RchiOBHm_Chr3g0473441

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19298149 .. 19301261
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43912

Sequence Viewer

Length: 3009 bp
ATGGAAGAAGACAACAATAGAATTCTGCCACTTTTTTATTATGTGGATCCTACTGATGTACGATATCAGAAGAGGAGTTTCGGAGATGCTTTTACTGAGCATGAAAAATCAGAGCGACACAGATCAGAGAAGGTGCAGCAGTGGAGAGATGCTTTAAAAAAAGTGGCAAATTTCTCTGGATGGCATACACTGAATTATAAAACTGAAAGAGAACTTTTGGATGCCATTGAGGAATCTGTGTGCAATAAATTACGACCTACTGAACTTCAGTTTAAATTGTCCATCGGAGGTTTTGAAGCATTTAAAGCAACAAAACAAGCCATTGATGAGGTCATGAATGTGCTAAAAGATGATAAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTCGGAAAAACGACAATGGTGAAATATGTTGGAGCACTAGCCCAAGAAAGTGGGCTTTTTAATCAAGTGATTATGGCTGTCGTATCCCAAAACCCCGACTTGATAAAAATTCAAGAGACATTTGCAGAACTGCTGGGCTTTAAATTGGAGGAGAAGACAGAAATTGGAAGAGCCATTAAATTGAAGGAGAAGATAATGAGAGGAACCGGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGACTTTTCAAGCATAGGAATTCCAAGCCACAACGAACTTCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAGATTGACTGTTTGTCAGATCATGGAGAGCCATGCAAAAATTCATCTCAATATCTTATCAAAAGAAGATTCTTGGAGCTTATTTGTGAAGGAAGCAAGGAAATCTTTTGACAAATCTTCCAATTTCTATGTTGTAGCGAGTGAGGTGGCAAGAGAATGCGCCGGTCTACCAGTTACCTTGATAGCAGTTGCGAGGGCCCTTCGAGATGAAGGTTTGGACGGATGGAAAGAAGCTGCTCGACGACTGAAAGCATCTCAACCTCCCATCCCTGAAGATGAGGGAGATGTTTTCAAATGCATAAAGTTGAGCTATGATTACTTGAAATATGATGATTCCAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCAGAAGATTATGATATCCCAATTGACTACTTGTTCAAGTATGGAATCGGGAAAGGAATGTTTCAAGATTCAAACTTGCTAGAAGCCCGAGCCACAACATATTTAGTGGTGAAGGCCCTTACAGATTCTAGCTTGCTTTTGGACGCTGGAGATGACCGATATGTAAGGATGCATGATGTCATTCGGGATATGGCAATCTTAATTTCGTTATCAGAAGGCCTGCGGTTCTTGGTGAAAGCTGGCTGTGAATTGAAGAATTGGCCACAGATTGATGCACACAAAGGCTACTCTGCAATCTCACTAATGGAGAACGAAATTTGCAAGCTACCCGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTGCAATACAATGCTTCATTAAATGATATCCCAAACTCTTTCTTCCAATGTCAGAAAGAATTAAGAGTCTTAGATCTTGGCGGCACTGGTATTTCATTACTACCCCATTCAATCAGTTTCCTAACGAACCTTCAAGCTTTGTATTTAGATTATTGCCGCAACATAATTGACACTTCTGTAATCGGAAAACTTCACAAGCTTGAGATTCTTAGTATGAGAGGAACTGATCTGGAAGAATTGTCAAGAGAAATAGGACAATTGACCAATCTAAGGATGCTTGATGTCAGTGGTGCTGGATTTATTTGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAGCTGTACATGCAATGTGCATTTTGGGACTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGCTGGTTTATCATATTTACGCAATTTGGAAGTTTGCATATCAGATGAAAATTGCATCCCTAGAAGTGTTGAGGTCGAACCGAATTGGGATTACTTCGATATTCGCATTTGCAGCGACGGTGATGGAAAGATTGAAATTATGAGCTATCTATCTGGTGATTCTGGTCGTAAGTGTAGATCCTTGGGTCTTCATAGTACTGACACAACCATAGGAACCTTTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAAAACTGAGAGGCTGAGTTATGAAGAATGCACAGGGTTGAGTAACATTCTTGTGGAATATGACCGTGGGAGGTTACATGGACTGAAAGTTCTCTCTGTAAGATATTGCTGTGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCCAGATATGCCTGTGTTTGAGAACTTGGAAGAGTTGTATCTGAAAAATCTGGATGACCTGAAGCAGTTATGTGTTGGTGAGTTACCACCTGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCGAACGGTGTCGTCACTTGGGGAATGTGCTGTTGCCATCAAAATTGTTGCAGAAACTACCAAATCTGAAAAAACTAATATGTGGGGTAAGTAGAGTGGAATATGTGTTTGGATGTGAAGGATTTGAGCCGCCGGATCAAACAAATCTAAGAGAGATGAAGTTGTTCGATCTAGACGCAGTGAGAAGCATATGTAATGGTCTTGCTCCACGTGGAATGTTCCAGGCTCTAAAGGAATTGGCCATTGAGAGGTGCAACATCCAGGGAAGTCTCTTCACAATTGATGAAGCTCAGTGTCTTTTTCAATTGGAAAGCCTTGTTGTATCCAGTTGCCCTGTCTTGGAAAGAGTAATCGAAGCAAGGAGGGAAACATTGAACAGCAAGAAGACCGTTCTTCCAAAAATGAAGAACTTATGTTTGCGATATCTTCCAATGTTGTACGAGGGAAGTGCTACTGTTGATTTTGAGTGTCCTTCATTGGAAAACTTGCTTCTGAGGGACTGCCCCCACTTGTCATTTCAATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCCAGTCAATGAAGATTGGAGTCTGTTTCTCCGATTTAAGTATGCCGCTGGGTCATAA

Protein Analysis

1002

Amino Acids

113.58

Weight (kDa)

5.49

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 88 1.8e-14 TIR domain
NB-ARC PF00931 105 - 270 1.5e-28 NB-ARC domain
LRR_8 PF13855 475 - 534 7e-08 Leucine rich repeat
LRR_14 PF23598 500 - 598 8.1e-11 Leucine-rich repeat region
LRR_14 PF23598 542 - 610 7.7e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 707 - 825 4.6e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 768 - 910 2.8e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 850 - 970 3.1e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 198
AasI GACNNNNNNGTC 1 cut(s) 2108
AccI GTMKAC 2 cut(s) 375, 877
AccIII TCCGGA 1 cut(s) 2098
AciI CCGC 5 cut(s) 1300, 1524, 1600, 2528, 2997
AclWI GGATC 7 cut(s) 41, 54, 595, 608, 2049, 2377, 2541
AcoI YGGCCR 2 cut(s) 1337, 2637
AcsI RAATTY 6 cut(s) 21, 169, 498, 654, 750, 1392
AcuI CTGAAG 3 cut(s) 251, 1002, 2357
AcvI CACGTG 1 cut(s) 2609
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 4 cut(s) 60, 1799, 2074, 2837
AfiI CCNNNNNNNGG 3 cut(s) 1713, 2646, 2737
AflIII ACRYGT 1 cut(s) 2270
AhdI GACNNNNNGTC 1 cut(s) 723
AjnI CCWGG 3 cut(s) 2365, 2619, 2658
AjuI GAANNNNNNNTTGG 6 cut(s) 1040, 1072, 1845, 1877, 2490, 2522
AloI GAACNNNNNNTCC 2 cut(s) 2199, 2231
