MD05G1051300.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003809
Physical Location & Seq
Forward (+)
8879203 .. 8889738
10536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1051300.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGGCTTCTTCAGAGATAGAGGTGGTGTCTTCGGACTCAAAAACCCAGGAAAACCCTCCCGAGGTTCCCGTGTTCGACGTTTTCTCTGCTTCTGCTTATGGCGATTTTCAGAAACTCAGAAAGTTCGTCGAGCAAGACGGTGCCTCTCTCTCCAAGCCAGACGTCCATGGCTACTACGCCCTCCACTGGGCTGCTCTCAATAACTTCCCCGATATTGCCCACTACATAATTCAGCATGGTGGAGATGTCAATGCGACTGAAAATGTACATCAGACAGCATTGCATTGGGCAGCCGTGAAGGGTGCAATTGCGGCGGCTGATGTGCTCTTGCAGAATGGAGCTAGAGTTGAGGCAGCTGATGTACATGGCTACCGGGCAGTTCACGTTGCTGCTCAATATGGGCAAACATCTTTTCTGAATCACATTGTTGCAAGATATCATGCTGATTTTGATGCACCTGATAATGAGGGGAGGAGTCCTCTTCATTGGGCCGCATATAAAGGTTTTGCAGATACAGTTAGATTACTTCTGTTTAGAGATGCATGCCAAGGGAGGCAAGATAAAGAAGGTTGTACCCCTTTGCACTGGGCAGCTTTAAGTGGGAACGTGGAGGCTTGCACAGTGCTTGTCTATGCTGGCACTAAGCAGGAATTAACTGTGAAAGACAATGCTGGTTTTACGCCTGTTCAGATTGCATCTGATAAAGGTCATCAGCGTGTCGCTCTTTTCCTTGCTAACCAGCAGCGGGCTCATAGCAACTGCTGGAGAGACAAATTTTGCAGTGGGAAGATGGCGGATATTGGTTACGCACCTGTCTTGTTGTGTATAATAGTCTTTCAGACAGTTCTCTTTATGAATTCTGTCCTTGCAGCTCCTAATCTGACAAAGGTTACTGCTGCTGTCGGTATCTGGGGATGGACTGCCATTTCATTAGCAGTGGGTTCCTTGATTATGTTCTACAGGTGCAGCAGTAAAGATCCAGGTTACATCAAAAGAGCAGGGGATTTAAGCAATCGTATAAATACAGAGGATCCTTTGTTGAACGTTGATTTGAACAATTCCGCTGTATGGATGGGAAATTGGTCTCAACTTTGTCCCACTTGCAAGATAATTAGACCTGTTCGCTCTAAACACTGCCCCTCATGTAACCGATGCGTAGAACAGTTTGACCATCATTGTCCATGGATATCTAATTGTGTTGGAAAGAGAAACAAGCGAGATTTCTTTATACTTATCTGCCTGGGAACTTTGACGTCACTCATTTCTACTGCTGTTGCAGTTCAAAGGATTTGGGGTGCATTGCCAGGACTGCGAACAGAAGAATCATGGATTCGTCGTGTGGTAGTTCAATATCCTAGTATTGTTGCTTTTCTGTTGGTGGACGTAATTATTTTAATTGCTGTTACTACCCTGACAACGGCGCAGGCAACTCAGATAGCTCGAAATATCACCACCAACGAATTGGCGAATGCTATTCGTTATGGGTATCTTCGTGGTCCAGATGGGCGGTTCCGAAACCCATATAACCATGGGTGCAGGAAAAATTGTTCTGATTTTCTCATTCAGGGCCACACAGATGATGAAATGGCTTGGCCTCCATTGCAGCAGGTTGCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 DHHC

Protein Analysis

540

Amino Acids

59.54

Weight (kDa)

7.85

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 30 - 119 1e-11 Ankyrin repeats (3 copies)
Ank_3 PF13606 56 - 85 8.8e-06 Ankyrin repeat
Ank_2 PF12796 128 - 215 3.6e-17 Ankyrin repeats (3 copies)
Ank_2 PF12796 186 - 247 1.5e-08 Ankyrin repeats (3 copies)
DHHC PF01529 363 - 490 3.6e-33 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 165, 1256
Acc36I ACCTGC 1 cut(s) 1597
AccB1I GGYRCC 1 cut(s) 140
