Rw5G042590

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Reverse (-)
74562503 .. 74565632
3130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042590.1

Sequence Viewer

Length: 2739 bp
ATGGCCCAAGCAGCCTCTGCATCGGCTGAATCTTCATCAGCTCGTCGGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTGAAGACGTTCGCAAGACTTTTCTCTCCCATTTATACCATGAATTGCAAAAGACGAGAAGCATTTTTGCATATAAGGATGATGAAGAGCTTGAAGTAGGGGCTCCTATTTCTCCAAGTCTCTTAAAGGCAATCGAAGAATCAAGGTTGGCCATCATTGTTCTATCGCCAAACTATGCTTCTTCTCCATGGTGTTTGGAGGAACTTACGAAGATTGTTCATTGCATGGAAGACAACAACAGAATTCTGCCACTTTTTTATCATGTGGAACCAGCTGATGTACGACATCAGAAGAACAGTTACGAGCTAGCATTCACCAGGCATGAAGAAAAGTCAAGGCATGGCATGGAGAAGCTGACGCAGTGGAGAGATGCTTTAAAGAAGGTGGCCAATTTCTCTGGGTGGCATACACAGAGTTATAAGACGGAAAGAGAACTCGTCGAAGTCATTGTGGACTCTGTGTGCAGTAAGGTACGACCAATGGAAATTGAGTTTTCAATGTCAACCGGAGATTTTGAAGAATTTGAAGCAACAAGACAAGCCATGTCTGAGGTGATGGATGCGCTAAAAGATGACACAGTCATTGCCGTTGCGGTGTATGGAATGGGAGGGGTGGGGAAGACAACTATGGTGGAACAGGTGGGTTTACGAGCTCAAAAAAGTGGGATTTTCCAACATGTGATTATGGTTGTCATATCCCAAACGCCCAACTTGAGAAATATTCAAGGCACATTGGCAGATCTGCTAGGCTTGGAATTGAAGGAGGAGACAAACGTTGGAAGAGCTACTAGATTGAAGAAGGAGATAATGAGAAGACACAAGATGCTTATAGTCTTGGACGACATTTGGGAGAGAATAGATTTATCAAGCATAGGAATTCCCAGCTACGACCAGCTTCAAAATTGCAATTCCAAAGTCCTACTCACCACAAGGAGATTGAATGTTTGTCATTCCATGGGGACCCAATCAAGCATTCCTCTGAATATCTTATCAGAGGAAGATTCTTGGAAGTTGTTTGAGAAGAAAGCAAGAAAACATCTTGAAAAATCAACTGAATTCTATGATGTAGCAAGGAAAGTGGCTAGCGAGTGTGCTGGTCTCCCGATCGCGTTGATAACAGTTGCGAGGGCAGTTGGAGATAAGGATCTTCAGGAATGGAAAGAAGCAGCTGAACGACTTGAGATGGCTCAACCTACTAACCTTGACGATAAGGGAGATGTGTACAAATGTATAAAGTTGAGCTATGACTACTTGCATGATACTGATGCCAAGTCATGCTTCTTGCTTTGCTGCTTATTCCCAGAAGATGATGATATCGCAATAGAAAGCTTGTTGAAGTATGGGTTAGGGAAAGGATTGTTTCGAGATGTCAACACAAACCAAACAATTCAAAAAGCTAGAGCCAAAATGTACATGGTGGTCAAGTATCTTGTAGCTGCTAACTTGCTTTTGAATGGTAGAGGGGATGGACACGTAAGGATGCATGATGTCATTCGAGATATGGCTATGTCAATTGCTTTGTCAGAAGATGGACATGGGTTTTTTGTAAGAACTCGTCGTGAATTAAGAGACTGGCCAATCAATGCAAATGATTGCTACTCAGCAATCTCTCTGATGGAGAACAACATTAGCAAGCTTCCCAAAGAGTTGGTATGTTCAAAATTGCAGATTTTATTGCTAAATAGAAATGCTGATTTGAAAGAGATCTCAGATTCCTTGTTTCAAAGTCCGAATGCATTAAGGGTCTTAGATATTAGTCAAACTCATATTTCATCATTACCCCCATCATTCGGTCTTCTGTCCAATCTTCAAGCTCTCTATGTAGATTATTGCAACTCAAGAATTGACATATCCATACTTACAAAACTGAAGAAACTTGAGATTCTTAGTATGAGAGGATGTGATATTAAGATATTTCCTATTAAAATAGGACATTTGACCTCTTTGAGAATTTTGGACGTCACTGGTGGACGTTTTGATAGAGTTCCATCTCAAGTGATTTCAAAGTTGCATAGATTAGAAGAATTGCGCATGCAATGTCAATTTAAGGGATGGCGTGGTAAAGGCGCGGGAGGCTTTTATTCTGTTCTTTTTTATAAGAATGCTGGCTTCGATGAGTTAACTGGCTTGTCACGTTTAAACCATTTGCATGTATCCCTAGCTGGTTCAGAATGCTTGCCTAAAGCTATCGAGTTTGATCCAAATTGGGTAAGCTTTAATATATGTATCAACAGAGGCTGGTTGTTCGGTAATCCCACCTTGCTACGTGACTATCGTTACAATCATTCCAGAGTCGTGACTCTTGACACAAGCATCAATACATTACCGGATTGGTTTATCAATGTGGTGACAAAGAAAGCGGAGACGCTATTTTATTATGACCATGGAAGACTCCAGAGATTGAAGTATCTCTGTGTACAATATGACGAGAACCTGAAAGAGTTGATGAACACAACAGCACGACTTTCAAATGAACCTGTATTTCAGAGTTTGGAAGATTTGTATCTCTCTGACTTAGCTTGCTTGGATAAATTATGTGTAGGTGAATTACCACCTGGGTCTCTATGTAATCTGAAGATATTGGAGATCACAGAGACTACAAAGTCTGGAAAAACTAGATTGTTCGAATATAGAGAAGCTGGAATATGTGTTTGGATTTGA

Protein Analysis

912

Amino Acids

103.88

Weight (kDa)

6.78

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 187 1.4e-48 TIR domain
TIR_2 PF13676 21 - 132 9.1e-15 TIR domain
NB-ARC PF00931 206 - 371 1e-32 NB-ARC domain
LRR_8 PF13855 580 - 638 2.8e-08 Leucine rich repeat
LRR_14 PF23598 639 - 707 5.8e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 498, 2175
AasI GACNNNNNNGTC 1 cut(s) 2680
AatII GACGTC 1 cut(s) 2040
Acc16I TGCGCA 1 cut(s) 2108
AccII CGCG 2 cut(s) 1187, 2147
AciI CCGC 3 cut(s) 671, 2147, 2438
AclI AACGTT 1 cut(s) 852
AclWI GGATC 2 cut(s) 1230, 2270
AcoI YGGCCR 3 cut(s) 228, 465, 1652
AcsI RAATTY 5 cut(s) 321, 599, 954, 1133, 2028
AcuI CTGAAG 3 cut(s) 1211, 1967, 2672
AcyI GRCGYC 1 cut(s) 2037
AfaI GTAC 5 cut(s) 360, 552, 1301, 1490, 2496
AfiI CCNNNNNNNGG 1 cut(s) 1868
AflIII ACRYGT 2 cut(s) 754, 1549
AjnI CCWGG 2 cut(s) 395, 2632
AjuI GAANNNNNNNTTGG 7 cut(s) 241, 273, 1035, 1067, 1340, 1372, 2713
Alw21I GWGCWC 1 cut(s) 733
Alw26I GTCTC 7 cut(s) 203, 839, 1181, 1641, 2435, 2643, 2666
AlwI GGATC 2 cut(s) 1230, 2270
AlwNI CAGNNNCTG 2 cut(s) 17, 2316
AoxI GGCC 4 cut(s) 3, 228, 465, 1652
ApeKI GCWGC 4 cut(s) 11, 1244, 1368, 1514
ApoI RAATTY 5 cut(s) 321, 599, 954, 1133, 2028
Asp700I GAANNNNTTC 1 cut(s) 87
AspLEI GCGC 3 cut(s) 643, 2109, 2147
AspS9I GGNCC 2 cut(s) 4, 1038
AsuHPI GGTGA 6 cut(s) 92, 385, 643, 994, 2437, 2633
AsuII TTCGAA 1 cut(s) 2703
AsuNHI GCTAGC 2 cut(s) 385, 1160
AvaII GGWCC 1 cut(s) 1038
BalI TGGCCA 3 cut(s) 230, 467, 1654
BanII GRGCYC 2 cut(s) 184, 733
BarI GAAGNNNNNNTAC 2 cut(s) 362, 394
BbsI GAAGAC 6 cut(s) 90, 315, 704, 898, 1865, 2473
Bbv12I GWGCWC 1 cut(s) 733
BbvI GCAGC 4 cut(s) 23, 1256, 1355, 1501
