Rorug07G0258100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001184
Physical Location & Seq
Reverse (-)
24203390 .. 24207901
4512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0258100.1

Sequence Viewer

Length: 1224 bp
ATGCAGGTCTCAGGTGTCATCGGCGACGTTTCACTGGTAATTCCCTCAGGCGGAGGTCTCCGGTCGCCGGAATTCCGACACTTCCACTCCCACACCTACACCCATTCACACTCGCTCTCTGATTCCAAGTCATTCATTTCCGGTGGATTTTTCAGAAGTTGTCTCCCAAAGCCTGGAAACGGACTGTATAAGTACCCAGCCAGAGGCTTGATTGTGAGAGCATCGGAGGATTCAAGTGATAATTTGGTGCCGGTTGCTCCTCTTCAGTTTGAATCCCCTGCTGGCCAGCTTTTGGGCCAAATACTACAAACACATCCACATCTACTACCTGCAGCTGTTGATCAGCAGCTCGAGAAACTTCAAACTGATAGAGATGCCGAAAAAGGAGAATCTCCAACATCTAAAGGTCCCCTTTACAAGAGAATAGCTGAGGTCAAAGAGAAAGAAGGGCGAGCGGCACTGCAAGAGATAATATACTGCTTAATAGTGCAAAAGTTTGTGGAGAATGAAATCTCAATGATCCCCAAGATATCAGCAACCTCGGATCCTACCGGCAGAGTGGATTTTTGGCCGAACCAAGAACAAAAACTAGAATCTGTTCATTCTTCCGAAGCATTTGAGATGATACAGAGCCACTTGTCTCTTGTGCTTGGAGAACGACTTGTCGGCGTGGGTCCCCTTTCTTCCTTGGTTGAGATTAGCAAAATAAAACTTGGAAAGCTGTATGCTGCTTCAATAATGTATGGATATTTCCTCAAAAGAGTTGATCAGCGGTTTCAGCTTGAGAGAACCATGAACATCCTTCCAAAACGCGTTACTCAGGATCCGGATCCGGCACCAGCAAACCAGCTATGGGATCCTGATTCCTTGTTCAGGATACCTCCTGATGGTGGTGATGAAGCAGGCTTCATGGATAATGGGGAAAGCAAGTCTTATCGACTGAGATCCTATGTGATGTACTTGGATGCAGAGACTCTTCAGAGCTATGCTACTATAAGGTCCAAGGAAGCTATTTCTTTGATTGAAAACCAGACGCAAGCCCTGTTTGGGAAACCTGACATCAGGATTGCTGGGGATGGTTCAATTGATACATCCAACGATGAAGTGATTTCACTTACTTTCACTGGATTGACAATGCTAGTCTTGGAAGCTGTTGCATTTGGATCATTCCTGTGGGATGCTGAAACCTATGTTGAATCCAAATATAACTTTGTCCAAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

45.1

Weight (kDa)

5.39

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF760 PF05542 138 - 265 2.2e-20 Protein of unknown function (DUF760)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 337
AccB1I GGYRCC 2 cut(s) 247, 835
AccB7I CCANNNNNTGG 2 cut(s) 173, 292
AccBSI CCGCTC 1 cut(s) 455
AccII CGCG 1 cut(s) 813
AccIII TCCGGA 1 cut(s) 826
AciI CCGC 3 cut(s) 51, 455, 772
AcoI YGGCCR 2 cut(s) 283, 569
AcsI RAATTY 1 cut(s) 71
AcuI CTGAAG 2 cut(s) 248, 962
AfaI GTAC 2 cut(s) 194, 959
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 7 cut(s) 234, 272, 362, 735, 1025, 1083, 1196
AjnI CCWGG 1 cut(s) 172
Alw26I GTCTC 5 cut(s) 13, 62, 167, 645, 965
Ama87I CYCGRG 1 cut(s) 350
Aor13HI TCCGGA 1 cut(s) 826
AoxI GGCC 3 cut(s) 283, 295, 569
ApeKI GCWGC 3 cut(s) 332, 346, 728
ApoI RAATTY 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 597
AspS9I GGNCC 4 cut(s) 295, 407, 674, 999
AsuHPI GGTGA 1 cut(s) 905
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 3 cut(s) 407, 674, 999
AxyI CCTNAGG 1 cut(s) 46
BalI TGGCCA 1 cut(s) 285
BamHI GGATCC 4 cut(s) 544, 823, 829, 856
BanI GGYRCC 2 cut(s) 247, 835
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 344, 358, 715
BccI CCATC 2 cut(s) 881, 1070
BciT130I CCWGG 1 cut(s) 174
BciVI GTATCC 1 cut(s) 870
BclI TGATCA 2 cut(s) 340, 766
BcoDI GTCTC 5 cut(s) 13, 62, 167, 645, 965
BfaI CTAG 3 cut(s) 590, 1139, 1222
BfmI CTRYAG 1 cut(s) 330
BfuAI ACCTGC 1 cut(s) 337
BfuI GTATCC 1 cut(s) 870
