RLG00000032483

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003915
Physical Location & Seq
Forward (+)
15741907 .. 15743033
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032483

Sequence Viewer

Length: 513 bp
ATGGCTATGACTAAACCTCATTACCAAGCAGAGAAAGAAGAAATCAAAAAGCTGGGAGATAACTACCGTATCAAACCACGAGGACTTAATATTGTGTGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAGGGACCGAGGATCCTGCAGAGCTGGTACAGGTCTCATGGCTGGAAGTAACAGGCTCAGTTGATGAGTTGAGACCCGGGAAGGGGTATGATCTGACCTTCGAGGTGGGGCTGGCAGGAGATGCATTTGGTTGGAAAGACATACAAGTGTTCTTGATGGCAAAGGTAGGGAAAAAGGGAAGGTACAAATGGACAAGAGTTAAACTCGATCAGGATCGGAATGACGAGAGAGTTACAATTCCTGATAACCAGAAGCTTAGCATTGATGTTCCAGCTGATACAAGGGACACCACCCTTCATTTTGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAGGGAGGTTTGAAGATTTATAATGCGAAAGTAACTGCGAGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

171

Amino Acids

19.43

Weight (kDa)

8.92

Isoelectric Point (pI)

11.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 21 - 164 6.3e-22 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AclWI GGATC 3 cut(s) 140, 153, 354
AfaI GTAC 2 cut(s) 162, 317
AfiI CCNNNNNNNGG 3 cut(s) 134, 215, 216
AgsI TTSAA 1 cut(s) 478
AluBI AGCT 4 cut(s) 52, 157, 388, 407
AluI AGCT 4 cut(s) 52, 157, 388, 407
Alw26I GTCTC 2 cut(s) 172, 199
AlwI GGATC 3 cut(s) 140, 153, 354
Ama87I CYCGRG 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 138
AsuC2I CCSGG 2 cut(s) 210, 211
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 138
BaeI ACNNNNGTAYC 2 cut(s) 52, 85
BamHI GGATCC 1 cut(s) 145
BauI CACGAG 1 cut(s) 78
BccI CCATC 1 cut(s) 283
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BcnI CCSGG 2 cut(s) 210, 211
BcoDI GTCTC 2 cut(s) 172, 199
BfmI CTRYAG 1 cut(s) 150
BlpI GCTNAGC 1 cut(s) 389
Bme1390I CCNGG 2 cut(s) 210, 211
Bme18I GGWCC 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 2 cut(s) 139, 147
BmrFI CCNGG 2 cut(s) 210, 211
BmsI GCATC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu1102I GCTNAGC 1 cut(s) 389
BpuMI CCSGG 2 cut(s) 210, 211
BsaBI GATNNNNATC 1 cut(s) 345
BsaI GGTCTC 2 cut(s) 172, 199
BsaJI CCNNGG 2 cut(s) 141, 209
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 215, 216
Bse1I ACTGG 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 345
BseDI CCNNGG 2 cut(s) 141, 209
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 134, 215, 216
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 122
BseYI CCCAGC 1 cut(s) 52
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 2 cut(s) 151, 431
BslI CCNNNNNNNGG 3 cut(s) 134, 215, 216
BsmAI GTCTC 2 cut(s) 172, 199
BsmFI GGGAC 2 cut(s) 151, 431
Bso31I GGTCTC 2 cut(s) 172, 199
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 4 cut(s) 145, 223, 340, 346
Bsp1720I GCTNAGC 1 cut(s) 389
BspCNI CTCAG 1 cut(s) 203
BspLI GGNNCC 2 cut(s) 139, 147
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 3 cut(s) 140, 153, 354
BspTNI GGTCTC 2 cut(s) 172, 199
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 141, 209
BssMI GATC 4 cut(s) 145, 223, 340, 346
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 190, 389
BstKTI GATC 4 cut(s) 148, 226, 343, 349
BstMAI GTCTC 2 cut(s) 172, 199
BstMBI GATC 4 cut(s) 145, 223, 340, 346
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 208, 209
BstSFI CTRYAG 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 138
Cfr9I CCCGGG 1 cut(s) 209
Csp6I GTAC 2 cut(s) 161, 316
CviAII CATG 1 cut(s) 171
CviJI RGCY 9 cut(s) 5, 52, 131, 157, 175, 189, 244, 388, 407
CviKI_1 RGCY 9 cut(s) 5, 52, 131, 157, 175, 189, 244, 388, 407
CviQI GTAC 2 cut(s) 161, 316
DdeI CTNAG 2 cut(s) 190, 389
DpnI GATC 4 cut(s) 147, 225, 342, 348
DpnII GATC 4 cut(s) 145, 223, 340, 346
Eco31I GGTCTC 2 cut(s) 172, 199
Eco47I GGWCC 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 209
EcoT22I ATGCAT 1 cut(s) 259
FaeI CATG 1 cut(s) 174
FaiI YATR 6 cut(s) 8, 172, 222, 275, 444, 486
FaqI GGGAC 2 cut(s) 151, 431
FatI CATG 1 cut(s) 170
GsaI CCCAGC 1 cut(s) 56
HapII CCGG 1 cut(s) 210
Hin1II CATG 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 386
HinfI GANTC 1 cut(s) 505
HpaII CCGG 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 228, 351
Hpy188III TCNNGA 3 cut(s) 286, 344, 374
HpyAV CCTTC 5 cut(s) 208, 241, 306, 437, 460
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4V TGCA 2 cut(s) 152, 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 190, 389
Hsp92II CATG 1 cut(s) 174
Kzo9I GATC 4 cut(s) 145, 223, 340, 346
LweI GCATC 1 cut(s) 244
MaeIII GTNAC 3 cut(s) 181, 364, 496
MalI GATC 4 cut(s) 147, 225, 342, 348
MboI GATC 4 cut(s) 145, 223, 340, 346
MboII GAAGA 2 cut(s) 50, 490
MflI RGATCY 1 cut(s) 145
MluCI AATT 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 245
MnlI CCTC 5 cut(s) 27, 74, 136, 229, 464
Mph1103I ATGCAT 1 cut(s) 259
MseI TTAA 2 cut(s) 87, 333
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 210, 211
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 2 cut(s) 210, 211
NdeII GATC 4 cut(s) 145, 223, 340, 346
NlaIII CATG 1 cut(s) 174
NlaIV GGNNCC 2 cut(s) 139, 147
NsiI ATGCAT 1 cut(s) 259
PsiI TTATAA 1 cut(s) 486
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 139, 147
PspPI GGNCC 1 cut(s) 138
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 2 cut(s) 162, 317
RsaNI GTAC 2 cut(s) 161, 316
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 2 cut(s) 87, 333
Sau3AI GATC 4 cut(s) 145, 223, 340, 346
Sau96I GGNCC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 210, 211
SfaNI GCATC 1 cut(s) 244
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 138
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 278
Sse9I AATT 1 cut(s) 369
SspI AATATT 1 cut(s) 91
StyD4I CCNGG 2 cut(s) 208, 209
TaaI ACNGT 1 cut(s) 68
TaqI TCGA 2 cut(s) 234, 339
TaqII GACCGA 1 cut(s) 155
TasI AATT 1 cut(s) 369
Tru1I TTAA 2 cut(s) 87, 333
Tru9I TTAA 2 cut(s) 87, 333
TspDTI ATGAA 3 cut(s) 123, 419, 459
TspMI CCCGGG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 138
XmaI CCCGGG 1 cut(s) 209
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.