FvH4_3g44000

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000085
Physical Location & Seq
Reverse (-)
36689573 .. 36696555
6983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44000.t2

Sequence Viewer

Length: 4578 bp
ATGGCGCTTTTCAGAAGGTTCTTTAATCGGAAGCCGCCGGATCGGCTTCTAGAGATTTCTCAACGGGTTTACGTTTTTGACTGCTGCTTCTCCACCGATGTGTTGGAAGAAGATGAGTACAAAGTGTACTTGGGTGGCATTACAGCCCAGCTACAGGACTACTTCCCTGATGCTTCTTTCATGGTGTTTAACTTCAGAGAAGGGGATAAACGGAGCCAAATTTCAGATGTGTTGTCCCAGTATGACATGACAGTTATGGATTACCCTCGGCAATATGAGAATTGTCCACTGCTGCCACTGGAGATGATTCATCACTTTCTTCGATCAAGTGAAGGTTGGTTGTCATTGGCGGGACAACAGAATGTACTGCTGATGCACTGTGAAAGAGGAGGATGGCCCGTGCTTGCCTTTATGCTTGCAGCTCTTTTGCTATACCGTAAAACTTACACAGGGGAGCAGAAGACTCTTGATCTGGTTTACAAGCAGGCTCCAAAAGAGCTTCTCCATCTGTTGGCTTCTATAAACCCACAACCTTCGCAGCTTAGATATCTTCAGTATATTTCACGAAGAAATCCGGGTGCTGATTGGCTTCCATCAGATACACCTCTATTTTTGGATTGTCTGATACTTAGAAACCTTCCACTTTTTGATGACAGAAAAGGTTGCAGGCCCTTCATACGAGTTTATGGTCAAGACCCTTCAACACCGGCTGACAGAAGTTCTAAGCTTCTCTTTTCAAATTTAAAGACTAAAAACAATACTCGCCACTATTCACAGGCAGAGTGTGTGCTGGTGAAAATGGATATCTGTTGTTGTGTTCAAGGGGATGTGGTAGTCGAGTGTATCCACCTGTATGAAGATCAGGTTCATGAGGAGATGATGTTCAGAGTTATGTTTCACACAGCATTTGTAAGGTCAAATATATTGATGCTCAGCCGTGAAGATATTGATATTCTTTGGGATGCCAAGGAACAGTTCTCAAAAGACTTCAGATCAGAGGTACTTTTTTCGGACGCTGATGCTGTTCTGCCTAATCTCACCACAGTTGTGGCAAGTGATGATGGAAATGAGACAGAGACTGCTTCACCTGAGCAATCTTATGAGGTGGAAGAAATCTTCAGCGATGCTACTATCGAAATTGAGCCTGCAATGTCAGAGTCAAATGAAGTAGTTAAATTAAGAATACGAGCTACTAGTGAAACTGAGCCTACAATACCAAAGTTGACTGAAGATTTTGAACCAGTTGAAGCAACAACACAAGCCACCAATGGACACTGTTCAGGTGAACCAGCTCCTCAGTGGAAGCATGATGTGTTTTTAAGTTTTAGGGGTGAAGATACTCGCAAGGGTTTCCTCTCCCATTTATACCATGAATTGCAAACCACAAAAATGATTAGAACATTTAAGGATGATGAACTACTTAAGAAAGGGAAGGTTATTTCTCAAAGCCTGGTGACGGCAATTGAAGAATCAAGGTTTGCGATCATTGTTCTGTCAGAGCACTATGCTTTTTCTGCATGGTGTTTAGATGAACTTACAAAGATCTTTCAATGCATGGAAGGCAAGGATACAATCCTGCCAATTTTTTATCATGTGGATCCCTGTGATGTACGATATCAAAAGAATAGTTATGAAGTGGCCTTAACAAAGCATGAAAGCAAGGCGAGACATGACATAGAGAAGGTGAAGCAGTGGAGAGCTGATTTAACAAAAGTGGGAAATCTCTCTGGGTGGGATTCAAAGAACCACAAATCTGAAAGGGAACTAGTTGAAGACATTGTGAAAGTTGTGCTCAAAGAAGTACTTCAAGTACCAAGGGGAGATTTTGAAGAATTTGAAGCAACAAGAGAAGCCATGGTTGAGGTTAACAAGATGCTGAAGGATGATAAGATCACCGCCATTGGAGTGTATGGAAGAGGAGGCGTTGGCAAGACAGCCATGGTGAAACATGTCGGTGCACAAGCCAAAAGAAATGGGCTTTTCGATCATGTTGTTATAGCCGTTATATCACGAAACCCTGATTTGAAAAAAATTCAAGGGGCATTGGTAAATCAGCTGGGAGTGGAATTGGTGGAGGATGAAGAATTAGAAAGAGCCAGCAGATTGAAGGAGAGGATCATGGGAGGAAGGAGGATCCTAATTATCTTGGATGACATTTGGGAGAAAATAGATTTGTCAAGCATTGGAATTCCTAGCTATGACGAGCTTCAAAGATGCAATTCCAAGGTCCTACTCACCACAAGGATCAGGAATCTTTGTCATTCTATGGGGTGCCAAGAACCTATTCCTCTCAATATCCTATCAGAGGAAGATTCTTTGAAGCTATTCATGAAGAAGTCGAGAAAGCCTTTTCATGGATCTACCGATTCCTATGATGAGGAGAGGAGCGTAGTTGGGCAATGCGCTGGTCTCCCAAGTGTATTGGAACAGGCTGCAAAGGCATTTGGACATAAATACATGGCAGAATGGAATACAGCAGTTTCAAAGGCATTTGGAGATATGTCAGATAATGATGATGAATTGCAAAAAGCAGCTCGTGAAGTAAATGCATCTCAACTTGTTCCCACTAATAAGGATGAGAGAGATACGTTTAAAAGGATACAATTAACCTATAATACCTTGAGATTTGATGATGCCAAGTCATTTTTCTTGCTTTGCTGCATGTTTCCAGAAGATTATGATATTCCAATTGAAAAATTGTTGAAGTACAGCATTGGAAAAGGTTTGCTTCACTATTCTGACACGCTGCAAGAAGCTAGAGCCAGAGCACATTCTTTGATAGCATACCTTCAAGCTTCAGGGTTGCTTTTGGATGGCAAGAAAGGATGCATAAAAATGCATTGTGAGATCCGAAATGTGGCCATGTCTTTTGCGTTGTCTGAAGAAGGTCATGCTTTTTTCATAAAAGCTGGTTGTGATTTGAAGGATTGGCCAATGGATGCTCATGAAGGCTACTCGGCAATCTCACTAATGGGGAACAATCTTAACAAGCTTCCTGAAACTTTGGTATGTTCTGAACTCCAAATTTTACTGCTGCAAAATAATGATGGTATATCTGAAATTCCAGAAACTTTTTTTCAAAGTCCAAATGCACTAAGGGTCTTTGATCTTAGCTATACTGGTATTTCAGTACTACCCTCTTCATTCAATCTCCTCACCAACCTCCAAGCCTTGCATTTGGATTGTTGTAATAGAATAACTGACATATCCGTACTTGGGAAGCTGAAGAAAGTTGAGATTCTTAGTTTGAGAAAATTTCCTCATAAGGATTTGCCGAAAGAGATAGGAAATTTGACCAGCCTAAGGATGCTGGATGTCACTGGTGGACGTGTTCACAAAATTCCATCTAAAGTGATATCGAGGTTGTATAGACTGGAAGAATTGTATATGAGATGTGAATTTGGTGATTGGGGAAGTAAAGTCGAGGAAGCAGTAGGAGAAACAAATGCCGACTTTGATGAGATAATTGGCTTGTCCCATCTAAACATTTTGAAGGTTTACATATCTGATGCCGCTTGCTTACCCATAGATATTGGGAACTTTGAGCTAAATTGGATAAAATTTGACATATGCATCAGCAGATATTTCTCATCAGAGTTGATGCTTTCTTCAAATGAGCAACCTTCAAGAACCTTGACTCTTGACAGAACAATCAATGGCTTATCGGATTGGTTTATCAACATAGTGACCGAGAAAGCAGAAAGACTAGAGTACAGAGAGGGTAAGGGATTAAACAACATTCTTGAGGAATATGAGCATGGGAGATTACATGGGCTGAAACATCTCTCTTTAATTGGGTGCCATGAGAACTTGGAAGGGTTGATAGATGCAACAACTTGGGATCCAAGTAAACCTGTGTTCGAGAGCTTGGAGGAGTTACATCTCCTTCACTTGCAATGCCTCAAAGAGTTATGTGTTGGCGAATTGCCAGTTGGGTCTCTATGCAAGCTTAAGTTGTTGAAAGTGCAAACTTGTCGTAACTTGGTAAATGCACTTTTGCCACCAAAATTGTTGCAGAGACTAACAAATCTAGAAACCATATTTTGTGTAAATATGGAACACATGAAGTATTTGTTTGGATCTGAAGGGCTTGACCTCGGAACGATTCTTTTGACAAAATTGAGTGAGATGAGATTGTGTGCTCTGTTCGAACTCATAAGCATCTGGAATGGTCCCGCTCCCCGTGCAGTCTTTGGGAATCTTAAAGTTTTGGCAGTCTGCAAGTGTACAAAACTGAAAATTCTATTCCCATTTGATGTAGCTCAGTCTCTTGTGCAATTGGAAGACCTCTCTATAGAGCGCTGCCCCAACTTGGCTAGAGTTATTGAAGCAAGCAACAGCTTCGACAGATATATCTTTTTTCCACAATTGAAGAAATTAGCTATGATAAATCTTTCAGAGCTAACATGGTTCTGCACTGAAAATGGTTCTATGGGTATCGAGTGCCCTCTATTGGAATACTTGTACGTGGATGAGTGCCCCCAGTTCAAAAACACTGCTTCTGCTTCTGTTATACTCAATGATCAACAGCATTTGAATTCTCTAGCAAAAAGATGGGAGGATATGCATAAGCTTTAA

Protein Analysis

1526

Amino Acids

173.86

Weight (kDa)

5.51

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTEN_C2 PF10409 199 - 338 2e-36 C2 domain of PTEN tumour-suppressor protein
TIR PF01582 433 - 602 5.6e-49 TIR domain
TIR_2 PF13676 436 - 547 4.3e-13 TIR domain
NB-ARC PF00931 620 - 778 7.7e-29 NB-ARC domain
LRR_8 PF13855 1004 - 1060 2.4e-07 Leucine rich repeat
LRR_14 PF23598 1066 - 1129 6e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 1288 - 1433 4.2e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2271, 3798
AccB7I CCANNNNNTGG 1 cut(s) 511
AccBSI CCGCTC 1 cut(s) 4178
AciI CCGC 5 cut(s) 35, 350, 1896, 3513, 4176
AcoI YGGCCR 2 cut(s) 2859, 2930
AfeI AGCGCT 1 cut(s) 4301
AflII CTTAAG 2 cut(s) 1421, 3950
AflIII ACRYGT 2 cut(s) 1948, 3328
AhlI ACTAGT 2 cut(s) 1193, 1765
AjiI CACGTC 1 cut(s) 3329
AjnI CCWGG 1 cut(s) 1449
AjuI GAANNNNNNNTTGG 8 cut(s) 959, 991, 2268, 2300, 2630, 2662, 3915, 3947
AleI CACNNNNGTG 2 cut(s) 98, 1046
AloI GAACNNNNNNTCC 2 cut(s) 866, 898
Alw21I GWGCWC 5 cut(s) 1503, 1794, 1960, 2770, 4144
Alw26I GTCTC 7 cut(s) 1064, 1070, 1660, 2414, 3942, 4012, 4272
Alw44I GTGCAC 1 cut(s) 1956
AlwNI CAGNNNCTG 1 cut(s) 1079
Aor51HI AGCGCT 1 cut(s) 4301
AoxI GGCC 5 cut(s) 395, 668, 1638, 2859, 2930
ApaLI GTGCAC 1 cut(s) 1956
Asp700I GAANNNNTTC 3 cut(s) 1803, 2283, 2324
AspLEI GCGC 3 cut(s) 7, 2405, 4302
AspS9I GGNCC 4 cut(s) 396, 669, 2227, 4172
AsuC2I CCSGG 1 cut(s) 576
AsuII TTCGAA 1 cut(s) 4149
AvaII GGWCC 2 cut(s) 2227, 4172
AxyI CCTNAGG 1 cut(s) 3302
BaeGI GKGCMC 3 cut(s) 1960, 4448, 4481
BalI TGGCCA 2 cut(s) 2861, 2932
BamHI GGATCC 3 cut(s) 1597, 2133, 3841
BanI GGYRCC 2 cut(s) 2271, 3798
