RLG00000035464

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000085
Physical Location & Seq
Reverse (-)
67221808 .. 67226363
4556 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035464

Sequence Viewer

Length: 2799 bp
ATGGAAGACACTCGCAGGGGTTTTGTTGTCCATTCAGACCATGAATTGCAAAACAGAGGACTATGTAAAACATTCAAGGATGAAGACCAACTTGAAAAAGGGGTAGCCCTCCCCTCTGCATCTCTCTCCTCAACTAAACCAAGTCCTCAACTAGCTCTTGCGCAGAAGAATGACGAGATTTTGGGTTTAAGGGGAAACGACACTCAAAGAGGTTCTGTCACCCATTCAGGTCATGAAATACAAAACACCAGAGTGACTAAGACATTCACCGATGAAGAACATCTTGAAAAAGGGATAGCTGTCCCCTCTACGTCTCCGCCTTCATCAGCTGAACCAGCTCCTCGACCGAAGTATGATGTGTTTTTGAGTTTCAGCGGGGAAGACAGTCGTAGGGGTTTTGTAGCTCATTTATACCGTGAATTGCAAAATACCAGAGTGATTAAAACTTTCAAGGATGACGTGCAACTACAAAAAGGGACGGTTATTTCTTCAAATCTCGAAATGGCAATCGACAAATCAAGGTTTGCGGTCGTTGTGTTGTCGGAGAACTATGCTTCTTCTGCATGGTGTTTGAATGAACTTACAAAGATTTATCAATGCATGGAGGGTAAGGACACAATTCTCCCAATTTTCTATAATTTGGATCCCGCTGTTGTACGATATCAAAACAAAGGGACAAGTTTTGGAAAAGCTTTCATTAAGCATGAAGCTAGACAAGACATTGAGAAGATGAACCAGTGGAGAGCTGCTTTAAAAGGAGTGGCTGGAATCGCTGGGTGGCATTCAAACAACTATCCGACGGATATAGAACTAATTGAAGACATTGTGAAACATTTGTGGGAGAAAGTACTTCAAGCACCCACCGGAAATTTTGAAACATTTGAAGCGACAAGGCAAGCCATGGATGAGATCATGAAGGTATTAAAAGACGATCAGGTCACTGCCCTTGGGTTGCATGGAGTGGGTGGCGTTGGAAAGACGACGTTGGTCAAACATGTTAGTGCAGAAGCCCAGAAAGATGGGCTTTTTGATCACGTGATTACGGTTGTCGTATCCAAAAGCCCTGACTTGCAAGAAATTCAAGGCACATTGGCAGATGCATTGAGCTTGGAGGAGGGGATCATGAGAAGAAATGGGATGCTTATAATATTGGATGACATTTGGGAGAGTTTTGAACTCTCGAGCATTGGAATTCCCAGTCACAATGAGCTTCAAAGGTGCAATTCTAAAGTTATACTCACCACAAGGAAAAGCAATGTCTGCCATTCTATGGGGTGCCATGCCCAGATCCCTCTCAATGTTCTATCAGAGGAAGATTCTTGGAAGCTGTTCATGGAGATGTCGGGGAAGTCTTTTCATAAATCTACCGTTTCCTATGATGAAGCGAGAAAGGTAGCTGGAGAATGCGGTGGTCTCCCAAAAGCAATAAAAGCGGCTGCAAGGTCACTTGGAGATAAATACTTGGACGAATGGAATGCAGCAGTTGCAACAGCATTTGGAGATGACTGCTTGGACGAATGGAATGAAACGGCTCAATTTGTCAACTCCGAAGCTGAGGAAGACGCATTTCATTCATTGGCATCTGATGATGCCAAGTCATGCTTCTTGCTATGCTGCCTATTCCCAGAAAATTATGATATCCCAATTGAAGAGTTGCTCAAGTACGGGATTGGAATAGGTTTGTTTCAATATGATACAATGCAAGAAGCCAGAGAGAGAGCACATTCGGTGGTCAAGTACCTCAAAGCTTCTAGACTGCTTTTGGACGGTACAAAGAAGGGATGCATAAGGATGCCTGATGACATTCGAAATCTGGCCATGTCCATTGTGCGGTCCGAAGAAGGTGATGGGTTTTTAGTAAAAGCTGGTTGTGAACTAGAGGAGTGGCCAGAGGCAGAGACAGAGGCAGGATGGCTGCAAGGATTTTGCTTAACAATTTCTATGTCATTTGTGGCTAACAATTTCGCACCCCTAGGAATTACTTTCCGGCTCCGCAAAATCTGGGATTGGCCAATAGATGCTCATGAAGGCTACTCAATAATCTCACTCATGAAAAACAACCTTCATAAGCTTCCCGAACATTTGGTATGTTCAGAGCTCCAAATTTTATTGTTACAAAATAATGCTGGTATACGTGACATCCCAGAAACCTTTCTTCAAAGTCCTAATGCTCTAAGGGTCTTTGATCTTAGCTTCACTAGCATTTCATCACTACCTTCATCATTTAGTTTCCTAACCAACCTTCAAGTTTTGAAATTGGATTTTTGCCATAATTTAAGTAACATATCCGTTCTAGGAAAACTTAAGAAACTAGAGATTCTTAGTATGAGAGAACTTCCTCTTAAGAAATTTCCGACACAAATAGGAGATTTGACCAGTCTAAGGGTGTTGGACGTCACTGGTAGATATCTTGAAACAATCCCATCTAAAGTGATATCGAGGTTGTACAGATTGGAAGAATTGCACATGAAATTTCAATTTGGGAAGTGGGGAAGTAAAATTGAGGATGTAGTTGAAGAAACCAATGCTGACTTTGATGAGTTGACTGGCTTGTTGGATTTAAACATTTTGGAAGTTTACATATATGATGCTGCATGCTTGCCTGAAAGTATTGAGTACTTCAAGCCGACTTGGGTAAACTTTGATATATGCATCAGCAGACACGTCCCTTACATGCTGCTCCCTCAACATGATGGTGGGAGGAAGTGCATAATCGGCTATAAGAGAGCTGCTGCTGCACTAGGGAAAAAAGAAAAAAAAGAGATGTTCAATGGTCCCAGAACAAGATTCCTCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

933

Amino Acids

104.64

Weight (kDa)

5.6

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 117 - 288 9.4e-48 TIR domain
TIR_2 PF13676 120 - 216 1.8e-14 TIR domain
NB-ARC PF00931 299 - 448 1.2e-26 NB-ARC domain
LRR_8 PF13855 699 - 757 1.5e-07 Leucine rich repeat
LRR_14 PF23598 728 - 824 5.4e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1145
AasI GACNNNNNNGTC 1 cut(s) 935
AatII GACGTC 1 cut(s) 2397
Acc16I TGCGCA 1 cut(s) 162
AccB1I GGYRCC 1 cut(s) 1275
AccB7I CCANNNNNTGG 1 cut(s) 1270
AccI GTMKAC 1 cut(s) 2131
AciI CCGC 8 cut(s) 317, 375, 527, 648, 1407, 1433, 1831, 1993
AclWI GGATC 4 cut(s) 638, 651, 1127, 1282
AcoI YGGCCR 3 cut(s) 1815, 1886, 2009
AcsI RAATTY 6 cut(s) 868, 1077, 1191, 2103, 2348, 2471
AcvI CACGTG 1 cut(s) 1036
AcyI GRCGYC 1 cut(s) 2394
AfaI GTAC 7 cut(s) 657, 849, 1664, 1739, 1771, 2447, 2618
AfiI CCNNNNNNNGG 3 cut(s) 1270, 1830, 2382
AflII CTTAAG 2 cut(s) 2303, 2342
AflIII ACRYGT 2 cut(s) 994, 2662
AjiI CACGTC 2 cut(s) 460, 2665
AjuI GAANNNNNNNTTGG 2 cut(s) 1586, 1618
AleI CACNNNNGTG 1 cut(s) 251
Alw21I GWGCWC 2 cut(s) 1723, 2100
Alw26I GTCTC 3 cut(s) 318, 1418, 1892
AlwI GGATC 4 cut(s) 638, 651, 1127, 1282
Ama87I CYCGRG 1 cut(s) 1180
AoxI GGCC 3 cut(s) 1815, 1886, 2009
ApoI RAATTY 6 cut(s) 868, 1077, 1191, 2103, 2348, 2471
Asp700I GAANNNNTTC 2 cut(s) 1328, 2151
AspA2I CCTAGG 1 cut(s) 1972
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 1833, 2774
AsuHPI GGTGA 4 cut(s) 211, 259, 1231, 1856
