Rmu_co8500389.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8500389.1
OG0003917
Physical Location & Seq
Forward (+)
1855 .. 2271
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8500389.1_g000002.1.cds

Sequence Viewer

Length: 417 bp
atgtggccggctctcctcgccgcagcagtcgccgcctccaccggactcctcgccaaaaaccacctcttcaagcccgccgccgagatcaccgactccgatccgcagaacgacgccgtctcggacgatgcgacgccgcagcgctccgtcgcttctgtttctcggtctccggtcctgccgtgggagagcgactgcgaggatcaaatgaaggaggggatctttaggttttcgagcgacggcgggagcgggaagaagaagaagaagaagaagaccaggtttcggttgaagaagaaagatgtggtggaggagcagaagaagaagagtgggaggagagctggtgtttgcttgaagaagaggaagactgtagcttccaaatgtggatcgtcttctgattccaaaggtttgatctctaaattgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

14.95

Weight (kDa)

9.94

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 243
AciI CCGC 8 cut(s) 21, 33, 75, 78, 101, 134, 237, 243
AclWI GGATC 4 cut(s) 92, 204, 221, 385
AcoI YGGCCR 1 cut(s) 5
AcyI GRCGYC 2 cut(s) 111, 131
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 2 cut(s) 178, 276
AgsI TTSAA 3 cut(s) 70, 283, 346
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 2 cut(s) 332, 365
AluI AGCT 2 cut(s) 332, 365
Alw26I GTCTC 2 cut(s) 121, 168
AlwI GGATC 4 cut(s) 92, 204, 221, 385
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 23, 136
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 169
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 169
BbsI GAAGAC 3 cut(s) 272, 362, 375
BbvI GCAGC 2 cut(s) 35, 148
BceAI ACGGC 3 cut(s) 98, 160, 250
BciT130I CCWGG 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 121, 168
BfmI CTRYAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 142
BisI GCNGC 6 cut(s) 21, 24, 33, 78, 134, 137
BlsI GCNGC 6 cut(s) 22, 25, 34, 79, 135, 138
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 115
BpiI GAAGAC 3 cut(s) 272, 362, 375
BsaHI GRCGYC 2 cut(s) 111, 131
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 176
BsaWI WCCGGW 2 cut(s) 41, 166
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 276
Bse118I RCCGGY 1 cut(s) 7
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 178, 276
BseRI GAGGAG 4 cut(s) 5, 38, 317, 340
BseXI GCAGC 2 cut(s) 35, 148
BshFI GGCC 1 cut(s) 7
BsiSI CCGG 3 cut(s) 8, 42, 167
BslI CCNNNNNNNGG 2 cut(s) 178, 276
BsmAI GTCTC 2 cut(s) 121, 168
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 1 cut(s) 7
Bso31I GGTCTC 1 cut(s) 168
Bsp143I GATC 6 cut(s) 84, 97, 196, 213, 377, 402
BspACI CCGC 8 cut(s) 21, 33, 75, 78, 101, 134, 237, 243
BspANI GGCC 1 cut(s) 7
BspPI GGATC 4 cut(s) 92, 204, 221, 385
BspTNI GGTCTC 1 cut(s) 168
BsrBI CCGCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 6 cut(s) 84, 97, 196, 213, 377, 402
BssNI GRCGYC 2 cut(s) 111, 131
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 3 cut(s) 71, 311, 344
BstACI GRCGYC 2 cut(s) 111, 131
BstC8I GCNNGC 2 cut(s) 9, 75
BstDSI CCRYGG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 6 cut(s) 87, 100, 199, 216, 380, 405
BstMAI GTCTC 2 cut(s) 121, 168
BstMBI GATC 6 cut(s) 84, 97, 196, 213, 377, 402
BstMWI GCNNNNNNNGC 3 cut(s) 17, 29, 32
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 35, 148
BstV2I GAAGAC 3 cut(s) 272, 362, 375
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 176
Cac8I GCNNGC 2 cut(s) 9, 75
