Rroxscaffold_1G00058260

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003915
Physical Location & Seq
Reverse (-)
80094554 .. 80096023
1470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058260.1

Sequence Viewer

Length: 513 bp
ATGGCTATGACTAAACCTCATTGGCAAGCAGAGAAAGAAGAAATCATAAAGCAGGGAGATAACTACCGTATCAAACCACGAGCACTTAATATTGTATGGGGCAATGATGAACGGTACTGGAAAATACCAGCCAGAGGGACTGAGGATATTCCTGCAGAGCTGGTACAGGTCTCGTGGCTGGAAGTAACAGGCTCAGTTGAGTTGAAAGCCGGGAAGGGGTACGAACTGACCTTCGATGTGGGACTGGCAGAAGATGCATTTGGTTGGAAAGACATACAAGTGTTCTTGATGGCAAAGGTAGGGAAAAAGGGAAAGTACAAATGGACAAGAGTTAAACTCGATCAGGATCAGAATAACGAGAGAGTTACGATTCCTGATAAACAGAAGCTTAGCATTGATGTTCCAGCTGATACAACGGACACCGGCCTTCATTTTGGTTTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATTTATAATGCGAAAGTAACTGCCACTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

170

Amino Acids

19.36

Weight (kDa)

8.61

Isoelectric Point (pI)

6.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 21 - 164 9.2e-20 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AclWI GGATC 1 cut(s) 354
AfaI GTAC 4 cut(s) 116, 165, 221, 317
AfiI CCNNNNNNNGG 2 cut(s) 134, 216
AgsI TTSAA 2 cut(s) 205, 478
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 3 cut(s) 160, 388, 407
AluI AGCT 3 cut(s) 160, 388, 407
Alw21I GWGCWC 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 175
AlwI GGATC 1 cut(s) 354
AoxI GGCC 1 cut(s) 424
AsuC2I CCSGG 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 52, 85
BauI CACGAG 2 cut(s) 78, 172
Bbv12I GWGCWC 1 cut(s) 85
BccI CCATC 1 cut(s) 283
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 175
BfmI CTRYAG 1 cut(s) 153
BlpI GCTNAGC 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu1102I GCTNAGC 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 211
BsaBI GATNNNNATC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 175
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 216
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 122, 249
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 134, 216
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 132, 207
BseNI ACTGG 2 cut(s) 122, 249
BshFI GGCC 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 85
BsiSI CCGG 2 cut(s) 210, 423
BslFI GGGAC 2 cut(s) 151, 255
BslI CCNNNNNNNGG 2 cut(s) 134, 216
BsmAI GTCTC 1 cut(s) 175
BsmFI GGGAC 2 cut(s) 151, 255
BsnI GGCC 1 cut(s) 426
Bso31I GGTCTC 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 85
Bsp143I GATC 2 cut(s) 340, 346
Bsp1720I GCTNAGC 1 cut(s) 389
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 2 cut(s) 133, 206
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 1 cut(s) 354
BspTNI GGTCTC 1 cut(s) 175
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 2 cut(s) 122, 249
BssAI RCCGGY 1 cut(s) 422
BssMI GATC 2 cut(s) 340, 346
BssSI CACGAG 2 cut(s) 78, 172
Bst2BI CACGAG 2 cut(s) 78, 172
Bst4CI ACNGT 2 cut(s) 68, 114
BstAPI GCANNNNNTGC 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 3 cut(s) 141, 193, 389
BstKTI GATC 2 cut(s) 343, 349
BstMAI GTCTC 1 cut(s) 175
BstMBI GATC 2 cut(s) 340, 346
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 209
BstSFI CTRYAG 1 cut(s) 153
BsuRI GGCC 1 cut(s) 426
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 422
Csp6I GTAC 4 cut(s) 115, 164, 220, 316
CviQI GTAC 4 cut(s) 115, 164, 220, 316
DdeI CTNAG 3 cut(s) 141, 193, 389
DpnI GATC 2 cut(s) 342, 348
DpnII GATC 2 cut(s) 340, 346
Eco31I GGTCTC 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 259
FaiI YATR 6 cut(s) 8, 47, 97, 275, 444, 486
FaqI GGGAC 2 cut(s) 151, 255
HaeIII GGCC 1 cut(s) 426
HapII CCGG 2 cut(s) 210, 423
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 1 cut(s) 370
HpaII CCGG 2 cut(s) 210, 423
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 3 cut(s) 286, 344, 374
HpyAV CCTTC 3 cut(s) 208, 241, 437
HpyCH4III ACNGT 2 cut(s) 68, 114
HpyCH4V TGCA 2 cut(s) 155, 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 3 cut(s) 141, 193, 389
Kzo9I GATC 2 cut(s) 340, 346
LweI GCATC 1 cut(s) 244
MaeIII GTNAC 3 cut(s) 184, 364, 496
MalI GATC 2 cut(s) 342, 348
MboI GATC 2 cut(s) 340, 346
MboII GAAGA 3 cut(s) 50, 263, 490
MhlI GDGCHC 1 cut(s) 85
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 4 cut(s) 27, 128, 136, 464
Mph1103I ATGCAT 1 cut(s) 259
MseI TTAA 2 cut(s) 87, 333
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 2 cut(s) 210, 423
MspR9I CCNGG 1 cut(s) 211
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 211
NdeII GATC 2 cut(s) 340, 346
NsiI ATGCAT 1 cut(s) 259
PfeI GAWTC 1 cut(s) 370
PsiI TTATAA 1 cut(s) 486
PstI CTGCAG 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 4 cut(s) 116, 165, 221, 317
RsaNI GTAC 4 cut(s) 115, 164, 220, 316
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 2 cut(s) 87, 333
Sau3AI GATC 2 cut(s) 340, 346
ScrFI CCNGG 1 cut(s) 211
SduI GDGCHC 1 cut(s) 85
SetI ASST 7 cut(s) 19, 162, 171, 233, 300, 390, 409
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 278
SspI AATATT 1 cut(s) 91
StyD4I CCNGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 68, 114
TaqI TCGA 2 cut(s) 234, 339
TatI WGTACW 1 cut(s) 315
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 87, 333
Tru9I TTAA 2 cut(s) 87, 333
TscAI CASTG 1 cut(s) 511
TspDTI ATGAA 3 cut(s) 123, 419, 459
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 1 cut(s) 511
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.