Rroxscaffold_1G00058250

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003915
Physical Location & Seq
Reverse (-)
80085852 .. 80087308
1457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058250.1

Sequence Viewer

Length: 528 bp
ATGTCTATGACTAAACCTCATTTCCAAGCAGAGAAAGATGAAGAGGCAGTCCAAAAGATTGGAGATGGCTACGATATCAAACCACGAGGACTTAACATTGTATGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAGGGCCTGCAGGTGTCAATGATGCTGCAGAGCTGGTGCAGGTTTCATGGCTGGAAGTAACAGGCTCAGTTGTTTTAACACCTGGGAAGACTTATAAACTGAACTTTGAGGTCCAACTGCTATCAGAGGCATTTGGCTGGAAAGATATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAAGGGAAAATACACGTGGAAAAGGGTTCAAGTACAGCAGCAGGCTGCGACAGACAAGTTCTTTATTCCTAAGGAAGAAGATGAGCTCCGCATTGCAGTTCCACCTGGGAATACTGACAACACCCTTCAGTTCGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATCTATAAAGCGAAAGTAACTGCGATCGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

175

Amino Acids

19.74

Weight (kDa)

8.91

Isoelectric Point (pI)

15.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 25 - 169 6.9e-22 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 2 cut(s) 140, 169
AciI CCGC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 428
AcvI CACGTG 1 cut(s) 333
AfaI GTAC 1 cut(s) 351
AfiI CCNNNNNNNGG 2 cut(s) 140, 312
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 2 cut(s) 347, 493
AjnI CCWGG 2 cut(s) 220, 421
AluBI AGCT 3 cut(s) 172, 293, 403
AluI AGCT 3 cut(s) 172, 293, 403
Alw21I GWGCWC 1 cut(s) 405
AoxI GGCC 2 cut(s) 143, 303
ApeKI GCWGC 3 cut(s) 164, 355, 362
AspS9I GGNCC 2 cut(s) 143, 250
AvaII GGWCC 1 cut(s) 250
AxyI CCTNAGG 1 cut(s) 387
BalI TGGCCA 1 cut(s) 305
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 84
BbrPI CACGTG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 3 cut(s) 151, 349, 367
BccI CCATC 2 cut(s) 59, 295
BciT130I CCWGG 2 cut(s) 222, 423
BfmI CTRYAG 2 cut(s) 147, 165
BfuAI ACCTGC 2 cut(s) 140, 169
BglII AGATCT 1 cut(s) 495
BisI GCNGC 3 cut(s) 165, 356, 363
BlsI GCNGC 3 cut(s) 166, 357, 364
Bme1390I CCNGG 2 cut(s) 222, 423
Bme18I GGWCC 1 cut(s) 250
BmgT120I GGNCC 2 cut(s) 143, 250
BmrFI CCNGG 2 cut(s) 222, 423
BmsI GCATC 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 333
BsaJI CCNNGG 3 cut(s) 221, 306, 422
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 312
Bse1I ACTGG 1 cut(s) 128
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 2 cut(s) 115, 408
BseBI CCWGG 2 cut(s) 222, 423
BseCI ATCGAT 1 cut(s) 522
BseDI CCNNGG 3 cut(s) 221, 306, 422
BseLI CCNNNNNNNGG 2 cut(s) 140, 312
BseMI GCAATG 2 cut(s) 115, 408
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 128
BseXI GCAGC 3 cut(s) 151, 349, 367
BsgI GTGCAG 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 522
BshFI GGCC 2 cut(s) 145, 305
BshVI ATCGAT 1 cut(s) 522
BsiEI CGRYCG 1 cut(s) 522
BsiHKAI GWGCWC 1 cut(s) 405
BslI CCNNNNNNNGG 2 cut(s) 140, 312
BsnI GGCC 2 cut(s) 145, 305
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 495, 519
BspACI CCGC 1 cut(s) 406
BspANI GGCC 2 cut(s) 145, 305
BspCNI CTCAG 1 cut(s) 218
BspDI ATCGAT 1 cut(s) 522
BspMAI CTGCAG 2 cut(s) 151, 169
BspMI ACCTGC 2 cut(s) 140, 169
BsrDI GCAATG 2 cut(s) 115, 408
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 3 cut(s) 221, 306, 422
BssMI GATC 2 cut(s) 495, 519
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 222, 423
Bst6I CTCTTC 1 cut(s) 36
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 147, 360
BstDEI CTNAG 2 cut(s) 205, 387
BstKTI GATC 2 cut(s) 498, 522
BstMBI GATC 2 cut(s) 495, 519
BstMCI CGRYCG 1 cut(s) 522
BstNI CCWGG 2 cut(s) 222, 423
