FvH4_7g31510

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000085
Physical Location & Seq
Forward (+)
22750105 .. 22758696
8592 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g31510.t1

Sequence Viewer

Length: 6648 bp
ATGGAGGGGAAAATGATGATTGCGGCAACTGTTGCATGGGTTGGTTACATGGTGATTAATAAGTGCATATGGTCTCCAATGAAGCGGGGTCCAAATCCAATAAAGAAGAAGGAAAATGATGATCCACCTTCTCTTCATTCATCATCAGTTTCTCCTCAGGAGTTGAAGAATGATCCAACATCTCTTCCTTCATCATCAGCTGAAGTATCATCATCACCTCCTCCTCCTCCTCCTGTGCTGGAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCACGGGTATTGTTTCCCATTTATGTTCTGAATTGCAACACAGTGGAATTAAAACGTTCGTGGATGAGGAAGGACTTGAACCAGGGATCAAAATTAGCTCAAATCTTGGAATGGCAATCAAAGAATCGAGGTCTGCAATCGTTCTTCTGTCACCAAACTATGCTTCTTCAATCTGGTGTTTGAATGAACTTGCGGATATTGTTCATGGCTTAGAAACCAGGAATACAATAGTTATTCCAGTTTTTTATAATGTGGAACCCCGGGATGTCCGACATCAGTTGGGGAGTTTTAAAGAAGCCTTCATCAGACATGAACAAAGGTATGGAGAGAAGGTGGAGCGGTGGAAAGCTGCTTTAACAAAAGTGGCCGAACTCTCTGGCTTGGAATTAAAAAAATATGGGTCTGATAGGCAACTTGTCGAAGGCATTGTGCAATGTGTGTGCAAGAAACTACGAGATCTGGAGATTGTGTCTACTGGAGATTTTGTAGAATTTGAAGCATCAAGGAAAGCCATGGTTAAGGTAATGAAGGCACTTAAAGATGATGGGCTCACTCCCATTGGTCTCTATGGAATGGGAGGCATTGGTAAGACAACACTGTCAAAACATGTTGCTGCCGAAGCCAAAAGAAAAGGGCTTTTTCGTCATGTGTTGATGGCTGTTGTAACACACATACCTGACTTGAGAATCATTCAAGGCACATTGGCAGATCAATTGGGCGTCAAACTCGTGGAAGAGACGGACGCTGGTAGAGCTGCTAGATTGAGTCGTGAGATATTGAGTAGAAATAAGATCCTTATAATTTTGGACGACATATGGAAGAGACTAGACTTGTCAGACATAGGGATTCCCAGCTACAAGGAGCTTCAATATTCTAGTTCCAAAGTCCTACTCACCACAAGGATATTGGAAGTTTGCGATTCCATGGGCAGCCACCCAAAAATTGCTCTTAATATCTTATCAGACAAAGATTCTTTGGACTTGTTGGTGAAGGAAACAAGAATGTCTTCAGAACAGTTGACTGTTTTCAATGATGTAGCAATGACTGTAGCAAGAGAGTGTGCTGGTCTCCCAATCGCGGTGGTAGCAATTGCGAGAGCACTTAGAGATAAAGAAATGAGTGAATGGAAAGAAGCAGCTGAACGACTTAGGTCTTCTCAACCTAGCAACCTTTATGATGTTGTGGTCAATTGCATAAAGCTAAGCTATGATCAATTGACATCTGAAGATGCGAAGTTATGCTTCTTGCTTTGCTGCCTATTTCCAGAAGACTCTGATATCCAAATTGAAGAGTTGATGGCGTATGGTATCGGGAAAGGATTGTTCGGTCGGTACGATACATTGCAAGATGCCAGAACCACTTGTCGTTCTGCGGTCAGGTCCCTTATATCTTCTAGCTTGCTTTTGGCCACTAAACATGATGGACAGGTAAGGATGCATGATGCCATCAGAGATGCTGCCATACAAATTTCTGCCCAATCTGTTTTGGTGAAAGCGGGTCGGGGTCGTCAGTTAACGACGTGGCCAAAGATTAATGCAGACAACGGATACTGTGCAATCTCATTAATGATGAACGAAATTAGAGAGCTTCCCAACAAGTTGGTATGTTCAAAACTCAAGATATTGTTATTACAACAGAACCGGTATGTCAATTCGATCCCAGAATCTTTTCTTGAATGCCTGAATGAGTTAACGGTCCTGGATCTGAGCAAAACTAGTATATCTTCACTTCCCACATCATTTTCTGTCCTAAATAACCTCCACACTTTGTATTTAGATTCTTGTGAGAAACTGTCTGACGTATCAGTAGTCGGAAAACTTAAAAAGCTTGAGATTCTTAGTCTGAGAGAATGTTGCCCTGAGGATGTTCCTGAAGAAATTGGACATTTGACCAGTCTAAGGATGTTGGACATCAGTGAATCACCGGTTGTTAAAATTGCATCTCAAGTGATATCATGTTTAAGGGGATTGGAGGAACTTTACATGCAATGTAATTTTGAGGGTTGGAGGAATGAAGTTGAGGAAGCAGGAAAAGAAACTAACCTTGGCTTTGATGAGTTAACTGGCTTGAGATATTTACACATTTTGAAGGTTGGAATACCTGATGCAACGGTCTTGCCTAAATATGTAGAAATCCCTCCAAACTGGCTGTACTTTGATATATGTATAAGCAGGGACTCTGATGTCAGAGAATCCACTTGGAAGCCACACTCCTCTCATGATCATTCAAGAACCTTGACTCTTAGCACAACAATTCATACCTTACCGGAGTGGTTTATTGATGTGGTGACAGAGAAAACAGAGAAACTACAGTACAGAAAATGCAAGGACTTGGATAATATTCTTGTGGAGTATGACCATGGGAGATTAAATGAGCTGAGGCATCTCTCTATAATTGGCCCCCATGAGAAGTTGATAGTGGTGATGAATGCAGATGCAAGGGATGCAAGGGATTCAAATAATGCTGCATTCCCGAAATTGGAAGAGTTACATCTTTTCGAGGTTGAAAACATCAACAACTTATGTGTGGGTAAGTTACCACCTTTGTCTCTATGCAAGCTCAAGTTATTGGATGTGAGAAAGTGTTATAACCTTGTGGGTGCGCTTTTACAGTCACCTTTGTTGAAGAGACTGCAATATCTGGAAAAACTTATTTGTGAACATATGTATGGCTTGGAATATGTGTTTGGCTCTGGCAAAACCACTACCTGTGAAAGGTTGCAAGAGTTGAGATTGTGCAGACTACCAGAACTCAAAAAGTTATGGAGCGGTCATGCTCCACATGAAGTCTCTCTTAATCTTAAAATTTTGGCAGTTAGTGGGTGCAAGGACCTGAAAAATCTCTTCACGTCCGATATGGCTTGTTGTCTTTCACAATTGGAAGACCTTTGGGTACATTCTTGCTTTAGCTTGGACCGAATTGTTGAAGCAAGCGAGGACATAGGGAAGAGGAATGTTATTTTTCCAAAATTGAAGAACTTAGTCTTGGAAGATCTTCCACGGCTCAGAAGGTTGTATAGTAACAGCAGTGGAGGTCCGACTTCATTGGAGATAAAGTTTCCCTCCTTGGAATACTTCTACAACTTAGAATGCCCCCGGTTCTCCCCCTCTCCTCCTAATTTGCACAGCAAGAAGCAAGTCCAAGTCAATGATATACAACATTATAGCTCTCTAAAGAAGAGGTTGTGGGAGAACATGGAACTTGAGTTTACAATTTTTGAAGATCGGCGGCGTTTGTGGAGGTATGATGTATTTTTGAGCTTCAAGGGTACTGACACTCGCAAGAGTATCGTAGTTGAACTATATAATAAACTAATTTCAAGAGGAATTAAAACATTCATGGATGATCAAGATCTTGAAGTAGGGGATGCTATTTCTCCCACTGTCCAAAAGGCAATTGAAGGATCAAGGTTTGCCATTGTTGTACTCTCTCCAAACTATGCCTCTTCTACTTGGTGTTTGGAGGAACTTGGAAGTATTTGTGAATCCATGGAAGACAACAGAGTGCTGCCAGTTTTTTACTATGTTGATCCTCTTGATGTACGACATCAGAAGGGGAGTTTCGGAGATGCTTTTACTAAATATGAAACTAGCCAACATGGGGACAAGGTGGACACGTGGAGAGATGTCTTGAAACGAGTATCCAATTTCTCTGGGTGGCATACAAAGAATCACAAGACAGAGAGAGAACTCGTGGACACAATTGTGGATTCTATGTGCAATAAAGTAGTACCTGAAGCAATTGAGTCCACAGGAGATTTCCAAGTATTCGGAGCTACAAGACAAGCTATGGATGAGGTTATGAAGGCCCTTATAGATGATATGGTCACTACCGTTGGTGTCTACGGTATGGGGGGCGTTGGCAAGACAACGATGGTAAAACATGTCGCTGCACAATCCTGCAAAAAAGGGATTTTTCACCGTGCGATTATCGCTGTTCTATCCCAAATCCCCGACTTGAGAAAAATTCAAGACACATTGGGAGATATGTTGGGCTTTAAATTTAAGGAGCATACTCAAGATGGAAGAGCTGCTAGATTACACAAGGAGATACAGAGAAGAGGAAATAGCCTTATAATCTTGGACGACGTATGGGAGAGAATAACTTTGCCAAGCATAGGTATCCCCAGCCACAGGGACCTTCAAAAGTGCAACTCCAAAGTCCTAATCACCACAAGGAGGTTGCATGTCTGTTTTGCAATGAAGTGCCAGGAAAAAATTGCTCTCCATACCTTATCAGAAGACGATTCATGGTTCTTGTTTATGAGAACGGCAAGGACGACTTTTGAATCGACCACAATCGAGAGTCTGGCAAGGAAGGTAGTAAGAGAATGTGGTGGTCTACCAATTGCATTGATATCAGTTGCAAAGGCACTTAGAGATAAAGACTTGAAAGTATGGGAAGTAGCAGTTCGAAGACTAGAGAAGTCTAAATTTGCCAACCTAGACCATGAGGACAGTGCATTCGAAATGATAAGATCAAGCTATGATTACTTGAAAGATGAAGAATGCAAGTCATTCTTCTTGCTCTGTTGTTTATTCCCAGAAGACTATGACATCCCAATAGAAGACTTGTTTAAGTATGCAATTGGGATAGGATTGTTTCGAGATGCTGAAACAATTGAAGAAGCCAGAGCATCAGCAGATTCAGTGGTCAGGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGTGAAGAAGATGGATGTGTAAAGATGCATGATGTCATTCGGGATACAGCCCTGCAAATTGCACAATCTGAAGGTGGGCGTAGGTTTCGTGTGAAAGCTGGCTGTGGTGTAAAGGGTTGGCTGACACACGGATTAGATGAAGGATGCACTGTTATTTCACTGATGCGGAACGAAATTCGCAAGCTACCAGAAGAGTTGGTATGTCCAAACCTCCAAATTTTGTTATTGAACTATAATTACGTTCTAGATAAGATCCCAGAAACCTTTTTTCAAAGTCTAAATGAATTAAGAGTCTTAGATTTTAGCTGGACGGGTATTTCAATTCTACCCCCATCATTTGGGCTTCTAACCTGCCTTCAAGCGTTGTATTTAGATGGTTGTAAGAGAATAATTGATATTTCCATGGTTGGAAAACTTATGAAGCTTGAGATTCTTAGTATGAGAGACACCAAATTAGAGGAGTTGCCGAGAGAAGTCGGAAATTTGACCAATCTGAGGATGTTGGATGTCACTGGTGGAAGAATTGCTAGGATTCCATCCAAACTGATATCAAGGTTGCAGAGTTTGGAAGAACTGTACCTAGAATGTGGATTTTGGGAGTGGGGGAGTAAGGTTGATGGAGAAGGGGATGAAATCAATGCTGGCTTTGATGAGGTAATGAGCTTGTCAAAGTTGAGACGTTTCAAGGTGTGGATCTCCAATGAAAAATGCATCCCCAAATTTGTTGACCCCGATTCATATTGGGTCGAGTTTATGCCCAAGTTTATGATATCTATTGGCAAACATGAGAAAATGGAAGCCTCTTATCAATATGGTCAAATATCCTTGACCCTGGACTGCATCAGTAACTTCCCGGACTGGTTTTTCAAGGCAGTGATGAACAAAGCAGAGACGCTAAAGTGGATTGATTGCCCACGCACTGGTTTAATCGACGTATTTGTGGAATATGAGCATGGGAGGTTATATGGACTGAAGCGTCTCATTGTAGATGGTCGACGCGATGAGGACGAGCTGATGAACACATCAACATGGGTTCCAAAAGAACCTGTGTTCGAGAACTTGGAAGAGTTGCTTCTGTGGCGCGTGGAGTTTTGTGATGTATTACCACCTGGGTCTCTATGCAATTTGAAGTTATTGGAGGTAGGATCTTGTTACAACGGGGGGGATGTACTTTTACGATCAACATTACAAGGACTACCAAATCTGGAAGAACTAAAATGTGACTCCATGGATAAGATTGAATATGTGTTTGGGTATGAAGCGTCATTGGAGGTAAAACAATCAAAATTGAGAAAAATAGAATTGTCTTATCTAAAGAGAGTAAGAAGCATTTGTGAGGGACCTGCTCCACCTGCAATGTTTCAGAATCTTCAAAGTTTGTCCATTAAGCGGTGCAAGTTGCAGGGAAGTCTCTTCACCTATGATGTTGCTCAACGTCTTTTTCAATTGAAAATCCTTGTTTTAAAGGAGTGCCCCTTACTGGAAAGGATAGTTGAAGCAAGCAACAAGAAGATCGTTCTTCCAATATTGAAGCAATTGAGAAAAACAATCCGGATAGCTAGTTTGTCGGGGACGAGAGGGAAGAGTCAAACGAGATGGAAGAGTCGAGCGAGAGGGAGGAGTCAGAGTCGGACGAGTGCAATTCTGTACCAGACTGGAGAGAGGAGCAGGCCTGTTTGA

Protein Analysis

2216

Amino Acids

251.17

Weight (kDa)

6.68

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 82 - 246 1.1e-47 TIR domain
TIR_2 PF13676 84 - 196 2.9e-15 TIR domain
NB-ARC PF00931 264 - 427 2.9e-24 NB-ARC domain
LRR_14 PF23598 631 - 803 6.7e-15 Leucine-rich repeat region
LRR_8 PF13855 631 - 688 8.5e-07 Leucine rich repeat
LRR_RPS2 PF23247 920 - 1061 9.8e-21 Plant disease resistance protein RPS2-like, leucine-rich repeats
TIR PF01582 1175 - 1339 6.7e-50 TIR domain
TIR_2 PF13676 1177 - 1268 1.8e-16 TIR domain
NB-ARC PF00931 1355 - 1518 1.4e-27 NB-ARC domain
LRR_8 PF13855 1725 - 1783 9e-08 Leucine rich repeat
