Rmu_sc0000595.1_g000029

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000595.1
OG0000085
Physical Location & Seq
Reverse (-)
131072 .. 136158
5087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000595.1_g000029.1.cds

Sequence Viewer

Length: 4656 bp
atggcctggttctctcgaaaatcctggttctctcgaaaatctgaggcatctcttccttcgtcagctgaaccatcatcaacagctcatcagtggaggtatgatgtgtttgtgagtaagggtcttgacactggaaggggctttacatcccatatacgcgatcggctggaagagacaggaatcacaacattaatgttaagggatggatttatggccactctccgagaggcaattgaagaatcaaggttcgtgattgttgttctgtccaaaaattatgcttcttctgcttggtgtttggatgaacttacagagatttgtcaattcacggaggacggccacagaattcttccagttttttatgaggtcgaccctagtgaagtaagaaatcaaacggggagttttttttatgctttcaccaagcataaaaggtccgggcgatacggatcggagaaggtccagcagtggagagatgccttgagaaaagtgggcagtttgtctgggtggaatacactgaattacaggactgataatggtcttgtccaagccattgtgaaatctgtgcgcagtaaactacaacctgttgtggaaatcaacacgggagattttgaagcatttgaagcaacaagacaagccactattgaaattgaaccatcatcaacagcccgtgggtggaagtatgatgtgtttgtgagtaagggtcttgacactggaaggggctttacatcccatttacacaattggctgcaaaagagaggaatcaaaacattcatggatagttcacagcattcatggctgcaaaagagggatgctatttctccctctctcctaaaggcaattgaaaaatcaaggttggcaattgttgttctgtccaaaaattatgcttcttctgcttggcgtttggatgaacttgcagagatttgtcagttcatggaggacggccacagaattcttccagttttttatgaggtcgaccctagtgaagtaagaaatcaaacggggagttttttttatgctttcaccaagcatgaaaggtctgggcgatacggatcggagaaggtccagcagtggagagatgctttaacaaaagtgggcagtttgtctttgtggaatacagtgaattacaggactgacaatgatcttgtccaagccatcgtgaaatctgtgtgcagtaaactagaacctgtttcggaaatcaacacgggagattttgaagcatttgaagcgacaagacaagccactattgaaattaagcctacaatgtcggagtcaaatgaaaactttgaagtagttaaagtaataagaggagctacaactgaaattgagcctacaatgccaaagttgactgaagattttgaagcaacaacacaagcgactaatggaaactctgaaccagctcctcagtggaagtatgatgtgtttttgagcttcaggggtgatgacactcgcaagggttttctatcacatttataccatgaattgcaaaacacaaaagtaattagaacatttaaggatgatgaacaactcaagaaagggaaggatatttctcaaggtctgctgacggcaattgaagaatcaaggtttgcgatcattgttctatcggaaaactatgctttttctgcatggtgtttagatgaacttacaaagatttttcaatgcatggaaggcaaggatacaattctgccaattttttatcatgtggatccctctgatgtacgatatcaaaacaataaactcgaagtggcctttacaaagcattcaagcaagggaaggtatggcatagagaagatcaagcagtggagagctgatttaaaaaaagtgggaaatctctctgggtgggattcaaagaaccacaaatctgagagggaactcgttgaagacattgtgaaatttgtgctcaaagaagtacttcaagcaccaaggggagattttgaagaatttgaagcaacaagagaagccatggatgaggttaagaagatgctgaaagatgataagatcactgccattggagtctatggaagagaaggcgttggcaagacagccatggtgaaacatgtcagtgcacaagccaaaagaattgggctttttgaccatgttgttatggctgttatatcccgaagccctgatttgcaaaaaattcaagatgcattggtagatcagctgggattagaattggtggaggatgatgaacaaatggaacgagccagtagattgaaggagaggatcatgggaggaaagaggatccttattatcttggacgacatttgggatatagtagatatggcaaacattggaattcctagctatgacgagcttcaaagatgcaattccaaggtcctactcaccacaaggatcaggaatgtttgttattctatggggtgccaagcacatgttcctctgaatatcctatcagaacaagattctttgaagttagtcatgaagaagtcgagaaagccttttcatgaatctactgattcctatgatgaagagaggaacatagttaaagaatgcgctggtctcccaaatttattggaagaagctgcaagggcacttggacataaatacatggaagaatggaacgaagcagtttcaaaggcattcggagattatgacaatgaattgcaaaatgcagctcaagaactaaatgcatctcaacttgtccacactaaagatgagagagatacatttagaagaataaagttaagctatcataccttgaggtttgatgatgccaagtcatttttcttgctttgctgcatgtttccagaagattatgatatccgagttgaagaattgttgaagtatgggattggaaaaggtctgcttcaaaattcagacacattgcaagaagctagagacagagcacattttttggtcacataccttaaagcttcggggttgcttttggatggcaagaagggatgcataaggatgcattgtgacatccgaaatgtggccatgtctattgcattgtctgaaggtcatgcttttttcgtaaaagcgggttgtgaattgaaggattggccaatgtatgctcatgaaggctactcagcaatctcactaatgaggaacaaacttaacaagcttcctaaaactttggtatgttctgaactccaaattttactgctgcaaagtaatgctggtatatgtgacattccaaaaactttttttcaatgtccaaatgcactaagggtctttgatcttagctataccggcatttcagtactaccctcttcattcaatctcctcaccaatctccaatccttgtatttggattgttgcaataaaataactgacatctctgtacttgggaagctgaagaaagttgagattcttagtttgaaaaaatttcctctcaaggagttgccgaaagagataggaaatttgaccagtctaaggatgctggacgtcactggtggatatgttcacaaaattccatgtaaagtgctatcgaggttgcatagactggaagaattatacatgaaatgtgaatttggggactggggaagtaaagtcgaggaagcaggacaaacaaatgccgactttaatgagataattgggttattccgtttaaacattttgaaggtttacatatccgatgcagcttgcttacccaaagaaattgggtactttgagccgacttgggtaaaatttgacatatgcataagcagagatctctcttcggaaatgatgcttgctacgaatgatcaaccttcaagaaccttgactcttgacagaacaatcaatggcttatcggattggtttatcaatgtggtgacacagaaagcagaaaaactagagtacagagagtgcaagggattaaacgacattcttaaggaatatgagcatgggagattacatgggctcaagcatctctctgtaattgggtgccatgaaaacttggaagagttgataaatgcaacaacatgggatccaagtaaacctgtgttcgagagcttggaagagctgcatctccttcacttgcaacgcctcaaggagttatgtgttggcgaattaccactgggatctctgtgcaatctcaagctattgaaagtgcaaacttgttgtaacttggtgaatgcacttttaccaccaaaattgttgcagagactaataaacctagaaacaatattttgtgtaaatatggaacacatgaaatatttgtttggctctgaagggctggagcttggaacgactctcttgacaaaattgagcgaggtgagactgtgcgctctcttcgaactaataagcatctggaatggtccggcaccaaatgcaatctttgggaatcttaaagttttggcagtttgcaagtgtacaaaactgaaacatctattcccattcgatgtagctcgatctcttgtgcaattggaagacctctctgtagagcgctgccctaacttggacagagttattgaagcatgcaacagcttgaacagatatatctttttcccagaattgaagaaattagctttgatagatcttccagagctcacatggttctgcaccgaaaatggtactgttcatatcgagtgccctctgttggaatacttgtatgtggatgagtgcccccagtttgtaaactctgcttctgcttctgttaaactcaacgatcaacaacatttgaattctctagtgataaggtatgtctctgcgttataa

Protein Analysis

1551

Amino Acids

176.79

Weight (kDa)

6.18

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4654
AatII GACGTC 1 cut(s) 3405
Acc16I TGCGCA 1 cut(s) 560
AccB1I GGYRCC 3 cut(s) 2373, 3882, 4261
AccI GTMKAC 2 cut(s) 363, 966
AccII CGCG 1 cut(s) 156
AciI CCGC 1 cut(s) 2996
AcoI YGGCCR 5 cut(s) 210, 331, 934, 2949, 3017
AcuI CTGAAG 5 cut(s) 1362, 1408, 2991, 3332, 4188
AcyI GRCGYC 1 cut(s) 3402
AfaI GTAC 8 cut(s) 1710, 1902, 3219, 3300, 3623, 3797, 4312, 4513
AfeI AGCGCT 1 cut(s) 4385
AfiI CCNNNNNNNGG 5 cut(s) 666, 2947, 3390, 4396, 4537
AflII CTTAAG 1 cut(s) 3827
AflIII ACRYGT 2 cut(s) 2047, 2383
AjnI CCWGG 2 cut(s) 5, 23
AjuI GAANNNNNNNTTGG 4 cut(s) 2370, 2402, 2720, 2752
Alw21I GWGCWC 4 cut(s) 1893, 2059, 2860, 4488
Alw26I GTCTC 6 cut(s) 164, 2516, 2844, 4096, 4210, 4648
Alw44I GTGCAC 1 cut(s) 2055
Aor51HI AGCGCT 1 cut(s) 4385
AoxI GGCC 7 cut(s) 3, 210, 331, 934, 1737, 2949, 3017
ApaLI GTGCAC 1 cut(s) 2055
ApeKI GCWGC 9 cut(s) 739, 790, 2534, 2624, 2748, 3121, 3596, 3961, 4386
AseI ATTAAT 1 cut(s) 188
Asp700I GAANNNNTTC 1 cut(s) 1902
AspLEI GCGC 4 cut(s) 561, 2507, 4226, 4386
AspS9I GGNCC 5 cut(s) 426, 451, 1054, 2329, 4256
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 9 cut(s) 403, 1006, 1442, 2053, 2329, 3235, 3781, 4081, 4225
AsuII TTCGAA 1 cut(s) 4233
AvaII GGWCC 5 cut(s) 426, 451, 1054, 2329, 4256
BaeGI GKGCMC 4 cut(s) 2059, 2545, 4532, 4565
BalI TGGCCA 3 cut(s) 212, 2951, 3019
BamHI GGATCC 3 cut(s) 1696, 2235, 3925
BanI GGYRCC 3 cut(s) 2373, 3882, 4261
BanII GRGCYC 2 cut(s) 3861, 4488
BarI GAAGNNNNNNTAC 4 cut(s) 124, 156, 700, 732
BbsI GAAGAC 2 cut(s) 1878, 4374
Bbv12I GWGCWC 4 cut(s) 1893, 2059, 2860, 4488
BbvI GCAGC 9 cut(s) 726, 777, 2521, 2636, 2735, 3108, 3608, 3948, 4373
BccI CCATC 5 cut(s) 79, 194, 655, 1154, 2897
BceAI ACGGC 3 cut(s) 346, 949, 1572