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 2 cut(s) 500, 2672
AlwI GGATC 7 cut(s) 41, 54, 595, 608, 2049, 2377, 2541
Ama87I CYCGRG 1 cut(s) 1164
Aor13HI TCCGGA 1 cut(s) 2098
AoxI GGCC 6 cut(s) 357, 906, 1191, 1294, 1337, 2637
ApaI GGGCCC 1 cut(s) 910
ApeKI GCWGC 4 cut(s) 136, 944, 1068, 1989
ApoI RAATTY 6 cut(s) 21, 169, 498, 654, 750, 1392
Asp700I GAANNNNTTC 1 cut(s) 2561
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 3 cut(s) 906, 907, 1192
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 6 cut(s) 421, 1198, 1321, 2009, 2045, 2366
AvaI CYCGRG 1 cut(s) 1164
BaeGI GKGCMC 1 cut(s) 910
BalI TGGCCA 2 cut(s) 1339, 2639
BamHI GGATCC 2 cut(s) 46, 600
BanII GRGCYC 1 cut(s) 910
BbrPI CACGTG 1 cut(s) 2609
BbsI GAAGAC 4 cut(s) 15, 551, 2057, 2789
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 4 cut(s) 148, 931, 1055, 2001
BccI CCATC 8 cut(s) 174, 290, 927, 983, 1765, 1832, 1994, 2443
BcgI CGANNNNNNTGC 2 cut(s) 2828, 2862
BciT130I CCWGG 3 cut(s) 2367, 2621, 2660
BciVI GTATCC 2 cut(s) 484, 2731
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 2 cut(s) 500, 2672
BfaI CTAG 5 cut(s) 428, 1157, 1206, 1938, 2570
BfmI CTRYAG 1 cut(s) 2377
BfuI GTATCC 2 cut(s) 484, 2731
BglII AGATCT 1 cut(s) 1516
BisI GCNGC 8 cut(s) 137, 945, 1069, 1525, 1600, 1990, 2528, 2997
BlsI GCNGC 8 cut(s) 138, 946, 1070, 1526, 1601, 1991, 2529, 2998
BmcAI AGTACT 1 cut(s) 2074
Bme1390I CCNGG 4 cut(s) 598, 2367, 2621, 2660
BmeRI GACNNNNNGTC 1 cut(s) 723
BmeT110I CYCGRG 1 cut(s) 1164
BmgT120I GGNCC 3 cut(s) 906, 907, 1192
BmiI GGNNCC 6 cut(s) 48, 595, 602, 908, 2092, 2951
BmrFI CCNGG 4 cut(s) 598, 2367, 2621, 2660
BmrI ACTGGG 2 cut(s) 1831, 2948
BmsI GCATC 9 cut(s) 76, 139, 211, 671, 971, 1236, 1339, 1707, 1941
BmuI ACTGGG 2 cut(s) 1831, 2948
BpiI GAAGAC 4 cut(s) 15, 551, 2057, 2789
BpmI CTGGAG 2 cut(s) 1245, 1415
BpuEI CTTGAG 1 cut(s) 1664
BpuMI CCSGG 1 cut(s) 598
BsaAI YACGTR 1 cut(s) 2609
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 2058, 2191, 2659
BsaWI WCCGGW 1 cut(s) 2098
Bsc4I CCNNNNNNNGG 3 cut(s) 1713, 2646, 2737
Bse118I RCCGGY 1 cut(s) 872
Bse1I ACTGG 6 cut(s) 881, 1534, 1826, 2108, 2724, 2954
Bse3DI GCAATG 1 cut(s) 1811
BseAI TCCGGA 1 cut(s) 2098
BseBI CCWGG 3 cut(s) 2367, 2621, 2660
BseDI CCNNGG 3 cut(s) 2058, 2191, 2659
BseLI CCNNNNNNNGG 3 cut(s) 1713, 2646, 2737
BseMI GCAATG 1 cut(s) 1811