AsuII TTCGAA 1 cut(s) 1807
AvaI CYCGRG 1 cut(s) 1180
AvaII GGWCC 2 cut(s) 1833, 2774
AvrII CCTAGG 1 cut(s) 1972
BalI TGGCCA 3 cut(s) 1817, 1888, 2011
BamHI GGATCC 1 cut(s) 643
BanI GGYRCC 1 cut(s) 1275
BanII GRGCYC 1 cut(s) 2100
BbrPI CACGTG 1 cut(s) 1036
BbsI GAAGAC 5 cut(s) 12, 90, 387, 825, 1566
Bbv12I GWGCWC 2 cut(s) 1723, 2100
BbvCI CCTCAGC 1 cut(s) 1554
BccI CCATC 5 cut(s) 1013, 1841, 1905, 2431, 2687
BceAI ACGGC 1 cut(s) 1545
BcgI CGANNNNNNTGC 2 cut(s) 1457, 1491
BciVI GTATCC 1 cut(s) 1063
BclI TGATCA 1 cut(s) 1030
BcoDI GTCTC 3 cut(s) 318, 1418, 1892
BfaI CTAG 9 cut(s) 152, 711, 1752, 1877, 1973, 2199, 2294, 2312, 2741
BfrI CTTAAG 2 cut(s) 2303, 2342
BfuI GTATCC 1 cut(s) 1063
BlnI CCTAGG 1 cut(s) 1972
BmcAI AGTACT 2 cut(s) 849, 2618
Bme18I GGWCC 2 cut(s) 1833, 2774
BmeT110I CYCGRG 1 cut(s) 1180
BmgBI CACGTC 2 cut(s) 460, 2665
BmgT120I GGNCC 2 cut(s) 1833, 2774
BmiI GGNNCC 4 cut(s) 645, 1277, 1991, 2776
BmrI ACTGGG 1 cut(s) 1191
BmuI ACTGGG 1 cut(s) 1191
BoxI GACNNNNGTC 1 cut(s) 986
BpiI GAAGAC 5 cut(s) 12, 90, 387, 825, 1566
BpmI CTGGAG 1 cut(s) 1419
Bpu10I CCTNAGC 1 cut(s) 1554
Bpu14I TTCGAA 1 cut(s) 1807
BpuEI CTTGAG 1 cut(s) 1643
BsaAI YACGTR 2 cut(s) 1036, 2135
BsaHI GRCGYC 1 cut(s) 2394
BsaI GGTCTC 1 cut(s) 1418
BsaJI CCNNGG 3 cut(s) 900, 946, 1972
BsaWI WCCGGW 1 cut(s) 863
Bsc4I CCNNNNNNNGG 3 cut(s) 1270, 1830, 2382
Bse1I ACTGG 5 cut(s) 736, 1197, 2376, 2404, 2551
Bse3DI GCAATG 1 cut(s) 1261
BseDI CCNNGG 3 cut(s) 900, 946, 1972
BseLI CCNNNNNNNGG 3 cut(s) 1270, 1830, 2382
BseMI GCAATG 1 cut(s) 1261
BseMII CTCAG 1 cut(s) 1545
BseNI ACTGG 5 cut(s) 736, 1197, 2376, 2404, 2551
BseRI GAGGAG 4 cut(s) 118, 330, 1127, 1895
BseYI CCCAGC 1 cut(s) 773
BsgI GTGCAG 2 cut(s) 1023, 2721
Bsh1285I CGRYCG 2 cut(s) 347, 531
BshFI GGCC 3 cut(s) 1817, 1888, 2011
BshNI GGYRCC 1 cut(s) 1275
BsiEI CGRYCG 2 cut(s) 347, 531
BsiHKAI GWGCWC 2 cut(s) 1723, 2100
BsiHKCI CYCGRG 1 cut(s) 1180
BsiSI CCGG 2 cut(s) 864, 1987
BslFI GGGAC 5 cut(s) 287, 490, 688, 2651, 2760
BslI CCNNNNNNNGG 3 cut(s) 1270, 1830, 2382
BsmAI GTCTC 3 cut(s) 318, 1418, 1892
BsmBI CGTCTC 1 cut(s) 318
BsmFI GGGAC 5 cut(s) 287, 490, 688, 2651, 2760
BsmI GAATGC 3 cut(s) 781, 1409, 1480
BsnI GGCC 3 cut(s) 1817, 1888, 2011
Bso31I GGTCTC 1 cut(s) 1418