AciI CCGC 7 cut(s) 311, 314, 492, 745, 794, 1062, 1507
AclI AACGTT 1 cut(s) 1044
AclWI GGATC 3 cut(s) 971, 1025, 1038
AcsI RAATTY 2 cut(s) 773, 856
AcyI GRCGYC 2 cut(s) 162, 1253
AfaI GTAC 3 cut(s) 267, 363, 574
AfiI CCNNNNNNNGG 6 cut(s) 61, 186, 187, 745, 1068, 1417
AgsI TTSAA 4 cut(s) 1042, 1054, 1283, 1349
AjnI CCWGG 4 cut(s) 45, 979, 1239, 1303
AloI GAACNNNNNNTCC 2 cut(s) 1531, 1563
AluBI AGCT 5 cut(s) 341, 356, 593, 872, 1439
AluI AGCT 5 cut(s) 341, 356, 593, 872, 1439
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 2 cut(s) 762, 1089
AlwI GGATC 3 cut(s) 971, 1025, 1038
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 3 cut(s) 489, 1567, 1592
ApoI RAATTY 2 cut(s) 773, 856
AspLEI GCGC 1 cut(s) 1423
AspS9I GGNCC 3 cut(s) 489, 1496, 1567
AsuC2I CCSGG 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 1441
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 1496
BamHI GGATCC 1 cut(s) 1030
BanI GGYRCC 1 cut(s) 140
BanII GRGCYC 1 cut(s) 751
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 1 cut(s) 327
BccI CCATC 5 cut(s) 784, 909, 1066, 1179, 1496
BceAI ACGGC 2 cut(s) 278, 1434
BciT130I CCWGG 4 cut(s) 47, 981, 1241, 1305
BcnI CCSGG 1 cut(s) 374
BcoDI GTCTC 2 cut(s) 762, 1089
BfaI CTAG 2 cut(s) 342, 1356
BfmI CTRYAG 1 cut(s) 958
BfuAI ACCTGC 1 cut(s) 1597
Bme1390I CCNGG 5 cut(s) 47, 374, 981, 1241, 1305
Bme18I GGWCC 1 cut(s) 1496
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 3 cut(s) 489, 1496, 1567
BmiI GGNNCC 5 cut(s) 66, 142, 943, 1032, 1511
BmrFI CCNGG 5 cut(s) 47, 374, 981, 1241, 1305
BmrI ACTGGG 2 cut(s) 196, 595
BmsI GCATC 4 cut(s) 442, 529, 704, 1142
BmuI ACTGGG 2 cut(s) 196, 595
BpiI GAAGAC 1 cut(s) 21
BplI GAGNNNNNCTC 2 cut(s) 463, 495
BpmI CTGGAG 1 cut(s) 784
BpuMI CCSGG 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 162, 1253
BsaI GGTCTC 1 cut(s) 1089
BsaJI CCNNGG 7 cut(s) 45, 60, 166, 547, 1181, 1240, 1528
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 186, 187, 745, 1068, 1417
Bse1I ACTGG 3 cut(s) 191, 590, 1614
Bse3DI GCAATG 3 cut(s) 278, 1298, 1598
BseBI CCWGG 4 cut(s) 47, 981, 1241, 1305
BseDI CCNNGG 7 cut(s) 45, 60, 166, 547, 1181, 1240, 1528
BseGI GGATG 2 cut(s) 920, 1077
BseLI CCNNNNNNNGG 6 cut(s) 61, 186, 187, 745, 1068, 1417
BseMI GCAATG 3 cut(s) 278, 1298, 1598
BseMII CTCAG 2 cut(s) 130, 1445
BseNI ACTGG 3 cut(s) 191, 590, 1614
BseRI GAGGAG 1 cut(s) 487