BseMII CTCAG 5 cut(s) 87, 2124, 2132, 2702, 2882
BseNI ACTGG 6 cut(s) 881, 1534, 1826, 2108, 2724, 2954
BseRI GAGGAG 2 cut(s) 88, 554
BseSI GKGCMC 1 cut(s) 910
BseXI GCAGC 4 cut(s) 148, 931, 1055, 2001
BseYI CCCAGC 2 cut(s) 523, 2999
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 6 cut(s) 359, 908, 1193, 1296, 1339, 2639
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 1164
BsiSI CCGG 4 cut(s) 597, 873, 2099, 2531
BslFI GGGAC 2 cut(s) 1832, 2909
BslI CCNNNNNNNGG 3 cut(s) 1713, 2646, 2737
BsmAI GTCTC 2 cut(s) 500, 2672
BsmFI GGGAC 2 cut(s) 1832, 2909
BsmI GAATGC 3 cut(s) 872, 2159, 2266
BsnI GGCC 6 cut(s) 359, 908, 1193, 1296, 1339, 2639
BsoBI CYCGRG 1 cut(s) 1164
Bsp120I GGGCCC 1 cut(s) 906
Bsp1286I GDGCHC 2 cut(s) 427, 910
Bsp13I TCCGGA 1 cut(s) 2098
Bsp1407I TGTACA 1 cut(s) 1797
BspACI CCGC 5 cut(s) 1300, 1524, 1600, 2528, 2997
BspANI GGCC 6 cut(s) 359, 908, 1193, 1296, 1339, 2639
BspCNI CTCAG 5 cut(s) 88, 2125, 2133, 2701, 2883
BspEI TCCGGA 1 cut(s) 2098
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 6 cut(s) 48, 595, 602, 908, 2092, 2951
BspMAI CTGCAG 1 cut(s) 2381
BspPI GGATC 7 cut(s) 41, 54, 595, 608, 2049, 2377, 2541
BspQI GCTCTTC 2 cut(s) 553, 1785
BsrDI GCAATG 1 cut(s) 1811
BsrFI RCCGGY 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 1797
BsrI ACTGG 6 cut(s) 881, 1534, 1826, 2108, 2724, 2954
BssAI RCCGGY 1 cut(s) 872
BssECI CCNNGG 3 cut(s) 2058, 2191, 2659
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 2058
Bst2UI CCWGG 3 cut(s) 2367, 2621, 2660
Bst4CI ACNGT 6 cut(s) 721, 1997, 2192, 2406, 2788, 2854
Bst6I CTCTTC 7 cut(s) 65, 553, 623, 1404, 1785, 2301, 2675
BstACI GRCGYC 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 1797
BstBAI YACGTR 1 cut(s) 2609
BstC8I GCNNGC 4 cut(s) 1211, 1298, 1318, 1400
BstDEI CTNAG 9 cut(s) 96, 1513, 1652, 1712, 2133, 2141, 2546, 2688, 2891
BstDSI CCRYGG 1 cut(s) 2191
BstHHI GCGC 1 cut(s) 872
BstMAI GTCTC 2 cut(s) 500, 2672
BstMWI GCNNNNNNNGC 3 cut(s) 305, 1801, 1989
BstNI CCWGG 3 cut(s) 2367, 2621, 2660
BstNSI RCATGY 2 cut(s) 1804, 2274
BstSCI CCNGG 4 cut(s) 596, 2365, 2619, 2658
BstSFI CTRYAG 1 cut(s) 2377
BstSLI GKGCMC 1 cut(s) 910
BstV1I GCAGC 4 cut(s) 148, 931, 1055, 2001
BstV2I GAAGAC 4 cut(s) 15, 551, 2057, 2789
BstX2I RGATCY 5 cut(s) 46, 600, 1516, 2054, 2369
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 5 cut(s) 46, 600, 1516, 2054, 2369
