RLG00000032487

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003915
Physical Location & Seq
Forward (+)
15772527 .. 15773687
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032487

Sequence Viewer

Length: 528 bp
ATGTCTATGACTAAACCTCATTTCCAAGCAGAGAAAGATGAAGCGACAGAGACTGAAGATGGCGGCTACATTATCAAACCACGAGGACTTAACATTGTATGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAGGGCCTGCAGGTGTCAATGATGCTGCAGAGCTGGTGCAGGTTTCATGGCTGGAAGTAACAGGCTCAGTTGTTTTAACACCTGGGAAGACTTATAAACTGAACTTTGAGGTACAACTGCTATCAGAGGCATTTGGCTGGAAAGATATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAAGGGAAGATACACGTGGAAAAGGGTTCAAGTACAGCAGCAGGCTGGGACAGACAAATTCTTGATTCCTAAGAAAGAAGATGAGCTCCGCATTGAAGTTCCACCTGCGAATACTGACAACACCCTTCAGTTCGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATCTATAATGCGCGAGTAACTGCGATTAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

176

Amino Acids

19.76

Weight (kDa)

8.96

Isoelectric Point (pI)

18.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 23 - 166 4e-22 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 231
AarI CACCTGC 2 cut(s) 137, 427
Acc36I ACCTGC 3 cut(s) 137, 166, 427
AccII CGCG 1 cut(s) 505
AciI CCGC 2 cut(s) 63, 403
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 2 cut(s) 75, 425
AcvI CACGTG 1 cut(s) 330
AfaI GTAC 2 cut(s) 249, 348
AfiI CCNNNNNNNGG 2 cut(s) 137, 309
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 3 cut(s) 344, 410, 490
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 3 cut(s) 169, 290, 400
AluI AGCT 3 cut(s) 169, 290, 400
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 2 cut(s) 140, 300
ApeKI GCWGC 2 cut(s) 161, 352
ApoI RAATTY 1 cut(s) 371
AseI ATTAAT 1 cut(s) 519
AspLEI GCGC 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 140
BalI TGGCCA 1 cut(s) 302
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 402
BbvI GCAGC 2 cut(s) 148, 364
BccI CCATC 2 cut(s) 53, 292
BciT130I CCWGG 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 44
BfmI CTRYAG 2 cut(s) 144, 162
BfuAI ACCTGC 3 cut(s) 137, 166, 427
BglII AGATCT 1 cut(s) 492
BisI GCNGC 3 cut(s) 64, 162, 353
BlsI GCNGC 3 cut(s) 65, 163, 354
Bme1390I CCNGG 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 219
BmsI GCATC 1 cut(s) 148
BpiI GAAGAC 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 330
BsaJI CCNNGG 2 cut(s) 218, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 309
Bse1I ACTGG 1 cut(s) 125
Bse3DI GCAATG 1 cut(s) 112
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 218, 303
BseLI CCNNNNNNNGG 2 cut(s) 137, 309
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 125
BseXI GCAGC 2 cut(s) 148, 364
BseYI CCCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 194
Bsh1236I CGCG 1 cut(s) 505
BshFI GGCC 2 cut(s) 142, 302
BsiHKAI GWGCWC 1 cut(s) 402
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 2 cut(s) 137, 309
BsmAI GTCTC 1 cut(s) 44
BsmFI GGGAC 1 cut(s) 376
BsnI GGCC 2 cut(s) 142, 302
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 1 cut(s) 492
BspACI CCGC 2 cut(s) 63, 403
BspANI GGCC 2 cut(s) 142, 302
BspCNI CTCAG 1 cut(s) 215
BspFNI CGCG 1 cut(s) 505
BspMAI CTGCAG 2 cut(s) 148, 166
BspMI ACCTGC 3 cut(s) 137, 166, 427
BsrDI GCAATG 1 cut(s) 112
BsrI ACTGG 1 cut(s) 125
BssECI CCNNGG 2 cut(s) 218, 303
BssMI GATC 1 cut(s) 492
BssSI CACGAG 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 219
BstBAI YACGTR 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 144, 357
BstDEI CTNAG 2 cut(s) 202, 384
BstFNI CGCG 1 cut(s) 505
BstHHI GCGC 1 cut(s) 505
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 1 cut(s) 44
