Rw0G013670

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00614
OG0000085
Physical Location & Seq
Forward (+)
1425 .. 4860
3436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G013670.1

Sequence Viewer

Length: 3336 bp
ATGGTTGAAACCCCAGAGCCAATATCAGAGGATAATCAAGAACTGGTTGACGTCGATGCCTCTGCATCTCTTCCTTCATCATCAGCTCCTAGGTGGAAGCATGACGTGTTTTTGAGTTTCAGGGGTGAGGAAACTCGTAGGGGTATTACATCCTTTTTATACGATCGACTGAAAAACAGAGGAATCAAAACATTCATGGATGACCAAGACCTTCAAGTAGGGGATGTTATTTCTCCCACTCTCCTAATGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCCAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAACGCATGAAAGAAGACAACAATAGAATTCTGCCACTTTTTTATAATGTGGATCCTAGTGATGTACGAAATCAGAAGAGGAGTTTTGGAGATGCTTTCACTAAGCATGAAAAATCTGGGAAACACAAATCAGGAAAGGTGCAGCAGTGGAGAGAAGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCATACACTGAATTATAAAACTGATAGAGAACTTGTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGACTGACTGAACCTGTGCTTAAAATGTGCATCGGAGGTTTTAAAGAATTTGCAGCAACAAAACAAGCCATTGATAAGGTCATGAACGCGCTAAAAGATGATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGAAAGACGACAATGGTGAAATATGTTGGAGCACAAGCCCAAAAAAGTAGGCTTTTTAATCAAGTGATAATGGCTGTCGTATCCCAAAACCCCGACTTGATGAAAATTCAACAGACATTTGCAGAAATGCTGGGCTTGAAATTGGAGGAGAAGACAGAAATTGTAAGAGCCAGTAAATTGAAGGAGAAGATATTGGGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGCATAGACTTTTCAAGCATTGGAATTCCAAGCCACAACGAACTTCAAAGATGCAATTCCAAAGTTCTACTGACTACCAGAAAATTGACTGTTTGCCATTCCATGGATTGCCATGCAAAAGTACATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAAGGAAGTACCCTCTTGACAAATCATCCACTTTCTATGATGTGGCACGTAAGGTGGCTAGAGAATGCGCCGGTCTACCACTTGCTTTGATAGCAGTCGCGAGGGCCCTTCGAGGTCAAGGTTTGGATGGATGGAAAGAAGCTGCTCGACAACTACAAGCGTCTCAACCTCCCAACCCTGAAGATGAGGGAGTTGTGTATGCATCCATAAAGTTAAGCTATGATTACTTGAAATCTGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGCTCATGTATGGAATTGGGAAAGGAATGTTTCAAGATTCCAACATGCTAGAAGCCCGAGACAGAACATATTTAGTGGTGAAGGCCCTTAAAGATTCTAGCTTGCTTTTGGATGCTAGAGATGACGGATGTGTAAGGATGCATGATGTCATTCGGGACGTGGCAATATCAATTTCATTATCTGAAGACGGCCAGCGGTTCTTGGTGAAAAGTAGCTATGAATTGAAGAACTGGCCTAAGATTGATGCACATAAAGGCTACTCTGCAATCTCACTAATGAAGAACGAAATTTGCAAGCTACCGGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTACAACACAATGCTTCTTTCAATGAGATCCCAAAATCATTTTTCCAGAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCTACACTCGTATTTCATTACTACCCCAATCAATCAGTTCTCTAACCAACCTTCAAGCTTTGTATTTAGATTATTGCTGGAACATAAATGACATTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGCATTTGCTCTGGAAGAATTGTCAAGAGAAATAGGACAGTTGACCAATCTAAGGATGCTGGATGTCAGTGCTGTATATATTCGCACAATCCCATCTAAAGTGATATCCAAGTTGCATCAGTTGGAAGAACTGTACATGGAATGTGGATTTTTGAACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGGTTTATTATATTTAAGCATTTTGACAGTTTGCATATCGGATGCAAATGGCATCCCTAAAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTCGATATCAGTATTCGCAGCGGCCATAGTGGAGGGAGATTCATTAAAAAACTTCGATCCGGTTGTAATTGTAGATCCTTGAGCCTTTATAGAACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAATGCTGTGGTAAAGAATACTGAGAGGCTGCGTTATAAAGAATGCAAAGGGTTACGTAACATTCTTGTGGAATATGACCGTGGGAGGTTTCATGGACTGAAAGTTCTCTCTGTAATTGGTCAGTGTGCGAACTTGAAAGAACTGATGAATGCAATAACATGTGTTCCACATATGCCTGTGTTTGAGAACTTGGAAGAGTTGTATCTGGAAGACCTGTGTCATCTGAGGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCTGTATGTGTCCTATTTTTGGGAATGCACTGTTGCCATCAAAACTGTTGCAGAAACTAGCAAATCTGGAAAAACTAAACTGTGAGACAAGTAAAGTGAAACATGTTTTTGGATGTGAAGGATTTGAGCTAGATCATACAAATCTGAGAGAGATGGTGTTGTTGGATCTACCCGCAGTAAGAAGCATGTGTAATGGTCCTGCTCCACGTGGAATGTTCCGGACTCTAAAGAAATTGTTCATTATGAAATGCAACTTCCGGGGAAGTCTCTTCACATTTGATGCAGCTCAGTGTCTTTTCCAATTGGAAAACCTTGATGTAACCATCTGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAACAACAAGAAGACCATTCTTCCAAAATTGAAGTACTTATGTTTGGAATATCTTCCAATGTTGTACAAGGGAAATGCTACTATTGATTTTGAGTGTCCTTCATTGGAATACTTGCGTATGCAGGAATGCCCCCACTTGTCATTTCCATCCTCTGTTTCTGACTACTTCAACAGCAGGAATCAAGTTCTTTTCAATGATGGTCGTTTTCTGAATCGGATGCGCAGATAG

Protein Analysis

1111

Amino Acids

126.13

Weight (kDa)

7.59

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 33 - 203 6.2e-52 TIR domain
TIR_2 PF13676 36 - 144 6e-17 TIR domain
NB-ARC PF00931 220 - 385 1.8e-32 NB-ARC domain
LRR_8 PF13855 592 - 650 5.3e-10 Leucine rich repeat
LRR_14 PF23598 647 - 718 7.2e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 881 - 977 7.6e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 966 - 1089 6.5e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 384, 543, 2487
AasI GACNNNNNNGTC 1 cut(s) 2447
AatII GACGTC 1 cut(s) 54
Acc16I TGCGCA 1 cut(s) 3329
AccB7I CCANNNNNTGG 1 cut(s) 1078
AccI GTMKAC 2 cut(s) 720, 1225
AccII CGCG 2 cut(s) 686, 1250
AccIII TCCGGA 2 cut(s) 2437, 2955
AciI CCGC 3 cut(s) 1651, 2340, 2910
AcoI YGGCCR 2 cut(s) 1645, 2341
AcsI RAATTY 6 cut(s) 366, 514, 644, 843, 999, 1743
AcuI CTGAAG 2 cut(s) 1350, 1659
AcvI CACGTG 1 cut(s) 2945
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 312
AfaI GTAC 6 cut(s) 405, 1098, 1160, 2147, 3143, 3173
