Rw6G017430

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000085
Physical Location & Seq
Forward (+)
34558107 .. 34561652
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017430.1

Sequence Viewer

Length: 3330 bp
ATGTCGGAGGATAATCAAGAACCGGTTGATGTTGATACCTCTGCATCTCTTCCTAGGTGGAAGCATGATGTGTTTTTGAGCTTCAGGGGTGCAGACACTCGTAAGGGTATTACAACATTTTTAAACGATCGACTGCAAAACAGAGGAATCAAAACATTCATGGATGACCAAGACCTTGAAGTAGGGGATGTTATTTCTCCCGCTCTCCTAACGGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCAAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGAAACATTTGTGAATGCATGGAAGTGGAAGAAGACAACAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGACATCTGAAGAGGAGTTTCGGAGATGCTTTTTCTGAGCATGAAAAATCAGGGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGATTTTAAAAAAGTGGCAAATTTATCTGGATGGCATACACTGAAATATAAAACTGAAAGAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACAACCTACTGGACTTGAGTTTAAATTGCCCATCGGAGGTTTTGAAGCATTTGCAGCAACAAAGCAAGCCATTGATAAGGTCATGAATGCGCTAAAAGATGATAAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTTGGGAAGACAGCAATGGTGAAATATGTTGGAGCACAAGCCCACAAAAGTAGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCAACTTGATGAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCATTAAATTGAAGGAGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGCATAGGAATTCCGAGCCACAACGAACTCCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAAAAGGGTGTTTGCCGTGACATGGATTGCCATCCAAACATACATCTCAATATCTTATCAGAAGAAGATTCTTGGAGCTTATTTGTGAAGGCAGCAAGGAAGGATTTTGACCAACCATCCAATCTCTATGATGTAGCACGTAAGGTGGCTAGAGAATGCGCTGGTCTACCAATTGCTTTGATAGCAGTTGCGAGGGCACTTCGAGATGAAGGTTTGGATGGATGGAAAGTAGCTGCTCAACGACTAAAAGAGTCCCAACCTGCCAACCCTGAAGATGAGTCAGATGTGTTTGCATGCATAAAGTTAAGCTATGATTACTTGAAATCTAATGATTCCAAATCATGCTTCTTGCTTTGCTGCTTGTTCCCAGAAGATTATGATATCCCAATTGAATACTTGTTCAAGTTTGGAATTTGTAACGGAATGTTTCAAGATTCAGACTTGCTAAAAGCCCGAGAAACTACATATCTAGTGGTGAAGGCCCTTAAAGATTCTAACTTGCTTTTGGATGCTAGAGATGACAGATATGTAAGGATGCATGATGTCATTCGGGATATGGCAATTTCGTTTTCTGAAGGTTGGTTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACGGATTGATGCACATAAAGGCTACTATGGAATCTCACTAATGGGGAACAAGATTTGCAAGCTGCCCCAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAACACAATGCTTCTCTAAATGTGATCCCAAAATCTTTTCTCCAAAGTCAGAATGAATTAAGAGTCTTGGATCTTAGCTACACTGGTATTTCATTACTACCCCAATCAATCAGTTTCTTAACCAACCTTCAAGCTTTGTATTTAGATCACTGCTGGAACATAACTGACATTTCTGTAACCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGCTCTGAAATTGTCAAGAGAAATAGGACAGTTGACCAATCTAAGGATGTTGGATGTCAGTCAAGAATATAATCCACTGACAGGGAGATGTGAAGGAAGTATTGCCACAATTCCATCTGAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCGGTGTCGATTTTGGGACTGGGGGAGTAAAATTAATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCCGGTTTATCATATTTAAGAATTTTGGAAGTTTGCATATCAGATGCAAATTGCATCCCTAAAAGTGTTGAGGTTGAACCGAATTGGGTTTACTTCGATATTAGGATTTGCAGCGACAGTGATGAACAAGGGTACACTATGAGGTTTTTTGATATTGGTTCTGGTCATTATTGTAGATCCTTGCGTCTTGAAGGTACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAATGCTGTGGTAAAGAATACTGAGACGTTGTATTATCAAAAATGCAAAGGGTTAAGTAACATTCTTGTGGAATATGCCCGTGGGAGGTTACATGGACTGAAAACTCTCTATGTAAGAGGTCGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATATGCCTGTGTTTGAGAACTTGGAAAAGTTGTATCTACGTGACCTGGATGACCTGAAGCAGTTATGTGTTGGTCAGTTACCACCTGGATCTCTCTGCAGTTTGAAAGTTTTGAAAGTCATGAGGTGTACTAACTTGGGGAATGTACTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGGAAAAACTACACTGCAGTGATGGTACTAAAGTGGAATATGTGTTTGGATGTGAAGGCTTTGAGCCGGATCAAACAAATCTGAGAAAGATGGATTTATGTAATCTAGACGCAGTAAGAAGCATATGTAATAGTCCTGCTCCACGTGGAATGTTCCAGACACTAAACGATTTGGTCATTGAATATTGCAACTTACAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAACAACTACATGTATCCGAATGCCGTTTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAATCATTGAACAACACGAAGACCGTTCTTCCAAATTTGAAGAAATTACGGTTAGAATATCTTCCCATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAATCTTGTGTATCAAGGACTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAAGCAAGACCTTTTCAATGATCCTCTTTTTTTTTTTTTTTGGAAAGAGTAG

Protein Analysis

1109

Amino Acids

126.44

Weight (kDa)

5.95

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 192 3.5e-48 TIR domain
TIR_2 PF13676 24 - 122 1.7e-16 TIR domain
NB-ARC PF00931 210 - 374 4.8e-29 NB-ARC domain
LRR_8 PF13855 577 - 635 9.7e-08 Leucine rich repeat
LRR_14 PF23598 602 - 723 4.4e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 878 - 1021 7.8e-24 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 976 - 1085 2.4e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2438
Acc36I ACCTGC 1 cut(s) 1294
AccB7I CCANNNNNTGG 1 cut(s) 1720
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 2 cut(s) 690, 1192
AccIII TCCGGA 1 cut(s) 2428
AciI CCGC 2 cut(s) 201, 2134
AcoI YGGCCR 1 cut(s) 1643
AcsI RAATTY 7 cut(s) 336, 484, 813, 969, 1437, 2229, 3127
AcuI CTGAAG 6 cut(s) 67, 404, 1317, 1620, 2112, 2687
AcvI CACGTG 1 cut(s) 2939
