RchiOBHm_Chr3g0473561

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000085
Physical Location & Seq
Forward (+)
19368356 .. 19379287
10932 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43922

Sequence Viewer

Length: 3573 bp
ATGGAGTTCTACCAGTACGTCTTTGAGGCGATGAGGATGATTATTGTGATTAGTGCGGTAACGGTGCAATGGTTCGGTGTCATAGCCAGTCTTAAGTGGATATGGAAGAATTGGTTCATTATTGTGGTGATTGTGACATGGAACGTAATAGCCACTCTTGGGTGGATATGGAAGCATAGGTTCAGTTACATGGTGGAAACCCCAGAGCCAATAGCAGAGGGTAATCAAGAACCGGTTGACGTCGATGCCTCTACATCATCAGCTCCTAGGTGGAAGCACGATGTGTTTTTGAGTTTCAGGGGTGTAGACGCTCGCAAAGGTATTGCATCCGAAATATACTATCGACTGCAAACCAGGAAAGGAATTAAAATTTTCATGGATGACCCGGACCTTCAAGTAGGGAATGTTATTTCTCCCACTCTCCTAACAGCAATTAAAGAATCAAGGTTTGCAATTATTGTTCTCTCTCCAAACTATGCCTCTTCCACTTGGTGTTTGGAGGAACTTAGATACATTTGTGAATGCATGAAAGAAGACGAGGACAGAATTCTGCCACTTTTTTATTATGTGAATCCTACTGATGTACGATATCAGAAGAGCAGTTTCGGTGATGCTTTCACTAAGCATGAAAATTCTGGGCGATACGGATTAGAGGAGATGCGGCAGTGGAGAGCTGCTTTAAACAAAGTGGCAAATTTCTCTGGATGGGACACAAAGAATTATAAAACTGAAAGAGCACTTTTGGATGCCGTTGAGGAAACTGTGTGTGATAAATTACGAGCTACTGAACTTGAGCTTAAAATGTCCATTGGAGGTTTTGAAGTATTTGAAGCAACAAAACAAGCCATTGATCAGGTCATGAATGCGCTGAAAGATAATGAGGCCACTACCATTGGAGTCTACGGAATGGGGGGCGTCGGGAAGACGACAATGGTGAATTATGTCGGTGTACAAGCCCAAGAAAATAGGCTTTTTGATAAAGTGATTTTGGCTGTCGTATCCCAAAACCCCGACTTGATGAAAATTCAACAGACATTTGCAGAAATGCTGGGCTTTGAACTCAAGGAGAAGACAGAAATTGTAAGAGCCCGTGAATTGAAGAAGAAAATAATGAGAGGAACAAGGATCCTCATAATCTTGGATGACATTTGGAAGAGAATAGAGTTTTCAGACATTGGAATTCCGAGCCACAACGATCTTCAAAGATGCAATTCCAAAGTTCTACTGACCACCAGAAAATTGGATGTTTGCCGTTCCATGGATTGCCATGCAAACATACGTCTCAATATCTTATCAGAAAAAGATTCTCGGAGCTTATTTGTGAAGGAAGCAAGGAAGTACCCTCTTGACAAATCATCCAATTTCTATGATGTAGCGAGTGAGGTGGCTAGAGAATGCGCTGGTCTTCCTATTGCTTTGATAGCAGTTGCGAGGGCCCTTCGAGGTGAAGGTTTGGACAGATGGAAAGAAGCTGCTCGACGGCTGAAAGCGTCTCAACCCCCCGTCCTTGAAGCTGAGGAAGATGTGTTCAAATGCATAAAGTTAAGCTATGATTACTTGAAATCTGATGTTTCGAAATCATGCTTCTTGCTTTGCTGCCTGTTCCCTGAAGATTATGATATCCCAATTGAATACTTGCTCAATTATGGAATTGGGAAAGGAATGTTTCAAGATTCTGACATGCTAGACGCCCGCGCCACAACAAATTCAGCGGTGAAGGCCCTTAAAGACTCTAGCTTGCTTTTGGACTCTAGACATGACGTATGTGTAAGGATGCATGATGTCATTCGGGATATGGCAATATTGATATCATTATCTGAAGACGGCCCGCGGTTCTTGGTGAAAGTTGGCTGTGAATTGAAGAATTGGCCGAGGATTGATGCACGTAAGGGCTACTCTGCAATCTCACTAATGAAGAACAAAATTTGCAAGCTACCCGAAGAGTTGGTATGTTCAAGTCTCCAGATTTTATTACTGCAAGACAATGCTTCATTAAATGATATCCCTAACTCTTTCTTCCAAAATCAGAAAGAATTAAGAGTCTTGGATCTTAGCCGCACTGGTATTTCATTACTACCCCAATCAATCAGTTTCCTAACCAACCTTCAAGCTTTGTATTTAGATTACTGCAAGAACATAATTGACATTTCTGTAATCGGAAAACTTAACAAGCTTGAGATTCTTAGTATGAGAGGAAATGCTCTGAAAGAATTGCCAAGAGAAATAGGACAGTTGACCAGTCTAAGGATGCTAGATATCACTAGAGCTGATAGATTCCTTTTCACATCCACGAATGGATGTATTGGCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTACATGCAATGTGGATTTTGGGAGTGGGGGAGTAAAATTGATGGAGAAGGAGAAGAAACTAATATTGGCTTTGATGAATTAGTTGGTTTATCATATTTAAGCATTTTGAAAGTTTGGATGTCAGATGCAAATTGCATCCCTATAAGTGTTGAGGTCGAACCGAATTGGGTTTACTTTGATATTACTATTTGCAGCAACAGTTATTTCACTTCGAACAGTCAATCTGGTCATAATTGTAGATCGTTGAGTTTTAATGGAACTGACACTACCATAGGAATCTGTCCGGACTGGTTTGTCAACGCTGTGGTAAAGAATACTGAGAGGCTACTTTATAGTTACTGCAAAGGGTTAAGTAACATTCTTGTGGAATATGACCGTGGAAGGTTACATGGATTGAAAGTTCTCTCTGTAATTGGTTGCCATGAGAACTTGAAAGAATTGATGAATGCAATAACATGTGTTCTAGATATGCCTGTGTTTGAGAATTTGGAAGAGTTGTATCTGGAATACCTGGATTACTTGAAGCAGTTATGTGTTGGTGAGTTACCACCAGGATCTCTCTGCAGTCTGAAATTATTGAAGGTCCTTAAGTGTCCTATCTTGGGGAATGTACTGTTGCCATCTAAATTGTTGCAGCAACTACCAAATCTGGAAAAACTAATCTGTGAGTCAAGTGAAGTGGAACATGTGTTTGGATGTGAAGGTTTTGAGCCAGATCAAACAAATCTGAGAGAGATGGATTTACGGTATCTAGATGTCGTAACAAGCATATGTAATGGTCCTGCTCCACATGGAATGTTCCAGGCTCTAAAGAAATTGGTCATTCGTAATTGCAACTTCCAGGGAAGCCTCTTCACATTTGATGTAGCTCAGTGTCTTTTTCAATTGGAAGACCTTATTGTATCCGAATGCCCTGTCTTGGAAAGAGTAATTGAAGAAAGTAGGGAAACATTGAACAACAAGAAGACCATTCTTCCAAAATTGAAGAACCTAGCTTTGGAAGATCTTCCAATGTTGTACAAGGGAAGTGCTACTATTGATTTTGAGTGTCCTTCATTGGAAAAACTGAGTCTGTTTGGCTGCCCCCACTTGTCATTTCCATCCTCTGCTTCTGACTACTTCCACAGCAGGAACCAAGTCGTTTTCAATGATGCTCCTCGTCATTCTCTGCTACTCAGCAAACTAGGGTATAAGGGATTCAAAATATCAAGTTGGTAG

Protein Analysis

1190

Amino Acids

135.24

Weight (kDa)

5.82

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 92 - 263 6.1e-46 TIR domain
TIR_2 PF13676 95 - 189 1.8e-15 TIR domain
NB-ARC PF00931 280 - 445 3e-30 NB-ARC domain
LRR_14 PF23598 644 - 791 2.1e-12 Leucine-rich repeat region
LRR_8 PF13855 653 - 710 2.2e-08 Leucine rich repeat
LRR_RPS2 PF23247 948 - 1089 1.7e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1030 - 1152 1e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AasI GACNNNNNNGTC 1 cut(s) 2648
AatII GACGTC 1 cut(s) 243
AccI GTMKAC 2 cut(s) 306, 900
AccII CGCG 2 cut(s) 1695, 1831
AccIII TCCGGA 1 cut(s) 2638
AciI CCGC 7 cut(s) 56, 661, 1693, 1712, 1829, 1831, 2056
AclWI GGATC 4 cut(s) 1120, 1133, 2055, 2917
AcoI YGGCCR 1 cut(s) 1868
