FvH4_7g31110

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000085
Physical Location & Seq
Forward (+)
22509679 .. 22514328
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31110.t1

Sequence Viewer

Length: 3306 bp
ATGGCCTCGAGCTCCCAAACAGCTTCTGCTTCTTTTCCTTCGTCAGCTCCTAGTTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTGTAGATACTCGCAAGGGTATTACGTTCGAGTTATACGATCGTCTGCATAACGGAAGAGGAATTAAAACATTCATGGATGGCCAAGATCTTGACGTAGGAGCTCTTATTTCTCCAAGACTCTTCAAGGCAATCGAAGAATCAATGGTTGCAATTGTTGTTCTCTCCTCAAATTATGCTTCTTCGAGTTGGTGTTTGGAGGAACTTACCAAAATTTGTCAATGTATGCAAGATGATGATAGAATTCTTCCAGTTTTCTATCATGTAGAACCCTCTGATGTACGCTATCAAAGGAAGAGTTTCGAAGAAGCTTTCACTAAGCATGAAAACTGTGGGCGACAAAAAATGGAGATGCAGCAGTGGAGAGATGCTTTAAATAGGGTGGCCAATTTTTGTGGGTGGCATACACAAAATTACAAGACTGAAAGAGAACTTGTCCACGCCATTGTGAAATCAGTGTGCAGTAAAGTACGACCTATCAAAATTGATTTTACAATGTCCAATGGAGATTTTAAAGCATTTGAAGCAACAAAACGAGCTATGAATAAGGTCATGAATGAGCTAAATGATGATGAGGTCACTGTGATTGGGGTCTACGGAATGGGAGGCGTTGGAAAGACAACCATGGTAAAACACGTCGGTGAACAAATGAAGAAAAGTAGACTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCTGATTTGAGGAAAATTCAAGGCACATTAGCAGATTTGCTGGACTTGAAATTGGAGGATGAGACAGAAATAGGAAGAGCCTGTAGATTGGAGGAGGAGATAAAAAGAGGAACCAAGATCCTCATAATCTTGGATGATGTTTGGAATAGAATAGAACTTTCAAGCATAGGAATTCCCAGCCACGACAAACTTCAAAGACGCAATTCCAAAGTCCTTCTCACCACAAGGAGATTGTCTGTATGTCATTTCATGAACAGCCATGCTAACATTCCGCTTGATATCTTATCAGAAGAAGATGCTTGGAACTTGTTTGTCACAGAAGCAAGACAATGTTTTGACAGATCCACCAGTTTTTATGATGTGGCGAGGATGGTGGCTAGAGAATGCGCTGGCCTACCAGTTGCGTTGATAGCAGTTGCGAGGGCACTTCGATATGCAAGTTTTAATGAATGGAAACACTCAGCTCAACGTCTAAAGGCATCTCAACCTGCCACCCCTGAAGATGAGGGAGGTGTGTACAAATGCATAAAATTAAGCTATGATTACTTAAAATCTGATGAATCCAAATCATTCTTCTTGCTTTGTTGTCTGTTCCCGGATGACTACGACATCTCAGTTGATCAGTTGCTGAAGTATGGAATTGGGAAAGGGATGTTTCAAGATTCCAACATGCAAGAAGCCAGAGCCACAGCACATTTAGTGGTCAAGTCCCTTCAAGCTTCTAGCTTGCTTTTGGACTCTAAGAAGGATGGATGCGTAAGGATGCATGATGTCATTCGGGATGTGGCCATTTTAATTTCAATATCTGAAGTTGGTAACCAGGTTTTTGTGAAAGCTAGTCATGACTTGAAGCATTGGCCAATGATTAATGCGCACAAAGGCTACTCTGCCATCTCAGTAATGAAGAACCAAATTGGAAAACTACCTAAAGAGTTGGTATGTCCAAACCTCAGAATTTTACTGCTTCAACATAATCCAAATTTAAATGAGATACCCGAGACTTTTCTTCATACCCTGAATGAACTAGGAGTCTTGGATCTTAGCTACACTAGTATTTCATTACTACCCCAATCACTTAGTCTCCTAACCAACCTTCAAGCTTTGTATTTAGATTGTTGCAAGAAAATAATTGACTTTTCCATACTCGGAAAACTTGAGAAGCTTGAGATTCTTAGTATGAGAGAATACCCTCTCAAGGAATTGTCAAGGGAAATAGGACGCTTGACCAATTTAAGAATGCTGGATGTCAGTGATGGTTCATGGCATGGACATATTGTTACAATTCCAACTAAAGTGATATCAAAGATGCATAAGCTAGAAGAACTGTACATGCATTGCAGATTTGGAGAGTGGGGGAGTAAAGTCAAGGAAGAAGGAGAAGAGACTAATATTGGATTCGACGAGTTAACTGGCTTATCATACTTGTACATCTTGACGGTTCGCATATGCGATGTAAAATGTTTGCCTAAATGTGTTGAGTTCATTCCAAACTGGATCTACTTTGATATCCGTTTCGGTGGAGACAGAAGAGCACCTCATATTCAGTCTCAGATTGGTCATGAATATTCCAGATCCTTGCTTCTTAACACCACTATCTGCACCTTACCAGATTGGTTTGTTAGCGTGGTGACAAAGAAAACGGAGAATCTACAGTTTGCAAAGTGCGAAGGCTTAAATAACATTCTTGTGGAATATGACCGTGGGAAGTTATGCGAGCTAAAGCATCTCTCTATTATTGGCCCATGTGAGAAGTTGAAAGAGCTGATGAACATAACAAGTTGGTATTCACATAAACCTGTATTCGAGAACTTGGAGGAGTTGCATTTGGATGGTGTGAAATCCCTAACTAAATTATGTGTTGGTGAATTACCGATAGGATCTTTGTTTAATCTTAAGCTGTTGAATGTCTGCAATTGTCGTAACTTGGGGGATGTACTGTTGTCATCAAAACTGCTGCGGAGACTGCCAAATCTGGAAAAGGTGATTTGCAATGTTATGATTAGCATGGAATATGTATTCGGATACGAAGGGTTTGAGCCAGAAGAATCAAAGCTCAGAGAGATGACAATGTTGAGTCTGTATGCTCTAAGAAGCATATGTAATGGTCCTGCTCCACGGACAATGTTCCAGACTCTGAAAAGTTTGCTCATTTACCATTGTGATCAGCTTGGAGGAAGCCTCTTCACATATGATGTAGCTCAGTGCCTTTTTCATCTGGAAGACCTTTTTATATTCAGTTGCCCTCTCTTGAAAAATTTGATTGAAGCAAGCGAGGAAACAGTGAGCAAGAAGAAGATCGTTCTTCCAAAAATGAAGAGCTTAGTTTTGCTGATGCTTCCAAGACTGTCTAGCGAAAATGCTATTCATATTGAGTCCCCTTCATTGATGCATTTATATATCCAATATTGCCCCAACTTCTTGAATGATGCTATTATTTTCCACAACAAGAACCAAGTCCAACTGAATGATGAACTACAAATGAGGTTTCTATGGTCGAGATTGATGAATAACATACCGTAG

Protein Analysis

1102

Amino Acids

125.69

Weight (kDa)

7.0

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 186 2e-47 TIR domain
TIR_2 PF13676 23 - 119 1.8e-12 TIR domain
NB-ARC PF00931 207 - 370 2.4e-30 NB-ARC domain
WHD_DRP PF23559 459 - 525 6.2e-06 Disease resistance protein Winged helix domain
LRR_8 PF13855 577 - 633 9.2e-07 Leucine rich repeat
LRR_14 PF23598 578 - 765 9.8e-17 Leucine-rich repeat region
LRR_RPS2 PF23247 866 - 1009 5e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 949 - 1067 1.6e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1659
Acc36I ACCTGC 1 cut(s) 1282
AccI GTMKAC 2 cut(s) 678, 745
AciI CCGC 2 cut(s) 1058, 2743
AclWI GGATC 6 cut(s) 898, 1122, 1830, 2288, 2352, 2671
AcoI YGGCCR 4 cut(s) 166, 468, 1572, 1643
AcsI RAATTY 7 cut(s) 297, 327, 801, 957, 1740, 1765, 3040
AcuI CTGAAG 3 cut(s) 1305, 1436, 1614
AfaI GTAC 6 cut(s) 366, 555, 1304, 2114, 2213, 2721
AfiI CCNNNNNNNGG 1 cut(s) 1981
AflII CTTAAG 1 cut(s) 2678
AflIII ACRYGT 1 cut(s) 718
AhlI ACTAGT 1 cut(s) 1835
AjiI CACGTC 1 cut(s) 721
