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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 146 |
| AcsI | RAATTY | 1 cut(s) | 9 |
| AfaI | GTAC | 2 cut(s) | 52, 103 |
| AgsI | TTSAA | 3 cut(s) | 256, 266, 290 |
| AjnI | CCWGG | 1 cut(s) | 73 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 282 |
| AluBI | AGCT | 3 cut(s) | 211, 280, 286 |
| AluI | AGCT | 3 cut(s) | 211, 280, 286 |
| Alw26I | GTCTC | 1 cut(s) | 302 |
| AoxI | GGCC | 1 cut(s) | 153 |
| ApoI | RAATTY | 1 cut(s) | 9 |
| AsuC2I | CCSGG | 1 cut(s) | 302 |
| BccI | CCATC | 1 cut(s) | 83 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 227, 261 |
| BciT130I | CCWGG | 1 cut(s) | 75 |
| BcnI | CCSGG | 1 cut(s) | 302 |
| BcoDI | GTCTC | 1 cut(s) | 302 |
| Bme1390I | CCNGG | 2 cut(s) | 75, 302 |
| BmrFI | CCNGG | 2 cut(s) | 75, 302 |
| BpuEI | CTTGAG | 1 cut(s) | 302 |
| BpuMI | CCSGG | 1 cut(s) | 302 |
| BsaBI | GATNNNNATC | 1 cut(s) | 190 |
| BsaI | GGTCTC | 1 cut(s) | 302 |
| BsaJI | CCNNGG | 1 cut(s) | 74 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 282 |
| Bse8I | GATNNNNATC | 1 cut(s) | 190 |
| BseBI | CCWGG | 1 cut(s) | 75 |
| BseDI | CCNNGG | 1 cut(s) | 74 |
| BseJI | GATNNNNATC | 1 cut(s) | 190 |
| BshFI | GGCC | 1 cut(s) | 155 |
| BsiSI | CCGG | 1 cut(s) | 301 |
| BsmAI | GTCTC | 1 cut(s) | 302 |
| BsnI | GGCC | 1 cut(s) | 155 |
| Bso31I | GGTCTC | 1 cut(s) | 302 |
| Bsp143I | GATC | 1 cut(s) | 23 |
| BspANI | GGCC | 1 cut(s) | 155 |
| BspQI | GCTCTTC | 1 cut(s) | 228 |
| BspTNI | GGTCTC | 1 cut(s) | 302 |
| BssECI | CCNNGG | 1 cut(s) | 74 |
| BssMI | GATC | 1 cut(s) | 23 |
| Bst2UI | CCWGG | 1 cut(s) | 75 |
| Bst4CI | ACNGT | 1 cut(s) | 101 |
| Bst6I | CTCTTC | 1 cut(s) | 228 |
| BstKTI | GATC | 1 cut(s) | 26 |
| BstMAI | GTCTC | 1 cut(s) | 302 |
| BstMBI | GATC | 1 cut(s) | 23 |
| BstNI | CCWGG | 1 cut(s) | 75 |
| BstSCI | CCNGG | 2 cut(s) | 73, 300 |
| BsuRI | GGCC | 1 cut(s) | 155 |
| Csp6I | GTAC | 2 cut(s) | 51, 102 |
| CviAII | CATG | 1 cut(s) | 119 |
| CviJI | RGCY | 5 cut(s) | 109, 155, 211, 280, 286 |
| CviKI_1 | RGCY | 5 cut(s) | 109, 155, 211, 280, 286 |
| CviQI | GTAC | 2 cut(s) | 51, 102 |
| DpnI | GATC | 1 cut(s) | 25 |
| DpnII | GATC | 1 cut(s) | 23 |
| Eam1104I | CTCTTC | 1 cut(s) | 228 |