BciT130I CCWGG 2 cut(s) 7, 25
BciVI GTATCC 1 cut(s) 1660
BclI TGATCA 1 cut(s) 3700
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 6 cut(s) 164, 2516, 2844, 4096, 4210, 4648
BfaI CTAG 8 cut(s) 369, 972, 1172, 2295, 2847, 3791, 4115, 4628
BfmI CTRYAG 1 cut(s) 4377
BfoI RGCGCY 1 cut(s) 4387
BfrI CTTAAG 1 cut(s) 3827
BfuI GTATCC 1 cut(s) 1660
BglII AGATCT 2 cut(s) 3667, 4474
BisI GCNGC 9 cut(s) 740, 791, 2535, 2625, 2749, 3122, 3597, 3962, 4387
BlsI GCNGC 9 cut(s) 741, 792, 2536, 2626, 2750, 3123, 3598, 3963, 4388
BmcAI AGTACT 2 cut(s) 1902, 3219
Bme1390I CCNGG 3 cut(s) 7, 25, 430
Bme18I GGWCC 5 cut(s) 426, 451, 1054, 2329, 4256
BmgT120I GGNCC 5 cut(s) 426, 451, 1054, 2329, 4256
BmiI GGNNCC 6 cut(s) 1698, 2237, 2375, 3884, 3927, 4263
BmrFI CCNGG 3 cut(s) 7, 25, 430
BmrI ACTGGG 3 cut(s) 3505, 4025, 4561
BmuI ACTGGG 3 cut(s) 3505, 4025, 4561
BpiI GAAGAC 2 cut(s) 1878, 4374
BpmI CTGGAG 1 cut(s) 4196
Bpu14I TTCGAA 1 cut(s) 4233
BpuEI CTTGAG 9 cut(s) 493, 1505, 1527, 2613, 2731, 3335, 3845, 3971, 4019
BpuMI CCSGG 1 cut(s) 430
BsaBI GATNNNNATC 2 cut(s) 439, 1042
BsaHI GRCGYC 1 cut(s) 3402
BsaI GGTCTC 1 cut(s) 2516
BsaJI CCNNGG 5 cut(s) 661, 1913, 1953, 2037, 2325
Bsc4I CCNNNNNNNGG 5 cut(s) 666, 2947, 3390, 4396, 4537
Bse118I RCCGGY 1 cut(s) 3206
Bse3DI GCAATG 1 cut(s) 2834
Bse8I GATNNNNATC 2 cut(s) 439, 1042
BseBI CCWGG 2 cut(s) 7, 25
BseDI CCNNGG 5 cut(s) 661, 1913, 1953, 2037, 2325
BseJI GATNNNNATC 2 cut(s) 439, 1042
BseLI CCNNNNNNNGG 5 cut(s) 666, 2947, 3390, 4396, 4537
BseMI GCAATG 1 cut(s) 2834
BseMII CTCAG 4 cut(s) 33, 1409, 1845, 3057
BseRI GAGGAG 3 cut(s) 1314, 1383, 3230
BseSI GKGCMC 4 cut(s) 2059, 2545, 4532, 4565
BseXI GCAGC 9 cut(s) 726, 777, 2521, 2636, 2735, 3108, 3608, 3948, 4373
BseYI CCCAGC 1 cut(s) 2155
BsgI GTGCAG 2 cut(s) 1183, 4483
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 7 cut(s) 5, 212, 333, 936, 1739, 2951, 3019
BshNI GGYRCC 3 cut(s) 2373, 3882, 4261
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 4 cut(s) 1893, 2059, 2860, 4488
BsiSI CCGG 3 cut(s) 429, 3207, 4259
BslFI GGGAC 1 cut(s) 3506
BslI CCNNNNNNNGG 5 cut(s) 666, 2947, 3390, 4396, 4537
BsmAI GTCTC 6 cut(s) 164, 2516, 2844, 4096, 4210, 4648
BsmFI GGGAC 1 cut(s) 3506
BsmI GAATGC 5 cut(s) 781, 1750, 2507, 2591, 4078
