Rroxscaffold_1G00003040

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000085
Physical Location & Seq
Forward (+)
3536025 .. 3549290
13266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003040.1

Sequence Viewer

Length: 5742 bp
ATGAAAGACGACAACAGAATTCTGCCACTTTTTTATGATGTGGAACCTTCTGATGTATGGTACCGGAAGAGGAGCTTCTCCGAAGCTTTCAATAAGCATGAAAGTTCTGGGCGACACACATCAGACAAGGTGCAGCGGTGGAATGATGCTTTAAACAAAGCGGCCAGTTTCTCTGGCTGGAATTCGCAGGATTATAAGACTCAGAAAGAACTTGTTGACGACATTGTGGGATTTCTCGGCAGTAAAGTACTACCACCTGATGCAATTGAGTCCACAGGAGATTTTGAAGAATATGAAGCAACAAGACAAGCCATGGATGAGGTTATGAAGGCGCTAACAGAAGACGAGGTCACTGCCGTTGGTGTCTACGGCATGCGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAAAATTGGTACTTTTAACCATTGGATTATGGCTGCCATATCCGAAAGCCCTGACTACAGAAAAGTTCAAGGCACATTGGCAGACCTATTGGGCATCAAATTGATGGAGGAGACAGAAGATGGAAGAGCAGCTAGGTTGCATAAGGAGATAATGAGTAGAGAAAAACTCCTGATAATCTTGGACGATGTTTGGAATAGAACAGAATTGTCAACCATAGGAATTCCCAGCTACGAGGAGCTTCAAAAGTGCAGATCCAAAGTCTTACTCACCACACGGAGAAGGAATGTCTGTCATGTCATGAAGTGCCAAGCAAGCATTCCTCTCAATATCTTATCAGAACAAGATTCTTGGACCTTGTTTGTGAGAAATACAAGGTCTTTTGAATGGACCACTTTTGAGGGTGTAGCGAGGAAGGTAGCTGGAGAATGTGCTGGTCTACCGATTGCATTGAGAGCAGTTGCAAGGGCACTCAGAGGTAAGAACCTGCAGGAATGGGAAAAAGCAGCTCAACAACTACAGGAGTCGCAAAAAGCAGCTCTGCAACTACAATCTGCCTACCCTGAAGATGAAGATGCATTCAGATGTATAAAATCAAGCTATGATTTCAGATGTATAAAATCAAGCTATGATTGCTTGAAAGATGAAGATTCCAAGTCATGCTTCTTGCTTTGTTGCCTCTTTCCTGCAGACTATGACATCCCAATAGAAGACTTATCCAGGTATGCAATCGGGACAGGATTGTTTCTACGAGATCCCGAAACAATCGAAGAAGCCAGAGCAACAGCAGAATTAGTGGTCGGGAACCTGAAAGATCATAGGTTGCTTTTGGATAGTGAGAAAAAGGGATGTGTAAGGATGCATGAGGTCATTCGGGATGCAGCCCTGAATATTGCAAAATCTATAGATGGGGACGGGTTTCTGGTAAAAGCTGGCTGTGGTTTAGAGGATTGGCCATCTGGATCACTTCAAGAATACTCCGTAATCTCACTAACGTGCAACGAAATTAGCAAGCTGCCCGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAACCGAAACTATTATTTAACTGAGATCCCAGAAAAGTTTATCCAAAGTCTGAATGAATTAAGGGTCTTGGATCTTAGCGACACTTCTATTTCCTCTCTGCCCCAATCATTCAGCCTCCTTACCAACCTCCAAGTTTTATATTTAGATTTTTGCAAGAAATTGATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTAAGTATGAAGGAATATCCTGGGCGGGAATTGTCGAGAGAAATAGGACATTTAACCAACCTAAGGATTTTGGATATCAGTGGCGCTTGGGGTGGAGGTATAGTCACAATTCCATCTAAAGTGATGTCAAAGTTGCATAAATTAGAAGAATTGTACATGCTGTATTGTGGATTTGAGGACTGGGGGTGTGAAGTTGAGGGACAAGGAGGAGAAACCAATATTGGGTTTGATGAATTAGCTGGTTTATCAAATTTGAAAATTTTGCAGGTTTGCATATCTGATGCAAAATACATCCCTAAAAATGTTGAGGCCAAACCGAATTGGGTTTGCTTTGATATACGTATTGGCGGATGCACAGAAGATGTATACAATCGAGAAGATCATAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATAAGTTCCTTGCCTGATTGGTTTATCAACGTGGTGACAAAGAAAACAGAGAAGTTAGAGTATGATTGCTGCAAAGGGATGAGTGACATTCTTATGGAATATGACCATGGGCTGTGTATTGGTGAGTTCCCACCTGAGTCTCTCTGGAATCTCAAGGTATTGCATATGTTTGGTTGTAGTATCTTGAAGAATGTATCAAAATTTATACAGAGACTACCAAATCTAGAGAAACTATGTTTAAATTGGATGGCTGAATTGGAATATGTGTTTGGATGCGAAGGGTTTGAGCCGAAACAATCAAAACTGAGAGAGATTCATTTGTCGGGTCTAAATTCAGTGAAGAGCATTTGTCATGGTCCTGCCCCATGCGTAATATTCCAGAGTCTTAAAAGTTTGACCTTTTTCCGTTGCGAGTTGCTGCAAAGTCTGTTCGCATGTGATGTAGCCCAGTGTCTCTTTCAATTGGAAGACCTTTTTGTAGAGCAGTGCCCTTTGTTGGAAAGAGTAATTGAAGCAGTGAACAACGAGAAGATGGTTCTACCAAAATTGAAGAACTTGGTTTTGAAGAATCTTCCTATGTTGTATGGTGCAAGTGCTACTGTTGATATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGCCACCAGTTTTCATTTTTAACCTCTTCGTCTCCCTTCAGAGCCTTCGAAAGCAGTAACACAGTCAAGTTCAATGATCCACAACTGTACAACCTGCTGTATGATAGGACTGAAGGACCAAAAAGGCAACTTGGGGGATCTGTGTTGAGTACAGTGCGACCTATTGAAACTGAGTTTACAATGCCCATGGGAGATTTTCAAGCATTTGAAGCAACAAGACAGGCCATGGATGAGGTTATGGAGGCACTAAAAGCTGATGAGGTCACAGCCATTGGGGTCTACGGAATGGGAGGAGTTGGAAAAACAACCATGGTTGAACATGTTAGTGCAGAAGTTCGGGATAATGGGATGTTTCATCATGTGATCACGGCTGTTGTATCCAAAAGCCCTGACTTGAGAAAAATTCAAGGAACATTGGCAGGTATGTTGGGTTTTATATTCGAGGAGGAGACAGAAATTGGAAGATCCCGTAGATTGATGCACAAGATAAGGAGAGGAAATAGGATCCTTATAATCTTGGACGACATGTGGGAGAGAATAGATTTGTCCAACATAGGAATTCCCAGCTACCACGAGCTTCAAAGATGCAATTCCAAAGTCCTAATCACCACAAGGACACGTGATGTTTGTTGTGCCATGGAGTGTCAATCAATCATTTTTCTTGATCGACTATCAGAAAAAGATTCTTTGACCTTGTTTGTGAACAAAGCAAAAATGTCTTCTGAATCGACCAATTTTTATGATGTTGCAAAGAAGGTAGCTAGAAAATGTGCTGGTCTTCCCGTTGCGCTGATAGCAGTTGCCAAGGCACTTGGTGATAAGGGTTTGGATTACTGGAAGGAAGCAGCTCGACGATTAAGGGCTTCTCAAACTGCCAACTCTGAAGATGGGAACACAATCAATTGTATAAAATTAAGCTATGATTACTTAGAATCTGATGATGCCAGGTCATGCTTCTTGCTTTGTTGTCTATTCCCAGAAGACTATGATATCCAAATTGAAGACTTGCTAAAATATGGGATGGGGAACGAATTGTTTCAAGATTCCAGGAGGACCAGGACGATGCATGATGCCAGAGCTAGTGCATATTCGGTGGTCAAGTACCTTAAAGATTGTAGCTTGCTTTTGAAATCTGAACAGGACGGATGTGTAAAGATGCATGATGTCATTCGGGATGTGGCAATATTAATTTCTAAGGATGACCCTCTGTTTTTAGTAAAAGCTGGTTGTCAATTAAAGGAATGGCCAATTATGGGTGCAGAAGAAGGCTACTCTGCTATTTCAGTAATTAGGAATAAAATTTGCAAGCTTCCTGAAGAGTTGGTATGTCCGAAACTCCTTATTTTGTGTATGCAAAGTAATGTTGACTTGGATGAGATCCCAGAAAATTTCTTTCAAAGTTTGAATGTGTTGAGGGTATTAGATCTAAGCCGAACCAGAATTTCGTTATTACCCACATCATTCAATCGCTTGACGAACCTCCACACCTTGTATCTTGATGGTTGTTTATCAATCATAAATAGCATATCTGTACTCGGAAATTTAAAAAAACTTGAGATTCTTAGTATGAGAGACCTTCCTCTTGTGGAATTGCCAAAAGAAATAGGACATCTGAGTAATCTAAGGATGTTGGACATCACTGGTGAATATGTTGATACAGTTCCCTCTGAAGTGATTTGCAACCTTCATAGGTTAGAAGAATTATACATGCAGTGTAATTTTGCAGACTGGAGGAGTAAAGTTTGGAGGAATGAAGTGAATGAGAGAAAAGTTGATGAAGCAGGGGAAGAAAATATTGCTGCCTTTGATGACTTAACCAGCTTGTCATGTCTAAACATTTTGAAGGTTTTCATATCTGATGCAAAATGTTTGCCTACAGATGTTGAGGTTGAGCCGAGTTGGATAAACTTTGATATATGTATTGGCAGAAATCCGTCAATTAGAAAAGTGTCCTTGGTTTCAAGTTCTTCACCTGCTTATACAAGATCTTTGACTCTTGGTACAACCATGAATGCCTTACCGAGATGGTTTATCAATGTGGTGACAGGGAAAGCAGAGAAACTACAGTACATAATGTGTTGGGGATTAGATAACATTCTTGCGGAATATGACCGTGGGAGATTACATGCACTGAAGTATTTTTCTGTTATTGGGCCCAGTGAGAACTTGAAAGTCTTGATGAACACAAAAACATGGGTTCGAAATGAACCTGTGTTTCAGAACTTAGAAGAGTTGCATCTCTTTCAAGTGGATAGCCTGAAGGAATTATGTGTTGGTGAATTACCACTTGGGTCTCTATGCAATCTTAGGTTATTGAAGGTGAAACATTGTTACGAATTGGTGGATGCACTTCTATCGTCACAATTGCTGCAGAGATTACAAAATCTGGAAACACTGATTTGTGAAGAGATGGATGAATTGGAATATGTTTTTGGATGTGAAGGGCTTAAGCCAGAACATATTATTTTGACAGAATTGAAAGAGATGAGATTGGATAATCTGGGGAAACTAATAAAGATATGGAATGGTCCTGCTCCTTGTGCCATTTTCCAAAACCTTCAGAGTTTGGTAGTTTCAGGATGCTTTGAATTGGAAAATCTCTTCACCGCTGATGTAGCTCAGTGTCTTTTGCAATTGGAAGACCTTTCAGTAGAGCTTTGCCCATTATTGGACAGAGTTATTGAGGCAAGCAAGGAAACAATAGACAGCAAGATTGTTTTTCCAAAACTGAAGAACTTAGCTTTGATAAAGCTACCACAGCTCACAAGGTTCTATGGTAATACAGGAAGTGCTATTGAGTGCCCTTTATTAGAATACTTGTATGTGGAGAGGTGCCCCCAGTTCTCAACATCTGACTTCTACAGCAGGAACAAAGTTCCAACTCAATCATCAATAACATTTGGACATTGTACAAAGAAGATTGCTGAGAAACAAAAGAAGGCCCTGATGGGAACAGAAGAAGCAGAAAGAGATATTCGATGGTCTTTTCGATGA

Protein Analysis

1913

Amino Acids

216.57

Weight (kDa)

5.4

Isoelectric Point (pI)

48.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 5 - 77 4.7e-08 TIR domain
NB-ARC PF00931 103 - 263 4e-27 NB-ARC domain
LRR_8 PF13855 487 - 545 2.3e-10 Leucine rich repeat
LRR_14 PF23598 489 - 618 2.7e-17 Leucine-rich repeat region
LRR_RPS2 PF23247 740 - 864 3.1e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
NB-ARC PF00931 990 - 1156 1.4e-26 NB-ARC domain
LRR_8 PF13855 1369 - 1418 4.6e-06 Leucine rich repeat
LRR_14 PF23598 1370 - 1439 6.6e-07 Leucine-rich repeat region
LRR_14 PF23598 1413 - 1489 3.6e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 1658 - 1803 8.9e-28 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 195, 3260
AarI CACCTGC 1 cut(s) 4729
Acc36I ACCTGC 5 cut(s) 918, 1942, 2850, 3158, 4729
Acc65I GGTACC 1 cut(s) 60
AccB1I GGYRCC 2 cut(s) 60, 5580
AccI GTMKAC 4 cut(s) 366, 862, 2052, 3027
AccII CGCG 1 cut(s) 378
AciI CCGC 7 cut(s) 136, 161, 378, 1711, 2034, 4850, 5355
AcoI YGGCCR 3 cut(s) 162, 1376, 3992
AcvI CACGTG 1 cut(s) 3368
AdeI CACNNNGTG 1 cut(s) 3563
AfiI CCNNNNNNNGG 5 cut(s) 1749, 1908, 2599, 4001, 5416
AflII CTTAAG 1 cut(s) 5195
AflIII ACRYGT 4 cut(s) 406, 3067, 3273, 3365