AdeI CACNNNGTG 1 cut(s) 276
AfaI GTAC 8 cut(s) 375, 2129, 2342, 2404, 2740, 2757, 2821, 3167
AfiI CCNNNNNNNGG 7 cut(s) 602, 1048, 1720, 2016, 2054, 2526, 3067
AflIII ACRYGT 2 cut(s) 2600, 3046
AgeI ACCGGT 1 cut(s) 22
AjnI CCWGG 2 cut(s) 2655, 2695
AjuI GAANNNNNNNTTGG 6 cut(s) 1355, 1387, 2175, 2207, 2823, 2855
AleI CACNNNNGTG 1 cut(s) 2811
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 2 cut(s) 815, 2459
Ama87I CYCGRG 1 cut(s) 1479
Aor13HI TCCGGA 1 cut(s) 2428
AoxI GGCC 3 cut(s) 672, 1506, 1643
ApeKI GCWGC 7 cut(s) 451, 620, 1118, 1259, 1383, 1708, 2319
ApoI RAATTY 7 cut(s) 336, 484, 813, 969, 1437, 2229, 3127
AseI ATTAAT 1 cut(s) 2166
AsiGI ACCGGT 1 cut(s) 22
Asp700I GAANNNNTTC 1 cut(s) 3153
AspA2I CCTAGG 1 cut(s) 53
AspLEI GCGC 2 cut(s) 658, 1187
AspS9I GGNCC 1 cut(s) 1507
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 3 cut(s) 736, 1513, 1627
AvaI CYCGRG 1 cut(s) 1479
AvrII CCTAGG 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 1225
BaeI ACNNNNGTAYC 2 cut(s) 2811, 2844
BalI TGGCCA 1 cut(s) 1645
BamHI GGATCC 2 cut(s) 361, 915
BbrPI CACGTG 1 cut(s) 2939
BbsI GAAGAC 4 cut(s) 330, 719, 866, 3119
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 7 cut(s) 463, 632, 1130, 1246, 1370, 1695, 2331
BceAI ACGGC 3 cut(s) 228, 1026, 3045
BciT130I CCWGG 2 cut(s) 2657, 2697
BciVI GTATCC 2 cut(s) 799, 3061
BcnI CCSGG 1 cut(s) 913
BcoDI GTCTC 2 cut(s) 815, 2459
BfaI CTAG 5 cut(s) 54, 1176, 1496, 1539, 2900
BfmI CTRYAG 2 cut(s) 2707, 2809
BfuAI ACCTGC 1 cut(s) 1294
BfuI GTATCC 2 cut(s) 799, 3061
BisI GCNGC 7 cut(s) 452, 621, 1119, 1260, 1384, 1709, 2320
BlnI CCTAGG 1 cut(s) 53
BlsI GCNGC 7 cut(s) 453, 622, 1120, 1261, 1385, 1710, 2321
Bme1390I CCNGG 3 cut(s) 913, 2657, 2697
BmeT110I CYCGRG 1 cut(s) 1479
BmgT120I GGNCC 1 cut(s) 1507
BmiI GGNNCC 4 cut(s) 363, 910, 917, 2422
BmrFI CCNGG 3 cut(s) 913, 2657, 2697
BmrI ACTGGG 1 cut(s) 2161
BmsI GCATC 9 cut(s) 53, 391, 526, 986, 1525, 1551, 1645, 2242, 2271
BmuI ACTGGG 1 cut(s) 2161
BpiI GAAGAC 4 cut(s) 330, 719, 866, 3119
BpmI CTGGAG 1 cut(s) 1721
BpuEI CTTGAG 2 cut(s) 602, 1970
BpuMI CCSGG 1 cut(s) 913
BsaAI YACGTR 3 cut(s) 1166, 2651, 2939
BsaBI GATNNNNATC 1 cut(s) 1056
BsaJI CCNNGG 3 cut(s) 53, 1646, 2521
BsaWI WCCGGW 3 cut(s) 22, 1931, 2428
Bsc4I CCNNNNNNNGG 7 cut(s) 602, 1048, 1720, 2016, 2054, 2526, 3067
Bse118I RCCGGY 2 cut(s) 22, 2210
