MD04G1105400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003917
Physical Location & Seq
Reverse (-)
19351180 .. 19353826
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1105400.v1.1.491

Sequence Viewer

Length: 900 bp
ATGAGGAACACTGCCTGCACAGGCCCCTACAATTTCTCCGTCGCGTCGGCATTTCAGTCTCAGCCGCCTCCCTGGGAGACCAGCGACTTCGAGGAGCAACGAGAAGGGACGATCTTTAGGTTTTCGAGCGAGGCGGCGTCGGGTCGCGGCGGGAGTGGGACGCGGTTCCGGTTGAGGAAGAGAACCGGTTCGATCAAGAAGAAAGGGGTTGCTGCTGAGGCTGAGAGATCTAACGGTACAGAACAGAGGAGGACTGCAAGGAGAGGTGTTGGAGTTTGCTTGAAGAGGAGGAAGACTGGGAAGAATGTAGCTGCTACCAAATGTGGATCGTCTTCTTCCAAAGGTTACATTCAACATTATTTTGGTTGGGGACTTAATGTTGGGATCATGTACATGATGTCTGCTGGAAAAGTTGAGATCAATAAGCTGAATACAGCTATGGATGAGACTGCAAGAATTGTGCAGGAGTTAAAATCTGAGCTTCATAAAAGAAAATCACCGCAAAATCTGCAAGTTTCGGGCTCTGCAGGTGAAGCCAATACAACTAGTTGCACGCTTACTCAGCCAGAACTCAACAAGTCAAGTACAGAAAACAGAGGCCCCAGTCATATGAAAATGTCCAGTTTTACTGTATCTGAGGATGGTGAATGTGCTAGTAGTGTTCTTACAGAAGAGCAGGAGCCACATCCTGAGGTTATGGACATGGATCAGTTGGAAGCAGAACTCGAGTCTGAACTACAAAAACTTCCATGGTGTGCCATGGATACTCCTCGCCATGATGGATTGAGTAATCTGGGCCAGGCACACGGATTGGTGGTTTTGGTAATGAGTACTATTTGGGGAGGCATACAACATGCTCCGCTGGGTCAACACCGTATGGGACACCAATTAAAGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

32.88

Weight (kDa)

9.0

Isoelectric Point (pI)

