RchiOBHm_Chr5g0061291

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000085
Physical Location & Seq
Reverse (-)
66693939 .. 66701180
7242 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33767

Sequence Viewer

Length: 4695 bp
ATGGCCTTGGAATCCCCAACGGCCTCTGCATCACTTCCTACATCAGTTGAATCATCGGCTCCTGGGTGGAAGTATGAGGTGTTTTTGAGTTTCAGGAGTGAAGACACTCGGCGGGGTTTTACTTCTCATTTGTACAATGAATTGCAAAACAGAGAAATCAAGACATTCATGGATATCGACGGAACTAGTTTGGATAGAGGAGAGGGGATTTCTCAAAGTATCCTAGAGGCCATTCAAGAATCAAGATTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCCAGTTGGTGTTTGGATGAACTCACACAGATTTTGCAGTGCATGGAAAATAAGGAAACAATTCTGCCAGTTTTCTATCACGTGGATCCCTCTGAAGTGCGCTCTCAAAGGGGGGTGTGCGGAGCGGCCTTGGCTGCACATGAAGAGCGGTTTAAAGATCAAAAAGAGAGGCTGATGAAATGGAGGGACGCCTTGAACAAAATCAGCAGCATCTTTGGCTGGGTATGTGGCAGTTCTGAGCCAGAAGGACAGCTTGTCCAACAAATCGCGGAATTTGTCTGCAGCAAACCTATTGAAATTGAGTCGACGGAAGATATTGAAACTCATGGAACTTCTCCTCGTTGGAAGTATGATGTGTTTATAAATTTCAGGGGTGAGGACACACTACGTAAGGGTTTTCTGTCCGATTTACTAGCTGAATTGCAAAAGAAACCAGCACACCTGAACACAGTAAAGAATGATGAACCACTTCCAAAAGGGCGGAATATATCTTCGTATCTCCTAAACGCCATAGAAGAGGTGAGGTTTGCCATTGTTGTTCTTTCGCAAGACTTTGCTTCTTCATCATGGTGCCTAGAGGAACTAGCAAAGATTTTTGAATGCATGGAAAACCAAAATAGAATTCTGCCAATCTTTTATCATGTGGATCCCTCTGAGGTGCGACATCAAAGGGGGAGTTTTGGAGATGCCTTCGCTGTACATGAAGAAAGATTTCGAGATAATCATGAGAAGGTCAGGCGGTGGAGACGTGTTTTATCAATGGTGGCCAATCTCCCTGGATGGTGTTCGGATAAGTACAAGAATGAAGGAGAACTTATCAAAGACGTTGCGAGTGCTGTGTGCAGTAGACTAGTTCAGTCTACAGAGTCGACAGAAGATTTTGAAGCAACAGGAGATGTCATGGACCGAACTTCTCCCCAGCCTGACCCAGCTCCTGTGTGGAAGAATGATGTGCTTTCGGGTTTCAGGATGGAACACACTCGCAGGGGTTTTGTAGCCCATGCAGACCATGAATTGCCAAACACAGGACTAAGTAAAACATTCAAGGATGAAGACCAGCTTGAAAAAGGGGTAGCCCTCCCCTCTGCATCTCTTTCCTCAACTGATCCAAGTCCTCAACCAGCTCTTGGGCAGAAGAATGATGAGTTTTTGGGTTTCAGGGGAAACGACAATCATAGGGGTTATGTCACCCATTCAAGTCATGAAATACAAAACACCAGGGTGACTAAGACATTCACCGATGAAGAACATCTTGAAAAAGGGATAGCTGCGTCTCCGCCTTCATCAGCTGAACGAGATCCTCGGCCGAAGTATGATGTGTTTTTGAGTTACAGGGGGGAAGACACTCGTAGGGGTTTTATAGCCTATTTATACCGTGAATTGCAAAACACCAGAGTGATCAAAACTTTCAAGGATGACGTGCAACTAGAAAAAGGGACGGTTATTGCTTCAAATCTCCTAACGGCAATCGAAGAATCAAGGTTTGCGATCATTGTGTTGTCAAAAAATTATGCTTCTTCTTCATGGTGTTTGGATGAACTTGTAAAGATTTTCCAGTGCATGAAAGATAAGAACAGAATTTTCCCAATTTTTTATGAGGTGGAACCCACTGATGTACGATTTCAAAAGGAAGAGACAGGTTTTGGAGAAGCTTTCATTAAGCATGAAGCTAGACAAGACATTGAGAAGATGAACCAGTGGAGAGCTGCTTTAAAAGGAGTGTCTGGAATCTCTGGGTGGCATTCAACCAACTATCCGACGGATATAGAACTAATTGAAGACATTGTGAAACATTTGTGGGAGAAAGTACTTGAAGCACCCACCGGAAATTTTGAAACATTTGAAGCGACAAGGCAAGCCATGGATGAGGTTATGAAGGTAATAAAAGATGATCAAGTCACTGTCCTTGGGGTGCATGGAGTGGCTGGCGTTGGAAAGACAACGATGGTCAAACATGTTAGTGCAGAAGCCCAGAAAGGTGGGCTTTTTGATCATGTGATTATGGTTGTTGTATCCAAAGCCCCCGACTTGCAAGAAATTCAAGGCACATTGGCAGATTCGCTGGGCTTGGAGGAGGGGATCATGAGAGGAAACAGCATGCTTATAATCTTGGACGACATTTGGGAGAGTTTTGAACTCTCGAGCATTGGAATTCCCAATCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTCACCACAAGGAAAAGCAATATATGCCATTCTATGGGGTGCCGTGCCCAGATCCCTCTCAATGTTCTATCGGAGGGAGATTCGTGGAAGTTATTCATGAAGACGTCGGGGAAGTCTTTTCATGAATCTACCGTTTCCTATGATGAAGCGAGAAAGATAGCTGGAGAATGCGGTGGTCTCCCAAAAGCAATAAAAGCAGCTGCAAGGTCACTTGGAGATAAATACATGGACGAATGGAATGAAGCAGTTGAAACAGCATTTGGAGATGACTGCTTGGACGAATGGAATGAAACAGCTCATCCTGTCAACTTCGAAGCTGAGGAAGACGTATTTCATTCATTGGCAACTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCAGAAAATTATGATATCCCATTTGAAGTGTTGCTCAGGTATGGGATTGGAATAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGATAGAGCACATTCTGTGGTCAAGTACCTCAAAGCTTCTAGATTGCTTTTGGATGGTACAAAGAAGGGATGCACAAGGATGACTGATGACATTCGAAATCTGGCCATGTCCATTATGCGGTCCGAAGAAGGTGATGGGTTCTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCAGGATTTTGGCAAGGAATTACTTTCCGGCTCCGCAAAATCTGGGATTGGCCAATGGATGCTCATGAAGGCTACTCAATAATCTCACTCATGAAAAACAAGCTTCATAAGCTTCCCGAATATTTGGTATGTTCAGAGCTCCAAATTTTATTGTTACAAAGTAATACTGGTATACGTGACATCCCAGAAACCTTTCTTCAAAGTCCTAATGCGCTAAGGGTCTTTGATCTTAGCTTCACTAGCATTTCATCACTACCTTCATCATTCAGTTTCCTAACCAACCTTCAAGTTTTGAATTTGGATTTTTGCCATAATTTAATGATATCGAGGTTGTATAGATTGGAAGAACTGCACATGAAATTTCAGTTTGGGAAGTGGGGAAGTAAAATTGAGGATGCAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGGAGTTTACATATCTGATGCTGCATGCTTGCCTAAAAGTGTTGAGTACAAGCCGAATTGGGTAGAATTTGATATATGCATCAGCAGAGATGCCCCTTGCGTGTCGCTCCCTCAACATGATGGTTTTTCAAGAACCTTGACTCTTGACACAACAATCAATTGCTTACCGGATTGGTTTATCAGCGTTGTGACAGAGAAAGTGGAGAAGCTAGAGTACACAGATTGTGAAGACTTGAATGACATCGTTGGGCAATATGACCATGGCAGGTTACTTGCACTGAAATATCTCTCTGTGAACGGGCACCATAAGAACTTGACAGAGATAATCAACACAATGACACGGGTTTCAAATAAACCAGTATTTGAGAACTTAGAACAGTTGCATCTGTACCGCTTATATTCCCTCAAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTACACAAGCTAAAGTTGTTGAAAGTGCAAACTTGTTGTAGCTTGGTGAATGCACTTTTACCATCGACATTGTTGCAGAGACTACAAAATCTGGAAGAAATCATTTGTGTAAATCTAGAAGGAATGAAATATGTATTTGGATCTGATGAAGGGCTTGATCCAGAAAATACTTTCTTGAGGAAATTGAGGGAAATGAGATTGTCTACTCTAGACAAACTAGTAAGCATATGTAATGGTCCTGCTCCGCATGCAATCTTCCATAATCTTAAAGTTTTGGCAATTTGCAAATGCAAGGAATTGAAAAATCTCTTCACAATAGGTGTAGCTGAATGTCTTTCTCAGTTGGAAGACCTTTCCGTACAGCAGTGTCATAGCTTGGACAGAGTCATTGAAGTAAGCAATAGCTTGACCAGCAAGATTAAACTTCCGGCGTTGAAGAAATTAGCTTTGATAGATCTTCCGGTGCTCACAAGGTTCTGCACTGAAAGTGTTACTATTGAAACTGAGTGCCCTTCATTGGAATACTTGTACGTGGAGAAGTGCCTCCAGTTTTCATATTTTGCTTACGATTTCGACAACAAGAACCAAGTCCACTTCAATGATGAACAACATTTGAGTTCTTTAGTGAAAAGGTGGGAGGAAGTGCATACTCGGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1564

