Rw5G039570

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000085
Physical Location & Seq
Forward (+)
69815207 .. 69819563
4357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G039570.1

Sequence Viewer

Length: 1872 bp
ATGAAGGAGCTAACTGGGAGTCTAATTGGTTTAGAATCTAGATGGTCTGAAAGAGAACTTGTGGAAGACATTGTGAAATTTGTGTGGGATAAAGTACTTCAAGCACCTCTCGGAATTGAGTCTGAAATGTCTAACAGAGACTTTGAAGCATTTCAAGCAACAAGCCAAGCCATGGAGAAGGTCATGATGGCACTAAAAGATGATAAGGCTGCTGTCATCGGGGTGTATGGAAGGGGAGGCGTCGGAAAGACAACTCTAGTGAAACATGTTGGTTCACAAGCCAAAAAAGATAAACTTTTTGATCAAGTGATTGTGGTTGTCATATCTCAAAGCCCCGACTTGAGAAAAATTCAAGGCACATTGGCAGATTTGCTTGGCTTGAATTTAGAGAATAAATCAGAATTTGAATTTGGATCTGAAAGAGCAGTTAAAAGAGCCATTAGATTGAAGGAGGGGATAATGAGAGGAAACAGGATCCTCATAATCTTGGATGACCTTTGGAAAAGAATAGAATTGTCAGCCATAGGAATTCCAAGCTGTTATGAGCTTCAAAAATGCAATTCGAAGGTCCTACTTACTACAAGGAGATTAAATGTTTGTCATTCCATGGAGAGCCAAACAAACATTCCTCTCAACATCTTATCAGAAGAAGATTCTTTAAAATTGTTTGTGAAGATAGCAAGAAAGTCTTCTGACATATCAAACAGTTTCTACGATGTAGCAAGGAAGGTAGCTAGAGAATGTGCTGGTCTTCCAGTTGCGTTGATAGCAGTTGCAAGAGCACTTGCAGATAAGGACTTGGACGACTGGAAAGAAGCAGCTTGGCGACTAAAAGCGGCTCATCCTGGCAACCCTGAAGATAAGAGAGATGTGTTCAGATGTATAAGGTTAAGCTATGATTACTTGGAAACTGATGATGCCAAGTCATTCTTCTTGCTTTGCTGTCTGTTCCCAGAAGATAGTGATATTGCAATCGAAGACTTGCTGATGTATGCGATCGGGAAAGGATTATTTCAGGATGCCGACACAATCAAAGAAGCCAGAGCCAGAGCACATTCGGTGGCAAAGTACCTTCAAGCCTCTGGTTTGCTTTTGGAGAGTGAATGCTACGGATGTGTAAGGATACATAATGTCATTCGTGATGGTGCCATATCAATTTCATTATCGGAAGATGGCCCTCAGTTTGTGGTAAAAGCAGGTTGTGAATTAAAGGATTGGCCAACAATTAGTGCACATGAAGGCTACTCTGGAATCTCACTAATGAAGAATGAAATTCAGGAGCTTCCCAAAATGTTGGTATGTCCAAAACTACAGATTTTACTATTACAAAGTAATGTTCATATAAGAAAGATCCCACAAACATTTTTTCAAACTCCTAATGAACTGAGGGTCTTAGATCTTAGAGAGACTAGTATTTCGTTACTTCCTACATCATTCAGGCTCCTAACCAACCTCCAAGCTTTATATATAGATGATTGCAAGAGAATAACTAACGTCTCCATACTCAGAAAACTGAAGAAACTTGAGATTCTTAGTATGAGAAAAATTTGTATCAAGGAATTGCCGAAAGAAATAGGAGAATTAACCACTCTAAAGATGTTGGATGTCACTAATGCATACTTTAAAATTGTTCCATCTCAAGTGATATCAAAGTTGCATGGATTAGAAGAACTGTACATGGACGGTGCATTTTGGAACTGGGGTAAGGGAGGCTTATTTTCTTTTGCTCTTGGCAAGAATTCTGGCTTTGATGAGTTAACAGGCTTATCACGTTTAAACAGTTTGAAGATTGTGGCAACCACGCCTACCTCAAGAAGAGAGATTCATCTACTGATCATGGAGAAAGGCAAGGATTCTTCAATTGGCTTTTAA

Protein Analysis

623

Amino Acids

69.97

Weight (kDa)

8.26

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 56 - 229 9.5e-31 NB-ARC domain
LRR_8 PF13855 435 - 493 4.9e-09 Leucine rich repeat
LRR_14 PF23598 438 - 514 5.2e-06 Leucine-rich repeat region
