Rw5G039500

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69691084 .. 69699824
8741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039500.1

Sequence Viewer

Length: 2121 bp
ATGAATATTTGCTATTCCATGGAGTGCCAAGCAAGAATTCATCTCGATACCTTATCAGAAGAAGATTCTTGGAACCTTTTTGTGAAGAAATCGGGAAAGTCTTTTCATACATCTATCCGGCTGTATGGTCCAGCAAGGATGTTAGCTAGAGAATGTGGTGGTTTCCCACTTGTACTGATAACAGTTGCTCGGGCACTTGGAGATAAACACATAGACGACTGGAAAGAAGCAGCTCGACGAGTACGCACATCCCAACTAGCCAGTCCTGAGGATGGGGGAGATCCATTCAGATTTATAAAGTTGAGCTATGATTACTTGAGGTCTGATGATGCCAGATCATTCTTCTTGCTTAGCTGCCTATTCCCAGAAGACTATGACATCCCAATCGAAGACTTGCTCAAGTATGCAATCGGGAAAGGATTGCTTCAAGATGTCAAAACAATCCAAGAAGCCAGAGCTACAGCAAATTCGGTGATCAAGTCCCTCGGAACTTCCGGCTTACTTTTGGACTGTACAAAGGAAGGATGTGTAAGGATGCATGATGTAGTGCGGGATGTAGCCGTGTTGATTTTGTTGTCTGAAGATGGTCATAGATCTTTGGTAAAATCGGGTTGTGCATTACGGCATTGGCCAAGGATTGGTCTGCAGAGAGGCTACTCTGCAATCTCACTAATGATGAATGATATTGACAGGCTTCCTGAAGATTTGGTATGCTCAGAACTCCAGATTTTGTTACTACAAAAAAATGCTGGTATATATGAGATCCCAGAAACCTTTTTTCAAAGTTCGAATGCACTAAGGGTCTTAGATATTAACTTTACTAATATTTCGTCACCACCCAGGTCAATTAATATCCTAACCAATCTCCAAGCTTTGTTTTTAGATTATTGCAATAAGTTAATTGACATATCCATATTCAGAAACTTGAAGAAACTCAAGATTCTTAGTATGAAGGGGTTTCCTCTTCAGGAGTTACCAAAAGAGATAGGATACTTGACCAATCTAAGGATGCTGGACTTAAGTGGGAGTTACACTCTTGGTATATTTCCATATAATGTCATGTCAAGATTGTGTAACTTAGAAGAACTGTACATGCAATGTAATTCTGCGGGCTGGGGAAGCAAAATTCATGGAGGTGGAGAAGAAACTAATGTTGATCCTGATGAGTTGATTGGCTTGACACATTTAAACATTGTGAAGCTTCACATATCTGATGCAGAATGCTTGCCTAAAACTGTTATACGCAGTTTGAGTTGGACCTGTGTCCATTTTGATTTCCCATCAGTTATTGAACCAAACAAGGAAGCAGTGAACAAGAAGATAGTGTTTCCAGAACTGAAGATCTTGGCTTTGTCATATCTTCCACATCTCACAAGGTTCTGCAGTACTGAAAGTGCTACTATTGAGTGCCCTTTATTGGAGCACTTGCATGTGCAGGACTGCCCGCATTTTGCAACCTCTATTTCTGACTTCCACAGCTGGAAGCAATTACAAGTTATTGATCTACGACATTTGAGAATGAGCAAAGACATGGTCAACATCAATTCAGCTGGCCAGCACACCGTGGACATCCTTGAGAGCCTACCCCAGAATGTCAAAACGCGGGCTGAGGTTATTAGAGAAATTCAGCGTCAATATGATGAGTTACAGACCAAGTTCTTCAAGGAGAGAGCAGCACTTGAAGCTAAAAAGAGATACATGATTGTAAATGGTGTTGTTGAAGCTGATGGAGTTACTACTGAAGCAGCAGCAAATCAAGAGGACAAAGCTGCAAAAAAGAGAGGAGTGTGTGATTTCTCCCTCAATGCACTAAAGAATGATGACAAAGCGGTAGGTACAGAGATTAAATGGTATCTGAAAGAATGTCTGACACAGAGGATCCTTAAGGAGAAGTCTAAGAAGGGATCGAAGATATCTAAGCCACTCACTAGAACTGAAACTTGTGAAAGTTTCTTCAACTTCCTTTTTCCACCTCAAGTCCCCAAGGATGATGCAGATATTGATGAAGGTGTTGCTGAGGAACTCAAGAACCTGAAGAAACAAGAATATGATATTGGGTCAACCATTCGATATAGGATTACCCCACATGCTGTTTCATGGTTCATGGGTGAAGCTTCTTAG