Amino Acids

177.52

Weight (kDa)

5.24

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 192 2.1e-48 TIR domain
TIR_2 PF13676 27 - 124 3.1e-16 TIR domain
TIR PF01582 210 - 389 1.2e-45 TIR domain
TIR_2 PF13676 213 - 311 8.8e-09 TIR domain
TIR PF01582 531 - 702 6e-47 TIR domain
TIR_2 PF13676 534 - 630 1.5e-13 TIR domain
NB-ARC PF00931 713 - 862 4.3e-24 NB-ARC domain
LRR_8 PF13855 1091 - 1149 1.9e-07 Leucine rich repeat
LRR_RPS2 PF23247 1322 - 1468 2e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1454 - 1529 2.4e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 644, 2387
AatII GACGTC 1 cut(s) 2585
Acc36I ACCTGC 1 cut(s) 3849
AccB1I GGYRCC 3 cut(s) 852, 2517, 3894
AccB7I CCANNNNNTGG 2 cut(s) 1409, 2512
AccBSI CCGCTC 2 cut(s) 407, 430
AccI GTMKAC 6 cut(s) 587, 1129, 1142, 1151, 3307, 4238
AccII CGCG 1 cut(s) 551
AcoI YGGCCR 5 cut(s) 1047, 1586, 3057, 3128, 3185
AcuI CTGAAG 1 cut(s) 396
AcvI CACGTG 1 cut(s) 364
AcyI GRCGYC 2 cut(s) 471, 2582
AfiI CCNNNNNNNGG 6 cut(s) 1307, 1409, 2512, 3072, 3137, 4552
AflIII ACRYGT 2 cut(s) 1030, 2236
AhdI GACNNNNNGTC 1 cut(s) 536
AhlI ACTAGT 3 cut(s) 185, 1132, 4252
AjiI CACGTC 2 cut(s) 1031, 1702
AjnI CCWGG 4 cut(s) 61, 1057, 1499, 4027
AjuI GAANNNNNNNTTGG 6 cut(s) 888, 920, 2721, 2753, 2828, 2860
AleI CACNNNNGTG 2 cut(s) 1502, 1676
Alw21I GWGCWC 3 cut(s) 2965, 3276, 4503
Alw26I GTCTC 6 cut(s) 1021, 1560, 1910, 2660, 4038, 4108
AlwNI CAGNNNCTG 1 cut(s) 1217
Ama87I CYCGRG 1 cut(s) 2422
AoxI GGCC 9 cut(s) 3, 21, 228, 408, 1047, 1586, 3057, 3128, 3185
ApeKI GCWGC 9 cut(s) 416, 489, 564, 1550, 1988, 2675, 2678, 2856, 3614
Asp700I GAANNNNTTC 5 cut(s) 342, 750, 993, 2570, 3327
AspLEI GCGC 2 cut(s) 384, 3349
AspS9I GGNCC 3 cut(s) 1186, 3075, 4271
AsuHPI GGTGA 9 cut(s) 668, 814, 1462, 1510, 1516, 2473, 3098, 4028, 4093
AsuII TTCGAA 2 cut(s) 2790, 3049
AvaI CYCGRG 1 cut(s) 2422
AvaII GGWCC 3 cut(s) 1186, 3075, 4271
BaeGI GKGCMC 3 cut(s) 2527, 3897, 4547
BalI TGGCCA 4 cut(s) 1049, 3059, 3130, 3187
BamHI GGATCC 2 cut(s) 367, 928
BanI GGYRCC 3 cut(s) 852, 2517, 3894
BanII GRGCYC 1 cut(s) 3276
BarI GAAGNNNNNNTAC 2 cut(s) 3791, 3823
BbrPI CACGTG 1 cut(s) 364
BbsI GAAGAC 8 cut(s) 108, 1341, 1629, 2067, 2585, 2808, 3828, 4389
Bbv12I GWGCWC 3 cut(s) 2965, 3276, 4503
BbvCI CCTCAGC 1 cut(s) 2796
BbvI GCAGC 9 cut(s) 403, 501, 576, 1537, 1975, 2665, 2687, 2843, 3601