AjnI CCWGG 5 cut(s) 1142, 1705, 3692, 3794, 3803
AleI CACNNNNGTG 1 cut(s) 1417
Alw26I GTCTC 9 cut(s) 397, 530, 2247, 2308, 2561, 2784, 3191, 4314, 5046
AoxI GGCC 7 cut(s) 162, 1376, 1995, 2970, 3992, 4901, 5688
ApaI GGGCCC 1 cut(s) 4905
AseI ATTAAT 1 cut(s) 3935
Asp700I GAANNNNTTC 2 cut(s) 3783, 4595
Asp718I GGTACC 1 cut(s) 60
AspLEI GCGC 4 cut(s) 334, 378, 1772, 3538
AspS9I GGNCC 9 cut(s) 777, 813, 2459, 2864, 3801, 4901, 4902, 5276, 5689
AsuII TTCGAA 2 cut(s) 2796, 4948
AvaII GGWCC 6 cut(s) 777, 813, 2459, 2864, 3801, 5276
AxyI CCTNAGG 1 cut(s) 1748
BaeGI GKGCMC 5 cut(s) 895, 2594, 4905, 5552, 5585
BalI TGGCCA 2 cut(s) 1378, 3994
BamHI GGATCC 1 cut(s) 3252
BanI GGYRCC 2 cut(s) 60, 5580
BanII GRGCYC 1 cut(s) 4905
BauI CACGAG 1 cut(s) 3320
BbrPI CACGTG 1 cut(s) 3368
BbsI GAAGAC 8 cut(s) 348, 1140, 2577, 3459, 3518, 3735, 3756, 5394
BceAI ACGGC 3 cut(s) 341, 385, 3132
BcgI CGANNNNNNTGC 4 cut(s) 335, 369, 5085, 5119
BciT130I CCWGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
BciVI GTATCC 1 cut(s) 3136
BclI TGATCA 1 cut(s) 3111
BcoDI GTCTC 9 cut(s) 397, 530, 2247, 2308, 2561, 2784, 3191, 4314, 5046
BfaI CTAG 4 cut(s) 558, 2327, 3510, 3828
BfoI RGCGCY 2 cut(s) 335, 1773
BfrI CTTAAG 1 cut(s) 5195
BfuAI ACCTGC 5 cut(s) 918, 1942, 2850, 3158, 4729
BfuI GTATCC 1 cut(s) 3136
BglII AGATCT 2 cut(s) 4171, 4733
BmcAI AGTACT 1 cut(s) 249
Bme1390I CCNGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
Bme18I GGWCC 6 cut(s) 777, 813, 2459, 2864, 3801, 5276
BmgT120I GGNCC 9 cut(s) 777, 813, 2459, 2864, 3801, 4901, 4902, 5276, 5689
BmiI GGNNCC 6 cut(s) 45, 62, 1229, 3254, 4903, 5582
BmrFI CCNGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
BmrI ACTGGG 4 cut(s) 1876, 2545, 4899, 5581
BmuI ACTGGG 4 cut(s) 1876, 2545, 4899, 5581
BpiI GAAGAC 8 cut(s) 348, 1140, 2577, 3459, 3518, 3735, 3756, 5394
BplI GAGNNNNNCTC 2 cut(s) 576, 608
BpmI CTGGAG 3 cut(s) 867, 1451, 4498
Bpu14I TTCGAA 2 cut(s) 2796, 4948
BpuEI CTTGAG 3 cut(s) 2240, 3163, 4322
BsaAI YACGTR 2 cut(s) 2027, 3368
BsaI GGTCTC 2 cut(s) 4314, 5046
BsaWI WCCGGW 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 1773, 1803
Bsc4I CCNNNNNNNGG 5 cut(s) 1749, 1908, 2599, 4001, 5416
Bse1I ACTGG 9 cut(s) 165, 1871, 2551, 2755, 3587, 4393, 4481, 4905, 5587
Bse21I CCTNAGG 1 cut(s) 1748
BseBI CCWGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
BseLI CCNNNNNNNGG 5 cut(s) 1749, 1908, 2599, 4001, 5416