AjnI CCWGG 1 cut(s) 1605
AjuI GAANNNNNNNTTGG 2 cut(s) 1343, 1375
AleI CACNNNNGTG 1 cut(s) 723
Alw21I GWGCWC 3 cut(s) 14, 190, 2320
Alw26I GTCTC 7 cut(s) 842, 1778, 1871, 2162, 2301, 2337, 2740
AlwI GGATC 6 cut(s) 898, 1122, 1830, 2288, 2352, 2671
AlwNI CAGNNNCTG 3 cut(s) 26, 1414, 2920
Ama87I CYCGRG 2 cut(s) 7, 1781
AoxI GGCC 7 cut(s) 3, 166, 468, 1177, 1572, 1643, 2524
ApeKI GCWGC 2 cut(s) 439, 2740
ApoI RAATTY 7 cut(s) 297, 327, 801, 957, 1740, 1765, 3040
AseI ATTAAT 1 cut(s) 1653
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 2 cut(s) 1175, 1660
AspS9I GGNCC 2 cut(s) 2525, 2891
AsuC2I CCSGG 1 cut(s) 1382
AsuHPI GGTGA 5 cut(s) 737, 997, 2425, 2660, 2779
AsuII TTCGAA 1 cut(s) 387
AvaI CYCGRG 2 cut(s) 7, 1781
AvaII GGWCC 1 cut(s) 2891
BaeGI GKGCMC 1 cut(s) 1213
BalI TGGCCA 4 cut(s) 168, 470, 1574, 1645
BanII GRGCYC 2 cut(s) 14, 190
BbsI GAAGAC 1 cut(s) 3012
Bbv12I GWGCWC 3 cut(s) 14, 190, 2320
BbvI GCAGC 2 cut(s) 451, 2727
BccI CCATC 6 cut(s) 158, 1150, 1529, 1685, 2033, 2609
BciT130I CCWGG 1 cut(s) 1607
BciVI GTATCC 2 cut(s) 787, 2801
BclI TGATCA 2 cut(s) 1405, 2947
BcnI CCSGG 1 cut(s) 1382
BcoDI GTCTC 7 cut(s) 842, 1778, 1871, 2162, 2301, 2337, 2740
BcuI ACTAGT 1 cut(s) 1835
BfaI CTAG 8 cut(s) 51, 1164, 1509, 1623, 1811, 1836, 2102, 3135
BfmI CTRYAG 2 cut(s) 868, 2435
BfrI CTTAAG 1 cut(s) 2678
BfuAI ACCTGC 1 cut(s) 1282
BfuI GTATCC 2 cut(s) 787, 2801
BglII AGATCT 1 cut(s) 172
BisI GCNGC 2 cut(s) 440, 2741
BlsI GCNGC 2 cut(s) 441, 2742
Bme1390I CCNGG 2 cut(s) 1382, 1607
Bme18I GGWCC 1 cut(s) 2891
BmeT110I CYCGRG 2 cut(s) 7, 1781
BmgBI CACGTC 1 cut(s) 721
BmgT120I GGNCC 2 cut(s) 2525, 2891
BmiI GGNNCC 1 cut(s) 898
BmrFI CCNGG 2 cut(s) 1382, 1607
BpiI GAAGAC 1 cut(s) 3012
BplI GAGNNNNNCTC 2 cut(s) 2949, 2981
Bpu14I TTCGAA 1 cut(s) 387
BpuEI CTTGAG 3 cut(s) 1961, 1964, 1970
BpuMI CCSGG 1 cut(s) 1382
BsaJI CCNNGG 3 cut(s) 708, 2485, 2900
BsaXI ACNNNNNCTCC 2 cut(s) 1851, 1881
Bsc4I CCNNNNNNNGG 1 cut(s) 1981
Bse1I ACTGG 5 cut(s) 335, 1134, 1184, 2200, 2282
Bse3DI GCAATG 2 cut(s) 2119, 2782
BseBI CCWGG 1 cut(s) 1607
BseDI CCNNGG 3 cut(s) 708, 2485, 2900
BseLI CCNNNNNNNGG 1 cut(s) 1981
BseMI GCAATG 2 cut(s) 2119, 2782
BseMII CTCAG 7 cut(s) 1260, 1413, 1695, 1750, 2348, 2854, 2999
BseNI ACTGG 5 cut(s) 335, 1134, 1184, 2200, 2282
BseRI GAGGAG 4 cut(s) 241, 893, 896, 2615
