Rmu_sc0002187.1_g000043

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002187.1
OG0000085
Physical Location & Seq
Reverse (-)
176837 .. 180712
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002187.1_g000043.1.cds

Sequence Viewer

Length: 3471 bp
atggcgattattgcggtaattgtgacatggattggtgtcatagctactgttaagtggatatggactctagtgaagcatcggttgggttacatggttgaaaagcatgagccaacatcgcaggataatcaagagttcgtcaacatcgatgcctccagacctcttccttcaccatcagctgaatcatcagcagctcgtcaatggaattatgaggtgttcttgagtttcaggggtcctgacactcgcaagggtattacatttgaattatatgatcgactgcacaggaggggaattaaaacattcatggatgaccaagatcttcaagtaggggatactatttctcccactctactaaaagcaattgaagaatcaaggttggcaattgttgttctgtccgaaaagtatgcttcttctacttggtgtttggaggaacttacaaagatctgtgagtgcatgaaagacgacaacagaattcttccacttttttataatgtcgagcctactgatgtaagatatcagaagagaagtttcgaagaagctttcaccaaatatgaaaagtctgggcgacatagatcggagaaggtccaacaatggagagatgctttaaacaaagtggctcatttctctggttgggattcaaagaaatacaagactgaaagagaactcatcgaagacattgtggaatatgtctgtagtaaagtacaacctattgcaatcaacatgggagattttgaagaatttgaagcaacaagacaagccatgaatatggttatgaaagggctagaagatgacgaggtcactgccattggagtctacggaatgggaggcgtcggcaagacaaccatggtaaaacatatcagtgatcaagctcgaaaaaatggaaactttcgtcatgtaattatggctactatatcacaaagccctgacttgaggaaaattcaaggccaattggcagatctgttgggcttcgaattcatggaggagactgaaattggaagagcagctagattgagtaaggagataatgagaagaaataagatcctcataatccttgatgacatttgggagaggatggatatatcaagaataggaatacctagctatgaggaacttcaaaagtgtaattccaaagtcctactcaccacaaggatatggaatgtctgtcatgtcatgagatgccaagaaaagatcaccctcaatatcctatcaaaagaggattcttggacattgtttgtgagaaatgcaggaaggtcttttgaatccaccaattttgagaatgtagcgaggaaggtagctggagaatgtaggggtctaccgattgcattgatagcagttgcaagggcactcggcgataaagaattggtggaatgggaaagagcagctcaacgactagagaagtcacaaactgccaaccctgacgataaaggagatgcatctgaatgtataagattaagttatgattacttgaaagatgaggattacaagtcatgcttcttgctctgttgcttatttccagaagactatgacatccaaatagaaaacttattcaactatgtgatagggaaaggactgtttagagatgctgagaccatcgaagaagccagaggaacagcaaatttggtggttgcgcacctgaaaaattgttgcttgcttttggatagtgatgaagatggatgtgtaagaatgcatgatgtcatccgggatacagccatgataattgcaaaatctaaagacgggcatgggtttttggtaaaagctgggtctggtttaaagcattggtcatgtcgattacatgaagactactctgcaatttcactaatgaagaacaaaattcgcaagctacctgaagagttggtatgtccaaaactccagattttattactacaagagaatgctgatttaacagagattccagaaaaatttctccaaagtgcaaatgaactaagggtcttagatcttggcctcactagtgttttatcactaccccaatcattgagtctcctatcaaacctccaagctttgtatttagatttttgcaagaaaagagttgacatttccataattggaaaacttactaagcttgagattcttagtatgagaggttatcctgttaaggaattgtcaagagaattgtggagagaagtaggaaaattgactaatctaaggatgttggatgtcggtggtgttagtgtggtgggtggaggtactgtcacaattccctctaaagtgatatcaaagttgcatagattagaagaactgtacatgctgtactgtggatttgaggactggggaagtcgggttgagggagaaggagaagaaacatatattatctttgatgagttaactggtttatcaaatttgaaaattgtgcagctttgcatatctgatgcaaaatgcatcccaaaagaagttgaggtcgaaccaaattgggtttactttgatatacgtatcgatggctggtcaagcaatccatacaagcgaaatgttcctaactcaagatcctcgtctcttaacacaaccatcagtagcttaccagactggtttgtcaatgtggtgacaaggaaatcagagaagctactgtatgaagaagactgcaaaggtttaagtaacattcttatggaatatgaccatgggaggttacatggactgaagcatctctctgtaattggtcccaataagagcttgaaagagttgatgaacacaacaacacggattaaacaagaacctgtgtttgagaacttggaagagttgcatctgagtcgcctggactgcatgaaggagttgtgtgttggtgacttaccacctggttctctattcaatgtgaagttattgaggatccgcgattgtcttaacttggggaatgtactgttgccttcaaaattgttgcagagactgccaaatctggaaatagtatttctttacagaatacgtaaattggaatatgtgtttggatgtgaagggtttgagccagaacattcaaaattgagagaggtgacattgttgtggctagatgcagtaagaagcatatgtaatggtccggttccccgtgctatgttccggactcttagaagtttgttaattttccgttgtaagcctttgggaagtctcttcacatgtgatgcagcgcaatgtctttttcaattggaagagctttgggtagagaattgccctttgttggaaagagtaattgaaacaagcaaggaaacagttaacaacaacaagaggattgttctttcaaaattaaataagttagttttgaaggaacttcctgtgttgtacagtggaagcgctactactgacattgatatcgagtgtccttcattggaacacctgtatgtgaaggactgcccccatttgtcatttccaacctctgcttctgactatttccacagcaggaacgagatccaactcaatgatcaacaagagcatgacattctatatcgcagaatttggagtccaacattggaaagctga

Protein Analysis

1156

Amino Acids

132.4

Weight (kDa)

5.94

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AasI GACNNNNNNGTC 1 cut(s) 2540
Acc16I TGCGCA 1 cut(s) 1626
AccI GTMKAC 2 cut(s) 810, 1309
AccII CGCG 1 cut(s) 2838
AccIII TCCGGA 1 cut(s) 3054
AciI CCGC 2 cut(s) 14, 2836
AclWI GGATC 5 cut(s) 1030, 2490, 2827, 2840, 3394
AcsI RAATTY 9 cut(s) 468, 734, 933, 968, 1612, 1827, 1916, 2353, 3444
AcuI CTGAAG 2 cut(s) 1863, 2666
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 6 cut(s) 699, 2203, 2258, 2267, 2862, 3275
AfeI AGCGCT 1 cut(s) 3286
AfiI CCNNNNNNNGG 1 cut(s) 3172
AflIII ACRYGT 1 cut(s) 3110
AhlI ACTAGT 1 cut(s) 1964
AjnI CCWGG 2 cut(s) 2760, 2800
AjuI GAANNNNNNNTTGG 6 cut(s) 65, 97, 2928, 2960, 3355, 3387
Alw26I GTCTC 6 cut(s) 974, 1577, 2000, 2508, 2881, 3107
AlwI GGATC 5 cut(s) 1030, 2490, 2827, 2840, 3394
AlwNI CAGNNNCTG 1 cut(s) 2890
Aor13HI TCCGGA 1 cut(s) 3054
Aor51HI AGCGCT 1 cut(s) 3286
AoxI GGCC 2 cut(s) 940, 1957
ApeKI GCWGC 5 cut(s) 188, 998, 1376, 2368, 3119
ApoI RAATTY 9 cut(s) 468, 734, 933, 968, 1612, 1827, 1916, 2353, 3444