BstSCI CCNGG 2 cut(s) 220, 421
BstSFI CTRYAG 2 cut(s) 147, 165
BstV1I GCAGC 3 cut(s) 151, 349, 367
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 1 cut(s) 495
Bsu15I ATCGAT 1 cut(s) 522
Bsu36I CCTNAGG 1 cut(s) 387
BsuRI GGCC 2 cut(s) 145, 305
BsuTUI ATCGAT 1 cut(s) 522
BveI ACCTGC 2 cut(s) 140, 169
Cac8I GCNNGC 2 cut(s) 147, 360
Cfr13I GGNCC 2 cut(s) 143, 250
ClaI ATCGAT 1 cut(s) 522
Csp6I GTAC 1 cut(s) 350
CviAII CATG 1 cut(s) 186
CviQI GTAC 1 cut(s) 350
DdeI CTNAG 2 cut(s) 205, 387
DpnI GATC 2 cut(s) 497, 521
DpnII GATC 2 cut(s) 495, 519
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 405
Eco32I GATATC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 250
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 428
Eco72I CACGTG 1 cut(s) 333
Eco81I CCTNAGG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 220, 421
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 189
FaiI YATR 7 cut(s) 8, 103, 187, 234, 287, 459, 501
FatI CATG 1 cut(s) 185
Fnu4HI GCNGC 3 cut(s) 165, 356, 363
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 3 cut(s) 165, 356, 363
GluI GCNGC 3 cut(s) 165, 356, 363
HaeIII GGCC 2 cut(s) 145, 305
Hin1II CATG 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 291
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 1 cut(s) 298
HpyAV CCTTC 1 cut(s) 452
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 4 cut(s) 149, 167, 178, 413
HpyF3I CTNAG 2 cut(s) 205, 387
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 1 cut(s) 189
Kzo9I GATC 2 cut(s) 495, 519
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 3 cut(s) 151, 349, 367
LweI GCATC 1 cut(s) 151
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 2 cut(s) 196, 511
MalI GATC 2 cut(s) 497, 521
MboI GATC 2 cut(s) 495, 519
MboII GAAGA 5 cut(s) 53, 238, 404, 407, 505
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 405
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 6 cut(s) 27, 37, 80, 241, 259, 479
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MseI TTAA 3 cut(s) 93, 215, 526
Msp20I TGGCCA 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 222, 423
MvaI CCWGG 2 cut(s) 222, 423
NdeII GATC 2 cut(s) 495, 519
NlaIII CATG 1 cut(s) 189
PaqCI CACCTGC 1 cut(s) 140
PkrI GCNGC 3 cut(s) 166, 357, 364
Ple19I CGATCG 1 cut(s) 522
PmaCI CACGTG 1 cut(s) 333
PmlI CACGTG 1 cut(s) 333
Ppu21I YACGTR 1 cut(s) 333
PsiI TTATAA 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 220, 421
PspCI CACGTG 1 cut(s) 333
PspGI CCWGG 2 cut(s) 220, 421
PspPI GGNCC 2 cut(s) 143, 250
PstI CTGCAG 2 cut(s) 151, 169
PsuI RGATCY 1 cut(s) 495
PvuI CGATCG 1 cut(s) 522
RsaI GTAC 1 cut(s) 351
RsaNI GTAC 1 cut(s) 350
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 3 cut(s) 93, 215, 526
SatI GCNGC 3 cut(s) 165, 356, 363
Sau3AI GATC 2 cut(s) 495, 519
Sau96I GGNCC 2 cut(s) 143, 250
SbfI CCTGCAGG 1 cut(s) 151
ScrFI CCNGG 2 cut(s) 222, 423
SdaI CCTGCAGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 405
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 2 cut(s) 147, 165
SinI GGWCC 1 cut(s) 250
Sse8387I CCTGCAGG 1 cut(s) 151
SsiI CCGC 1 cut(s) 406
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 220, 421
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 1 cut(s) 335
TaqI TCGA 1 cut(s) 522
TaqII GACCGA 1 cut(s) 440
TatI WGTACW 1 cut(s) 349
Tru1I TTAA 3 cut(s) 93, 215, 526
Tru9I TTAA 3 cut(s) 93, 215, 526
TseI GCWGC 3 cut(s) 164, 355, 362
TspDTI ATGAA 4 cut(s) 54, 129, 174, 474
VpaK11BI GGWCC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.