BseMII CTCAG 9 cut(s) 215, 910, 1494, 2229, 2399, 2910, 4352, 5381, 5664
BseNI ACTGG 9 cut(s) 165, 1871, 2551, 2755, 3587, 4393, 4481, 4905, 5587
BseRI GAGGAG 8 cut(s) 85, 548, 674, 1908, 3054, 3206, 3209, 4495
BseSI GKGCMC 5 cut(s) 895, 2594, 4905, 5552, 5585
BseYI CCCAGC 2 cut(s) 650, 3311
BsgI GTGCAG 5 cut(s) 152, 399, 694, 3096, 4026
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 7 cut(s) 164, 1378, 1997, 2972, 3994, 4903, 5690
BshNI GGYRCC 2 cut(s) 60, 5580
BsiSI CCGG 1 cut(s) 64
BslFI GGGAC 3 cut(s) 1171, 1349, 1899
BslI CCNNNNNNNGG 5 cut(s) 1749, 1908, 2599, 4001, 5416
BsmAI GTCTC 9 cut(s) 397, 530, 2247, 2308, 2561, 2784, 3191, 4314, 5046
BsmBI CGTCTC 1 cut(s) 2784
BsmFI GGGAC 3 cut(s) 1171, 1349, 1899
BsmI GAATGC 3 cut(s) 741, 1001, 4765
BsnI GGCC 7 cut(s) 164, 1378, 1997, 2972, 3994, 4903, 5690
Bso31I GGTCTC 2 cut(s) 4314, 5046
Bsp119I TTCGAA 2 cut(s) 2796, 4948
Bsp120I GGGCCC 1 cut(s) 4901
Bsp1286I GDGCHC 5 cut(s) 895, 2594, 4905, 5552, 5585
Bsp1407I TGTACA 3 cut(s) 1839, 2835, 5657
Bsp19I CCATGG 6 cut(s) 312, 2209, 2934, 2973, 3057, 3384
BspACI CCGC 7 cut(s) 136, 161, 378, 1711, 2034, 4850, 5355
BspANI GGCC 7 cut(s) 164, 1378, 1997, 2972, 3994, 4903, 5690
BspCNI CTCAG 9 cut(s) 214, 909, 1495, 2230, 2400, 2911, 4353, 5380, 5665
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 723
BspLI GGNNCC 6 cut(s) 45, 62, 1229, 3254, 4903, 5582
BspMAI CTGCAG 3 cut(s) 915, 1114, 5121
BspMI ACCTGC 5 cut(s) 918, 1942, 2850, 3158, 4729
BspQI GCTCTTC 2 cut(s) 544, 2438
BspT104I TTCGAA 2 cut(s) 2796, 4948
BspT107I GGYRCC 2 cut(s) 60, 5580
BspTI CTTAAG 1 cut(s) 5195
BspTNI GGTCTC 2 cut(s) 4314, 5046
BsrGI TGTACA 3 cut(s) 1839, 2835, 5657
BsrI ACTGG 9 cut(s) 165, 1871, 2551, 2755, 3587, 4393, 4481, 4905, 5587
BssNAI GTATAC 1 cut(s) 2053
BssSI CACGAG 1 cut(s) 3320
BssT1I CCWWGG 8 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 3552, 4701
Bst1107I GTATAC 1 cut(s) 2053
Bst2BI CACGAG 1 cut(s) 3320
Bst2UI CCWGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
Bst4CI ACNGT 7 cut(s) 2704, 2812, 2835, 2902, 4408, 4815, 4862
Bst6I CTCTTC 9 cut(s) 62, 544, 1440, 2438, 2779, 4059, 4971, 5148, 5354
BstAFI CTTAAG 1 cut(s) 5195
BstAUI TGTACA 3 cut(s) 1839, 2835, 5657
BstBAI YACGTR 2 cut(s) 2027, 3368
BstBI TTCGAA 2 cut(s) 2796, 4948
BstC8I GCNNGC 8 cut(s) 374, 424, 739, 1357, 1436, 3869, 4055, 5437
BstDSI CCRYGG 7 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 4861
BstFNI CGCG 1 cut(s) 378
BstH2I RGCGCY 2 cut(s) 335, 1773