BisI GCNGC 4 cut(s) 333, 347, 456, 729
BlsI GCNGC 4 cut(s) 334, 348, 457, 730
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 3 cut(s) 407, 674, 999
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 4 cut(s) 295, 407, 674, 999
BmiI GGNNCC 9 cut(s) 249, 409, 546, 675, 676, 825, 831, 837, 858
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 4 cut(s) 230, 364, 955, 1168
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 803
BsaBI GATNNNNATC 1 cut(s) 828
BsaI GGTCTC 2 cut(s) 13, 62
BsaJI CCNNGG 3 cut(s) 540, 687, 1002
BsaWI WCCGGW 3 cut(s) 60, 140, 826
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bse118I RCCGGY 2 cut(s) 250, 551
Bse1I ACTGG 2 cut(s) 39, 1129
Bse21I CCTNAGG 1 cut(s) 46
Bse8I GATNNNNATC 1 cut(s) 828
BseAI TCCGGA 1 cut(s) 826
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 540, 687, 1002
BseGI GGATG 6 cut(s) 313, 798, 970, 1081, 1091, 1183
BseJI GATNNNNATC 1 cut(s) 828
BseMII CTCAG 5 cut(s) 24, 60, 420, 833, 932
BseNI ACTGG 2 cut(s) 39, 1129
BseRI GAGGAG 1 cut(s) 249
BseXI GCAGC 3 cut(s) 344, 358, 715
BseYI CCCAGC 2 cut(s) 196, 1070
Bsh1236I CGCG 1 cut(s) 813
Bsh1285I CGRYCG 1 cut(s) 65
BshFI GGCC 3 cut(s) 285, 297, 571
BshNI GGYRCC 2 cut(s) 247, 835
BsiEI CGRYCG 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 7 cut(s) 61, 68, 141, 251, 552, 827, 833
BslFI GGGAC 2 cut(s) 393, 660
BsmAI GTCTC 5 cut(s) 13, 62, 167, 645, 965
BsmFI GGGAC 2 cut(s) 393, 660
BsnI GGCC 3 cut(s) 285, 297, 571
Bso31I GGTCTC 2 cut(s) 13, 62
BsoBI CYCGRG 1 cut(s) 350
Bsp13I TCCGGA 1 cut(s) 826
Bsp143I GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
BspACI CCGC 3 cut(s) 51, 455, 772
BspANI GGCC 3 cut(s) 285, 297, 571
BspCNI CTCAG 5 cut(s) 23, 59, 421, 832, 933
BspEI TCCGGA 1 cut(s) 826
BspFNI CGCG 1 cut(s) 813
BspLI GGNNCC 9 cut(s) 249, 409, 546, 675, 676, 825, 831, 837, 858
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 337
BspT107I GGYRCC 2 cut(s) 247, 835
BspTNI GGTCTC 2 cut(s) 13, 62
BsrBI CCGCTC 1 cut(s) 455
BsrFI RCCGGY 2 cut(s) 250, 551
BsrI ACTGG 2 cut(s) 39, 1129
BssAI RCCGGY 2 cut(s) 250, 551
BssECI CCNNGG 3 cut(s) 540, 687, 1002
BssMI GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
BssT1I CCWWGG 2 cut(s) 687, 1002
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 1 cut(s) 186
Bst6I CTCTTC 2 cut(s) 267, 981
BstC8I GCNNGC 5 cut(s) 283, 287, 453, 904, 1038
BstDEI CTNAG 5 cut(s) 10, 46, 429, 819, 941
BstENI CCTNNNNNAGG 1 cut(s) 871
BstF5I GGATG 6 cut(s) 313, 798, 970, 1081, 1091, 1183
BstFNI CGCG 1 cut(s) 813
BstKTI GATC 9 cut(s) 343, 522, 547, 769, 826, 832, 859, 947, 1166
BstMAI GTCTC 5 cut(s) 13, 62, 167, 645, 965
BstMBI GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
BstMCI CGRYCG 1 cut(s) 65
BstMWI GCNNNNNNNGC 1 cut(s) 778
BstNI CCWGG 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 1 cut(s) 813
BstV1I GCAGC 3 cut(s) 344, 358, 715
BstX2I RGATCY 5 cut(s) 544, 823, 829, 856, 944
BstYI RGATCY 5 cut(s) 544, 823, 829, 856, 944
Bsu36I CCTNAGG 1 cut(s) 46
