MD05G1254300.v1.1

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003915
Physical Location & Seq
Reverse (-)
38483912 .. 38485344
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1254300.v1.1.491

Sequence Viewer

Length: 531 bp
ATGTCTATGACGACTAAACCTCATTTCCAAGCAGAGCAAGATGAAGTAAAACAGGAGGGAAATAAGTACATCTTCAGACCACGGGGACTTAACATTGTATGGGGCAATGATGAACGCTACTGGAAGTTACCTAAGAAGCAATCTAAGGATGAACCTACTGAACCTGCGGAGCTGATACAGGTTTCTTGGTTGGAAATAACCGGATCATATAGTTTATCAACTGCAAAAAAATACGAAATTAGTTTCGACGTAGAACTGGCACCTGATGCATTTGGTTGGAGAGACATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAAGGGTAAGTACACTTGGACCAAAGTTAAAGTAGCAGCGCAGGATACAAAAGTCGGTAAATTCAAAATTCCTGATGACAACGGCCCGCCAATGAGAATTGAAGTTCCGTCCAATGCTCCGGATAGCACGGTTTATTTTGGTCTGTATGAAGTCTGGAGTGGAAAATGGAAGGGGGGCTTGAAAATTCATCAAGCATCTGTGAAAGAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

177

Amino Acids

20.1

Weight (kDa)

9.06

Isoelectric Point (pI)