BstHHI GCGC 4 cut(s) 334, 378, 1772, 3538
BstMAI GTCTC 9 cut(s) 397, 530, 2247, 2308, 2561, 2784, 3191, 4314, 5046
BstNI CCWGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
BstNSI RCATGY 8 cut(s) 376, 410, 1846, 2541, 3071, 3277, 4459, 4877
BstSCI CCNGG 5 cut(s) 1142, 1705, 3692, 3794, 3803
BstSLI GKGCMC 5 cut(s) 895, 2594, 4905, 5552, 5585
BstSNI TACGTA 1 cut(s) 2027
BstUI CGCG 1 cut(s) 378
BstV2I GAAGAC 8 cut(s) 348, 1140, 2577, 3459, 3518, 3735, 3756, 5394
BstZ17I GTATAC 1 cut(s) 2053
Bsu36I CCTNAGG 1 cut(s) 1748
BsuI GTATCC 1 cut(s) 3136
BsuRI GGCC 7 cut(s) 164, 1378, 1997, 2972, 3994, 4903, 5690
BtgI CCRYGG 7 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 4861
BtsI GCAGTG 4 cut(s) 351, 2594, 2625, 4466
BveI ACCTGC 5 cut(s) 918, 1942, 2850, 3158, 4729
Cac8I GCNNGC 8 cut(s) 374, 424, 739, 1357, 1436, 3869, 4055, 5437
CciI TCATGA 1 cut(s) 723
CfoI GCGC 4 cut(s) 334, 378, 1772, 3538
Cfr13I GGNCC 9 cut(s) 777, 813, 2459, 2864, 3801, 4901, 4902, 5276, 5689
DraI TTTAAA 3 cut(s) 153, 2343, 4293
DraIII CACNNNGTG 1 cut(s) 3563
EaeI YGGCCR 3 cut(s) 162, 1376, 3992
Eam1104I CTCTTC 9 cut(s) 62, 544, 1440, 2438, 2779, 4059, 4971, 5148, 5354
EarI CTCTTC 9 cut(s) 62, 544, 1440, 2438, 2779, 4059, 4971, 5148, 5354
EciI GGCGGA 1 cut(s) 2049
Eco105I TACGTA 1 cut(s) 2027
Eco130I CCWWGG 8 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 3552, 4701
Eco24I GRGCYC 1 cut(s) 4905
Eco31I GGTCTC 2 cut(s) 4314, 5046
Eco32I GATATC 2 cut(s) 1762, 3739
Eco47I GGWCC 6 cut(s) 777, 813, 2459, 2864, 3801, 5276
Eco72I CACGTG 1 cut(s) 3368
Eco81I CCTNAGG 1 cut(s) 1748
EcoO109I RGGNCCY 1 cut(s) 5689
EcoRI GAATTC 4 cut(s) 18, 181, 645, 3306
EcoRII CCWGG 5 cut(s) 1142, 1705, 3692, 3794, 3803
EcoRV GATATC 2 cut(s) 1762, 3739
EcoT14I CCWWGG 8 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 3552, 4701
EcoT22I ATGCAT 4 cut(s) 1003, 1287, 3816, 3909
EcoT38I GRGCYC 1 cut(s) 4905
ErhI CCWWGG 8 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 3552, 4701
Esp3I CGTCTC 1 cut(s) 2784
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 1070, 1102
FaqI GGGAC 3 cut(s) 1171, 1349, 1899
FauI CCCGC 1 cut(s) 1704
FauNDI CATATG 1 cut(s) 2268
FbaI TGATCA 1 cut(s) 3111
FblI GTMKAC 4 cut(s) 366, 862, 2052, 3027
FriOI GRGCYC 1 cut(s) 4905
FspBI CTAG 4 cut(s) 558, 2327, 3510, 3828
GlaI GCGC 4 cut(s) 333, 377, 1771, 3537
GsaI CCCAGC 2 cut(s) 654, 3315
GsuI CTGGAG 3 cut(s) 867, 1451, 4498
HaeII RGCGCY 2 cut(s) 335, 1773