Bse1I ACTGG 4 cut(s) 580, 1840, 2156, 2438
Bse3DI GCAATG 1 cut(s) 726
Bse8I GATNNNNATC 1 cut(s) 1056
BseAI TCCGGA 1 cut(s) 2428
BseBI CCWGG 2 cut(s) 2657, 2697
BseDI CCNNGG 3 cut(s) 53, 1646, 2521
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 7 cut(s) 602, 1048, 1720, 2016, 2054, 2526, 3067
BseMI GCAATG 1 cut(s) 726
BseMII CTCAG 4 cut(s) 402, 2454, 2867, 3032
BseNI ACTGG 4 cut(s) 580, 1840, 2156, 2438
BseRI GAGGAG 2 cut(s) 403, 869
BseSI GKGCMC 1 cut(s) 1225
BseXI GCAGC 7 cut(s) 463, 632, 1130, 1246, 1370, 1695, 2331
BseYI CCCAGC 1 cut(s) 838
BsgI GTGCAG 2 cut(s) 111, 470
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 3 cut(s) 674, 1508, 1645
BshTI ACCGGT 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 742
BsiHKCI CYCGRG 1 cut(s) 1479
BsiSI CCGG 6 cut(s) 23, 912, 1932, 2211, 2429, 2861
BslFI GGGAC 2 cut(s) 1264, 2162
BslI CCNNNNNNNGG 7 cut(s) 602, 1048, 1720, 2016, 2054, 2526, 3067
BsmAI GTCTC 2 cut(s) 815, 2459
BsmBI CGTCTC 1 cut(s) 2459
BsmFI GGGAC 2 cut(s) 1264, 2162
BsmI GAATGC 5 cut(s) 311, 658, 1187, 2596, 3062
BsnI GGCC 3 cut(s) 674, 1508, 1645
BsoBI CYCGRG 1 cut(s) 1479
Bsp1286I GDGCHC 2 cut(s) 742, 1225
Bsp13I TCCGGA 1 cut(s) 2428
Bsp1407I TGTACA 2 cut(s) 2127, 3165
BspACI CCGC 2 cut(s) 201, 2134
BspANI GGCC 3 cut(s) 674, 1508, 1645
BspCNI CTCAG 4 cut(s) 403, 2455, 2868, 3031
BspEI TCCGGA 1 cut(s) 2428
BspHI TCATGA 2 cut(s) 648, 2730
BspLI GGNNCC 4 cut(s) 363, 910, 917, 2422
BspMAI CTGCAG 2 cut(s) 2711, 2813
BspMI ACCTGC 1 cut(s) 1294
BspQI GCTCTTC 1 cut(s) 868
BsrBI CCGCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 726
BsrFI RCCGGY 2 cut(s) 22, 2210
BsrGI TGTACA 2 cut(s) 2127, 3165
BsrI ACTGG 4 cut(s) 580, 1840, 2156, 2438
BssAI RCCGGY 2 cut(s) 22, 2210
BssECI CCNNGG 3 cut(s) 53, 1646, 2521
BssT1I CCWWGG 1 cut(s) 53
Bst2UI CCWGG 2 cut(s) 2657, 2697
Bst4CI ACNGT 6 cut(s) 2004, 2127, 2327, 2760, 3118, 3144
Bst6I CTCTTC 6 cut(s) 54, 271, 380, 868, 938, 3005
BstAUI TGTACA 2 cut(s) 2127, 3165
BstBAI YACGTR 3 cut(s) 1166, 2651, 2939
BstC8I GCNNGC 4 cut(s) 633, 1321, 1624, 1706
BstDEI CTNAG 8 cut(s) 290, 411, 1826, 1958, 2015, 2463, 2876, 3018
BstDSI CCRYGG 2 cut(s) 1646, 2521
BstHHI GCGC 2 cut(s) 658, 1187
BstMAI GTCTC 2 cut(s) 815, 2459
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 2 cut(s) 620, 1208
BstNI CCWGG 2 cut(s) 2657, 2697