BsnI GGCC 7 cut(s) 5, 212, 333, 936, 1739, 2951, 3019
Bso31I GGTCTC 1 cut(s) 2516
Bsp119I TTCGAA 1 cut(s) 4233
Bsp1286I GDGCHC 8 cut(s) 1893, 2059, 2545, 2860, 3861, 4488, 4532, 4565
Bsp1407I TGTACA 1 cut(s) 4310
Bsp19I CCATGG 2 cut(s) 1953, 2037
BspACI CCGC 1 cut(s) 2996
BspANI GGCC 7 cut(s) 5, 212, 333, 936, 1739, 2951, 3019
BspCNI CTCAG 4 cut(s) 34, 1408, 1846, 3056
BspFNI CGCG 1 cut(s) 156
BspHI TCATGA 3 cut(s) 2430, 2455, 3031
BspLI GGNNCC 6 cut(s) 1698, 2237, 2375, 3884, 3927, 4263
BspQI GCTCTTC 1 cut(s) 3951
BspT104I TTCGAA 1 cut(s) 4233
BspT107I GGYRCC 3 cut(s) 2373, 3882, 4261
BspTI CTTAAG 1 cut(s) 3827
BspTNI GGTCTC 1 cut(s) 2516
BsrDI GCAATG 1 cut(s) 2834
BsrFI RCCGGY 1 cut(s) 3206
BsrGI TGTACA 1 cut(s) 4310
BssAI RCCGGY 1 cut(s) 3206
BssECI CCNNGG 5 cut(s) 661, 1913, 1953, 2037, 2325
BssNI GRCGYC 1 cut(s) 3402
BssT1I CCWWGG 4 cut(s) 1913, 1953, 2037, 2325
Bst2UI CCWGG 2 cut(s) 7, 25
Bst4CI ACNGT 3 cut(s) 1111, 4221, 4516
Bst6I CTCTTC 9 cut(s) 57, 162, 2008, 2475, 3232, 3679, 3894, 3951, 4235
BstACI GRCGYC 1 cut(s) 3402
BstAFI CTTAAG 1 cut(s) 3827
BstAPI GCANNNNNTGC 1 cut(s) 4268
BstAUI TGTACA 1 cut(s) 4310
BstBI TTCGAA 1 cut(s) 4233
BstC8I GCNNGC 3 cut(s) 3601, 3690, 4417
BstDEI CTNAG 8 cut(s) 42, 1395, 1854, 3043, 3182, 3197, 3329, 3389
BstDSI CCRYGG 3 cut(s) 661, 1953, 2037
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 4387
BstHHI GCGC 4 cut(s) 561, 2507, 4226, 4386
BstMAI GTCTC 6 cut(s) 164, 2516, 2844, 4096, 4210, 4648
BstMCI CGRYCG 1 cut(s) 160
BstNI CCWGG 2 cut(s) 7, 25
BstNSI RCATGY 4 cut(s) 2051, 2387, 2755, 4419
BstSCI CCNGG 3 cut(s) 5, 23, 428
BstSFI CTRYAG 1 cut(s) 4377
BstSLI GKGCMC 4 cut(s) 2059, 2545, 4532, 4565
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 9 cut(s) 726, 777, 2521, 2636, 2735, 3108, 3608, 3948, 4373
BstV2I GAAGAC 2 cut(s) 1878, 4374
BstX2I RGATCY 6 cut(s) 1696, 2235, 3667, 3925, 4019, 4474
BstYI RGATCY 6 cut(s) 1696, 2235, 3667, 3925, 4019, 4474
BsuI GTATCC 1 cut(s) 1660
BsuRI GGCC 7 cut(s) 5, 212, 333, 936, 1739, 2951, 3019
BtgI CCRYGG 3 cut(s) 661, 1953, 2037
BtsI GCAGTG 4 cut(s) 464, 1067, 1796, 1992
Cac8I GCNNGC 3 cut(s) 3601, 3690, 4417
CciI TCATGA 3 cut(s) 2430, 2455, 3031
CfoI GCGC 4 cut(s) 561, 2507, 4226, 4386
Cfr10I RCCGGY 1 cut(s) 3206
