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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 255 |
| AciI | CCGC | 1 cut(s) | 188 |
| AfeI | AGCGCT | 1 cut(s) | 156 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 125 |
| AflII | CTTAAG | 1 cut(s) | 266 |
| AgsI | TTSAA | 4 cut(s) | 88, 141, 220, 278 |
| AluBI | AGCT | 3 cut(s) | 137, 271, 304 |
| AluI | AGCT | 3 cut(s) | 137, 271, 304 |
| Alw26I | GTCTC | 1 cut(s) | 123 |
| Ama87I | CYCGRG | 1 cut(s) | 293 |
| Aor51HI | AGCGCT | 1 cut(s) | 156 |
| ApeKI | GCWGC | 2 cut(s) | 18, 175 |
| AspLEI | GCGC | 2 cut(s) | 157, 257 |
| AvaI | CYCGRG | 1 cut(s) | 293 |
| BanII | GRGCYC | 1 cut(s) | 111 |
| BbsI | GAAGAC | 3 cut(s) | 202, 254, 285 |
| BbvI | GCAGC | 2 cut(s) | 30, 162 |
| BcoDI | GTCTC | 1 cut(s) | 123 |
| BfoI | RGCGCY | 1 cut(s) | 158 |
| BfrI | CTTAAG | 1 cut(s) | 266 |
| BisI | GCNGC | 3 cut(s) | 19, 176, 189 |
| BlsI | GCNGC | 3 cut(s) | 20, 177, 190 |
| BmeT110I | CYCGRG | 1 cut(s) | 293 |
| BpiI | GAAGAC | 3 cut(s) | 202, 254, 285 |
| BpuEI | CTTGAG | 1 cut(s) | 143 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 125 |
| Bse3DI | GCAATG | 1 cut(s) | 22 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 125 |
| BseMI | GCAATG | 1 cut(s) | 22 |
| BseRI | GAGGAG | 2 cut(s) | 50, 53 |
| BseXI | GCAGC | 2 cut(s) | 30, 162 |
| BseYI | CCCAGC | 1 cut(s) | 178 |
| Bsh1236I | CGCG | 1 cut(s) | 255 |
| BsiHKCI | CYCGRG | 1 cut(s) | 293 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 125 |
| BsmAI | GTCTC | 1 cut(s) | 123 |
| BsoBI | CYCGRG | 1 cut(s) | 293 |
| Bsp1286I | GDGCHC | 1 cut(s) | 111 |
| BspACI | CCGC | 1 cut(s) | 188 |
| BspFNI | CGCG | 1 cut(s) | 255 |
| BspQI | GCTCTTC | 1 cut(s) | 240 |
| BspTI | CTTAAG | 1 cut(s) | 266 |
| BsrDI | GCAATG | 1 cut(s) | 22 |
| Bst6I | CTCTTC | 2 cut(s) | 233, 240 |
| BstAFI | CTTAAG | 1 cut(s) | 266 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 24 |
| BstFNI | CGCG | 1 cut(s) | 255 |
| BstH2I | RGCGCY | 1 cut(s) | 158 |
| BstHHI | GCGC | 2 cut(s) | 157, 257 |
| BstMAI | GTCTC | 1 cut(s) | 123 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 24, 161 |
| BstUI | CGCG | 1 cut(s) | 255 |
| BstV1I | GCAGC | 2 cut(s) | 30, 162 |
| BstV2I | GAAGAC | 3 cut(s) | 202, 254, 285 |
| BtsI | GCAGTG | 1 cut(s) | 171 |
| BtsIMutI | CAGTG | 1 cut(s) | 171 |
| CfoI | GCGC | 2 cut(s) | 157, 257 |
| CviJI | RGCY | 8 cut(s) | 21, 78, 109, 137, 191, 201, 271, 304 |
| CviKI_1 | RGCY | 8 cut(s) | 21, 78, 109, 137, 191, 201, 271, 304 |
| Eam1104I | CTCTTC | 2 cut(s) | 233, 240 |