BccI CCATC 9 cut(s) 239, 628, 1255, 1538, 1601, 1687, 1870, 2074, 2124
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 2 cut(s) 397, 2634
BciVI GTATCC 1 cut(s) 2242
BcoDI GTCTC 7 cut(s) 203, 839, 1181, 1641, 2435, 2643, 2666
BfaI CTAG 7 cut(s) 386, 824, 867, 1161, 1476, 2237, 2694
BfuI GTATCC 1 cut(s) 2242
BglII AGATCT 2 cut(s) 817, 1782
BisI GCNGC 4 cut(s) 12, 1245, 1369, 1515
BlsI GCNGC 4 cut(s) 13, 1246, 1370, 1516
Bme1390I CCNGG 2 cut(s) 397, 2634
Bme18I GGWCC 1 cut(s) 1038
BmgT120I GGNCC 2 cut(s) 4, 1038
BmiI GGNNCC 4 cut(s) 183, 348, 1039, 1040
BmrFI CCNGG 2 cut(s) 397, 2634
BmsI GCATC 7 cut(s) 29, 439, 628, 891, 1333, 1548, 2400
BmtI GCTAGC 2 cut(s) 389, 1164
BpiI GAAGAC 6 cut(s) 90, 315, 704, 898, 1865, 2473
BpmI CTGGAG 1 cut(s) 2456
Bpu14I TTCGAA 1 cut(s) 2703
BpuEI CTTGAG 5 cut(s) 811, 1277, 1900, 1976, 2055
BsaAI YACGTR 2 cut(s) 1552, 2345
BsaBI GATNNNNATC 2 cut(s) 1221, 2580
BsaHI GRCGYC 1 cut(s) 2037
BsaI GGTCTC 2 cut(s) 1181, 2643
BsaJI CCNNGG 4 cut(s) 266, 1032, 2461, 2633
BsaWI WCCGGW 2 cut(s) 584, 2404
Bsc4I CCNNNNNNNGG 1 cut(s) 1868
Bse1I ACTGG 3 cut(s) 1655, 2047, 2206
Bse3DI GCAATG 3 cut(s) 298, 660, 2120
Bse8I GATNNNNATC 2 cut(s) 1221, 2580
BseBI CCWGG 2 cut(s) 397, 2634
BseDI CCNNGG 4 cut(s) 266, 1032, 2461, 2633
BseGI GGATG 6 cut(s) 163, 643, 1549, 1563, 1982, 2135
BseJI GATNNNNATC 2 cut(s) 1221, 2580
BseLI CCNNNNNNNGG 1 cut(s) 1868
BseMI GCAATG 3 cut(s) 298, 660, 2120
BseMII CTCAG 3 cut(s) 618, 1692, 1800
BseNI ACTGG 3 cut(s) 1655, 2047, 2206
BseRI GAGGAG 1 cut(s) 857
BseXI GCAGC 4 cut(s) 23, 1256, 1355, 1501
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 1187, 2147
Bsh1285I CGRYCG 1 cut(s) 1185
BshFI GGCC 4 cut(s) 5, 230, 467, 1654
BsiEI CGRYCG 1 cut(s) 1185
BsiHKAI GWGCWC 1 cut(s) 733
BsiSI CCGG 2 cut(s) 585, 2405
BslFI GGGAC 1 cut(s) 1051
BslI CCNNNNNNNGG 1 cut(s) 1868
BsmAI GTCTC 7 cut(s) 203, 839, 1181, 1641, 2435, 2643, 2666
BsmBI CGTCTC 1 cut(s) 2435
BsmFI GGGAC 1 cut(s) 1051
BsmI GAATGC 5 cut(s) 389, 1050, 1816, 2185, 2255
BsnI GGCC 4 cut(s) 5, 230, 467, 1654
Bso31I GGTCTC 2 cut(s) 1181, 2643
Bsp119I TTCGAA 1 cut(s) 2703
Bsp1286I GDGCHC 2 cut(s) 184, 733
Bsp1407I TGTACA 3 cut(s) 1299, 1488, 2494
Bsp143I GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
Bsp19I CCATGG 3 cut(s) 266, 1032, 2461
BspACI CCGC 3 cut(s) 671, 2147, 2438
BspANI GGCC 4 cut(s) 5, 230, 467, 1654
