Rorug05G0055300

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003915
Physical Location & Seq
Reverse (-)
4884151 .. 4886650
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0055300.1

Sequence Viewer

Length: 1092 bp
ATGGTGGATGTGGCTGCTGCTGGTGCGGGGAACGCAAGTAAGGATCCAAATTTGATCCCACCACCTCCTCCAACCATTCCACCCAATGTGAAGCCAGGAGTCCTGACTTCTCCCAAGTATTCTATTACGAGTAGGAAACAATTGCTTGCAAACCACTTCAAACTCACTGTGAAAGCTCCAGATTCTGTATTCTACCAATATTCTGTTCATATTACTTCTGAAAACAAGAAGGCCACTCAAGGCAAGGGAATTGGCAGAAAACTGGTTGACAGGCTCTATCAAATATATTCTTCTAAACTTGCTGATAACAAGTTTGTGTATGATAAAGAGAAAGCTCTATATACAGTTGGTCCTCTGCCACAGAACAAGCCTGACTTTACAGTTCCCCTGGAGGAGACATTTGCAAAGAGTGAAAAAGTGGAGCCTAACAATACATACTTAAAGAGATTTGCGAATTTAAAGATTCAGTTCAAAGAAAAATGTAAGCCATGCATTACCAGTATAATGGACTTCAATTTTGTTCAAGCACCTGGATGCCATCAACAACCATACTCAACTTTACAATGTCACAAAAGAACTTCAGCCCGTCTTTTCCAACAAACTGCATTACAACTCACTAAGCAGATATTCCGGAAGGAATTCAAGAAGTTTACAAGGAAGTTCAGTTGGCAGTGCTGTTTGTATGCGCTTGACGAGGTGAGTATTATTCCTTCATGGTCAAAGCTTATGCTCGGTGGCTCAGCCAGCCCCAGCCCCTCCAATTATTTGAATTTGCTATTAGTTGTCTATATCTTTAAGTTTCAGTTTCTAATTCCCGCAGCAAAGCGCGGGCGACTATTACTAGTTGCACTCATAGTTAAGGAAAGGCTTCCTGCTGAGCCTGTCAAGAAGAAAAAAAGTGCAGACTGGAAAACTCTGAATTTGGACAAGGCTGATGATATGTTTTCTGATTTCTATTTAGTTGCTCACTCAAAAATATACATCATGGCCTATCAAGTTGCTCAGACTATGGAAATGGTAACTACAAACAGCTATACAAATTTGTCAAACATTGAAACTGACTGGAGGCTTGTAGTTATGCTCCTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

363

Amino Acids

41.29

Weight (kDa)

9.65

Isoelectric Point (pI)