Asp700I GAANNNNTTC 1 cut(s) 1917
AspLEI GCGC 3 cut(s) 1627, 3124, 3287
AspS9I GGNCC 4 cut(s) 230, 580, 2666, 3032
AsuC2I CCSGG 1 cut(s) 1697
AsuHPI GGTGA 7 cut(s) 159, 532, 1129, 1180, 2563, 2801, 3001
AsuII TTCGAA 2 cut(s) 528, 966
AvaII GGWCC 4 cut(s) 230, 580, 2666, 3032
BaeGI GKGCMC 1 cut(s) 1342
BamHI GGATCC 1 cut(s) 2832
BarI GAAGNNNNNNTAC 2 cut(s) 3274, 3306
BbsI GAAGAC 4 cut(s) 675, 1521, 1800, 2592
BbvI GCAGC 5 cut(s) 200, 1010, 1388, 2380, 3131
BccI CCATC 6 cut(s) 178, 1063, 1595, 1661, 2444, 2525
BcgI CGANNNNNNTGC 6 cut(s) 383, 417, 779, 813, 2738, 2772
BciT130I CCWGG 2 cut(s) 2762, 2802
BciVI GTATCC 2 cut(s) 322, 1693
BclI TGATCA 2 cut(s) 859, 3412
BcnI CCSGG 1 cut(s) 1697
BcoDI GTCTC 6 cut(s) 974, 1577, 2000, 2508, 2881, 3107
BcuI ACTAGT 1 cut(s) 1964
BfaI CTAG 7 cut(s) 68, 779, 1002, 1095, 1388, 1965, 3005
BfmI CTRYAG 1 cut(s) 688
BfoI RGCGCY 1 cut(s) 3288
BfuI GTATCC 2 cut(s) 322, 1693
BglII AGATCT 4 cut(s) 313, 438, 952, 1951
BisI GCNGC 5 cut(s) 189, 999, 1377, 2369, 3120
BlsI GCNGC 5 cut(s) 190, 1000, 1378, 2370, 3121
Bme1390I CCNGG 3 cut(s) 1697, 2762, 2802
Bme18I GGWCC 4 cut(s) 230, 580, 2666, 3032
BmgT120I GGNCC 4 cut(s) 230, 580, 2666, 3032
BmiI GGNNCC 4 cut(s) 231, 2668, 2834, 3039
BmrFI CCNGG 3 cut(s) 1697, 2762, 2802
BmrI ACTGGG 1 cut(s) 2293
BmuI ACTGGG 1 cut(s) 2293
BpiI GAAGAC 4 cut(s) 675, 1521, 1800, 2592
BpmI CTGGAG 3 cut(s) 136, 1314, 1850
Bpu14I TTCGAA 2 cut(s) 528, 966
BpuEI CTTGAG 4 cut(s) 238, 946, 2099, 2476
BpuMI CCSGG 1 cut(s) 1697
Bsa29I ATCGAT 2 cut(s) 144, 2448
BsaAI YACGTR 2 cut(s) 2444, 2927
BsaHI GRCGYC 1 cut(s) 825
BsaI GGTCTC 1 cut(s) 1577
BsaJI CCNNGG 2 cut(s) 840, 2626
BsaWI WCCGGW 2 cut(s) 3034, 3054
BsaXI ACNNNNNCTCC 4 cut(s) 322, 352, 2190, 2220
Bsc4I CCNNNNNNNGG 1 cut(s) 3172
Bse1I ACTGG 3 cut(s) 2288, 2347, 2540
Bse3DI GCAATG 1 cut(s) 3131
BseAI TCCGGA 1 cut(s) 3054
BseBI CCWGG 2 cut(s) 2762, 2802
BseCI ATCGAT 2 cut(s) 144, 2448
BseDI CCNNGG 2 cut(s) 840, 2626
BseGI GGATG 9 cut(s) 310, 1074, 1524, 1676, 1692, 2169, 2176, 2394, 2954
BseLI CCNNNNNNNGG 1 cut(s) 3172
BseMI GCAATG 1 cut(s) 3131
BseMII CTCAG 2 cut(s) 1572, 2744
BseNI ACTGG 3 cut(s) 2288, 2347, 2540
BseRI GAGGAG 1 cut(s) 992
BseSI GKGCMC 1 cut(s) 1342
BseXI GCAGC 5 cut(s) 200, 1010, 1388, 2380, 3131
BseYI CCCAGC 1 cut(s) 1754
BsgI GTGCAG 2 cut(s) 260, 2387
Bsh1236I CGCG 1 cut(s) 2838
BshFI GGCC 2 cut(s) 942, 1959