BauI CACGAG 1 cut(s) 2535
BbsI GAAGAC 3 cut(s) 467, 1779, 4290
Bbv12I GWGCWC 5 cut(s) 1503, 1794, 1960, 2770, 4144
BccI CCATC 9 cut(s) 387, 513, 601, 1055, 2807, 3041, 3352, 3486, 4548
BceAI ACGGC 3 cut(s) 921, 1473, 1985
BcgI CGANNNNNNTGC 4 cut(s) 3956, 3990, 4324, 4358
BciT130I CCWGG 1 cut(s) 1451
BciVI GTATCC 3 cut(s) 854, 1561, 2592
BclI TGATCA 1 cut(s) 4522
BcnI CCSGG 1 cut(s) 576
BcoDI GTCTC 7 cut(s) 1064, 1070, 1660, 2414, 3942, 4012, 4272
BcuI ACTAGT 2 cut(s) 1193, 1765
BfaI CTAG 9 cut(s) 50, 1194, 1766, 2193, 2757, 3707, 4031, 4317, 4544
BfmI CTRYAG 2 cut(s) 152, 4293
BfoI RGCGCY 2 cut(s) 8, 4303
BfrI CTTAAG 2 cut(s) 1421, 3950
BfuI GTATCC 3 cut(s) 854, 1561, 2592
BglI GCCNNNNNGGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 1542
BlpI GCTNAGC 1 cut(s) 932
BmcAI AGTACT 2 cut(s) 1803, 3132
Bme1390I CCNGG 2 cut(s) 576, 1451
Bme18I GGWCC 2 cut(s) 2227, 4172
BmgBI CACGTC 1 cut(s) 3329
BmgT120I GGNCC 4 cut(s) 396, 669, 2227, 4172
BmiI GGNNCC 8 cut(s) 215, 489, 1599, 2135, 2273, 3800, 3843, 4174
BmrFI CCNGG 2 cut(s) 576, 1451
BmrI ACTGGG 2 cut(s) 232, 4477
BmuI ACTGGG 2 cut(s) 232, 4477
BpiI GAAGAC 3 cut(s) 467, 1779, 4290
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu1102I GCTNAGC 1 cut(s) 932
Bpu14I TTCGAA 1 cut(s) 4149
BpuEI CTTGAG 2 cut(s) 2641, 3764
BpuMI CCSGG 1 cut(s) 576
BsaAI YACGTR 1 cut(s) 4468
BsaI GGTCTC 2 cut(s) 2414, 3942
BsaJI CCNNGG 7 cut(s) 266, 966, 1814, 1854, 1938, 2223, 4096
BsaXI ACNNNNNCTCC 2 cut(s) 866, 896
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 8 cut(s) 238, 303, 1241, 3124, 3325, 3378, 3929, 4483
Bse21I CCTNAGG 1 cut(s) 3302
Bse3DI GCAATG 3 cut(s) 1155, 2405, 3902
BseBI CCWGG 1 cut(s) 1451
BseDI CCNNGG 7 cut(s) 266, 966, 1814, 1854, 1938, 2223, 4096
BseMI GCAATG 3 cut(s) 1155, 2405, 3902
BseMII CTCAG 5 cut(s) 946, 1080, 1194, 1310, 4277
BseNI ACTGG 8 cut(s) 238, 303, 1241, 3124, 3325, 3378, 3929, 4483
BseRI GAGGAG 8 cut(s) 402, 887, 1284, 1932, 2393, 2398, 3143, 3887
BseSI GKGCMC 3 cut(s) 1960, 4448, 4481
BseYI CCCAGC 2 cut(s) 147, 2056
BsgI GTGCAG 2 cut(s) 4206, 4399
BshFI GGCC 5 cut(s) 397, 670, 1640, 2861, 2932
BshNI GGYRCC 2 cut(s) 2271, 3798