BccI CCATC 7 cut(s) 1056, 1246, 2221, 3002, 3083, 3707, 4105
BceAI ACGGC 3 cut(s) 36, 1761, 2505
BcgI CGANNNNNNTGC 2 cut(s) 4090, 4124
BciT130I CCWGG 4 cut(s) 63, 1059, 1501, 4029
BciVI GTATCC 2 cut(s) 230, 2305
BclI TGATCA 3 cut(s) 1680, 2173, 2272
BcoDI GTCTC 6 cut(s) 1021, 1560, 1910, 2660, 4038, 4108
BcuI ACTAGT 3 cut(s) 185, 1132, 4252
BfmI CTRYAG 2 cut(s) 562, 1143
BfuAI ACCTGC 1 cut(s) 3849
BfuI GTATCC 2 cut(s) 230, 2305
BglII AGATCT 1 cut(s) 4489
BmcAI AGTACT 1 cut(s) 2091
Bme1390I CCNGG 4 cut(s) 63, 1059, 1501, 4029
Bme18I GGWCC 3 cut(s) 1186, 3075, 4271
BmeRI GACNNNNNGTC 1 cut(s) 536
BmeT110I CYCGRG 1 cut(s) 2422
BmgBI CACGTC 2 cut(s) 1031, 1702
BmgT120I GGNCC 3 cut(s) 1186, 3075, 4271
BmiI GGNNCC 8 cut(s) 60, 369, 854, 930, 1887, 2519, 3167, 3896
BmrFI CCNGG 4 cut(s) 63, 1059, 1501, 4029
BpiI GAAGAC 8 cut(s) 108, 1341, 1629, 2067, 2585, 2808, 3828, 4389
BpmI CTGGAG 2 cut(s) 2661, 4565
Bpu10I CCTNAGC 3 cut(s) 2796, 2900, 3350
Bpu14I TTCGAA 2 cut(s) 2790, 3049
BpuEI CTTGAG 2 cut(s) 3983, 4231
BsaAI YACGTR 4 cut(s) 364, 671, 3311, 4567
BsaHI GRCGYC 2 cut(s) 471, 2582
BsaI GGTCTC 2 cut(s) 2660, 4038
BsaWI WCCGGW 3 cut(s) 2105, 3760, 4495
Bsc4I CCNNNNNNNGG 6 cut(s) 1307, 1409, 2512, 3072, 3137, 4552
Bse1I ACTGG 8 cut(s) 285, 350, 1837, 1978, 3307, 3574, 3950, 4582
BseBI CCWGG 4 cut(s) 63, 1059, 1501, 4029
BseLI CCNNNNNNNGG 6 cut(s) 1307, 1409, 2512, 3072, 3137, 4552
BseMII CTCAG 6 cut(s) 510, 927, 2787, 2914, 4388, 4530
BseNI ACTGG 8 cut(s) 285, 350, 1837, 1978, 3307, 3574, 3950, 4582
BseRI GAGGAG 4 cut(s) 213, 609, 2369, 3137
BseSI GKGCMC 3 cut(s) 2527, 3897, 4547
BseX3I CGGCCG 1 cut(s) 1586
BseXI GCAGC 9 cut(s) 403, 501, 576, 1537, 1975, 2665, 2687, 2843, 3601
BseYI CCCAGC 4 cut(s) 501, 1200, 1210, 2344
BsgI GTGCAG 5 cut(s) 402, 1144, 2265, 3470, 4498
Bsh1236I CGCG 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 1589
BshFI GGCC 9 cut(s) 5, 23, 230, 410, 1049, 1588, 3059, 3130, 3187
BshNI GGYRCC 3 cut(s) 852, 2517, 3894
BsiEI CGRYCG 1 cut(s) 1589
BsiHKAI GWGCWC 3 cut(s) 2965, 3276, 4503
BsiHKCI CYCGRG 1 cut(s) 2422
BsiSI CCGG 5 cut(s) 2106, 3163, 3761, 4463, 4496
BslFI GGGAC 2 cut(s) 482, 1732
BslI CCNNNNNNNGG 6 cut(s) 1307, 1409, 2512, 3072, 3137, 4552
BsmAI GTCTC 6 cut(s) 1021, 1560, 1910, 2660, 4038, 4108
BsmBI CGTCTC 2 cut(s) 1021, 1560
BsmFI GGGAC 2 cut(s) 482, 1732
BsmI GAATGC 4 cut(s) 887, 2023, 2651, 4090
BsnI GGCC 9 cut(s) 5, 23, 230, 410, 1049, 1588, 3059, 3130, 3187