BsuI GTATCC 2 cut(s) 484, 2731
BsuRI GGCC 6 cut(s) 359, 908, 1193, 1296, 1339, 2639
BtgI CCRYGG 1 cut(s) 2191
BtsI GCAGTG 2 cut(s) 146, 2583
BtsIMutI CAGTG 6 cut(s) 146, 188, 1527, 1735, 2583, 2696
Cac8I GCNNGC 4 cut(s) 1211, 1298, 1318, 1400
CciI TCATGA 1 cut(s) 333
CfoI GCGC 1 cut(s) 872
Cfr10I RCCGGY 1 cut(s) 872
Cfr13I GGNCC 3 cut(s) 906, 907, 1192
CseI GACGC 3 cut(s) 379, 1229, 2582
Csp6I GTAC 4 cut(s) 59, 1798, 2073, 2836
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 9 cut(s) 101, 334, 733, 743, 1053, 1250, 1801, 2204, 2271
CviQI GTAC 4 cut(s) 59, 1798, 2073, 2836
DdeI CTNAG 9 cut(s) 96, 1513, 1652, 1712, 2133, 2141, 2546, 2688, 2891
DraI TTTAAA 4 cut(s) 156, 274, 304, 532
DrdI GACNNNNNNGTC 1 cut(s) 2108
DriI GACNNNNNGTC 1 cut(s) 723
DseDI GACNNNNNNGTC 1 cut(s) 2108
EaeI YGGCCR 2 cut(s) 1337, 2637
Eam1104I CTCTTC 7 cut(s) 65, 553, 623, 1404, 1785, 2301, 2675
Eam1105I GACNNNNNGTC 1 cut(s) 723
EarI CTCTTC 7 cut(s) 65, 553, 623, 1404, 1785, 2301, 2675
Eco130I CCWWGG 1 cut(s) 2058
Eco147I AGGCCT 1 cut(s) 1296
Eco24I GRGCYC 1 cut(s) 910
Eco32I GATATC 5 cut(s) 65, 1093, 1471, 1770, 2822
Eco57I CTGAAG 3 cut(s) 251, 1002, 2357
Eco72I CACGTG 1 cut(s) 2609
Eco88I CYCGRG 1 cut(s) 1164
EcoO109I RGGNCCY 3 cut(s) 906, 907, 1192
EcoRI GAATTC 2 cut(s) 21, 654
EcoRII CCWGG 3 cut(s) 2365, 2619, 2658
EcoRV GATATC 5 cut(s) 65, 1093, 1471, 1770, 2822
EcoT14I CCWWGG 1 cut(s) 2058
EcoT22I ATGCAT 2 cut(s) 1010, 1251
EcoT38I GRGCYC 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 2058
FaeI CATG 9 cut(s) 104, 337, 736, 746, 1056, 1253, 1804, 2207, 2274
FalI AAGNNNNNCTT 2 cut(s) 799, 831
FaqI GGGAC 2 cut(s) 1832, 2909
FatI CATG 9 cut(s) 100, 333, 732, 742, 1052, 1249, 1800, 2203, 2270
FauNDI CATATG 1 cut(s) 2588
FblI GTMKAC 2 cut(s) 375, 877
Fnu4HI GCNGC 8 cut(s) 137, 945, 1069, 1525, 1600, 1990, 2528, 2997
FriOI GRGCYC 1 cut(s) 910
Fsp4HI GCNGC 8 cut(s) 137, 945, 1069, 1525, 1600, 1990, 2528, 2997
FspBI CTAG 5 cut(s) 428, 1157, 1206, 1938, 2570
GlaI GCGC 1 cut(s) 871
GluI GCNGC 8 cut(s) 137, 945, 1069, 1525, 1600, 1990, 2528, 2997
GsaI CCCAGC 2 cut(s) 527, 3003
GsuI CTGGAG 2 cut(s) 1245, 1415
HaeIII GGCC 6 cut(s) 359, 908, 1193, 1296, 1339, 2639
HapII CCGG 4 cut(s) 597, 873, 2099, 2531
HgaI GACGC 3 cut(s) 379, 1229, 2582
HhaI GCGC 1 cut(s) 872