BsoBI CYCGRG 1 cut(s) 1180
Bsp119I TTCGAA 1 cut(s) 1807
Bsp1286I GDGCHC 2 cut(s) 1723, 2100
Bsp1407I TGTACA 1 cut(s) 2445
Bsp143I GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 8 cut(s) 317, 375, 527, 648, 1407, 1433, 1831, 1993
BspANI GGCC 3 cut(s) 1817, 1888, 2011
BspCNI CTCAG 1 cut(s) 1546
BspHI TCATGA 5 cut(s) 232, 912, 1122, 2023, 2049
BspLI GGNNCC 4 cut(s) 645, 1277, 1991, 2776
BspPI GGATC 4 cut(s) 638, 651, 1127, 1282
BspT104I TTCGAA 1 cut(s) 1807
BspT107I GGYRCC 1 cut(s) 1275
BspTI CTTAAG 2 cut(s) 2303, 2342
BspTNI GGTCTC 1 cut(s) 1418
BsrDI GCAATG 1 cut(s) 1261
BsrGI TGTACA 1 cut(s) 2445
BsrI ACTGG 5 cut(s) 736, 1197, 2376, 2404, 2551
BssECI CCNNGG 3 cut(s) 900, 946, 1972
BssMI GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
BssNAI GTATAC 1 cut(s) 2132
BssNI GRCGYC 1 cut(s) 2394
BssT1I CCWWGG 3 cut(s) 900, 946, 1972
Bst1107I GTATAC 1 cut(s) 2132
Bst4CI ACNGT 6 cut(s) 386, 416, 481, 1045, 1369, 1769
Bst6I CTCTTC 1 cut(s) 1644
BstACI GRCGYC 1 cut(s) 2394
BstAFI CTTAAG 2 cut(s) 2303, 2342
BstAPI GCANNNNNTGC 2 cut(s) 1260, 1484
BstAUI TGTACA 1 cut(s) 2445
BstBAI YACGTR 2 cut(s) 1036, 2135
BstBI TTCGAA 1 cut(s) 1807
BstC8I GCNNGC 3 cut(s) 897, 2596, 2600
BstDEI CTNAG 7 cut(s) 258, 1554, 2174, 2189, 2321, 2381, 2792
BstDSI CCRYGG 1 cut(s) 900
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 7 cut(s) 646, 912, 934, 1033, 1122, 1290, 2188
BstMAI GTCTC 3 cut(s) 318, 1418, 1892
BstMBI GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
BstMCI CGRYCG 2 cut(s) 347, 531
BstMWI GCNNNNNNNGC 9 cut(s) 335, 560, 770, 1260, 1430, 1484, 2199, 2715, 2735
BstNSI RCATGY 3 cut(s) 998, 2598, 2677
BstPAI GACNNNNGTC 1 cut(s) 986
BstV2I GAAGAC 5 cut(s) 12, 90, 387, 825, 1566
BstX2I RGATCY 2 cut(s) 643, 1287
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 2 cut(s) 643, 1287
BstZ17I GTATAC 1 cut(s) 2132
BsuI GTATCC 1 cut(s) 1063
BsuRI GGCC 3 cut(s) 1817, 1888, 2011
BtgI CCRYGG 1 cut(s) 900
BtrI CACGTC 2 cut(s) 460, 2665
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 3 cut(s) 743, 939, 2397
Cac8I GCNNGC 3 cut(s) 897, 2596, 2600
CciI TCATGA 5 cut(s) 232, 912, 1122, 2023, 2049
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 2 cut(s) 1833, 2774
CpoI CGGWCCG 1 cut(s) 1833
CseI GACGC 1 cut(s) 1571