AcsI RAATTY 9 cut(s) 369, 546, 631, 694, 1023, 1179, 1705, 1923, 2839
AcuI CTGAAG 2 cut(s) 1629, 1839
AcyI GRCGYC 3 cut(s) 240, 915, 1689
AdeI CACNNNGTG 2 cut(s) 283, 492
AfaI GTAC 7 cut(s) 17, 585, 951, 1340, 2357, 2967, 3374
AfiI CCNNNNNNNGG 6 cut(s) 159, 1258, 2244, 2957, 3274, 3352
AflII CTTAAG 2 cut(s) 92, 2942
AflIII ACRYGT 2 cut(s) 2810, 3040
AgeI ACCGGT 1 cut(s) 232
AjnI CCWGG 5 cut(s) 353, 2865, 2905, 3156, 3195
AjuI GAANNNNNNNTTGG 6 cut(s) 2403, 2435, 3030, 3062, 3335, 3367
AloI GAACNNNNNNTCC 4 cut(s) 1511, 1543, 2739, 2771
Alw21I GWGCWC 1 cut(s) 739
Alw26I GTCTC 3 cut(s) 1286, 1497, 1964
AlwI GGATC 4 cut(s) 1120, 1133, 2055, 2917
Aor13HI TCCGGA 1 cut(s) 2638
AoxI GGCC 5 cut(s) 882, 1434, 1719, 1825, 1868
ApaI GGGCCC 1 cut(s) 1438
ApeKI GCWGC 6 cut(s) 674, 1472, 1596, 2547, 2989, 3435
ApoI RAATTY 9 cut(s) 369, 546, 631, 694, 1023, 1179, 1705, 1923, 2839
ArsI GACNNNNNNTTYG 2 cut(s) 3255, 3287
AsiGI ACCGGT 1 cut(s) 232
Asp700I GAANNNNTTC 2 cut(s) 113, 3360
AspA2I CCTAGG 1 cut(s) 266
AspLEI GCGC 3 cut(s) 868, 1400, 1697
AspS9I GGNCC 7 cut(s) 388, 1434, 1435, 1720, 1826, 2938, 3134
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 7 cut(s) 139, 620, 946, 1457, 1726, 1852, 2906
AsuII TTCGAA 2 cut(s) 1574, 2567
AvaII GGWCC 3 cut(s) 388, 2938, 3134
AvrII CCTAGG 1 cut(s) 266
BaeGI GKGCMC 1 cut(s) 1438
BaeI ACNNNNGTAYC 2 cut(s) 502, 535
BamHI GGATCC 1 cut(s) 1125
BanII GRGCYC 2 cut(s) 1090, 1438
BbsI GAAGAC 7 cut(s) 540, 929, 1076, 1397, 1827, 3252, 3326
Bbv12I GWGCWC 1 cut(s) 739
BbvCI CCTCAGC 1 cut(s) 1515
BbvI GCAGC 6 cut(s) 661, 1459, 1583, 2559, 3001, 3422
BccI CCATC 7 cut(s) 699, 1455, 2323, 2390, 2983, 3085, 3463
BceAI ACGGC 4 cut(s) 734, 1236, 1496, 1840
BciT130I CCWGG 5 cut(s) 355, 2867, 2907, 3158, 3197
BciVI GTATCC 2 cut(s) 1009, 3268
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 3 cut(s) 1286, 1497, 1964
BfmI CTRYAG 1 cut(s) 2917
BfrI CTTAAG 2 cut(s) 92, 2942
BfuI GTATCC 2 cut(s) 1009, 3268
BglII AGATCT 1 cut(s) 3358
BisI GCNGC 8 cut(s) 662, 675, 1473, 1597, 2056, 2548, 2990, 3436
BlnI CCTAGG 1 cut(s) 266
BlsI GCNGC 8 cut(s) 663, 676, 1474, 1598, 2057, 2549, 2991, 3437
Bme1390I CCNGG 6 cut(s) 355, 386, 2867, 2907, 3158, 3197
Bme18I GGWCC 3 cut(s) 388, 2938, 3134
BmgT120I GGNCC 7 cut(s) 388, 1434, 1435, 1720, 1826, 2938, 3134