AfiI CCNNNNNNNGG 4 cut(s) 1078, 2064, 2756, 3073
AflIII ACRYGT 3 cut(s) 105, 2609, 2839
AjiI CACGTC 2 cut(s) 106, 1615
AjnI CCWGG 1 cut(s) 2704
AjuI GAANNNNNNNTTGG 4 cut(s) 1388, 1420, 2193, 2225
AloI GAACNNNNNNTCC 2 cut(s) 2538, 2570
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 4 cut(s) 1317, 1509, 2816, 3008
Ama87I CYCGRG 1 cut(s) 1512
Aor13HI TCCGGA 2 cut(s) 2437, 2955
AoxI GGCC 6 cut(s) 702, 1254, 1539, 1645, 1688, 2341
ApaI GGGCCC 1 cut(s) 1258
ApeKI GCWGC 7 cut(s) 481, 650, 1292, 1416, 2337, 2479, 3020
ApoI RAATTY 6 cut(s) 366, 514, 644, 843, 999, 1743
Asp700I GAANNNNTTC 2 cut(s) 2972, 3159
AspA2I CCTAGG 1 cut(s) 89
AspLEI GCGC 3 cut(s) 688, 1220, 3330
AspS9I GGNCC 4 cut(s) 1254, 1255, 1540, 2933
AsuC2I CCSGG 2 cut(s) 943, 2996
AsuHPI GGTGA 5 cut(s) 137, 766, 1546, 1672, 2705
AvaI CYCGRG 1 cut(s) 1512
AvaII GGWCC 1 cut(s) 2933
AvrII CCTAGG 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 1258
BamHI GGATCC 2 cut(s) 391, 945
BanII GRGCYC 1 cut(s) 1258
BbrPI CACGTG 1 cut(s) 2945
BbsI GAAGAC 5 cut(s) 360, 896, 1647, 2667, 3125
Bbv12I GWGCWC 1 cut(s) 772
BbvI GCAGC 7 cut(s) 493, 662, 1279, 1403, 2349, 2466, 3032
BceAI ACGGC 1 cut(s) 1660
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BciT130I CCWGG 1 cut(s) 2706
BciVI GTATCC 1 cut(s) 829
BcnI CCSGG 2 cut(s) 943, 2996
BcoDI GTCTC 4 cut(s) 1317, 1509, 2816, 3008
BfaI CTAG 8 cut(s) 90, 396, 1209, 1505, 1554, 1572, 2795, 2867
BfmI CTRYAG 1 cut(s) 2716
BfuI GTATCC 1 cut(s) 829
BisI GCNGC 8 cut(s) 482, 651, 1293, 1417, 2338, 2341, 2480, 3021
BlnI CCTAGG 1 cut(s) 89
BlsI GCNGC 8 cut(s) 483, 652, 1294, 1418, 2339, 2342, 2481, 3022
BmcAI AGTACT 1 cut(s) 3143
Bme1390I CCNGG 3 cut(s) 943, 2706, 2996
Bme18I GGWCC 1 cut(s) 2933
BmeT110I CYCGRG 1 cut(s) 1512
BmgBI CACGTC 2 cut(s) 106, 1615
BmgT120I GGNCC 4 cut(s) 1254, 1255, 1540, 2933
BmiI GGNNCC 5 cut(s) 393, 940, 947, 1256, 2431
BmrFI CCNGG 3 cut(s) 943, 2706, 2996
BmrI ACTGGG 1 cut(s) 2179
BmuI ACTGGG 1 cut(s) 2179
BoxI GACNNNNGTC 1 cut(s) 2667
BpiI GAAGAC 5 cut(s) 360, 896, 1647, 2667, 3125
BpmI CTGGAG 1 cut(s) 1766
BpuEI CTTGAG 2 cut(s) 2015, 2419
BpuMI CCSGG 2 cut(s) 943, 2996
BsaAI YACGTR 3 cut(s) 1199, 2507, 2945
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 4 cut(s) 89, 1077, 2530, 2995
BsaWI WCCGGW 4 cut(s) 1756, 2378, 2437, 2955
Bsc4I CCNNNNNNNGG 4 cut(s) 1078, 2064, 2756, 3073
Bse118I RCCGGY 1 cut(s) 1220
Bse1I ACTGG 5 cut(s) 48, 909, 1691, 2174, 2447