LRR_14 PF23598 499 - 597 8.4e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000085)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05721 FvH4_2g05721 FvH4_3g35820 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35890 FvH4_3g35920 FvH4_3g38670 FvH4_3g43650 FvH4_3g43650 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44000 FvH4_3g44070 FvH4_5g01271 FvH4_5g02970 FvH4_5g02970 FvH4_5g02970 FvH4_5g27640 FvH4_5g27640 FvH4_5g27640 FvH4_6g05393 FvH4_6g52372 FvH4_7g06680 FvH4_7g12671 FvH4_7g13640 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31110 FvH4_7g31510
malus_domestica MD09G1227900.v1.1 MD11G1019800.v1.1 MD11G1020500.v1.1
prunus_persica Prupe.1G542800_v2.0.a1 Prupe.1G542800_v2.0.a1
pyrus_communis pycom11g01460 pycom11g01490 pycom11g01530 pycom11g01570 pycom15g35490
rosa_chinensis RchiOBHm_Chr1g0353711 RchiOBHm_Chr3g0473211 RchiOBHm_Chr3g0473291 RchiOBHm_Chr3g0473341 RchiOBHm_Chr3g0473381 RchiOBHm_Chr3g0473391 RchiOBHm_Chr3g0473441 RchiOBHm_Chr3g0473481 RchiOBHm_Chr3g0473531 RchiOBHm_Chr3g0473561 RchiOBHm_Chr3g0473571 RchiOBHm_Chr3g0483491 RchiOBHm_Chr3g0488681 RchiOBHm_Chr5g0059311 RchiOBHm_Chr5g0061291 RchiOBHm_Chr5g0061301 RchiOBHm_Chr5g0061331 RchiOBHm_Chr5g0064081 RchiOBHm_Chr5g0064111 RchiOBHm_Chr5g0064151 RchiOBHm_Chr5g0064171 RchiOBHm_Chr5g0069721 RchiOBHm_Chr5g0069731 RchiOBHm_Chr5g0069741 RchiOBHm_Chr5g0078251 RchiOBHm_Chr5g0078301 RchiOBHm_Chr5g0079511 RchiOBHm_Chr5g0079791 RchiOBHm_Chr5g0079811 RchiOBHm_Chr5g0079861 RchiOBHm_Chr5g0079881 RchiOBHm_Chr5g0080241 RchiOBHm_Chr5g0080281 RchiOBHm_Chr5g0080371 RchiOBHm_Chr5g0080671 RchiOBHm_Chr6g0256601 RchiOBHm_Chr6g0274791 RchiOBHm_Chr7g0221971 RchiOBHm_Chr7g0221981
rosa_laevigata RLG00000002106 RLG00000013523 RLG00000014806 RLG00000024010 RLG00000035451 RLG00000035455 RLG00000035464 RLG00000035705 RLG00000036094 RLG00000036817 RLG00000036825 RLG00000036826 RLG00000036848
rosa_multiflora Rmu_co8200400.1_g000001 Rmu_co8473147.1_g000001 Rmu_sc0000394.1_g000030 Rmu_sc0000555.1_g000030 Rmu_sc0000595.1_g000002 Rmu_sc0000595.1_g000006 Rmu_sc0000595.1_g000029 Rmu_sc0000689.1_g000018 Rmu_sc0000689.1_g000032 Rmu_sc0000689.1_g000041 Rmu_sc0000738.1_g000025 Rmu_sc0000826.1_g000016 Rmu_sc0000908.1_g000016 Rmu_sc0000908.1_g000019 Rmu_sc0000908.1_g000021 Rmu_sc0001208.1_g000087 Rmu_sc0001639.1_g000004 Rmu_sc0002075.1_g000004 Rmu_sc0002075.1_g000009 Rmu_sc0002187.1_g000042 Rmu_sc0002187.1_g000043 Rmu_sc0002187.1_g000060 Rmu_sc0002513.1_g000025 Rmu_sc0002983.1_g000008 Rmu_sc0003012.1_g000004 Rmu_sc0003069.1_g000019 Rmu_sc0003069.1_g000020 Rmu_sc0003069.1_g000047 Rmu_sc0003069.1_g000051 Rmu_sc0003069.1_g000053 Rmu_sc0003131.1_g000014 Rmu_sc0003514.1_g000003 Rmu_sc0003547.1_g000006 Rmu_sc0003547.1_g000008 Rmu_sc0004325.1_g000021 Rmu_sc0004482.1_g000004 Rmu_sc0004482.1_g000022 Rmu_sc0005006.1_g000004 Rmu_sc0005006.1_g000009 