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 9 cut(s) 104, 337, 736, 746, 1056, 1253, 1804, 2207, 2274
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HincII GTYRAC 1 cut(s) 2113
HindII GTYRAC 1 cut(s) 2113
HindIII AAGCTT 2 cut(s) 1578, 1640
HpaII CCGG 4 cut(s) 597, 873, 2099, 2531
HphI GGTGA 6 cut(s) 421, 1198, 1321, 2009, 2045, 2366
Hpy166II GTNNAC 3 cut(s) 376, 878, 2113
Hpy8I GTNNAC 3 cut(s) 376, 878, 2113
Hpy99I CGWCG 3 cut(s) 395, 954, 1997
HpyCH4III ACNGT 6 cut(s) 721, 1997, 2192, 2406, 2788, 2854
HpyCH4IV ACGT 1 cut(s) 2608
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 1801, 1989
HpyF3I CTNAG 9 cut(s) 96, 1513, 1652, 1712, 2133, 2141, 2546, 2688, 2891
HpySE526I ACGT 1 cut(s) 2608
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 9 cut(s) 104, 337, 736, 746, 1056, 1253, 1804, 2207, 2274
HspAI GCGC 1 cut(s) 870
Kpn2I TCCGGA 1 cut(s) 2098
LguI GCTCTTC 2 cut(s) 553, 1785
LmnI GCTCC 3 cut(s) 422, 786, 2608
Lsp1109I GCAGC 4 cut(s) 148, 931, 1055, 2001
LweI GCATC 9 cut(s) 76, 139, 211, 671, 971, 1236, 1339, 1707, 1941
MaeI CTAG 5 cut(s) 428, 1157, 1206, 1938, 2570
MaeII ACGT 1 cut(s) 2608
MaeIII GTNAC 5 cut(s) 883, 2168, 2199, 2358, 2411
MfeI CAATTG 4 cut(s) 1098, 1700, 2676, 2702
MflI RGATCY 5 cut(s) 46, 600, 1516, 2054, 2369
MhlI GDGCHC 2 cut(s) 427, 910
MlsI TGGCCA 2 cut(s) 1339, 2639
MluNI TGGCCA 2 cut(s) 1339, 2639
MlyI GAGTC 3 cut(s) 381, 1518, 2980
MmeI TCCRAC 2 cut(s) 373, 400
Mox20I TGGCCA 2 cut(s) 1339, 2639
Mph1103I ATGCAT 2 cut(s) 1010, 1251
MroI TCCGGA 1 cut(s) 2098
MroXI GAANNNNTTC 1 cut(s) 2561
MscI TGGCCA 2 cut(s) 1339, 2639
MslI CAYNNNNRTG 2 cut(s) 338, 2177
Msp20I TGGCCA 2 cut(s) 1339, 2639
MspA1I CMGCKG 1 cut(s) 2999
MspI CCGG 4 cut(s) 597, 873, 2099, 2531
MspR9I CCNGG 4 cut(s) 598, 2367, 2621, 2660
MunI CAATTG 4 cut(s) 1098, 1700, 2676, 2702
Mva1269I GAATGC 3 cut(s) 872, 2159, 2266
MvaI CCWGG 3 cut(s) 2367, 2621, 2660
MwoI GCNNNNNNNGC 3 cut(s) 305, 1801, 1989
NciI CCSGG 1 cut(s) 598
NdeI CATATG 1 cut(s) 2588
NlaIII CATG 9 cut(s) 104, 337, 736, 746, 1056, 1253, 1804, 2207, 2274
NlaIV GGNNCC 6 cut(s) 48, 595, 602, 908, 2092, 2951
NmuCI GTSAC 1 cut(s) 2411
NsiI ATGCAT 2 cut(s) 1010, 1251
NspI RCATGY 2 cut(s) 1804, 2274
PagI TCATGA 1 cut(s) 333
PceI AGGCCT 1 cut(s) 1296
PciI ACATGT 1 cut(s) 2270
PciSI GCTCTTC 2 cut(s) 553, 1785
PctI GAATGC 3 cut(s) 872, 2159, 2266
PdmI GAANNNNTTC 1 cut(s) 2561