BseAI TCCGGA 2 cut(s) 2437, 2955
BseBI CCWGG 1 cut(s) 2706
BseDI CCNNGG 4 cut(s) 89, 1077, 2530, 2995
BseLI CCNNNNNNNGG 4 cut(s) 1078, 2064, 2756, 3073
BseMII CTCAG 4 cut(s) 2463, 2666, 2873, 3038
BseNI ACTGG 5 cut(s) 48, 909, 1691, 2174, 2447
BseRI GAGGAG 2 cut(s) 433, 899
BseSI GKGCMC 1 cut(s) 1258
BseXI GCAGC 7 cut(s) 493, 662, 1279, 1403, 2349, 2466, 3032
BseYI CCCAGC 1 cut(s) 868
BsgI GTGCAG 1 cut(s) 500
Bsh1236I CGCG 2 cut(s) 686, 1250
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 6 cut(s) 704, 1256, 1541, 1647, 1690, 2343
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 772
BsiHKCI CYCGRG 1 cut(s) 1512
BsiSI CCGG 7 cut(s) 942, 1221, 1757, 2379, 2438, 2956, 2995
BslFI GGGAC 1 cut(s) 1625
BslI CCNNNNNNNGG 4 cut(s) 1078, 2064, 2756, 3073
BsmAI GTCTC 4 cut(s) 1317, 1509, 2816, 3008
BsmBI CGTCTC 1 cut(s) 1317
BsmFI GGGAC 1 cut(s) 1625
BsmI GAATGC 5 cut(s) 1220, 2498, 2605, 2767, 3239
BsnI GGCC 6 cut(s) 704, 1256, 1541, 1647, 1690, 2343
BsoBI CYCGRG 1 cut(s) 1512
Bsp120I GGGCCC 1 cut(s) 1254
Bsp1286I GDGCHC 2 cut(s) 772, 1258
Bsp13I TCCGGA 2 cut(s) 2437, 2955
Bsp1407I TGTACA 2 cut(s) 2145, 3171
Bsp19I CCATGG 1 cut(s) 1077
Bsp68I TCGCGA 1 cut(s) 1250
BspACI CCGC 3 cut(s) 1651, 2340, 2910
BspANI GGCC 6 cut(s) 704, 1256, 1541, 1647, 1690, 2343
BspCNI CTCAG 4 cut(s) 2464, 2667, 2874, 3037
BspEI TCCGGA 2 cut(s) 2437, 2955
BspFNI CGCG 2 cut(s) 686, 1250
BspHI TCATGA 1 cut(s) 678
BspLI GGNNCC 5 cut(s) 393, 940, 947, 1256, 2431
BspMAI CTGCAG 1 cut(s) 2720
BspQI GCTCTTC 1 cut(s) 968
BsrFI RCCGGY 1 cut(s) 1220
BsrGI TGTACA 2 cut(s) 2145, 3171
BsrI ACTGG 5 cut(s) 48, 909, 1691, 2174, 2447
BssAI RCCGGY 1 cut(s) 1220
BssECI CCNNGG 4 cut(s) 89, 1077, 2530, 2995
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 89, 1077
Bst2UI CCWGG 1 cut(s) 2706
Bst4CI ACNGT 8 cut(s) 1066, 2052, 2145, 2257, 2531, 2769, 2784, 2819
Bst6I CTCTTC 7 cut(s) 75, 307, 410, 968, 1755, 2640, 3011
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 2 cut(s) 2145, 3171
BstBAI YACGTR 3 cut(s) 1199, 2507, 2945
BstC8I GCNNGC 3 cut(s) 1559, 1649, 1751
BstDSI CCRYGG 2 cut(s) 1077, 2530
BstFNI CGCG 2 cut(s) 686, 1250
BstHHI GCGC 3 cut(s) 688, 1220, 3330
BstMAI GTCTC 4 cut(s) 1317, 1509, 2816, 3008
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 1241
BstNI CCWGG 1 cut(s) 2706
BstNSI RCATGY 4 cut(s) 1504, 2613, 2843, 2926
BstPAI GACNNNNGTC 1 cut(s) 2667