BshVI ATCGAT 2 cut(s) 144, 2448
BsiSI CCGG 3 cut(s) 1696, 3035, 3055
BslFI GGGAC 1 cut(s) 2652
BslI CCNNNNNNNGG 1 cut(s) 3172
BsmAI GTCTC 6 cut(s) 974, 1577, 2000, 2508, 2881, 3107
BsmBI CGTCTC 1 cut(s) 2508
BsmFI GGGAC 1 cut(s) 2652
BsmI GAATGC 2 cut(s) 1686, 1894
BsnI GGCC 2 cut(s) 942, 1959
Bso31I GGTCTC 1 cut(s) 1577
Bsp119I TTCGAA 2 cut(s) 528, 966
Bsp1286I GDGCHC 1 cut(s) 1342
Bsp13I TCCGGA 1 cut(s) 3054
Bsp1407I TGTACA 2 cut(s) 2256, 3273
Bsp19I CCATGG 2 cut(s) 840, 2626
BspACI CCGC 2 cut(s) 14, 2836
BspANI GGCC 2 cut(s) 942, 1959
BspCNI CTCAG 2 cut(s) 1573, 2745
BspDI ATCGAT 2 cut(s) 144, 2448
BspEI TCCGGA 1 cut(s) 3054
BspFNI CGCG 1 cut(s) 2838
BspHI TCATGA 1 cut(s) 1167
BspLI GGNNCC 4 cut(s) 231, 2668, 2834, 3039
BspPI GGATC 5 cut(s) 1030, 2490, 2827, 2840, 3394
BspQI GCTCTTC 2 cut(s) 988, 3138
BspT104I TTCGAA 2 cut(s) 528, 966
BspTNI GGTCTC 1 cut(s) 1577
BsrDI GCAATG 1 cut(s) 3131
BsrGI TGTACA 2 cut(s) 2256, 3273
BsrI ACTGG 3 cut(s) 2288, 2347, 2540
BssECI CCNNGG 2 cut(s) 840, 2626
BssNI GRCGYC 1 cut(s) 825
BssT1I CCWWGG 2 cut(s) 840, 2626
Bst2UI CCWGG 2 cut(s) 2762, 2802
Bst4CI ACNGT 9 cut(s) 49, 1569, 2206, 2256, 2270, 2577, 2865, 3205, 3278
Bst6I CTCTTC 7 cut(s) 165, 512, 988, 1839, 2736, 3110, 3138
BstACI GRCGYC 1 cut(s) 825
BstAPI GCANNNNNTGC 1 cut(s) 2890
BstAUI TGTACA 2 cut(s) 2256, 3273
BstBAI YACGTR 2 cut(s) 2444, 2927
BstBI TTCGAA 2 cut(s) 528, 966
BstC8I GCNNGC 2 cut(s) 1646, 1835
BstDEI CTNAG 8 cut(s) 1581, 1940, 1948, 2073, 2087, 2159, 2753, 3062
BstDSI CCRYGG 2 cut(s) 840, 2626
BstF5I GGATG 9 cut(s) 310, 1074, 1524, 1676, 1692, 2169, 2176, 2394, 2954
BstFNI CGCG 1 cut(s) 2838
BstH2I RGCGCY 1 cut(s) 3288
BstHHI GCGC 3 cut(s) 1627, 3124, 3287
BstMAI GTCTC 6 cut(s) 974, 1577, 2000, 2508, 2881, 3107
BstMWI GCNNNNNNNGC 6 cut(s) 11, 115, 1325, 2460, 2766, 2890
BstNI CCWGG 2 cut(s) 2762, 2802
BstNSI RCATGY 2 cut(s) 2263, 3114
BstSCI CCNGG 3 cut(s) 1695, 2760, 2800
BstSFI CTRYAG 1 cut(s) 688
BstSLI GKGCMC 1 cut(s) 1342
BstSNI TACGTA 2 cut(s) 2444, 2927
BstUI CGCG 1 cut(s) 2838
BstV1I GCAGC 5 cut(s) 200, 1010, 1388, 2380, 3131
BstV2I GAAGAC 4 cut(s) 675, 1521, 1800, 2592
BstX2I RGATCY 8 cut(s) 313, 438, 952, 1035, 1951, 2495, 2832, 3399
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 8 cut(s) 313, 438, 952, 1035, 1951, 2495, 2832, 3399
Bsu15I ATCGAT 2 cut(s) 144, 2448
BsuI GTATCC 2 cut(s) 322, 1693
BsuRI GGCC 2 cut(s) 942, 1959