Protein Analysis

706

Amino Acids

79.97

Weight (kDa)

6.44

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 240 - 298 1e-05 Leucine rich repeat
LRR_14 PF23598 294 - 404 4.4e-06 Leucine-rich repeat region
NAP PF00956 609 - 704 1.2e-21 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 296
AccB7I CCANNNNNTGG 1 cut(s) 638
AccII CGCG 1 cut(s) 1603
AciI CCGC 5 cut(s) 550, 1109, 1445, 1603, 1829
AclWI GGATC 6 cut(s) 275, 757, 1151, 1873, 1886, 1912
AcoI YGGCCR 2 cut(s) 629, 1552
AcsI RAATTY 4 cut(s) 36, 466, 1125, 1623
AcuI CTGAAG 6 cut(s) 600, 720, 950, 1358, 1761, 2054
AdeI CACNNNGTG 1 cut(s) 1564
AfaI GTAC 6 cut(s) 174, 243, 514, 1091, 1387, 1837
AfiI CCNNNNNNNGG 4 cut(s) 272, 638, 1005, 1417
AflII CTTAAG 2 cut(s) 1018, 1883
AgsI TTSAA 8 cut(s) 428, 782, 928, 1292, 1663, 1682, 1721, 1957
AjnI CCWGG 1 cut(s) 839
AjuI GAANNNNNNNTTGG 4 cut(s) 21, 53, 2037, 2069
Alw21I GWGCWC 1 cut(s) 1425
AlwI GGATC 6 cut(s) 275, 757, 1151, 1873, 1886, 1912
Ama87I CYCGRG 1 cut(s) 189
AoxI GGCC 2 cut(s) 629, 1552
ApeKI GCWGC 6 cut(s) 230, 354, 1673, 1745, 1748, 1769
ApoI RAATTY 4 cut(s) 36, 466, 1125, 1623
ArsI GACNNNNNNTTYG 2 cut(s) 1518, 1550
AseI ATTAAT 1 cut(s) 849
AspS9I GGNCC 2 cut(s) 128, 1257
AsuHPI GGTGA 3 cut(s) 484, 825, 2120
AsuII TTCGAA 1 cut(s) 788
AvaI CYCGRG 1 cut(s) 189
AvaII GGWCC 2 cut(s) 128, 1257
AxyI CCTNAGG 1 cut(s) 267
BaeGI GKGCMC 2 cut(s) 196, 1412
BalI TGGCCA 2 cut(s) 631, 1554
BamHI GGATCC 1 cut(s) 1878
BbsI GAAGAC 2 cut(s) 375, 396
Bbv12I GWGCWC 1 cut(s) 1425
BbvCI CCTCAGC 2 cut(s) 1608, 2016
BbvI GCAGC 6 cut(s) 242, 341, 1685, 1756, 1757, 1760
BccI CCATC 4 cut(s) 266, 578, 1288, 1721
BceAI ACGGC 2 cut(s) 545, 638
BciT130I CCWGG 1 cut(s) 841
BciVI GTATCC 1 cut(s) 983
BclI TGATCA 1 cut(s) 474
BfaI CTAG 3 cut(s) 147, 257, 1929
BfmI CTRYAG 3 cut(s) 459, 644, 1381
BfrI CTTAAG 2 cut(s) 1018, 1883
BfuI GTATCC 1 cut(s) 983
