Rorug05G0362300

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000085
Physical Location & Seq
Forward (+)
47964890 .. 47971798
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0362300.1

Sequence Viewer

Length: 2742 bp
ATGATGATCACAAAGTTGGATAAAATGTCTGCTTCTTCTCCATCATGGGAGCGTGCCCAACGTTTGTTCGACAAGATTACTGAGTTGGAGAGTAGGCGTCGGAGGTCTGCCCAAGCACGAGTTCCCTCAGATCCTAATGCCTGGCAACAGATACGTGAGAATTATGAAGCTATAATTCTTGAGGACCATGCATTTTCAGAGCAGCACAATGTTGAGTATGCTTTGTGGCAGTTACATTACAAGCGGATTGACGAATTGCGCACGCACTTCAGTGCTGCTATGGCTTCTACAGGTTCAAACTCATCCCAAGGTTTGAAGGGACCTACTCGGCCTGATCGTATTACAAAAATAAGATTGCAGTTTAAAACATTCCTTTCAGAAGCGACTGGATTTTATCATGATTTAATAGTGAAGATCAGAGCAAAGTATGGGCTTCCTCTTGGCTATTTCTCTGAGGATTCTGAAAATCGAATTGTTATGGATAAGGATGGAAAGAAATCTGCTGATATGAAGAAAGGTTTGATATCATGCCACCGCTGTTTGATTTACTTGGGTGACCTTGCACGCTACAAAGGACTGTATGGGGAGGGGGACTCCAAAACTCGTGAGTATGCTGCTGCCTCAAGTTACTACTTGCAAGCTGCATCTTGTTTGCCATCAAGTGGGAACCCTCATCATCAGCTTGCTATATTGGCTTCTTATTCGGGGGATGAGTTGGTGACTGTTTATCGATATTTTCGTAGCCTGGCCGTGGATAATCCGTTTTCAACTGCAAGGGATAACTTGATTGTGGCATTTGAGAAGAATCGTCAGAGTTACTCACAACTGTCTGGCAATGCTAATGCTTCTGCACTCAAAGAGTTGCCTGCACGATTGACTGGCAAAGGTAGAGGGAAAGGGGAAGCAATACCTGCTAAAGATAATAACACAGAAGCAGGCCTTGTTAAGGAAAGATCATCCAGTAATCAGGAAAAATACAAAGCTTTTTCAATTCGTTTTGTCCGTCTAAATGGCATTCTTTTTACACGTACTAGCCTGGAGACATTTGCAGAAGTTCTCTCTGTTGTTAGCAGTGGTTTATATGAGCTTCTATCTTCTGGAGCAGAAGAGGAGCTGAGTCTTGGTGCTGATGCTGTTGAGAATGGACTGGTCATTGTTCGACTGGTCTCTATACTGATATTCACAGTTCATAATGTGAAAAAGGAAAGTGAAGGTCAGTCATATGCAGAAATTGTACAGCGTGCTGTTCTGCTGCAAAATGCATTTACTGCTGTCTTTGAGTTGATGGGGCATGTCTTAGAGAGATGCGTGAAGCTGGGGGATCCAACTTCAAGTCACCTGTTACCAGGCATTCTGGTTTTTGTAGAATGGTTGGCATGTTGTCCTGATGTAGCAGCTGGCAGTGATGCAGATGAAAAACAGTCATCTGTTAGAGCAAAGTTCTGGAATGCTTGCATACACTTGTTGAATAATCTCTTATCATCTGTTCCGATGTCCATAGATGATGATGGAGATGAGACTTGCTTTAATAATATGAGCAGATATGAAGAAGGGGAAACTGAGAATCGCCTTGCTTTGTGGGAGGATTTTGAGTTGAGAGGATTCATGCCACTTCTTCCAGCACAGACAATATTGGATTTCTCGAGGAAGCATTCTTTTGGAAGTGATGGCCAGAAGGAAAAAGGTGCGCGTCTTAAAAGGATCCTGGCGGCAGGCAAGGCTTTAGCAAATGTGGTCAAGGTTGATCAAAAAGCCATTTATTTTGATTCCCAGACAAAGAAATTTGTCATTGGTGTTGAGCCTTCAATGAATGGTGATTATATGCCTACCTCCTTTTTAGGCATGGCTAATTCCAATGATAATCTGGAAGAAAATCAAGTGGAGGACACCAAAAATCTGGGAGGTCCATTTCAAAAGCCAGAGTTGACCATGGATGGGGATGAGGAAGATGAAGTAATAGTTTTTAAACCAATAGTGGCTGAGAAGAGGCCTGATGTGGTAGGCACTACATGGGCTATCCCCCAGCATTTGGAACCATTTAAATCAGAAGAAATATCTCTTGCTAACAACCTAAAGAGTTTGGGATTTATGGGCAATGGGCAAGTACTGAAATCTGACCATGTATCAAGTTCGGTTCCATTCCAACAGCCTGTTAATGGTAGTACTAGTAGTATGTTTTACAGCCATGCAAAGGCTCCAGAAGCTGTGTTGCCGTTTAAGGTTGATACTATTGCATCTTCTGGACCATTTGCGGATGGTTTGACATTGAAGACGTCATCAGCTTTGCCCGCTGCTATCCGAAAAAATCCAGTTAGTCGACCTGTTAGACACCTTGGTCCGCCACCTGGTTTTAGCCATGTTCCCGCTAAACAAGTGAACGAGTCTATTTATAATTCAGAGTCAATGGGTGAGAATCCACTGATGGATGATTACAGCTGGTTGGATGGGTATCAGGTGCCATCTTCAACTAAAGGAAATGCATTCAGCAGTTCCATTAACTACTCATCTCATTCAAATCTCCATCGCGTTCCTAATGGGAATGGCTTAAGTGGAACAGTAAACTTTCCCTTTCCTGGTAAACAGGGCCCATCTATGCCATTCCAAGCAGAAAACCAGAAAAGCCGGCAAGAATTCCATATGCTCGATGATCTTAAACTTCACCATGAAATGCAGCTGCAGCAGCAGCAACAACCTGAGCAATATCAAGGACAGTCTGTCTGGACAGGCCGCTATTTTGTGTGA

Protein Analysis

913

Amino Acids

101.41

Weight (kDa)

6.19

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EST1 PF10374 71 - 194 2.5e-17 Telomerase activating protein Est1
EST1_DNA_bind PF10373 207 - 540 4.2e-64 Est1 DNA/RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2391
AasI GACNNNNNNGTC 1 cut(s) 2334
AatII GACGTC 1 cut(s) 2276
Acc16I TGCGCA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 2455
AccB7I CCANNNNNTGG 2 cut(s) 662, 2029
AccI GTMKAC 1 cut(s) 2317
AccII CGCG 2 cut(s) 1690, 2526
AciI CCGC 8 cut(s) 244, 535, 1709, 2252, 2289, 2339, 2364, 2728
AclI AACGTT 1 cut(s) 61
AclWI GGATC 5 cut(s) 125, 1316, 1329, 1696, 1709
AcoI YGGCCR 2 cut(s) 747, 1669
AcsI RAATTY 2 cut(s) 1781, 2630
AcuI CTGAAG 1 cut(s) 253
AcyI GRCGYC 2 cut(s) 97, 2273
AfaI GTAC 4 cut(s) 1030, 1236, 2106, 2164
AfiI CCNNNNNNNGG 9 cut(s) 662, 751, 946, 1935, 2029, 2156, 2191, 2345, 2573
AflII CTTAAG 1 cut(s) 2545
AflIII ACRYGT 1 cut(s) 1025
AhdI GACNNNNNGTC 1 cut(s) 2714
AhlI ACTAGT 1 cut(s) 2165
AjnI CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AleI CACNNNNGTG 1 cut(s) 270
Alw26I GTCTC 3 cut(s) 1034, 1171, 1511
AlwI GGATC 5 cut(s) 125, 1316, 1329, 1696, 1709
AlwNI CAGNNNCTG 1 cut(s) 2204
Ama87I CYCGRG 1 cut(s) 1642
AoxI GGCC 7 cut(s) 329, 747, 937, 1669, 1988, 2584, 2725
ApaI GGGCCC 1 cut(s) 2588
ApoI RAATTY 2 cut(s) 1781, 2630
AspLEI GCGC 2 cut(s) 261, 1690
AspS9I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
AsuHPI GGTGA 6 cut(s) 566, 730, 1328, 1826, 2420, 2651
AvaI CYCGRG 1 cut(s) 1642
AvaII GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
BaeGI GKGCMC 2 cut(s) 58, 2588
BalI TGGCCA 1 cut(s) 1671
BamHI GGATCC 2 cut(s) 1321, 1701
BanI GGYRCC 1 cut(s) 2455
BanII GRGCYC 1 cut(s) 2588
BauI CACGAG 2 cut(s) 117, 603
BbsI GAAGAC 1 cut(s) 2276
BceAI ACGGC 2 cut(s) 734, 2197
BciT130I CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BclI TGATCA 2 cut(s) 6, 1744
BcoDI GTCTC 3 cut(s) 1034, 1171, 1511
BcuI ACTAGT 1 cut(s) 2165
BfaI CTAG 2 cut(s) 1032, 2166
BfmI CTRYAG 2 cut(s) 288, 2675