Bso31I GGTCTC 2 cut(s) 2660, 4038
BsoBI CYCGRG 1 cut(s) 2422
Bsp119I TTCGAA 2 cut(s) 2790, 3049
Bsp1286I GDGCHC 6 cut(s) 2527, 2965, 3276, 3897, 4503, 4547
Bsp1407I TGTACA 2 cut(s) 132, 979
Bsp19I CCATGG 2 cut(s) 2142, 3853
BspANI GGCC 9 cut(s) 5, 23, 230, 410, 1049, 1588, 3059, 3130, 3187
BspCNI CTCAG 6 cut(s) 511, 928, 2788, 2913, 4387, 4531
BspFNI CGCG 1 cut(s) 551
BspHI TCATGA 7 cut(s) 1006, 1483, 2364, 2574, 2599, 3199, 3225
BspLI GGNNCC 8 cut(s) 60, 369, 854, 930, 1887, 2519, 3167, 3896
BspMAI CTGCAG 1 cut(s) 566
BspMI ACCTGC 1 cut(s) 3849
BspQI GCTCTTC 1 cut(s) 420
BspT104I TTCGAA 2 cut(s) 2790, 3049
BspT107I GGYRCC 3 cut(s) 852, 2517, 3894
BspTNI GGTCTC 2 cut(s) 2660, 4038
BsrBI CCGCTC 2 cut(s) 407, 430
BsrGI TGTACA 2 cut(s) 132, 979
BsrI ACTGG 8 cut(s) 285, 350, 1837, 1978, 3307, 3574, 3950, 4582
BssNAI GTATAC 1 cut(s) 3308
BssNI GRCGYC 2 cut(s) 471, 2582
BssT1I CCWWGG 5 cut(s) 6, 411, 2142, 2188, 3853
Bst1107I GTATAC 1 cut(s) 3308
Bst2UI CCWGG 4 cut(s) 63, 1059, 1501, 4029
Bst4CI ACNGT 6 cut(s) 733, 1658, 1723, 2185, 2611, 3972
Bst6I CTCTTC 4 cut(s) 420, 792, 1908, 4349
BstACI GRCGYC 2 cut(s) 471, 2582
BstAPI GCANNNNNTGC 1 cut(s) 2502
BstAUI TGTACA 2 cut(s) 132, 979
BstBAI YACGTR 4 cut(s) 364, 671, 3311, 4567
BstBI TTCGAA 2 cut(s) 2790, 3049
BstC8I GCNNGC 6 cut(s) 2139, 2209, 2381, 3619, 3623, 4284
BstDSI CCRYGG 2 cut(s) 2142, 3853
BstFNI CGCG 1 cut(s) 551
BstHHI GCGC 2 cut(s) 384, 3349
BstMAI GTCTC 6 cut(s) 1021, 1560, 1910, 2660, 4038, 4108
BstMCI CGRYCG 1 cut(s) 1589
BstNI CCWGG 4 cut(s) 63, 1059, 1501, 4029
BstNSI RCATGY 4 cut(s) 2240, 2383, 3621, 4286
BstSCI CCNGG 4 cut(s) 61, 1057, 1499, 4027
BstSFI CTRYAG 2 cut(s) 562, 1143
BstSLI GKGCMC 3 cut(s) 2527, 3897, 4547
BstSNI TACGTA 1 cut(s) 671
BstUI CGCG 1 cut(s) 551
BstV1I GCAGC 9 cut(s) 403, 501, 576, 1537, 1975, 2665, 2687, 2843, 3601
BstV2I GAAGAC 8 cut(s) 108, 1341, 1629, 2067, 2585, 2808, 3828, 4389
BstX2I RGATCY 6 cut(s) 367, 928, 1579, 2529, 4175, 4489
BstXI CCANNNNNNTGG 1 cut(s) 2261
BstYI RGATCY 6 cut(s) 367, 928, 1579, 2529, 4175, 4489
BstZ17I GTATAC 1 cut(s) 3308
BstZI CGGCCG 1 cut(s) 1586
BsuI GTATCC 2 cut(s) 230, 2305
BsuRI GGCC 9 cut(s) 5, 23, 230, 410, 1049, 1588, 3059, 3130, 3187
BtgI CCRYGG 2 cut(s) 2142, 3853
BtrI CACGTC 2 cut(s) 1031, 1702
BtsI GCAGTG 2 cut(s) 326, 4406
BtsIMutI CAGTG 8 cut(s) 326, 1844, 1890, 1985, 2181, 3869, 4406, 4515
BveI ACCTGC 1 cut(s) 3849
Cac8I GCNNGC 6 cut(s) 2139, 2209, 2381, 3619, 3623, 4284
CaiI CAGNNNCTG 1 cut(s) 1217