Csp6I GTAC 7 cut(s) 656, 848, 1663, 1738, 1770, 2446, 2617
CspI CGGWCCG 1 cut(s) 1833
CviQI GTAC 7 cut(s) 656, 848, 1663, 1738, 1770, 2446, 2617
DdeI CTNAG 7 cut(s) 258, 1554, 2174, 2189, 2321, 2381, 2792
DpnI GATC 7 cut(s) 645, 911, 933, 1032, 1121, 1289, 2187
DpnII GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
DraI TTTAAA 2 cut(s) 753, 2562
DrdI GACNNNNNNGTC 1 cut(s) 935
DseDI GACNNNNNNGTC 1 cut(s) 935
EaeI YGGCCR 3 cut(s) 1815, 1886, 2009
Eam1104I CTCTTC 1 cut(s) 1644
EarI CTCTTC 1 cut(s) 1644
EciI GGCGGA 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 2098
Eco130I CCWWGG 3 cut(s) 900, 946, 1972
Eco24I GRGCYC 1 cut(s) 2100
Eco31I GGTCTC 1 cut(s) 1418
Eco32I GATATC 4 cut(s) 662, 1639, 2408, 2436
Eco47I GGWCC 2 cut(s) 1833, 2774
Eco53kI GAGCTC 1 cut(s) 2098
Eco72I CACGTG 1 cut(s) 1036
Eco88I CYCGRG 1 cut(s) 1180
EcoICRI GAGCTC 1 cut(s) 2098
EcoRI GAATTC 1 cut(s) 1191
EcoRV GATATC 4 cut(s) 662, 1639, 2408, 2436
EcoT14I CCWWGG 3 cut(s) 900, 946, 1972
EcoT22I ATGCAT 4 cut(s) 602, 1102, 1787, 2654
EcoT38I GRGCYC 1 cut(s) 2100
ErhI CCWWGG 3 cut(s) 900, 946, 1972
Esp3I CGTCTC 1 cut(s) 318
FalI AAGNNNNNCTT 8 cut(s) 75, 107, 267, 299, 1008, 1040, 1586, 1618
FaqI GGGAC 5 cut(s) 287, 490, 688, 2651, 2760
FauI CCCGC 2 cut(s) 368, 655
FbaI TGATCA 1 cut(s) 1030
FblI GTMKAC 1 cut(s) 2131
FriOI GRGCYC 1 cut(s) 2100
FspBI CTAG 9 cut(s) 152, 711, 1752, 1877, 1973, 2199, 2294, 2312, 2741
FspI TGCGCA 1 cut(s) 162
GlaI GCGC 1 cut(s) 162
GsaI CCCAGC 1 cut(s) 777
GsuI CTGGAG 1 cut(s) 1419
HaeIII GGCC 3 cut(s) 1817, 1888, 2011
HapII CCGG 2 cut(s) 864, 1987
HgaI GACGC 1 cut(s) 1571
HhaI GCGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 2394
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HincII GTYRAC 2 cut(s) 1543, 2544
HindII GTYRAC 2 cut(s) 1543, 2544
HindIII AAGCTT 3 cut(s) 690, 1746, 2069
HinfI GANTC 4 cut(s) 768, 1316, 2317, 2787
HpaII CCGG 2 cut(s) 864, 1987
HphI GGTGA 4 cut(s) 211, 259, 1231, 1856
Hpy166II GTNNAC 6 cut(s) 1543, 1874, 2132, 2544, 2578, 2638
Hpy188I TCNGA 9 cut(s) 37, 544, 798, 1309, 1549, 1585, 1837, 2095, 2355
Hpy8I GTNNAC 6 cut(s) 1543, 1874, 2132, 2544, 2578, 2638
Hpy99I CGWCG 2 cut(s) 802, 985
HpyAV CCTTC 8 cut(s) 330, 910, 1771, 1835, 2021, 2072, 2226, 2252
HpyCH4III ACNGT 6 cut(s) 386, 416, 481, 1045, 1369, 1769