LRR_14 PF23598 1778 - 1885 5.8e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 2027 - 2147 3.8e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 528, 1079, 2868, 4355
AarI CACCTGC 1 cut(s) 6328
AasI GACNNNNNNGTC 3 cut(s) 877, 2462, 5943
Acc36I ACCTGC 3 cut(s) 5327, 6319, 6328
Acc65I GGTACC 1 cut(s) 4938
AccB1I GGYRCC 1 cut(s) 4938
AccB7I CCANNNNNTGG 2 cut(s) 5306, 5888
AccBSI CCGCTC 2 cut(s) 619, 3048
AccI GTMKAC 4 cut(s) 752, 4122, 4621, 5962
AccII CGCG 3 cut(s) 1358, 5967, 6051
AccIII TCCGGA 1 cut(s) 6519
AclI AACGTT 1 cut(s) 335
AcoI YGGCCR 3 cut(s) 645, 1686, 1802
AcuI CTGAAG 7 cut(s) 222, 1272, 1524, 2172, 4035, 5058, 5960
AcvI CACGTG 1 cut(s) 3897
AcyI GRCGYC 1 cut(s) 999
AflIII ACRYGT 3 cut(s) 886, 3894, 4162
AgeI ACCGGT 2 cut(s) 1920, 2203
AhlI ACTAGT 1 cut(s) 1994
AjiI CACGTC 2 cut(s) 1800, 3129
AjnI CCWGG 6 cut(s) 361, 497, 1977, 4488, 5799, 6076
AleI CACNNNNGTG 1 cut(s) 3983
Alw21I GWGCWC 1 cut(s) 1381
Ama87I CYCGRG 1 cut(s) 540
Aor13HI TCCGGA 1 cut(s) 6519
AoxI GGCC 6 cut(s) 645, 1686, 1802, 2677, 4086, 6638
ArsI GACNNNNNNTTYG 2 cut(s) 4727, 4759
AseI ATTAAT 3 cut(s) 57, 1812, 1844
AsiGI ACCGGT 2 cut(s) 1920, 2203
Asp700I GAANNNNTTC 3 cut(s) 1285, 1947, 3273
Asp718I GGTACC 1 cut(s) 4938
AspLEI GCGC 2 cut(s) 2884, 6051
AsuC2I CCSGG 4 cut(s) 541, 542, 3376, 5822
AsuII TTCGAA 2 cut(s) 4693, 4746
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 9 cut(s) 89, 269, 1659, 1975, 3109, 3193, 3314, 4417, 6308
AxyI CCTNAGG 2 cut(s) 156, 2139
BaeGI GKGCMC 1 cut(s) 6443
BalI TGGCCA 2 cut(s) 1688, 1804
BanI GGYRCC 1 cut(s) 4938
BanII GRGCYC 1 cut(s) 831
BauI CACGAG 2 cut(s) 1007, 3971
BbrPI CACGTG 1 cut(s) 3897
BbsI GAAGAC 9 cut(s) 1278, 1425, 1554, 3168, 3780, 4527, 4702, 4833, 4854
Bbv12I GWGCWC 1 cut(s) 1381
BbvCI CCTCAGC 1 cut(s) 2657
BceAI ACGGC 2 cut(s) 3296, 4566
BcgI CGANNNNNNTGC 2 cut(s) 3550, 3584
BciT130I CCWGG 6 cut(s) 363, 499, 1979, 4490, 5801, 6078
BciVI GTATCC 4 cut(s) 1820, 3931, 4412, 5005
BclI TGATCA 3 cut(s) 1489, 2500, 3625
BcnI CCSGG 4 cut(s) 541, 542, 3376, 5822
BcuI ACTAGT 1 cut(s) 1994
BfmI CTRYAG 2 cut(s) 1326, 2588
BfuAI ACCTGC 3 cut(s) 5327, 6319, 6328
BfuI GTATCC 4 cut(s) 1820, 3931, 4412, 5005
BglII AGATCT 3 cut(s) 736, 3271, 3631
BlpI GCTNAGC 1 cut(s) 1481
Bme18I GGWCC 9 cut(s) 89, 269, 1659, 1975, 3109, 3193, 3314, 4417, 6308
BmeT110I CYCGRG 1 cut(s) 540
BmgBI CACGTC 2 cut(s) 1800, 3129
BmiI GGNNCC 9 cut(s) 90, 270, 537, 1661, 2680, 4418, 4940, 6003, 6309
BoxI GACNNNNGTC 1 cut(s) 1429
BpiI GAAGAC 9 cut(s) 1278, 1425, 1554, 3168, 3780, 4527, 4702, 4833, 4854