BsgI GTGCAG 2 cut(s) 985, 1555
BshFI GGCC 3 cut(s) 491, 1569, 1594
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 1080
BslI CCNNNNNNNGG 6 cut(s) 61, 186, 187, 745, 1068, 1417
BsmAI GTCTC 2 cut(s) 762, 1089
BsmFI GGGAC 1 cut(s) 1080
BsmI GAATGC 1 cut(s) 1474
BsnI GGCC 3 cut(s) 491, 1569, 1594
Bso31I GGTCTC 1 cut(s) 1089
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 327, 751
Bsp1407I TGTACA 2 cut(s) 265, 361
Bsp143I GATC 2 cut(s) 976, 1030
Bsp19I CCATGG 3 cut(s) 166, 1181, 1528
BspACI CCGC 7 cut(s) 311, 314, 492, 745, 794, 1062, 1507
BspANI GGCC 3 cut(s) 491, 1569, 1594
BspCNI CTCAG 2 cut(s) 129, 1444
BspLI GGNNCC 5 cut(s) 66, 142, 943, 1032, 1511
BspMI ACCTGC 1 cut(s) 1597
BspPI GGATC 3 cut(s) 971, 1025, 1038
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 1089
BsrDI GCAATG 3 cut(s) 278, 1298, 1598
BsrGI TGTACA 2 cut(s) 265, 361
BsrI ACTGG 3 cut(s) 191, 590, 1614
BssECI CCNNGG 7 cut(s) 45, 60, 166, 547, 1181, 1240, 1528
BssMI GATC 2 cut(s) 976, 1030
BssNI GRCGYC 2 cut(s) 162, 1253
BssT1I CCWWGG 4 cut(s) 166, 547, 1181, 1528
Bst2UI CCWGG 4 cut(s) 47, 981, 1241, 1305
Bst4CI ACNGT 6 cut(s) 140, 517, 622, 658, 844, 1164
Bst6I CTCTTC 1 cut(s) 486
BstACI GRCGYC 2 cut(s) 162, 1253
BstAUI TGTACA 2 cut(s) 265, 361
BstC8I GCNNGC 5 cut(s) 544, 616, 637, 747, 1425
BstDEI CTNAG 3 cut(s) 116, 642, 1431
BstDSI CCRYGG 3 cut(s) 166, 1181, 1528
BstF5I GGATG 2 cut(s) 920, 1077
BstHHI GCGC 1 cut(s) 1423
BstKTI GATC 2 cut(s) 979, 1033
BstMAI GTCTC 2 cut(s) 762, 1089
BstMBI GATC 2 cut(s) 976, 1030
BstMWI GCNNNNNNNGC 3 cut(s) 311, 1309, 1600
BstNI CCWGG 4 cut(s) 47, 981, 1241, 1305
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 5 cut(s) 45, 372, 979, 1239, 1303
BstSFI CTRYAG 1 cut(s) 958
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 2 cut(s) 976, 1030
BstXI CCANNNNNNTGG 1 cut(s) 1462
BstYI RGATCY 2 cut(s) 976, 1030
BsuRI GGCC 3 cut(s) 491, 1569, 1594
BtgI CCRYGG 3 cut(s) 166, 1181, 1528
BtsCI GGATG 2 cut(s) 920, 1077
BtsI GCAGTG 3 cut(s) 787, 942, 1132
BtsIMutI CAGTG 6 cut(s) 184, 583, 627, 787, 942, 1132
BveI ACCTGC 1 cut(s) 1597
Cac8I GCNNGC 5 cut(s) 544, 616, 637, 747, 1425
CfoI GCGC 1 cut(s) 1423
Cfr13I GGNCC 3 cut(s) 489, 1496, 1567
Csp6I GTAC 3 cut(s) 266, 362, 573
CviAII CATG 9 cut(s) 167, 236, 365, 440, 543, 1143, 1182, 1326, 1529
CviQI GTAC 3 cut(s) 266, 362, 573
DdeI CTNAG 3 cut(s) 116, 642, 1431
DpnI GATC 2 cut(s) 978, 1032