32.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 47 - 160 2.2e-17 N-terminal domain of argonaute
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 828
AccIII TCCGGA 1 cut(s) 630
AciI CCGC 3 cut(s) 26, 816, 828
AclWI GGATC 3 cut(s) 38, 49, 51
AcsI RAATTY 6 cut(s) 49, 454, 638, 769, 919, 1039
AcuI CTGAAG 1 cut(s) 564
AgsI TTSAA 7 cut(s) 160, 472, 514, 524, 643, 769, 1055
AhlI ACTAGT 1 cut(s) 841
AjnI CCWGG 3 cut(s) 94, 387, 529
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AluBI AGCT 4 cut(s) 176, 335, 724, 1032
AluI AGCT 4 cut(s) 176, 335, 724, 1032
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 3 cut(s) 38, 49, 51
AlwNI CAGNNNCTG 1 cut(s) 185
Aor13HI TCCGGA 1 cut(s) 630
AoxI GGCC 2 cut(s) 231, 987
ApeKI GCWGC 3 cut(s) 14, 17, 818
ApoI RAATTY 6 cut(s) 49, 454, 638, 769, 919, 1039
ArsI GACNNNNNNTTYG 2 cut(s) 500, 532
Asp700I GAANNNNTTC 2 cut(s) 638, 867
AspLEI GCGC 2 cut(s) 688, 828
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 1 cut(s) 709
AvaII GGWCC 1 cut(s) 350
BamHI GGATCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 694, 726
BbvI GCAGC 2 cut(s) 4, 830
BccI CCATC 1 cut(s) 546
BciT130I CCWGG 3 cut(s) 96, 389, 531
BcoDI GTCTC 1 cut(s) 389
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 1 cut(s) 842
BisI GCNGC 3 cut(s) 15, 18, 819
BlpI GCTNAGC 2 cut(s) 739, 876
BlsI GCNGC 3 cut(s) 16, 19, 820
Bme1390I CCNGG 3 cut(s) 96, 389, 531
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 2 cut(s) 45, 423
BmrFI CCNGG 3 cut(s) 96, 389, 531
BmsI GCATC 1 cut(s) 524
BpmI CTGGAG 3 cut(s) 162, 410, 1084
Bpu1102I GCTNAGC 2 cut(s) 739, 876
BpuEI CTTGAG 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 387
BsaWI WCCGGW 1 cut(s) 630
Bse1I ACTGG 4 cut(s) 267, 498, 911, 1067
BseAI TCCGGA 1 cut(s) 630
BseBI CCWGG 3 cut(s) 96, 389, 531
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 2 cut(s) 13, 539
BseMII CTCAG 3 cut(s) 753, 867, 1016
BseNI ACTGG 4 cut(s) 267, 498, 911, 1067
BseRI GAGGAG 2 cut(s) 57, 407
BseXI GCAGC 2 cut(s) 4, 830
BseYI CCCAGC 1 cut(s) 749
BsgI GTGCAG 1 cut(s) 921
Bsh1236I CGCG 1 cut(s) 828
BshFI GGCC 2 cut(s) 233, 989
BsiSI CCGG 1 cut(s) 631
BsmAI GTCTC 1 cut(s) 389
BsnI GGCC 2 cut(s) 233, 989
Bsp13I TCCGGA 1 cut(s) 630
Bsp143I GATC 2 cut(s) 43, 54
Bsp1720I GCTNAGC 2 cut(s) 739, 876
BspACI CCGC 3 cut(s) 26, 816, 828
BspANI GGCC 2 cut(s) 233, 989
BspCNI CTCAG 3 cut(s) 752, 868, 1015
BspEI TCCGGA 1 cut(s) 630
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 2 cut(s) 45, 423
BspPI GGATC 3 cut(s) 38, 49, 51
BsrI ACTGG 4 cut(s) 267, 498, 911, 1067
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 43, 54
Bst2UI CCWGG 3 cut(s) 96, 389, 531
Bst4CI ACNGT 3 cut(s) 169, 346, 382
BstC8I GCNNGC 3 cut(s) 147, 745, 830
BstDEI CTNAG 4 cut(s) 618, 739, 876, 1002
BstF5I GGATG 2 cut(s) 13, 539
BstFNI CGCG 1 cut(s) 828
BstHHI GCGC 2 cut(s) 688, 828
BstKTI GATC 2 cut(s) 46, 57
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 2 cut(s) 43, 54
BstMWI GCNNNNNNNGC 3 cut(s) 23, 32, 744
BstNI CCWGG 3 cut(s) 96, 389, 531
BstSCI CCNGG 3 cut(s) 94, 387, 529
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 2 cut(s) 4, 830
BstX2I RGATCY 1 cut(s) 43
BstXI CCANNNNNNTGG 1 cut(s) 505
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 2 cut(s) 233, 989
BtsCI GGATG 2 cut(s) 13, 539
BtsI GCAGTG 1 cut(s) 677
BtsIMutI CAGTG 2 cut(s) 165, 677
Cac8I GCNNGC 3 cut(s) 147, 745, 830
CaiI CAGNNNCTG 1 cut(s) 185
CfoI GCGC 2 cut(s) 688, 828
Cfr13I GGNCC 1 cut(s) 350
CviAII CATG 3 cut(s) 489, 714, 985
DdeI CTNAG 4 cut(s) 618, 739, 876, 1002
DpnI GATC 2 cut(s) 45, 56
DpnII GATC 2 cut(s) 43, 54
DraI TTTAAA 1 cut(s) 459