HpyCH4IV ACGT 7 cut(s) 311, 459, 983, 1035, 2134, 2394, 2664
HpyF10VI GCNNNNNNNGC 9 cut(s) 335, 560, 770, 1260, 1430, 1484, 2199, 2715, 2735
HpyF3I CTNAG 7 cut(s) 258, 1554, 2174, 2189, 2321, 2381, 2792
HpySE526I ACGT 7 cut(s) 311, 459, 983, 1035, 2134, 2394, 2664
Hsp92I GRCGYC 1 cut(s) 2394
HspAI GCGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 1030
Kzo9I GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
LmnI GCTCC 4 cut(s) 343, 1995, 2103, 2685
MaeI CTAG 9 cut(s) 152, 711, 1752, 1877, 1973, 2199, 2294, 2312, 2741
MaeII ACGT 7 cut(s) 311, 459, 983, 1035, 2134, 2394, 2664
MaeIII GTNAC 9 cut(s) 217, 253, 937, 1199, 1443, 2112, 2135, 2279, 2395
MalI GATC 7 cut(s) 645, 911, 933, 1032, 1121, 1289, 2187
MboI GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
MfeI CAATTG 1 cut(s) 1644
MflI RGATCY 2 cut(s) 643, 1287
MhlI GDGCHC 2 cut(s) 1723, 2100
MlsI TGGCCA 3 cut(s) 1817, 1888, 2011
MluNI TGGCCA 3 cut(s) 1817, 1888, 2011
MmeI TCCRAC 6 cut(s) 522, 821, 952, 2370, 2378, 2535
Mox20I TGGCCA 3 cut(s) 1817, 1888, 2011
Mph1103I ATGCAT 4 cut(s) 602, 1102, 1787, 2654
MroXI GAANNNNTTC 2 cut(s) 1328, 2151
MscI TGGCCA 3 cut(s) 1817, 1888, 2011
MslI CAYNNNNRTG 5 cut(s) 251, 999, 1337, 1790, 2694
Msp20I TGGCCA 3 cut(s) 1817, 1888, 2011
MspA1I CMGCKG 3 cut(s) 329, 375, 650
MspCI CTTAAG 2 cut(s) 2303, 2342
MspI CCGG 2 cut(s) 864, 1987
MunI CAATTG 1 cut(s) 1644
Mva1269I GAATGC 3 cut(s) 781, 1409, 1480
MwoI GCNNNNNNNGC 9 cut(s) 335, 560, 770, 1260, 1430, 1484, 2199, 2715, 2735
NcoI CCATGG 1 cut(s) 900
NdeII GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
NlaIV GGNNCC 4 cut(s) 645, 1277, 1991, 2776
NmuCI GTSAC 7 cut(s) 217, 253, 937, 1199, 1443, 2135, 2395
NsbI TGCGCA 1 cut(s) 162
NsiI ATGCAT 4 cut(s) 602, 1102, 1787, 2654
NspI RCATGY 3 cut(s) 998, 2598, 2677
NspV TTCGAA 1 cut(s) 1807
OliI CACNNNNGTG 1 cut(s) 251
PaeI GCATGC 1 cut(s) 2598
PaeR7I CTCGAG 1 cut(s) 1180
PagI TCATGA 5 cut(s) 232, 912, 1122, 2023, 2049
PciI ACATGT 1 cut(s) 994
PctI GAATGC 3 cut(s) 781, 1409, 1480
PdmI GAANNNNTTC 2 cut(s) 1328, 2151
PfeI GAWTC 4 cut(s) 768, 1316, 2317, 2787
PflMI CCANNNNNTGG 1 cut(s) 1270
PmaCI CACGTG 1 cut(s) 1036
PmlI CACGTG 1 cut(s) 1036
Ppu21I YACGTR 2 cut(s) 1036, 2135
PscI ACATGT 1 cut(s) 994