BstMBI GATC 1 cut(s) 492
BstNI CCWGG 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 2 cut(s) 144, 162
BstUI CGCG 1 cut(s) 505
BstV1I GCAGC 2 cut(s) 148, 364
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BsuRI GGCC 2 cut(s) 142, 302
BveI ACCTGC 3 cut(s) 137, 166, 427
Cac8I GCNNGC 2 cut(s) 144, 357
CaiI CAGNNNCTG 1 cut(s) 53
CfoI GCGC 1 cut(s) 505
Cfr13I GGNCC 1 cut(s) 140
Csp6I GTAC 2 cut(s) 248, 347
CviAII CATG 1 cut(s) 183
CviQI GTAC 2 cut(s) 248, 347
DdeI CTNAG 2 cut(s) 202, 384
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 2 cut(s) 75, 425
Eco72I CACGTG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 400
EcoO109I RGGNCCY 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 186
FaiI YATR 7 cut(s) 8, 100, 184, 231, 284, 456, 498
FaqI GGGAC 1 cut(s) 376
FatI CATG 1 cut(s) 182
Fnu4HI GCNGC 3 cut(s) 64, 162, 353
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 3 cut(s) 64, 162, 353
GlaI GCGC 1 cut(s) 504
GluI GCNGC 3 cut(s) 64, 162, 353
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 2 cut(s) 142, 302
HhaI GCGC 1 cut(s) 505
Hin1II CATG 1 cut(s) 186
Hin6I GCGC 1 cut(s) 503
HinP1I GCGC 1 cut(s) 503
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 1 cut(s) 379
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 2 cut(s) 295, 376
HpyAV CCTTC 1 cut(s) 449
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 3 cut(s) 146, 164, 175
HpyF3I CTNAG 2 cut(s) 202, 384
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 1 cut(s) 186
HspAI GCGC 1 cut(s) 503
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 2 cut(s) 148, 364
LweI GCATC 1 cut(s) 148
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 2 cut(s) 193, 508
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 5 cut(s) 68, 235, 333, 404, 502
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 1 cut(s) 402
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 1 cut(s) 371
MluNI TGGCCA 1 cut(s) 302
MnlI CCTC 5 cut(s) 27, 77, 238, 256, 476
Mox20I TGGCCA 1 cut(s) 302
MscI TGGCCA 1 cut(s) 302
MseI TTAA 3 cut(s) 90, 212, 519
Msp20I TGGCCA 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 219
MvnI CGCG 1 cut(s) 505
NdeII GATC 1 cut(s) 492
NlaIII CATG 1 cut(s) 186
PaqCI CACCTGC 2 cut(s) 137, 427
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 3 cut(s) 65, 163, 354
PmaCI CACGTG 1 cut(s) 330
PmlI CACGTG 1 cut(s) 330
Ppu21I YACGTR 1 cut(s) 330
PshBI ATTAAT 1 cut(s) 519
PsiI TTATAA 1 cut(s) 231
Psp124BI GAGCTC 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 217
PspCI CACGTG 1 cut(s) 330
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 217
PspPI GGNCC 1 cut(s) 140
PstI CTGCAG 2 cut(s) 148, 166
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 492
RsaI GTAC 2 cut(s) 249, 348
RsaNI GTAC 2 cut(s) 248, 347
SacI GAGCTC 1 cut(s) 402
SaqAI TTAA 3 cut(s) 90, 212, 519
SatI GCNGC 3 cut(s) 64, 162, 353
Sau3AI GATC 1 cut(s) 492
Sau96I GGNCC 1 cut(s) 140
SbfI CCTGCAGG 1 cut(s) 148
ScrFI CCNGG 1 cut(s) 219
SdaI CCTGCAGG 1 cut(s) 148
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 2 cut(s) 144, 162
Sse8387I CCTGCAGG 1 cut(s) 148
Sse9I AATT 1 cut(s) 371
SsiI CCGC 2 cut(s) 63, 403
SstI GAGCTC 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 303
TaiI ACGT 1 cut(s) 332
TaqII GACCGA 1 cut(s) 437
TasI AATT 1 cut(s) 371
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 3 cut(s) 90, 212, 519
Tru9I TTAA 3 cut(s) 90, 212, 519
TseI GCWGC 2 cut(s) 161, 352
TspDTI ATGAA 4 cut(s) 54, 126, 171, 471
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.