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 3 cut(s) 285, 297, 571
BtsCI GGATG 6 cut(s) 313, 798, 970, 1081, 1091, 1183
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 3 cut(s) 32, 458, 1122
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 5 cut(s) 283, 287, 453, 904, 1038
Cfr10I RCCGGY 2 cut(s) 250, 551
Cfr13I GGNCC 4 cut(s) 295, 407, 674, 999
CseI GACGC 1 cut(s) 1042
Csp6I GTAC 2 cut(s) 193, 958
CviAII CATG 2 cut(s) 793, 910
CviQI GTAC 2 cut(s) 193, 958
DdeI CTNAG 5 cut(s) 10, 46, 429, 819, 941
DpnI GATC 9 cut(s) 342, 521, 546, 768, 825, 831, 858, 946, 1165
DpnII GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
EaeI YGGCCR 2 cut(s) 283, 569
Eam1104I CTCTTC 2 cut(s) 267, 981
EarI CTCTTC 2 cut(s) 267, 981
EciI GGCGGA 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 687, 1002
Eco31I GGTCTC 2 cut(s) 13, 62
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 3 cut(s) 407, 674, 999
Eco57I CTGAAG 2 cut(s) 248, 962
Eco81I CCTNAGG 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 871
EcoO109I RGGNCCY 2 cut(s) 407, 674
EcoRI GAATTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 172
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 2 cut(s) 687, 1002
ErhI CCWWGG 2 cut(s) 687, 1002
FaeI CATG 2 cut(s) 796, 913
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 916, 948
FaqI GGGAC 2 cut(s) 393, 660
FatI CATG 2 cut(s) 792, 909
FbaI TGATCA 2 cut(s) 340, 766
Fnu4HI GCNGC 4 cut(s) 333, 347, 456, 729
FokI GGATG 6 cut(s) 300, 785, 977, 1078, 1088, 1190
Fsp4HI GCNGC 4 cut(s) 333, 347, 456, 729
FspBI CTAG 3 cut(s) 590, 1139, 1222
GluI GCNGC 4 cut(s) 333, 347, 456, 729
GsaI CCCAGC 2 cut(s) 200, 1074
HaeIII GGCC 3 cut(s) 285, 297, 571
HapII CCGG 7 cut(s) 61, 68, 141, 251, 552, 827, 833
HgaI GACGC 1 cut(s) 1042
Hin1II CATG 2 cut(s) 796, 913
HinfI GANTC 8 cut(s) 122, 230, 272, 389, 593, 863, 973, 1196
HpaII CCGG 7 cut(s) 61, 68, 141, 251, 552, 827, 833
HphI GGTGA 1 cut(s) 905
Hpy188I TCNGA 7 cut(s) 77, 121, 155, 226, 544, 610, 981
Hpy188III TCNNGA 7 cut(s) 352, 821, 827, 860, 874, 884, 1063
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 440, 812
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 6 cut(s) 4, 332, 463, 490, 968, 1157
HpyF10VI GCNNNNNNNGC 1 cut(s) 778
HpyF3I CTNAG 5 cut(s) 10, 46, 429, 819, 941
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 2 cut(s) 796, 913
KflI GGGWCCC 1 cut(s) 674
Kpn2I TCCGGA 1 cut(s) 826
Ksp22I TGATCA 2 cut(s) 340, 766
Kzo9I GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 3 cut(s) 344, 358, 715
LweI GCATC 4 cut(s) 230, 364, 955, 1168
MaeI CTAG 3 cut(s) 590, 1139, 1222
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 814
MalI GATC 9 cut(s) 342, 521, 546, 768, 825, 831, 858, 946, 1165
MbiI CCGCTC 1 cut(s) 455
MboI GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
MboII GAAGA 4 cut(s) 254, 597, 675, 968
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 5 cut(s) 544, 823, 829, 856, 944
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 4 cut(s) 39, 71, 241, 1083