BspCNI CTCAG 3 cut(s) 619, 1691, 1799
BspFNI CGCG 2 cut(s) 1187, 2147
BspLI GGNNCC 4 cut(s) 183, 348, 1039, 1040
BspOI GCTAGC 2 cut(s) 389, 1164
BspPI GGATC 2 cut(s) 1230, 2270
BspQI GCTCTTC 2 cut(s) 159, 853
BspT104I TTCGAA 1 cut(s) 2703
BspTNI GGTCTC 2 cut(s) 1181, 2643
BsrDI GCAATG 3 cut(s) 298, 660, 2120
BsrGI TGTACA 3 cut(s) 1299, 1488, 2494
BsrI ACTGG 3 cut(s) 1655, 2047, 2206
BssECI CCNNGG 4 cut(s) 266, 1032, 2461, 2633
BssMI GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
BssNI GRCGYC 1 cut(s) 2037
BssT1I CCWWGG 3 cut(s) 266, 1032, 2461
Bst2UI CCWGG 2 cut(s) 397, 2634
Bst4CI ACNGT 3 cut(s) 377, 658, 1198
Bst6I CTCTTC 2 cut(s) 159, 853
BstACI GRCGYC 1 cut(s) 2037
BstAPI GCANNNNNTGC 1 cut(s) 17
BstAUI TGTACA 3 cut(s) 1299, 1488, 2494
BstBAI YACGTR 2 cut(s) 1552, 2345
BstBI TTCGAA 1 cut(s) 2703
BstC8I GCNNGC 7 cut(s) 387, 1162, 1712, 2111, 2185, 2255, 2599
BstDEI CTNAG 6 cut(s) 627, 1678, 1786, 1825, 1964, 2593
BstDSI CCRYGG 3 cut(s) 266, 1032, 2461
BstF5I GGATG 6 cut(s) 163, 643, 1549, 1563, 1982, 2135
BstFNI CGCG 2 cut(s) 1187, 2147
BstHHI GCGC 3 cut(s) 643, 2109, 2147
BstKTI GATC 6 cut(s) 820, 1185, 1225, 1785, 2278, 2667
BstMAI GTCTC 7 cut(s) 203, 839, 1181, 1641, 2435, 2643, 2666
BstMBI GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
BstMCI CGRYCG 1 cut(s) 1185
BstMWI GCNNNNNNNGC 3 cut(s) 11, 17, 2151
BstNI CCWGG 2 cut(s) 397, 2634
BstNSI RCATGY 3 cut(s) 758, 2113, 2231
BstSCI CCNGG 2 cut(s) 395, 2632
BstUI CGCG 2 cut(s) 1187, 2147
BstV1I GCAGC 4 cut(s) 23, 1256, 1355, 1501
BstV2I GAAGAC 6 cut(s) 90, 315, 704, 898, 1865, 2473
BstX2I RGATCY 3 cut(s) 817, 1222, 1782
BstXI CCANNNNNNTGG 1 cut(s) 1726
BstYI RGATCY 3 cut(s) 817, 1222, 1782
BsuI GTATCC 1 cut(s) 2242
BsuRI GGCC 4 cut(s) 5, 230, 467, 1654
BtgI CCRYGG 3 cut(s) 266, 1032, 2461
BtsCI GGATG 6 cut(s) 163, 643, 1549, 1563, 1982, 2135
BtsI GCAGTG 1 cut(s) 446
BtsIMutI CAGTG 2 cut(s) 446, 2040
Cac8I GCNNGC 7 cut(s) 387, 1162, 1712, 2111, 2185, 2255, 2599
CaiI CAGNNNCTG 2 cut(s) 17, 2316
CfoI GCGC 3 cut(s) 643, 2109, 2147
Cfr13I GGNCC 2 cut(s) 4, 1038
CseI GACGC 2 cut(s) 445, 2452
Csp6I GTAC 5 cut(s) 359, 551, 1300, 1489, 2495
CspCI CAANNNNNGTGG 4 cut(s) 690, 725, 1137, 1172
CviQI GTAC 5 cut(s) 359, 551, 1300, 1489, 2495
DdeI CTNAG 6 cut(s) 627, 1678, 1786, 1825, 1964, 2593
