MD10G1234400.v1.1

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003915
Physical Location & Seq
Reverse (-)
33056980 .. 33058304
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1234400.v1.1.491

Sequence Viewer

Length: 543 bp
ATGTCTCTGAGTACTAAACCTCATTTCCAAGCAGAGGAAGATGAAATAACAAAACGGGGAGATAGTTACATCCTCAACCCACGGGGACTTAGCATTGTATGGGGCAATGTTGAACACCACTGGAAATTACCTAAGAAGCTATCCAAAGGTGACTCTAATGACCCTGCGGAGCTGAAACAGGTTTCTTGGCTAGAAGTAACCGGCTCAGTTAGTTTAATACCTACGAAGACGTACCAAATCAGCTTTGACGTAGAACTGACACCTTGTGCATTTGGTTGGAGAGACATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAGGGGCAAGTACAAATGGACCAAAGTTAAAGTAGTGCAGGATCCAAATGTAGGCAGATTCACAATTCCTGACAAAACCAGCCCGCCATTGAGAATTGAAGTTCTGCCCGCTAGTGTCGATAACATGCTTCACTTTGGTCTGTATGAAATCTGGAGTGGAAAATGGAAGGGGGGCTTGAAAATTCATCAAGCAAATGTGACAGAAGTAACTTCGACCTTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

181

Amino Acids

20.3

Weight (kDa)

9.26

Isoelectric Point (pI)

24.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 22 - 170 9.6e-20 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 167, 401, 426
AclWI GGATC 2 cut(s) 353, 366
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 1 cut(s) 498
AdeI CACNNNGTG 1 cut(s) 266
AfaI GTAC 3 cut(s) 13, 233, 329
AfiI CCNNNNNNNGG 3 cut(s) 34, 312, 368
AgsI TTSAA 3 cut(s) 113, 416, 496
AluBI AGCT 5 cut(s) 139, 172, 243, 293, 539
AluI AGCT 5 cut(s) 139, 172, 243, 293, 539
Alw26I GTCTC 2 cut(s) 9, 276
AlwI GGATC 2 cut(s) 353, 366
AoxI GGCC 1 cut(s) 303
ApoI RAATTY 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 336
BalI TGGCCA 1 cut(s) 305
BamHI GGATCC 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 233
BccI CCATC 1 cut(s) 295
BcoDI GTCTC 2 cut(s) 9, 276
BfaI CTAG 2 cut(s) 191, 429
BmcAI AGTACT 1 cut(s) 13
Bme18I GGWCC 1 cut(s) 336
BmgT120I GGNCC 1 cut(s) 336
BmiI GGNNCC 1 cut(s) 360
BpiI GAAGAC 1 cut(s) 233
BpmI CTGGAG 1 cut(s) 490
Bpu10I CCTNAGC 1 cut(s) 535
BsaJI CCNNGG 2 cut(s) 80, 306
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 312, 368
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 80, 306
BseGI GGATG 1 cut(s) 69
BseLI CCNNNNNNNGG 3 cut(s) 34, 312, 368
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 125
BsgI GTGCAG 1 cut(s) 374
BshFI GGCC 1 cut(s) 305
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 3 cut(s) 34, 312, 368
BsmAI GTCTC 2 cut(s) 9, 276
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 1 cut(s) 305
Bsp143I GATC 1 cut(s) 358
BspACI CCGC 3 cut(s) 167, 401, 426
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 1 cut(s) 218
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 2 cut(s) 353, 366
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 125
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 2 cut(s) 80, 306
BssMI GATC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 401, 426
BstDEI CTNAG 5 cut(s) 8, 89, 132, 205, 535
BstDSI CCRYGG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 69
BstKTI GATC 1 cut(s) 361
BstMAI GTCTC 2 cut(s) 9, 276
BstMBI GATC 1 cut(s) 358
BstNSI RCATGY 1 cut(s) 445
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 2 cut(s) 401, 426
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 336
Csp6I GTAC 3 cut(s) 12, 232, 328
CviAII CATG 1 cut(s) 442
CviQI GTAC 3 cut(s) 12, 232, 328
DdeI CTNAG 5 cut(s) 8, 89, 132, 205, 535
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
DraIII CACNNNGTG 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 445
FaiI YATR 4 cut(s) 100, 287, 443, 462
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FaqI GGGAC 1 cut(s) 99
FatI CATG 1 cut(s) 441
FauI CCCGC 2 cut(s) 408, 433
FokI GGATG 1 cut(s) 56
FspBI CTAG 2 cut(s) 191, 429
GsuI CTGGAG 1 cut(s) 490
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 201
Hin1II CATG 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 2 cut(s) 152, 375
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 3 cut(s) 298, 386, 469
HpyAV CCTTC 1 cut(s) 478
HpyCH4IV ACGT 2 cut(s) 230, 249
HpyCH4V TGCA 2 cut(s) 269, 355
HpyF3I CTNAG 5 cut(s) 8, 89, 132, 205, 535
HpySE526I ACGT 2 cut(s) 230, 249
Hsp92II CATG 1 cut(s) 445
Kzo9I GATC 1 cut(s) 358
LmnI GCTCC 1 cut(s) 169
LpnPI CCDG 8 cut(s) 106, 164, 177, 214, 341, 399, 409, 454
MaeI CTAG 2 cut(s) 191, 429
MaeII ACGT 2 cut(s) 230, 249
MaeIII GTNAC 5 cut(s) 65, 149, 196, 514, 523
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 2 cut(s) 50, 238
MflI RGATCY 1 cut(s) 358
MlsI TGGCCA 1 cut(s) 305
MluCI AATT 4 cut(s) 125, 381, 411, 498
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 3 cut(s) 28, 30, 83
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MseI TTAA 3 cut(s) 215, 345, 541
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 1 cut(s) 201
NdeII GATC 1 cut(s) 358
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 1 cut(s) 360
NmuCI GTSAC 2 cut(s) 149, 514
NspI RCATGY 1 cut(s) 445
PfeI GAWTC 1 cut(s) 375
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 336
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 3 cut(s) 13, 233, 329
RsaNI GTAC 3 cut(s) 12, 232, 328
SaqAI TTAA 3 cut(s) 215, 345, 541
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 1 cut(s) 336
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 1 cut(s) 146
SinI GGWCC 1 cut(s) 336
Sse9I AATT 4 cut(s) 125, 381, 411, 498
SsiI CCGC 3 cut(s) 167, 401, 426
SspMI CTAG 2 cut(s) 191, 429
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 2 cut(s) 233, 252
TaqI TCGA 2 cut(s) 435, 530
TasI AATT 4 cut(s) 125, 381, 411, 498
TatI WGTACW 2 cut(s) 11, 327
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 3 cut(s) 215, 345, 541
Tru9I TTAA 3 cut(s) 215, 345, 541
TscAI CASTG 1 cut(s) 125
TseFI GTSAC 2 cut(s) 149, 514
Tsp45I GTSAC 2 cut(s) 149, 514
TspDTI ATGAA 3 cut(s) 57, 477, 491
TspRI CASTG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 336
XapI RAATTY 1 cut(s) 498
XceI RCATGY 1 cut(s) 445
XspI CTAG 2 cut(s) 191, 429
ZrmI AGTACT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.