BsiHKAI GWGCWC 5 cut(s) 1503, 1794, 1960, 2770, 4144
BsiSI CCGG 3 cut(s) 38, 575, 707
BslFI GGGAC 4 cut(s) 220, 366, 3460, 4158
BsmAI GTCTC 7 cut(s) 1064, 1070, 1660, 2414, 3942, 4012, 4272
BsmFI GGGAC 4 cut(s) 220, 366, 3460, 4158
BsnI GGCC 5 cut(s) 397, 670, 1640, 2861, 2932
Bso31I GGTCTC 2 cut(s) 2414, 3942
Bsp119I TTCGAA 1 cut(s) 4149
Bsp1286I GDGCHC 7 cut(s) 1503, 1794, 1960, 2770, 4144, 4448, 4481
Bsp1407I TGTACA 1 cut(s) 4226
Bsp1720I GCTNAGC 1 cut(s) 932
Bsp19I CCATGG 2 cut(s) 1854, 1938
BspACI CCGC 5 cut(s) 35, 350, 1896, 3513, 4176
BspANI GGCC 5 cut(s) 397, 670, 1640, 2861, 2932
BspCNI CTCAG 5 cut(s) 945, 1081, 1195, 1309, 4276
BspHI TCATGA 3 cut(s) 868, 2328, 2944
BspLI GGNNCC 8 cut(s) 215, 489, 1599, 2135, 2273, 3800, 3843, 4174
BspT104I TTCGAA 1 cut(s) 4149
BspT107I GGYRCC 2 cut(s) 2271, 3798
BspTI CTTAAG 2 cut(s) 1421, 3950
BspTNI GGTCTC 2 cut(s) 2414, 3942
BsrBI CCGCTC 1 cut(s) 4178
BsrDI GCAATG 3 cut(s) 1155, 2405, 3902
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 4226
BsrI ACTGG 8 cut(s) 238, 303, 1241, 3124, 3325, 3378, 3929, 4483
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 7 cut(s) 266, 966, 1814, 1854, 1938, 2223, 4096
BssSI CACGAG 1 cut(s) 2535
BssT1I CCWWGG 5 cut(s) 966, 1814, 1854, 1938, 2223
Bst2BI CACGAG 1 cut(s) 2535
Bst2UI CCWGG 1 cut(s) 1451
Bst4CI ACNGT 6 cut(s) 253, 380, 437, 975, 1045, 1277
Bst6I CTCTTC 2 cut(s) 1909, 3145
BstAFI CTTAAG 2 cut(s) 1421, 3950
BstAUI TGTACA 1 cut(s) 4226
BstBAI YACGTR 1 cut(s) 4468
BstBI TTCGAA 1 cut(s) 4149
BstC8I GCNNGC 9 cut(s) 405, 417, 486, 668, 1146, 2098, 3517, 3947, 4333
BstDSI CCRYGG 2 cut(s) 1854, 1938
BstENI CCTNNNNNAGG 1 cut(s) 2303
BstH2I RGCGCY 2 cut(s) 8, 4303
BstHHI GCGC 3 cut(s) 7, 2405, 4302
BstMAI GTCTC 7 cut(s) 1064, 1070, 1660, 2414, 3942, 4012, 4272
BstMWI GCNNNNNNNGC 5 cut(s) 43, 1514, 1560, 2438, 4184
BstNI CCWGG 1 cut(s) 1451
BstNSI RCATGY 2 cut(s) 1952, 2665
BstSCI CCNGG 2 cut(s) 574, 1449
BstSFI CTRYAG 2 cut(s) 152, 4293
BstSLI GKGCMC 3 cut(s) 1960, 4448, 4481
BstV2I GAAGAC 3 cut(s) 467, 1779, 4290
BstX2I RGATCY 7 cut(s) 1542, 1597, 2133, 2357, 2847, 3841, 4079