BseSI GKGCMC 1 cut(s) 1213
BseXI GCAGC 2 cut(s) 451, 2727
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 2 cut(s) 565, 2368
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 7 cut(s) 5, 168, 470, 1179, 1574, 1645, 2526
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 3 cut(s) 14, 190, 2320
BsiHKCI CYCGRG 2 cut(s) 7, 1781
BsiSI CCGG 1 cut(s) 1382
BslFI GGGAC 2 cut(s) 1480, 3145
BslI CCNNNNNNNGG 1 cut(s) 1981
BsmAI GTCTC 7 cut(s) 842, 1778, 1871, 2162, 2301, 2337, 2740
BsmFI GGGAC 2 cut(s) 1480, 3145
BsmI GAATGC 2 cut(s) 1175, 2028
BsnI GGCC 7 cut(s) 5, 168, 470, 1179, 1574, 1645, 2526
BsoBI CYCGRG 2 cut(s) 7, 1781
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 4 cut(s) 14, 190, 1213, 2320
Bsp1407I TGTACA 3 cut(s) 1302, 2112, 2211
Bsp19I CCATGG 1 cut(s) 708
BspACI CCGC 2 cut(s) 1058, 2743
BspANI GGCC 7 cut(s) 5, 168, 470, 1179, 1574, 1645, 2526
BspCNI CTCAG 7 cut(s) 1259, 1412, 1694, 1749, 2347, 2853, 2998
BspHI TCATGA 4 cut(s) 636, 1035, 1627, 2344
BspLI GGNNCC 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 1282
BspPI GGATC 6 cut(s) 898, 1122, 1830, 2288, 2352, 2671
BspQI GCTCTTC 3 cut(s) 856, 2308, 3095
BspT104I TTCGAA 1 cut(s) 387
BspTI CTTAAG 1 cut(s) 2678
BsrDI GCAATG 2 cut(s) 2119, 2782
BsrGI TGTACA 3 cut(s) 1302, 2112, 2211
BsrI ACTGG 5 cut(s) 335, 1134, 1184, 2200, 2282
BssECI CCNNGG 3 cut(s) 708, 2485, 2900
BssT1I CCWWGG 1 cut(s) 708
Bst2UI CCWGG 1 cut(s) 1607
Bst6I CTCTTC 8 cut(s) 136, 212, 374, 856, 2160, 2308, 2972, 3095
BstAFI CTTAAG 1 cut(s) 2678
BstAUI TGTACA 3 cut(s) 1302, 2112, 2211
BstBI TTCGAA 1 cut(s) 387
BstC8I GCNNGC 4 cut(s) 1177, 1514, 2501, 3055
BstDSI CCRYGG 3 cut(s) 708, 2485, 2900
BstEII GGTNACC 1 cut(s) 1601
BstHHI GCGC 2 cut(s) 1175, 1660
BstMAI GTCTC 7 cut(s) 842, 1778, 1871, 2162, 2301, 2337, 2740
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 3 cut(s) 608, 1196, 2749
BstNI CCWGG 1 cut(s) 1607
BstNSI RCATGY 2 cut(s) 1459, 2119
BstPI GGTNACC 1 cut(s) 1601
BstSCI CCNGG 2 cut(s) 1380, 1605
BstSFI CTRYAG 2 cut(s) 868, 2435
BstSLI GKGCMC 1 cut(s) 1213
BstV1I GCAGC 2 cut(s) 451, 2727
BstV2I GAAGAC 1 cut(s) 3012
BstX2I RGATCY 7 cut(s) 172, 903, 1127, 1822, 2280, 2357, 2663
BstYI RGATCY 7 cut(s) 172, 903, 1127, 1822, 2280, 2357, 2663
BsuI GTATCC 2 cut(s) 787, 2801
BsuRI GGCC 7 cut(s) 5, 168, 470, 1179, 1574, 1645, 2526