57.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 518
AccII CGCG 3 cut(s) 44, 147, 163
AciI CCGC 7 cut(s) 65, 134, 147, 150, 163, 500, 860
AclWI GGATC 3 cut(s) 334, 392, 714
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 4 cut(s) 238, 392, 586, 832
AgeI ACCGGT 1 cut(s) 185
AgsI TTSAA 2 cut(s) 283, 353
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 2 cut(s) 71, 798
AluBI AGCT 4 cut(s) 311, 427, 437, 481
AluI AGCT 4 cut(s) 311, 427, 437, 481
Alw26I GTCTC 3 cut(s) 63, 71, 440
AlwI GGATC 3 cut(s) 334, 392, 714
Ama87I CYCGRG 1 cut(s) 725
AoxI GGCC 3 cut(s) 22, 598, 796
ApeKI GCWGC 2 cut(s) 212, 311
AsiGI ACCGGT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 23, 599, 796
AsuHPI GGTGA 3 cut(s) 489, 542, 656
AvaI CYCGRG 1 cut(s) 725
AxyI CCTNAGG 1 cut(s) 690
BanII GRGCYC 1 cut(s) 524
BbsI GAAGAC 2 cut(s) 299, 324
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 199, 298
BccI CCATC 2 cut(s) 635, 773
BciT130I CCWGG 2 cut(s) 73, 800
BciVI GTATCC 1 cut(s) 757
BcoDI GTCTC 3 cut(s) 63, 71, 440
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 2 cut(s) 546, 654
BfmI CTRYAG 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 518
BfuI GTATCC 1 cut(s) 757
BglII AGATCT 1 cut(s) 227
BisI GCNGC 5 cut(s) 65, 135, 148, 213, 312
BlsI GCNGC 5 cut(s) 66, 136, 149, 214, 313
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 2 cut(s) 73, 800
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 3 cut(s) 23, 599, 796
BmiI GGNNCC 4 cut(s) 25, 167, 601, 681
BmrFI CCNGG 2 cut(s) 73, 800
BmrI ACTGGG 2 cut(s) 306, 597
BmuI ACTGGG 2 cut(s) 306, 597
BpiI GAAGAC 2 cut(s) 299, 324
Bpu10I CCTNAGC 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 4 cut(s) 71, 72, 749, 759
BsaWI WCCGGW 2 cut(s) 168, 185
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 301, 603, 621
Bse21I CCTNAGG 1 cut(s) 690
BseBI CCWGG 2 cut(s) 73, 800
BseDI CCNNGG 4 cut(s) 71, 72, 749, 759
BseGI GGATG 3 cut(s) 448, 646, 685
BseMII CTCAG 7 cut(s) 74, 207, 213, 468, 575, 627, 681
BseNI ACTGG 3 cut(s) 301, 603, 621
BseRI GAGGAG 4 cut(s) 107, 262, 301, 759
BseXI GCAGC 2 cut(s) 199, 298
BseYI CCCAGC 1 cut(s) 862
BsgI GTGCAG 1 cut(s) 482
Bsh1236I CGCG 3 cut(s) 44, 147, 163
BshFI GGCC 3 cut(s) 24, 600, 798
BshTI ACCGGT 1 cut(s) 185
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 2 cut(s) 169, 186
BslFI GGGAC 4 cut(s) 121, 172, 384, 894
BsmAI GTCTC 3 cut(s) 63, 71, 440
BsmFI GGGAC 4 cut(s) 121, 172, 384, 894
BsnI GGCC 3 cut(s) 24, 600, 798
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 725
Bsp1286I GDGCHC 1 cut(s) 524
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
Bsp19I CCATGG 2 cut(s) 749, 759
BspACI CCGC 7 cut(s) 65, 134, 147, 150, 163, 500, 860
BspANI GGCC 3 cut(s) 24, 600, 798
BspCNI CTCAG 7 cut(s) 73, 208, 214, 469, 574, 628, 682
BspFNI CGCG 3 cut(s) 44, 147, 163
BspLI GGNNCC 4 cut(s) 25, 167, 601, 681
BspMAI CTGCAG 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 518
BspPI GGATC 3 cut(s) 334, 392, 714
BspQI GCTCTTC 1 cut(s) 666
BspTNI GGTCTC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 185
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 3 cut(s) 301, 603, 621