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstYI RGATCY 7 cut(s) 1542, 1597, 2133, 2357, 2847, 3841, 4079
Bsu36I CCTNAGG 1 cut(s) 3302
BsuI GTATCC 3 cut(s) 854, 1561, 2592
BsuRI GGCC 5 cut(s) 397, 670, 1640, 2861, 2932
BtgI CCRYGG 2 cut(s) 1854, 1938
BtgZI GCGATG 1 cut(s) 1137
BtrI CACGTC 1 cut(s) 3329
BtsI GCAGTG 3 cut(s) 287, 1697, 4494
BtsIMutI CAGTG 9 cut(s) 287, 296, 376, 1273, 1304, 1697, 3318, 4416, 4494
Cac8I GCNNGC 9 cut(s) 405, 417, 486, 668, 1146, 2098, 3517, 3947, 4333
CaiI CAGNNNCTG 1 cut(s) 1079
CciI TCATGA 3 cut(s) 868, 2328, 2944
CfoI GCGC 3 cut(s) 7, 2405, 4302
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 4 cut(s) 396, 669, 2227, 4172
CseI GACGC 1 cut(s) 1022
CspCI CAANNNNNGTGG 2 cut(s) 3990, 4025
DraI TTTAAA 2 cut(s) 744, 2593
EaeI YGGCCR 2 cut(s) 2859, 2930
Eam1104I CTCTTC 2 cut(s) 1909, 3145
EarI CTCTTC 2 cut(s) 1909, 3145
Eco130I CCWWGG 5 cut(s) 966, 1814, 1854, 1938, 2223
Eco31I GGTCTC 2 cut(s) 2414, 3942
Eco32I GATATC 4 cut(s) 548, 805, 1616, 3357
Eco47I GGWCC 2 cut(s) 2227, 4172
Eco47III AGCGCT 1 cut(s) 4301
Eco81I CCTNAGG 1 cut(s) 3302
EcoNI CCTNNNNNAGG 1 cut(s) 2303
EcoO109I RGGNCCY 2 cut(s) 669, 2227
EcoRI GAATTC 2 cut(s) 2187, 4537
EcoRII CCWGG 1 cut(s) 1449
EcoRV GATATC 4 cut(s) 548, 805, 1616, 3357
EcoT14I CCWWGG 5 cut(s) 966, 1814, 1854, 1938, 2223
EcoT22I ATGCAT 6 cut(s) 1556, 2551, 2831, 2841, 3575, 4569
ErhI CCWWGG 5 cut(s) 966, 1814, 1854, 1938, 2223
FalI AAGNNNNNCTT 6 cut(s) 716, 748, 1788, 1820, 2712, 2744
FaqI GGGAC 4 cut(s) 220, 366, 3460, 4158
FauI CCCGC 2 cut(s) 343, 4183
FauNDI CATATG 1 cut(s) 3569
FbaI TGATCA 1 cut(s) 4522
FspBI CTAG 9 cut(s) 50, 1194, 1766, 2193, 2757, 3707, 4031, 4317, 4544
GlaI GCGC 3 cut(s) 6, 2404, 4301
GsaI CCCAGC 2 cut(s) 151, 2060
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 2 cut(s) 8, 4303
HaeIII GGCC 5 cut(s) 397, 670, 1640, 2861, 2932
HapII CCGG 3 cut(s) 38, 575, 707
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 3 cut(s) 7, 2405, 4302
Hin6I GCGC 3 cut(s) 5, 2403, 4300
HinP1I GCGC 3 cut(s) 5, 2403, 4300
HincII GTYRAC 2 cut(s) 1224, 1867
HindII GTYRAC 2 cut(s) 1224, 1867
HindIII AAGCTT 5 cut(s) 725, 2793, 2990, 3947, 4571
HpaI GTTAAC 1 cut(s) 1867
