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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 155 |
| AcsI | RAATTY | 1 cut(s) | 9 |
| AgsI | TTSAA | 4 cut(s) | 153, 214, 262, 272 |
| AjnI | CCWGG | 1 cut(s) | 73 |
| AluBI | AGCT | 4 cut(s) | 50, 217, 286, 292 |
| AluI | AGCT | 4 cut(s) | 50, 217, 286, 292 |
| Alw21I | GWGCWC | 1 cut(s) | 294 |
| AoxI | GGCC | 1 cut(s) | 76 |
| ApoI | RAATTY | 1 cut(s) | 9 |
| AsuC2I | CCSGG | 1 cut(s) | 253 |
| BanII | GRGCYC | 1 cut(s) | 294 |
| BbsI | GAAGAC | 1 cut(s) | 296 |
| Bbv12I | GWGCWC | 1 cut(s) | 294 |
| BccI | CCATC | 1 cut(s) | 101 |
| BciT130I | CCWGG | 1 cut(s) | 75 |
| BcnI | CCSGG | 1 cut(s) | 253 |
| BfaI | CTAG | 1 cut(s) | 96 |
| Bme1390I | CCNGG | 2 cut(s) | 75, 253 |
| BmrFI | CCNGG | 2 cut(s) | 75, 253 |
| BpiI | GAAGAC | 1 cut(s) | 296 |
| BpuEI | CTTGAG | 1 cut(s) | 308 |
| BpuMI | CCSGG | 1 cut(s) | 253 |
| BsaJI | CCNNGG | 1 cut(s) | 172 |
| Bse1I | ACTGG | 1 cut(s) | 151 |
| BseBI | CCWGG | 1 cut(s) | 75 |
| BseDI | CCNNGG | 1 cut(s) | 172 |
| BseGI | GGATG | 1 cut(s) | 283 |
| BseMII | CTCAG | 1 cut(s) | 111 |
| BseNI | ACTGG | 1 cut(s) | 151 |
| BshFI | GGCC | 1 cut(s) | 78 |
| BsiHKAI | GWGCWC | 1 cut(s) | 294 |
| BsiSI | CCGG | 1 cut(s) | 253 |
| BsnI | GGCC | 1 cut(s) | 78 |
| Bsp1286I | GDGCHC | 1 cut(s) | 294 |
| Bsp143I | GATC | 1 cut(s) | 23 |
| BspANI | GGCC | 1 cut(s) | 78 |
| BspCNI | CTCAG | 1 cut(s) | 112 |
| BsrI | ACTGG | 1 cut(s) | 151 |
| BssECI | CCNNGG | 1 cut(s) | 172 |
| BssMI | GATC | 1 cut(s) | 23 |
| BssT1I | CCWWGG | 1 cut(s) | 172 |
| Bst2UI | CCWGG | 1 cut(s) | 75 |
| Bst4CI | ACNGT | 1 cut(s) | 246 |
| Bst6I | CTCTTC | 1 cut(s) | 267 |
| BstDEI | CTNAG | 1 cut(s) | 120 |
| BstF5I | GGATG | 1 cut(s) | 283 |
| BstKTI | GATC | 1 cut(s) | 26 |
| BstMBI | GATC | 1 cut(s) | 23 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 34 |
| BstNI | CCWGG | 1 cut(s) | 75 |
| BstSCI | CCNGG | 2 cut(s) | 73, 251 |
| BstV2I | GAAGAC | 1 cut(s) | 296 |
| BsuRI | GGCC | 1 cut(s) | 78 |
| BtsCI | GGATG | 1 cut(s) | 283 |
| BtsIMutI | CAGTG | 1 cut(s) | 144 |
| CviJI | RGCY | 7 cut(s) | 50, 78, 141, 217, 256, 286, 292 |
| CviKI_1 | RGCY | 7 cut(s) | 50, 78, 141, 217, 256, 286, 292 |
| DdeI | CTNAG | 1 cut(s) | 120 |
| DpnI | GATC | 1 cut(s) | 25 |
| DpnII | GATC | 1 cut(s) | 23 |
| Eam1104I | CTCTTC | 1 cut(s) | 267 |
| EarI | CTCTTC | 1 cut(s) | 267 |
| Ecl136II | GAGCTC | 1 cut(s) | 292 |
| Eco130I | CCWWGG | 1 cut(s) | 172 |
| Eco147I | AGGCCT | 1 cut(s) | 78 |
| Eco24I | GRGCYC | 1 cut(s) | 294 |
| Eco53kI | GAGCTC | 1 cut(s) | 292 |
| EcoICRI | GAGCTC | 1 cut(s) | 292 |