BfrI CTTAAG 1 cut(s) 2545
BfuAI ACCTGC 1 cut(s) 919
BmcAI AGTACT 2 cut(s) 2106, 2164
Bme1390I CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
Bme18I GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
BmeRI GACNNNNNGTC 1 cut(s) 2714
BmeT110I CYCGRG 1 cut(s) 1642
BmgT120I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
BmiI GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
BmrFI CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BmsI GCATC 5 cut(s) 653, 1120, 1295, 1396, 2243
BpiI GAAGAC 1 cut(s) 2276
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BpmI CTGGAG 3 cut(s) 1058, 1119, 2181
Bpu10I CCTNAGC 1 cut(s) 2694
BpuEI CTTGAG 2 cut(s) 200, 607
Bsa29I ATCGAT 1 cut(s) 730
BsaAI YACGTR 2 cut(s) 155, 1028
BsaBI GATNNNNATC 1 cut(s) 2448
BsaHI GRCGYC 2 cut(s) 97, 2273
BsaI GGTCTC 1 cut(s) 1171
BsaJI CCNNGG 4 cut(s) 307, 750, 1929, 2332
Bsc4I CCNNNNNNNGG 9 cut(s) 662, 751, 946, 1935, 2029, 2156, 2191, 2345, 2573
Bse118I RCCGGY 1 cut(s) 2622
Bse1I ACTGG 6 cut(s) 391, 883, 960, 1152, 1167, 2309
Bse3DI GCAATG 2 cut(s) 841, 2101
Bse8I GATNNNNATC 1 cut(s) 2448
BseBI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BseCI ATCGAT 1 cut(s) 730
BseDI CCNNGG 4 cut(s) 307, 750, 1929, 2332
BseGI GGATG 9 cut(s) 302, 493, 715, 956, 1939, 1945, 2260, 2431, 2449
BseJI GATNNNNATC 1 cut(s) 2448
BseLI CCNNNNNNNGG 9 cut(s) 662, 751, 946, 1935, 2029, 2156, 2191, 2345, 2573
BseMI GCAATG 2 cut(s) 841, 2101
BseMII CTCAG 7 cut(s) 72, 141, 444, 1106, 1551, 1971, 2685
BseNI ACTGG 6 cut(s) 391, 883, 960, 1152, 1167, 2309
BseRI GAGGAG 1 cut(s) 1124
BseSI GKGCMC 2 cut(s) 58, 2588
BseYI CCCAGC 2 cut(s) 1315, 2022
BsgI GTGCAG 2 cut(s) 834, 852
Bsh1236I CGCG 2 cut(s) 1690, 2526
BshFI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
BshNI GGYRCC 1 cut(s) 2455
BshVI ATCGAT 1 cut(s) 730
BsiHKCI CYCGRG 1 cut(s) 1642
BsiSI CCGG 1 cut(s) 2623
BslFI GGGAC 2 cut(s) 333, 605
BslI CCNNNNNNNGG 9 cut(s) 662, 751, 946, 1935, 2029, 2156, 2191, 2345, 2573
BsmAI GTCTC 3 cut(s) 1034, 1171, 1511
BsmFI GGGAC 2 cut(s) 333, 605
BsmI GAATGC 5 cut(s) 1014, 1350, 1453, 1651, 2480
BsnI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
Bso31I GGTCTC 1 cut(s) 1171
BsoBI CYCGRG 1 cut(s) 1642
Bsp120I GGGCCC 1 cut(s) 2584
Bsp1286I GDGCHC 2 cut(s) 58, 2588
Bsp1407I TGTACA 1 cut(s) 1234
Bsp143I GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
Bsp19I CCATGG 1 cut(s) 1929
BspACI CCGC 8 cut(s) 244, 535, 1709, 2252, 2289, 2339, 2364, 2728
BspANI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