BstNSI RCATGY 4 cut(s) 1323, 2134, 2604, 3050
BstSCI CCNGG 3 cut(s) 911, 2655, 2695
BstSFI CTRYAG 2 cut(s) 2707, 2809
BstSLI GKGCMC 1 cut(s) 1225
BstV1I GCAGC 7 cut(s) 463, 632, 1130, 1246, 1370, 1695, 2331
BstV2I GAAGAC 4 cut(s) 330, 719, 866, 3119
BstX2I RGATCY 5 cut(s) 361, 915, 1822, 2384, 2699
BstYI RGATCY 5 cut(s) 361, 915, 1822, 2384, 2699
BsuI GTATCC 2 cut(s) 799, 3061
BsuRI GGCC 3 cut(s) 674, 1508, 1645
BtgI CCRYGG 2 cut(s) 1646, 2521
BtsI GCAGTG 4 cut(s) 461, 1900, 2806, 2818
BtsIMutI CAGTG 9 cut(s) 461, 503, 1833, 1900, 2048, 2332, 2806, 2818, 3026
BveI ACCTGC 1 cut(s) 1294
Cac8I GCNNGC 4 cut(s) 633, 1321, 1624, 1706
CciI TCATGA 2 cut(s) 648, 2730
CfoI GCGC 2 cut(s) 658, 1187
Cfr10I RCCGGY 2 cut(s) 22, 2210
Cfr13I GGNCC 1 cut(s) 1507
CseI GACGC 2 cut(s) 2381, 2912
Csp6I GTAC 8 cut(s) 374, 2128, 2341, 2403, 2739, 2756, 2820, 3166
CspAI ACCGGT 1 cut(s) 22
CspCI CAANNNNNGTGG 2 cut(s) 664, 699
CviQI GTAC 8 cut(s) 374, 2128, 2341, 2403, 2739, 2756, 2820, 3166
DdeI CTNAG 8 cut(s) 290, 411, 1826, 1958, 2015, 2463, 2876, 3018
DraI TTTAAA 4 cut(s) 123, 472, 589, 847
DraIII CACNNNGTG 1 cut(s) 276
DrdI GACNNNNNNGTC 1 cut(s) 2438
DseDI GACNNNNNNGTC 1 cut(s) 2438
EaeI YGGCCR 1 cut(s) 1643
Eam1104I CTCTTC 6 cut(s) 54, 271, 380, 868, 938, 3005
EarI CTCTTC 6 cut(s) 54, 271, 380, 868, 938, 3005
Eco130I CCWWGG 1 cut(s) 53
Eco32I GATATC 2 cut(s) 1408, 2100
Eco57I CTGAAG 6 cut(s) 67, 404, 1317, 1620, 2112, 2687
Eco72I CACGTG 1 cut(s) 2939
Eco88I CYCGRG 1 cut(s) 1479
EcoO109I RGGNCCY 1 cut(s) 1507
EcoRI GAATTC 2 cut(s) 336, 969
EcoRII CCWGG 2 cut(s) 2655, 2695
EcoRV GATATC 2 cut(s) 1408, 2100
EcoT14I CCWWGG 1 cut(s) 53
EcoT22I ATGCAT 3 cut(s) 311, 1325, 1566
ErhI CCWWGG 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 2459
FaqI GGGAC 2 cut(s) 1264, 2162
FauI CCCGC 1 cut(s) 208
FauNDI CATATG 1 cut(s) 2918
FblI GTMKAC 2 cut(s) 690, 1192
Fnu4HI GCNGC 7 cut(s) 452, 621, 1119, 1260, 1384, 1709, 2320
Fsp4HI GCNGC 7 cut(s) 452, 621, 1119, 1260, 1384, 1709, 2320
FspBI CTAG 5 cut(s) 54, 1176, 1496, 1539, 2900
GlaI GCGC 2 cut(s) 657, 1186
GluI GCNGC 7 cut(s) 452, 621, 1119, 1260, 1384, 1709, 2320
GsaI CCCAGC 1 cut(s) 842
GsuI CTGGAG 1 cut(s) 1721
HaeIII GGCC 3 cut(s) 674, 1508, 1645
HapII CCGG 6 cut(s) 23, 912, 1932, 2211, 2429, 2861
HgaI GACGC 2 cut(s) 2381, 2912
HhaI GCGC 2 cut(s) 658, 1187