DpnI GATC 6 cut(s) 819, 1184, 1224, 1784, 2277, 2666
DpnII GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
DraI TTTAAA 2 cut(s) 456, 2217
DrdI GACNNNNNNGTC 1 cut(s) 2680
DseDI GACNNNNNNGTC 1 cut(s) 2680
EaeI YGGCCR 3 cut(s) 228, 465, 1652
Eam1104I CTCTTC 2 cut(s) 159, 853
EarI CTCTTC 2 cut(s) 159, 853
Ecl136II GAGCTC 1 cut(s) 731
Eco130I CCWWGG 3 cut(s) 266, 1032, 2461
Eco24I GRGCYC 2 cut(s) 184, 733
Eco31I GGTCTC 2 cut(s) 1181, 2643
Eco32I GATATC 1 cut(s) 1393
Eco47I GGWCC 1 cut(s) 1038
Eco53kI GAGCTC 1 cut(s) 731
Eco57I CTGAAG 3 cut(s) 1211, 1967, 2672
EcoICRI GAGCTC 1 cut(s) 731
EcoO109I RGGNCCY 1 cut(s) 1038
EcoRI GAATTC 3 cut(s) 321, 954, 1133
EcoRII CCWGG 2 cut(s) 395, 2632
EcoRV GATATC 1 cut(s) 1393
EcoT14I CCWWGG 3 cut(s) 266, 1032, 2461
EcoT22I ATGCAT 2 cut(s) 1563, 1816
EcoT38I GRGCYC 2 cut(s) 184, 733
ErhI CCWWGG 3 cut(s) 266, 1032, 2461
Esp3I CGTCTC 1 cut(s) 2435
FalI AAGNNNNNCTT 2 cut(s) 1340, 1372
FaqI GGGAC 1 cut(s) 1051
FauI CCCGC 1 cut(s) 2140
Fnu4HI GCNGC 4 cut(s) 12, 1245, 1369, 1515
FokI GGATG 6 cut(s) 170, 650, 1556, 1570, 1989, 2142
FriOI GRGCYC 2 cut(s) 184, 733
Fsp4HI GCNGC 4 cut(s) 12, 1245, 1369, 1515
FspBI CTAG 7 cut(s) 386, 824, 867, 1161, 1476, 2237, 2694
FspI TGCGCA 1 cut(s) 2108
GlaI GCGC 3 cut(s) 642, 2108, 2146
GluI GCNGC 4 cut(s) 12, 1245, 1369, 1515
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 2456
HaeIII GGCC 4 cut(s) 5, 230, 467, 1654
HapII CCGG 2 cut(s) 585, 2405
HgaI GACGC 2 cut(s) 445, 2452
HhaI GCGC 3 cut(s) 643, 2109, 2147
Hin1I GRCGYC 1 cut(s) 2037
Hin6I GCGC 3 cut(s) 641, 2107, 2145
HinP1I GCGC 3 cut(s) 641, 2107, 2145
HincII GTYRAC 3 cut(s) 582, 1450, 2199
HindII GTYRAC 3 cut(s) 582, 1450, 2199
HindIII AAGCTT 3 cut(s) 1405, 1712, 2290
HinfI GANTC 9 cut(s) 29, 218, 533, 1079, 1790, 1960, 2370, 2377, 2469
HpaI GTTAAC 1 cut(s) 2199
HpaII CCGG 2 cut(s) 585, 2405
HphI GGTGA 6 cut(s) 92, 385, 643, 994, 2437, 2633
Hpy166II GTNNAC 8 cut(s) 532, 582, 725, 1300, 1450, 2048, 2199, 2495
Hpy8I GTNNAC 8 cut(s) 532, 582, 725, 1300, 1450, 2048, 2199, 2495
Hpy99I CGWCG 3 cut(s) 48, 521, 1638
HpyAV CCTTC 3 cut(s) 454, 832, 871
HpyCH4III ACNGT 3 cut(s) 377, 658, 1198
HpyCH4IV ACGT 7 cut(s) 87, 852, 1551, 2037, 2050, 2212, 2344
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 17, 2151
HpyF3I CTNAG 6 cut(s) 627, 1678, 1786, 1825, 1964, 2593