DpnII GATC 2 cut(s) 976, 1030
Eam1104I CTCTTC 1 cut(s) 486
EarI CTCTTC 1 cut(s) 486
EciI GGCGGA 1 cut(s) 809
Eco130I CCWWGG 4 cut(s) 166, 547, 1181, 1528
Eco24I GRGCYC 1 cut(s) 751
Eco31I GGTCTC 1 cut(s) 1089
Eco32I GATATC 2 cut(s) 437, 1188
Eco47I GGWCC 1 cut(s) 1496
Eco88I CYCGRG 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 856
EcoRII CCWGG 4 cut(s) 45, 979, 1239, 1303
EcoRV GATATC 2 cut(s) 437, 1188
EcoT14I CCWWGG 4 cut(s) 166, 547, 1181, 1528
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 4 cut(s) 166, 547, 1181, 1528
FaeI CATG 9 cut(s) 170, 239, 368, 443, 546, 1146, 1185, 1329, 1532
FaqI GGGAC 1 cut(s) 1080
FatI CATG 9 cut(s) 166, 235, 364, 439, 542, 1142, 1181, 1325, 1528
FauI CCCGC 1 cut(s) 738
FokI GGATG 2 cut(s) 927, 1084
FriOI GRGCYC 1 cut(s) 751
FspBI CTAG 2 cut(s) 342, 1356
GlaI GCGC 1 cut(s) 1422
GsuI CTGGAG 1 cut(s) 784
HaeIII GGCC 3 cut(s) 491, 1569, 1594
HapII CCGG 1 cut(s) 373
HhaI GCGC 1 cut(s) 1423
Hin1I GRCGYC 2 cut(s) 162, 1253
Hin1II CATG 9 cut(s) 170, 239, 368, 443, 546, 1146, 1185, 1329, 1532
Hin6I GCGC 1 cut(s) 1421
HinP1I GCGC 1 cut(s) 1421
HinfI GANTC 5 cut(s) 35, 418, 475, 1322, 1330
HpaII CCGG 1 cut(s) 373
HphI GGTGA 1 cut(s) 1441
Hpy166II GTNNAC 2 cut(s) 382, 1381
Hpy188III TCNNGA 2 cut(s) 59, 1499
Hpy8I GTNNAC 2 cut(s) 382, 1381
Hpy99I CGWCG 3 cut(s) 80, 131, 1338
HpyAV CCTTC 2 cut(s) 292, 560
HpyCH4III ACNGT 6 cut(s) 140, 517, 622, 658, 844, 1164
HpyCH4IV ACGT 7 cut(s) 78, 162, 384, 606, 1044, 1253, 1383
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 1309, 1600
HpyF3I CTNAG 3 cut(s) 116, 642, 1431
HpySE526I ACGT 7 cut(s) 78, 162, 384, 606, 1044, 1253, 1383
Hsp92I GRCGYC 2 cut(s) 162, 1253
Hsp92II CATG 9 cut(s) 170, 239, 368, 443, 546, 1146, 1185, 1329, 1532
HspAI GCGC 1 cut(s) 1421
Kzo9I GATC 2 cut(s) 976, 1030
LmnI GCTCC 2 cut(s) 338, 877
LweI GCATC 4 cut(s) 442, 529, 704, 1142
MaeI CTAG 2 cut(s) 342, 1356
MaeII ACGT 7 cut(s) 78, 162, 384, 606, 1044, 1253, 1383
MaeIII GTNAC 6 cut(s) 803, 889, 983, 1145, 1254, 1402
MalI GATC 2 cut(s) 978, 1032
MboI GATC 2 cut(s) 976, 1030
MboII GAAGA 5 cut(s) 21, 473, 799, 1331, 1481
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 2 cut(s) 976, 1030
MhlI GDGCHC 2 cut(s) 327, 751
MlyI GAGTC 2 cut(s) 29, 484
MmeI TCCRAC 1 cut(s) 1180
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 4 cut(s) 596, 653, 1007, 1394
MslI CAYNNNNRTG 2 cut(s) 714, 1575