BtgI CCRYGG 3 cut(s) 708, 2485, 2900
BtgZI GCGATG 1 cut(s) 2250
BtrI CACGTC 1 cut(s) 721
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 6 cut(s) 449, 546, 663, 2041, 2993, 3072
BveI ACCTGC 1 cut(s) 1282
Cac8I GCNNGC 4 cut(s) 1177, 1514, 2501, 3055
CaiI CAGNNNCTG 3 cut(s) 26, 1414, 2920
CciI TCATGA 4 cut(s) 636, 1035, 1627, 2344
CfoI GCGC 2 cut(s) 1175, 1660
Cfr13I GGNCC 2 cut(s) 2525, 2891
CseI GACGC 2 cut(s) 993, 2013
CsiI ACCWGGT 1 cut(s) 1605
Csp6I GTAC 6 cut(s) 365, 554, 1303, 2113, 2212, 2720
CspCI CAANNNNNGTGG 2 cut(s) 460, 495
CviQI GTAC 6 cut(s) 365, 554, 1303, 2113, 2212, 2720
DraI TTTAAA 3 cut(s) 459, 598, 1770
EaeI YGGCCR 4 cut(s) 166, 468, 1572, 1643
Eam1104I CTCTTC 8 cut(s) 136, 212, 374, 856, 2160, 2308, 2972, 3095
EarI CTCTTC 8 cut(s) 136, 212, 374, 856, 2160, 2308, 2972, 3095
Ecl136II GAGCTC 2 cut(s) 12, 188
Eco130I CCWWGG 1 cut(s) 708
Eco24I GRGCYC 2 cut(s) 14, 190
Eco32I GATATC 3 cut(s) 1066, 2085, 2293
Eco47I GGWCC 1 cut(s) 2891
Eco53kI GAGCTC 2 cut(s) 12, 188
Eco57I CTGAAG 3 cut(s) 1305, 1436, 1614
Eco88I CYCGRG 2 cut(s) 7, 1781
Eco91I GGTNACC 1 cut(s) 1601
EcoICRI GAGCTC 2 cut(s) 12, 188
EcoO65I GGTNACC 1 cut(s) 1601
EcoRI GAATTC 2 cut(s) 327, 957
EcoRII CCWGG 1 cut(s) 1605
EcoRV GATATC 3 cut(s) 1066, 2085, 2293
EcoT14I CCWWGG 1 cut(s) 708
EcoT22I ATGCAT 5 cut(s) 1313, 1554, 2097, 2121, 3177
EcoT38I GRGCYC 2 cut(s) 14, 190
ErhI CCWWGG 1 cut(s) 708
FaqI GGGAC 2 cut(s) 1480, 3145
FauNDI CATATG 3 cut(s) 2231, 2882, 2974
FbaI TGATCA 2 cut(s) 1405, 2947
FblI GTMKAC 2 cut(s) 678, 745
Fnu4HI GCNGC 2 cut(s) 440, 2741
FriOI GRGCYC 2 cut(s) 14, 190
Fsp4HI GCNGC 2 cut(s) 440, 2741
FspAI RTGCGCAY 1 cut(s) 1659
FspBI CTAG 8 cut(s) 51, 1164, 1509, 1623, 1811, 1836, 2102, 3135
FspI TGCGCA 1 cut(s) 1659
GlaI GCGC 2 cut(s) 1174, 1659
GluI GCNGC 2 cut(s) 440, 2741
GsaI CCCAGC 1 cut(s) 966
HaeIII GGCC 7 cut(s) 5, 168, 470, 1179, 1574, 1645, 2526
HapII CCGG 1 cut(s) 1382
HgaI GACGC 2 cut(s) 993, 2013
HhaI GCGC 2 cut(s) 1175, 1660
Hin6I GCGC 2 cut(s) 1173, 1658
HinP1I GCGC 2 cut(s) 1173, 1658
HincII GTYRAC 1 cut(s) 2193
HindII GTYRAC 1 cut(s) 2193
HindIII AAGCTT 4 cut(s) 393, 1503, 1884, 1946
HpaI GTTAAC 1 cut(s) 2193
HpaII CCGG 1 cut(s) 1382
HphI GGTGA 5 cut(s) 737, 997, 2425, 2660, 2779
Hpy166II GTNNAC 6 cut(s) 523, 679, 728, 746, 1303, 2193