BpmI CTGGAG 4 cut(s) 260, 761, 777, 6645
Bpu10I CCTNAGC 1 cut(s) 2657
Bpu1102I GCTNAGC 1 cut(s) 1481
Bpu14I TTCGAA 2 cut(s) 4693, 4746
BpuMI CCSGG 4 cut(s) 541, 542, 3376, 5822
BsaAI YACGTR 1 cut(s) 3897
BsaBI GATNNNNATC 1 cut(s) 3630
BsaHI GRCGYC 1 cut(s) 999
BsaI GGTCTC 4 cut(s) 78, 848, 1352, 6087
BsaWI WCCGGW 4 cut(s) 1920, 2203, 2545, 6519
Bse118I RCCGGY 2 cut(s) 1920, 2203
Bse21I CCTNAGG 2 cut(s) 156, 2139
Bse3DI GCAATG 6 cut(s) 719, 1326, 1619, 2273, 4485, 6330
Bse8I GATNNNNATC 1 cut(s) 3630
BseAI TCCGGA 1 cut(s) 6519
BseBI CCWGG 6 cut(s) 363, 499, 1979, 4490, 5801, 6078
BseJI GATNNNNATC 1 cut(s) 3630
BseMI GCAATG 6 cut(s) 719, 1326, 1619, 2273, 4485, 6330
BseMII CTCAG 7 cut(s) 170, 1976, 2114, 2130, 2648, 3298, 5453
BseSI GKGCMC 1 cut(s) 6443
BseYI CCCAGC 2 cut(s) 1130, 4406
BsgI GTGCAG 2 cut(s) 3037, 4155
Bsh1236I CGCG 3 cut(s) 1358, 5967, 6051
Bsh1285I CGRYCG 1 cut(s) 1609
BshFI GGCC 6 cut(s) 647, 1688, 1804, 2679, 4088, 6640
BshNI GGYRCC 1 cut(s) 4938
BshTI ACCGGT 2 cut(s) 1920, 2203
BsiEI CGRYCG 1 cut(s) 1609
BsiHKAI GWGCWC 1 cut(s) 1381
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 7 cut(s) 541, 1921, 2204, 2546, 3376, 5822, 6520
BslFI GGGAC 6 cut(s) 1645, 2468, 3896, 4430, 6321, 6553
BsmBI CGTCTC 4 cut(s) 1010, 5638, 5852, 5951
BsmFI GGGAC 6 cut(s) 1645, 2468, 3896, 4430, 6321, 6553
BsmI GAATGC 6 cut(s) 1961, 2713, 2747, 3374, 4742, 4793
BsnI GGCC 6 cut(s) 647, 1688, 1804, 2679, 4088, 6640
Bso31I GGTCTC 4 cut(s) 78, 848, 1352, 6087
BsoBI CYCGRG 1 cut(s) 540
Bsp119I TTCGAA 2 cut(s) 4693, 4746
Bsp1286I GDGCHC 3 cut(s) 831, 1381, 6443
Bsp13I TCCGGA 1 cut(s) 6519
Bsp1720I GCTNAGC 1 cut(s) 1481
Bsp19I CCATGG 6 cut(s) 792, 1203, 2638, 3768, 5370, 6195
BspANI GGCC 6 cut(s) 647, 1688, 1804, 2679, 4088, 6640
BspCNI CTCAG 7 cut(s) 169, 1977, 2115, 2131, 2649, 3297, 5454
BspEI TCCGGA 1 cut(s) 6519
BspFNI CGCG 3 cut(s) 1358, 5967, 6051
BspHI TCATGA 1 cut(s) 2497
BspLI GGNNCC 9 cut(s) 90, 270, 537, 1661, 2680, 4418, 4940, 6003, 6309
BspMI ACCTGC 3 cut(s) 5327, 6319, 6328
BspQI GCTCTTC 1 cut(s) 4300
BspT104I TTCGAA 2 cut(s) 4693, 4746
BspT107I GGYRCC 1 cut(s) 4938
BspTNI GGTCTC 4 cut(s) 78, 848, 1352, 6087
BsrBI CCGCTC 2 cut(s) 619, 3048
BsrDI GCAATG 6 cut(s) 719, 1326, 1619, 2273, 4485, 6330
BsrFI RCCGGY 2 cut(s) 1920, 2203
BssAI RCCGGY 2 cut(s) 1920, 2203
BssNI GRCGYC 1 cut(s) 999
BssSI CACGAG 2 cut(s) 1007, 3971
BssT1I CCWWGG 8 cut(s) 792, 1203, 2323, 2638, 3345, 3768, 5370, 6195
Bst2BI CACGAG 2 cut(s) 1007, 3971
Bst2UI CCWGG 6 cut(s) 363, 499, 1979, 4490, 5801, 6078