CciI TCATGA 7 cut(s) 1006, 1483, 2364, 2574, 2599, 3199, 3225
CfoI GCGC 2 cut(s) 384, 3349
Cfr13I GGNCC 3 cut(s) 1186, 3075, 4271
CpoI CGGWCCG 1 cut(s) 3075
CseI GACGC 2 cut(s) 479, 1542
CspI CGGWCCG 1 cut(s) 3075
DraI TTTAAA 3 cut(s) 436, 1995, 3585
DriI GACNNNNNGTC 1 cut(s) 536
EaeI YGGCCR 5 cut(s) 1047, 1586, 3057, 3128, 3185
EagI CGGCCG 1 cut(s) 1586
Eam1104I CTCTTC 4 cut(s) 420, 792, 1908, 4349
Eam1105I GACNNNNNGTC 1 cut(s) 536
EarI CTCTTC 4 cut(s) 420, 792, 1908, 4349
EciI GGCGGA 2 cut(s) 778, 1548
Ecl136II GAGCTC 1 cut(s) 3274
EclXI CGGCCG 1 cut(s) 1586
Eco105I TACGTA 1 cut(s) 671
Eco130I CCWWGG 5 cut(s) 6, 411, 2142, 2188, 3853
Eco24I GRGCYC 1 cut(s) 3276
Eco31I GGTCTC 2 cut(s) 2660, 4038
Eco32I GATATC 3 cut(s) 175, 2881, 3459
Eco47I GGWCC 3 cut(s) 1186, 3075, 4271
Eco52I CGGCCG 1 cut(s) 1586
Eco53kI GAGCTC 1 cut(s) 3274
Eco57I CTGAAG 1 cut(s) 396
Eco72I CACGTG 1 cut(s) 364
Eco88I CYCGRG 1 cut(s) 2422
EcoICRI GAGCTC 1 cut(s) 3274
EcoRI GAATTC 2 cut(s) 903, 2433
EcoRII CCWGG 4 cut(s) 61, 1057, 1499, 4027
EcoRV GATATC 3 cut(s) 175, 2881, 3459
EcoT14I CCWWGG 5 cut(s) 6, 411, 2142, 2188, 3853
EcoT22I ATGCAT 2 cut(s) 887, 3674
EcoT38I GRGCYC 1 cut(s) 3276
ErhI CCWWGG 5 cut(s) 6, 411, 2142, 2188, 3853
Esp3I CGTCTC 2 cut(s) 1021, 1560
FaqI GGGAC 2 cut(s) 482, 1732
FauI CCCGC 1 cut(s) 105
FauNDI CATATG 1 cut(s) 4262
FbaI TGATCA 3 cut(s) 1680, 2173, 2272
FblI GTMKAC 6 cut(s) 587, 1129, 1142, 1151, 3307, 4238
FriOI GRGCYC 1 cut(s) 3276
GlaI GCGC 2 cut(s) 383, 3348
GsaI CCCAGC 4 cut(s) 505, 1204, 1214, 2348
GsuI CTGGAG 2 cut(s) 2661, 4565
HaeIII GGCC 9 cut(s) 5, 23, 230, 410, 1049, 1588, 3059, 3130, 3187
HapII CCGG 5 cut(s) 2106, 3163, 3761, 4463, 4496
HgaI GACGC 2 cut(s) 479, 1542
HhaI GCGC 2 cut(s) 384, 3349
Hin1I GRCGYC 2 cut(s) 471, 2582
Hin6I GCGC 2 cut(s) 382, 3347
HinP1I GCGC 2 cut(s) 382, 3347
HincII GTYRAC 4 cut(s) 588, 1152, 2785, 3567
HindII GTYRAC 4 cut(s) 588, 1152, 2785, 3567
HindIII AAGCTT 4 cut(s) 1932, 2988, 3236, 3245
HpaII CCGG 5 cut(s) 2106, 3163, 3761, 4463, 4496
HphI GGTGA 9 cut(s) 668, 814, 1462, 1510, 1516, 2473, 3098, 4028, 4093
Hpy99I CGWCG 4 cut(s) 182, 592, 2044, 2587
HpyCH4III ACNGT 6 cut(s) 733, 1658, 1723, 2185, 2611, 3972
HpyCH4IV ACGT 9 cut(s) 363, 670, 1030, 1107, 1701, 2582, 2805, 3310, 4566
HpySE526I ACGT 9 cut(s) 363, 670, 1030, 1107, 1701, 2582, 2805, 3310, 4566
Hsp92I GRCGYC 2 cut(s) 471, 2582
HspAI GCGC 2 cut(s) 382, 3347
Ksp22I TGATCA 3 cut(s) 1680, 2173, 2272