BstSCI CCNGG 3 cut(s) 941, 2704, 2994
BstSFI CTRYAG 1 cut(s) 2716
BstSLI GKGCMC 1 cut(s) 1258
BstSNI TACGTA 1 cut(s) 2507
BstUI CGCG 2 cut(s) 686, 1250
BstV1I GCAGC 7 cut(s) 493, 662, 1279, 1403, 2349, 2466, 3032
BstV2I GAAGAC 5 cut(s) 360, 896, 1647, 2667, 3125
BstX2I RGATCY 7 cut(s) 391, 945, 1821, 1867, 2393, 2708, 2902
BstYI RGATCY 7 cut(s) 391, 945, 1821, 1867, 2393, 2708, 2902
BsuI GTATCC 1 cut(s) 829
BsuRI GGCC 6 cut(s) 704, 1256, 1541, 1647, 1690, 2343
BtgI CCRYGG 2 cut(s) 1077, 2530
BtrI CACGTC 2 cut(s) 106, 1615
BtsI GCAGTG 1 cut(s) 491
BtsIMutI CAGTG 6 cut(s) 491, 533, 2086, 2579, 2765, 3032
BtuMI TCGCGA 1 cut(s) 1250
Cac8I GCNNGC 3 cut(s) 1559, 1649, 1751
CciI TCATGA 1 cut(s) 678
CfoI GCGC 3 cut(s) 688, 1220, 3330
Cfr10I RCCGGY 1 cut(s) 1220
Cfr13I GGNCC 4 cut(s) 1254, 1255, 1540, 2933
CseI GACGC 1 cut(s) 1299
Csp6I GTAC 6 cut(s) 404, 1097, 1159, 2146, 3142, 3172
CspCI CAANNNNNGTGG 4 cut(s) 694, 729, 1218, 1253
CviQI GTAC 6 cut(s) 404, 1097, 1159, 2146, 3142, 3172
DraI TTTAAA 2 cut(s) 501, 640
DraIII CACNNNGTG 1 cut(s) 312
DrdI GACNNNNNNGTC 1 cut(s) 2447
DseDI GACNNNNNNGTC 1 cut(s) 2447
EaeI YGGCCR 2 cut(s) 1645, 2341
Eam1104I CTCTTC 7 cut(s) 75, 307, 410, 968, 1755, 2640, 3011
EarI CTCTTC 7 cut(s) 75, 307, 410, 968, 1755, 2640, 3011
Eco105I TACGTA 1 cut(s) 2507
Eco130I CCWWGG 2 cut(s) 89, 1077
Eco24I GRGCYC 1 cut(s) 1258
Eco32I GATATC 3 cut(s) 1441, 2118, 2326
Eco47I GGWCC 1 cut(s) 2933
Eco57I CTGAAG 2 cut(s) 1350, 1659
Eco72I CACGTG 1 cut(s) 2945
Eco88I CYCGRG 1 cut(s) 1512
EcoO109I RGGNCCY 3 cut(s) 1254, 1255, 1540
EcoRI GAATTC 2 cut(s) 366, 999
EcoRII CCWGG 1 cut(s) 2704
EcoRV GATATC 3 cut(s) 1441, 2118, 2326
EcoT14I CCWWGG 2 cut(s) 89, 1077
EcoT22I ATGCAT 2 cut(s) 1354, 1599
EcoT38I GRGCYC 1 cut(s) 1258
ErhI CCWWGG 2 cut(s) 89, 1077
Esp3I CGTCTC 1 cut(s) 1317
FaqI GGGAC 1 cut(s) 1625
FauI CCCGC 1 cut(s) 2917
FauNDI CATATG 1 cut(s) 2622
FblI GTMKAC 2 cut(s) 720, 1225
Fnu4HI GCNGC 8 cut(s) 482, 651, 1293, 1417, 2338, 2341, 2480, 3021
FriOI GRGCYC 1 cut(s) 1258
Fsp4HI GCNGC 8 cut(s) 482, 651, 1293, 1417, 2338, 2341, 2480, 3021
FspBI CTAG 8 cut(s) 90, 396, 1209, 1505, 1554, 1572, 2795, 2867
FspI TGCGCA 1 cut(s) 3329
GlaI GCGC 3 cut(s) 687, 1219, 3329
GluI GCNGC 8 cut(s) 482, 651, 1293, 1417, 2338, 2341, 2480, 3021
GsaI CCCAGC 1 cut(s) 872
GsuI CTGGAG 1 cut(s) 1766
HaeIII GGCC 6 cut(s) 704, 1256, 1541, 1647, 1690, 2343