BmiI GGNNCC 3 cut(s) 1127, 1436, 3488
BmrFI CCNGG 6 cut(s) 355, 386, 2867, 2907, 3158, 3197
BpiI GAAGAC 7 cut(s) 540, 929, 1076, 1397, 1827, 3252, 3326
BpmI CTGGAG 1 cut(s) 1946
Bpu10I CCTNAGC 1 cut(s) 1515
Bpu14I TTCGAA 2 cut(s) 1574, 2567
BpuEI CTTGAG 3 cut(s) 812, 1046, 2195
BpuMI CCSGG 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 1886
BsaHI GRCGYC 3 cut(s) 240, 915, 1689
BsaJI CCNNGG 6 cut(s) 266, 1257, 1829, 1871, 2731, 3196
BsaWI WCCGGW 2 cut(s) 232, 2638
Bsc4I CCNNNNNNNGG 6 cut(s) 159, 1258, 2244, 2957, 3274, 3352
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 5 cut(s) 13, 87, 2065, 2238, 2648
Bse3DI GCAATG 2 cut(s) 74, 2369
BseAI TCCGGA 1 cut(s) 2638
BseBI CCWGG 5 cut(s) 355, 2867, 2907, 3158, 3197
BseDI CCNNGG 6 cut(s) 266, 1257, 1829, 1871, 2731, 3196
BseLI CCNNNNNNNGG 6 cut(s) 159, 1258, 2244, 2957, 3274, 3352
BseMI GCAATG 2 cut(s) 74, 2369
BseMII CTCAG 6 cut(s) 1506, 2664, 3074, 3239, 3413, 3544
BseNI ACTGG 5 cut(s) 13, 87, 2065, 2238, 2648
BseRI GAGGAG 2 cut(s) 668, 3501
BseSI GKGCMC 1 cut(s) 1438
BseXI GCAGC 6 cut(s) 661, 1459, 1583, 2559, 3001, 3422
BseYI CCCAGC 1 cut(s) 1048
Bsh1236I CGCG 2 cut(s) 1695, 1831
BshFI GGCC 5 cut(s) 884, 1436, 1721, 1827, 1870
BshTI ACCGGT 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 739
BsiSI CCGG 3 cut(s) 233, 386, 2639
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 6 cut(s) 159, 1258, 2244, 2957, 3274, 3352
BsmAI GTCTC 3 cut(s) 1286, 1497, 1964
BsmBI CGTCTC 2 cut(s) 1286, 1497
BsmFI GGGAC 1 cut(s) 722
BsmI GAATGC 5 cut(s) 527, 868, 1400, 2806, 3269
BsnI GGCC 5 cut(s) 884, 1436, 1721, 1827, 1870
Bsp119I TTCGAA 2 cut(s) 1574, 2567
Bsp120I GGGCCC 1 cut(s) 1434
Bsp1286I GDGCHC 3 cut(s) 739, 1090, 1438
Bsp13I TCCGGA 1 cut(s) 2638
Bsp1407I TGTACA 3 cut(s) 949, 2355, 3372
Bsp143I GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
Bsp19I CCATGG 1 cut(s) 1257
BspACI CCGC 7 cut(s) 56, 661, 1693, 1712, 1829, 1831, 2056
BspANI GGCC 5 cut(s) 884, 1436, 1721, 1827, 1870
BspCNI CTCAG 6 cut(s) 1507, 2665, 3075, 3238, 3414, 3543
BspEI TCCGGA 1 cut(s) 2638
BspFNI CGCG 2 cut(s) 1695, 1831
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 1127, 1436, 3488
BspMAI CTGCAG 1 cut(s) 2921
BspPI GGATC 4 cut(s) 1120, 1133, 2055, 2917
BspQI GCTCTTC 1 cut(s) 590
BspT104I TTCGAA 2 cut(s) 1574, 2567