BglII AGATCT 2 cut(s) 593, 1341
BisI GCNGC 6 cut(s) 231, 355, 1674, 1746, 1749, 1770
BlpI GCTNAGC 1 cut(s) 350
BlsI GCNGC 6 cut(s) 232, 356, 1675, 1747, 1750, 1771
BmcAI AGTACT 1 cut(s) 1387
Bme1390I CCNGG 1 cut(s) 841
Bme18I GGWCC 2 cut(s) 128, 1257
BmeT110I CYCGRG 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 128, 1257
BmiI GGNNCC 2 cut(s) 74, 1880
BmrFI CCNGG 1 cut(s) 841
BmsI GCATC 5 cut(s) 319, 525, 999, 1204, 1981
BoxI GACNNNNGTC 1 cut(s) 1262
BpiI GAAGAC 2 cut(s) 375, 396
BplI GAGNNNNNCTC 2 cut(s) 1018, 1050
BpmI CTGGAG 1 cut(s) 707
Bpu10I CCTNAGC 2 cut(s) 1608, 2016
Bpu1102I GCTNAGC 1 cut(s) 350
Bpu14I TTCGAA 1 cut(s) 788
BpuEI CTTGAG 6 cut(s) 337, 383, 920, 1595, 1959, 2009
BsaJI CCNNGG 6 cut(s) 18, 484, 632, 839, 1563, 1983
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 638, 1005, 1417
Bse1I ACTGG 2 cut(s) 224, 261
Bse21I CCTNAGG 1 cut(s) 267
Bse3DI GCAATG 1 cut(s) 1103
BseBI CCWGG 1 cut(s) 841
BseDI CCNNGG 6 cut(s) 18, 484, 632, 839, 1563, 1983
BseLI CCNNNNNNNGG 4 cut(s) 272, 638, 1005, 1417
BseMI GCAATG 1 cut(s) 1103
BseMII CTCAG 4 cut(s) 258, 729, 1599, 2007
BseNI ACTGG 2 cut(s) 224, 261
BseRI GAGGAG 1 cut(s) 1797
BseSI GKGCMC 2 cut(s) 196, 1412
BseXI GCAGC 6 cut(s) 242, 341, 1685, 1756, 1757, 1760
BseYI CCCAGC 1 cut(s) 1113
BsgI GTGCAG 1 cut(s) 1454
Bsh1236I CGCG 1 cut(s) 1603
BshFI GGCC 2 cut(s) 631, 1554
BsiHKAI GWGCWC 1 cut(s) 1425
BsiHKCI CYCGRG 1 cut(s) 189
BsiSI CCGG 2 cut(s) 118, 495
BslFI GGGAC 2 cut(s) 466, 1964
BslI CCNNNNNNNGG 4 cut(s) 272, 638, 1005, 1417
BsmFI GGGAC 2 cut(s) 466, 1964
BsmI GAATGC 2 cut(s) 796, 1226
BsnI GGCC 2 cut(s) 631, 1554
BsoBI CYCGRG 1 cut(s) 189
Bsp119I TTCGAA 1 cut(s) 788
Bsp1286I GDGCHC 3 cut(s) 196, 1412, 1425
Bsp1407I TGTACA 2 cut(s) 512, 1089
Bsp1720I GCTNAGC 1 cut(s) 350
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 5 cut(s) 550, 1109, 1445, 1603, 1829
BspANI GGCC 2 cut(s) 631, 1554
BspCNI CTCAG 4 cut(s) 259, 728, 1600, 2008
BspFNI CGCG 1 cut(s) 1603
BspLI GGNNCC 2 cut(s) 74, 1880
BspMAI CTGCAG 2 cut(s) 648, 1385
BspPI GGATC 6 cut(s) 275, 757, 1151, 1873, 1886, 1912
BspT104I TTCGAA 1 cut(s) 788
BspTI CTTAAG 2 cut(s) 1018, 1883
BsrDI GCAATG 1 cut(s) 1103
BsrGI TGTACA 2 cut(s) 512, 1089
BsrI ACTGG 2 cut(s) 224, 261
BssECI CCNNGG 6 cut(s) 18, 484, 632, 839, 1563, 1983
BssT1I CCWWGG 3 cut(s) 18, 632, 1983
Bst2UI CCWGG 1 cut(s) 841
Bst4CI ACNGT 5 cut(s) 184, 512, 1089, 1237, 1564
Bst6I CTCTTC 1 cut(s) 969
BstAFI CTTAAG 2 cut(s) 1018, 1883
BstAUI TGTACA 2 cut(s) 512, 1089
BstBI TTCGAA 1 cut(s) 788
BstC8I GCNNGC 6 cut(s) 1111, 1226, 1445, 1552, 1556, 1605
BstDSI CCRYGG 2 cut(s) 18, 1563
BstFNI CGCG 1 cut(s) 1603
BstMWI GCNNNNNNNGC 2 cut(s) 1119, 1682
BstNI CCWGG 1 cut(s) 841
BstNSI RCATGY 3 cut(s) 1096, 1433, 2090
BstPAI GACNNNNGTC 1 cut(s) 1262
BstSCI CCNGG 1 cut(s) 839
BstSFI CTRYAG 3 cut(s) 459, 644, 1381
BstSLI GKGCMC 2 cut(s) 196, 1412
BstUI CGCG 1 cut(s) 1603
BstV1I GCAGC 6 cut(s) 242, 341, 1685, 1756, 1757, 1760
BstV2I GAAGAC 2 cut(s) 375, 396
BstX2I RGATCY 5 cut(s) 280, 593, 762, 1341, 1878
BstYI RGATCY 5 cut(s) 280, 593, 762, 1341, 1878
Bsu36I CCTNAGG 1 cut(s) 267
BsuI GTATCC 1 cut(s) 983
BsuRI GGCC 2 cut(s) 631, 1554
BtgI CCRYGG 2 cut(s) 18, 1563
BtsI GCAGTG 1 cut(s) 1314
BtsIMutI CAGTG 1 cut(s) 1314
Cac8I GCNNGC 6 cut(s) 1111, 1226, 1445, 1552, 1556, 1605
Cfr13I GGNCC 2 cut(s) 128, 1257
CseI GACGC 1 cut(s) 1619
Csp6I GTAC 6 cut(s) 173, 242, 513, 1090, 1386, 1836
CviQI GTAC 6 cut(s) 173, 242, 513, 1090, 1386, 1836
DraI TTTAAA 1 cut(s) 1188
DraIII CACNNNGTG 1 cut(s) 1564
EaeI YGGCCR 2 cut(s) 629, 1552
Eam1104I CTCTTC 1 cut(s) 969
EarI CTCTTC 1 cut(s) 969
Eco130I CCWWGG 3 cut(s) 18, 632, 1983
Eco32I GATATC 1 cut(s) 1914
Eco47I GGWCC 2 cut(s) 128, 1257
Eco57I CTGAAG 6 cut(s) 600, 720, 950, 1358, 1761, 2054
Eco81I CCTNAGG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 839
EcoRV GATATC 1 cut(s) 1914
EcoT14I CCWWGG 3 cut(s) 18, 632, 1983
EcoT22I ATGCAT 1 cut(s) 540
ErhI CCWWGG 3 cut(s) 18, 632, 1983
FalI AAGNNNNNCTT 2 cut(s) 408, 440
FaqI GGGAC 2 cut(s) 466, 1964
FauI CCCGC 4 cut(s) 543, 1102, 1452, 1596
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 6 cut(s) 231, 355, 1674, 1746, 1749, 1770
Fsp4HI GCNGC 6 cut(s) 231, 355, 1674, 1746, 1749, 1770