BspCNI CTCAG 7 cut(s) 73, 140, 445, 1107, 1552, 1972, 2686
BspDI ATCGAT 1 cut(s) 730
BspFNI CGCG 2 cut(s) 1690, 2526
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
BspMAI CTGCAG 1 cut(s) 2679
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 5 cut(s) 125, 1316, 1329, 1696, 1709
BspT107I GGYRCC 1 cut(s) 2455
BspTI CTTAAG 1 cut(s) 2545
BspTNI GGTCTC 1 cut(s) 1171
BsrDI GCAATG 2 cut(s) 841, 2101
BsrFI RCCGGY 1 cut(s) 2622
BsrGI TGTACA 1 cut(s) 1234
BsrI ACTGG 6 cut(s) 391, 883, 960, 1152, 1167, 2309
BssAI RCCGGY 1 cut(s) 2622
BssECI CCNNGG 4 cut(s) 307, 750, 1929, 2332
BssMI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
BssNI GRCGYC 2 cut(s) 97, 2273
BssSI CACGAG 2 cut(s) 117, 603
BssT1I CCWWGG 3 cut(s) 307, 1929, 2332
Bst2BI CACGAG 2 cut(s) 117, 603
Bst2UI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
Bst4CI ACNGT 7 cut(s) 579, 724, 828, 1186, 1422, 2557, 2712
Bst6I CTCTTC 2 cut(s) 1101, 1979
BstACI GRCGYC 2 cut(s) 97, 2273
BstAFI CTTAAG 1 cut(s) 2545
BstAPI GCANNNNNTGC 2 cut(s) 911, 1268
BstAUI TGTACA 1 cut(s) 1234
BstBAI YACGTR 2 cut(s) 155, 1028
BstDEI CTNAG 8 cut(s) 81, 127, 453, 1115, 1297, 1560, 1980, 2694
BstDSI CCRYGG 2 cut(s) 750, 1929
BstEII GGTNACC 1 cut(s) 554
BstENI CCTNNNNNAGG 1 cut(s) 944
BstF5I GGATG 9 cut(s) 302, 493, 715, 956, 1939, 1945, 2260, 2431, 2449
BstFNI CGCG 2 cut(s) 1690, 2526
BstHHI GCGC 2 cut(s) 261, 1690
BstKTI GATC 9 cut(s) 9, 133, 337, 417, 956, 1324, 1704, 1747, 2650
BstMAI GTCTC 3 cut(s) 1034, 1171, 1511
BstMBI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
BstNI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
BstNSI RCATGY 2 cut(s) 1295, 1380
BstPI GGTNACC 1 cut(s) 554
BstSCI CCNGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
BstSFI CTRYAG 2 cut(s) 288, 2675
BstSLI GKGCMC 2 cut(s) 58, 2588
BstUI CGCG 2 cut(s) 1690, 2526
BstV2I GAAGAC 1 cut(s) 2276
BstX2I RGATCY 3 cut(s) 130, 1321, 1701
BstXI CCANNNNNNTGG 1 cut(s) 1897
BstYI RGATCY 3 cut(s) 130, 1321, 1701
Bsu15I ATCGAT 1 cut(s) 730
BsuRI GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
BsuTUI ATCGAT 1 cut(s) 730
BtgI CCRYGG 2 cut(s) 750, 1929
BtgZI GCGATG 1 cut(s) 2507
BtsCI GGATG 9 cut(s) 302, 493, 715, 956, 1939, 1945, 2260, 2431, 2449
BtsI GCAGTG 2 cut(s) 1078, 1408
BtsIMutI CAGTG 4 cut(s) 277, 1078, 1408, 2417
BveI ACCTGC 1 cut(s) 919
CaiI CAGNNNCTG 1 cut(s) 2204
CciI TCATGA 1 cut(s) 397
CfoI GCGC 2 cut(s) 261, 1690
Cfr10I RCCGGY 1 cut(s) 2622
Cfr13I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