BspTI CTTAAG 2 cut(s) 92, 2942
BsrDI GCAATG 2 cut(s) 74, 2369
BsrFI RCCGGY 1 cut(s) 232
BsrGI TGTACA 3 cut(s) 949, 2355, 3372
BsrI ACTGG 5 cut(s) 13, 87, 2065, 2238, 2648
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 6 cut(s) 266, 1257, 1829, 1871, 2731, 3196
BssMI GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
BssNI GRCGYC 3 cut(s) 240, 915, 1689
BssT1I CCWWGG 2 cut(s) 266, 1257
Bst2UI CCWGG 5 cut(s) 355, 2867, 2907, 3158, 3197
Bst4CI ACNGT 9 cut(s) 64, 763, 2232, 2355, 2555, 2573, 2732, 2970, 3102
Bst6I CTCTTC 6 cut(s) 487, 590, 1148, 1935, 2841, 3212
BstACI GRCGYC 3 cut(s) 240, 915, 1689
BstAFI CTTAAG 2 cut(s) 92, 2942
BstAUI TGTACA 3 cut(s) 949, 2355, 3372
BstBAI YACGTR 1 cut(s) 1886
BstBI TTCGAA 2 cut(s) 1574, 2567
BstC8I GCNNGC 5 cut(s) 313, 1693, 1739, 1829, 1931
BstDSI CCRYGG 3 cut(s) 1257, 1829, 2731
BstFNI CGCG 2 cut(s) 1695, 1831
BstHHI GCGC 3 cut(s) 868, 1400, 1697
BstKTI GATC 8 cut(s) 853, 1128, 1198, 2050, 2597, 2912, 3073, 3361
BstMAI GTCTC 3 cut(s) 1286, 1497, 1964
BstMBI GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
BstMWI GCNNNNNNNGC 2 cut(s) 1421, 1718
BstNI CCWGG 5 cut(s) 355, 2867, 2907, 3158, 3197
BstNSI RCATGY 4 cut(s) 1684, 2362, 2814, 3044
BstSCI CCNGG 6 cut(s) 353, 384, 2865, 2905, 3156, 3195
BstSFI CTRYAG 1 cut(s) 2917
BstSLI GKGCMC 1 cut(s) 1438
BstUI CGCG 2 cut(s) 1695, 1831
BstV1I GCAGC 6 cut(s) 661, 1459, 1583, 2559, 3001, 3422
BstV2I GAAGAC 7 cut(s) 540, 929, 1076, 1397, 1827, 3252, 3326
BstX2I RGATCY 4 cut(s) 1125, 2047, 2909, 3358
BstXI CCANNNNNNTGG 1 cut(s) 1240
BstYI RGATCY 4 cut(s) 1125, 2047, 2909, 3358
BsuI GTATCC 2 cut(s) 1009, 3268
BsuRI GGCC 5 cut(s) 884, 1436, 1721, 1827, 1870
BtgI CCRYGG 3 cut(s) 1257, 1829, 2731
BtgZI GCGATG 1 cut(s) 44
BtsI GCAGTG 1 cut(s) 671
BtsIMutI CAGTG 3 cut(s) 671, 2058, 3233
Cac8I GCNNGC 5 cut(s) 313, 1693, 1739, 1829, 1931
CciI TCATGA 1 cut(s) 858
CfoI GCGC 3 cut(s) 868, 1400, 1697
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 7 cut(s) 388, 1434, 1435, 1720, 1826, 2938, 3134
Cfr42I CCGCGG 1 cut(s) 1832
CseI GACGC 4 cut(s) 317, 904, 1479, 1697
Csp6I GTAC 7 cut(s) 16, 584, 950, 1339, 2356, 2966, 3373
CspAI ACCGGT 1 cut(s) 232
CspCI CAANNNNNGTGG 4 cut(s) 874, 909, 3015, 3050
CviQI GTAC 7 cut(s) 16, 584, 950, 1339, 2356, 2966, 3373