HaeIII GGCC 7 cut(s) 164, 1378, 1997, 2972, 3994, 4903, 5690
HapII CCGG 1 cut(s) 64
HhaI GCGC 4 cut(s) 334, 378, 1772, 3538
Hin6I GCGC 4 cut(s) 332, 376, 1770, 3536
HinP1I GCGC 4 cut(s) 332, 376, 1770, 3536
HincII GTYRAC 3 cut(s) 217, 636, 4114
HindII GTYRAC 3 cut(s) 217, 636, 4114
HindIII AAGCTT 3 cut(s) 84, 1676, 4055
HpaII CCGG 1 cut(s) 64
Hpy99I CGWCG 1 cut(s) 3603
HpyCH4III ACNGT 7 cut(s) 2704, 2812, 2835, 2902, 4408, 4815, 4862
HpyCH4IV ACGT 4 cut(s) 1418, 2026, 2133, 3367
HpySE526I ACGT 4 cut(s) 1418, 2026, 2133, 3367
HspAI GCGC 4 cut(s) 332, 376, 1770, 3536
KpnI GGTACC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 3111
LguI GCTCTTC 2 cut(s) 544, 2438
LmnI GCTCC 4 cut(s) 72, 661, 2096, 5287
MaeI CTAG 4 cut(s) 558, 2327, 3510, 3828
MaeII ACGT 4 cut(s) 1418, 2026, 2133, 3367
MaeIII GTNAC 9 cut(s) 349, 1789, 2137, 2186, 2804, 3010, 4789, 5078, 5106
MfeI CAATTG 5 cut(s) 264, 2564, 3649, 5111, 5381
MhlI GDGCHC 5 cut(s) 895, 2594, 4905, 5552, 5585
MlsI TGGCCA 2 cut(s) 1378, 3994
MluNI TGGCCA 2 cut(s) 1378, 3994
MlyI GAGTC 6 cut(s) 193, 278, 956, 2249, 2494, 4735
MmeI TCCRAC 7 cut(s) 364, 2580, 3025, 3321, 4359, 4628, 5651
Mox20I TGGCCA 2 cut(s) 1378, 3994
Mph1103I ATGCAT 4 cut(s) 1003, 1287, 3816, 3909
MroXI GAANNNNTTC 2 cut(s) 3783, 4595
MscI TGGCCA 2 cut(s) 1378, 3994
MslI CAYNNNNRTG 4 cut(s) 1006, 1417, 2195, 3072
Msp20I TGGCCA 2 cut(s) 1378, 3994
MspA1I CMGCKG 2 cut(s) 136, 5357
MspCI CTTAAG 1 cut(s) 5195
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
MunI CAATTG 5 cut(s) 264, 2564, 3649, 5111, 5381
Mva1269I GAATGC 3 cut(s) 741, 1001, 4765
MvaI CCWGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
MvnI CGCG 1 cut(s) 378
NcoI CCATGG 6 cut(s) 312, 2209, 2934, 2973, 3057, 3384
NdeI CATATG 1 cut(s) 2268
NlaIV GGNNCC 6 cut(s) 45, 62, 1229, 3254, 4903, 5582
NmeAIII GCCGAG 2 cut(s) 216, 4668
NmuCI GTSAC 7 cut(s) 349, 1789, 2137, 2186, 3010, 4789, 5106
NsiI ATGCAT 4 cut(s) 1003, 1287, 3816, 3909
NspI RCATGY 8 cut(s) 376, 410, 1846, 2541, 3071, 3277, 4459, 4877
NspV TTCGAA 2 cut(s) 2796, 4948
OliI CACNNNNGTG 1 cut(s) 1417
PaeI GCATGC 1 cut(s) 376
PagI TCATGA 1 cut(s) 723
PaqCI CACCTGC 1 cut(s) 4729
PciI ACATGT 3 cut(s) 406, 3067, 3273
PciSI GCTCTTC 2 cut(s) 544, 2438
PctI GAATGC 3 cut(s) 741, 1001, 4765
PdmI GAANNNNTTC 2 cut(s) 3783, 4595
PflFI GACNNNGTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 3794
PleI GAGTC 6 cut(s) 193, 277, 955, 2248, 2493, 4735