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 169
CseI GACGC 2 cut(s) 119, 139
CsiI ACCWGGT 1 cut(s) 269
CviJI RGCY 5 cut(s) 7, 11, 73, 332, 365
CviKI_1 RGCY 5 cut(s) 7, 11, 73, 332, 365
DpnI GATC 6 cut(s) 86, 99, 198, 215, 379, 404
DpnII GATC 6 cut(s) 84, 97, 196, 213, 377, 402
EaeI YGGCCR 1 cut(s) 5
Eam1104I CTCTTC 3 cut(s) 71, 311, 344
EarI CTCTTC 3 cut(s) 71, 311, 344
Eco31I GGTCTC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 169
Eco47III AGCGCT 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 269
Esp3I CGTCTC 1 cut(s) 121
FauI CCCGC 3 cut(s) 82, 230, 236
Fnu4HI GCNGC 6 cut(s) 21, 24, 33, 78, 134, 137
Fsp4HI GCNGC 6 cut(s) 21, 24, 33, 78, 134, 137
GlaI GCGC 1 cut(s) 140
GluI GCNGC 6 cut(s) 21, 24, 33, 78, 134, 137
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 1 cut(s) 7
HapII CCGG 3 cut(s) 8, 42, 167
HgaI GACGC 2 cut(s) 119, 139
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 2 cut(s) 111, 131
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HinfI GANTC 3 cut(s) 45, 92, 389
HpaII CCGG 3 cut(s) 8, 42, 167
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 97, 121, 388
Hpy99I CGWCG 4 cut(s) 113, 133, 149, 236
HpyAV CCTTC 1 cut(s) 199
HpyCH4III ACNGT 1 cut(s) 361
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 29, 32
Hsp92I GRCGYC 2 cut(s) 111, 131
HspAI GCGC 1 cut(s) 139
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Kzo9I GATC 6 cut(s) 84, 97, 196, 213, 377, 402
LmnI GCTCC 3 cut(s) 146, 240, 304
LpnPI CCDG 7 cut(s) 21, 55, 180, 185, 256, 283, 318
Lsp1109I GCAGC 2 cut(s) 35, 148
LweI GCATC 1 cut(s) 115
MabI ACCWGGT 1 cut(s) 269
MalI GATC 6 cut(s) 86, 99, 198, 215, 379, 404
MbiI CCGCTC 1 cut(s) 243
MboI GATC 6 cut(s) 84, 97, 196, 213, 377, 402
MflI RGATCY 1 cut(s) 213
MluCI AATT 1 cut(s) 410
MlyI GAGTC 2 cut(s) 39, 86
MnlI CCTC 9 cut(s) 26, 46, 59, 74, 187, 202, 295, 318, 345
MroNI GCCGGC 1 cut(s) 7
MspI CCGG 3 cut(s) 8, 42, 167
MspR9I CCNGG 1 cut(s) 271
MvaI CCWGG 1 cut(s) 271
MwoI GCNNNNNNNGC 3 cut(s) 17, 29, 32
NaeI GCCGGC 1 cut(s) 9
NdeII GATC 6 cut(s) 84, 97, 196, 213, 377, 402
NgoMIV GCCGGC 1 cut(s) 7
NmeAIII GCCGAG 1 cut(s) 106
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PdiI GCCGGC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 389
PflFI GACNNNGTC 1 cut(s) 113
PkrI GCNGC 6 cut(s) 22, 25, 34, 79, 135, 138
PleI GAGTC 2 cut(s) 39, 86
PpsI GAGTC 2 cut(s) 39, 86
Psp6I CCWGG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 269
PspPI GGNCC 1 cut(s) 169
PsuI RGATCY 1 cut(s) 213
PsyI GACNNNGTC 1 cut(s) 113
SatI GCNGC 6 cut(s) 21, 24, 33, 78, 134, 137
Sau3AI GATC 6 cut(s) 84, 97, 196, 213, 377, 402
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 39, 86
ScrFI CCNGG 1 cut(s) 271
SetI ASST 6 cut(s) 66, 224, 275, 334, 367, 400
SexAI ACCWGGT 1 cut(s) 269
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 1 cut(s) 169
Sse9I AATT 1 cut(s) 410
SsiI CCGC 8 cut(s) 21, 33, 75, 78, 101, 134, 237, 243
StyD4I CCNGG 1 cut(s) 269
TaaI ACNGT 1 cut(s) 361
TaqI TCGA 1 cut(s) 227
TaqII GACCGA 1 cut(s) 150
TasI AATT 1 cut(s) 410
TauI GCSGC 4 cut(s) 23, 35, 80, 136
TfiI GAWTC 1 cut(s) 389
TseI GCWGC 2 cut(s) 23, 136
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 133
Tth111I GACNNNGTC 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.