MspA1I CMGCKG 3 cut(s) 356, 745, 1064
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 5 cut(s) 47, 374, 981, 1241, 1305
MunI CAATTG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 1474
MvaI CCWGG 4 cut(s) 47, 981, 1241, 1305
MwoI GCNNNNNNNGC 3 cut(s) 311, 1309, 1600
NciI CCSGG 1 cut(s) 374
NcoI CCATGG 3 cut(s) 166, 1181, 1528
NdeII GATC 2 cut(s) 976, 1030
NlaIII CATG 9 cut(s) 170, 239, 368, 443, 546, 1146, 1185, 1329, 1532
NlaIV GGNNCC 5 cut(s) 66, 142, 943, 1032, 1511
NmuCI GTSAC 1 cut(s) 1254
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 546
PaeI GCATGC 1 cut(s) 546
PctI GAATGC 1 cut(s) 1474
PfeI GAWTC 3 cut(s) 418, 1322, 1330
PleI GAGTC 2 cut(s) 29, 483
PpsI GAGTC 2 cut(s) 29, 483
Psp1406I AACGTT 1 cut(s) 1044
Psp6I CCWGG 4 cut(s) 45, 979, 1239, 1303
PspGI CCWGG 4 cut(s) 45, 979, 1239, 1303
PspN4I GGNNCC 5 cut(s) 66, 142, 943, 1032, 1511
PspPI GGNCC 3 cut(s) 489, 1496, 1567
PsuI RGATCY 2 cut(s) 976, 1030
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 3 cut(s) 267, 363, 574
RsaNI GTAC 3 cut(s) 266, 362, 573
RseI CAYNNNNRTG 2 cut(s) 714, 1575
SaqAI TTAA 4 cut(s) 596, 653, 1007, 1394
Sau3AI GATC 2 cut(s) 976, 1030
Sau96I GGNCC 3 cut(s) 489, 1496, 1567
SchI GAGTC 2 cut(s) 29, 484
ScrFI CCNGG 5 cut(s) 47, 374, 981, 1241, 1305
SduI GDGCHC 2 cut(s) 327, 751
SfaNI GCATC 4 cut(s) 442, 529, 704, 1142
SfcI CTRYAG 1 cut(s) 958
SinI GGWCC 1 cut(s) 1496
SmiMI CAYNNNNRTG 2 cut(s) 714, 1575
SphI GCATGC 1 cut(s) 546
SsiI CCGC 7 cut(s) 311, 314, 492, 745, 794, 1062, 1507
SspMI CTAG 2 cut(s) 342, 1356
StyD4I CCNGG 5 cut(s) 45, 372, 979, 1239, 1303
StyI CCWWGG 4 cut(s) 166, 547, 1181, 1528
TaaI ACNGT 6 cut(s) 140, 517, 622, 658, 844, 1164
TaiI ACGT 7 cut(s) 81, 165, 387, 609, 1047, 1256, 1386
TaqI TCGA 3 cut(s) 75, 129, 1441
TatI WGTACW 2 cut(s) 265, 361
TauI GCSGC 3 cut(s) 314, 317, 494
TfiI GAWTC 3 cut(s) 418, 1322, 1330
Tru1I TTAA 4 cut(s) 596, 653, 1007, 1394
Tru9I TTAA 4 cut(s) 596, 653, 1007, 1394
TscAI CASTG 6 cut(s) 191, 590, 627, 787, 942, 1139
TseFI GTSAC 1 cut(s) 1254
Tsp45I GTSAC 1 cut(s) 1254
TspDTI ATGAA 4 cut(s) 473, 869, 918, 1596
TspRI CASTG 6 cut(s) 191, 590, 627, 787, 942, 1139
VpaK11BI GGWCC 1 cut(s) 1496
XapI RAATTY 2 cut(s) 773, 856
XceI RCATGY 1 cut(s) 546
XcmI CCANNNNNNNNNTGG 1 cut(s) 1459
XspI CTAG 2 cut(s) 342, 1356
ZraI GACGTC 2 cut(s) 163, 1254
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.