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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 199, 374 |
| AcsI | RAATTY | 1 cut(s) | 87 |
| AfaI | GTAC | 3 cut(s) | 9, 122, 128 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 311 |
| AgsI | TTSAA | 4 cut(s) | 85, 92, 327, 464 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 111 |
| AluBI | AGCT | 3 cut(s) | 323, 457, 490 |
| AluI | AGCT | 3 cut(s) | 323, 457, 490 |
| Alw26I | GTCTC | 1 cut(s) | 309 |
| Ama87I | CYCGRG | 2 cut(s) | 160, 479 |
| ApeKI | GCWGC | 1 cut(s) | 361 |
| ApoI | RAATTY | 1 cut(s) | 87 |
| AvaI | CYCGRG | 2 cut(s) | 160, 479 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 32 |
| BbsI | GAAGAC | 3 cut(s) | 388, 440, 471 |
| BbvI | GCAGC | 1 cut(s) | 348 |
| BccI | CCATC | 1 cut(s) | 70 |
| BcoDI | GTCTC | 1 cut(s) | 309 |
| BisI | GCNGC | 3 cut(s) | 199, 362, 375 |
| BlsI | GCNGC | 3 cut(s) | 200, 363, 376 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 111 |
| BmeT110I | CYCGRG | 2 cut(s) | 160, 479 |
| BmiI | GGNNCC | 2 cut(s) | 157, 197 |
| BpiI | GAAGAC | 3 cut(s) | 388, 440, 471 |
| BpuEI | CTTGAG | 2 cut(s) | 329, 437 |
| BsaJI | CCNNGG | 1 cut(s) | 208 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 286, 316 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 311 |
| Bse3DI | GCAATG | 1 cut(s) | 202 |
| BseDI | CCNNGG | 1 cut(s) | 208 |
| BseGI | GGATG | 2 cut(s) | 31, 62 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 311 |
| BseMI | GCAATG | 1 cut(s) | 202 |
| BseRI | GAGGAG | 2 cut(s) | 230, 251 |
| BseXI | GCAGC | 1 cut(s) | 348 |
| BseYI | CCCAGC | 1 cut(s) | 364 |
| BsiHKCI | CYCGRG | 2 cut(s) | 160, 479 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 311 |
| BsmAI | GTCTC | 1 cut(s) | 309 |
| BsmI | GAATGC | 1 cut(s) | 53 |
| BsoBI | CYCGRG | 2 cut(s) | 160, 479 |
| BspACI | CCGC | 2 cut(s) | 199, 374 |
| BspLI | GGNNCC | 2 cut(s) | 157, 197 |
| BspQI | GCTCTTC | 1 cut(s) | 426 |
| BsrDI | GCAATG | 1 cut(s) | 202 |
| BssECI | CCNNGG | 1 cut(s) | 208 |
| BssT1I | CCWWGG | 1 cut(s) | 208 |
| Bst6I | CTCTTC | 4 cut(s) | 240, 269, 419, 426 |
| BstC8I | GCNNGC | 1 cut(s) | 51 |
| BstF5I | GGATG | 2 cut(s) | 31, 62 |
| BstMAI | GTCTC | 1 cut(s) | 309 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 204, 347 |
| BstV1I | GCAGC | 1 cut(s) | 348 |
| BstV2I | GAAGAC | 3 cut(s) | 388, 440, 471 |
| BtsCI | GGATG | 2 cut(s) | 31, 62 |
| BtsI | GCAGTG | 1 cut(s) | 357 |
| BtsIMutI | CAGTG | 1 cut(s) | 357 |
| Cac8I | GCNNGC | 1 cut(s) | 51 |
| CseI | GACGC | 1 cut(s) | 382 |
| Csp6I | GTAC | 3 cut(s) | 8, 121, 127 |
| CviAII | CATG | 1 cut(s) | 181 |
| CviJI | RGCY | 9 cut(s) | 49, 198, 213, 258, 323, 377, 387, 457, 490 |
| CviKI_1 | RGCY | 9 cut(s) | 49, 198, 213, 258, 323, 377, 387, 457, 490 |
| CviQI | GTAC | 3 cut(s) | 8, 121, 127 |