Hin6I GCGC 2 cut(s) 656, 1185
HinP1I GCGC 2 cut(s) 656, 1185
HincII GTYRAC 1 cut(s) 2007
HindII GTYRAC 1 cut(s) 2007
HindIII AAGCTT 2 cut(s) 1884, 1946
HpaII CCGG 6 cut(s) 23, 912, 1932, 2211, 2429, 2861
HphI GGTGA 3 cut(s) 736, 1513, 1627
Hpy166II GTNNAC 6 cut(s) 691, 1193, 2007, 2299, 2343, 2739
Hpy8I GTNNAC 6 cut(s) 691, 1193, 2007, 2299, 2343, 2739
HpyCH4III ACNGT 6 cut(s) 2004, 2127, 2327, 2760, 3118, 3144
HpyCH4IV ACGT 4 cut(s) 1165, 2468, 2650, 2938
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 1208
HpyF3I CTNAG 8 cut(s) 290, 411, 1826, 1958, 2015, 2463, 2876, 3018
HpySE526I ACGT 4 cut(s) 1165, 2468, 2650, 2938
HspAI GCGC 2 cut(s) 656, 1185
Kpn2I TCCGGA 1 cut(s) 2428
LguI GCTCTTC 1 cut(s) 868
LmnI GCTCC 3 cut(s) 737, 1101, 2938
Lsp1109I GCAGC 7 cut(s) 463, 632, 1130, 1246, 1370, 1695, 2331
LweI GCATC 9 cut(s) 53, 391, 526, 986, 1525, 1551, 1645, 2242, 2271
MaeI CTAG 5 cut(s) 54, 1176, 1496, 1539, 2900
MaeII ACGT 4 cut(s) 1165, 2468, 2650, 2938
MaeIII GTNAC 7 cut(s) 1043, 1442, 1927, 2498, 2529, 2651, 2688
MbiI CCGCTC 1 cut(s) 203
MfeI CAATTG 3 cut(s) 1197, 1413, 3032
MflI RGATCY 5 cut(s) 361, 915, 1822, 2384, 2699
MhlI GDGCHC 2 cut(s) 742, 1225
MlsI TGGCCA 1 cut(s) 1645
MluNI TGGCCA 1 cut(s) 1645
MlyI GAGTC 4 cut(s) 696, 1286, 1313, 1824
MmeI TCCRAC 2 cut(s) 715, 2004
Mox20I TGGCCA 1 cut(s) 1645
Mph1103I ATGCAT 3 cut(s) 311, 1325, 1566
MroI TCCGGA 1 cut(s) 2428
MroXI GAANNNNTTC 1 cut(s) 3153
MscI TGGCCA 1 cut(s) 1645
MslI CAYNNNNRTG 3 cut(s) 314, 2507, 2811
Msp20I TGGCCA 1 cut(s) 1645
MspI CCGG 6 cut(s) 23, 912, 1932, 2211, 2429, 2861
MspR9I CCNGG 3 cut(s) 913, 2657, 2697
MunI CAATTG 3 cut(s) 1197, 1413, 3032
Mva1269I GAATGC 5 cut(s) 311, 658, 1187, 2596, 3062
MvaI CCWGG 2 cut(s) 2657, 2697
MwoI GCNNNNNNNGC 2 cut(s) 620, 1208
NciI CCSGG 1 cut(s) 913
NdeI CATATG 1 cut(s) 2918
NlaIV GGNNCC 4 cut(s) 363, 910, 917, 2422
NmuCI GTSAC 2 cut(s) 1043, 2651
NsiI ATGCAT 3 cut(s) 311, 1325, 1566
NspI RCATGY 4 cut(s) 1323, 2134, 2604, 3050
OliI CACNNNNGTG 1 cut(s) 2811
PaeI GCATGC 1 cut(s) 1323
PagI TCATGA 2 cut(s) 648, 2730
PciI ACATGT 2 cut(s) 2600, 3046
PciSI GCTCTTC 1 cut(s) 868
PctI GAATGC 5 cut(s) 311, 658, 1187, 2596, 3062
PdmI GAANNNNTTC 1 cut(s) 3153
PflMI CCANNNNNTGG 1 cut(s) 1720
PinAI ACCGGT 1 cut(s) 22
PkrI GCNGC 7 cut(s) 453, 622, 1120, 1261, 1385, 1710, 2321