PshAI GACNNNNGTC 1 cut(s) 986
PsiI TTATAA 1 cut(s) 1145
Psp124BI GAGCTC 1 cut(s) 2100
PspCI CACGTG 1 cut(s) 1036
PspFI CCCAGC 1 cut(s) 773
PspN4I GGNNCC 4 cut(s) 645, 1277, 1991, 2776
PspPI GGNCC 2 cut(s) 1833, 2774
PsuI RGATCY 2 cut(s) 643, 1287
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 7 cut(s) 657, 849, 1664, 1739, 1771, 2447, 2618
RsaNI GTAC 7 cut(s) 656, 848, 1663, 1738, 1770, 2446, 2617
RseI CAYNNNNRTG 5 cut(s) 251, 999, 1337, 1790, 2694
Rsr2I CGGWCCG 1 cut(s) 1833
RsrII CGGWCCG 1 cut(s) 1833
SacI GAGCTC 1 cut(s) 2100
Sau3AI GATC 7 cut(s) 643, 909, 931, 1030, 1119, 1287, 2185
Sau96I GGNCC 2 cut(s) 1833, 2774
ScaI AGTACT 2 cut(s) 849, 2618
SduI GDGCHC 2 cut(s) 1723, 2100
Sfr274I CTCGAG 1 cut(s) 1180
SfuI TTCGAA 1 cut(s) 1807
SinI GGWCC 2 cut(s) 1833, 2774
SlaI CTCGAG 1 cut(s) 1180
SmiMI CAYNNNNRTG 5 cut(s) 251, 999, 1337, 1790, 2694
SmlI CTYRAG 4 cut(s) 1180, 1658, 2303, 2342
SmoI CTYRAG 4 cut(s) 1180, 1658, 2303, 2342
SphI GCATGC 1 cut(s) 2598
SsiI CCGC 8 cut(s) 317, 375, 527, 648, 1407, 1433, 1831, 1993
SspI AATATT 1 cut(s) 1149
SspMI CTAG 9 cut(s) 152, 711, 1752, 1877, 1973, 2199, 2294, 2312, 2741
SstI GAGCTC 1 cut(s) 2100
StyI CCWWGG 3 cut(s) 900, 946, 1972
TaaI ACNGT 6 cut(s) 386, 416, 481, 1045, 1369, 1769
TaiI ACGT 7 cut(s) 314, 462, 986, 1038, 2137, 2397, 2667
TaqI TCGA 6 cut(s) 343, 498, 510, 1181, 1807, 2438
TaqII GACCGA 1 cut(s) 361
TatI WGTACW 3 cut(s) 847, 2445, 2616
TauI GCSGC 1 cut(s) 1436
TfiI GAWTC 4 cut(s) 768, 1316, 2317, 2787
TscAI CASTG 3 cut(s) 743, 946, 2404
TseFI GTSAC 7 cut(s) 217, 253, 937, 1199, 1443, 2135, 2395
Tsp45I GTSAC 7 cut(s) 217, 253, 937, 1199, 1443, 2135, 2395
TspGWI ACGGA 2 cut(s) 815, 2278
TspRI CASTG 3 cut(s) 743, 946, 2404
Van91I CCANNNNNTGG 1 cut(s) 1270
Vha464I CTTAAG 2 cut(s) 2303, 2342
VpaK11BI GGWCC 2 cut(s) 1833, 2774
XapI RAATTY 6 cut(s) 868, 1077, 1191, 2103, 2348, 2471
XbaI TCTAGA 1 cut(s) 1751
XceI RCATGY 3 cut(s) 998, 2598, 2677
XhoI CTCGAG 1 cut(s) 1180
XmaJI CCTAGG 1 cut(s) 1972
XmiI GTMKAC 1 cut(s) 2131
XmnI GAANNNNTTC 2 cut(s) 1328, 2151
XspI CTAG 9 cut(s) 152, 711, 1752, 1877, 1973, 2199, 2294, 2312, 2741
ZraI GACGTC 1 cut(s) 2395
ZrmI AGTACT 2 cut(s) 849, 2618
Zsp2I ATGCAT 4 cut(s) 602, 1102, 1787, 2654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.