Rmu_sc0005006.1_g000010 Rmu_sc0005194.1_g000002 Rmu_sc0005194.1_g000003 Rmu_sc0005834.1_g000002 Rmu_sc0005947.1_g000023 Rmu_sc0006792.1_g000026 Rmu_sc0007612.1_g000001 Rmu_sc0007793.1_g000024 Rmu_sc0007793.1_g000025 Rmu_sc0008050.1_g000015 Rmu_sc0008336.1_g000003 Rmu_sc0010248.1_g000002 Rmu_sc0011921.1_g000001 Rmu_sc0011921.1_g000006 Rmu_sc0012138.1_g000001 Rmu_sc0016164.1_g000015 Rmu_sc0018807.1_g000005 Rmu_sc0030948.1_g000001 Rmu_sc0042587.1_g000001 Rmu_ssc0000170.1_g000020 Rmu_ssc0000170.1_g000026 Rmu_ssc0000170.1_g000029
rosa_roxburghii Rroxscaffold_1G00003040 Rroxscaffold_1G00003080 Rroxscaffold_1G00003430 Rroxscaffold_1G00003490 Rroxscaffold_1G00011370 Rroxscaffold_1G00016780 Rroxscaffold_1G00019390 Rroxscaffold_1G00019400 Rroxscaffold_1G00019420 Rroxscaffold_1G00021170 Rroxscaffold_3G00237530 Rroxscaffold_3G00246470 Rroxscaffold_4G00301730 Rroxscaffold_4G00309300 Rroxscaffold_6G00398250 Rroxscaffold_6G00398270 Rroxscaffold_6G00398280 Rroxscaffold_6G00408070 Rroxscaffold_7G00193940 Rroxscaffold_7G00209570
rosa_rugosa Rorug01G0237100 Rorug03G0098200 Rorug03G0132200 Rorug03G0132400 Rorug03G0132500 Rorug03G0132500 Rorug03G0132500 Rorug03G0132700 Rorug03G0132800 Rorug03G0205900 Rorug03G0206100 Rorug03G0206100 Rorug03G0206300 Rorug03G0206400 Rorug03G0206400 Rorug03G0240700 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug03G0241800 Rorug05G0322400 Rorug05G0339300 Rorug05G0339500 Rorug05G0362200 Rorug05G0362300 Rorug05G0362400 Rorug05G0362600 Rorug05G0362700 Rorug05G0362800 Rorug05G0363100 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0399200 Rorug05G0459900 Rorug05G0464600 Rorug05G0465000 Rorug05G0465100 Rorug05G0465200 Rorug05G0466000 Rorug05G0466300 Rorug05G0466400 Rorug05G0466500 Rorug05G0466600 Rorug05G0466700 Rorug05G0466700 Rorug05G0466700 Rorug05G0466800 Rorug05G0466900 Rorug05G0468100 Rorug05G0562200 Rorug05G0562300 Rorug06G0084400 Rorug06G0084500 Rorug06G0084500 Rorug06G0084600 Rorug07G0136400 Rorug07G0204500 Rorug07G0204600 Rorug07G0221500
rosa_samantha Rh5CG438200 Rh5CG569900 Rh6AG080700 Rh7CG362800 Rh7DG275900 Rh7DG343100
rosa_wichuraiana Rw0G004790 Rw0G008160 Rw0G008170 Rw0G008200 Rw0G008210 Rw0G012980 Rw0G013670 Rw0G019920 Rw0G020380 Rw0G022790 Rw0G022800 Rw0G023350 Rw1G005530 Rw1G021740 Rw3G013910 Rw3G015980 Rw3G023020 Rw3G023030 Rw3G025580 Rw5G027180 Rw5G037760 Rw5G037770 Rw5G037780 Rw5G037790 Rw5G037810 Rw5G037820 Rw5G039500 Rw5G039520 Rw5G039530 Rw5G039540 Rw5G039550 Rw5G039570 Rw5G039580 Rw5G039610 Rw5G039630 Rw5G039660 Rw5G042590 Rw5G047840 Rw5G048170 Rw5G048400 Rw5G048470 Rw5G048540 Rw6G007030 Rw6G007080 Rw6G007460 Rw6G017430 Rw7G023150 Rw7G023260 Rw7G029260 Rw7G029270 Rw7G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1187