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 4 cut(s) 695, 1285, 1312, 1823
PmaCI CACGTG 1 cut(s) 2939
PmlI CACGTG 1 cut(s) 2939
PpsI GAGTC 4 cut(s) 695, 1285, 1312, 1823
Ppu21I YACGTR 3 cut(s) 1166, 2651, 2939
PscI ACATGT 2 cut(s) 2600, 3046
PshBI ATTAAT 1 cut(s) 2166
Psp6I CCWGG 2 cut(s) 2655, 2695
PspCI CACGTG 1 cut(s) 2939
PspFI CCCAGC 1 cut(s) 838
PspGI CCWGG 2 cut(s) 2655, 2695
PspN4I GGNNCC 4 cut(s) 363, 910, 917, 2422
PspPI GGNCC 1 cut(s) 1507
PstI CTGCAG 2 cut(s) 2711, 2813
PsuI RGATCY 5 cut(s) 361, 915, 1822, 2384, 2699
PvuI CGATCG 1 cut(s) 130
RsaI GTAC 8 cut(s) 375, 2129, 2342, 2404, 2740, 2757, 2821, 3167
RsaNI GTAC 8 cut(s) 374, 2128, 2341, 2403, 2739, 2756, 2820, 3166
RseI CAYNNNNRTG 3 cut(s) 314, 2507, 2811
SapI GCTCTTC 1 cut(s) 868
SatI GCNGC 7 cut(s) 452, 621, 1119, 1260, 1384, 1709, 2320
Sau96I GGNCC 1 cut(s) 1507
SchI GAGTC 4 cut(s) 696, 1286, 1313, 1824
ScrFI CCNGG 3 cut(s) 913, 2657, 2697
SduI GDGCHC 2 cut(s) 742, 1225
SfaNI GCATC 9 cut(s) 53, 391, 526, 986, 1525, 1551, 1645, 2242, 2271
SfcI CTRYAG 2 cut(s) 2707, 2809
SmiMI CAYNNNNRTG 3 cut(s) 314, 2507, 2811
SmlI CTYRAG 2 cut(s) 581, 1949
SmoI CTYRAG 2 cut(s) 581, 1949
SphI GCATGC 1 cut(s) 1323
SsiI CCGC 2 cut(s) 201, 2134
SspI AATATT 2 cut(s) 2191, 2978
SspMI CTAG 5 cut(s) 54, 1176, 1496, 1539, 2900
StyD4I CCNGG 3 cut(s) 911, 2655, 2695
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 6 cut(s) 2004, 2127, 2327, 2760, 3118, 3144
TaiI ACGT 4 cut(s) 1168, 2471, 2653, 2941
TaqI TCGA 5 cut(s) 130, 1228, 2140, 2304, 3081
TatI WGTACW 4 cut(s) 2127, 2738, 2755, 3165
TscAI CASTG 9 cut(s) 461, 510, 1840, 1907, 2055, 2332, 2813, 2818, 3026
TseFI GTSAC 2 cut(s) 1043, 2651
TseI GCWGC 7 cut(s) 451, 620, 1118, 1259, 1383, 1708, 2319
Tsp45I GTSAC 2 cut(s) 1043, 2651
TspGWI ACGGA 3 cut(s) 708, 1461, 1663
TspRI CASTG 9 cut(s) 461, 510, 1840, 1907, 2055, 2332, 2813, 2818, 3026
Van91I CCANNNNNTGG 1 cut(s) 1720
VspI ATTAAT 1 cut(s) 2166
XapI RAATTY 7 cut(s) 336, 484, 813, 969, 1437, 2229, 3127
XbaI TCTAGA 1 cut(s) 2899
XceI RCATGY 4 cut(s) 1323, 2134, 2604, 3050
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XmaJI CCTAGG 1 cut(s) 53
XmiI GTMKAC 2 cut(s) 690, 1192
XmnI GAANNNNTTC 1 cut(s) 3153
XspI CTAG 5 cut(s) 54, 1176, 1496, 1539, 2900
Zsp2I ATGCAT 3 cut(s) 311, 1325, 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.