| DriI | GACNNNNNGTC | 1 cut(s) | 111 |
| Eam1104I | CTCTTC | 4 cut(s) | 240, 269, 419, 426 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 111 |
| EarI | CTCTTC | 4 cut(s) | 240, 269, 419, 426 |
| Eco130I | CCWWGG | 1 cut(s) | 208 |
| Eco88I | CYCGRG | 2 cut(s) | 160, 479 |
| EcoT14I | CCWWGG | 1 cut(s) | 208 |
| ErhI | CCWWGG | 1 cut(s) | 208 |
| FaeI | CATG | 1 cut(s) | 184 |
| FaiI | YATR | 2 cut(s) | 182, 412 |
| FatI | CATG | 1 cut(s) | 180 |
| Fnu4HI | GCNGC | 3 cut(s) | 199, 362, 375 |
| FokI | GGATG | 2 cut(s) | 18, 49 |
| Fsp4HI | GCNGC | 3 cut(s) | 199, 362, 375 |
| GluI | GCNGC | 3 cut(s) | 199, 362, 375 |
| GsaI | CCCAGC | 1 cut(s) | 368 |
| HgaI | GACGC | 1 cut(s) | 382 |
| Hin1II | CATG | 1 cut(s) | 184 |
| HindIII | AAGCTT | 1 cut(s) | 321 |
| Hpy188I | TCNGA | 2 cut(s) | 36, 195 |
| Hpy188III | TCNNGA | 1 cut(s) | 406 |
| Hpy99I | CGWCG | 2 cut(s) | 386, 473 |
| HpyCH4V | TGCA | 4 cut(s) | 72, 79, 220, 226 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 204, 347 |
| Hsp92II | CATG | 1 cut(s) | 184 |
| LguI | GCTCTTC | 1 cut(s) | 426 |
| LmnI | GCTCC | 1 cut(s) | 195 |
| LpnPI | CCDG | 3 cut(s) | 35, 350, 391 |
| Lsp1109I | GCAGC | 1 cut(s) | 348 |
| MfeI | CAATTG | 1 cut(s) | 80 |
| MluCI | AATT | 5 cut(s) | 37, 74, 80, 87, 108 |
| MnlI | CCTC | 6 cut(s) | 243, 251, 269, 272, 301, 475 |
| MunI | CAATTG | 1 cut(s) | 80 |
| Mva1269I | GAATGC | 1 cut(s) | 53 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 204, 347 |
| NlaIII | CATG | 1 cut(s) | 184 |
| NlaIV | GGNNCC | 2 cut(s) | 157, 197 |
| PaeR7I | CTCGAG | 1 cut(s) | 479 |
| PciSI | GCTCTTC | 1 cut(s) | 426 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 390, 477 |
| PctI | GAATGC | 1 cut(s) | 53 |
| PkrI | GCNGC | 3 cut(s) | 200, 363, 376 |
| PspFI | CCCAGC | 1 cut(s) | 364 |
| PspN4I | GGNNCC | 2 cut(s) | 157, 197 |
| PspXI | VCTCGAGB | 1 cut(s) | 479 |
| RsaI | GTAC | 3 cut(s) | 9, 122, 128 |
| RsaNI | GTAC | 3 cut(s) | 8, 121, 127 |
| SapI | GCTCTTC | 1 cut(s) | 426 |
| SatI | GCNGC | 3 cut(s) | 199, 362, 375 |
| SetI | ASST | 5 cut(s) | 157, 325, 459, 486, 492 |
| Sfr274I | CTCGAG | 1 cut(s) | 479 |
| SlaI | CTCGAG | 1 cut(s) | 479 |
| SmlI | CTYRAG | 3 cut(s) | 344, 452, 479 |
| SmoI | CTYRAG | 3 cut(s) | 344, 452, 479 |
| Sse9I | AATT | 5 cut(s) | 37, 74, 80, 87, 108 |
| SsiI | CCGC | 2 cut(s) | 199, 374 |
| StyI | CCWWGG | 1 cut(s) | 208 |
| TaqI | TCGA | 1 cut(s) | 480 |
| TasI | AATT | 5 cut(s) | 37, 74, 80, 87, 108 |
| TatI | WGTACW | 1 cut(s) | 120 |
| TauI | GCSGC | 2 cut(s) | 201, 377 |
| TscAI | CASTG | 1 cut(s) | 357 |
| TseI | GCWGC | 1 cut(s) | 361 |
| TspDTI | ATGAA | 2 cut(s) | 197, 489 |
| TspGWI | ACGGA | 1 cut(s) | 327 |
| TspRI | CASTG | 1 cut(s) | 357 |
| XapI | RAATTY | 1 cut(s) | 87 |
| XhoI | CTCGAG | 1 cut(s) | 479 |