AccB1I GGYRCC 1 cut(s) 1145
AccB7I CCANNNNNTGG 1 cut(s) 172
AciI CCGC 1 cut(s) 836
AclWI GGATC 4 cut(s) 421, 469, 482, 1345
AcoI YGGCCR 1 cut(s) 1217
AcsI RAATTY 9 cut(s) 77, 348, 382, 401, 407, 528, 1272, 1545, 1738
AcuI CTGAAG 2 cut(s) 876, 1535
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 3 cut(s) 96, 1070, 1676
AfiI CCNNNNNNNGG 1 cut(s) 172
AflIII ACRYGT 1 cut(s) 265
AhlI ACTAGT 1 cut(s) 1409
AjnI CCWGG 1 cut(s) 844
AjuI GAANNNNNNNTTGG 6 cut(s) 393, 425, 609, 641, 914, 946
AluBI AGCT 8 cut(s) 10, 537, 547, 734, 821, 894, 1282, 1460
AluI AGCT 8 cut(s) 10, 537, 547, 734, 821, 894, 1282, 1460
Alw21I GWGCWC 3 cut(s) 784, 1054, 1234
Alw26I GTCTC 3 cut(s) 132, 1400, 1501
Alw44I GTGCAC 1 cut(s) 1230
AlwI GGATC 4 cut(s) 421, 469, 482, 1345
AoxI GGCC 2 cut(s) 1174, 1217
ApaLI GTGCAC 1 cut(s) 1230
ApeKI GCWGC 2 cut(s) 209, 818
ApoI RAATTY 9 cut(s) 77, 348, 382, 401, 407, 528, 1272, 1545, 1738
ArsI GACNNNNNNTTYG 2 cut(s) 1399, 1431
Asp700I GAANNNNTTC 2 cut(s) 150, 688
AspS9I GGNCC 2 cut(s) 568, 1175
AsuII TTCGAA 1 cut(s) 563
AvaII GGWCC 1 cut(s) 568
BaeGI GKGCMC 1 cut(s) 1234
BalI TGGCCA 1 cut(s) 1219
BamHI GGATCC 1 cut(s) 474
BanI GGYRCC 1 cut(s) 1145
BbsI GAAGAC 4 cut(s) 72, 681, 743, 984
Bbv12I GWGCWC 3 cut(s) 784, 1054, 1234
BbvI GCAGC 2 cut(s) 196, 830
BccI CCATC 5 cut(s) 36, 181, 1136, 1166, 1642
BciT130I CCWGG 1 cut(s) 846
BciVI GTATCC 1 cut(s) 1116
BclI TGATCA 2 cut(s) 301, 1833
BcoDI GTCTC 3 cut(s) 132, 1400, 1501
BcuI ACTAGT 1 cut(s) 1409
BfaI CTAG 4 cut(s) 39, 257, 735, 1410
BfmI CTRYAG 1 cut(s) 1310
BfuAI ACCTGC 1 cut(s) 1187
BfuI GTATCC 1 cut(s) 1116
BglII AGATCT 1 cut(s) 1396
BisI GCNGC 3 cut(s) 210, 819, 837
BlsI GCNGC 3 cut(s) 211, 820, 838
BmcAI AGTACT 1 cut(s) 96
Bme1390I CCNGG 1 cut(s) 846
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 2 cut(s) 568, 1175
BmiI GGNNCC 3 cut(s) 476, 1147, 1442
BmrFI CCNGG 1 cut(s) 846
BmrI ACTGGG 2 cut(s) 24, 1708
BmsI GCATC 2 cut(s) 907, 1009
BmuI ACTGGG 2 cut(s) 24, 1708
BpiI GAAGAC 4 cut(s) 72, 681, 743, 984
Bpu14I TTCGAA 1 cut(s) 563
BpuEI CTTGAG 4 cut(s) 361, 1544, 1623, 1795
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 171, 606
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse1I ACTGG 4 cut(s) 19, 755, 812, 1703
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 171, 606
BseGI GGATG 5 cut(s) 496, 841, 1024, 1118, 1609
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 3 cut(s) 1193, 1376, 1519
BseNI ACTGG 4 cut(s) 19, 755, 812, 1703
BseSI GKGCMC 1 cut(s) 1234
BseXI GCAGC 2 cut(s) 196, 830