HpaII CCGG 3 cut(s) 38, 575, 707
HpyCH4III ACNGT 6 cut(s) 253, 380, 437, 975, 1045, 1277
HpyCH4IV ACGT 4 cut(s) 72, 2588, 3328, 4467
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 1514, 1560, 2438, 4184
HpySE526I ACGT 4 cut(s) 72, 2588, 3328, 4467
HspAI GCGC 3 cut(s) 5, 2403, 4300
Ksp22I TGATCA 1 cut(s) 4522
KspAI GTTAAC 1 cut(s) 1867
LmnI GCTCC 6 cut(s) 213, 454, 493, 1297, 2385, 4183
MaeI CTAG 9 cut(s) 50, 1194, 1766, 2193, 2757, 3707, 4031, 4317, 4544
MaeII ACGT 4 cut(s) 72, 2588, 3328, 4467
MaeIII GTNAC 5 cut(s) 1453, 3316, 3685, 3876, 3977
MbiI CCGCTC 1 cut(s) 4178
MfeI CAATTG 4 cut(s) 1461, 2688, 4277, 4367
MflI RGATCY 7 cut(s) 1542, 1597, 2133, 2357, 2847, 3841, 4079
MhlI GDGCHC 7 cut(s) 1503, 1794, 1960, 2770, 4144, 4448, 4481
MlsI TGGCCA 2 cut(s) 2861, 2932
MluNI TGGCCA 2 cut(s) 2861, 2932
MlyI GAGTC 3 cut(s) 457, 1166, 3631
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 2 cut(s) 2861, 2932
Mph1103I ATGCAT 6 cut(s) 1556, 2551, 2831, 2841, 3575, 4569
MroXI GAANNNNTTC 3 cut(s) 1803, 2283, 2324
MscI TGGCCA 2 cut(s) 2861, 2932
MslI CAYNNNNRTG 7 cut(s) 98, 852, 1046, 1388, 1904, 1953, 2834
Msp20I TGGCCA 2 cut(s) 2861, 2932
MspA1I CMGCKG 1 cut(s) 2056
MspCI CTTAAG 2 cut(s) 1421, 3950
MspI CCGG 3 cut(s) 38, 575, 707
MspR9I CCNGG 2 cut(s) 576, 1451
MunI CAATTG 4 cut(s) 1461, 2688, 4277, 4367
MvaI CCWGG 1 cut(s) 1451
MwoI GCNNNNNNNGC 5 cut(s) 43, 1514, 1560, 2438, 4184
NciI CCSGG 1 cut(s) 576
NcoI CCATGG 2 cut(s) 1854, 1938
NdeI CATATG 1 cut(s) 3569
NlaIV GGNNCC 8 cut(s) 215, 489, 1599, 2135, 2273, 3800, 3843, 4174
NmeAIII GCCGAG 2 cut(s) 247, 2936
NmuCI GTSAC 3 cut(s) 1453, 3316, 3685
NsiI ATGCAT 6 cut(s) 1556, 2551, 2831, 2841, 3575, 4569
NspI RCATGY 2 cut(s) 1952, 2665
NspV TTCGAA 1 cut(s) 4149
OliI CACNNNNGTG 2 cut(s) 98, 1046
PagI TCATGA 3 cut(s) 868, 2328, 2944
PciI ACATGT 1 cut(s) 1948
PdmI GAANNNNTTC 3 cut(s) 1803, 2283, 2324
PfeI GAWTC 9 cut(s) 307, 1469, 1736, 2251, 2312, 2366, 3238, 4105, 4198
PflMI CCANNNNNTGG 1 cut(s) 511
PleI GAGTC 3 cut(s) 457, 1165, 3631
PpsI GAGTC 3 cut(s) 457, 1165, 3631
Ppu21I YACGTR 1 cut(s) 4468
PpuMI RGGWCCY 1 cut(s) 2227
PscI ACATGT 1 cut(s) 1948