HapII CCGG 7 cut(s) 942, 1221, 1757, 2379, 2438, 2956, 2995
HgaI GACGC 1 cut(s) 1299
HhaI GCGC 3 cut(s) 688, 1220, 3330
Hin1I GRCGYC 1 cut(s) 51
Hin6I GCGC 3 cut(s) 686, 1218, 3328
HinP1I GCGC 3 cut(s) 686, 1218, 3328
HincII GTYRAC 2 cut(s) 49, 2055
HindII GTYRAC 2 cut(s) 49, 2055
HindIII AAGCTT 3 cut(s) 495, 1929, 1991
HpaII CCGG 7 cut(s) 942, 1221, 1757, 2379, 2438, 2956, 2995
HphI GGTGA 5 cut(s) 137, 766, 1546, 1672, 2705
Hpy166II GTNNAC 6 cut(s) 49, 341, 721, 1226, 2055, 2317
Hpy8I GTNNAC 6 cut(s) 49, 341, 721, 1226, 2055, 2317
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 8 cut(s) 1066, 2052, 2145, 2257, 2531, 2769, 2784, 2819
HpyCH4IV ACGT 6 cut(s) 51, 105, 1198, 1614, 2506, 2944
HpyF10VI GCNNNNNNNGC 1 cut(s) 1241
HpySE526I ACGT 6 cut(s) 51, 105, 1198, 1614, 2506, 2944
Hsp92I GRCGYC 1 cut(s) 51
HspAI GCGC 3 cut(s) 686, 1218, 3328
Kpn2I TCCGGA 2 cut(s) 2437, 2955
LguI GCTCTTC 1 cut(s) 968
LmnI GCTCC 4 cut(s) 91, 767, 1131, 2944
Lsp1109I GCAGC 7 cut(s) 493, 662, 1279, 1403, 2349, 2466, 3032
MaeI CTAG 8 cut(s) 90, 396, 1209, 1505, 1554, 1572, 2795, 2867
MaeII ACGT 6 cut(s) 51, 105, 1198, 1614, 2506, 2944
MaeIII GTNAC 4 cut(s) 2502, 2507, 2697, 3055
MfeI CAATTG 2 cut(s) 1446, 3038
MflI RGATCY 7 cut(s) 391, 945, 1821, 1867, 2393, 2708, 2902
MhlI GDGCHC 2 cut(s) 772, 1258
MlyI GAGTC 4 cut(s) 726, 1852, 1869, 2953
MmeI TCCRAC 5 cut(s) 718, 745, 1521, 2115, 2880
Mph1103I ATGCAT 2 cut(s) 1354, 1599
MroI TCCGGA 2 cut(s) 2437, 2955
MroXI GAANNNNTTC 2 cut(s) 2972, 3159
MslI CAYNNNNRTG 1 cut(s) 2516
MspA1I CMGCKG 2 cut(s) 1651, 2340
MspI CCGG 7 cut(s) 942, 1221, 1757, 2379, 2438, 2956, 2995
MspR9I CCNGG 3 cut(s) 943, 2706, 2996
MunI CAATTG 2 cut(s) 1446, 3038
Mva1269I GAATGC 5 cut(s) 1220, 2498, 2605, 2767, 3239
MvaI CCWGG 1 cut(s) 2706
MvnI CGCG 2 cut(s) 686, 1250
MwoI GCNNNNNNNGC 1 cut(s) 1241
NciI CCSGG 2 cut(s) 943, 2996
NcoI CCATGG 1 cut(s) 1077
NdeI CATATG 1 cut(s) 2622
NlaIV GGNNCC 5 cut(s) 393, 940, 947, 1256, 2431
NruI TCGCGA 1 cut(s) 1250
NsbI TGCGCA 1 cut(s) 3329
NsiI ATGCAT 2 cut(s) 1354, 1599
NspI RCATGY 4 cut(s) 1504, 2613, 2843, 2926
PagI TCATGA 1 cut(s) 678
PciI ACATGT 2 cut(s) 2609, 2839
PciSI GCTCTTC 1 cut(s) 968
PctI GAATGC 5 cut(s) 1220, 2498, 2605, 2767, 3239
PdmI GAANNNNTTC 2 cut(s) 2972, 3159
PflMI CCANNNNNTGG 1 cut(s) 1078
PkrI GCNGC 8 cut(s) 483, 652, 1294, 1418, 2339, 2342, 2481, 3022