Cfr13I GGNCC 5 cut(s) 426, 451, 1054, 2329, 4256
Csp6I GTAC 8 cut(s) 1709, 1901, 3218, 3299, 3622, 3796, 4311, 4512
CspCI CAANNNNNGTGG 2 cut(s) 4074, 4109
CviQI GTAC 8 cut(s) 1709, 1901, 3218, 3299, 3622, 3796, 4311, 4512
DdeI CTNAG 8 cut(s) 42, 1395, 1854, 3043, 3182, 3197, 3329, 3389
DraI TTTAAA 2 cut(s) 1806, 3567
EaeI YGGCCR 5 cut(s) 210, 331, 934, 2949, 3017
Eam1104I CTCTTC 9 cut(s) 57, 162, 2008, 2475, 3232, 3679, 3894, 3951, 4235
EarI CTCTTC 9 cut(s) 57, 162, 2008, 2475, 3232, 3679, 3894, 3951, 4235
Ecl136II GAGCTC 1 cut(s) 4486
Eco130I CCWWGG 4 cut(s) 1913, 1953, 2037, 2325
Eco24I GRGCYC 2 cut(s) 3861, 4488
Eco31I GGTCTC 1 cut(s) 2516
Eco32I GATATC 2 cut(s) 1715, 2773
Eco47I GGWCC 5 cut(s) 426, 451, 1054, 2329, 4256
Eco47III AGCGCT 1 cut(s) 4385
Eco53kI GAGCTC 1 cut(s) 4486
Eco57I CTGAAG 5 cut(s) 1362, 1408, 2991, 3332, 4188
EcoICRI GAGCTC 1 cut(s) 4486
EcoO109I RGGNCCY 1 cut(s) 2329
EcoRI GAATTC 4 cut(s) 339, 942, 2289, 4621
EcoRII CCWGG 2 cut(s) 5, 23
EcoRV GATATC 2 cut(s) 1715, 2773
EcoT14I CCWWGG 4 cut(s) 1913, 1953, 2037, 2325
EcoT22I ATGCAT 6 cut(s) 1655, 2143, 2644, 2921, 2931, 3659
EcoT38I GRGCYC 2 cut(s) 3861, 4488
ErhI CCWWGG 4 cut(s) 1913, 1953, 2037, 2325
FalI AAGNNNNNCTT 4 cut(s) 1887, 1919, 2802, 2834
FaqI GGGAC 1 cut(s) 3506
FauI CCCGC 1 cut(s) 2989
FauNDI CATATG 1 cut(s) 3653
FbaI TGATCA 1 cut(s) 3700
FblI GTMKAC 2 cut(s) 363, 966
Fnu4HI GCNGC 9 cut(s) 740, 791, 2535, 2625, 2749, 3122, 3597, 3962, 4387
FriOI GRGCYC 2 cut(s) 3861, 4488
Fsp4HI GCNGC 9 cut(s) 740, 791, 2535, 2625, 2749, 3122, 3597, 3962, 4387
FspBI CTAG 8 cut(s) 369, 972, 1172, 2295, 2847, 3791, 4115, 4628
FspI TGCGCA 1 cut(s) 560
GlaI GCGC 4 cut(s) 560, 2506, 4225, 4385
GluI GCNGC 9 cut(s) 740, 791, 2535, 2625, 2749, 3122, 3597, 3962, 4387
GsaI CCCAGC 1 cut(s) 2159
GsuI CTGGAG 1 cut(s) 4196
HaeII RGCGCY 1 cut(s) 4387
HaeIII GGCC 7 cut(s) 5, 212, 333, 936, 1739, 2951, 3019
HapII CCGG 3 cut(s) 429, 3207, 4259
HhaI GCGC 4 cut(s) 561, 2507, 4226, 4386
Hin1I GRCGYC 1 cut(s) 3402
Hin6I GCGC 4 cut(s) 559, 2505, 4224, 4384
HinP1I GCGC 4 cut(s) 559, 2505, 4224, 4384
HincII GTYRAC 3 cut(s) 364, 967, 1338
HindII GTYRAC 3 cut(s) 364, 967, 1338
HindIII AAGCTT 2 cut(s) 2883, 3077
HpaII CCGG 3 cut(s) 429, 3207, 4259