25.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 21 - 171 2.6e-23 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 2 cut(s) 167, 404
AclWI GGATC 1 cut(s) 211
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 3 cut(s) 377, 384, 501
AcuI CTGAAG 1 cut(s) 58
AfaI GTAC 2 cut(s) 68, 329
AfiI CCNNNNNNNGG 1 cut(s) 312
AgsI TTSAA 3 cut(s) 382, 419, 499
AluBI AGCT 2 cut(s) 172, 293
AluI AGCT 2 cut(s) 172, 293
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 211
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 2 cut(s) 303, 400
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 3 cut(s) 377, 384, 501
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 2 cut(s) 336, 401
AvaII GGWCC 1 cut(s) 336
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
BalI TGGCCA 1 cut(s) 305
BanI GGYRCC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 365
BccI CCATC 1 cut(s) 295
BceAI ACGGC 1 cut(s) 415
BciVI GTATCC 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 172
BfuI GTATCC 1 cut(s) 355
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme18I GGWCC 1 cut(s) 336
BmgT120I GGNCC 2 cut(s) 336, 401
BmiI GGNNCC 1 cut(s) 261
BmsI GCATC 2 cut(s) 256, 521
BpmI CTGGAG 1 cut(s) 493
BsaJI CCNNGG 2 cut(s) 80, 306
BsaWI WCCGGW 2 cut(s) 200, 436
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse1I ACTGG 2 cut(s) 125, 261
Bse3DI GCAATG 1 cut(s) 112
BseAI TCCGGA 1 cut(s) 436
BseDI CCNNGG 2 cut(s) 80, 306
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 112
BseNI ACTGG 2 cut(s) 125, 261
BseXI GCAGC 1 cut(s) 365
BshFI GGCC 2 cut(s) 305, 402
BshNI GGYRCC 1 cut(s) 259
BsiSI CCGG 2 cut(s) 201, 437
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 2 cut(s) 305, 402
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 1 cut(s) 203
BspACI CCGC 2 cut(s) 167, 404
BspANI GGCC 2 cut(s) 305, 402
BspEI TCCGGA 1 cut(s) 436
BspLI GGNNCC 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 172
BspPI GGATC 1 cut(s) 211
BspT107I GGYRCC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 112
BsrI ACTGG 2 cut(s) 125, 261
BssECI CCNNGG 2 cut(s) 80, 306
BssMI GATC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 448
BstAPI GCANNNNNTGC 1 cut(s) 266
BstC8I GCNNGC 1 cut(s) 404
BstDEI CTNAG 2 cut(s) 132, 144
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 1 cut(s) 206
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 1 cut(s) 203
BstMWI GCNNNNNNNGC 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 365
BsuI GTATCC 1 cut(s) 355
BsuRI GGCC 2 cut(s) 305, 402
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 404
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 2 cut(s) 336, 401
Csp6I GTAC 2 cut(s) 67, 328
CviJI RGCY 5 cut(s) 172, 293, 305, 402, 495
CviKI_1 RGCY 5 cut(s) 172, 293, 305, 402, 495
CviQI GTAC 2 cut(s) 67, 328
DdeI CTNAG 2 cut(s) 132, 144
DpnI GATC 1 cut(s) 205
DpnII GATC 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 306
FaiI YATR 7 cut(s) 8, 100, 208, 210, 287, 465, 529
FalI AAGNNNNNCTT 4 cut(s) 56, 88, 479, 511
FaqI GGGAC 1 cut(s) 99
FauI CCCGC 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 1 cut(s) 161
Fsp4HI GCNGC 1 cut(s) 354
GlaI GCGC 1 cut(s) 357
GluI GCNGC 1 cut(s) 354
GsuI CTGGAG 1 cut(s) 493
HaeIII GGCC 2 cut(s) 305, 402
HapII CCGG 2 cut(s) 201, 437
HhaI GCGC 1 cut(s) 358
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 291
HpaII CCGG 2 cut(s) 201, 437
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 1 cut(s) 77
Hpy188III TCNNGA 4 cut(s) 298, 389, 437, 472
Hpy8I GTNNAC 1 cut(s) 330
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 481
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 2 cut(s) 224, 269
HpyF10VI GCNNNNNNNGC 1 cut(s) 266
HpyF3I CTNAG 2 cut(s) 132, 144
HpySE526I ACGT 1 cut(s) 249
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 436
Kzo9I GATC 1 cut(s) 203
LmnI GCTCC 2 cut(s) 169, 439
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 2 cut(s) 256, 521
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 126
MalI GATC 1 cut(s) 205
MboI GATC 1 cut(s) 203
MboII GAAGA 1 cut(s) 64
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 5 cut(s) 237, 377, 384, 414, 501
MluNI TGGCCA 1 cut(s) 305
MmeI TCCRAC 2 cut(s) 171, 257
MnlI CCTC 2 cut(s) 30, 49
Mox20I TGGCCA 1 cut(s) 305
Mph1103I ATGCAT 1 cut(s) 271
MroI TCCGGA 1 cut(s) 436
MscI TGGCCA 1 cut(s) 305
MseI TTAA 2 cut(s) 90, 345
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 2 cut(s) 201, 437
MwoI GCNNNNNNNGC 1 cut(s) 266
NdeII GATC 1 cut(s) 203
NlaIV GGNNCC 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 271
PkrI GCNGC 1 cut(s) 355
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 2 cut(s) 336, 401
RsaI GTAC 2 cut(s) 68, 329
RsaNI GTAC 2 cut(s) 67, 328
SaqAI TTAA 2 cut(s) 90, 345
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 1 cut(s) 203
Sau96I GGNCC 2 cut(s) 336, 401
SfaNI GCATC 2 cut(s) 256, 521
SinI GGWCC 1 cut(s) 336
Sse9I AATT 5 cut(s) 237, 377, 384, 414, 501
SsiI CCGC 2 cut(s) 167, 404
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 1 cut(s) 252
TaqI TCGA 1 cut(s) 246
TasI AATT 5 cut(s) 237, 377, 384, 414, 501
TatI WGTACW 2 cut(s) 66, 327
Tru1I TTAA 2 cut(s) 90, 345
Tru9I TTAA 2 cut(s) 90, 345
TseI GCWGC 1 cut(s) 353
TspDTI ATGAA 5 cut(s) 57, 126, 165, 480, 494
TspGWI ACGGA 1 cut(s) 414
VpaK11BI GGWCC 1 cut(s) 336
XapI RAATTY 3 cut(s) 377, 384, 501
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.