Psp5II RGGWCCY 1 cut(s) 2227
Psp6I CCWGG 1 cut(s) 1449
PspFI CCCAGC 2 cut(s) 147, 2056
PspGI CCWGG 1 cut(s) 1449
PspN4I GGNNCC 8 cut(s) 215, 489, 1599, 2135, 2273, 3800, 3843, 4174
PspPI GGNCC 4 cut(s) 396, 669, 2227, 4172
PspPPI RGGWCCY 1 cut(s) 2227
PstNI CAGNNNCTG 1 cut(s) 1079
PsuI RGATCY 7 cut(s) 1542, 1597, 2133, 2357, 2847, 3841, 4079
PvuII CAGCTG 1 cut(s) 2056
RseI CAYNNNNRTG 7 cut(s) 98, 852, 1046, 1388, 1904, 1953, 2834
Sau96I GGNCC 4 cut(s) 396, 669, 2227, 4172
ScaI AGTACT 2 cut(s) 1803, 3132
SchI GAGTC 3 cut(s) 457, 1166, 3631
ScrFI CCNGG 2 cut(s) 576, 1451
SduI GDGCHC 7 cut(s) 1503, 1794, 1960, 2770, 4144, 4448, 4481
SfcI CTRYAG 2 cut(s) 152, 4293
SfuI TTCGAA 1 cut(s) 4149
SinI GGWCC 2 cut(s) 2227, 4172
SmiMI CAYNNNNRTG 7 cut(s) 98, 852, 1046, 1388, 1904, 1953, 2834
SmlI CTYRAG 4 cut(s) 1421, 2620, 3743, 3950
SmoI CTYRAG 4 cut(s) 1421, 2620, 3743, 3950
SpeI ACTAGT 2 cut(s) 1193, 1765
SsiI CCGC 5 cut(s) 35, 350, 1896, 3513, 4176
SspMI CTAG 9 cut(s) 50, 1194, 1766, 2193, 2757, 3707, 4031, 4317, 4544
StyD4I CCNGG 2 cut(s) 574, 1449
StyI CCWWGG 5 cut(s) 966, 1814, 1854, 1938, 2223
TaaI ACNGT 6 cut(s) 253, 380, 437, 975, 1045, 1277
TaiI ACGT 4 cut(s) 75, 2591, 3331, 4470
TaqII GACCGA 1 cut(s) 3704
TatI WGTACW 8 cut(s) 117, 126, 364, 1801, 2706, 3130, 3711, 4226
TauI GCSGC 2 cut(s) 37, 3515
TfiI GAWTC 9 cut(s) 307, 1469, 1736, 2251, 2312, 2366, 3238, 4105, 4198
TscAI CASTG 9 cut(s) 294, 303, 383, 1280, 1304, 1697, 3325, 4423, 4501
TseFI GTSAC 3 cut(s) 1453, 3316, 3685
Tsp45I GTSAC 3 cut(s) 1453, 3316, 3685
TspGWI ACGGA 2 cut(s) 226, 3199
TspRI CASTG 9 cut(s) 294, 303, 383, 1280, 1304, 1697, 3325, 4423, 4501
Van91I CCANNNNNTGG 1 cut(s) 511
Vha464I CTTAAG 2 cut(s) 1421, 3950
VneI GTGCAC 1 cut(s) 1956
VpaK11BI GGWCC 2 cut(s) 2227, 4172
XagI CCTNNNNNAGG 1 cut(s) 2303
XbaI TCTAGA 2 cut(s) 49, 4030
XceI RCATGY 2 cut(s) 1952, 2665
XcmI CCANNNNNNNNNTGG 2 cut(s) 100, 1296
XmnI GAANNNNTTC 3 cut(s) 1803, 2283, 2324
XspI CTAG 9 cut(s) 50, 1194, 1766, 2193, 2757, 3707, 4031, 4317, 4544
ZrmI AGTACT 2 cut(s) 1803, 3132
Zsp2I ATGCAT 6 cut(s) 1556, 2551, 2831, 2841, 3575, 4569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.