LguI GCTCTTC 1 cut(s) 420
LmnI GCTCC 7 cut(s) 64, 404, 1219, 3171, 3279, 3705, 4282
Lsp1109I GCAGC 9 cut(s) 403, 501, 576, 1537, 1975, 2665, 2687, 2843, 3601
MaeII ACGT 9 cut(s) 363, 670, 1030, 1107, 1701, 2582, 2805, 3310, 4566
MbiI CCGCTC 2 cut(s) 407, 430
MfeI CAATTG 1 cut(s) 3751
MflI RGATCY 6 cut(s) 367, 928, 1579, 2529, 4175, 4489
MhlI GDGCHC 6 cut(s) 2527, 2965, 3276, 3897, 4503, 4547
MlsI TGGCCA 4 cut(s) 1049, 3059, 3130, 3187
MluNI TGGCCA 4 cut(s) 1049, 3059, 3130, 3187
MlyI GAGTC 4 cut(s) 593, 1157, 3727, 4428
MmeI TCCRAC 6 cut(s) 565, 605, 2063, 2194, 3558, 4359
Mox20I TGGCCA 4 cut(s) 1049, 3059, 3130, 3187
Mph1103I ATGCAT 2 cut(s) 887, 3674
MroXI GAANNNNTTC 5 cut(s) 342, 750, 993, 2570, 3327
MscI TGGCCA 4 cut(s) 1049, 3059, 3130, 3187
MseI TTAA 7 cut(s) 435, 1941, 1994, 3452, 3584, 4302, 4455
MslI CAYNNNNRTG 6 cut(s) 850, 1502, 1676, 2241, 3032, 4632
Msp20I TGGCCA 4 cut(s) 1049, 3059, 3130, 3187
MspA1I CMGCKG 2 cut(s) 1571, 2678
MspI CCGG 5 cut(s) 2106, 3163, 3761, 4463, 4496
MspR9I CCNGG 4 cut(s) 63, 1059, 1501, 4029
MunI CAATTG 1 cut(s) 3751
Mva1269I GAATGC 4 cut(s) 887, 2023, 2651, 4090
MvaI CCWGG 4 cut(s) 63, 1059, 1501, 4029
MvnI CGCG 1 cut(s) 551
NcoI CCATGG 2 cut(s) 2142, 3853
NdeI CATATG 1 cut(s) 4262
NlaIV GGNNCC 8 cut(s) 60, 369, 854, 930, 1887, 2519, 3167, 3896
NmeAIII GCCGAG 3 cut(s) 88, 1564, 4666
NmuCI GTSAC 6 cut(s) 1468, 1504, 2179, 2685, 3311, 3781
NsiI ATGCAT 2 cut(s) 887, 3674
NspI RCATGY 4 cut(s) 2240, 2383, 3621, 4286
NspV TTCGAA 2 cut(s) 2790, 3049
OliI CACNNNNGTG 2 cut(s) 1502, 1676
PaeI GCATGC 3 cut(s) 2383, 3621, 4286
PaeR7I CTCGAG 1 cut(s) 2422
PagI TCATGA 7 cut(s) 1006, 1483, 2364, 2574, 2599, 3199, 3225
PciI ACATGT 1 cut(s) 2236
PciSI GCTCTTC 1 cut(s) 420
PctI GAATGC 4 cut(s) 887, 2023, 2651, 4090
PdmI GAANNNNTTC 5 cut(s) 342, 750, 993, 2570, 3327
PfeI GAWTC 8 cut(s) 11, 50, 239, 1757, 2010, 2339, 2558, 2603
PflFI GACNNNGTC 1 cut(s) 4418
PflMI CCANNNNNTGG 2 cut(s) 1409, 2512
PleI GAGTC 4 cut(s) 592, 1156, 3727, 4427
PmaCI CACGTG 1 cut(s) 364
PmlI CACGTG 1 cut(s) 364
PpsI GAGTC 4 cut(s) 592, 1156, 3727, 4427
Ppu21I YACGTR 4 cut(s) 364, 671, 3311, 4567
PscI ACATGT 1 cut(s) 2236
PsiI TTATAA 2 cut(s) 644, 2387
Psp124BI GAGCTC 1 cut(s) 3276
Psp6I CCWGG 4 cut(s) 61, 1057, 1499, 4027
PspCI CACGTG 1 cut(s) 364
PspFI CCCAGC 4 cut(s) 501, 1200, 1210, 2344
PspGI CCWGG 4 cut(s) 61, 1057, 1499, 4027
PspN4I GGNNCC 8 cut(s) 60, 369, 854, 930, 1887, 2519, 3167, 3896
PspPI GGNCC 3 cut(s) 1186, 3075, 4271