| EarI | CTCTTC | 1 cut(s) | 228 |
| Eco31I | GGTCTC | 1 cut(s) | 302 |
| EcoRII | CCWGG | 1 cut(s) | 73 |
| FaeI | CATG | 1 cut(s) | 122 |
| FaiI | YATR | 4 cut(s) | 120, 161, 214, 243 |
| FatI | CATG | 1 cut(s) | 118 |
| FblI | GTMKAC | 1 cut(s) | 146 |
| HaeIII | GGCC | 1 cut(s) | 155 |
| HapII | CCGG | 1 cut(s) | 301 |
| Hin1II | CATG | 1 cut(s) | 122 |
| HinfI | GANTC | 1 cut(s) | 115 |
| HpaII | CCGG | 1 cut(s) | 301 |
| Hpy166II | GTNNAC | 1 cut(s) | 147 |
| Hpy188I | TCNGA | 1 cut(s) | 85 |
| Hpy8I | GTNNAC | 1 cut(s) | 147 |
| HpyCH4III | ACNGT | 1 cut(s) | 101 |
| HpyCH4V | TGCA | 1 cut(s) | 21 |
| Hsp92II | CATG | 1 cut(s) | 122 |
| Kzo9I | GATC | 1 cut(s) | 23 |
| LguI | GCTCTTC | 1 cut(s) | 228 |
| LpnPI | CCDG | 3 cut(s) | 60, 87, 123 |
| MalI | GATC | 1 cut(s) | 25 |
| MboI | GATC | 1 cut(s) | 23 |
| MboII | GAAGA | 4 cut(s) | 235, 245, 278, 281 |
| MluCI | AATT | 3 cut(s) | 9, 54, 65 |
| MlyI | GAGTC | 1 cut(s) | 124 |
| MmeI | TCCRAC | 2 cut(s) | 153, 163 |
| MnlI | CCTC | 2 cut(s) | 136, 212 |
| MseI | TTAA | 1 cut(s) | 207 |
| MspI | CCGG | 1 cut(s) | 301 |
| MspR9I | CCNGG | 2 cut(s) | 75, 302 |
| MvaI | CCWGG | 1 cut(s) | 75 |
| NciI | CCSGG | 1 cut(s) | 302 |
| NdeII | GATC | 1 cut(s) | 23 |
| NlaIII | CATG | 1 cut(s) | 122 |
| PciSI | GCTCTTC | 1 cut(s) | 228 |
| PfoI | TCCNGGA | 1 cut(s) | 300 |
| PleI | GAGTC | 1 cut(s) | 123 |
| PpsI | GAGTC | 1 cut(s) | 123 |
| Psp6I | CCWGG | 1 cut(s) | 73 |
| PspGI | CCWGG | 1 cut(s) | 73 |
| RsaI | GTAC | 2 cut(s) | 52, 103 |
| RsaNI | GTAC | 2 cut(s) | 51, 102 |
| SapI | GCTCTTC | 1 cut(s) | 228 |
| SaqAI | TTAA | 1 cut(s) | 207 |
| Sau3AI | GATC | 1 cut(s) | 23 |
| SchI | GAGTC | 1 cut(s) | 124 |
| ScrFI | CCNGG | 2 cut(s) | 75, 302 |
| SetI | ASST | 6 cut(s) | 19, 128, 166, 213, 282, 288 |
| SmlI | CTYRAG | 1 cut(s) | 281 |
| SmoI | CTYRAG | 1 cut(s) | 281 |
| Sse9I | AATT | 3 cut(s) | 9, 54, 65 |
| StyD4I | CCNGG | 2 cut(s) | 73, 300 |
| TaaI | ACNGT | 1 cut(s) | 101 |
| TasI | AATT | 3 cut(s) | 9, 54, 65 |
| TatI | WGTACW | 1 cut(s) | 101 |
| Tru1I | TTAA | 1 cut(s) | 207 |
| Tru9I | TTAA | 1 cut(s) | 207 |
| TspDTI | ATGAA | 1 cut(s) | 58 |
| XapI | RAATTY | 1 cut(s) | 9 |
| XmiI | GTMKAC | 1 cut(s) | 146 |