| EarI | CTCTTC | 2 cut(s) | 233, 240 |
| Eco24I | GRGCYC | 1 cut(s) | 111 |
| Eco47III | AGCGCT | 1 cut(s) | 156 |
| Eco88I | CYCGRG | 1 cut(s) | 293 |
| EcoT38I | GRGCYC | 1 cut(s) | 111 |
| FaiI | YATR | 1 cut(s) | 226 |
| Fnu4HI | GCNGC | 3 cut(s) | 19, 176, 189 |
| FriOI | GRGCYC | 1 cut(s) | 111 |
| Fsp4HI | GCNGC | 3 cut(s) | 19, 176, 189 |
| GlaI | GCGC | 2 cut(s) | 156, 256 |
| GluI | GCNGC | 3 cut(s) | 19, 176, 189 |
| GsaI | CCCAGC | 1 cut(s) | 182 |
| HaeII | RGCGCY | 1 cut(s) | 158 |
| HhaI | GCGC | 2 cut(s) | 157, 257 |
| Hin6I | GCGC | 2 cut(s) | 155, 255 |
| HinP1I | GCGC | 2 cut(s) | 155, 255 |
| HindIII | AAGCTT | 1 cut(s) | 135 |
| Hpy188I | TCNGA | 1 cut(s) | 15 |
| Hpy188III | TCNNGA | 1 cut(s) | 220 |
| Hpy99I | CGWCG | 1 cut(s) | 287 |
| HpyCH4V | TGCA | 1 cut(s) | 40 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 24, 161 |
| HspAI | GCGC | 2 cut(s) | 155, 255 |
| LguI | GCTCTTC | 1 cut(s) | 240 |
| LmnI | GCTCC | 1 cut(s) | 15 |
| LpnPI | CCDG | 1 cut(s) | 164 |
| Lsp1109I | GCAGC | 2 cut(s) | 30, 162 |
| MboII | GAAGA | 7 cut(s) | 202, 244, 247, 250, 254, 257, 290 |
| MhlI | GDGCHC | 1 cut(s) | 111 |
| MluCI | AATT | 2 cut(s) | 54, 88 |
| MnlI | CCTC | 5 cut(s) | 71, 74, 89, 121, 289 |
| MseI | TTAA | 2 cut(s) | 267, 312 |
| MspCI | CTTAAG | 1 cut(s) | 266 |
| MvnI | CGCG | 1 cut(s) | 255 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 24, 161 |
| PaeR7I | CTCGAG | 1 cut(s) | 293 |
| PciSI | GCTCTTC | 1 cut(s) | 240 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 291 |
| PkrI | GCNGC | 3 cut(s) | 20, 177, 190 |
| PspFI | CCCAGC | 1 cut(s) | 178 |
| PspXI | VCTCGAGB | 1 cut(s) | 293 |
| SapI | GCTCTTC | 1 cut(s) | 240 |
| SaqAI | TTAA | 2 cut(s) | 267, 312 |
| SatI | GCNGC | 3 cut(s) | 19, 176, 189 |
| SduI | GDGCHC | 1 cut(s) | 111 |
| SetI | ASST | 5 cut(s) | 97, 139, 273, 300, 306 |
| Sfr274I | CTCGAG | 1 cut(s) | 293 |
| SlaI | CTCGAG | 1 cut(s) | 293 |
| SmlI | CTYRAG | 3 cut(s) | 158, 266, 293 |
| SmoI | CTYRAG | 3 cut(s) | 158, 266, 293 |
| Sse9I | AATT | 2 cut(s) | 54, 88 |
| SsiI | CCGC | 1 cut(s) | 188 |
| TaqI | TCGA | 1 cut(s) | 294 |
| TasI | AATT | 2 cut(s) | 54, 88 |
| TauI | GCSGC | 1 cut(s) | 191 |
| Tru1I | TTAA | 2 cut(s) | 267, 312 |
| Tru9I | TTAA | 2 cut(s) | 267, 312 |
| TscAI | CASTG | 1 cut(s) | 171 |
| TseI | GCWGC | 2 cut(s) | 18, 175 |
| TspDTI | ATGAA | 2 cut(s) | 17, 303 |
| TspGWI | ACGGA | 1 cut(s) | 141 |
| TspRI | CASTG | 1 cut(s) | 171 |
| Vha464I | CTTAAG | 1 cut(s) | 266 |
| XhoI | CTCGAG | 1 cut(s) | 293 |