FspBI CTAG 3 cut(s) 147, 257, 1929
GluI GCNGC 6 cut(s) 231, 355, 1674, 1746, 1749, 1770
GsaI CCCAGC 1 cut(s) 1117
GsuI CTGGAG 1 cut(s) 707
HaeIII GGCC 2 cut(s) 631, 1554
HapII CCGG 2 cut(s) 118, 495
HgaI GACGC 1 cut(s) 1619
HincII GTYRAC 2 cut(s) 1537, 2061
HindII GTYRAC 2 cut(s) 1537, 2061
HindIII AAGCTT 3 cut(s) 870, 1199, 2112
HinfI GANTC 2 cut(s) 65, 940
HpaII CCGG 2 cut(s) 118, 495
HphI GGTGA 3 cut(s) 484, 825, 2120
Hpy166II GTNNAC 4 cut(s) 1312, 1537, 1567, 2061
Hpy8I GTNNAC 4 cut(s) 1312, 1537, 1567, 2061
Hpy99I CGWCG 1 cut(s) 240
HpyAV CCTTC 4 cut(s) 515, 946, 1894, 2000
HpyCH4III ACNGT 5 cut(s) 184, 512, 1089, 1237, 1564
HpyF10VI GCNNNNNNNGC 2 cut(s) 1119, 1682
Ksp22I TGATCA 1 cut(s) 474
LmnI GCTCC 1 cut(s) 1420
Lsp1109I GCAGC 6 cut(s) 242, 341, 1685, 1756, 1757, 1760
LweI GCATC 5 cut(s) 319, 525, 999, 1204, 1981
MaeI CTAG 3 cut(s) 147, 257, 1929
MaeIII GTNAC 7 cut(s) 732, 831, 972, 1028, 1073, 1644, 1732
MflI RGATCY 5 cut(s) 280, 593, 762, 1341, 1878
MhlI GDGCHC 3 cut(s) 196, 1412, 1425
MlsI TGGCCA 2 cut(s) 631, 1554
MluCI AATT 9 cut(s) 36, 466, 846, 900, 1102, 1125, 1487, 1543, 1623
MluNI TGGCCA 2 cut(s) 631, 1554
MmeI TCCRAC 1 cut(s) 1235
Mox20I TGGCCA 2 cut(s) 631, 1554
Mph1103I ATGCAT 1 cut(s) 540
MscI TGGCCA 2 cut(s) 631, 1554
MseI TTAA 7 cut(s) 813, 849, 899, 1019, 1187, 1845, 1884
MslI CAYNNNNRTG 2 cut(s) 1134, 1428
Msp20I TGGCCA 2 cut(s) 631, 1554
MspA1I CMGCKG 2 cut(s) 1479, 1550
MspCI CTTAAG 2 cut(s) 1018, 1883
MspI CCGG 2 cut(s) 118, 495
MspR9I CCNGG 1 cut(s) 841
Mva1269I GAATGC 2 cut(s) 796, 1226
MvaI CCWGG 1 cut(s) 841
MvnI CGCG 1 cut(s) 1603
MwoI GCNNNNNNNGC 2 cut(s) 1119, 1682
NcoI CCATGG 1 cut(s) 18
NlaIV GGNNCC 2 cut(s) 74, 1880
NmuCI GTSAC 1 cut(s) 831
NsiI ATGCAT 1 cut(s) 540
NspI RCATGY 3 cut(s) 1096, 1433, 2090
NspV TTCGAA 1 cut(s) 788
PctI GAATGC 2 cut(s) 796, 1226
PfeI GAWTC 2 cut(s) 65, 940
PflFI GACNNNGTC 1 cut(s) 1532
PflMI CCANNNNNTGG 1 cut(s) 638
PkrI GCNGC 6 cut(s) 232, 356, 1675, 1747, 1750, 1771
PshAI GACNNNNGTC 1 cut(s) 1262
PshBI ATTAAT 1 cut(s) 849
PsiI TTATAA 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 1113