Hpy8I GTNNAC 6 cut(s) 523, 679, 728, 746, 1303, 2193
Hpy99I CGWCG 2 cut(s) 725, 2189
HpyAV CCTTC 9 cut(s) 48, 1010, 1508, 1525, 1889, 2153, 2447, 2807, 3174
HpyCH4IV ACGT 4 cut(s) 110, 180, 720, 1255
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 1196, 2749
HpySE526I ACGT 4 cut(s) 110, 180, 720, 1255
HspAI GCGC 2 cut(s) 1173, 1658
Ksp22I TGATCA 2 cut(s) 1405, 2947
KspAI GTTAAC 1 cut(s) 2193
LguI GCTCTTC 3 cut(s) 856, 2308, 3095
LmnI GCTCC 4 cut(s) 17, 52, 185, 2902
Lsp1109I GCAGC 2 cut(s) 451, 2727
MabI ACCWGGT 1 cut(s) 1605
MaeI CTAG 8 cut(s) 51, 1164, 1509, 1623, 1811, 1836, 2102, 3135
MaeII ACGT 4 cut(s) 110, 180, 720, 1255
MaeIII GTNAC 6 cut(s) 661, 1099, 1601, 2062, 2413, 2705
MfeI CAATTG 2 cut(s) 237, 2698
MflI RGATCY 7 cut(s) 172, 903, 1127, 1822, 2280, 2357, 2663
MhlI GDGCHC 4 cut(s) 14, 190, 1213, 2320
MlsI TGGCCA 4 cut(s) 168, 470, 1574, 1645
MluNI TGGCCA 4 cut(s) 168, 470, 1574, 1645
MlyI GAGTC 6 cut(s) 198, 1517, 1824, 2869, 2911, 3167
MmeI TCCRAC 5 cut(s) 35, 676, 1476, 2096, 3268
Mox20I TGGCCA 4 cut(s) 168, 470, 1574, 1645
Mph1103I ATGCAT 5 cut(s) 1313, 1554, 2097, 2121, 3177
MroXI GAANNNNTTC 1 cut(s) 383
MscI TGGCCA 4 cut(s) 168, 470, 1574, 1645
MslI CAYNNNNRTG 3 cut(s) 723, 2471, 2613
Msp20I TGGCCA 4 cut(s) 168, 470, 1574, 1645
MspCI CTTAAG 1 cut(s) 2678
MspI CCGG 1 cut(s) 1382
MspR9I CCNGG 2 cut(s) 1382, 1607
MunI CAATTG 2 cut(s) 237, 2698
Mva1269I GAATGC 2 cut(s) 1175, 2028
MvaI CCWGG 1 cut(s) 1607
MwoI GCNNNNNNNGC 3 cut(s) 608, 1196, 2749
NciI CCSGG 1 cut(s) 1382
NcoI CCATGG 1 cut(s) 708
NdeI CATATG 3 cut(s) 2231, 2882, 2974
NlaIV GGNNCC 1 cut(s) 898
NmuCI GTSAC 3 cut(s) 661, 1099, 2413
NsbI TGCGCA 1 cut(s) 1659
NsiI ATGCAT 5 cut(s) 1313, 1554, 2097, 2121, 3177
NspI RCATGY 2 cut(s) 1459, 2119
NspV TTCGAA 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 723
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 4 cut(s) 636, 1035, 1627, 2344
PciSI GCTCTTC 3 cut(s) 856, 2308, 3095
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PctI GAATGC 2 cut(s) 1175, 2028
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 7 cut(s) 224, 1346, 1448, 1954, 2181, 2431, 2831
PfoI TCCNGGA 1 cut(s) 1380
PkrI GCNGC 2 cut(s) 441, 2742
Ple19I CGATCG 1 cut(s) 127
PleI GAGTC 6 cut(s) 198, 1517, 1823, 2868, 2911, 3166
PpsI GAGTC 6 cut(s) 198, 1517, 1823, 2868, 2911, 3166
PshBI ATTAAT 1 cut(s) 1653