BsuTUI ATCGAT 2 cut(s) 144, 2448
BtgI CCRYGG 2 cut(s) 840, 2626
BtgZI GCGATG 1 cut(s) 99
BtsCI GGATG 9 cut(s) 310, 1074, 1524, 1676, 1692, 2169, 2176, 2394, 2954
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 3 cut(s) 795, 862, 3283
Cac8I GCNNGC 2 cut(s) 1646, 1835
CaiI CAGNNNCTG 1 cut(s) 2890
CciI TCATGA 1 cut(s) 1167
CfoI GCGC 3 cut(s) 1627, 3124, 3287
Cfr13I GGNCC 4 cut(s) 230, 580, 2666, 3032
ClaI ATCGAT 2 cut(s) 144, 2448
CseI GACGC 1 cut(s) 814
CsiI ACCWGGT 1 cut(s) 2800
Csp6I GTAC 6 cut(s) 698, 2202, 2257, 2266, 2861, 3274
CspCI CAANNNNNGTGG 4 cut(s) 1249, 1284, 1599, 1634
CviQI GTAC 6 cut(s) 698, 2202, 2257, 2266, 2861, 3274
DdeI CTNAG 8 cut(s) 1581, 1940, 1948, 2073, 2087, 2159, 2753, 3062
DraI TTTAAA 2 cut(s) 603, 1767
DrdI GACNNNNNNGTC 1 cut(s) 2540
DseDI GACNNNNNNGTC 1 cut(s) 2540
Eam1104I CTCTTC 7 cut(s) 165, 512, 988, 1839, 2736, 3110, 3138
EarI CTCTTC 7 cut(s) 165, 512, 988, 1839, 2736, 3110, 3138
Eco105I TACGTA 2 cut(s) 2444, 2927
Eco130I CCWWGG 2 cut(s) 840, 2626
Eco31I GGTCTC 1 cut(s) 1577
Eco32I GATATC 3 cut(s) 512, 2229, 3304
Eco47I GGWCC 4 cut(s) 230, 580, 2666, 3032
Eco47III AGCGCT 1 cut(s) 3286
Eco57I CTGAAG 2 cut(s) 1863, 2666
EcoO109I RGGNCCY 1 cut(s) 230
EcoRI GAATTC 2 cut(s) 468, 968
EcoRII CCWGG 2 cut(s) 2760, 2800
EcoRV GATATC 3 cut(s) 512, 2229, 3304
EcoT14I CCWWGG 2 cut(s) 840, 2626
EcoT22I ATGCAT 3 cut(s) 1432, 1686, 2396
ErhI CCWWGG 2 cut(s) 840, 2626
Esp3I CGTCTC 1 cut(s) 2508
FalI AAGNNNNNCTT 2 cut(s) 1472, 1504
FaqI GGGAC 1 cut(s) 2652
FauNDI CATATG 1 cut(s) 3023
FbaI TGATCA 2 cut(s) 859, 3412
FblI GTMKAC 2 cut(s) 810, 1309
Fnu4HI GCNGC 5 cut(s) 189, 999, 1377, 2369, 3120
FokI GGATG 9 cut(s) 317, 1081, 1511, 1679, 1683, 2176, 2183, 2381, 2961
Fsp4HI GCNGC 5 cut(s) 189, 999, 1377, 2369, 3120
FspBI CTAG 7 cut(s) 68, 779, 1002, 1095, 1388, 1965, 3005
FspI TGCGCA 1 cut(s) 1626
GlaI GCGC 3 cut(s) 1626, 3123, 3286
GluI GCNGC 5 cut(s) 189, 999, 1377, 2369, 3120
GsaI CCCAGC 1 cut(s) 1758
GsuI CTGGAG 3 cut(s) 136, 1314, 1850
HaeII RGCGCY 1 cut(s) 3288
HaeIII GGCC 2 cut(s) 942, 1959
HapII CCGG 3 cut(s) 1696, 3035, 3055
HgaI GACGC 1 cut(s) 814
HhaI GCGC 3 cut(s) 1627, 3124, 3287
Hin1I GRCGYC 1 cut(s) 825
Hin6I GCGC 3 cut(s) 1625, 3122, 3285
HinP1I GCGC 3 cut(s) 1625, 3122, 3285
HincII GTYRAC 4 cut(s) 139, 2047, 2340, 3208
HindII GTYRAC 4 cut(s) 139, 2047, 2340, 3208
HindIII AAGCTT 3 cut(s) 534, 2013, 2075