PspGI CCWGG 1 cut(s) 839
PspN4I GGNNCC 2 cut(s) 74, 1880
PspPI GGNCC 2 cut(s) 128, 1257
PstI CTGCAG 2 cut(s) 648, 1385
PsuI RGATCY 5 cut(s) 280, 593, 762, 1341, 1878
PsyI GACNNNGTC 1 cut(s) 1532
PvuII CAGCTG 2 cut(s) 1479, 1550
RsaI GTAC 6 cut(s) 174, 243, 514, 1091, 1387, 1837
RsaNI GTAC 6 cut(s) 173, 242, 513, 1090, 1386, 1836
RseI CAYNNNNRTG 2 cut(s) 1134, 1428
SaqAI TTAA 7 cut(s) 813, 849, 899, 1019, 1187, 1845, 1884
SatI GCNGC 6 cut(s) 231, 355, 1674, 1746, 1749, 1770
Sau96I GGNCC 2 cut(s) 128, 1257
ScaI AGTACT 1 cut(s) 1387
ScrFI CCNGG 1 cut(s) 841
SduI GDGCHC 3 cut(s) 196, 1412, 1425
SfaNI GCATC 5 cut(s) 319, 525, 999, 1204, 1981
SfcI CTRYAG 3 cut(s) 459, 644, 1381
SfuI TTCGAA 1 cut(s) 788
SinI GGWCC 2 cut(s) 128, 1257
SmiMI CAYNNNNRTG 2 cut(s) 1134, 1428
SmlI CTYRAG 8 cut(s) 316, 398, 935, 1018, 1574, 1883, 1974, 2024
SmoI CTYRAG 8 cut(s) 316, 398, 935, 1018, 1574, 1883, 1974, 2024
Sse9I AATT 9 cut(s) 36, 466, 846, 900, 1102, 1125, 1487, 1543, 1623
SsiI CCGC 5 cut(s) 550, 1109, 1445, 1603, 1829
SspI AATATT 2 cut(s) 7, 826
SspMI CTAG 3 cut(s) 147, 257, 1929
StyD4I CCNGG 1 cut(s) 839
StyI CCWWGG 3 cut(s) 18, 632, 1983
TaaI ACNGT 5 cut(s) 184, 512, 1089, 1237, 1564
TaqI TCGA 6 cut(s) 45, 235, 387, 788, 1907, 2068
TasI AATT 9 cut(s) 36, 466, 846, 900, 1102, 1125, 1487, 1543, 1623
TatI WGTACW 4 cut(s) 172, 512, 1089, 1385
TfiI GAWTC 2 cut(s) 65, 940
Tru1I TTAA 7 cut(s) 813, 849, 899, 1019, 1187, 1845, 1884
Tru9I TTAA 7 cut(s) 813, 849, 899, 1019, 1187, 1845, 1884
TscAI CASTG 1 cut(s) 1314
TseFI GTSAC 1 cut(s) 831
TseI GCWGC 6 cut(s) 230, 354, 1673, 1745, 1748, 1769
Tsp45I GTSAC 1 cut(s) 831
TspDTI ATGAA 9 cut(s) 17, 29, 95, 692, 965, 1118, 2019, 2085, 2092
TspRI CASTG 1 cut(s) 1314
Tth111I GACNNNGTC 1 cut(s) 1532
Van91I CCANNNNNTGG 1 cut(s) 638
Vha464I CTTAAG 2 cut(s) 1018, 1883
VpaK11BI GGWCC 2 cut(s) 128, 1257
VspI ATTAAT 1 cut(s) 849
XapI RAATTY 4 cut(s) 36, 466, 1125, 1623
XceI RCATGY 3 cut(s) 1096, 1433, 2090
XspI CTAG 3 cut(s) 147, 257, 1929
ZrmI AGTACT 1 cut(s) 1387
Zsp2I ATGCAT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.