DpnI GATC 8 cut(s) 852, 1127, 1197, 2049, 2596, 2911, 3072, 3360
DpnII GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
DraI TTTAAA 1 cut(s) 681
DraIII CACNNNGTG 2 cut(s) 283, 492
DrdI GACNNNNNNGTC 1 cut(s) 2648
DseDI GACNNNNNNGTC 1 cut(s) 2648
EaeI YGGCCR 1 cut(s) 1868
Eam1104I CTCTTC 6 cut(s) 487, 590, 1148, 1935, 2841, 3212
EarI CTCTTC 6 cut(s) 487, 590, 1148, 1935, 2841, 3212
Eco130I CCWWGG 2 cut(s) 266, 1257
Eco24I GRGCYC 2 cut(s) 1090, 1438
Eco32I GATATC 6 cut(s) 590, 1621, 1809, 2002, 2257, 2328
Eco47I GGWCC 3 cut(s) 388, 2938, 3134
Eco57I CTGAAG 2 cut(s) 1629, 1839
EcoO109I RGGNCCY 4 cut(s) 1434, 1435, 1720, 2938
EcoRI GAATTC 2 cut(s) 546, 1179
EcoRII CCWGG 5 cut(s) 353, 2865, 2905, 3156, 3195
EcoRV GATATC 6 cut(s) 590, 1621, 1809, 2002, 2257, 2328
EcoT14I CCWWGG 2 cut(s) 266, 1257
EcoT22I ATGCAT 3 cut(s) 527, 1538, 1779
EcoT38I GRGCYC 2 cut(s) 1090, 1438
ErhI CCWWGG 2 cut(s) 266, 1257
Esp3I CGTCTC 2 cut(s) 1286, 1497
FaqI GGGAC 1 cut(s) 722
FauI CCCGC 2 cut(s) 1700, 1836
FauNDI CATATG 1 cut(s) 3125
FbaI TGATCA 1 cut(s) 850
FblI GTMKAC 2 cut(s) 306, 900
Fnu4HI GCNGC 8 cut(s) 662, 675, 1473, 1597, 2056, 2548, 2990, 3436
FriOI GRGCYC 2 cut(s) 1090, 1438
Fsp4HI GCNGC 8 cut(s) 662, 675, 1473, 1597, 2056, 2548, 2990, 3436
GlaI GCGC 3 cut(s) 867, 1399, 1696
GluI GCNGC 8 cut(s) 662, 675, 1473, 1597, 2056, 2548, 2990, 3436
GsaI CCCAGC 1 cut(s) 1052
GsuI CTGGAG 1 cut(s) 1946
HaeIII GGCC 5 cut(s) 884, 1436, 1721, 1827, 1870
HapII CCGG 3 cut(s) 233, 386, 2639
HgaI GACGC 4 cut(s) 317, 904, 1479, 1697
HhaI GCGC 3 cut(s) 868, 1400, 1697
Hin1I GRCGYC 3 cut(s) 240, 915, 1689
Hin6I GCGC 3 cut(s) 866, 1398, 1695
HinP1I GCGC 3 cut(s) 866, 1398, 1695
HincII GTYRAC 3 cut(s) 238, 2235, 2653
HindII GTYRAC 3 cut(s) 238, 2235, 2653
HindIII AAGCTT 2 cut(s) 2109, 2171
HpaII CCGG 3 cut(s) 233, 386, 2639
HphI GGTGA 7 cut(s) 139, 620, 946, 1457, 1726, 1852, 2906
Hpy166II GTNNAC 7 cut(s) 238, 307, 901, 950, 2235, 2527, 2653
Hpy8I GTNNAC 7 cut(s) 238, 307, 901, 950, 2235, 2527, 2653
Hpy99I CGWCG 3 cut(s) 245, 920, 1482
HpyCH4III ACNGT 9 cut(s) 64, 763, 2232, 2355, 2555, 2573, 2732, 2970, 3102
HpyCH4IV ACGT 6 cut(s) 18, 144, 240, 1279, 1761, 1885
HpyF10VI GCNNNNNNNGC 2 cut(s) 1421, 1718
HpySE526I ACGT 6 cut(s) 18, 144, 240, 1279, 1761, 1885
Hsp92I GRCGYC 3 cut(s) 240, 915, 1689