HpySE526I ACGT 7 cut(s) 87, 852, 1551, 2037, 2050, 2212, 2344
Hsp92I GRCGYC 1 cut(s) 2037
HspAI GCGC 3 cut(s) 641, 2107, 2145
KflI GGGWCCC 1 cut(s) 1038
KspAI GTTAAC 1 cut(s) 2199
Kzo9I GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
LguI GCTCTTC 2 cut(s) 159, 853
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 4 cut(s) 23, 1256, 1355, 1501
LweI GCATC 7 cut(s) 29, 439, 628, 891, 1333, 1548, 2400
MaeI CTAG 7 cut(s) 386, 824, 867, 1161, 1476, 2237, 2694
MaeII ACGT 7 cut(s) 87, 852, 1551, 2037, 2050, 2212, 2344
MaeIII GTNAC 7 cut(s) 377, 2038, 2208, 2345, 2354, 2374, 2425
MalI GATC 6 cut(s) 819, 1184, 1224, 1784, 2277, 2666
MboI GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
MfeI CAATTG 1 cut(s) 1590
MflI RGATCY 3 cut(s) 817, 1222, 1782
MhlI GDGCHC 2 cut(s) 184, 733
MlsI TGGCCA 3 cut(s) 230, 467, 1654
MluNI TGGCCA 3 cut(s) 230, 467, 1654
MlyI GAGTC 4 cut(s) 527, 2371, 2379, 2463
MmeI TCCRAC 3 cut(s) 775, 835, 1192
Mox20I TGGCCA 3 cut(s) 230, 467, 1654
Mph1103I ATGCAT 2 cut(s) 1563, 1816
MroXI GAANNNNTTC 1 cut(s) 87
MscI TGGCCA 3 cut(s) 230, 467, 1654
MslI CAYNNNNRTG 1 cut(s) 2226
Msp20I TGGCCA 3 cut(s) 230, 467, 1654
MspA1I CMGCKG 2 cut(s) 353, 1247
MspI CCGG 2 cut(s) 585, 2405
MspR9I CCNGG 2 cut(s) 397, 2634
MssI GTTTAAAC 1 cut(s) 2217
MunI CAATTG 1 cut(s) 1590
Mva1269I GAATGC 5 cut(s) 389, 1050, 1816, 2185, 2255
MvaI CCWGG 2 cut(s) 397, 2634
MvnI CGCG 2 cut(s) 1187, 2147
MwoI GCNNNNNNNGC 3 cut(s) 11, 17, 2151
NcoI CCATGG 3 cut(s) 266, 1032, 2461
NdeII GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
NheI GCTAGC 2 cut(s) 385, 1160
NlaIV GGNNCC 4 cut(s) 183, 348, 1039, 1040
NmuCI GTSAC 5 cut(s) 2038, 2208, 2345, 2374, 2425
NsbI TGCGCA 1 cut(s) 2108
NsiI ATGCAT 2 cut(s) 1563, 1816
NspI RCATGY 3 cut(s) 758, 2113, 2231
NspV TTCGAA 1 cut(s) 2703
PaeI GCATGC 1 cut(s) 2113
PciI ACATGT 1 cut(s) 754
PciSI GCTCTTC 2 cut(s) 159, 853
PcsI WCGNNNNNNNCGW 1 cut(s) 2350
PctI GAATGC 5 cut(s) 389, 1050, 1816, 2185, 2255
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 5 cut(s) 29, 218, 1079, 1790, 1960
PflFI GACNNNGTC 1 cut(s) 656
PkrI GCNGC 4 cut(s) 13, 1246, 1370, 1516
Ple19I CGATCG 1 cut(s) 1185
PleI GAGTC 4 cut(s) 527, 2371, 2378, 2463
PmeI GTTTAAAC 1 cut(s) 2217
PpsI GAGTC 4 cut(s) 527, 2371, 2378, 2463
Ppu21I YACGTR 2 cut(s) 1552, 2345
PpuMI RGGWCCY 1 cut(s) 1038
PscI ACATGT 1 cut(s) 754