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 4 cut(s) 71, 72, 749, 759
BssMI GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 2 cut(s) 749, 759
Bst2UI CCWGG 2 cut(s) 73, 800
Bst4CI ACNGT 3 cut(s) 236, 631, 875
Bst6I CTCTTC 3 cut(s) 173, 278, 666
BstACI GRCGYC 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 508
BstAUI TGTACA 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 16, 554
BstDEI CTNAG 7 cut(s) 60, 216, 222, 477, 561, 636, 690
BstDSI CCRYGG 2 cut(s) 749, 759
BstF5I GGATG 3 cut(s) 448, 646, 685
BstFNI CGCG 3 cut(s) 44, 147, 163
BstKTI GATC 7 cut(s) 114, 195, 230, 329, 387, 420, 709
BstMAI GTCTC 3 cut(s) 63, 71, 440
BstMBI GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
BstMWI GCNNNNNNNGC 4 cut(s) 218, 508, 533, 562
BstNI CCWGG 2 cut(s) 73, 800
BstNSI RCATGY 1 cut(s) 857
BstSCI CCNGG 2 cut(s) 71, 798
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 3 cut(s) 44, 147, 163
BstV1I GCAGC 2 cut(s) 199, 298
BstV2I GAAGAC 2 cut(s) 299, 324
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
Bsu36I CCTNAGG 1 cut(s) 690
BsuI GTATCC 1 cut(s) 757
BsuRI GGCC 3 cut(s) 24, 600, 798
BtgI CCRYGG 2 cut(s) 749, 759
BtsCI GGATG 3 cut(s) 448, 646, 685
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 9
BveI ACCTGC 1 cut(s) 518
Cac8I GCNNGC 2 cut(s) 16, 554
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 23, 599, 796
CseI GACGC 3 cut(s) 33, 126, 169
Csp6I GTAC 4 cut(s) 237, 391, 585, 831
CspAI ACCGGT 1 cut(s) 185
CspCI CAANNNNNGTGG 1 cut(s) 875
CviAII CATG 7 cut(s) 388, 394, 703, 750, 760, 776, 854
CviQI GTAC 4 cut(s) 237, 391, 585, 831
DdeI CTNAG 7 cut(s) 60, 216, 222, 477, 561, 636, 690
DpnI GATC 7 cut(s) 113, 194, 229, 328, 386, 419, 708
DpnII GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
Eam1104I CTCTTC 3 cut(s) 173, 278, 666
EarI CTCTTC 3 cut(s) 173, 278, 666
Eco130I CCWWGG 2 cut(s) 749, 759
Eco24I GRGCYC 1 cut(s) 524
Eco31I GGTCTC 1 cut(s) 71
Eco81I CCTNAGG 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 725
EcoO109I RGGNCCY 2 cut(s) 23, 599
EcoRII CCWGG 2 cut(s) 71, 798
EcoT14I CCWWGG 2 cut(s) 749, 759
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 2 cut(s) 749, 759
FaeI CATG 7 cut(s) 391, 397, 706, 753, 763, 779, 857
FaqI GGGAC 4 cut(s) 121, 172, 384, 894
FatI CATG 7 cut(s) 387, 393, 702, 749, 759, 775, 853
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 609
Fnu4HI GCNGC 5 cut(s) 65, 135, 148, 213, 312
FokI GGATG 3 cut(s) 455, 653, 672
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 5 cut(s) 65, 135, 148, 213, 312
FspBI CTAG 2 cut(s) 546, 654
GluI GCNGC 5 cut(s) 65, 135, 148, 213, 312
GsaI CCCAGC 1 cut(s) 866
HaeIII GGCC 3 cut(s) 24, 600, 798
HapII CCGG 2 cut(s) 169, 186
HgaI GACGC 3 cut(s) 33, 126, 169
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 7 cut(s) 391, 397, 706, 753, 763, 779, 857
HincII GTYRAC 1 cut(s) 869
HindII GTYRAC 1 cut(s) 869
HinfI GANTC 1 cut(s) 728
HpaII CCGG 2 cut(s) 169, 186
HphI GGTGA 3 cut(s) 489, 542, 656
Hpy166II GTNNAC 1 cut(s) 869
Hpy188I TCNGA 3 cut(s) 478, 637, 733
Hpy188III TCNNGA 2 cut(s) 196, 689
Hpy8I GTNNAC 1 cut(s) 869
Hpy99I CGWCG 3 cut(s) 44, 49, 142