HphI GGTGA 9 cut(s) 403, 1006, 1442, 2053, 2329, 3235, 3781, 4081, 4225
HpyCH4III ACNGT 3 cut(s) 1111, 4221, 4516
HpyCH4IV ACGT 1 cut(s) 3402
HpyF3I CTNAG 8 cut(s) 42, 1395, 1854, 3043, 3182, 3197, 3329, 3389
HpySE526I ACGT 1 cut(s) 3402
Hsp92I GRCGYC 1 cut(s) 3402
HspAI GCGC 4 cut(s) 559, 2505, 4224, 4384
Ksp22I TGATCA 1 cut(s) 3700
LguI GCTCTTC 1 cut(s) 3951
LmnI GCTCC 3 cut(s) 1301, 1396, 4177
Lsp1109I GCAGC 9 cut(s) 726, 777, 2521, 2636, 2735, 3108, 3608, 3948, 4373
MaeI CTAG 8 cut(s) 369, 972, 1172, 2295, 2847, 3791, 4115, 4628
MaeII ACGT 1 cut(s) 3402
MaeIII GTNAC 6 cut(s) 2869, 2933, 3143, 3403, 3769, 4061
MfeI CAATTG 6 cut(s) 228, 733, 831, 852, 1560, 4361
MflI RGATCY 6 cut(s) 1696, 2235, 3667, 3925, 4019, 4474
MhlI GDGCHC 8 cut(s) 1893, 2059, 2545, 2860, 3861, 4488, 4532, 4565
MlsI TGGCCA 3 cut(s) 212, 2951, 3019
MluNI TGGCCA 3 cut(s) 212, 2951, 3019
MlyI GAGTC 4 cut(s) 1271, 2013, 3715, 4183
MmeI TCCRAC 2 cut(s) 1239, 4518
Mox20I TGGCCA 3 cut(s) 212, 2951, 3019
Mph1103I ATGCAT 6 cut(s) 1655, 2143, 2644, 2921, 2931, 3659
MroXI GAANNNNTTC 1 cut(s) 1902
MscI TGGCCA 3 cut(s) 212, 2951, 3019
MslI CAYNNNNRTG 2 cut(s) 2052, 2924
Msp20I TGGCCA 3 cut(s) 212, 2951, 3019
MspA1I CMGCKG 2 cut(s) 65, 2155
MspCI CTTAAG 1 cut(s) 3827
MspI CCGG 3 cut(s) 429, 3207, 4259
MspR9I CCNGG 3 cut(s) 7, 25, 430
MssI GTTTAAAC 1 cut(s) 3567
MunI CAATTG 6 cut(s) 228, 733, 831, 852, 1560, 4361
Mva1269I GAATGC 5 cut(s) 781, 1750, 2507, 2591, 4078
MvaI CCWGG 2 cut(s) 7, 25
MvnI CGCG 1 cut(s) 156
NciI CCSGG 1 cut(s) 430
NcoI CCATGG 2 cut(s) 1953, 2037
NdeI CATATG 1 cut(s) 3653
NlaIV GGNNCC 6 cut(s) 1698, 2237, 2375, 3884, 3927, 4263
NmuCI GTSAC 5 cut(s) 2869, 2933, 3143, 3403, 3769
NsbI TGCGCA 1 cut(s) 560
NsiI ATGCAT 6 cut(s) 1655, 2143, 2644, 2921, 2931, 3659
NspI RCATGY 4 cut(s) 2051, 2387, 2755, 4419
NspV TTCGAA 1 cut(s) 4233
PaeI GCATGC 1 cut(s) 4419
PagI TCATGA 3 cut(s) 2430, 2455, 3031
PciI ACATGT 2 cut(s) 2047, 2383
PciSI GCTCTTC 1 cut(s) 3951
PctI GAATGC 5 cut(s) 781, 1750, 2507, 2591, 4078
PdmI GAANNNNTTC 1 cut(s) 1902
PkrI GCNGC 9 cut(s) 741, 792, 2536, 2626, 2750, 3123, 3598, 3963, 4388
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 4 cut(s) 1270, 2012, 3715, 4183
PmeI GTTTAAAC 1 cut(s) 3567
PpsI GAGTC 4 cut(s) 1270, 2012, 3715, 4183
PpuMI RGGWCCY 1 cut(s) 2329