BstACI GRCGYC 1 cut(s) 999
BstAPI GCANNNNNTGC 2 cut(s) 32, 2723
BstBAI YACGTR 1 cut(s) 3897
BstBI TTCGAA 2 cut(s) 4693, 4746
BstC8I GCNNGC 9 cut(s) 1679, 2837, 3211, 4958, 5068, 5150, 5611, 6469, 6638
BstDSI CCRYGG 7 cut(s) 792, 1203, 2638, 3278, 3768, 5370, 6195
BstENI CCTNNNNNAGG 1 cut(s) 2992
BstFNI CGCG 3 cut(s) 1358, 5967, 6051
BstHHI GCGC 2 cut(s) 2884, 6051
BstMCI CGRYCG 1 cut(s) 1609
BstMWI GCNNNNNNNGC 8 cut(s) 32, 899, 1031, 1364, 2723, 4211, 6046, 6320
BstNI CCWGG 6 cut(s) 363, 499, 1979, 4490, 5801, 6078
BstNSI RCATGY 4 cut(s) 890, 2266, 4166, 4469
BstPAI GACNNNNGTC 1 cut(s) 1429
BstSFI CTRYAG 2 cut(s) 1326, 2588
BstSLI GKGCMC 1 cut(s) 6443
BstUI CGCG 3 cut(s) 1358, 5967, 6051
BstV2I GAAGAC 9 cut(s) 1278, 1425, 1554, 3168, 3780, 4527, 4702, 4833, 4854
BstX2I RGATCY 8 cut(s) 736, 1071, 1981, 3271, 3631, 5220, 5661, 6113
BstXI CCANNNNNNTGG 1 cut(s) 1879
BstYI RGATCY 8 cut(s) 736, 1071, 1981, 3271, 3631, 5220, 5661, 6113
Bsu36I CCTNAGG 2 cut(s) 156, 2139
BsuI GTATCC 4 cut(s) 1820, 3931, 4412, 5005
BsuRI GGCC 6 cut(s) 647, 1688, 1804, 2679, 4088, 6640
BtgI CCRYGG 7 cut(s) 792, 1203, 2638, 3278, 3768, 5370, 6195
BtgZI GCGATG 1 cut(s) 5982
BtrI CACGTC 2 cut(s) 1800, 3129
BtsI GCAGTG 2 cut(s) 3313, 5847
BveI ACCTGC 3 cut(s) 5327, 6319, 6328
Cac8I GCNNGC 9 cut(s) 1679, 2837, 3211, 4958, 5068, 5150, 5611, 6469, 6638
CciI TCATGA 1 cut(s) 2497
CfoI GCGC 2 cut(s) 2884, 6051
Cfr10I RCCGGY 2 cut(s) 1920, 2203
Cfr9I CCCGGG 1 cut(s) 540
CseI GACGC 6 cut(s) 988, 1031, 5869, 5933, 5973, 6219
CspAI ACCGGT 2 cut(s) 1920, 2203
CspCI CAANNNNNGTGG 2 cut(s) 1341, 1376
DraI TTTAAA 3 cut(s) 571, 4279, 6432
DrdI GACNNNNNNGTC 3 cut(s) 877, 2462, 5943
DseDI GACNNNNNNGTC 3 cut(s) 877, 2462, 5943
EaeI YGGCCR 3 cut(s) 645, 1686, 1802
Eco130I CCWWGG 8 cut(s) 792, 1203, 2323, 2638, 3345, 3768, 5370, 6195
Eco147I AGGCCT 1 cut(s) 6640
Eco24I GRGCYC 1 cut(s) 831
Eco31I GGTCTC 4 cut(s) 78, 848, 1352, 6087
Eco32I GATATC 5 cut(s) 1558, 2232, 4638, 5517, 5739
Eco47I GGWCC 9 cut(s) 89, 269, 1659, 1975, 3109, 3193, 3314, 4417, 6308
Eco57I CTGAAG 7 cut(s) 222, 1272, 1524, 2172, 4035, 5058, 5960
Eco72I CACGTG 1 cut(s) 3897
Eco81I CCTNAGG 2 cut(s) 156, 2139
Eco88I CYCGRG 1 cut(s) 540
EcoNI CCTNNNNNAGG 1 cut(s) 2992
EcoO109I RGGNCCY 6 cut(s) 269, 1659, 3109, 4087, 4417, 6308
EcoRII CCWGG 6 cut(s) 361, 497, 1977, 4488, 5799, 6076
EcoRV GATATC 5 cut(s) 1558, 2232, 4638, 5517, 5739
EcoT14I CCWWGG 8 cut(s) 792, 1203, 2323, 2638, 3345, 3768, 5370, 6195
EcoT22I ATGCAT 3 cut(s) 1719, 4998, 5681
EcoT38I GRGCYC 1 cut(s) 831