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 3 cut(s) 236, 631, 875
HpyCH4V TGCA 7 cut(s) 18, 257, 452, 463, 511, 527, 552
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 508, 533, 562
HpyF3I CTNAG 7 cut(s) 60, 216, 222, 477, 561, 636, 690
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 7 cut(s) 391, 397, 706, 753, 763, 779, 857
Kzo9I GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 94, 679, 862
Lsp1109I GCAGC 2 cut(s) 199, 298
MaeI CTAG 2 cut(s) 546, 654
MaeIII GTNAC 1 cut(s) 344
MalI GATC 7 cut(s) 113, 194, 229, 328, 386, 419, 708
MboI GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
MboII GAAGA 8 cut(s) 190, 211, 295, 304, 313, 324, 327, 683
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 3 cut(s) 31, 456, 887
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 2 cut(s) 250, 693
MroXI GAANNNNTTC 1 cut(s) 187
MseI TTAA 3 cut(s) 375, 470, 890
MslI CAYNNNNRTG 1 cut(s) 392
MspA1I CMGCKG 1 cut(s) 862
MspI CCGG 2 cut(s) 169, 186
MspR9I CCNGG 2 cut(s) 73, 800
MvaI CCWGG 2 cut(s) 73, 800
MvnI CGCG 3 cut(s) 44, 147, 163
MwoI GCNNNNNNNGC 4 cut(s) 218, 508, 533, 562
NcoI CCATGG 2 cut(s) 749, 759
NdeI CATATG 1 cut(s) 609
NdeII GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
NlaIII CATG 7 cut(s) 391, 397, 706, 753, 763, 779, 857
NlaIV GGNNCC 4 cut(s) 25, 167, 601, 681
NspI RCATGY 1 cut(s) 857
PaeR7I CTCGAG 1 cut(s) 725
PaqCI CACCTGC 1 cut(s) 518
PasI CCCWGGG 1 cut(s) 72
PciSI GCTCTTC 1 cut(s) 666
PdmI GAANNNNTTC 1 cut(s) 187
PinAI ACCGGT 1 cut(s) 185
PkrI GCNGC 5 cut(s) 66, 136, 149, 214, 313
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
Psp6I CCWGG 2 cut(s) 71, 798
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 2 cut(s) 71, 798
PspN4I GGNNCC 4 cut(s) 25, 167, 601, 681
PspPI GGNCC 3 cut(s) 23, 599, 796
PspXI VCTCGAGB 1 cut(s) 725
PstI CTGCAG 1 cut(s) 529
PsuI RGATCY 1 cut(s) 227
RsaI GTAC 4 cut(s) 238, 392, 586, 832
RsaNI GTAC 4 cut(s) 237, 391, 585, 831
RseI CAYNNNNRTG 1 cut(s) 392
SapI GCTCTTC 1 cut(s) 666
SaqAI TTAA 3 cut(s) 375, 470, 890
SatI GCNGC 5 cut(s) 65, 135, 148, 213, 312
Sau3AI GATC 7 cut(s) 111, 192, 227, 326, 384, 417, 706
Sau96I GGNCC 3 cut(s) 23, 599, 796
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 73, 800
SduI GDGCHC 1 cut(s) 524
SetI ASST 9 cut(s) 122, 268, 313, 346, 429, 439, 483, 532, 696
SfcI CTRYAG 1 cut(s) 525
Sfr274I CTCGAG 1 cut(s) 725
SlaI CTCGAG 1 cut(s) 725
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 3 cut(s) 31, 456, 887
SsiI CCGC 7 cut(s) 65, 134, 147, 150, 163, 500, 860
SspMI CTAG 2 cut(s) 546, 654
StyD4I CCNGG 2 cut(s) 71, 798
StyI CCWWGG 2 cut(s) 749, 759
TaaI ACNGT 3 cut(s) 236, 631, 875
TaqI TCGA 4 cut(s) 90, 125, 191, 726
TasI AATT 3 cut(s) 31, 456, 887
TatI WGTACW 3 cut(s) 390, 584, 830
TauI GCSGC 3 cut(s) 67, 137, 150
Tru1I TTAA 3 cut(s) 375, 470, 890
Tru9I TTAA 3 cut(s) 375, 470, 890
TscAI CASTG 1 cut(s) 16
TseI GCWGC 2 cut(s) 212, 311
TspDTI ATGAA 2 cut(s) 473, 626
TspGWI ACGGA 2 cut(s) 28, 822
TspRI CASTG 1 cut(s) 16
XceI RCATGY 1 cut(s) 857
XhoI CTCGAG 1 cut(s) 725
XmnI GAANNNNTTC 1 cut(s) 187
XspI CTAG 2 cut(s) 546, 654
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.