PscI ACATGT 2 cut(s) 2047, 2383
PshBI ATTAAT 1 cut(s) 188
PsiI TTATAA 1 cut(s) 4654
Psp124BI GAGCTC 1 cut(s) 4488
Psp5II RGGWCCY 1 cut(s) 2329
Psp6I CCWGG 2 cut(s) 5, 23
PspFI CCCAGC 1 cut(s) 2155
PspGI CCWGG 2 cut(s) 5, 23
PspN4I GGNNCC 6 cut(s) 1698, 2237, 2375, 3884, 3927, 4263
PspPI GGNCC 5 cut(s) 426, 451, 1054, 2329, 4256
PspPPI RGGWCCY 1 cut(s) 2329
PsuI RGATCY 6 cut(s) 1696, 2235, 3667, 3925, 4019, 4474
PvuI CGATCG 1 cut(s) 160
PvuII CAGCTG 2 cut(s) 65, 2155
RsaI GTAC 8 cut(s) 1710, 1902, 3219, 3300, 3623, 3797, 4312, 4513
RsaNI GTAC 8 cut(s) 1709, 1901, 3218, 3299, 3622, 3796, 4311, 4512
RseI CAYNNNNRTG 2 cut(s) 2052, 2924
SacI GAGCTC 1 cut(s) 4488
SalI GTCGAC 2 cut(s) 362, 965
SapI GCTCTTC 1 cut(s) 3951
SatI GCNGC 9 cut(s) 740, 791, 2535, 2625, 2749, 3122, 3597, 3962, 4387
Sau96I GGNCC 5 cut(s) 426, 451, 1054, 2329, 4256
ScaI AGTACT 2 cut(s) 1902, 3219
SchI GAGTC 4 cut(s) 1271, 2013, 3715, 4183
ScrFI CCNGG 3 cut(s) 7, 25, 430
SduI GDGCHC 8 cut(s) 1893, 2059, 2545, 2860, 3861, 4488, 4532, 4565
SfcI CTRYAG 1 cut(s) 4377
SfuI TTCGAA 1 cut(s) 4233
SinI GGWCC 5 cut(s) 426, 451, 1054, 2329, 4256
SmiMI CAYNNNNRTG 2 cut(s) 2052, 2924
SphI GCATGC 1 cut(s) 4419
SsiI CCGC 1 cut(s) 2996
SspI AATATT 2 cut(s) 4125, 4154
SspMI CTAG 8 cut(s) 369, 972, 1172, 2295, 2847, 3791, 4115, 4628
SstI GAGCTC 1 cut(s) 4488
StyD4I CCNGG 3 cut(s) 5, 23, 428
StyI CCWWGG 4 cut(s) 1913, 1953, 2037, 2325
TaaI ACNGT 3 cut(s) 1111, 4221, 4516
TaiI ACGT 1 cut(s) 3405
TatI WGTACW 5 cut(s) 1900, 3217, 3298, 3795, 4310
TseFI GTSAC 5 cut(s) 2869, 2933, 3143, 3403, 3769
TseI GCWGC 9 cut(s) 739, 790, 2534, 2624, 2748, 3121, 3596, 3961, 4386
Tsp45I GTSAC 5 cut(s) 2869, 2933, 3143, 3403, 3769
TspGWI ACGGA 4 cut(s) 338, 453, 1056, 3551
Vha464I CTTAAG 1 cut(s) 3827
VneI GTGCAC 1 cut(s) 2055
VpaK11BI GGWCC 5 cut(s) 426, 451, 1054, 2329, 4256
VspI ATTAAT 1 cut(s) 188
XceI RCATGY 4 cut(s) 2051, 2387, 2755, 4419
XcmI CCANNNNNNNNNTGG 2 cut(s) 1395, 4488
XmiI GTMKAC 2 cut(s) 363, 966
XmnI GAANNNNTTC 1 cut(s) 1902
XspI CTAG 8 cut(s) 369, 972, 1172, 2295, 2847, 3791, 4115, 4628
ZraI GACGTC 1 cut(s) 3403
ZrmI AGTACT 2 cut(s) 1902, 3219
Zsp2I ATGCAT 6 cut(s) 1655, 2143, 2644, 2921, 2931, 3659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.