PsiI TTATAA 2 cut(s) 498, 2175
Psp124BI GAGCTC 1 cut(s) 733
Psp1406I AACGTT 1 cut(s) 852
Psp5II RGGWCCY 1 cut(s) 1038
Psp6I CCWGG 2 cut(s) 395, 2632
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 2 cut(s) 395, 2632
PspN4I GGNNCC 4 cut(s) 183, 348, 1039, 1040
PspPI GGNCC 2 cut(s) 4, 1038
PspPPI RGGWCCY 1 cut(s) 1038
PstNI CAGNNNCTG 2 cut(s) 17, 2316
PsuI RGATCY 3 cut(s) 817, 1222, 1782
PsyI GACNNNGTC 1 cut(s) 656
PvuI CGATCG 1 cut(s) 1185
PvuII CAGCTG 2 cut(s) 353, 1247
RsaI GTAC 5 cut(s) 360, 552, 1301, 1490, 2496
RsaNI GTAC 5 cut(s) 359, 551, 1300, 1489, 2495
RseI CAYNNNNRTG 1 cut(s) 2226
SacI GAGCTC 1 cut(s) 733
SapI GCTCTTC 2 cut(s) 159, 853
SatI GCNGC 4 cut(s) 12, 1245, 1369, 1515
Sau3AI GATC 6 cut(s) 817, 1182, 1222, 1782, 2275, 2664
Sau96I GGNCC 2 cut(s) 4, 1038
SchI GAGTC 4 cut(s) 527, 2371, 2379, 2463
ScrFI CCNGG 2 cut(s) 397, 2634
SduI GDGCHC 2 cut(s) 184, 733
SfaNI GCATC 7 cut(s) 29, 439, 628, 891, 1333, 1548, 2400
SfuI TTCGAA 1 cut(s) 2703
SinI GGWCC 1 cut(s) 1038
SmiMI CAYNNNNRTG 1 cut(s) 2226
SmlI CTYRAG 5 cut(s) 790, 1256, 1915, 1955, 2070
SmoI CTYRAG 5 cut(s) 790, 1256, 1915, 1955, 2070
SphI GCATGC 1 cut(s) 2113
SsiI CCGC 3 cut(s) 671, 2147, 2438
SspI AATATT 1 cut(s) 799
SspMI CTAG 7 cut(s) 386, 824, 867, 1161, 1476, 2237, 2694
SstI GAGCTC 1 cut(s) 733
StyD4I CCNGG 2 cut(s) 395, 2632
StyI CCWWGG 3 cut(s) 266, 1032, 2461
TaaI ACNGT 3 cut(s) 377, 658, 1198
TaiI ACGT 7 cut(s) 90, 855, 1554, 2040, 2053, 2215, 2347
TaqI TCGA 7 cut(s) 213, 519, 1441, 1573, 2190, 2268, 2703
TaqII GACCGA 1 cut(s) 1859
TatI WGTACW 3 cut(s) 1299, 1488, 2494
TfiI GAWTC 5 cut(s) 29, 218, 1079, 1790, 1960
TscAI CASTG 2 cut(s) 446, 2047
TseFI GTSAC 5 cut(s) 2038, 2208, 2345, 2374, 2425
TseI GCWGC 4 cut(s) 11, 1244, 1368, 1514
Tsp45I GTSAC 5 cut(s) 2038, 2208, 2345, 2374, 2425
TspDTI ATGAA 8 cut(s) 24, 135, 177, 287, 417, 1839, 2540, 2565
TspGWI ACGGA 1 cut(s) 518
TspRI CASTG 2 cut(s) 446, 2047
Tth111I GACNNNGTC 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 1038
XapI RAATTY 5 cut(s) 321, 599, 954, 1133, 2028
XceI RCATGY 3 cut(s) 758, 2113, 2231
XcmI CCANNNNNNNNNTGG 1 cut(s) 1489
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 7 cut(s) 386, 824, 867, 1161, 1476, 2237, 2694
ZraI GACGTC 1 cut(s) 2038
Zsp2I ATGCAT 2 cut(s) 1563, 1816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.