MluI ACGCGT 1 cut(s) 811
MluNI TGGCCA 1 cut(s) 285
MlyI GAGTC 1 cut(s) 967
MmeI TCCRAC 3 cut(s) 100, 419, 1119
MnlI CCTC 9 cut(s) 47, 55, 197, 220, 270, 424, 550, 764, 891
Mox20I TGGCCA 1 cut(s) 285
MroI TCCGGA 1 cut(s) 826
MroXI GAANNNNTTC 1 cut(s) 597
MscI TGGCCA 1 cut(s) 285
MseI TTAA 1 cut(s) 482
MslI CAYNNNNRTG 1 cut(s) 1171
Msp20I TGGCCA 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 335, 772
MspI CCGG 7 cut(s) 61, 68, 141, 251, 552, 827, 833
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 1 cut(s) 1083
MvaI CCWGG 1 cut(s) 174
MvnI CGCG 1 cut(s) 813
MwoI GCNNNNNNNGC 1 cut(s) 778
NdeII GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
NlaIII CATG 2 cut(s) 796, 913
NlaIV GGNNCC 9 cut(s) 249, 409, 546, 675, 676, 825, 831, 837, 858
PaeR7I CTCGAG 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 597
PfeI GAWTC 7 cut(s) 122, 230, 272, 389, 593, 863, 1196
PflMI CCANNNNNTGG 2 cut(s) 173, 292
PkrI GCNGC 4 cut(s) 334, 348, 457, 730
PleI GAGTC 1 cut(s) 967
PpsI GAGTC 1 cut(s) 967
PpuMI RGGWCCY 2 cut(s) 407, 674
Psp5II RGGWCCY 2 cut(s) 407, 674
Psp6I CCWGG 1 cut(s) 172
PspFI CCCAGC 2 cut(s) 196, 1070
PspGI CCWGG 1 cut(s) 172
PspN4I GGNNCC 9 cut(s) 249, 409, 546, 675, 676, 825, 831, 837, 858
PspPI GGNCC 4 cut(s) 295, 407, 674, 999
PspPPI RGGWCCY 2 cut(s) 407, 674
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 5 cut(s) 544, 823, 829, 856, 944
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 2 cut(s) 194, 959
RsaNI GTAC 2 cut(s) 193, 958
RseI CAYNNNNRTG 1 cut(s) 1171
SaqAI TTAA 1 cut(s) 482
SatI GCNGC 4 cut(s) 333, 347, 456, 729
Sau3AI GATC 9 cut(s) 340, 519, 544, 766, 823, 829, 856, 944, 1163
Sau96I GGNCC 4 cut(s) 295, 407, 674, 999
SchI GAGTC 1 cut(s) 967
ScrFI CCNGG 1 cut(s) 174
SfaNI GCATC 4 cut(s) 230, 364, 955, 1168
SfcI CTRYAG 1 cut(s) 330
Sfr274I CTCGAG 1 cut(s) 350
SinI GGWCC 3 cut(s) 407, 674, 999
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 1171
SmlI CTYRAG 2 cut(s) 350, 782
SmoI CTYRAG 2 cut(s) 350, 782
Sse9I AATT 4 cut(s) 39, 71, 241, 1083
SsiI CCGC 3 cut(s) 51, 455, 772
SspMI CTAG 3 cut(s) 590, 1139, 1222
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 2 cut(s) 687, 1002
TaaI ACNGT 1 cut(s) 186
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 351, 937
TasI AATT 4 cut(s) 39, 71, 241, 1083
TatI WGTACW 1 cut(s) 957
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 7 cut(s) 122, 230, 272, 389, 593, 863, 1196
Tru1I TTAA 1 cut(s) 482
Tru9I TTAA 1 cut(s) 482
TscAI CASTG 3 cut(s) 39, 465, 1129
TseI GCWGC 3 cut(s) 332, 346, 728
TspDTI ATGAA 7 cut(s) 124, 522, 590, 809, 898, 912, 1116
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 3 cut(s) 39, 465, 1129
Van91I CCANNNNNTGG 2 cut(s) 173, 292
VpaK11BI GGWCC 3 cut(s) 407, 674, 999
XagI CCTNNNNNAGG 1 cut(s) 871
XapI RAATTY 1 cut(s) 71
XhoI CTCGAG 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 3 cut(s) 590, 1139, 1222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.