HspAI GCGC 3 cut(s) 866, 1398, 1695
Kpn2I TCCGGA 1 cut(s) 2638
Ksp22I TGATCA 1 cut(s) 850
KspI CCGCGG 1 cut(s) 1832
Kzo9I GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
LguI GCTCTTC 1 cut(s) 590
LmnI GCTCC 4 cut(s) 268, 1311, 3145, 3514
Lsp1109I GCAGC 6 cut(s) 661, 1459, 1583, 2559, 3001, 3422
MaeII ACGT 6 cut(s) 18, 144, 240, 1279, 1761, 1885
MaeIII GTNAC 8 cut(s) 58, 133, 185, 2690, 2708, 2739, 2898, 3115
MalI GATC 8 cut(s) 852, 1127, 1197, 2049, 2596, 2911, 3072, 3360
MboI GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
MfeI CAATTG 2 cut(s) 1626, 3239
MflI RGATCY 4 cut(s) 1125, 2047, 2909, 3358
MhlI GDGCHC 3 cut(s) 739, 1090, 1438
MlyI GAGTC 6 cut(s) 906, 1724, 1742, 2049, 3032, 3433
Mph1103I ATGCAT 3 cut(s) 527, 1538, 1779
MroI TCCGGA 1 cut(s) 2638
MroXI GAANNNNTTC 2 cut(s) 113, 3360
MslI CAYNNNNRTG 2 cut(s) 122, 2717
MspA1I CMGCKG 2 cut(s) 1712, 1831
MspCI CTTAAG 2 cut(s) 92, 2942
MspI CCGG 3 cut(s) 233, 386, 2639
MspR9I CCNGG 6 cut(s) 355, 386, 2867, 2907, 3158, 3197
MunI CAATTG 2 cut(s) 1626, 3239
Mva1269I GAATGC 5 cut(s) 527, 868, 1400, 2806, 3269
MvaI CCWGG 5 cut(s) 355, 2867, 2907, 3158, 3197
MvnI CGCG 2 cut(s) 1695, 1831
MwoI GCNNNNNNNGC 2 cut(s) 1421, 1718
NciI CCSGG 1 cut(s) 386
NcoI CCATGG 1 cut(s) 1257
NdeI CATATG 1 cut(s) 3125
NdeII GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
NlaIV GGNNCC 3 cut(s) 1127, 1436, 3488
NmeAIII GCCGAG 1 cut(s) 1896
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 3 cut(s) 527, 1538, 1779
NspI RCATGY 4 cut(s) 1684, 2362, 2814, 3044
NspV TTCGAA 2 cut(s) 1574, 2567
PagI TCATGA 1 cut(s) 858
PciI ACATGT 2 cut(s) 2810, 3040
PciSI GCTCTTC 1 cut(s) 590
PctI GAATGC 5 cut(s) 527, 868, 1400, 2806, 3269
PdmI GAANNNNTTC 2 cut(s) 113, 3360
PfeI GAWTC 8 cut(s) 440, 571, 1304, 1673, 2179, 2274, 2631, 3552
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 8 cut(s) 663, 676, 1474, 1598, 2057, 2549, 2991, 3437
PleI GAGTC 6 cut(s) 905, 1724, 1742, 2048, 3031, 3432
PpsI GAGTC 6 cut(s) 905, 1724, 1742, 2048, 3031, 3432
Ppu21I YACGTR 1 cut(s) 1886
PpuMI RGGWCCY 1 cut(s) 2938
PscI ACATGT 2 cut(s) 2810, 3040
PsiI TTATAA 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 2938
Psp6I CCWGG 5 cut(s) 353, 2865, 2905, 3156, 3195
PspFI CCCAGC 1 cut(s) 1048
PspGI CCWGG 5 cut(s) 353, 2865, 2905, 3156, 3195