PstI CTGCAG 1 cut(s) 566
PstNI CAGNNNCTG 1 cut(s) 1217
PsuI RGATCY 6 cut(s) 367, 928, 1579, 2529, 4175, 4489
PsyI GACNNNGTC 1 cut(s) 4418
PvuII CAGCTG 2 cut(s) 1571, 2678
RseI CAYNNNNRTG 6 cut(s) 850, 1502, 1676, 2241, 3032, 4632
Rsr2I CGGWCCG 1 cut(s) 3075
RsrII CGGWCCG 1 cut(s) 3075
SacI GAGCTC 1 cut(s) 3276
SalI GTCGAC 2 cut(s) 586, 1150
SapI GCTCTTC 1 cut(s) 420
SaqAI TTAA 7 cut(s) 435, 1941, 1994, 3452, 3584, 4302, 4455
Sau96I GGNCC 3 cut(s) 1186, 3075, 4271
ScaI AGTACT 1 cut(s) 2091
SchI GAGTC 4 cut(s) 593, 1157, 3727, 4428
ScrFI CCNGG 4 cut(s) 63, 1059, 1501, 4029
SduI GDGCHC 6 cut(s) 2527, 2965, 3276, 3897, 4503, 4547
SfcI CTRYAG 2 cut(s) 562, 1143
Sfr274I CTCGAG 1 cut(s) 2422
SfuI TTCGAA 2 cut(s) 2790, 3049
SinI GGWCC 3 cut(s) 1186, 3075, 4271
SlaI CTCGAG 1 cut(s) 2422
SmiMI CAYNNNNRTG 6 cut(s) 850, 1502, 1676, 2241, 3032, 4632
SmlI CTYRAG 3 cut(s) 2422, 3998, 4210
SmoI CTYRAG 3 cut(s) 2422, 3998, 4210
SnaBI TACGTA 1 cut(s) 671
SpeI ACTAGT 3 cut(s) 185, 1132, 4252
SphI GCATGC 3 cut(s) 2383, 3621, 4286
SspI AATATT 1 cut(s) 3257
SstI GAGCTC 1 cut(s) 3276
StyD4I CCNGG 4 cut(s) 61, 1057, 1499, 4027
StyI CCWWGG 5 cut(s) 6, 411, 2142, 2188, 3853
TaaI ACNGT 6 cut(s) 733, 1658, 1723, 2185, 2611, 3972
TaiI ACGT 9 cut(s) 366, 673, 1033, 1110, 1704, 2585, 2808, 3313, 4569
TaqII GACCGA 1 cut(s) 1203
TatI WGTACW 6 cut(s) 132, 979, 1077, 2089, 3639, 3807
TauI GCSGC 1 cut(s) 410
TfiI GAWTC 8 cut(s) 11, 50, 239, 1757, 2010, 2339, 2558, 2603
Tru1I TTAA 7 cut(s) 435, 1941, 1994, 3452, 3584, 4302, 4455
Tru9I TTAA 7 cut(s) 435, 1941, 1994, 3452, 3584, 4302, 4455
TscAI CASTG 8 cut(s) 326, 1844, 1897, 1985, 2188, 3876, 4406, 4522
TseFI GTSAC 6 cut(s) 1468, 1504, 2179, 2685, 3311, 3781
TseI GCWGC 9 cut(s) 416, 489, 564, 1550, 1988, 2675, 2678, 2856, 3614
Tsp45I GTSAC 6 cut(s) 1468, 1504, 2179, 2685, 3311, 3781
TspGWI ACGGA 4 cut(s) 195, 605, 2057, 4381
TspRI CASTG 8 cut(s) 326, 1844, 1897, 1985, 2188, 3876, 4406, 4522
Tth111I GACNNNGTC 1 cut(s) 4418
Van91I CCANNNNNTGG 2 cut(s) 1409, 2512
VpaK11BI GGWCC 3 cut(s) 1186, 3075, 4271
XbaI TCTAGA 3 cut(s) 2993, 4150, 4243
XceI RCATGY 4 cut(s) 2240, 2383, 3621, 4286
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 1218
XhoI CTCGAG 1 cut(s) 2422
XmiI GTMKAC 6 cut(s) 587, 1129, 1142, 1151, 3307, 4238
XmnI GAANNNNTTC 5 cut(s) 342, 750, 993, 2570, 3327
ZraI GACGTC 1 cut(s) 2583
ZrmI AGTACT 1 cut(s) 2091
Zsp2I ATGCAT 2 cut(s) 887, 3674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.