PmaCI CACGTG 1 cut(s) 3368
PmlI CACGTG 1 cut(s) 3368
PpsI GAGTC 6 cut(s) 193, 277, 955, 2248, 2493, 4735
Ppu21I YACGTR 2 cut(s) 2027, 3368
PscI ACATGT 3 cut(s) 406, 3067, 3273
PshBI ATTAAT 1 cut(s) 3935
PsiI TTATAA 2 cut(s) 195, 3260
Psp6I CCWGG 5 cut(s) 1142, 1705, 3692, 3794, 3803
PspCI CACGTG 1 cut(s) 3368
PspFI CCCAGC 2 cut(s) 650, 3311
PspGI CCWGG 5 cut(s) 1142, 1705, 3692, 3794, 3803
PspN4I GGNNCC 6 cut(s) 45, 62, 1229, 3254, 4903, 5582
PspOMI GGGCCC 1 cut(s) 4901
PspPI GGNCC 9 cut(s) 777, 813, 2459, 2864, 3801, 4901, 4902, 5276, 5689
PstI CTGCAG 3 cut(s) 915, 1114, 5121
PsyI GACNNNGTC 1 cut(s) 347
RseI CAYNNNNRTG 4 cut(s) 1006, 1417, 2195, 3072
SapI GCTCTTC 2 cut(s) 544, 2438
Sau96I GGNCC 9 cut(s) 777, 813, 2459, 2864, 3801, 4901, 4902, 5276, 5689
SbfI CCTGCAGG 1 cut(s) 915
ScaI AGTACT 1 cut(s) 249
SchI GAGTC 6 cut(s) 193, 278, 956, 2249, 2494, 4735
ScrFI CCNGG 5 cut(s) 1144, 1707, 3694, 3796, 3805
SdaI CCTGCAGG 1 cut(s) 915
SduI GDGCHC 5 cut(s) 895, 2594, 4905, 5552, 5585
SfuI TTCGAA 2 cut(s) 2796, 4948
SinI GGWCC 6 cut(s) 777, 813, 2459, 2864, 3801, 5276
SmiMI CAYNNNNRTG 4 cut(s) 1006, 1417, 2195, 3072
SmlI CTYRAG 4 cut(s) 2255, 3142, 4301, 5195
SmoI CTYRAG 4 cut(s) 2255, 3142, 4301, 5195
SnaBI TACGTA 1 cut(s) 2027
SphI GCATGC 1 cut(s) 376
Sse8387I CCTGCAGG 1 cut(s) 915
SsiI CCGC 7 cut(s) 136, 161, 378, 1711, 2034, 4850, 5355
SspI AATATT 5 cut(s) 1315, 1906, 2478, 3933, 4543
SspMI CTAG 4 cut(s) 558, 2327, 3510, 3828
StyD4I CCNGG 5 cut(s) 1142, 1705, 3692, 3794, 3803
StyI CCWWGG 8 cut(s) 312, 2209, 2934, 2973, 3057, 3384, 3552, 4701
TaaI ACNGT 7 cut(s) 2704, 2812, 2835, 2902, 4408, 4815, 4862
TaiI ACGT 4 cut(s) 1421, 2029, 2136, 3370
TatI WGTACW 7 cut(s) 247, 1839, 2835, 2897, 4279, 4815, 5657
TauI GCSGC 2 cut(s) 164, 381
TseFI GTSAC 7 cut(s) 349, 1789, 2137, 2186, 3010, 4789, 5106
Tsp45I GTSAC 7 cut(s) 349, 1789, 2137, 2186, 3010, 4789, 5106
TspGWI ACGGA 6 cut(s) 715, 1393, 2498, 3045, 3906, 4671
Tth111I GACNNNGTC 1 cut(s) 347
Vha464I CTTAAG 1 cut(s) 5195
VpaK11BI GGWCC 6 cut(s) 777, 813, 2459, 2864, 3801, 5276
VspI ATTAAT 1 cut(s) 3935
XbaI TCTAGA 1 cut(s) 2326
XceI RCATGY 8 cut(s) 376, 410, 1846, 2541, 3071, 3277, 4459, 4877
XmiI GTMKAC 4 cut(s) 366, 862, 2052, 3027
XmnI GAANNNNTTC 2 cut(s) 3783, 4595
XspI CTAG 4 cut(s) 558, 2327, 3510, 3828
ZrmI AGTACT 1 cut(s) 249
Zsp2I ATGCAT 4 cut(s) 1003, 1287, 3816, 3909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.