Eco47I GGWCC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 3 cut(s) 94, 387, 529
EcoT22I ATGCAT 1 cut(s) 494
FaeI CATG 3 cut(s) 492, 717, 988
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FatI CATG 3 cut(s) 488, 713, 984
FauI CCCGC 3 cut(s) 19, 821, 823
Fnu4HI GCNGC 3 cut(s) 15, 18, 819
FokI GGATG 2 cut(s) 20, 546
Fsp4HI GCNGC 3 cut(s) 15, 18, 819
FspBI CTAG 1 cut(s) 842
GlaI GCGC 2 cut(s) 687, 827
GluI GCNGC 3 cut(s) 15, 18, 819
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 3 cut(s) 162, 410, 1084
HaeIII GGCC 2 cut(s) 233, 989
HapII CCGG 1 cut(s) 631
HhaI GCGC 2 cut(s) 688, 828
Hin1II CATG 3 cut(s) 492, 717, 988
Hin6I GCGC 2 cut(s) 686, 826
HinP1I GCGC 2 cut(s) 686, 826
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 722
HinfI GANTC 3 cut(s) 99, 182, 463
HpaII CCGG 1 cut(s) 631
HphI GGTGA 1 cut(s) 709
Hpy166II GTNNAC 2 cut(s) 268, 651
Hpy188I TCNGA 4 cut(s) 220, 918, 949, 1005
Hpy188III TCNNGA 5 cut(s) 103, 179, 631, 643, 886
Hpy8I GTNNAC 2 cut(s) 268, 651
HpyAV CCTTC 3 cut(s) 223, 628, 720
HpyCH4III ACNGT 3 cut(s) 169, 346, 382
HpyCH4V TGCA 6 cut(s) 149, 404, 492, 605, 848, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 32, 744
HpyF3I CTNAG 4 cut(s) 618, 739, 876, 1002
Hsp92II CATG 3 cut(s) 492, 717, 988
HspAI GCGC 2 cut(s) 686, 826
Kpn2I TCCGGA 1 cut(s) 630
Kzo9I GATC 2 cut(s) 43, 54
LmnI GCTCC 3 cut(s) 181, 421, 1086
Lsp1109I GCAGC 2 cut(s) 4, 830
LweI GCATC 1 cut(s) 524
MaeI CTAG 1 cut(s) 842
MaeIII GTNAC 2 cut(s) 566, 1018
MalI GATC 2 cut(s) 45, 56
MboI GATC 2 cut(s) 43, 54
MboII GAAGA 2 cut(s) 282, 901
MfeI CAATTG 1 cut(s) 140
MflI RGATCY 1 cut(s) 43
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 2 cut(s) 95, 619
MnlI CCTC 7 cut(s) 75, 78, 363, 385, 688, 766, 1059
Mph1103I ATGCAT 1 cut(s) 494
MroI TCCGGA 1 cut(s) 630
MroXI GAANNNNTTC 2 cut(s) 638, 867
MseI TTAA 4 cut(s) 440, 458, 795, 858
MslI CAYNNNNRTG 1 cut(s) 532
MspI CCGG 1 cut(s) 631
MspR9I CCNGG 3 cut(s) 96, 389, 531
MunI CAATTG 1 cut(s) 140
MvaI CCWGG 3 cut(s) 96, 389, 531
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 3 cut(s) 23, 32, 744
NdeII GATC 2 cut(s) 43, 54
NlaIII CATG 3 cut(s) 492, 717, 988
NlaIV GGNNCC 2 cut(s) 45, 423
NmuCI GTSAC 1 cut(s) 566
NsiI ATGCAT 1 cut(s) 494
PdmI GAANNNNTTC 2 cut(s) 638, 867
PfeI GAWTC 2 cut(s) 182, 463
PkrI GCNGC 3 cut(s) 16, 19, 820
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Psp6I CCWGG 3 cut(s) 94, 387, 529
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 3 cut(s) 94, 387, 529
PspN4I GGNNCC 2 cut(s) 45, 423
PspPI GGNCC 1 cut(s) 350
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 532
SaqAI TTAA 4 cut(s) 440, 458, 795, 858
SatI GCNGC 3 cut(s) 15, 18, 819
Sau3AI GATC 2 cut(s) 43, 54
Sau96I GGNCC 1 cut(s) 350
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 3 cut(s) 96, 389, 531
SetI ASST 7 cut(s) 67, 178, 337, 532, 699, 726, 1034
SfaNI GCATC 1 cut(s) 524
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 532
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SpeI ACTAGT 1 cut(s) 841
SsiI CCGC 3 cut(s) 26, 816, 828
SspI AATATT 1 cut(s) 200
SspMI CTAG 1 cut(s) 842
StyD4I CCNGG 3 cut(s) 94, 387, 529
TaaI ACNGT 3 cut(s) 169, 346, 382
TfiI GAWTC 2 cut(s) 182, 463
Tru1I TTAA 4 cut(s) 440, 458, 795, 858
Tru9I TTAA 4 cut(s) 440, 458, 795, 858
TscAI CASTG 2 cut(s) 172, 677
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 3 cut(s) 14, 17, 818
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 2 cut(s) 197, 702
TspRI CASTG 2 cut(s) 172, 677
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 6 cut(s) 49, 454, 638, 769, 919, 1039
XmnI GAANNNNTTC 2 cut(s) 638, 867
XspI CTAG 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.