ClaI ATCGAT 1 cut(s) 730
CseI GACGC 2 cut(s) 86, 1679
CsiI ACCWGGT 1 cut(s) 2344
Csp6I GTAC 4 cut(s) 1029, 1235, 2105, 2163
CviQI GTAC 4 cut(s) 1029, 1235, 2105, 2163
DdeI CTNAG 8 cut(s) 81, 127, 453, 1115, 1297, 1560, 1980, 2694
DpnI GATC 9 cut(s) 8, 132, 336, 416, 955, 1323, 1703, 1746, 2649
DpnII GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
DraI TTTAAA 3 cut(s) 364, 1966, 2041
DrdI GACNNNNNNGTC 1 cut(s) 2334
DriI GACNNNNNGTC 1 cut(s) 2714
DseDI GACNNNNNNGTC 1 cut(s) 2334
EaeI YGGCCR 2 cut(s) 747, 1669
Eam1104I CTCTTC 2 cut(s) 1101, 1979
Eam1105I GACNNNNNGTC 1 cut(s) 2714
EarI CTCTTC 2 cut(s) 1101, 1979
EciI GGCGGA 1 cut(s) 2328
Eco130I CCWWGG 3 cut(s) 307, 1929, 2332
Eco147I AGGCCT 2 cut(s) 939, 1990
Eco24I GRGCYC 1 cut(s) 2588
Eco31I GGTCTC 1 cut(s) 1171
Eco32I GATATC 1 cut(s) 525
Eco47I GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
Eco57I CTGAAG 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 1642
Eco91I GGTNACC 1 cut(s) 554
EcoNI CCTNNNNNAGG 1 cut(s) 944
EcoO109I RGGNCCY 2 cut(s) 320, 2584
EcoO65I GGTNACC 1 cut(s) 554
EcoRI GAATTC 1 cut(s) 2630
EcoRII CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
EcoRV GATATC 1 cut(s) 525
EcoT14I CCWWGG 3 cut(s) 307, 1929, 2332
EcoT22I ATGCAT 3 cut(s) 193, 1264, 2482
EcoT38I GRGCYC 1 cut(s) 2588
ErhI CCWWGG 3 cut(s) 307, 1929, 2332
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FaqI GGGAC 2 cut(s) 333, 605
FauI CCCGC 2 cut(s) 2296, 2371
FauNDI CATATG 2 cut(s) 1222, 2637
FbaI TGATCA 2 cut(s) 6, 1744
FblI GTMKAC 1 cut(s) 2317
FokI GGATG 9 cut(s) 289, 500, 722, 943, 1946, 1952, 2267, 2438, 2456
FriOI GRGCYC 1 cut(s) 2588
FspBI CTAG 2 cut(s) 1032, 2166
FspI TGCGCA 1 cut(s) 260
GlaI GCGC 2 cut(s) 260, 1689
GsaI CCCAGC 2 cut(s) 1319, 2026
GsuI CTGGAG 3 cut(s) 1058, 1119, 2181
HaeIII GGCC 7 cut(s) 331, 749, 939, 1671, 1990, 2586, 2727
HapII CCGG 1 cut(s) 2623
HgaI GACGC 2 cut(s) 86, 1679
HhaI GCGC 2 cut(s) 261, 1690
Hin1I GRCGYC 2 cut(s) 97, 2273
Hin6I GCGC 2 cut(s) 259, 1688
HinP1I GCGC 2 cut(s) 259, 1688
HincII GTYRAC 2 cut(s) 1926, 2318
HindII GTYRAC 2 cut(s) 1926, 2318
HindIII AAGCTT 1 cut(s) 981
HpaII CCGG 1 cut(s) 2623
HphI GGTGA 6 cut(s) 566, 730, 1328, 1826, 2420, 2651
Hpy166II GTNNAC 5 cut(s) 1926, 2318, 2377, 2560, 2579
Hpy8I GTNNAC 5 cut(s) 1926, 2318, 2377, 2560, 2579
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 5 cut(s) 310, 1205, 1544, 1669, 1812
HpyCH4III ACNGT 7 cut(s) 579, 724, 828, 1186, 1422, 2557, 2712
HpyCH4IV ACGT 4 cut(s) 61, 154, 1027, 2273