Bsh1285I CGRYCG 1 cut(s) 999
BshFI GGCC 2 cut(s) 1176, 1219
BshNI GGYRCC 1 cut(s) 1145
BsiEI CGRYCG 1 cut(s) 999
BsiHKAI GWGCWC 3 cut(s) 784, 1054, 1234
BslI CCNNNNNNNGG 1 cut(s) 172
BsmAI GTCTC 3 cut(s) 132, 1400, 1501
BsmBI CGTCTC 1 cut(s) 1501
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 2 cut(s) 1176, 1219
Bsp119I TTCGAA 1 cut(s) 563
Bsp1286I GDGCHC 3 cut(s) 784, 1054, 1234
Bsp1407I TGTACA 1 cut(s) 1674
Bsp143I GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
Bsp19I CCATGG 2 cut(s) 171, 606
BspACI CCGC 1 cut(s) 836
BspANI GGCC 2 cut(s) 1176, 1219
BspCNI CTCAG 3 cut(s) 1192, 1377, 1518
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 3 cut(s) 476, 1147, 1442
BspMI ACCTGC 1 cut(s) 1187
BspPI GGATC 4 cut(s) 421, 469, 482, 1345
BspT104I TTCGAA 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 1145
BsrGI TGTACA 1 cut(s) 1674
BsrI ACTGG 4 cut(s) 19, 755, 812, 1703
BssECI CCNNGG 2 cut(s) 171, 606
BssMI GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
BssNI GRCGYC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 171, 606
Bst2UI CCWGG 1 cut(s) 846
Bst4CI ACNGT 4 cut(s) 707, 1674, 1685, 1781
Bst6I CTCTTC 1 cut(s) 1810
BstACI GRCGYC 1 cut(s) 240
BstAUI TGTACA 1 cut(s) 1674
BstBI TTCGAA 1 cut(s) 563
BstDEI CTNAG 6 cut(s) 1179, 1385, 1393, 1400, 1505, 1532
BstDSI CCRYGG 2 cut(s) 171, 606
BstF5I GGATG 5 cut(s) 496, 841, 1024, 1118, 1609
BstKTI GATC 7 cut(s) 304, 416, 477, 999, 1353, 1399, 1836
BstMAI GTCTC 3 cut(s) 132, 1400, 1501
BstMBI GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
BstMCI CGRYCG 1 cut(s) 999
BstMWI GCNNNNNNNGC 2 cut(s) 155, 767
BstNI CCWGG 1 cut(s) 846
BstNSI RCATGY 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 844
BstSFI CTRYAG 1 cut(s) 1310
BstSLI GKGCMC 1 cut(s) 1234
BstV1I GCAGC 2 cut(s) 196, 830
BstV2I GAAGAC 4 cut(s) 72, 681, 743, 984
BstX2I RGATCY 4 cut(s) 413, 474, 1350, 1396
BstXI CCANNNNNNTGG 1 cut(s) 1294
BstYI RGATCY 4 cut(s) 413, 474, 1350, 1396
BsuI GTATCC 1 cut(s) 1116
BsuRI GGCC 2 cut(s) 1176, 1219
BtgI CCRYGG 2 cut(s) 171, 606
BtsCI GGATG 5 cut(s) 496, 841, 1024, 1118, 1609
BveI ACCTGC 1 cut(s) 1187
CciI TCATGA 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 568, 1175
CseI GACGC 1 cut(s) 229
Csp6I GTAC 3 cut(s) 95, 1069, 1675
CviAII CATG 8 cut(s) 172, 184, 266, 607, 1235, 1658, 1678, 1837
CviQI GTAC 3 cut(s) 95, 1069, 1675
DdeI CTNAG 6 cut(s) 1179, 1385, 1393, 1400, 1505, 1532
DpnI GATC 7 cut(s) 303, 415, 476, 998, 1352, 1398, 1835
DpnII GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
DraI TTTAAA 3 cut(s) 660, 1624, 1776
EaeI YGGCCR 1 cut(s) 1217
Eam1104I CTCTTC 1 cut(s) 1810
