Rw5G012850

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003915
Physical Location & Seq
Forward (+)
15242059 .. 15243223
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012850.1

Sequence Viewer

Length: 519 bp
ATGTCTATGACTAAACCTCATTTCCAAGCAGTGAAAGATGAACAGACAGTCCTGCCAAAGACTGGAGGATCAGCGGGCTATACTATCAAACCACAAGGACTTAACATAGTATGGGGCAATGATGAACGCTACTGGAAAATACCTGCCAAAGGCACCAATGAGGCTGCAGAGCTGGTGCAGGTTTCATGGCTTGAAGTAACAGGCTCAGTTGGTTTAATAGCTGGGAAGAAGTATAAACTGAACTTTGAGGTAGAACTGCTACCAGAGGCATTTGGCTGGAAAGATATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAAGGGAAGGTACAACTGGAAAAGGGTTCAACTGCAGCCGGGGACGGAAAAAACCTTGATTCCTGAGGGAGATACGCTCACCATTGAAGTTCCAACTGGGCAAAATGTCGACACCACCCTTCAGTTCGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATTTATGATGCGCAAGTAACTGAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

172

Amino Acids

19.2

Weight (kDa)

8.65

Isoelectric Point (pI)

18.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 27 - 165 7.3e-20 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 2 cut(s) 151, 169
AccB1I GGYRCC 1 cut(s) 152
AccB7I CCANNNNNTGG 1 cut(s) 62
AccI GTMKAC 1 cut(s) 426
AciI CCGC 1 cut(s) 74
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 3 cut(s) 62, 149, 312
AgsI TTSAA 4 cut(s) 194, 347, 404, 487
AloI GAACNNNNNNTCC 2 cut(s) 33, 65
AluBI AGCT 3 cut(s) 172, 221, 293
AluI AGCT 3 cut(s) 172, 221, 293
AlwI GGATC 1 cut(s) 76
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 164, 352
AspLEI GCGC 1 cut(s) 502
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 388
AxyI CCTNAGG 1 cut(s) 381
BalI TGGCCA 1 cut(s) 305
BanI GGYRCC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 151, 364
BccI CCATC 1 cut(s) 295
BcnI CCSGG 1 cut(s) 357
BfmI CTRYAG 2 cut(s) 165, 350
BfuAI ACCTGC 2 cut(s) 151, 169
BisI GCNGC 2 cut(s) 165, 353
BlsI GCNGC 2 cut(s) 166, 354
Bme1390I CCNGG 1 cut(s) 357
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 357
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 1 cut(s) 487
BmuI ACTGGG 1 cut(s) 423
BplI GAGNNNNNCTC 2 cut(s) 378, 410
BpmI CTGGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 306, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 149, 312
Bse1I ACTGG 4 cut(s) 67, 137, 338, 418
Bse21I CCTNAGG 1 cut(s) 381
Bse3DI GCAATG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 306, 356
BseLI CCNNNNNNNGG 3 cut(s) 62, 149, 312
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 3 cut(s) 219, 372, 501
BseNI ACTGG 4 cut(s) 67, 137, 338, 418
BseXI GCAGC 2 cut(s) 151, 364
BseYI CCCAGC 1 cut(s) 221
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 152
BsiSI CCGG 1 cut(s) 356
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 3 cut(s) 62, 149, 312
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 1 cut(s) 305
Bsp143I GATC 1 cut(s) 68
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 3 cut(s) 218, 373, 502
BspLI GGNNCC 1 cut(s) 154
BspMAI CTGCAG 2 cut(s) 169, 354
BspMI ACCTGC 2 cut(s) 151, 169
BspPI GGATC 1 cut(s) 76
BspT107I GGYRCC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 124
BsrI ACTGG 4 cut(s) 67, 137, 338, 418
BssECI CCNNGG 2 cut(s) 306, 356
BssMI GATC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 3 cut(s) 205, 381, 510
BstENI CCTNNNNNAGG 1 cut(s) 147
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 1 cut(s) 71
BstMBI GATC 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 355
BstSFI CTRYAG 2 cut(s) 165, 350
BstV1I GCAGC 2 cut(s) 151, 364
Bsu36I CCTNAGG 1 cut(s) 381
BsuRI GGCC 1 cut(s) 305
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
BveI ACCTGC 2 cut(s) 151, 169
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 502
Csp6I GTAC 1 cut(s) 328
CviAII CATG 1 cut(s) 186
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 3 cut(s) 205, 381, 510
DpnI GATC 1 cut(s) 70
DpnII GATC 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 147
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 189
FaiI YATR 9 cut(s) 8, 81, 107, 112, 187, 234, 287, 453, 495
FaqI GGGAC 1 cut(s) 373
FatI CATG 1 cut(s) 185
FauI CCCGC 1 cut(s) 67
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 2 cut(s) 165, 353
Fsp4HI GCNGC 2 cut(s) 165, 353
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 1 cut(s) 501
GluI GCNGC 2 cut(s) 165, 353
GsaI CCCAGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 84
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 356
HhaI GCGC 1 cut(s) 502
Hin1II CATG 1 cut(s) 189
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 1 cut(s) 376
HpaII CCGG 1 cut(s) 356
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 1 cut(s) 427
Hpy188III TCNNGA 2 cut(s) 298, 380
Hpy8I GTNNAC 1 cut(s) 427
HpyAV CCTTC 2 cut(s) 318, 446
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 3 cut(s) 167, 178, 352
HpyF3I CTNAG 3 cut(s) 205, 381, 510
Hsp92II CATG 1 cut(s) 189
HspAI GCGC 1 cut(s) 500
Kzo9I GATC 1 cut(s) 68
Lsp1109I GCAGC 2 cut(s) 151, 364
LweI GCATC 1 cut(s) 487
MaeIII GTNAC 2 cut(s) 196, 505
MalI GATC 1 cut(s) 70
MboI GATC 1 cut(s) 68
MboII GAAGA 2 cut(s) 238, 499
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 434
MnlI CCTC 7 cut(s) 27, 59, 154, 241, 259, 376, 473
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MseI TTAA 3 cut(s) 102, 215, 517
Msp20I TGGCCA 1 cut(s) 305
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 357
NciI CCSGG 1 cut(s) 357
NdeII GATC 1 cut(s) 68
NlaIII CATG 1 cut(s) 189
NlaIV GGNNCC 1 cut(s) 154
NsbI TGCGCA 1 cut(s) 501
PfeI GAWTC 1 cut(s) 376
PflMI CCANNNNNTGG 1 cut(s) 62
PkrI GCNGC 2 cut(s) 166, 354
PspFI CCCAGC 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 154
PstI CTGCAG 2 cut(s) 169, 354
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 3 cut(s) 102, 215, 517
SatI GCNGC 2 cut(s) 165, 353
Sau3AI GATC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 357
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 2 cut(s) 165, 350
SsiI CCGC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 1 cut(s) 426
TaqII GACCGA 1 cut(s) 434
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 3 cut(s) 102, 215, 517
Tru9I TTAA 3 cut(s) 102, 215, 517
TscAI CASTG 1 cut(s) 36
TseI GCWGC 2 cut(s) 164, 352
TspDTI ATGAA 4 cut(s) 54, 138, 174, 468
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 36
Van91I CCANNNNNTGG 1 cut(s) 62
XagI CCTNNNNNAGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.