HpyF3I CTNAG 8 cut(s) 81, 127, 453, 1115, 1297, 1560, 1980, 2694
HpySE526I ACGT 4 cut(s) 61, 154, 1027, 2273
Hsp92I GRCGYC 2 cut(s) 97, 2273
HspAI GCGC 2 cut(s) 259, 1688
KroI GCCGGC 1 cut(s) 2622
KroNI GCCGGC 1 cut(s) 2624
Ksp22I TGATCA 2 cut(s) 6, 1744
Kzo9I GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
LmnI GCTCC 4 cut(s) 49, 1100, 1111, 2200
LweI GCATC 5 cut(s) 653, 1120, 1295, 1396, 2243
MabI ACCWGGT 1 cut(s) 2344
MaeI CTAG 2 cut(s) 1032, 2166
MaeII ACGT 4 cut(s) 61, 154, 1027, 2273
MaeIII GTNAC 7 cut(s) 231, 554, 626, 718, 815, 1334, 1341
MalI GATC 9 cut(s) 8, 132, 336, 416, 955, 1323, 1703, 1746, 2649
MboI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
MflI RGATCY 3 cut(s) 130, 1321, 1701
MhlI GDGCHC 2 cut(s) 58, 2588
MlsI TGGCCA 1 cut(s) 1671
MluNI TGGCCA 1 cut(s) 1671
MlyI GAGTC 4 cut(s) 587, 1126, 2390, 2408
MmeI TCCRAC 5 cut(s) 66, 80, 1349, 2167, 2421
Mox20I TGGCCA 1 cut(s) 1671
Mph1103I ATGCAT 3 cut(s) 193, 1264, 2482
MroNI GCCGGC 1 cut(s) 2622
MscI TGGCCA 1 cut(s) 1671
MslI CAYNNNNRTG 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 1671
MspA1I CMGCKG 5 cut(s) 537, 1397, 2291, 2436, 2674
MspCI CTTAAG 1 cut(s) 2545
MspI CCGG 1 cut(s) 2623
MspR9I CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
Mva1269I GAATGC 5 cut(s) 1014, 1350, 1453, 1651, 2480
MvaI CCWGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
MvnI CGCG 2 cut(s) 1690, 2526
NaeI GCCGGC 1 cut(s) 2624
NcoI CCATGG 1 cut(s) 1929
NdeI CATATG 2 cut(s) 1222, 2637
NdeII GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
NgoMIV GCCGGC 1 cut(s) 2622
NlaIV GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 3 cut(s) 554, 718, 1334
NsbI TGCGCA 1 cut(s) 260
NsiI ATGCAT 3 cut(s) 193, 1264, 2482
NspI RCATGY 2 cut(s) 1295, 1380
OliI CACNNNNGTG 1 cut(s) 270
PaeR7I CTCGAG 1 cut(s) 1642
PagI TCATGA 1 cut(s) 397
PceI AGGCCT 2 cut(s) 939, 1990
PcsI WCGNNNNNNNCGW 3 cut(s) 334, 736, 1000
PctI GAATGC 5 cut(s) 1014, 1350, 1453, 1651, 2480
PdiI GCCGGC 1 cut(s) 2624
PfeI GAWTC 6 cut(s) 458, 805, 1564, 1602, 1766, 2413
PflMI CCANNNNNTGG 2 cut(s) 662, 2029
PleI GAGTC 4 cut(s) 587, 1125, 2389, 2407
PpsI GAGTC 4 cut(s) 587, 1125, 2389, 2407
Ppu21I YACGTR 2 cut(s) 155, 1028
PpuMI RGGWCCY 1 cut(s) 320
PsiI TTATAA 1 cut(s) 2391
Psp1406I AACGTT 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 320
Psp6I CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
PspEI GGTNACC 1 cut(s) 554
PspFI CCCAGC 2 cut(s) 1315, 2022
PspGI CCWGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
