Rh5DG271600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001184
Physical Location & Seq
Forward (+)
33034979 .. 33035278
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG271600.1

Sequence Viewer

Length: 300 bp
ATGAATGGATCAGGAATCGGCAACCGATGCGTGCAATTCCTACTCCTCCTCCTCCTTTTCACAATCCCGTTGTTGTTTGATTCAGTTCGGGCAAAGGACCACGGAAGCAACAAAGTCGTTGGTTCTTCGGTAGCGCTGAAGGTAGTTATTGTGTTGCTGGGCGTGGCGGTGGTGGTTGGGTTCGGTGTGTTTCTGTTCAGGATATGGCAGAGGAAGAAGAGAGAGGAGCAGCACGCCAGGCTTCTCAAGTTGTTTGAAGACGACGATGAGCTCAAGGTTGAACTTGGAATTAGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

11.11

Weight (kDa)

9.36

Isoelectric Point (pI)

12.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 167
AclWI GGATC 1 cut(s) 16
AcuI CTGAAG 1 cut(s) 158
AfeI AGCGCT 1 cut(s) 135
AgsI TTSAA 2 cut(s) 257, 281
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 1 cut(s) 271
AluI AGCT 1 cut(s) 271
Alw21I GWGCWC 1 cut(s) 273
AlwI GGATC 1 cut(s) 16
Aor51HI AGCGCT 1 cut(s) 135
ApeKI GCWGC 1 cut(s) 229
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 1 cut(s) 97
AvaII GGWCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BciT130I CCWGG 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 137
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
Bme1390I CCNGG 1 cut(s) 238
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 97
BmrFI CCNGG 1 cut(s) 238
BmsI GCATC 1 cut(s) 17
BpiI GAAGAC 1 cut(s) 264
BpuEI CTTGAG 2 cut(s) 230, 257
BsaJI CCNNGG 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
BseBI CCWGG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 100
BseRI GAGGAG 4 cut(s) 35, 38, 41, 239
BseXI GCAGC 1 cut(s) 241
BseYI CCCAGC 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 1 cut(s) 8
BspACI CCGC 1 cut(s) 167
BspPI GGATC 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 1 cut(s) 8
Bst2UI CCWGG 1 cut(s) 238
Bst6I CTCTTC 1 cut(s) 212
BstAPI GCANNNNNTGC 1 cut(s) 27
BstC8I GCNNGC 2 cut(s) 32, 234
BstDSI CCRYGG 1 cut(s) 100
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 1 cut(s) 11
BstMBI GATC 1 cut(s) 8
BstMWI GCNNNNNNNGC 2 cut(s) 27, 238
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 100
Cac8I GCNNGC 2 cut(s) 32, 234
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 1 cut(s) 97
CviJI RGCY 2 cut(s) 241, 271
CviKI_1 RGCY 2 cut(s) 241, 271
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 271
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 97
Eco47III AGCGCT 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 273
FaiI YATR 1 cut(s) 205
Fnu4HI GCNGC 1 cut(s) 230
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 1 cut(s) 230
GlaI GCGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 230
GsaI CCCAGC 1 cut(s) 161
HaeII RGCGCY 1 cut(s) 137
HhaI GCGC 1 cut(s) 136
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HinfI GANTC 2 cut(s) 15, 80
Hpy188III TCNNGA 2 cut(s) 12, 199
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 1 cut(s) 133
HpyCH4V TGCA 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 238
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 4 cut(s) 143, 184, 223, 250
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 1 cut(s) 17
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MboII GAAGA 4 cut(s) 117, 226, 229, 269
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 2 cut(s) 35, 288
MnlI CCTC 5 cut(s) 56, 59, 62, 204, 217
MseI TTAA 1 cut(s) 298
MspR9I CCNGG 1 cut(s) 238
MvaI CCWGG 1 cut(s) 238
MwoI GCNNNNNNNGC 2 cut(s) 27, 238
NdeII GATC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 15, 80
PkrI GCNGC 1 cut(s) 231
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 1 cut(s) 236
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 236
PspPI GGNCC 1 cut(s) 97
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 298
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 1 cut(s) 8
Sau96I GGNCC 1 cut(s) 97
ScrFI CCNGG 1 cut(s) 238
SduI GDGCHC 1 cut(s) 273
SetI ASST 3 cut(s) 144, 273, 279
SfaNI GCATC 1 cut(s) 17
SinI GGWCC 1 cut(s) 97
SmlI CTYRAG 2 cut(s) 245, 272
SmoI CTYRAG 2 cut(s) 245, 272
Sse9I AATT 2 cut(s) 35, 288
SsiI CCGC 1 cut(s) 167
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 1 cut(s) 236
TasI AATT 2 cut(s) 35, 288
TfiI GAWTC 2 cut(s) 15, 80
Tru1I TTAA 1 cut(s) 298
Tru9I TTAA 1 cut(s) 298
TseI GCWGC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 117
VpaK11BI GGWCC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.