PfeI GAWTC 8 cut(s) 233, 779, 1043, 1122, 1145, 1202, 1648, 2036
PkrI GCNGC 8 cut(s) 138, 946, 1070, 1526, 1601, 1991, 2529, 2998
PleI GAGTC 3 cut(s) 380, 1517, 2979
PmaCI CACGTG 1 cut(s) 2609
PmlI CACGTG 1 cut(s) 2609
PpsI GAGTC 3 cut(s) 380, 1517, 2979
Ppu21I YACGTR 1 cut(s) 2609
PscI ACATGT 1 cut(s) 2270
PsiI TTATAA 1 cut(s) 198
Psp6I CCWGG 3 cut(s) 2365, 2619, 2658
PspCI CACGTG 1 cut(s) 2609
PspFI CCCAGC 2 cut(s) 523, 2999
PspGI CCWGG 3 cut(s) 2365, 2619, 2658
PspN4I GGNNCC 6 cut(s) 48, 595, 602, 908, 2092, 2951
PspOMI GGGCCC 1 cut(s) 906
PspPI GGNCC 3 cut(s) 906, 907, 1192
PstI CTGCAG 1 cut(s) 2381
PsuI RGATCY 5 cut(s) 46, 600, 1516, 2054, 2369
RsaI GTAC 4 cut(s) 60, 1799, 2074, 2837
RsaNI GTAC 4 cut(s) 59, 1798, 2073, 2836
RseI CAYNNNNRTG 2 cut(s) 338, 2177
SapI GCTCTTC 2 cut(s) 553, 1785
SatI GCNGC 8 cut(s) 137, 945, 1069, 1525, 1600, 1990, 2528, 2997
Sau96I GGNCC 3 cut(s) 906, 907, 1192
ScaI AGTACT 1 cut(s) 2074
SchI GAGTC 3 cut(s) 381, 1518, 2980
ScrFI CCNGG 4 cut(s) 598, 2367, 2621, 2660
SduI GDGCHC 2 cut(s) 427, 910
SfaNI GCATC 9 cut(s) 76, 139, 211, 671, 971, 1236, 1339, 1707, 1941
SfcI CTRYAG 1 cut(s) 2377
SmiMI CAYNNNNRTG 2 cut(s) 338, 2177
SmlI CTYRAG 1 cut(s) 1643
SmoI CTYRAG 1 cut(s) 1643
SseBI AGGCCT 1 cut(s) 1296
SsiI CCGC 5 cut(s) 1300, 1524, 1600, 2528, 2997
SspI AATATT 1 cut(s) 1861
SspMI CTAG 5 cut(s) 428, 1157, 1206, 1938, 2570
StuI AGGCCT 1 cut(s) 1296
StyD4I CCNGG 4 cut(s) 596, 2365, 2619, 2658
StyI CCWWGG 1 cut(s) 2058
TaaI ACNGT 6 cut(s) 721, 1997, 2192, 2406, 2788, 2854
TaiI ACGT 1 cut(s) 2611
TaqI TCGA 7 cut(s) 913, 949, 1953, 1974, 2400, 2565, 2751
TaqII GACCGA 1 cut(s) 1248
TatI WGTACW 2 cut(s) 1797, 2072
TauI GCSGC 4 cut(s) 1527, 1602, 2530, 2999
TfiI GAWTC 8 cut(s) 233, 779, 1043, 1122, 1145, 1202, 1648, 2036
TscAI CASTG 6 cut(s) 146, 195, 1534, 1735, 2583, 2696
TseFI GTSAC 1 cut(s) 2411
TseI GCWGC 4 cut(s) 136, 944, 1068, 1989
Tsp45I GTSAC 1 cut(s) 2411
TspGWI ACGGA 2 cut(s) 393, 945
TspRI CASTG 6 cut(s) 146, 195, 1534, 1735, 2583, 2696
XapI RAATTY 6 cut(s) 21, 169, 498, 654, 750, 1392
XbaI TCTAGA 1 cut(s) 2569
XceI RCATGY 2 cut(s) 1804, 2274
XmiI GTMKAC 2 cut(s) 375, 877
XmnI GAANNNNTTC 1 cut(s) 2561
XspI CTAG 5 cut(s) 428, 1157, 1206, 1938, 2570
ZrmI AGTACT 1 cut(s) 2074
Zsp2I ATGCAT 2 cut(s) 1010, 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.