HpaI GTTAAC 2 cut(s) 2340, 3208
HpaII CCGG 3 cut(s) 1696, 3035, 3055
HphI GGTGA 7 cut(s) 159, 532, 1129, 1180, 2563, 2801, 3001
Hpy166II GTNNAC 7 cut(s) 139, 811, 1310, 2047, 2340, 2431, 3208
Hpy188I TCNGA 8 cut(s) 394, 516, 574, 1435, 2383, 2566, 2754, 3376
Hpy8I GTNNAC 7 cut(s) 139, 811, 1310, 2047, 2340, 2431, 3208
Hpy99I CGWCG 1 cut(s) 830
HpyCH4III ACNGT 9 cut(s) 49, 1569, 2206, 2256, 2270, 2577, 2865, 3205, 3278
HpyCH4IV ACGT 2 cut(s) 2443, 2926
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 115, 1325, 2460, 2766, 2890
HpyF3I CTNAG 8 cut(s) 1581, 1940, 1948, 2073, 2087, 2159, 2753, 3062
HpySE526I ACGT 2 cut(s) 2443, 2926
Hsp92I GRCGYC 1 cut(s) 825
HspAI GCGC 3 cut(s) 1625, 3122, 3285
Kpn2I TCCGGA 1 cut(s) 3054
Ksp22I TGATCA 2 cut(s) 859, 3412
KspAI GTTAAC 2 cut(s) 2340, 3208
LguI GCTCTTC 2 cut(s) 988, 3138
Lsp1109I GCAGC 5 cut(s) 200, 1010, 1388, 2380, 3131
MabI ACCWGGT 1 cut(s) 2800
MaeI CTAG 7 cut(s) 68, 779, 1002, 1095, 1388, 1965, 3005
MaeII ACGT 2 cut(s) 2443, 2926
MfeI CAATTG 4 cut(s) 357, 378, 944, 3137
MflI RGATCY 8 cut(s) 313, 438, 952, 1035, 1951, 2495, 2832, 3399
MhlI GDGCHC 1 cut(s) 1342
MlyI GAGTC 6 cut(s) 58, 816, 2002, 2764, 3052, 3460
MmeI TCCRAC 5 cut(s) 607, 2148, 3153, 3386, 3427
Mph1103I ATGCAT 3 cut(s) 1432, 1686, 2396
MroI TCCGGA 1 cut(s) 3054
MroXI GAANNNNTTC 1 cut(s) 1917
MslI CAYNNNNRTG 6 cut(s) 761, 855, 1435, 2612, 2998, 3330
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 3 cut(s) 1696, 3035, 3055
MspR9I CCNGG 3 cut(s) 1697, 2762, 2802
MunI CAATTG 4 cut(s) 357, 378, 944, 3137
Mva1269I GAATGC 2 cut(s) 1686, 1894
MvaI CCWGG 2 cut(s) 2762, 2802
MvnI CGCG 1 cut(s) 2838
MwoI GCNNNNNNNGC 6 cut(s) 11, 115, 1325, 2460, 2766, 2890
NciI CCSGG 1 cut(s) 1697
NcoI CCATGG 2 cut(s) 840, 2626
NdeI CATATG 1 cut(s) 3023
NlaIV GGNNCC 4 cut(s) 231, 2668, 2834, 3039
NmeAIII GCCGAG 1 cut(s) 1323
NmuCI GTSAC 7 cut(s) 22, 793, 1395, 2206, 2551, 2789, 2989
NsbI TGCGCA 1 cut(s) 1626
NsiI ATGCAT 3 cut(s) 1432, 1686, 2396
NspI RCATGY 2 cut(s) 2263, 3114
NspV TTCGAA 2 cut(s) 528, 966
PagI TCATGA 1 cut(s) 1167
PciI ACATGT 1 cut(s) 3110
PciSI GCTCTTC 2 cut(s) 988, 3138
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PctI GAATGC 2 cut(s) 1686, 1894
PdmI GAANNNNTTC 1 cut(s) 1917
PfeI GAWTC 7 cut(s) 179, 365, 632, 1214, 1256, 1906, 2083
PflFI GACNNNGTC 1 cut(s) 791
PfoI TCCNGGA 1 cut(s) 1695
PkrI GCNGC 5 cut(s) 190, 1000, 1378, 2370, 3121