Psp124BI GAGCTC 2 cut(s) 14, 190
Psp6I CCWGG 1 cut(s) 1605
PspEI GGTNACC 1 cut(s) 1601
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 1 cut(s) 1605
PspN4I GGNNCC 1 cut(s) 898
PspPI GGNCC 2 cut(s) 2525, 2891
PspXI VCTCGAGB 1 cut(s) 7
PstNI CAGNNNCTG 3 cut(s) 26, 1414, 2920
PsuI RGATCY 7 cut(s) 172, 903, 1127, 1822, 2280, 2357, 2663
PvuI CGATCG 1 cut(s) 127
RsaI GTAC 6 cut(s) 366, 555, 1304, 2114, 2213, 2721
RsaNI GTAC 6 cut(s) 365, 554, 1303, 2113, 2212, 2720
RseI CAYNNNNRTG 3 cut(s) 723, 2471, 2613
SacI GAGCTC 2 cut(s) 14, 190
SapI GCTCTTC 3 cut(s) 856, 2308, 3095
SatI GCNGC 2 cut(s) 440, 2741
Sau96I GGNCC 2 cut(s) 2525, 2891
SchI GAGTC 6 cut(s) 198, 1517, 1824, 2869, 2911, 3167
ScrFI CCNGG 2 cut(s) 1382, 1607
SduI GDGCHC 4 cut(s) 14, 190, 1213, 2320
SexAI ACCWGGT 1 cut(s) 1605
SfcI CTRYAG 2 cut(s) 868, 2435
Sfr274I CTCGAG 1 cut(s) 7
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 1 cut(s) 2891
SlaI CTCGAG 1 cut(s) 7
SmiI ATTTAAAT 1 cut(s) 1770
SmiMI CAYNNNNRTG 3 cut(s) 723, 2471, 2613
SmlI CTYRAG 5 cut(s) 7, 1940, 1949, 1979, 2678
SmoI CTYRAG 5 cut(s) 7, 1940, 1949, 1979, 2678
SpeI ACTAGT 1 cut(s) 1835
SsiI CCGC 2 cut(s) 1058, 2743
SspI AATATT 3 cut(s) 2176, 2351, 3191
SspMI CTAG 8 cut(s) 51, 1164, 1509, 1623, 1811, 1836, 2102, 3135
SstI GAGCTC 2 cut(s) 14, 190
StyD4I CCNGG 2 cut(s) 1380, 1605
StyI CCWWGG 1 cut(s) 708
SwaI ATTTAAAT 1 cut(s) 1770
TaiI ACGT 4 cut(s) 113, 183, 723, 1258
TaqI TCGA 9 cut(s) 8, 114, 219, 269, 387, 1216, 2184, 2589, 3281
TatI WGTACW 4 cut(s) 1302, 2112, 2211, 2719
TfiI GAWTC 7 cut(s) 224, 1346, 1448, 1954, 2181, 2431, 2831
TscAI CASTG 6 cut(s) 449, 546, 670, 2041, 2993, 3072
TseFI GTSAC 3 cut(s) 661, 1099, 2413
TseI GCWGC 2 cut(s) 439, 2740
Tsp45I GTSAC 3 cut(s) 661, 1099, 2413
TspGWI ACGGA 5 cut(s) 153, 696, 2285, 2441, 2917
TspRI CASTG 6 cut(s) 449, 546, 670, 2041, 2993, 3072
Vha464I CTTAAG 1 cut(s) 2678
VpaK11BI GGWCC 1 cut(s) 2891
VspI ATTAAT 1 cut(s) 1653
XapI RAATTY 7 cut(s) 297, 327, 801, 957, 1740, 1765, 3040
XceI RCATGY 2 cut(s) 1459, 2119
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 678, 745
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 8 cut(s) 51, 1164, 1509, 1623, 1811, 1836, 2102, 3135
Zsp2I ATGCAT 5 cut(s) 1313, 1554, 2097, 2121, 3177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.