| EcoRII | CCWGG | 1 cut(s) | 73 |
| EcoT14I | CCWWGG | 1 cut(s) | 172 |
| EcoT38I | GRGCYC | 1 cut(s) | 294 |
| ErhI | CCWWGG | 1 cut(s) | 172 |
| FaiI | YATR | 3 cut(s) | 167, 220, 249 |
| FokI | GGATG | 1 cut(s) | 290 |
| FriOI | GRGCYC | 1 cut(s) | 294 |
| FspBI | CTAG | 1 cut(s) | 96 |
| HaeIII | GGCC | 1 cut(s) | 78 |
| HapII | CCGG | 1 cut(s) | 253 |
| HinfI | GANTC | 2 cut(s) | 89, 124 |
| HpaII | CCGG | 1 cut(s) | 253 |
| Hpy188I | TCNGA | 2 cut(s) | 85, 121 |
| Hpy188III | TCNNGA | 1 cut(s) | 308 |
| HpyCH4III | ACNGT | 1 cut(s) | 246 |
| HpyCH4IV | ACGT | 1 cut(s) | 155 |
| HpyCH4V | TGCA | 1 cut(s) | 21 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 34 |
| HpyF3I | CTNAG | 1 cut(s) | 120 |
| HpySE526I | ACGT | 1 cut(s) | 155 |
| Kzo9I | GATC | 1 cut(s) | 23 |
| LpnPI | CCDG | 5 cut(s) | 60, 87, 132, 151, 266 |
| MaeI | CTAG | 1 cut(s) | 96 |
| MaeII | ACGT | 1 cut(s) | 155 |
| MalI | GATC | 1 cut(s) | 25 |
| MboI | GATC | 1 cut(s) | 23 |
| MboII | GAAGA | 4 cut(s) | 241, 251, 284, 296 |
| MhlI | GDGCHC | 1 cut(s) | 294 |
| MluCI | AATT | 2 cut(s) | 9, 65 |
| MlyI | GAGTC | 1 cut(s) | 133 |
| MnlI | CCTC | 1 cut(s) | 268 |
| MspI | CCGG | 1 cut(s) | 253 |
| MspR9I | CCNGG | 2 cut(s) | 75, 253 |
| MvaI | CCWGG | 1 cut(s) | 75 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 34 |
| NciI | CCSGG | 1 cut(s) | 253 |
| NdeII | GATC | 1 cut(s) | 23 |
| PceI | AGGCCT | 1 cut(s) | 78 |
| PfeI | GAWTC | 1 cut(s) | 89 |
| PleI | GAGTC | 1 cut(s) | 132 |
| PpsI | GAGTC | 1 cut(s) | 132 |
| Psp124BI | GAGCTC | 1 cut(s) | 294 |
| Psp1406I | AACGTT | 1 cut(s) | 155 |
| Psp6I | CCWGG | 1 cut(s) | 73 |
| PspGI | CCWGG | 1 cut(s) | 73 |
| SacI | GAGCTC | 1 cut(s) | 294 |
| Sau3AI | GATC | 1 cut(s) | 23 |
| SchI | GAGTC | 1 cut(s) | 133 |
| ScrFI | CCNGG | 2 cut(s) | 75, 253 |
| SduI | GDGCHC | 1 cut(s) | 294 |
| SetI | ASST | 5 cut(s) | 52, 158, 219, 288, 294 |
| SmlI | CTYRAG | 1 cut(s) | 287 |
| SmoI | CTYRAG | 1 cut(s) | 287 |
| Sse9I | AATT | 2 cut(s) | 9, 65 |
| SseBI | AGGCCT | 1 cut(s) | 78 |
| SspMI | CTAG | 1 cut(s) | 96 |
| SstI | GAGCTC | 1 cut(s) | 294 |
| StuI | AGGCCT | 1 cut(s) | 78 |
| StyD4I | CCNGG | 2 cut(s) | 73, 251 |
| StyI | CCWWGG | 1 cut(s) | 172 |
| TaaI | ACNGT | 1 cut(s) | 246 |
| TaiI | ACGT | 1 cut(s) | 158 |
| TaqI | TCGA | 1 cut(s) | 294 |
| TaqII | GACCGA | 1 cut(s) | 290 |
| TasI | AATT | 2 cut(s) | 9, 65 |
| TfiI | GAWTC | 1 cut(s) | 89 |
| TscAI | CASTG | 1 cut(s) | 151 |
| TspDTI | ATGAA | 2 cut(s) | 42, 81 |
| TspRI | CASTG | 1 cut(s) | 151 |
| XapI | RAATTY | 1 cut(s) | 9 |
| XspI | CTAG | 1 cut(s) | 96 |