PspN4I GGNNCC 3 cut(s) 1127, 1436, 3488
PspOMI GGGCCC 1 cut(s) 1434
PspPI GGNCC 7 cut(s) 388, 1434, 1435, 1720, 1826, 2938, 3134
PspPPI RGGWCCY 1 cut(s) 2938
PsrI GAACNNNNNNTAC 2 cut(s) 2605, 2637
PstI CTGCAG 1 cut(s) 2921
PsuI RGATCY 4 cut(s) 1125, 2047, 2909, 3358
RsaI GTAC 7 cut(s) 17, 585, 951, 1340, 2357, 2967, 3374
RsaNI GTAC 7 cut(s) 16, 584, 950, 1339, 2356, 2966, 3373
RseI CAYNNNNRTG 2 cut(s) 122, 2717
SacII CCGCGG 1 cut(s) 1832
SapI GCTCTTC 1 cut(s) 590
SatI GCNGC 8 cut(s) 662, 675, 1473, 1597, 2056, 2548, 2990, 3436
Sau3AI GATC 8 cut(s) 850, 1125, 1195, 2047, 2594, 2909, 3070, 3358
Sau96I GGNCC 7 cut(s) 388, 1434, 1435, 1720, 1826, 2938, 3134
SchI GAGTC 6 cut(s) 906, 1724, 1742, 2049, 3032, 3433
ScrFI CCNGG 6 cut(s) 355, 386, 2867, 2907, 3158, 3197
SduI GDGCHC 3 cut(s) 739, 1090, 1438
SfcI CTRYAG 1 cut(s) 2917
Sfr303I CCGCGG 1 cut(s) 1832
SfuI TTCGAA 2 cut(s) 1574, 2567
SgrBI CCGCGG 1 cut(s) 1832
SinI GGWCC 3 cut(s) 388, 2938, 3134
SmiMI CAYNNNNRTG 2 cut(s) 122, 2717
SmlI CTYRAG 5 cut(s) 92, 791, 1061, 2174, 2942
SmoI CTYRAG 5 cut(s) 92, 791, 1061, 2174, 2942
SsiI CCGC 7 cut(s) 56, 661, 1693, 1712, 1829, 1831, 2056
SspI AATATT 2 cut(s) 1803, 2419
StyD4I CCNGG 6 cut(s) 353, 384, 2865, 2905, 3156, 3195
StyI CCWWGG 2 cut(s) 266, 1257
TaaI ACNGT 9 cut(s) 64, 763, 2232, 2355, 2555, 2573, 2732, 2970, 3102
TaiI ACGT 6 cut(s) 21, 147, 243, 1282, 1764, 1888
TaqI TCGA 7 cut(s) 243, 343, 1441, 1477, 1574, 2511, 2567
TatI WGTACW 4 cut(s) 949, 2355, 2965, 3372
TauI GCSGC 2 cut(s) 664, 2058
TfiI GAWTC 8 cut(s) 440, 571, 1304, 1673, 2179, 2274, 2631, 3552
TscAI CASTG 3 cut(s) 671, 2065, 3233
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 6 cut(s) 674, 1472, 1596, 2547, 2989, 3435
Tsp45I GTSAC 1 cut(s) 133
TspGWI ACGGA 2 cut(s) 660, 918
TspRI CASTG 3 cut(s) 671, 2065, 3233
Vha464I CTTAAG 2 cut(s) 92, 2942
VpaK11BI GGWCC 3 cut(s) 388, 2938, 3134
XapI RAATTY 9 cut(s) 369, 546, 631, 694, 1023, 1179, 1705, 1923, 2839
XbaI TCTAGA 3 cut(s) 1751, 2818, 3106
XceI RCATGY 4 cut(s) 1684, 2362, 2814, 3044
XcmI CCANNNNNNNNNTGG 2 cut(s) 493, 1237
XmaJI CCTAGG 1 cut(s) 266
XmiI GTMKAC 2 cut(s) 306, 900
XmnI GAANNNNTTC 2 cut(s) 113, 3360
ZraI GACGTC 1 cut(s) 241
Zsp2I ATGCAT 3 cut(s) 527, 1538, 1779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.