EarI CTCTTC 1 cut(s) 1810
Eco130I CCWWGG 2 cut(s) 171, 606
Eco32I GATATC 1 cut(s) 1647
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 2 cut(s) 876, 1535
EcoO109I RGGNCCY 1 cut(s) 568
EcoRI GAATTC 2 cut(s) 528, 1738
EcoRII CCWGG 1 cut(s) 844
EcoRV GATATC 1 cut(s) 1647
EcoT14I CCWWGG 2 cut(s) 171, 606
EcoT22I ATGCAT 1 cut(s) 1618
ErhI CCWWGG 2 cut(s) 171, 606
Esp3I CGTCTC 1 cut(s) 1501
FaeI CATG 8 cut(s) 175, 187, 269, 610, 1238, 1661, 1681, 1840
FalI AAGNNNNNCTT 8 cut(s) 279, 311, 673, 705, 914, 946, 1697, 1729
FatI CATG 8 cut(s) 171, 183, 265, 606, 1234, 1657, 1677, 1836
FbaI TGATCA 2 cut(s) 301, 1833
Fnu4HI GCNGC 3 cut(s) 210, 819, 837
FokI GGATG 5 cut(s) 503, 828, 1031, 1125, 1616
Fsp4HI GCNGC 3 cut(s) 210, 819, 837
FspBI CTAG 4 cut(s) 39, 257, 735, 1410
GluI GCNGC 3 cut(s) 210, 819, 837
HaeIII GGCC 2 cut(s) 1176, 1219
HgaI GACGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 8 cut(s) 175, 187, 269, 610, 1238, 1661, 1681, 1840
HincII GTYRAC 1 cut(s) 1758
HindII GTYRAC 1 cut(s) 1758
HindIII AAGCTT 1 cut(s) 1458
HinfI GANTC 8 cut(s) 19, 35, 119, 653, 1251, 1528, 1822, 1853
HpaI GTTAAC 1 cut(s) 1758
Hpy166II GTNNAC 3 cut(s) 275, 1232, 1758
Hpy188III TCNNGA 8 cut(s) 39, 184, 1000, 1016, 1139, 1248, 1277, 1812
Hpy8I GTNNAC 3 cut(s) 275, 1232, 1758
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 7 cut(s) 172, 225, 442, 559, 721, 1082, 1232
HpyCH4III ACNGT 4 cut(s) 707, 1674, 1685, 1781
HpyCH4IV ACGT 2 cut(s) 1494, 1771
HpyCH4V TGCA 9 cut(s) 558, 776, 788, 971, 1232, 1479, 1616, 1657, 1688
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 767
HpyF3I CTNAG 6 cut(s) 1179, 1385, 1393, 1400, 1505, 1532
HpySE526I ACGT 2 cut(s) 1494, 1771
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 8 cut(s) 175, 187, 269, 610, 1238, 1661, 1681, 1840
Ksp22I TGATCA 2 cut(s) 301, 1833
KspAI GTTAAC 1 cut(s) 1758
Kzo9I GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
LmnI GCTCC 3 cut(s) 7, 1279, 1446
Lsp1109I GCAGC 2 cut(s) 196, 830
LweI GCATC 2 cut(s) 907, 1009
MaeI CTAG 4 cut(s) 39, 257, 735, 1410
MaeII ACGT 2 cut(s) 1494, 1771
MaeIII GTNAC 2 cut(s) 1419, 1606
MalI GATC 7 cut(s) 303, 415, 476, 998, 1352, 1398, 1835
MboI GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
MfeI CAATTG 1 cut(s) 1860
MflI RGATCY 4 cut(s) 413, 474, 1350, 1396
MhlI GDGCHC 3 cut(s) 784, 1054, 1234
MlsI TGGCCA 1 cut(s) 1219
MluNI TGGCCA 1 cut(s) 1219
MlyI GAGTC 2 cut(s) 28, 128
MmeI TCCRAC 2 cut(s) 223, 1581
Mox20I TGGCCA 1 cut(s) 1219
Mph1103I ATGCAT 1 cut(s) 1618
MroXI GAANNNNTTC 2 cut(s) 150, 688
MscI TGGCCA 1 cut(s) 1219
MslI CAYNNNNRTG 1 cut(s) 221
