MD10G1234200.v1.1

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003915
Physical Location & Seq
Reverse (-)
33021758 .. 33022380
623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1234200.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGGAGCTACGCGTCTACTCCACTTATGTGTGCTTGTCCCGCCTTTTGAGATTTTTGTTTGGCTATCCAAAGGTGACTCTAATGACCCTACGGAGTTGAAACAGGTTTCTTGGCTAGAAGTAACAGGCTCAGTTAGTTTAATACCTACGAAGACGTACCAAATCAGCTTAGACGTAGAAATGGCACCTAGTTCATTTGGTTGGAGAGATATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAGGGGCAAGTACAAATGGACCAAAGTTAAAGTAGTGCAGGATCCAAATGTAGGCAGATTCACAATTCCTGACAAAACCAGCCCGCCATTGAGAATTGAAGTTCTGCCCGCTAGTGTCGATAACATGCTTCACTTTGGTCTGTATGAAATCTGGAGTGGAAAATGGAAGGGGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

140

Amino Acids

15.66

Weight (kDa)

9.27

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 28 - 138 1.7e-09 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccI GTMKAC 1 cut(s) 16
AccII CGCG 1 cut(s) 13
AciI CCGC 3 cut(s) 41, 326, 351
AclWI GGATC 2 cut(s) 278, 291
AcoI YGGCCR 1 cut(s) 228
AfaI GTAC 2 cut(s) 158, 254
AfiI CCNNNNNNNGG 2 cut(s) 237, 293
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 2 cut(s) 100, 341
AleI CACNNNNGTG 1 cut(s) 27
AluBI AGCT 3 cut(s) 8, 168, 218
AluI AGCT 3 cut(s) 8, 168, 218
AlwI GGATC 2 cut(s) 278, 291
AoxI GGCC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 230
BamHI GGATCC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 158
BccI CCATC 1 cut(s) 220
BfaI CTAG 3 cut(s) 116, 189, 354
Bme18I GGWCC 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 186, 285
BpiI GAAGAC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 415
BsaJI CCNNGG 1 cut(s) 231
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 293
BseDI CCNNGG 1 cut(s) 231
BseLI CCNNNNNNNGG 2 cut(s) 237, 293
BseMII CTCAG 1 cut(s) 144
BsgI GTGCAG 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 13
BshFI GGCC 1 cut(s) 230
BshNI GGYRCC 1 cut(s) 184
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 2 cut(s) 237, 293
BsmFI GGGAC 1 cut(s) 23
BsnI GGCC 1 cut(s) 230
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 3 cut(s) 41, 326, 351
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 143
BspFNI CGCG 1 cut(s) 13
BspLI GGNNCC 2 cut(s) 186, 285
BspPI GGATC 2 cut(s) 278, 291
BspT107I GGYRCC 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 326, 351
BstDEI CTNAG 2 cut(s) 130, 169
BstFNI CGCG 1 cut(s) 13
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNSI RCATGY 1 cut(s) 370
BstUI CGCG 1 cut(s) 13
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 1 cut(s) 230
Cac8I GCNNGC 2 cut(s) 326, 351
Cfr13I GGNCC 1 cut(s) 261
CseI GACGC 1 cut(s) 2
Csp6I GTAC 2 cut(s) 157, 253
CviAII CATG 1 cut(s) 367
CviJI RGCY 9 cut(s) 8, 64, 115, 129, 168, 218, 230, 324, 416
CviKI_1 RGCY 9 cut(s) 8, 64, 115, 129, 168, 218, 230, 324, 416
CviQI GTAC 2 cut(s) 157, 253
DdeI CTNAG 2 cut(s) 130, 169
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
EaeI YGGCCR 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 1 cut(s) 370
FaiI YATR 4 cut(s) 28, 212, 368, 387
FaqI GGGAC 1 cut(s) 23
FatI CATG 1 cut(s) 366
FauI CCCGC 3 cut(s) 48, 333, 358
FblI GTMKAC 1 cut(s) 16
FspBI CTAG 3 cut(s) 116, 189, 354
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 1 cut(s) 230
HgaI GACGC 1 cut(s) 2
Hin1II CATG 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 2 cut(s) 77, 300
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 1 cut(s) 17
Hpy188III TCNNGA 3 cut(s) 223, 311, 394
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 403
HpyCH4IV ACGT 2 cut(s) 155, 174
HpyCH4V TGCA 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 2 cut(s) 130, 169
HpySE526I ACGT 2 cut(s) 155, 174
Hsp92II CATG 1 cut(s) 370
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 5
LpnPI CCDG 6 cut(s) 89, 111, 266, 324, 334, 379
MaeI CTAG 3 cut(s) 116, 189, 354
MaeII ACGT 2 cut(s) 155, 174
MaeIII GTNAC 2 cut(s) 74, 121
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 1 cut(s) 163
MflI RGATCY 1 cut(s) 283
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 2 cut(s) 306, 336
MluI ACGCGT 1 cut(s) 11
MluNI TGGCCA 1 cut(s) 230
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 182
Mox20I TGGCCA 1 cut(s) 230
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 140, 270
MslI CAYNNNNRTG 1 cut(s) 27
Msp20I TGGCCA 1 cut(s) 230
MvnI CGCG 1 cut(s) 13
MwoI GCNNNNNNNGC 1 cut(s) 40
NdeII GATC 1 cut(s) 283
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 2 cut(s) 186, 285
NmuCI GTSAC 1 cut(s) 74
NspI RCATGY 1 cut(s) 370
OliI CACNNNNGTG 1 cut(s) 27
PfeI GAWTC 1 cut(s) 300
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 186, 285
PspPI GGNCC 1 cut(s) 261
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 2 cut(s) 158, 254
RsaNI GTAC 2 cut(s) 157, 253
RseI CAYNNNNRTG 1 cut(s) 27
SaqAI TTAA 2 cut(s) 140, 270
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 1 cut(s) 71
SinI GGWCC 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 27
Sse9I AATT 2 cut(s) 306, 336
SsiI CCGC 3 cut(s) 41, 326, 351
SspMI CTAG 3 cut(s) 116, 189, 354
StyI CCWWGG 1 cut(s) 231
TaiI ACGT 2 cut(s) 158, 177
TaqI TCGA 1 cut(s) 360
TasI AATT 2 cut(s) 306, 336
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 300
Tru1I TTAA 2 cut(s) 140, 270
Tru9I TTAA 2 cut(s) 140, 270
TseFI GTSAC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 2 cut(s) 183, 402
TspGWI ACGGA 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 261
XceI RCATGY 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 16
XspI CTAG 3 cut(s) 116, 189, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.