PspN4I GGNNCC 9 cut(s) 321, 668, 1323, 1703, 2034, 2136, 2196, 2457, 2586
PspOMI GGGCCC 1 cut(s) 2584
PspPI GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
PspPPI RGGWCCY 1 cut(s) 320
PstI CTGCAG 1 cut(s) 2679
PstNI CAGNNNCTG 1 cut(s) 2204
PsuI RGATCY 3 cut(s) 130, 1321, 1701
PvuII CAGCTG 3 cut(s) 1397, 2436, 2674
RsaI GTAC 4 cut(s) 1030, 1236, 2106, 2164
RsaNI GTAC 4 cut(s) 1029, 1235, 2105, 2163
RseI CAYNNNNRTG 1 cut(s) 270
SalI GTCGAC 1 cut(s) 2316
Sau3AI GATC 9 cut(s) 6, 130, 334, 414, 953, 1321, 1701, 1744, 2647
Sau96I GGNCC 7 cut(s) 184, 320, 1904, 2243, 2336, 2584, 2585
ScaI AGTACT 2 cut(s) 2106, 2164
SchI GAGTC 4 cut(s) 587, 1126, 2390, 2408
ScrFI CCNGG 7 cut(s) 142, 746, 1037, 1347, 1706, 2346, 2574
SduI GDGCHC 2 cut(s) 58, 2588
SexAI ACCWGGT 1 cut(s) 2344
SfaNI GCATC 5 cut(s) 653, 1120, 1295, 1396, 2243
SfcI CTRYAG 2 cut(s) 288, 2675
Sfr274I CTCGAG 1 cut(s) 1642
SinI GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
SlaI CTCGAG 1 cut(s) 1642
SmiI ATTTAAAT 1 cut(s) 2041
SmiMI CAYNNNNRTG 1 cut(s) 270
SmlI CTYRAG 4 cut(s) 179, 622, 1642, 2545
SmoI CTYRAG 4 cut(s) 179, 622, 1642, 2545
SpeI ACTAGT 1 cut(s) 2165
SseBI AGGCCT 2 cut(s) 939, 1990
SsiI CCGC 8 cut(s) 244, 535, 1709, 2252, 2289, 2339, 2364, 2728
SspI AATATT 1 cut(s) 1632
SspMI CTAG 2 cut(s) 1032, 2166
StuI AGGCCT 2 cut(s) 939, 1990
StyD4I CCNGG 7 cut(s) 140, 744, 1035, 1345, 1704, 2344, 2572
StyI CCWWGG 3 cut(s) 307, 1929, 2332
SwaI ATTTAAAT 1 cut(s) 2041
TaaI ACNGT 7 cut(s) 579, 724, 828, 1186, 1422, 2557, 2712
TaiI ACGT 4 cut(s) 64, 157, 1030, 2276
TaqI TCGA 7 cut(s) 69, 469, 730, 1159, 1643, 2317, 2643
TatI WGTACW 3 cut(s) 1234, 2104, 2162
TauI GCSGC 2 cut(s) 1712, 2730
TfiI GAWTC 6 cut(s) 458, 805, 1564, 1602, 1766, 2413
TscAI CASTG 4 cut(s) 277, 1078, 1408, 2424
TseFI GTSAC 3 cut(s) 554, 718, 1334
Tsp45I GTSAC 3 cut(s) 554, 718, 1334
TspDTI ATGAA 9 cut(s) 180, 524, 1178, 1428, 1560, 1594, 1823, 1965, 2679
TspGWI ACGGA 2 cut(s) 750, 992
TspRI CASTG 4 cut(s) 277, 1078, 1408, 2424
Van91I CCANNNNNTGG 2 cut(s) 662, 2029
Vha464I CTTAAG 1 cut(s) 2545
VpaK11BI GGWCC 5 cut(s) 184, 320, 1904, 2243, 2336
XagI CCTNNNNNAGG 1 cut(s) 944
XapI RAATTY 2 cut(s) 1781, 2630
XceI RCATGY 2 cut(s) 1295, 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 1861
XhoI CTCGAG 1 cut(s) 1642
XmiI GTMKAC 1 cut(s) 2317
XspI CTAG 2 cut(s) 1032, 2166
ZraI GACGTC 1 cut(s) 2274
ZrmI AGTACT 2 cut(s) 2106, 2164
Zsp2I ATGCAT 3 cut(s) 193, 1264, 2482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.