TCTAGA 1 cut(s) 791
XceI RCATGY 6 cut(s) 283, 644, 736, 1031, 2627, 3445
XhoI CTCGAG 3 cut(s) 1083, 3335, 3860
XmiI GTMKAC 2 cut(s) 381, 1099
XmnI GAANNNNTTC 1 cut(s) 787
ZrmI AGTACT 1 cut(s) 2218
Zsp2I ATGCAT 3 cut(s) 1202, 1446, 3918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.