Msp20I TGGCCA 1 cut(s) 1219
MspR9I CCNGG 1 cut(s) 846
MssI GTTTAAAC 1 cut(s) 1776
MunI CAATTG 1 cut(s) 1860
Mva1269I GAATGC 1 cut(s) 1109
MvaI CCWGG 1 cut(s) 846
MwoI GCNNNNNNNGC 2 cut(s) 155, 767
NcoI CCATGG 2 cut(s) 171, 606
NdeII GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
NlaIII CATG 8 cut(s) 175, 187, 269, 610, 1238, 1661, 1681, 1840
NlaIV GGNNCC 3 cut(s) 476, 1147, 1442
NmuCI GTSAC 1 cut(s) 1606
NsiI ATGCAT 1 cut(s) 1618
NspI RCATGY 1 cut(s) 269
NspV TTCGAA 1 cut(s) 563
PagI TCATGA 1 cut(s) 183
PciI ACATGT 1 cut(s) 265
PctI GAATGC 1 cut(s) 1109
PdmI GAANNNNTTC 2 cut(s) 150, 688
PfeI GAWTC 6 cut(s) 35, 653, 1251, 1528, 1822, 1853
PflMI CCANNNNNTGG 1 cut(s) 172
PkrI GCNGC 3 cut(s) 211, 820, 838
Ple19I CGATCG 1 cut(s) 999
PleI GAGTC 2 cut(s) 27, 127
PmeI GTTTAAAC 1 cut(s) 1776
PpsI GAGTC 2 cut(s) 27, 127
PpuMI RGGWCCY 1 cut(s) 568
PscI ACATGT 1 cut(s) 265
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 3 cut(s) 476, 1147, 1442
PspPI GGNCC 2 cut(s) 568, 1175
PspPPI RGGWCCY 1 cut(s) 568
PsuI RGATCY 4 cut(s) 413, 474, 1350, 1396
PvuI CGATCG 1 cut(s) 999
RsaI GTAC 3 cut(s) 96, 1070, 1676
RsaNI GTAC 3 cut(s) 95, 1069, 1675
RseI CAYNNNNRTG 1 cut(s) 221
SatI GCNGC 3 cut(s) 210, 819, 837
Sau3AI GATC 7 cut(s) 301, 413, 474, 996, 1350, 1396, 1833
Sau96I GGNCC 2 cut(s) 568, 1175
ScaI AGTACT 1 cut(s) 96
SchI GAGTC 2 cut(s) 28, 128
ScrFI CCNGG 1 cut(s) 846
SduI GDGCHC 3 cut(s) 784, 1054, 1234
SfaNI GCATC 2 cut(s) 907, 1009
SfcI CTRYAG 1 cut(s) 1310
SfuI TTCGAA 1 cut(s) 563
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 4 cut(s) 340, 1523, 1638, 1810
SmoI CTYRAG 4 cut(s) 340, 1523, 1638, 1810
SpeI ACTAGT 1 cut(s) 1409
SsiI CCGC 1 cut(s) 836
SspMI CTAG 4 cut(s) 39, 257, 735, 1410
StyD4I CCNGG 1 cut(s) 844
StyI CCWWGG 2 cut(s) 171, 606
TaaI ACNGT 4 cut(s) 707, 1674, 1685, 1781
TaiI ACGT 2 cut(s) 1497, 1774
TaqI TCGA 2 cut(s) 563, 975
TatI WGTACW 2 cut(s) 94, 1674
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 6 cut(s) 35, 653, 1251, 1528, 1822, 1853
TseFI GTSAC 1 cut(s) 1606
TseI GCWGC 2 cut(s) 209, 818
Tsp45I GTSAC 1 cut(s) 1606
TspDTI ATGAA 8 cut(s) 17, 1149, 1251, 1277, 1284, 1328, 1395, 1814
TspGWI ACGGA 1 cut(s) 1125
Van91I CCANNNNNTGG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 1230
VpaK11BI GGWCC 1 cut(s) 568
XapI RAATTY 9 cut(s) 77, 348, 382, 401, 407, 528, 1272, 1545, 1738
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 1 cut(s) 269
XmnI GAANNNNTTC 2 cut(s) 150, 688
XspI CTAG 4 cut(s) 39, 257, 735, 1410
ZrmI AGTACT 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 1618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.