ErhI CCWWGG 8 cut(s) 792, 1203, 2323, 2638, 3345, 3768, 5370, 6195
Esp3I CGTCTC 4 cut(s) 1010, 5638, 5852, 5951
FaqI GGGAC 6 cut(s) 1645, 2468, 3896, 4430, 6321, 6553
FauI CCCGC 2 cut(s) 78, 1768
FauNDI CATATG 3 cut(s) 68, 1094, 2943
FbaI TGATCA 3 cut(s) 1489, 2500, 3625
FblI GTMKAC 4 cut(s) 752, 4122, 4621, 5962
FriOI GRGCYC 1 cut(s) 831
GlaI GCGC 2 cut(s) 2883, 6050
GsaI CCCAGC 2 cut(s) 1134, 4410
GsuI CTGGAG 4 cut(s) 260, 761, 777, 6645
HaeIII GGCC 6 cut(s) 647, 1688, 1804, 2679, 4088, 6640
HapII CCGG 7 cut(s) 541, 1921, 2204, 2546, 3376, 5822, 6520
HgaI GACGC 6 cut(s) 988, 1031, 5869, 5933, 5973, 6219
HhaI GCGC 2 cut(s) 2884, 6051
Hin1I GRCGYC 1 cut(s) 999
Hin6I GCGC 2 cut(s) 2882, 6049
HinP1I GCGC 2 cut(s) 2882, 6049
HincII GTYRAC 6 cut(s) 1299, 1794, 1971, 2340, 5695, 5963
HindII GTYRAC 6 cut(s) 1299, 1794, 1971, 2340, 5695, 5963
HindIII AAGCTT 2 cut(s) 2105, 5390
HpaI GTTAAC 3 cut(s) 1794, 1971, 2340
HpaII CCGG 7 cut(s) 541, 1921, 2204, 2546, 3376, 5822, 6520
Hpy99I CGWCG 4 cut(s) 1801, 4370, 5903, 5967
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 899, 1031, 1364, 2723, 4211, 6046, 6320
Hsp92I GRCGYC 1 cut(s) 999
HspAI GCGC 2 cut(s) 2882, 6049
Kpn2I TCCGGA 1 cut(s) 6519
KpnI GGTACC 1 cut(s) 4942
Ksp22I TGATCA 3 cut(s) 1489, 2500, 3625
KspAI GTTAAC 3 cut(s) 1794, 1971, 2340
LguI GCTCTTC 1 cut(s) 4300
LmnI GCTCC 8 cut(s) 616, 1141, 3045, 3061, 4052, 4288, 6319, 6633
MbiI CCGCTC 2 cut(s) 619, 3048
MflI RGATCY 8 cut(s) 736, 1071, 1981, 3271, 3631, 5220, 5661, 6113
MhlI GDGCHC 3 cut(s) 831, 1381, 6443
MlsI TGGCCA 2 cut(s) 1688, 1804
MluNI TGGCCA 2 cut(s) 1688, 1804
Mox20I TGGCCA 2 cut(s) 1688, 1804
Mph1103I ATGCAT 3 cut(s) 1719, 4998, 5681
MroI TCCGGA 1 cut(s) 6519
MroXI GAANNNNTTC 3 cut(s) 1285, 1947, 3273
MscI TGGCCA 2 cut(s) 1688, 1804
MslI CAYNNNNRTG 3 cut(s) 2946, 3983, 5995
Msp20I TGGCCA 2 cut(s) 1688, 1804
MspA1I CMGCKG 2 cut(s) 200, 1418
MspI CCGG 7 cut(s) 541, 1921, 2204, 2546, 3376, 5822, 6520
Mva1269I GAATGC 6 cut(s) 1961, 2713, 2747, 3374, 4742, 4793
MvaI CCWGG 6 cut(s) 363, 499, 1979, 4490, 5801, 6078
MvnI CGCG 3 cut(s) 1358, 5967, 6051
MwoI GCNNNNNNNGC 8 cut(s) 32, 899, 1031, 1364, 2723, 4211, 6046, 6320
NciI CCSGG 4 cut(s) 541, 542, 3376, 5822
NcoI CCATGG 6 cut(s) 792, 1203, 2638, 3768, 5370, 6195
NdeI CATATG 3 cut(s) 68, 1094, 2943
NlaIV GGNNCC 9 cut(s) 90, 270, 537, 1661, 2680, 4418, 4940, 6003, 6309
NmeAIII GCCGAG 1 cut(s) 5460
NmuCI GTSAC 6 cut(s) 429, 2566, 2892, 4105, 5476, 6188
NsiI ATGCAT 3 cut(s) 1719, 4998, 5681
NspI RCATGY 4 cut(s) 890, 2266, 4166, 4469