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 4 cut(s) 725, 1851, 1868, 2953
PmaCI CACGTG 1 cut(s) 2945
PmlI CACGTG 1 cut(s) 2945
PpsI GAGTC 4 cut(s) 725, 1851, 1868, 2953
Ppu21I YACGTR 3 cut(s) 1199, 2507, 2945
PscI ACATGT 2 cut(s) 2609, 2839
PshAI GACNNNNGTC 1 cut(s) 2667
PsiI TTATAA 3 cut(s) 384, 543, 2487
Psp6I CCWGG 1 cut(s) 2704
PspCI CACGTG 1 cut(s) 2945
PspFI CCCAGC 1 cut(s) 868
PspGI CCWGG 1 cut(s) 2704
PspN4I GGNNCC 5 cut(s) 393, 940, 947, 1256, 2431
PspOMI GGGCCC 1 cut(s) 1254
PspPI GGNCC 4 cut(s) 1254, 1255, 1540, 2933
PstI CTGCAG 1 cut(s) 2720
PsuI RGATCY 7 cut(s) 391, 945, 1821, 1867, 2393, 2708, 2902
PvuI CGATCG 1 cut(s) 166
RruI TCGCGA 1 cut(s) 1250
RsaI GTAC 6 cut(s) 405, 1098, 1160, 2147, 3143, 3173
RsaNI GTAC 6 cut(s) 404, 1097, 1159, 2146, 3142, 3172
RseI CAYNNNNRTG 1 cut(s) 2516
SapI GCTCTTC 1 cut(s) 968
SatI GCNGC 8 cut(s) 482, 651, 1293, 1417, 2338, 2341, 2480, 3021
Sau96I GGNCC 4 cut(s) 1254, 1255, 1540, 2933
ScaI AGTACT 1 cut(s) 3143
SchI GAGTC 4 cut(s) 726, 1852, 1869, 2953
ScrFI CCNGG 3 cut(s) 943, 2706, 2996
SduI GDGCHC 2 cut(s) 772, 1258
SfcI CTRYAG 1 cut(s) 2716
SinI GGWCC 1 cut(s) 2933
SmiMI CAYNNNNRTG 1 cut(s) 2516
SmlI CTYRAG 2 cut(s) 1994, 2398
SmoI CTYRAG 2 cut(s) 1994, 2398
SnaBI TACGTA 1 cut(s) 2507
SsiI CCGC 3 cut(s) 1651, 2340, 2910
SspI AATATT 1 cut(s) 2209
SspMI CTAG 8 cut(s) 90, 396, 1209, 1505, 1554, 1572, 2795, 2867
StyD4I CCNGG 3 cut(s) 941, 2704, 2994
StyI CCWWGG 2 cut(s) 89, 1077
TaaI ACNGT 8 cut(s) 1066, 2052, 2145, 2257, 2531, 2769, 2784, 2819
TaiI ACGT 6 cut(s) 54, 108, 1201, 1617, 2509, 2947
TaqI TCGA 8 cut(s) 54, 166, 1261, 1297, 2301, 2322, 2374, 3087
TatI WGTACW 4 cut(s) 1096, 2145, 3141, 3171
TauI GCSGC 1 cut(s) 2343
TscAI CASTG 6 cut(s) 491, 540, 2086, 2579, 2772, 3032
TseI GCWGC 7 cut(s) 481, 650, 1292, 1416, 2337, 2479, 3020
TspGWI ACGGA 2 cut(s) 738, 1596
TspRI CASTG 6 cut(s) 491, 540, 2086, 2579, 2772, 3032
Van91I CCANNNNNTGG 1 cut(s) 1078
VpaK11BI GGWCC 1 cut(s) 2933
XapI RAATTY 6 cut(s) 366, 514, 644, 843, 999, 1743
XceI RCATGY 4 cut(s) 1504, 2613, 2843, 2926
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 313
XmaJI CCTAGG 1 cut(s) 89
XmiI GTMKAC 2 cut(s) 720, 1225
XmnI GAANNNNTTC 2 cut(s) 2972, 3159
XspI CTAG 8 cut(s) 90, 396, 1209, 1505, 1554, 1572, 2795, 2867
ZraI GACGTC 1 cut(s) 52
ZrmI AGTACT 1 cut(s) 3143
Zsp2I ATGCAT 2 cut(s) 1354, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.