PleI GAGTC 6 cut(s) 58, 815, 2001, 2763, 3052, 3459
PpsI GAGTC 6 cut(s) 58, 815, 2001, 2763, 3052, 3459
Ppu21I YACGTR 2 cut(s) 2444, 2927
PpuMI RGGWCCY 1 cut(s) 230
PscI ACATGT 1 cut(s) 3110
PsiI TTATAA 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 2760, 2800
PspFI CCCAGC 1 cut(s) 1754
PspGI CCWGG 2 cut(s) 2760, 2800
PspN4I GGNNCC 4 cut(s) 231, 2668, 2834, 3039
PspPI GGNCC 4 cut(s) 230, 580, 2666, 3032
PspPPI RGGWCCY 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 2890
PsuI RGATCY 8 cut(s) 313, 438, 952, 1035, 1951, 2495, 2832, 3399
PsyI GACNNNGTC 1 cut(s) 791
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 6 cut(s) 699, 2203, 2258, 2267, 2862, 3275
RsaNI GTAC 6 cut(s) 698, 2202, 2257, 2266, 2861, 3274
RseI CAYNNNNRTG 6 cut(s) 761, 855, 1435, 2612, 2998, 3330
SapI GCTCTTC 2 cut(s) 988, 3138
SatI GCNGC 5 cut(s) 189, 999, 1377, 2369, 3120
Sau96I GGNCC 4 cut(s) 230, 580, 2666, 3032
SchI GAGTC 6 cut(s) 58, 816, 2002, 2764, 3052, 3460
ScrFI CCNGG 3 cut(s) 1697, 2762, 2802
SduI GDGCHC 1 cut(s) 1342
SexAI ACCWGGT 1 cut(s) 2800
SfcI CTRYAG 1 cut(s) 688
SfuI TTCGAA 2 cut(s) 528, 966
SinI GGWCC 4 cut(s) 230, 580, 2666, 3032
SmiMI CAYNNNNRTG 6 cut(s) 761, 855, 1435, 2612, 2998, 3330
SmlI CTYRAG 4 cut(s) 217, 925, 2078, 2491
SmoI CTYRAG 4 cut(s) 217, 925, 2078, 2491
SnaBI TACGTA 2 cut(s) 2444, 2927
SpeI ACTAGT 1 cut(s) 1964
SsiI CCGC 2 cut(s) 14, 2836
SspMI CTAG 7 cut(s) 68, 779, 1002, 1095, 1388, 1965, 3005
StyD4I CCNGG 3 cut(s) 1695, 2760, 2800
StyI CCWWGG 2 cut(s) 840, 2626
TaaI ACNGT 9 cut(s) 49, 1569, 2206, 2256, 2270, 2577, 2865, 3205, 3278
TaiI ACGT 2 cut(s) 2446, 2929
TatI WGTACW 5 cut(s) 697, 2256, 2265, 2860, 3273
TfiI GAWTC 7 cut(s) 179, 365, 632, 1214, 1256, 1906, 2083
TscAI CASTG 3 cut(s) 802, 862, 3283
TseFI GTSAC 7 cut(s) 22, 793, 1395, 2206, 2551, 2789, 2989
TseI GCWGC 5 cut(s) 188, 998, 1376, 2368, 3119
Tsp45I GTSAC 7 cut(s) 22, 793, 1395, 2206, 2551, 2789, 2989
TspGWI ACGGA 3 cut(s) 828, 2722, 3071
TspRI CASTG 3 cut(s) 802, 862, 3283
Tth111I GACNNNGTC 1 cut(s) 791
VpaK11BI GGWCC 4 cut(s) 230, 580, 2666, 3032
XapI RAATTY 9 cut(s) 468, 734, 933, 968, 1612, 1827, 1916, 2353, 3444
XceI RCATGY 2 cut(s) 2263, 3114
XmiI GTMKAC 2 cut(s) 810, 1309
XmnI GAANNNNTTC 1 cut(s) 1917
XspI CTAG 7 cut(s) 68, 779, 1002, 1095, 1388, 1965, 3005
Zsp2I ATGCAT 3 cut(s) 1432, 1686, 2396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.