NspV TTCGAA 2 cut(s) 4693, 4746
OliI CACNNNNGTG 1 cut(s) 3983
PagI TCATGA 1 cut(s) 2497
PaqCI CACCTGC 1 cut(s) 6328
PceI AGGCCT 1 cut(s) 6640
PciI ACATGT 2 cut(s) 886, 4162
PciSI GCTCTTC 1 cut(s) 4300
PcsI WCGNNNNNNNCGW 2 cut(s) 1611, 4556
PctI GAATGC 6 cut(s) 1961, 2713, 2747, 3374, 4742, 4793
PdmI GAANNNNTTC 3 cut(s) 1285, 1947, 3273
PflMI CCANNNNNTGG 2 cut(s) 5306, 5888
PfoI TCCNGGA 2 cut(s) 1977, 5820
PinAI ACCGGT 2 cut(s) 1920, 2203
PmaCI CACGTG 1 cut(s) 3897
PmlI CACGTG 1 cut(s) 3897
Ppu21I YACGTR 1 cut(s) 3897
PpuMI RGGWCCY 5 cut(s) 269, 1659, 3109, 4417, 6308
PscI ACATGT 2 cut(s) 886, 4162
PshAI GACNNNNGTC 1 cut(s) 1429
PshBI ATTAAT 3 cut(s) 57, 1812, 1844
PsiI TTATAA 4 cut(s) 528, 1079, 2868, 4355
Psp1406I AACGTT 1 cut(s) 335
Psp5II RGGWCCY 5 cut(s) 269, 1659, 3109, 4417, 6308
Psp6I CCWGG 6 cut(s) 361, 497, 1977, 4488, 5799, 6076
PspCI CACGTG 1 cut(s) 3897
PspFI CCCAGC 2 cut(s) 1130, 4406
PspGI CCWGG 6 cut(s) 361, 497, 1977, 4488, 5799, 6076
PspN4I GGNNCC 9 cut(s) 90, 270, 537, 1661, 2680, 4418, 4940, 6003, 6309
PspPPI RGGWCCY 5 cut(s) 269, 1659, 3109, 4417, 6308
PsrI GAACNNNNNNTAC 2 cut(s) 5189, 5221
PsuI RGATCY 8 cut(s) 736, 1071, 1981, 3271, 3631, 5220, 5661, 6113
PvuII CAGCTG 2 cut(s) 200, 1418
RseI CAYNNNNRTG 3 cut(s) 2946, 3983, 5995
SalI GTCGAC 1 cut(s) 5961
SapI GCTCTTC 1 cut(s) 4300
SduI GDGCHC 3 cut(s) 831, 1381, 6443
SfcI CTRYAG 2 cut(s) 1326, 2588
SfuI TTCGAA 2 cut(s) 4693, 4746
SinI GGWCC 9 cut(s) 89, 269, 1659, 1975, 3109, 3193, 3314, 4417, 6308
SmaI CCCGGG 1 cut(s) 542
SmiMI CAYNNNNRTG 3 cut(s) 2946, 3983, 5995
SpeI ACTAGT 1 cut(s) 1994
SseBI AGGCCT 1 cut(s) 6640
SspI AATATT 3 cut(s) 1151, 2620, 6495
StuI AGGCCT 1 cut(s) 6640
StyI CCWWGG 8 cut(s) 792, 1203, 2323, 2638, 3345, 3768, 5370, 6195
TaqII GACCGA 2 cut(s) 1595, 3210
TatI WGTACW 4 cut(s) 2430, 2592, 3703, 6136
TauI GCSGC 2 cut(s) 26, 3511
TseFI GTSAC 6 cut(s) 429, 2566, 2892, 4105, 5476, 6188
Tsp45I GTSAC 6 cut(s) 429, 2566, 2892, 4105, 5476, 6188
TspGWI ACGGA 3 cut(s) 1034, 1839, 5112
TspMI CCCGGG 1 cut(s) 540
Van91I CCANNNNNTGG 2 cut(s) 5306, 5888
VpaK11BI GGWCC 9 cut(s) 89, 269, 1659, 1975, 3109, 3193, 3314, 4417, 6308
VspI ATTAAT 3 cut(s) 57, 1812, 1844
XagI CCTNNNNNAGG 1 cut(s) 2992
XbaI TCTAGA 1 cut(s) 5212
XceI RCATGY 4 cut(s) 890, 2266, 4166, 4469
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmaI CCCGGG 1 cut(s) 540
XmiI GTMKAC 4 cut(s) 752, 4122, 4621, 5962
XmnI GAANNNNTTC 3 cut(s) 1285, 1947, 3273
Zsp2I ATGCAT 3 cut(s) 1719, 4998, 5681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.